A Li medicine for anti - inflammation and anti - allergy and its preparation method

Abstract

The present invention discloses a Li medicine for anti - inflammation and anti - allergy and its preparation method. The process includes: on the basis of the original Li medicine prescription, adding Yajiasong pollen newly, and developing a Li medicine for anti - inflammation and anti - allergy. Anti - inflammation, anti - allergy and antipruritic experiments are carried out on this Li medicine. The results fully prove that: Yajiasong pollen, the traditional Li medicine for anti - inflammation and anti - allergy, and the Li medicine compounded by Yajiasong pollen and the traditional Li medicine all have the effects of anti - allergy, anti - inflammation and antipruritic. In addition, the effect of the Li medicine for anti - inflammation and anti - allergy is superior to that of Yajiasong pollen and the traditional Li medicine prescription used alone, thus broadening the development space and approach for protecting and inheriting the effective prescriptions of Li medicine.

Description

Technical Field

[0001] The invention relates to an anti-inflammatory and anti-allergic Li medicine and a preparation method thereof, and belongs to the field of ethnic medicine. Background Art

[0002] The prescription is composed of four Li medicinal materials: Lantana camara, Artemisia dahurica, Artemisia dafeng and Artemisia ulmoides.

[0003] Hainan Li medicine is an important part of the Chinese nation's treasure house and a great treasure of the country's traditional ethnic medicine. The Li culture has no written language, only language, and many Li medicine secrets can only rely on oral transmission from ancestors. With the development of the times, many prescriptions are facing the risk of being lost. Therefore, the excavation, inheritance and improvement of Li medicine prescriptions is undoubtedly a rescue excavation and protection of Li medicine.

[0004] The traditional Li medicine prescription for anti-inflammatory and anti-allergic uses the external application method according to the symptoms when administering. The above four fresh leaves or the whole plant are mashed and applied to the affected area, or fresh (dry) branches and leaves or roots are boiled in water and washed with water. This method is suitable for skin itching and eczema. For those with skin erosion, an appropriate amount of the four Li medicine dry powder can be sprinkled on the affected area for treatment. However, in the actual application process, it was found that the dosage and quality of the external application and lotion were difficult to control, it was inconvenient to carry and use, it was easy to mold, and it was not easy to preserve, which limited its use. In addition, it was proved by experiments that the original prescription also had shortcomings in efficacy. Therefore, improvements were made to the above shortcomings. On the basis of the original traditional Li medicine prescription for anti-inflammatory and anti-allergic, Yajia pine pollen was added, and two dosage forms were developed using modern pharmaceutical production technology. The first is a press-type cream, which can be applied to the affected area when used. The cream has good stability, sustained release and moisturizing properties, is clean and hygienic, and is easy to carry, which significantly improves the efficacy. The second is to make Yajia Songhua Powder, which is suitable for infant eczema, thus improving the shortcomings of the original Li medicine prescription.

[0005] Pinus massoniana var. hainanensis WC Cheng & LK Fu is a variant of Pinus massoniana, mainly distributed in Hainan Province. Pinus massoniana pollen has a long history of application in the Li ethnic group-inhabited areas of Hainan. It is mainly used for external use and is suitable for children's eczema, skin itching and ulcers. Pinus massoniana pollen has anti-inflammatory, analgesic, antibacterial and anti-allergic pharmacological activities. Its main functions are drying dampness and astringency, and it is used for traumatic bleeding and impetigo. Pinus massoniana pollen has anti-allergic, anti-inflammatory and antipruritic effects. Therefore, Pinus massoniana pollen is compounded with traditional Li medicine prescriptions that can resist inflammation and allergy, and an anti-inflammatory and anti-allergic Li medicine is developed. According to literature search, there is no report on the use of Pinus massoniana pollen in medicine.

[0006] Lantana is a plant of the genus Lantana in the Verbenaceae family, Lantana camara L., also known as five-color plum, Ruyi grass, seven-change flower, and stinky grass. Its roots are cool in nature and sweet in taste, its leaves are cold in nature and bitter and pungent in taste, its flowers are cool in nature and sweet and light in taste, and it enters the lung and liver meridians. It has the effects of clearing away heat and detoxifying, dispersing blood stasis and reducing swelling, and dispelling wind and relieving itching. Its chemical components include volatile oils, triterpenes, flavonoids, and phenylethanol glycosides.

[0007] Euphorbia biahirta, also known as Euphorbia biahirta, Euphorbia biahirta, is the dried whole herb of Euphorbia biahirta L., Euphorbiaceae. It tastes spicy, sour, cool in nature, and slightly toxic. It enters the lung, bladder, and large intestine meridians. It is distributed in Guangdong, Hainan, Yunnan, and other places in my country. It has the effects of promoting dampness and relieving itching, clearing away heat and detoxifying, and promoting lactation. It is mainly used to treat eczema, dermatitis, skin itching, lung abscess, and breast abscess. Euphorbia biahirta mainly contains flavonoids, coumarins, lignans, triterpenes, steroids, tannins, alkaloids, and other chemical components.

[0008] Artemisia balsamifera, also known as Blumea balsamifera, is the dried aerial part of the Asteraceae plant Blumea balsamifera. It has the effects of warming the middle and activating blood circulation, regulating menstruation, and removing wind and dampness. This medicinal material mainly contains volatile oil (monoterpenoid), flavonoids, and sesquiterpenoid components.

[0009] Embelia burm is the dried leaves of Embelia laeta (L.) Mez and E. ribes Burm, plants of the genus Embelia Burm. f. of the family Myrsinaceae. Embelia burm leaves can treat vitamin C deficiency, anemia, etc. They can also be mashed and applied to bruises and swellings, and the juice can be applied to itchy skin. Embelia burm is rich in quinones, flavonoids, steroids, phenolic acids, volatile oils and other ingredients. Summary of the invention

[0010] In view of this, the present invention provides an anti-inflammatory and anti-allergic Li medicine and a preparation method, which facilitates the subsequent excavation and exploration of traditional Li medicine prescriptions, and also broadens the development space and path for the protection and inheritance of Li medicine prescriptions.

[0011] In order to achieve the above purpose, the following technical solutions are adopted:

[0012] An anti-inflammatory and anti-allergic Li medicine and a preparation method thereof, comprising the following steps:

[0013] Preparation of the Herba Lysimachiae Extract

[0014] Lantana camara extract: Take 100 g of dried Lantana camara roots or leaves and crush them using a pulverizer. Place the crushed medicinal materials in a reflux extraction tank, add 50% (V / V) ethanol at 70 °C for reflux extraction, for a total of 3 times, with each reflux for 1 h. Add 500 mL of ethanol for the first time, 300 mL for the second time, and 200 mL for the third time. After combining the three extraction solutions, distill and recover ethanol under reduced pressure, concentrate the extraction solution, and finally freeze-dry the extraction solution to obtain the extract.

[0015] Euphorbia hirta extract: Air-dry the collected whole Euphorbia hirta plants and crush them with a pulverizer. Store the obtained plant powder in a sealed bag in a light-shielded place for later use. Weigh 100 g of the sample and place it in a reflux extraction tank. Add 50% (V / V) ethanol at 70 °C for reflux extraction 3 times, with each reflux for 1 h. Add 500 mL of the above ethanol for the first time, 300 mL for the second time, and 200 mL for the third time. Combine the three extraction solutions, distill and recover ethanol under reduced pressure, concentrate the extraction solution, and finally freeze-dry the extraction solution to obtain the extract.

[0016] Blumea balsamifera extract: Weigh and crush 100 g of the dried whole Blumea balsamifera plants. Place the obtained powder in a reflux extraction tank. Add 50% (V / V) ethanol at 70 °C for reflux extraction 3 times, with each reflux for 1 h. Add 500 mL of the above ethanol for the first time, 300 mL for the second time, and 200 mL for the third time. Combine the three extraction solutions, distill and recover ethanol under reduced pressure, concentrate the extraction solution, and finally freeze-dry the extraction solution to obtain the extract.

[0017] Embelia laeta extract: Take 100 g of dried Embelia laeta leaves, crush them, and place them in a reflux extraction tank. Add 50% (V / V) ethanol at 70 °C for reflux extraction 3 times, with each reflux for 1 h. Add 500 mL of the above ethanol for the first time, 300 mL for the second time, and 200 mL for the third time. Combine the three extraction solutions, distill and recover ethanol under reduced pressure, concentrate the extraction solution, and finally freeze-dry the extraction solution to obtain the extract.

[0018] Preparation of Pinus massoniana Lamb. var. hainanensis pollen

[0019] Pinus massoniana Lamb. var. hainanensis pollen: The unbroken Pinus massoniana Lamb. var. hainanensis pollen was collected from Hainan Bawangling National Nature Reserve and identified as Pinus massoniana Lamb. var. hainanensis pollen by Researcher Wang Yong of the School of Pharmacy, Hainan Medical University.

[0020] Method: Weigh 3g of unbroken wall Pinus japonici pollen sample and put it into a 50mL grinding tube (so that the sample volume accounts for about 1 / 3 of the total volume of the grinding tube), then put in the grinding glass ball, cover the tube tightly, and after balancing, put the grinding tube and the grinding tool into a -20℃ refrigerator. After the sample and the grinding tool are cooled to -20℃, put the sample and the grinding tool into a grinder for grinding. Under the conditions of grinding frequency of 58Hz, grinding time of 290s and grinding ball diameter of 4mm, the wall breaking rate of Pinus japonici pollen can reach (93.88±0.78)%, and finally Pinus japonici pollen is obtained.

[0021] In order to explore the composition of pine pollen, Beijing Zhongke Baice Testing Technology Co., Ltd. was commissioned to conduct testing:

[0022] 1. Detection of volatile components of Yakapine pollen: Gas chromatography-mass spectrometry (GCMS), Agilent, 7890B-5977B Weigh 0.382g of all samples into a headspace bottle. Solid phase microextraction (solid phase microextraction fiber: DVB / CAR / PDMS (LabTech)) was performed at 45°C, with a shaking time of 15min, an adsorption time of 30min, and a desorption test at 250°C for 5min. Column: DB-5ms+DG 30 mx 0.25 mm x 0.2.

[0023] The volatile components of pine pollen were tested by Beijing Zhongke Baice Testing Technology Co., Ltd. (report number: ZKBC240524-394):.

[0024]

[0025]

[0026] The conclusion is: it contains ingredients that clear heat, relieve pain and inhibit bacteria.

[0027] 2. Test results of non-volatile components of P. jasminoides pollen.

[0028] 1. Dry Sample Extraction

[0029] (1) The biological samples were placed in a freeze dryer (Scientz-100F) and vacuum-frozen for 63 h;

[0030] (2) Grinding (30 Hz, 1.5 min) into powder using a grinder (MM 400, Retsch);

[0031] (3) Weigh 50 mg of the sample powder using an electronic balance (MS105DΜ), add 1200 μL of the internal standard extraction solution of 70% methanol pre-cooled to -20 °C (if less than 50 mg, add according to the ratio of 1200 μL of the extraction agent per 50 mg of the sample). The internal standard extraction solution is prepared by dissolving 1 mg of the standard in 1 mL of 70% methanol water to prepare a 1000 μg / mL stock solution of the standard, and the 1000 μg / mL stock solution is further diluted with 70% methanol to prepare a 250 μg / mL internal standard solution;

[0032] (4) Vortex once every 30 minutes for 30 seconds each time, for a total of 6 times;

[0033] (5) After centrifugation (at a speed of 12000 rpm for 3 minutes), aspirate the supernatant, filter the sample with a microporous membrane (0.22 μm pore size), and store it in a sample vial for UPLC-MS / MS analysis.

[0034] 2. Chromatographic conditions

[0035] (1) Chromatographic column: Waters ACQUITY Premier HSS T3 Column 1.8 µm, 2.1 mm * 100 mm

[0036] (2) Mobile phase A: 0.1% formic acid / water; Mobile phase B: 0.1% formic acid / acetonitrile

[0037] (3) Column temperature of the instrument: 40 °C; Flow rate: 0.4 mL / min; Injection volume: 4 μL.

[0038]

[0039] 3. Mass spectrometry conditions

[0040]

[0041]

[0042]

[0043]

[0044]

[0045]

[0046]

[0047]

[0048] The conclusion is that there are many anti-inflammatory, antibacterial and anti-allergic components.

[0049] Prepare a Li medicine for anti-inflammation and anti-allergy

[0050] Embodiment 1

[0051] 60% of Pinus massoniana Lamb. var. hainanensis pollen, 15% of Lantana camara L. extract, 10% of Euphorbia hirta L. extract, 10% of Blumea balsamifera (L.) DC. extract, 5% of Embelia laeta (Lam.) Mez extract,

[0052] The total weight reaches 100%. Grind the above medicine and mix it evenly to make tablets, each tablet weighing 1 g. Take 2 tablets orally before meals, 3 times a day.

[0053] Embodiment 2

[0054] 60% of Pinus massoniana Lamb. var. hainanensis pollen, 15% of Lantana camara L. extract, 10% of Euphorbia hirta L. extract, 10% of Blumea balsamifera (L.) DC. extract, 5% of Embelia laeta (Lam.) Mez extract

[0055] The total weight reaches 100 g. Grind the above medicine, soak it in rice wine and then filter to make a spray. Each bottle is 20 g. Use an appropriate amount when needed and spray it on the affected area of the patient several times a day.

[0056] Embodiment 3

[0057] 60 g of Pinus massoniana Lamb. var. hainanensis pollen, 15 g of Lantana camara L. extract, 10 g of Euphorbia hirta L. extract, 10 g of Blumea balsamifera (L.) DC. extract, 5% of Embelia laeta (Lam.) Mez extract

[0058] The total weight reaches 100 g. Grind the above medicine and mix it evenly to make a powder. Take an appropriate amount of the powder and sprinkle it on the affected area, 3 times a day.

[0059] Embodiment 4

[0060] 60 g of Pinus massoniana Lamb. var. hainanensis pollen, 15 g of Lantana camara L. extract, 10 g of Euphorbia hirta L. extract, 10 g of Blumea balsamifera (L.) DC. extract, 5 g of Embelia laeta (Lam.) Mez extract

[0061] The total weight reaches 100 g. Grind the above medicine and mix it evenly to make a powder, then add 100 g of ordinary honey and mix well. Divide it evenly into 50 portions to make honey pills, each pill weighing 4 g. The patient takes 1 pill orally before meals, 3 times a day.

[0062] Embodiment 5

[0063] 60 g of Pinus massoniana Lamb. var. hainanensis pollen, 15 g of Lantana camara L. extract, 10 g of Euphorbia hirta L. extract, 10 g of Blumea balsamifera (L.) DC. extract, 5 g of Embelia laeta (Lam.) Mez extract

[0064] The total weight reaches 100g, and the above-mentioned drugs are ground into powder, mixed thoroughly and evenly, made into powder, and loaded into medicinal glutinous rice capsules. The net weight of each capsule is 0.5g. The patient takes 4 capsules orally before each meal, 3 times a day.

[0065] Implementation Plan 6

[0066] 60g of pine pollen, 15g of Lantana camara extract, 10g of Artemisia selengensis extract, 10g of Artemisia selengensis extract, 5g of Atractylodes lancea extract

[0067] The total weight reaches 100g, and the above medicines are ground into powder, mixed thoroughly and evenly, made into granules, packed in small medicinal paper bags and sealed. The net weight of each bag is 2g. The patient takes 1 bag before each meal, 3 times a day.

[0068] Implementation Plan 7

[0069] According to the following weight ratio: 60g of pine pollen, 15g of Lantana camara extract, 10g of Artemisia selengensis extract, 10g of Artemisia selengensis extract, 5g of Atractylodes lancea extract

[0070] The above-mentioned drugs are ground into fine powder, mixed thoroughly and evenly, and made into tinctures, creams, gels, ointments, jasmine powder, plasters and other external preparations.

[0071] It can be seen from the above technical scheme that compared with the prior art, the present invention discloses an anti-inflammatory and anti-allergic Li medicine and a preparation method. On the basis of the traditional anti-inflammatory and anti-allergic Li medicine prescription, yagapine pollen was added to develop a Li medicine. After anti-allergic, anti-inflammatory and antipruritic experiments on mice, the results showed that the anti-inflammatory and anti-allergic effects of the traditional Li medicine prescription and yagapine pollen were better than those of the traditional Li medicine prescription and yagapine pollen. This provides scientific data for the protection and inheritance of Li medicine prescriptions, secondary innovation and the construction of Li medicine brands. BRIEF DESCRIPTION OF THE DRAWINGS

[0072] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0073] Figure 1 Comparison of mouse ear swelling for anti-allergic test results

[0074] Figure 2 Anti-inflammatory test results DETAILED DESCRIPTION

[0075] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the implementation cases described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0076] Experimental instruments and equipment

[0077] Model: 800A Fang Taitai multifunctional pulverizer Dongguan Fang Tai Electric Co., Ltd., refrigerator (Qingdao Haier Co., Ltd.), BCD-26TS digital display constant temperature water bath (Jintan Shenglan Instrument Co., Ltd.), KW-1000DC electric constant temperature blast drying oven (Shanghai Jinghong Experimental Equipment Co., Ltd.), DHG-9030AXinyi-48 high-throughput tissue grinder (with freezing module) (Ningbo Xinyi Ultrasonic Equipment Co., Ltd.), WH-3 micro vortex mixer (Haihuxi Analytical Instrument Factory Co., Ltd.), E220G biological microscope (McOdie Industrial Group China Co., Ltd.), PGX-280A-12HM ultrapure water machine (Sichuan Youpu Ultrapure Technology Co., Ltd.), B0510C rotary evaporator (Chongqing Yamato Technology Co., Ltd.), Junde brand freeze dryer FD-503, heat reflux device, FA1204G balance (Changzhou Wantai Balance Instrument Co., Ltd.), KQ-B electronic temperature control universal furnace (Tianjin Test Instrument Co., Ltd.).

[0078] Experimental consumables and chemical reagents

[0079] Hydrocortisone butyrate cream was produced by Zhejiang Xianju Pharmaceutical Co., Ltd. with a batch number of ML2316. Compound dexamethasone acetate cream was produced by Hubei Ketian Pharmaceutical Co., Ltd. with a product batch number of 230210. 1-Chloro-2.4-dinitrochlorobenzene was purchased from Beijing Bailingwei Technology Co., Ltd. with a product number of 443475. 4-Aminopyridine (4-AP) and acetone were provided by Hainan Tropical Ocean University, and xylene was purchased from Guangdong Fine Chemical Engineering Technology Research and Development Center with a batch number of 2310242; Li Danlan Herbal Antibacterial Gel was produced by Li Danlan (Hainan) Biomedical Technology Co., Ltd. with a batch number of HM24-03Y02D1. Unbroken wall pine pollen was collected from Bawangling, Hainan Province. Pine pollen, traditional Li medicine for anti-inflammatory and anti-allergic effects, anti-inflammatory and anti-allergic Li medicine, sacha inchi oil, and distilled water were all made in the laboratory.

[0080] Experimental methods

[0081] (1) Drug preparation

[0082] Hydrocortisone butyrate cream: Accurately weigh 0.12 g and apply 0.12 g to each mouse.

[0083] Compound Dexamethasone Acetate Cream: Accurately weigh 0.12 g and apply 0.12 g externally to each mouse.

[0084] Lidanlan Herbal Bacteriostatic Gel: Accurately weigh 0.12 g and apply 0.12 g externally to each mouse.

[0085] Yajia Pine Pollen: Accurately weigh 0.6 g and place it in a 100 mL volumetric flask. Add pure water to the mark and dissolve and shake well. Apply 0.2 mL (containing 1.2 mg) externally to each mouse.

[0086] Traditional Li Medicine for Anti - inflammation and Anti - allergy: Accurately weigh 0.6 g and place it in a 100 mL volumetric flask. Add pure water to the mark and dissolve and shake well. Apply 0.2 mL (containing 1.2 mg) externally to each mouse.

[0087] Li Medicine for Anti - inflammation and Anti - allergy: Accurately weigh 0.6 g and place it in a 100 mL volumetric flask. Add pure water to the mark and dissolve and shake well. Apply 0.2 mL (containing 1.2 mg) externally to each mouse.

[0088] (2)Experimental animals: ICR white mice were purchased from Hunan Slake Jingda Experimental Animal Co., Ltd., SPF level, license number SYXk(Xiang)2019 - 0004. The depilatory cream was purchased from Shenzhen Mengguang Cosmetics Co., Ltd., batch number: 2023082305. 8 - mm hole punch, funnel, flask, beaker, glass rod, gauze, filter paper, measuring cylinder, mouse cage.

[0089] Experimental methods

[0090] I. Anti - allergy experiment:

[0091] 1. Modeling experiment: Take 60 healthy mice and randomly divide them into 5 groups: 12 in the model control group, 12 in the positive control group, 12 in the Yajia Pine Pollen experimental group, 12 in the traditional Li Medicine control group for anti - inflammation and anti - allergy, and 12 in the Li Medicine experimental group for anti - inflammation and anti - allergy. Depilate about 2.5 cm × 2.5 cm on the abdomen of each group of mice. After depilation, sensitize the abdomen of the mice once with 50 μL of 7% 1 - chloro - 2,4 - dinitrobenzene - acetone - meitenga oil solution (3:1, V / V), and repeat the sensitization the next day. On the 6th, 9th, 10th, 13th, and 16th days of the experiment, stimulate the inner and outer sides of the right ear of the mice with 20 μL of 0.5% 1 - chloro - 2,4 - dinitrobenzene - acetone - meitenga oil solution for multiple times. After 10 days, take the appearance of redness, swelling, hypertrophy and other pathological symptoms in the right ear of the mice as the standard for successful modeling.

[0092] 2. Experiments after successful modeling: Exclude the mice in which the modeling was not successful. Take 50 successfully modeled mice and divide them into 5 groups: A. Positive control group with 10 mice, apply hydrocortisone butyrate cream to the right ear of the mice; B. Model group with 10 mice, apply distilled water to the right ear of the mice; C. Jacaranda acutifolia pollen experimental group with 10 mice, apply Jacaranda acutifolia pollen to the right ear of the mice; D. Traditional Li medicine control group for anti-inflammatory and anti-allergy with 10 mice, apply traditional Li medicine to the right ear of the mice; E. Li medicine experimental group for anti-inflammatory and anti-allergy with 10 mice, apply Li medicine to the right ear of the mice. Another 10 mice are used as the normal control group, apply distilled water to the right ear of the mice, apply once in the morning and once in the afternoon to each of the above groups every day, and continuously apply for 7 days. 30 minutes after the last administration, except for the normal control group, other groups are used to stimulate allergic reactions by applying 0.5% 1-chloro-2,4-dinitrobenzene-acetone-moringa oil to the right ear of the mice. After 20 hours, the mice are sacrificed by dislocation, the auricles of both ears are cut off, round ear pieces are punched at the same position on the left and right ears with an 8 mm punch, weighed using an electronic balance, the thickness in the middle of the left and right ears of the mice is measured, the ear swelling degree is calculated, and the parallel experiment is carried out 3 times. The calculation formula for the ear swelling degree (%) is: (right ear thickness - left ear thickness) × 100%.

[0093] Results of anti-allergy experiment:

[0094] Table 1 Comparison of ear swelling degrees of mice ( ,%, n = 10)

[0095]

[0096] Compared with the normal control group, # P < 0.05 or ## P < 0.01; compared with the model group, * P < 0.05 or ** P < 0.01.

[0097] Conclusion: Compared with the model group, in the positive control group (hydrocortisone butyrate cream), Jacaranda acutifolia pollen experimental group, traditional Li medicine control group for anti-inflammatory and anti-allergy, and Li medicine experimental group for anti-inflammatory and anti-allergy, the ear swelling degrees of the mice were significantly reduced at the 1st, 2nd, and 3rd stimulations (the 10th, 13th, and 16th days) after administration.

[0098] II. Anti-inflammatory experiment

[0099] Sixty healthy mice were divided into six groups: (1) In the normal control group, 10 mice had distilled water applied to their right ears; (2) In the model control group, 10 mice had xylene (0.2 mL / mouse) applied to both the front and back of their right ears to induce inflammation; (3) In the positive control group, 10 mice had xylene (0.2 mL / mouse) applied to both the front and back of their right ears to induce inflammation; (4) In the Pinus massoniana Lamb. pollen experimental group, 10 mice had xylene (0.2 mL / mouse) applied to both the front and back of their right ears to induce inflammation; (5) In the traditional Li medicine control group for anti - inflammation and anti - allergy, 10 mice had xylene (0.2 mL / mouse) applied to both the front and back of their right ears to induce inflammation; (6) In the Li medicine experimental group for anti - inflammation and anti - allergy, 10 mice had xylene (0.2 mL / mouse) applied to both the front and back of their right ears to induce inflammation. At 1, 2, and 3 hours after inflammation induction, each group was topically administered the corresponding dose of the drug to the right ear. The normal control group and the model group were topically administered distilled water. Four hours later, the mice were sacrificed by cervical dislocation, and the left and right ears were cut along the auricle baseline. At the same position on both the left and right ears, circular ear pieces were taken with an 8 - mm punch. Then, the weights were measured on an analytical balance. The difference in the weights of the two ear pieces was used as the degree of inflammatory swelling, and the swelling inhibition rate was calculated. Inhibition rate % = (control group - drug - administered group) / swelling degree of the model control group × 100%.

[0100] Table 2 Ear swelling experiment in mice induced by xylene ( , n = 10)

[0101]

[0102] Note: a Compared with the normal control group, P < 0.05; b Compared with the model group, P < 0.05.

[0103] The effects of the Pinus massoniana Lamb. pollen experimental group, the traditional Li medicine control group for anti - inflammation and anti - allergy, and the Li medicine experimental group for anti - inflammation and anti - allergy on ear swelling in mice induced by xylene were studied. Compared with the normal control group, the ear swelling degree of the model control group was significantly increased, and the difference was statistically significant (P < 0.05), indicating that the xylene - induced inflammation model was successfully established. The ear swelling degrees of the mice in each drug - administered group were significantly lower than those of the model control group, and the differences were all statistically significant (P < 0.05). Compared with the normal control group, the differences were not statistically significant. This indicates that the Pinus massoniana Lamb. pollen experimental group, the traditional Li medicine control group for anti - inflammation and anti - allergy, and the Li medicine experimental group for anti - inflammation and anti - allergy can inhibit ear swelling in mice induced by xylene and restore the ear swelling to normal. Among them, the effect of the Li medicine experimental group for anti - inflammation and anti - allergy was better than that of the Pinus massoniana Lamb. pollen and the traditional Li medicine control group for anti - inflammation and anti - allergy, but still lower than that of the positive control group.

[0104] III. Pruritus - relieving experiment

[0105] Sixty healthy mice, with 30 males and 30 females, weighing 18 - 22 g, were randomly divided into six groups: (1) normal control group; (2) model control group; (3) positive control group (Compound Dexamethasone Acetate Cream); (4) Yajiasong pollen experimental group; (5) traditional Li medicine control group for anti - inflammation and anti - allergy; (6) Li medicine experimental group for anti - inflammation and anti - allergy, with 10 mice in each group. The hair on the back (2.5 cm × 2.5 cm) was removed with depilatory cream, and the test drugs were treated as follows: (1) The normal control group was smeared with distilled water; (2) The model control group was smeared with distilled water; (3) The positive control group was smeared with Compound Dexamethasone Cream; (4) The Yajiasong pollen experimental group was smeared with Yajiasong pollen; (5) The traditional Li medicine control group for anti - inflammation and anti - allergy was smeared with traditional Li medicine; (6) The Li medicine experimental group for anti - inflammation and anti - allergy was smeared with Li medicine. Each group was treated once a day for 7 days.

[0106] Inducing itching: 40 minutes after the last administration, 4 - AP (prepared into 0.02% with normal saline) solution was subcutaneously injected into the nape and back of the mice at a dose of 0.01 ml / g. The mice in the normal control group were injected with normal saline, while the mice in the model group, positive control group, Yajiasong pollen experimental group, traditional Li medicine control group for anti - inflammation and anti - allergy, and Li medicine experimental group for anti - inflammation and anti - allergy were all injected with 4 - AP.

[0107] Observation of itching reaction: The number of times of self - licking behavior of the mice was recorded within 10 minutes after the start of observation. Self - licking behavior refers to the action of the mice repeatedly turning their heads to lick both sides of the back. The behavior of continuous self - licking with a short pause was counted as 1 time of self - licking. The latency, duration, and number of self - licking times of the mice's self - licking reaction were observed, and the reaction inhibition rate was calculated at the same time. The calculation formula for the inhibition rate is: Inhibition rate = (Number of self - licking times in the model control group - Number of self - licking times in the drug - administered group) / Number of self - licking times in the model control group × 100%.

[0108] Table 3 Itching relief experiment ( , n = 10)

[0109]

[0110] Note: Compared with the model control group, * P < 0.05, ** P < 0.01.

[0111] Compared with the model control group, the number of self - licking times of the mice in the positive control group, Yajiasong pollen experimental group, traditional Li medicine control group for anti - inflammation and anti - allergy, and Li medicine experimental group for anti - inflammation and anti - allergy within 30 minutes were all significantly reduced (P < 0.05 or P < 0.01). In addition, the number of scratching times of the mice in the positive control group, Yajiasong pollen experimental group, traditional Li medicine control group for anti - inflammation and anti - allergy, and Li medicine experimental group for anti - inflammation and anti - allergy within 30 minutes were also all significantly reduced (P < 0.01).

Claims

1. An anti-inflammatory and anti-allergic medicine, characterized in that: The specific prescription composition is as follows: 60% of pine pollen, 15% of Lantana camara extract, 10% of Artemisia selengensis extract, 10% of Artemisia argyi extract, and 5% of Achyranthes bidentata extract, wherein the extracts are all ethanol extracts.

2. The method for preparing the Li medicine according to claim 1, characterized in that: The extraction methods of the extracts are all to reflux leaching under heating conditions, recover ethanol, and freeze-dry the extracts.

3. The Li medicine according to claim 1 or the Li medicine obtained by the preparation method according to claim 2, characterized in that: The above-mentioned Li medicine can be made into powder or gel.

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