A composite foliar barrier and its application in reducing cadmium accumulation in brown rice

Abstract

The invention discloses a composite foliar barrier and application thereof in reducing cadmium accumulation in brown rice. The composite foliar barrier comprises: NaHS concentration of 10-20 μmol / L; melatonin concentration of 20-30 μmol / L; taurine concentration of 0.1-0.5 g / L; surfactant concentration of 0.1-0.3 g / L; and the remainder of water. The composite foliar barrier is sprayed in the tillering stage, heading stage and filling stage of rice, and the spraying amount per mu is 30-50 kg each time. The invention utilizes the synergistic effect of NaHS, melatonin and taurine, adopts surfactant to increase the spreading area, and jointly greatly improves the cadmium reduction effect of brown rice, and the cadmium reduction efficiency can reach more than 85%. The foliar barrier of the invention has simple components, is easy to operate, and has outstanding effect.

Description

Technical Field

[0001] The invention relates to the technical field of agriculture, and in particular to a composite foliar barrier and control agent and application thereof in reducing cadmium accumulation in brown rice. Background Art

[0002] Existing foliar control agents mostly use a single ingredient, such as nutrients, humic acid or amino acids. Although these control agents can reduce the cadmium content in leaves, they cannot prevent the bioaccumulation of cadmium, resulting in a large amount of cadmium in the stems and leaves. In addition, during the spraying process, these control agents often cannot be effectively adsorbed on the wax layer on the leaves, resulting in the loss of the agent, low utilization efficiency, and low actual cadmium reduction effect in the field.

[0003] Therefore, there is an urgent need to develop a new type of composite foliar barrier agent, which can not only effectively reduce the cadmium content in rice brown rice, but also improve the adsorption efficiency and utilization efficiency of the agent without negatively affecting crop growth and soil structure. Summary of the invention

[0004] The invention aims to overcome the disadvantages of the prior art and provides a composite foliar barrier and application thereof in reducing cadmium accumulation in brown rice. The composite foliar barrier has the following characteristics: NaHS concentration is 10-20 μmol / L; melatonin concentration is 20-30 μmol / L; taurine concentration is 0.1-0.5 g / L; surfactant concentration is 0.1-0.3 g / L; the rest is water; the composite foliar barrier is sprayed in the tillering stage, heading stage and filling stage of rice, and the spraying amount per mu is 30-50 kg each time; the invention utilizes the synergistic effect of NaHS, melatonin and taurine, adopts surfactant to increase the spreading area, and jointly greatly improves the cadmium reduction effect of brown rice, and the cadmium reduction efficiency can reach more than 85%; the foliar barrier of the invention has simple ingredients, is easy to operate and has outstanding effect.

[0005] In order to achieve the above technical effects, the following technical solutions are adopted:

[0006] A composite leaf barrier control agent, the component formula is:

[0007] The concentration of NaHS is 10-20 μmol / L; the concentration of melatonin is 20-30 μmol / L; the concentration of taurine is 0.1-0.5 g / L; the concentration of surfactant is 0.1-0.3 g / L; and the balance is water.

[0008] Furthermore, in the composite leaf barrier agent, the surfactant is Tween 80 or OP-10.

[0009] Furthermore, the composition formula of the composite leaf surface barrier agent is:

[0010] NaHS: 20 μmol / L; melatonin: 30 μmol / L; taurine: 0.3 g / L; surfactant concentration is 0.2 g / L; the balance is water.

[0011] Furthermore, the preparation method of the composite leaf surface barrier agent is:

[0012] Weigh NaHS, melatonin, taurine, surfactant and water according to the concentration requirements and preparation amount; directly put NaHS into water to dissolve, stir evenly, then add melatonin, taurine and surfactant in sequence, stir and dissolve evenly to obtain the composite foliar barrier agent, which should be prepared before use and should not be stored for more than 6 hours.

[0013] Furthermore, the application method is:

[0014] The composite foliar barrier agent is sprayed at the tillering stage, heading stage and grain filling stage of rice, with a spraying amount of 30-50 kg per mu each time.

[0015] Furthermore, the spraying parts are the leaf surfaces and backs of rice leaves.

[0016] Furthermore, the composite foliar barrier agent is sprayed before 10 am and after 4 pm on sunny or cloudy days.

[0017] Furthermore, the suitable temperature range for spraying the composite foliar barrier agent is 15-30°C.

[0018] Furthermore, the composite foliar barrier is used to reduce cadmium accumulation in brown rice.

[0019] The beneficial effects of the present invention are:

[0020] The invention discloses a composite foliar barrier and application thereof in reducing cadmium accumulation in brown rice. The composite foliar barrier comprises: NaHS concentration of 10-20 μmol / L; melatonin concentration of 20-30 μmol / L; taurine concentration of 0.1-0.5 g / L; surfactant concentration of 0.1-0.3 g / L; and the rest is water. The composite foliar barrier is sprayed in the tillering stage, heading stage and filling stage of rice, and the spraying amount per mu is 30-50 kg each time. The invention utilizes the synergistic effect of NaHS, melatonin and taurine, adopts surfactant to increase the spreading area, and jointly greatly improves the cadmium reduction effect of brown rice, and the cadmium reduction efficiency can reach more than 85%. The foliar barrier of the invention has simple ingredients, is easy to operate and has outstanding effect. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0022] It should be noted that the following detailed descriptions are exemplary and are intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs.

[0023] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit exemplary embodiments according to the present invention. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprising" and / or "including" are used in this specification, it indicates the presence of features, steps, operations and / or combinations thereof.

[0024] Embodiment 1:

[0025] The concentration of NaHS is 10 μmol / L; the concentration of melatonin is 20 μmol / L; the concentration of taurine is 0.1 g / L; the concentration of surfactant is 0.1 g / L; and the balance is water.

[0026] Taking 100L of foliar barrier agent as an example, NaHS: 0.056g; melatonin: 0.465g; taurine: 10g; surfactant Tween 80: 10g; the rest is water.

[0027] According to the above concentrations and the required preparation amounts, NaHS, melatonin, taurine, surfactant and water were weighed; NaHS was directly put into water to dissolve, and after stirring evenly, melatonin, taurine and surfactant were added in sequence, and stirred to dissolve evenly to obtain the composite foliar barrier agent 1.

[0028] Embodiment 2:

[0029] The concentration of NaHS is 20 μmol / L; the concentration of melatonin is 30 μmol / L; the concentration of taurine is 0.5 g / L; the concentration of surfactant is 0.3 g / L; and the balance is water.

[0030] Taking 100L of foliar barrier agent as an example, NaHS: 0.112g; melatonin: 0.698g; taurine: 50g; surfactant Tween 80: 30g; the rest is water.

[0031] Weigh NaHS, melatonin, taurine, surfactant and water according to the above concentration and required preparation amount; directly put NaHS into water to dissolve, stir evenly, then add melatonin, taurine and surfactant in sequence, stir and dissolve evenly to obtain composite foliar barrier agent 2.

[0032] Embodiment 3:

[0033] NaHS: 20 μmol / L; melatonin: 30 μmol / L; taurine: 0.3 g / L; surfactant concentration is 0.2 g / L; the balance is water.

[0034] Taking 100L of foliar barrier agent as an example, NaHS: 0.112g; melatonin: 0.698g; taurine: 30g; surfactant Tween 80: 20g; the rest is water.

[0035] According to the above concentrations and the required preparation amounts, weigh NaHS, melatonin, taurine, surfactant and water; directly put NaHS into water to dissolve, stir evenly, then add melatonin, taurine and surfactant in sequence, stir and dissolve evenly to obtain composite foliar barrier agent 3.

[0036] All comparative examples are based on Example 3.

[0037] Comparative Example 1:

[0038] Taking 100L of foliar barrier agent as an example, melatonin: 0.698g + 0.112g; taurine: 30g; surfactant Tween 80: 20g; the rest is water.

[0039] According to the above concentrations and the required preparation amounts, melatonin, taurine, surfactant and water are weighed; melatonin is directly put into water to dissolve, and after stirring evenly, taurine and surfactant are added in sequence, and stirred to dissolve evenly to obtain composite foliar barrier agent 4.

[0040] Comparative Example 2:

[0041] Taking 100L of foliar barrier agent as an example, NaHS: 0.112g + 0.698g; taurine: 30g; surfactant Tween 80: 20g; the rest is water.

[0042] According to the above concentration and the required preparation amount, weigh NaHS, taurine, surfactant and water; directly put NaHS into water to dissolve, stir evenly, then add taurine and surfactant in sequence, stir and dissolve evenly to obtain composite foliar barrier agent 5.

[0043] Comparative Example 3:

[0044] Taking 100L foliar barrier agent as an example, NaHS: 0.112g+30g; melatonin: 0.698g; surfactant Tween 80: 20g; the rest is water.

[0045] According to the above concentration and the required preparation amount, weigh NaHS, melatonin, surfactant and water; directly put NaHS into water to dissolve, stir evenly, then add melatonin and surfactant in sequence, stir and dissolve evenly to obtain composite foliar barrier agent 6.

[0046] Comparative Example 4:

[0047] Taking 100L of foliar barrier agent as an example, taurine: 0.112g + 0.698g + 30g; surfactant Tween 80: 20g; the rest is water.

[0048] Taurine, surfactant and water are weighed according to the above concentration and the required preparation amount; taurine is directly put into water to dissolve, and after stirring evenly, surfactants are added in sequence, and stirred to dissolve evenly to obtain composite foliar barrier agent 7.

[0049] Comparative Example 5

[0050] Taking 100L of foliar barrier agent as an example, melatonin: 0.112g + 0.698g + 30g; surfactant Tween 80: 20g; the rest is water.

[0051] According to the above concentration and the required preparation amount, melatonin, surfactant and water are weighed; melatonin is directly put into water to dissolve, and after stirring evenly, the surfactant is added and stirred to dissolve evenly to obtain the composite foliar barrier agent 8.

[0052] Comparative Example 6:

[0053] Taking 100L of foliar barrier agent as an example, NaHS: 0.112g + 0.698g + 30g; surfactant Tween 80: 20g; the rest is water.

[0054] According to the above concentration and the required preparation amount, weigh NaHS, surfactant and water; directly put NaHS into water to dissolve, stir and dissolve evenly to obtain composite foliar barrier agent 9.

[0055] Comparative Example 7:

[0056] Taking 100L of foliar control agent as an example, the surfactant Tween 80:20g; the rest is water.

[0057] According to the above concentration and the required preparation amount, weigh the surfactant and water; directly put the surfactant into the water to dissolve, stir and dissolve evenly to obtain the composite foliar barrier agent 10.

[0058] The composite leaf surface control agents in Examples 1-3 and Comparative Examples 1-7 are evaluated below:

[0059] Spraying effect verification test: Field plot tests were conducted on the composite foliar barrier agents in Examples 1-3 and Comparative Examples 1-7. The test process is as follows:

[0060] Specifically:

[0061] Test location: Huotian Village, Shuixi Town, Yushui District, Xinyu City

[0062] Experimental field: total cadmium content in soil is 0.49 mg / kg; pH is 5.15, effective cadmium content is 0.30 mg / kg, and rice variety is the local main variety Xing'an Xiangzhan.

[0063] Trial time: 2024 late rice season

[0064] Experimental setup: The above examples 1-3 and comparative examples 1-7 were treated in a single treatment area, and the entire field was divided into 44 plots, each with an area of ​​20 m 2 , leaving 25cm space between the plots, and no ridges. All plots are in the same field, and the growth environment, soil fertility and other conditions are completely consistent. Every 4 plots are a treatment area (4 repeated experiments), with a total of 11 treatment areas, treatment area 1 is a blank control group CK (spraying an equal amount of clean water blank control group), and treatment areas 2-11 are respectively the composite foliar barrier agents in Examples 1-3 and Comparative Examples 1-7;

[0065] The application amount of each composite foliar barrier agent is 40kg / mu; the spraying site is the rice leaf surface and leaf back; the spraying time of the composite foliar barrier agent is before 10 am and after 4 pm on a sunny or cloudy day. The suitable temperature range for spraying is 25℃.

[0066] The composite foliar barrier control agents were sprayed on the rice in each treatment area using a back-type sprayer at the tillering stage, heading stage and grain filling stage of the rice.

[0067] Other field water and fertilizer management methods and pest and disease control methods remain consistent with traditional methods. After the rice matures (early November), the rice is harvested and the yield is measured in each plot. The rice grains are collected and threshed; and the cadmium content in the rice is measured.

[0068] The method for determining the cadmium content of brown rice is as follows: take 0.1g brown rice powder sample, add 5mL nitric acid and 3.0mL hydrogen peroxide solution, cover and cold digest overnight, digest for 2h using a graphite digester and dilute to 25mL, and analyze and determine the cadmium concentration in the digestion solution using an inductively coupled plasma mass spectrometer. The test results are shown in Table 1:

[0069] Table 1 Differences in rice yield and cadmium content in brown rice among treatments

[0070]

[0071] Note 1: Except for the different treatment methods, the other conventional cultivation methods of each group in the table are the same.

[0072] Note 2: According to the requirements of "GB 2762-2022 National Food Safety Standard Limits of Contaminants in Food", the cadmium content of qualified rice is less than or equal to 0.2 mg / kg.

[0073] According to the experimental results of the above embodiments, the present invention utilizes the synergistic effect of NaHS, melatonin and taurine, uses surfactants to increase the spreading area, and jointly greatly improves the cadmium reduction effect of brown rice. Compared with the blank group and comparative example group 7, the cadmium reduction efficiency can reach more than 85%. The technical problem that the foliar barrier control agent in the prior art can only reduce the cadmium in brown rice to about 60% at most is solved.

[0074] In summary, the present invention discloses a composite foliar barrier and application thereof in reducing cadmium accumulation in brown rice. In the composite foliar barrier, NaHS concentration is 10-20 μmol / L, melatonin concentration is 20-30 μmol / L, taurine concentration is 0.1-0.5 g / L, surfactant concentration is 0.1-0.3 g / L, and the rest is water. The composite foliar barrier is sprayed in the tillering stage, heading stage and filling stage of rice, respectively, and the spraying amount per mu is 30-50 kg each time. The present invention utilizes the synergistic effect of NaHS, melatonin and taurine, adopts surfactant to increase the spreading area, and jointly greatly improves the cadmium reduction effect of brown rice, and the cadmium reduction efficiency can reach more than 85%. The foliar barrier of the present invention has simple ingredients, is easy to operate, and has outstanding effects.

[0075] At this point, those skilled in the art recognize that, although the embodiments of the present invention have been shown and described in detail herein, many other variations or modifications that conform to the principles of the present invention can still be directly determined or derived based on the contents disclosed in the present invention without departing from the spirit and scope of the present invention. Therefore, the scope of the present invention should be understood and recognized as covering all such other variations or modifications.

Claims

1. A composite leaf surface control agent, characterized in that: The composition formula of the composite leaf surface barrier agent is: The concentration of NaHS is 10-20 μmol / L; the concentration of melatonin is 20-30 μmol / L; the concentration of taurine is 0.1-0.5 g / L; the concentration of surfactant is 0.1-0.3 g / L; the balance is water; In the composite leaf surface barrier agent, the surfactant is Tween 80 or OP-10.

2. A composite leaf surface control agent as claimed in claim 1, characterized in that: The composition formula of the composite leaf surface barrier agent is: NaHS: 20 μmol / L; melatonin: 30 μmol / L; taurine: 0.3 g / L; surfactant concentration is 0.2 g / L; the balance is water.

3. A method for preparing a composite leaf surface barrier agent as claimed in claim 1, characterized in that: The preparation method is: Weigh NaHS, melatonin, taurine, surfactant and water according to the concentration requirements and preparation amount; directly put NaHS into water to dissolve, stir evenly, then add melatonin, taurine and surfactant in sequence, stir and dissolve evenly to obtain the composite foliar barrier agent, which should be prepared before use and should not be stored for more than 6 hours.

4. The method for applying the composite foliar barrier agent as claimed in claim 1, characterized in that: The application method is: The composite foliar barrier agent is sprayed at the tillering stage, heading stage and grain filling stage of rice, with a spraying amount of 30-50 kg per mu each time.

5. The method for applying the composite foliar barrier agent as claimed in claim 4, characterized in that: The spraying parts are the leaf surface and the back of the rice leaves.

6. A method for applying a composite foliar barrier agent as claimed in claim 4, characterized in that: The composite foliar barrier agent is sprayed before 10 am and after 4 pm on sunny or cloudy days.

7. A method for applying a composite foliar barrier agent as claimed in claim 4, characterized in that: The suitable temperature range for spraying the composite foliar barrier agent is 15-30°C.

8. The composite leaf surface barrier agent according to any one of claims 1 to 2, characterized in that: The composite foliar barrier agent is used to reduce cadmium accumulation in brown rice.

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