Detection device, analysis method and analysis device for nucleated red blood cells, and storage medium

Abstract

The invention discloses detection equipment, an analysis device and an analysis method for nucleated red blood cells and a storage medium. The equipment comprises a light-emitting assembly, a detection module and a control module, the flow control assembly comprises a sample introduction channel and a flow chamber which are connected with each other, and the flow chamber is internally provided with an illumination area through which a light-transmitting single particle to be detected passes; a scattering detection assembly; and the image processing assembly is connected with the scattering detection assembly and is used for generating a forward scattering light-lateral information scatter diagram containing nucleated red blood cell scatter points according to the forward scattering light signal and the lateral scattering light signal. According to the scheme, leukocytes can be classified and detected under the condition that a fluorescent reagent is not used, the preset area of the nucleated red blood cell accumulation area is positioned by analyzing the forward scattered light-lateral information scatter diagram of nucleated red blood cell scatter points, and information about whether the number of nucleated red blood cells in the preset area is abnormal or not is determined; according to the scheme, the detection complexity and cost are low, and information related to nucleated red blood cells can be rapidly, simply and conveniently provided.

Description

Technical Field

[0001] The present application relates to the field of medical equipment, and specifically to a detection device, an analysis method, an analysis apparatus and a storage medium for nucleated red blood cells. Background Art

[0002] Nucleated red blood cells normally exist in the bone marrow. In normal people, there are no nucleated red blood cells in blood samples other than the bone marrow. The appearance of nucleated red blood cells in blood samples is due to the release of immature bone marrow erythroid cells into the blood samples. Their appearance in blood samples is related to blood diseases. Therefore, it is necessary to check whether there are nucleated red blood cells in the blood clinically.

[0003] In the prior art, in order to count the nucleated red blood cells, specific dyes, fluorescent probes or other markers are often used to mark the nucleated red blood cells. However, this method may increase the complexity and cost of the detection instrument. Summary of the invention

[0004] In order to solve the above problems, the present application provides a detection device, analysis method, analysis apparatus and storage medium for nucleated red blood cells.

[0005] The first aspect of the present application provides a nucleated red blood cell detection device, the device comprising: a light-emitting component for emitting light of a predetermined wavelength; a fluidic control component, the fluidic control component comprising an injection channel and a flow chamber connected to each other, the flow chamber being provided with a light-transmitting illumination area through which a single particle to be detected passes; a scattering detection component, for detecting forward scattered light and side scattered light formed after the single particle to be detected passes through the illumination area after being irradiated by light, thereby obtaining a forward scattered light signal and a side scattered light signal; an image processing component, connected to the scattering detection component, for generating a forward scattered light-side information scatter plot containing nucleated red blood cell scatter points according to the forward scattered light signal and the side scattered light signal; wherein a preset area adjacent to a white blood cell aggregation area in the forward scattered light-side information scatter plot is defined as a nucleated red blood cell aggregation area.

[0006] In some embodiments, the scattering detection assembly includes a first scattering detection component and a second scattering detection component. The first scattering detection component is used to detect and obtain a forward scattered light signal; and the second scattering detection component is used to detect and obtain a side scattered light signal.

[0007] In one embodiment, the nucleated red blood cell detection device also includes a sampling component, which is used to input the processed blood sample in one portion or two portions of equal size into the fluidic control component successively, wherein, in the process of inputting the two portions of equal size into the fluidic control component successively, the forward scattered light information of each cell is collected from the first blood sample, and the side scattered light information of each cell is collected from the second blood sample.

[0008] In one embodiment, the image processing component is further used to analyze the forward scattered light-side information scatter plot, and determine the area where the characteristic particle group does not appear in the forward scattered light-side information scatter plot of the normal blood sample, but the characteristic particle group appears in the forward scattered light-side information scatter plot of the single particle to be tested as the nucleated red blood cell aggregation area.

[0009] In one embodiment, the image processing component is used to count the nucleated red blood cells in the nucleated red blood cell aggregation area to obtain a sample count, and determine whether the number of the sample count is within a predetermined range.

[0010] In one embodiment, the nucleated red blood cell detection device includes an alarm component connected to the image processing component, which is used to perform alarm processing when the number of sample counts is not within a predetermined range and the number of nucleated red blood cells in the blood sample is determined to be abnormal.

[0011] The second aspect of the present application provides a method for analyzing nucleated red blood cells, the method comprising: obtaining forward scattered light information and side scattered light information of cells in a blood sample when the blood sample passes through a detection area of ​​a detection device; generating a forward scattered light-side scatter plot containing nucleated red blood cell scatter points based on the forward scattered light information and the side scattered light information; obtaining a nucleated red blood cell aggregation area of ​​the forward scattered light-side scatter plot based on the forward scattered light-side scatter plot containing nucleated red blood cell scatter points; counting the nucleated red blood cells in the nucleated red blood cell aggregation area to obtain a sample count, and determining whether the number of the sample count is within a predetermined range; in response to the number of the sample count not being within the predetermined range, determining that the number of nucleated red blood cells in the blood sample is abnormal, and performing alarm processing.

[0012] In one embodiment, before the step of obtaining forward scattered light information and side scattered light information of cells in the blood sample when the blood sample passes through the detection area of ​​the cytometer, the method also includes: obtaining an initial blood sample containing white blood cells; mixing the initial blood sample with a predetermined reagent in a certain proportion to form a blood sample treated with the predetermined reagent, wherein the predetermined reagent can dissolve the red blood cells in the initial blood sample.

[0013] In one embodiment, a forward scattered light-side scatter plot including nucleated red blood cell scatter points is generated according to the forward scattered light information and the side scattered light information. The method also includes: dividing the white blood cells in the blood sample into at least four categories: lymphocyte group, monocyte group, eosinophil group, and neutrophil group according to the forward scattered light information and the side scattered light information.

[0014] In one embodiment, the method further comprises the steps of obtaining a nucleated red blood cell aggregation area of ​​a forward scattered light-side scatter plot based on a forward scattered light-side scatter plot containing nucleated red blood cell scatter points: obtaining position data of a lymphocyte group and a blood shadow area, wherein the lymphocyte group and the blood shadow area are obtained by image processing or computer vision; and determining the position of the nucleated red blood cell aggregation area based on the position data of the lymphocyte group and the blood shadow area.

[0015] According to a third aspect of the present application, there is provided a device for analyzing nucleated red blood cells, the device comprising: a first acquisition module, for acquiring forward scattered light information and side scattered light information of cells in a blood sample when the blood sample passes through a detection zone of a detection device; a scatter plot generation module, for generating a forward scattered light-side scatter plot containing nucleated red blood cell scatter points based on the forward scattered light information and the side scattered light information; a second acquisition module, for acquiring a nucleated red blood cell aggregation area of ​​a forward scattered light-side scatter plot based on the forward scattered light-side scatter plot containing nucleated red blood cell scatter points; a counting module, for counting the nucleated red blood cells in the nucleated red blood cell aggregation area to obtain a sample count, and determining whether the number of the sample count is within a predetermined range; and an alarm module, for determining that the number of nucleated red blood cells in the blood sample is abnormal in response to the number of the sample count not being within the predetermined range, and performing an alarm process.

[0016] A fourth aspect of the present application provides a computer-readable storage medium having program instructions stored thereon, which implement any of the above-mentioned methods for analyzing nucleated red blood cells when the program instructions are executed by a processor.

[0017] In the above scheme, the nucleated red blood cell detection device includes a light-emitting component, a fluidic control component, a scattering detection component, and an image processing component. By detecting the forward scattered light and side scattered light formed after the single particle to be tested passes through the illumination area, and using the image processing component to generate a forward scattered light-side information scatter plot, the generated forward scattered light-side information scatter plot containing the nucleated red blood cell scatter points is analyzed to determine the nucleated red blood cell aggregation area adjacent to the white blood cell aggregation area in the scatter plot. By analyzing the number of nucleated red blood cells in the nucleated red blood cell aggregation area, information on whether the number of nucleated red blood cells is abnormal can be provided. In addition, when performing detection by the nucleated red blood cell detection device, there is no need to add a fluorescent agent to the sample, the complexity and cost of the detection are low, and information about nucleated red blood cells can be provided quickly and easily. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The drawings herein are incorporated into the specification and constitute a part of the specification. These drawings illustrate embodiments consistent with the present application and are used together with the specification to illustrate the technical solution of the present application.

[0019] Figure 1 It is a schematic structural diagram of an embodiment of a nucleated red blood cell detection device shown in the present application;

[0020] Figure 2 A schematic diagram of an exemplary embodiment of a method for analyzing nucleated red blood cells shown in the present application;

[0021] Figure 3 yes Figure 2 A schematic flow chart of an exemplary embodiment of the method for analyzing nucleated red blood cells before step S110 is shown;

[0022] Figure 4 yes Figure 2 A schematic flow chart of an exemplary embodiment of step S130 in the method for analyzing nucleated red blood cells is shown;

[0023] Figure 5 is a schematic diagram of obtaining the nucleated red blood cell aggregation area shown in the present application;

[0024] Figure 6 It is a structural schematic diagram of an embodiment of a nucleated red blood cell analysis device shown in the present application;

[0025] Figure 7 It is a structural diagram of an embodiment of the computer storage medium of the present application. DETAILED DESCRIPTION

[0026] The following embodiments of the technical solution of the present application are described in detail in conjunction with the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present application, and are therefore only used as examples, and cannot be used to limit the scope of protection of the present application.

[0027] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by technicians in the technical field to which this application belongs; the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" in the specification and claims of this application and the above-mentioned figure descriptions and any variations thereof are intended to cover non-exclusive inclusions.

[0028] In the description of the embodiments of the present application, the technical terms "first", "second", etc. are only used to distinguish different objects, and cannot be understood as indicating or implying relative importance or implicitly indicating the number, specific order or primary and secondary relationship of the indicated technical features. In the description of the embodiments of the present application, the meaning of "multiple" is more than two, unless otherwise clearly and specifically defined. The specific features, structures or characteristics described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of this phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0029] In the description of the embodiments of the present application, the term "and / or" is only a description of the association relationship of the associated objects, indicating that there may be three relationships. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. In addition, the character " / " in this article generally indicates that the associated objects before and after are in an "or" relationship.

[0030] In the description of the embodiments of the present application, the term "multiple" refers to more than two (including two). Similarly, "multiple groups" refers to more than two groups (including two groups), and "multiple pieces" refers to more than two pieces (including two pieces).

[0031] In the description of the embodiments of the present application, the technical terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, which are only for the convenience of describing the embodiments of the present application and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the embodiments of the present application.

[0032] In the description of the embodiments of the present application, unless otherwise clearly specified and limited, technical terms such as "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and it can be the internal connection of two elements or the interaction relationship between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the embodiments of the present application can be understood according to the specific circumstances.

[0033] See also Figure 1 , Figure 1 1 is a schematic structural diagram of an embodiment of a nucleated red blood cell detection device 100 shown in the present application.

[0034] The nucleated red blood cell detection device 100 provided in the embodiment of the present application includes: a light emitting component 110, a fluid control component 120, a scattering detection component 130 and an image processing component 140. The fluid control component 120 includes an injection channel 121 and a flow chamber 122 connected to each other, and the flow chamber 122 is provided with a light-transmitting illumination area 123 through which the single particle to be detected passes.

[0035] The light emitting assembly 110 is generally composed of a light source 111 and related optical elements 112, wherein the light source 111 may be a helium-neon laser, a diode laser, a solid-state laser, a halogen lamp, a xenon lamp or a light-emitting diode lamp, etc., and the optical element 112 may be a filter, a lens or a power attenuator, etc. The light emitting assembly 110 is used to emit light of a specific wavelength into the illumination region 123 through which the single particle to be detected passes, wherein the selection of the specific wavelength is based on the scattering characteristics of the single particle to be detected and the desired detection target.

[0036] Please continue to refer to Figure 1 The fluid control component 120 includes an injection channel 121 and a flow chamber 122 that are connected to each other. The flow chamber 122 is provided with an illumination area 123 through which the light-transmitting single particle to be detected passes.

[0037] The injection channel 121 is usually composed of a long and thin pipe or channel. The injection channel 121 connects the source of the single particles to be detected (such as a blood sample) and the flow chamber 122, and is used to guide the single particles to be detected to enter the flow chamber 122 in a single and orderly manner.

[0038] The flow chamber 122 is generally a space with a certain width and height to accommodate the single particle to be tested and allow it to pass through the flow chamber 122 under flow conditions. The flow chamber 122 includes a light-transmitting illumination area 123, which can be made of a transparent material for transmitting the light generated by the light-emitting component 110. The flow chamber 122 is used to provide a controllable channel for the single particle to be tested. By controlling various parameters of the flow chamber 122, such as the flow rate and flow direction, it can be ensured that the single particle to be tested passes through the illumination area 123 in a uniform and controllable manner, and the single particle to be tested is irradiated by the light emitted by the light-emitting component 110 to generate forward scattered light and side scattered light.

[0039] The scattering detection component 130 is generally composed of a detector, an optical system 133, and a signal processing circuit, wherein the detector may be a photodiode or a photomultiplier tube, etc.; the optical system 133 may be composed of a lens, a filter, or other optical devices, and is used to focus and adjust the received scattered light signal so that it fits the receiving area of ​​the detector; the signal processing circuit is used to receive and process the electrical signal output by the detector, and to amplify, filter, sample, and convert the electrical signal. The scattering detection component 130 is used to convert the light signal scattered by the illumination area 123 into an electrical signal, wherein the intensity of the scattered light signal received by the scattering detection component 130 is related to the scattering characteristics (such as size, shape, or refractive index, etc.) of the single particle to be detected. By measuring and analyzing these signals, information about the single particle to be detected, such as particle concentration, size distribution, or aggregation state, etc., can be obtained.

[0040] The image processing component 140 is connected to the scattering detection component 130, and is used to receive, process and analyze the scattered light signal, and generate a forward scattered light-side information scatter plot containing the scattered points of the nucleated red blood cells. The image processing component 140 is generally composed of image acquisition, image enhancement, feature extraction, data analysis and image generation, wherein image acquisition is used to receive the electrical signal of the scattered light signal and convert it into digital image data for further processing; image enhancement is used to apply various algorithms to enhance the image quality, such as denoising, contrast enhancement or brightness adjustment; feature extraction is used to identify the existence and properties of nucleated red blood cells by calculating, analyzing and identifying specific patterns and features in the scattered light signal; data analysis and image generation are used to perform data analysis and processing based on the extracted feature information, generate a forward scattered light-side information scatter plot of the scattered light of the nucleated red blood cells, and display the position, size and concentration distribution of the nucleated red blood cells through the forward scattered light-side information scatter plot of the scattered light of the nucleated red blood cells, and further support the subsequent analysis and identification of the nucleated red blood cells.

[0041] In the above scheme, the nucleated red blood cell detection device 100 includes a light emitting component 110, a fluid control component 120, a scattering detection component 130 and an image processing component 140. The scattering detection component 130 detects the forward scattered light and the side scattered light formed after the single particle to be detected passes through the illumination area 123 in the fluid control component 120, and the image processing component 140 generates a forward scattered light-side information scatter plot based on the formed forward scattered light and side scattered light. The generated forward scattered light-side information scatter plot containing the nucleated red blood cell scatter points is analyzed to determine the nucleated red blood cell aggregation area adjacent to the white blood cell aggregation area in the scatter plot. By analyzing the number of nucleated red blood cells in the nucleated red blood cell aggregation area, information on whether the number of nucleated red blood cells is abnormal can be provided. In addition, when the nucleated red blood cells are detected by the nucleated red blood cell detection device 100, there is no need to add a fluorescent agent to the blood sample, the complexity and cost of the detection are low, and the information about the nucleated red blood cells can be provided quickly and easily.

[0042] Among them, the method of determining the nucleated red blood cell aggregation area adjacent to the white blood cell aggregation area in the scatter plot can be determined automatically by software, or it can be determined manually based on experience after the forward scattered light-side information scatter plot is displayed on the screen.

[0043] Please continue to refer to Figure 1The scattering detection assembly 130 includes a first scattering detection component 131 and a second scattering detection component 132. The first scattering detection component 131 is used to detect and obtain a forward scattered light signal, and the second scattering detection component 132 is used to detect and obtain a side scattered light signal. The first scattering detection component 131 may include optical devices such as a forward scattering detector, a lens, and a filter. The forward scattering detector is used to receive and convert the forward scattered light signal into an electrical signal, and other optical devices are used to adjust and focus the light beam to make it suitable for the receiving area of ​​the forward scattering detector. The second scattering detection component 132 may include optical devices such as a side scattering detector, a lens, and a filter. The side scattering detector is used to receive and convert the side scattered light signal into an electrical signal, and other optical devices are used to adjust and focus the light beam to make it suitable for the receiving area of ​​the side scattering detector.

[0044] In the above scheme, the forward scattered light signal and the side scattered light signal of the single particle to be detected can be detected by the first scattering detection element 131 and the second scattering detection element 132 respectively, and the information of scattered light in different directions of the single particle to be detected can be obtained, so as to obtain more comprehensive scattering characteristics of the single particle to be detected.

[0045] Please continue to refer to Figure 1 The nucleated red blood cell detection device 100 also includes a sampling component 150, which is used to input the processed blood sample into the fluidic component 120 for subsequent collection of forward scattered light and side scattered light information. Exemplarily, the sampling component 150 can be used to input the processed blood sample in one portion or two portions of the same amount into the fluidic component 120 at a certain time interval, wherein when two portions of the same amount are input into the fluidic component 120 one after another, the forward scattered light information of each cell is collected from the first portion of the blood sample, and the side scattered light information of each cell is collected from the second portion of the blood sample.

[0046] The above scheme, through the sampling component 150, inputs the processed blood sample in one portion or two portions of the same amount into the fluid control component 120 at a certain time interval, which can ensure that the forward scattered light information and the side scattered light information of each cell are collected in the fluid control component 120 respectively, so that the nucleated red blood cell detection device 100 can obtain more comprehensive and accurate cell scattering characteristics, thereby better detecting and analyzing the nucleated red blood cells.

[0047] Furthermore, the image processing component 140 in the nucleated red blood cell detection device 100 shown in the present application is further used to analyze the forward scattered light-side information scatter plot, and determine the area where the characteristic particle group does not appear in the forward scattered light-side information scatter plot of the normal blood sample but the characteristic particle group appears in the forward scattered light-side information scatter plot of the single particle to be tested as the nucleated red blood cell aggregation area, and count the nucleated red blood cells in the nucleated red blood cell aggregation area to obtain the sample count, and determine whether the number of the sample count is within a predetermined range.

[0048] Exemplarily, the image processing component 140 can obtain the forward scattered light signal and the side scattered light signal of the normal blood sample through the scattering detection component 130, generate the forward scattered light-side information scatter diagram of the normal blood sample, and by comparing the forward scattered light-side information scatter diagram of the normal blood sample with the forward scattered light-side information scatter diagram of the sample to be tested, the image processing component 140 can mark the area where the characteristic particle group appears in the sample to be tested and the area where the characteristic particle group does not appear in the normal blood sample map as the nucleated red blood cell aggregation area. In the nucleated red blood cell aggregation area, the image processing component 140 can use image processing algorithms and analysis techniques to count each nucleated red blood cell. Exemplarily, the image processing component 140 can identify and count based on the characteristics of the nucleated red blood cells, such as shape or size, and determine whether the number of sample counts is within a predetermined range. If the counting result meets the predetermined range, it means that the number of nucleated red blood cells in the sample is normal. If the counting result exceeds the predetermined range, it may indicate that the nucleated red blood cells are aggregated too much or too little, and there is some abnormality, which requires further analysis and processing.

[0049] In the above scheme, the nucleated red blood cell detection device 100 can analyze the forward scattered light-side information scatter plot of the normal blood sample and the forward scattered light-side information scatter plot of the sample to be tested through the image processing component 140, and locate and determine the area where the nucleated red blood cells may be aggregated, use image processing algorithms and analysis techniques to count each nucleated red blood cell in the area where the nucleated red blood cells are aggregated, and evaluate the number of nucleated red blood cells in the sample according to a predetermined range.

[0050] Please continue to refer to Figure 1 The nucleated red blood cell detection device 100 also includes an alarm component 160, which is connected to the image processing component 140 and is used to perform alarm processing when the number of sample counts is not within a predetermined range and the number of nucleated red blood cells in the blood sample is determined to be abnormal.

[0051] Exemplarily, when the image processing component 140 completes the counting of nucleated red blood cells and determines that the counting result exceeds the predetermined range, and the number of nucleated red blood cells in the sample is abnormal, the alarm component 160 will trigger an alarm signal, wherein the specific alarm method may vary according to the design and requirements of the device, including but not limited to alarms in the form of sound, light signals, or display information. The nucleated red blood cell detection device 100 can timely detect the abnormal number of nucleated red blood cells in the sample through the alarm component 160, and remind relevant personnel to handle it, so as to ensure the accuracy and reliability of the results.

[0052] See also Figure 2 , Figure 2 The present application shows a schematic flow chart of an exemplary embodiment of a method for analyzing nucleated red blood cells. Specifically, the method may include the following steps:

[0053] Step S110: Acquire forward scattered light information and side scattered light information of cells in the blood sample when the blood sample passes through the detection area of ​​the detection device.

[0054] Forward scattered light information can be information about cell size, cell shape, etc. based on forward scattered light analysis. Among them, forward scattered light can refer to light that is incident into a sample from one direction and then scattered out in the same direction as the incident direction. For example, in the detection process of nucleated red blood cells, a blood sample passes through the detection area of ​​a detection device. When the light generated by the detection device encounters nucleated red blood cells, part of the light is scattered in the forward direction, and the detection device can obtain forward scattered light information of cells in the blood sample. Forward scattered light information provides information about the size and shape of nucleated red blood cells. The detection device can infer the morphological characteristics of nucleated red blood cells, such as size or shape, by measuring and analyzing the intensity and distribution of forward scattered light.

[0055] Side scattered light information can be information about the internal structure and complexity of cells based on side scattered light analysis. Side scattered light refers to light that is incident into a sample from one direction and then scattered in a direction perpendicular to the incident direction. For example, during the detection of nucleated red blood cells, a blood sample passes through the detection area of ​​a detection device. When the light generated by the detection device encounters nucleated red blood cells, part of the light is scattered in a side direction, and the detection device can obtain side scattered light information of cells in the blood sample. Side scattered light information provides information about the internal structure and complexity of nucleated red blood cells. The detection device can infer the internal structure and complexity of nucleated red blood cells by measuring and analyzing the intensity and distribution of side scattered light.

[0056] During the detection of nucleated red blood cells, the detection equipment simultaneously measures and analyzes the information of forward scattered light and side scattered light, and can obtain more comprehensive data on the characteristics and properties of nucleated red blood cells. These data can be used to identify and analyze the nucleated red blood cells in the sample and help with related diagnosis and research work.

[0057] Step S120: generating a forward scattered light-side scatter plot including nucleated red blood cell scatter points according to the forward scattered light information and the side scattered light information.

[0058] The forward scattered light-side scatter plot can show the distribution of scattered light in the sample and is used to analyze the aggregation area of ​​nucleated red blood cells. In the forward scattered light-side scatter plot, the horizontal axis can represent the side scattered light and the vertical axis can represent the forward scattered light.

[0059] Exemplarily, during the detection of nucleated red blood cells, the detection device can record the scattered light signals of nucleated red blood cells through lasers, light scatterers or other optical devices, perform data processing and analysis such as conversion, filtering or normalization through an image processing system or related software, and plot each data point on a scatter plot based on the processed data, using forward scattered light and side scattered light as coordinate axes, wherein each data point represents a scatter signal of a measurement point, and its position is determined by the values ​​of forward scattered light and side scattered light. For normal blood samples, the forward scattered light-side scatter plot may show a uniform distribution of scattered light spots, while in samples with nucleated red blood cell aggregation, there may be areas of characteristic particle groups, which may show some obvious clustering or concentration in the forward scattered light-side scatter plot.

[0060] Step S130: obtaining a nucleated red blood cell aggregation region of a forward scattered light-side scatter plot according to the forward scattered light-side scatter plot containing the nucleated red blood cell scatter points.

[0061] Nucleated red blood cell aggregation refers to the area where red blood cells are aggregated together in blood samples or observed under a microscope. This aggregation phenomenon may be caused by various factors, including disease, inflammation, changes in blood rheology, etc.

[0062] Illustratively, during the detection of nucleated red blood cells, the presence of nucleated red blood cell aggregation areas can be observed through instruments such as microscopes or flow cytometry, and the detection equipment can analyze the image of the blood sample through image processing software or algorithms, wherein the process of analyzing the image of the blood sample may involve computer vision techniques such as threshold processing, segmentation or edge detection to extract the location and morphological information of the nucleated red blood cell aggregation area from the image data.

[0063] Step S140: Count the nucleated red blood cells in the nucleated red blood cell aggregation area to obtain a sample count, and determine whether the number of the sample count is within a predetermined range.

[0064] By counting the nucleated red blood cells in the nucleated red blood cell aggregation area, the detection device can obtain the number of nucleated red blood cells in the sample, and by comparing the counting result with the predetermined range, the detection device can determine whether the sample count is within the predetermined range. If the counting result is within the predetermined range, it means that the number of nucleated red blood cells in the sample is normal. If the counting result exceeds the predetermined range, it may indicate that the nucleated red blood cells are aggregated too much or too little.

[0065] Step S150: In response to the number of sample counts not being within a predetermined range, it is determined that the number of nucleated red blood cells in the blood sample is abnormal, and an alarm process is performed.

[0066] If the number of sample counts is not within the predetermined range, the detection equipment can determine that the number of nucleated red blood cells in the blood sample is abnormal and perform an alarm process. The specific alarm processing method may be to alert the operator through sound or flashing, so as to attract the operator's attention and take necessary measures in time; it may output alarm information, such as text or graphic prompts, to notify relevant staff of the abnormal number of nucleated red blood cells. It should be noted that the specific alarm processing method should be determined according to the actual application, equipment and system design.

[0067] In the above scheme, the detection device can generate a forward scattered light-side scatter plot containing nucleated red blood cell scatter points by obtaining the forward scattered light information and side scattered light information of the cells in the blood sample when the blood sample passes through the detection area of ​​the detection device, and determine the nucleated red blood cell aggregation area according to the forward scattered light-side scatter plot, count the nucleated red blood cells in the nucleated red blood cell aggregation area to obtain the sample count, and perform alarm processing when the number of sample counts is not within a predetermined range. This scheme can realize the detection of nucleated red blood cells without using a fluorescent agent, and the complexity and cost of the detection are low, and it can quickly and easily provide information about nucleated red blood cells.

[0068] See also Figure 3 , Figure 3 yes Figure 2 The flowchart of an exemplary embodiment before step S110 in the method for analyzing nucleated red blood cells shown in the figure may include the following steps:

[0069] S210: Obtaining an initial blood sample containing white blood cells.

[0070] The main function of white blood cells is immunity and defense. White blood cells in blood samples can provide other important information, such as determining the degree of inflammation or infection. In the detection of nucleated red blood cells, the initial blood sample containing white blood cells mainly serves to provide a reference for the overall blood cell composition and blood status.

[0071] S220: Mixing the initial blood sample with a predetermined reagent in a certain ratio to form a blood sample treated with the predetermined reagent, wherein the predetermined reagent can dissolve red blood cells in the initial blood sample.

[0072] The initial blood sample is mixed with a predetermined reagent in a certain proportion to form a blood sample treated with the predetermined reagent, wherein the predetermined reagent may include a lytic agent for dissolving or destroying red blood cells. The processed blood sample is usually called a whole blood smear sample or a blood cell count sample, which breaks up the red blood cells and releases cell components such as white blood cells, platelets, and a small amount of cell fragments. The selection of the predetermined reagent usually varies according to the specific experimental or analytical requirements. The predetermined reagent of this scheme can dissolve red blood cells and retain the characteristics and integrity of other cell components to the greatest extent, thereby providing accurate analysis results.

[0073] The above scheme obtains an initial blood sample containing white blood cells, mixes the initial blood sample with a predetermined reagent in a certain proportion, and forms a blood sample treated with the predetermined reagent, wherein the predetermined reagent can dissolve the red blood cells in the initial blood sample, so that the cell components can be more conveniently analyzed, counted or observed, thereby improving the degree of cell visualization, and the addition of the predetermined reagent helps to maintain the integrity and stability of the cell components, thereby ensuring the accuracy and reliability of subsequent experiments or analyses.

[0074] Furthermore, in the method for analyzing nucleated red blood cells shown in the present application, step S120: generating a forward scattered light-side scatter plot containing nucleated red blood cell scatter points according to the forward scattered light information and the side scattered light information, the method further includes:

[0075] According to the forward scattered light information and the side scattered light information, the white blood cells in the blood sample are divided into at least four categories: lymphocyte group, monocyte group, eosinophil group, and neutrophil group.

[0076] Exemplarily, in the detection process of nucleated red blood cells, forward scattered light usually represents the size of the cell, while side scattered light usually represents the complexity or particle content of the cell. The forward scattered light and side scattered light signals of each cell can be recorded by passing the blood sample through the nucleated red blood cell detection device in sequence, thereby generating a forward scattered light-side scattered light scatter plot. According to the forward scattered light-side scattered light scatter plot, using the corresponding threshold and gating strategy, the white blood cells in the blood sample can be divided into at least four categories: lymphocyte group, monocyte group, eosinophil group, and neutrophil group, among which small and low-scattering cells in the forward scattered light-side scattered light map can be defined as lymphocyte group; large and high-scattering cells in the forward scattered light-side scattered light map can be defined as monocyte group; highly scattered cells in the forward scattered light-side scattered light map can be defined as eosinophil group; medium-sized and medium-scattering cells in the forward scattered light-side scattered light map can be defined as neutrophil group.

[0077] The above scheme can separate red blood cells and white blood cells, determine the ratio of white blood cell types, and provide more comprehensive cell information by dividing the white blood cells in the blood sample into four major categories: lymphocyte group, monocyte group, eosinophil group and neutrophil group, to assist in the subsequent detection and research of nucleated red blood cells.

[0078] See also Figure 4 , Figure 4 yes Figure 2 The flowchart of an exemplary embodiment of step S130 in the method for analyzing nucleated red blood cells is shown. Based on the above embodiment, step S130 of obtaining the nucleated red blood cell aggregation area of ​​the forward scattered light-side scatter plot according to the forward scattered light-side scatter plot containing the nucleated red blood cell scatter points also includes:

[0079] S310: Acquire position data of a lymphocyte group and a blood shadow area, wherein the lymphocyte group and the blood shadow area are acquired through image processing or computer vision.

[0080] Please refer to Figure 5 , Figure 5 : is a schematic diagram of obtaining the nucleated red blood cell aggregation area shown in the present application. The blood shadow area may refer to an area formed due to the characteristics of blood cells scattering light. The blood shadow area usually refers to a scattered area of ​​a cell group related to white blood cells. The blood shadow area may have different characteristics, such as size, shape or position distribution, which can be used to determine the classification and number of white blood cells.

[0081] Exemplarily, during the detection of nucleated red blood cells, the nucleated red blood cell detection device can use an appropriate microscope or other imaging device to obtain an image of a blood sample, and pre-process the image, including denoising, contrast enhancement, brightness adjustment, etc., to improve the recognizability of the cells, and then use image processing or computer vision to segment the lymphocyte group and the blood shadow area, wherein the identification and segmentation can be based on the morphological characteristics, color information or other characteristics of the cells. The nucleated red blood cell detection device can describe the position of the lymphocyte group and the blood shadow area by calculating the center coordinates of the cell group, the boundary coordinates of the outer contour, or other shape features, and by analyzing the acquired position data, the characteristics and statistical information of the lymphocyte group and the blood shadow area, such as quantity, size, distribution, etc., can be obtained.

[0082] S320: Determine the location of the nucleated red blood cell aggregation area based on the location data of the lymphocyte group and the blood shadow area.

[0083] The nucleated red blood cell detection equipment can match and associate the position data of the lymphocyte group and the blood shadow area with the position data of the nucleated red blood cells, and based on the matching and association results, further analyze and calculate the position of the aggregation area of ​​the nucleated red blood cells to determine the degree and position range of the nucleated red blood cell aggregation, wherein statistical methods, clustering algorithms or other related technologies can be used.

[0084] In the above scheme, the nucleated red blood cell detection device can distinguish nucleated red blood cells from other types of cells, such as lymphocytes or monocytes, by determining the location of the nucleated red blood cell aggregation area, which helps to accurately calculate the number of nucleated red blood cells and provide quantitative information about the composition of blood cells.

[0085] It should be further explained that the executor of the nucleated red blood cell analysis method may be a nucleated red blood cell analysis device, for example, the nucleated red blood cell analysis device may be a terminal device or a server or other processing device, wherein the terminal device may be a user equipment (UE), a computer, a mobile device, a user terminal, a terminal, a cellular phone, a cordless phone, a personal digital assistant (PDA), a handheld device, a computing device, a vehicle-mounted device or a wearable device, etc.

[0086] See also Figure 6 , Figure 6 FIG. 1 is a schematic diagram of the structure of an embodiment of a nucleated red blood cell analysis device shown in the present application. Figure 6As shown, the exemplary nucleated red blood cell analysis device 400 includes: a first acquisition module 410, a scatter plot generation module 420, a second acquisition module 430, a counting module 440 and an alarm module 450, specifically: the first acquisition module 410 is used to obtain the forward scattered light information and the side scattered light information of the cells in the blood sample when the blood sample passes through the detection area of ​​the detection device; the scatter plot generation module 420 is used to generate a forward scattered light-side scatter plot containing nucleated red blood cell scatter points according to the forward scattered light information and the side scattered light information; the second acquisition module 430 is used to obtain the nucleated red blood cell aggregation area of ​​the forward scattered light-side scatter plot according to the forward scattered light-side scatter plot containing nucleated red blood cell scatter points; the counting module 440 is used to count the nucleated red blood cells in the nucleated red blood cell aggregation area to obtain a sample count, and determine whether the number of the sample count is within a predetermined range; the alarm module 450 is used to determine that the number of nucleated red blood cells in the blood sample is abnormal in response to the number of the sample count not being within the predetermined range, and perform alarm processing.

[0087] In the above scheme, the detection device can generate a forward scattered light-side scattered light diagram containing nucleated red blood cell scatter points by obtaining the forward scattered light information and side scattered light information of the cells in the blood sample when the blood sample passes through the detection area of ​​the detection device, locate the nucleated red blood cell aggregation area according to the forward scattered light-side scattered light diagram, count the nucleated red blood cells in the nucleated red blood cell aggregation area to obtain the sample count, and perform alarm processing when the number of sample counts is not within a predetermined range. In addition, the detection device can realize the detection of nucleated red blood cells without using a fluorescent agent, and the detection complexity and cost are low, and the information about nucleated red blood cells can be provided quickly and easily.

[0088] The functions of each module can be found in the embodiment of the analysis method of nucleated red blood cells, which will not be described in detail here.

[0089] See also Figure 7 , Figure 7 The computer storage medium 500 stores program instructions 501 that can be executed by a processor, and the program instructions 501 are used to implement the steps in any of the above-mentioned methods for analyzing nuclear red blood cells.

[0090] In the above scheme, the detection device can generate a forward scattered light-side scattered light diagram containing nucleated red blood cell scatter points by obtaining the forward scattered light information and side scattered light information of the cells in the blood sample when the blood sample passes through the detection area of ​​the detection device, locate the nucleated red blood cell aggregation area according to the forward scattered light-side scattered light diagram, count the nucleated red blood cells in the nucleated red blood cell aggregation area to obtain the sample count, and perform alarm processing when the number of sample counts is not within a predetermined range. In addition, the detection device can realize the detection of nucleated red blood cells without using a fluorescent agent, and the detection complexity and cost are low, and the information about nucleated red blood cells can be provided quickly and easily.

[0091] The above description of various embodiments tends to emphasize the differences between the various embodiments. The same or similar aspects can be referenced to each other, and for the sake of brevity, they will not be repeated herein.

[0092] In the several embodiments provided in the present application, it should be understood that the disclosed methods and devices can be implemented in other ways. For example, the device implementation described above is only schematic. For example, the division of modules or units is only a logical function division. There may be other division methods in actual implementation, such as units or components can be combined or integrated into another system, or some features can be ignored or not executed. Another point is that the mutual coupling or direct coupling or communication connection shown or discussed can be through some interfaces, and the indirect coupling or communication connection of devices or units can be electrical, mechanical or other forms.

[0093] In addition, each functional unit in each embodiment of the present application can be integrated into a processing unit, or each unit can exist physically separately, or two or more units can be integrated into one unit. The above-mentioned integrated unit can be implemented in the form of hardware or in the form of a software functional unit. If the integrated unit is implemented in the form of a software functional unit and sold or used as an independent product, it can be stored in a computer storage medium. Based on this understanding, the technical solution of the present application is essentially or the part that contributes to the prior art or all or part of the technical solution can be embodied in the form of a software product, and the computer software product is stored in a storage medium, including several instructions to enable a computer device (which can be a personal computer, a server, or a network device, etc.) or a processor (processor) to perform all or part of the steps of each implementation method of the present application. The aforementioned storage medium includes: U disk, mobile hard disk, read-only memory (ROM, Read-Only Memory), random access memory (RAM, Random Access Memory), disk or optical disk and other media that can store program code.

Claims

1. A nucleated red blood cell detection device, characterized in that: The device comprises: A light emitting component for emitting light of a predetermined wavelength; A fluidic control component, the fluidic control component comprises an injection channel and a flow chamber connected to each other, wherein the flow chamber is provided with an illumination area through which a light-transmitting single particle to be detected passes; A scattering detection component, used to detect forward scattered light and side scattered light formed after the single particle to be detected passes through the illumination area, thereby obtaining a forward scattered light signal and a side scattered light signal; An image processing component, connected to the scatter detection component, for generating a forward scattered light-side information scatter plot containing nucleated red blood cell scatter points according to the forward scattered light signal and the side scattered light signal; Wherein, the preset area adjacent to the white blood cell aggregation area in the forward scattered light-side information scattergram is defined as the nucleated red blood cell aggregation area.

2. The nucleated red blood cell detection device according to claim 1, characterized in that: The scattering detection assembly includes a first scattering detection component and a second scattering detection component, wherein the first scattering detection component is used to detect and obtain the forward scattered light signal; The second scattering detection element is used to detect and obtain the side scattered light signal.

3. The nucleated red blood cell detection device according to claim 1, characterized in that: The nucleated red blood cell detection device also includes a sampling component, which is used to input the processed blood sample in one portion or two portions of equal size into the fluidic control component successively, wherein, in the process of inputting the two portions of equal size into the fluidic control component successively, the forward scattered light information of each cell is collected from the first blood sample, and the side scattered light information of each cell is collected from the second blood sample.

4. The nucleated red blood cell detection device according to claim 1, characterized in that: The image processing component is further used to analyze the forward scattered light-side information scatter plot, and determine the area where the characteristic particle group does not appear in the forward scattered light-side information scatter plot of the normal blood sample, but the characteristic particle group appears in the forward scattered light-side information scatter plot of the single particle to be tested as the nucleated red blood cell aggregation area.

5. The nucleated red blood cell detection device according to claim 4, characterized in that: The image processing component is used to count the nucleated red blood cells in the nucleated red blood cell aggregation area to obtain a sample count, and to determine whether the number of the sample count is within a predetermined range.

6. The nucleated red blood cell detection device according to claim 5, characterized in that: The nucleated red blood cell detection device includes an alarm component connected to the image processing component, which is used to perform alarm processing when the number of the sample count is not within a predetermined range and the number of nucleated red blood cells in the blood sample is determined to be abnormal.

7. A method for analyzing nucleated red blood cells, characterized in that: The method comprises: Acquire forward scattered light information and side scattered light information of cells in the blood sample when the blood sample passes through the detection area of ​​the detection device; Generating a forward scattered light-side scatter plot containing nucleated red blood cell scatter points according to the forward scattered light information and the side scattered light information; Obtaining a nucleated red blood cell aggregation region of the forward scattered light-side scatter plot according to the forward scattered light-side scatter plot containing nucleated red blood cell scatter points; Counting the nucleated red blood cells in the nucleated red blood cell aggregation area to obtain a sample count, and determining whether the number of the sample count is within a predetermined range; In response to the number of the sample count not being within a predetermined range, it is determined that the number of nucleated red blood cells in the blood sample is abnormal, and an alarm process is performed.

8. The method for analyzing nucleated red blood cells according to claim 7, characterized in that: Before the step of obtaining forward scattered light information and side scattered light information of cells in the blood sample when the blood sample passes through the cytometer detection area, the method further includes: Obtain an initial blood sample containing white blood cells; The initial blood sample is mixed with a predetermined reagent in a certain ratio to form the blood sample treated with the predetermined reagent, wherein the predetermined reagent can dissolve red blood cells in the initial blood sample.

9. The method for analyzing nucleated red blood cells according to claim 7, characterized in that: The step of generating a forward scattered light-side scatter plot containing nucleated red blood cell scatter points according to the forward scattered light information and the side scattered light information, the method further comprises: According to the forward scattered light information and the side scattered light information, the white blood cells in the blood sample are divided into at least four categories: lymphocyte group, monocyte group, eosinophil group, and neutrophil group.

10. The method for analyzing nucleated red blood cells according to claim 7, characterized in that: The step of obtaining the nucleated red blood cell aggregation area of ​​the forward scattered light-side scatter plot according to the forward scattered light-side scatter plot containing the nucleated red blood cell scatter points, the method further comprises: Acquiring position data of a lymphocyte group and a blood ghost area, wherein the lymphocyte group and the blood ghost area are acquired by image processing or computer vision; The position of the nucleated red blood cell aggregation area is determined based on the position data of the lymphocyte group and the blood ghost area.

11. A device for analyzing nucleated red blood cells, characterized in that: The device comprises: A first acquisition module is used to acquire forward scattered light information and side scattered light information of cells in the blood sample when the blood sample passes through the detection area of ​​the detection device; A scatter plot generation module, used for generating a forward scattered light-side scatter plot containing nucleated red blood cell scatter points according to the forward scattered light information and the side scattered light information; A second acquisition module is used to acquire the nucleated red blood cell aggregation area of ​​the forward scattered light-side scatter plot according to the forward scattered light-side scatter plot containing the nucleated red blood cell scatter points; A counting module, used for counting the nucleated red blood cells in the nucleated red blood cell aggregation area to obtain a sample count, and determining whether the number of the sample count is within a predetermined range; The alarm module is used to determine that the number of nucleated red blood cells in the blood sample is abnormal in response to the number of the sample count not being within a predetermined range, and to perform an alarm process.

12. A computer-readable storage medium having program instructions stored thereon, characterized in that: When the program instructions are executed by a processor, the method for analyzing nucleated red blood cells according to any one of claims 7 to 10 is implemented.

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