Gel material for targeted ecological prevention and treatment of caries of root and surface as well as preparation method and application of gel material

Abstract

The invention relates to the technical field of medical materials, in particular to a gel material for targeted ecological prevention and treatment of caries of a root surface as well as a preparation method and application thereof. The root face caries gel material comprises sodium houttuyfonate and methyl cellulose, and the mass m1 of the sodium houttuyfonate and the mass m2 of the methyl cellulose meet the relational expression: m1: m2 = (0.5-2): (10-20). According to the root-face caries gel material, through the synergistic effect between sodium houttuyfonate and methyl cellulose, the antibacterial ability and adhesive ability of the gel material can be effectively improved.

Description

Technical Field

[0001] The present application relates to the technical field of medical materials, and in particular to a targeted ecological root caries prevention and treatment gel material and a preparation method and application thereof. Background Art

[0002] Root caries is a common oral disease that generally occurs at the root of the tooth, which makes the treatment and prevention of root caries relatively difficult. At present, root caries is the main cause of oral pain and tooth loss in the elderly. Traditional prevention and treatment methods for root caries include the use of fluoride, drug therapy and filling therapy, but these methods have certain limitations. For example, fluoride has limited therapeutic effect on root caries that have already formed substantial defects, while drug therapy and filling therapy may cause further damage to the teeth. Therefore, the development of new gel materials for the prevention and treatment of root caries is of great clinical significance.

[0003] Although gel materials can be used to fill the defective parts of root caries at this stage, it is difficult for gel materials to effectively inhibit the growth of caries-causing bacteria in the oral cavity, causing the gel materials to be easily decomposed by caries-causing bacteria, which not only increases the number of caries-causing bacteria, but also affects the treatment effect of root caries; in addition, gel materials are difficult to effectively adhere to the surface of root caries, and it is difficult to promote the re-mineralization of the root caries part, making the treatment effect of root caries unsatisfactory. Summary of the invention

[0004] The present application provides a targeted ecological root caries prevention and treatment gel material and a preparation method and application thereof, in order to solve the following technical problem: how to improve the antibacterial ability and adhesion ability of the gel material.

[0005] In a first aspect, an embodiment of the present application provides a targeted ecological root caries prevention and treatment gel material, wherein the root caries gel material comprises sodium houttuynia cordata and methyl cellulose, and the mass m1 of the sodium houttuynia cordata and the mass m2 of the methyl cellulose satisfy the relationship: m1:m2=(0.5~2):(10~20).

[0006] Optionally, the mass m1 of the sodium houttuynia cordata and the mass m3 of the root caries gel material satisfy the relationship: m1:m3=(0.5-2):100.

[0007] Optionally, the mass m2 of the methyl cellulose and the mass m3 of the root caries gel material satisfy the relationship: m2:m3=(10-20):100.

[0008] Optionally, the viscosity of the root caries gel material is 7×10 5 mPa·s~400×10 5 mPa·s.

[0009] Optionally, the Actinomyces inhibition rate of the root surface caries gel material is ≥99%, and the Candida albicans inhibition rate of the root surface caries gel material is ≥93%.

[0010] In a second aspect, the present application provides a method for preparing the root caries gel material according to the first aspect, the method comprising:

[0011] Mixing houttuynia cordata sodium and a solvent under preset conditions to obtain a houttuynia cordata sodium solution;

[0012] The methylcellulose and the sodium houttuyfonate solution are subjected to gel reaction to obtain a root caries gel material.

[0013] Optionally, the preset conditions include a preset temperature and a preset time, the preset temperature is 50° C. to 80° C., and the preset time is 30 min to 60 min.

[0014] Optionally, the temperature of the gel reaction is 35° C. to 45° C., and the time of the gel reaction is 20 min to 60 min.

[0015] Optionally, the step of mixing sodium houttuyfonate and a solvent under preset conditions to obtain a sodium houttuyfonate solution comprises the following steps:

[0016] dissolving houttuynia cordata sodium in a solvent to obtain a mixture;

[0017] The mixture is stirred and mixed under preset conditions, and then cooled to obtain a sodium houttuynia cordata solution;

[0018] Wherein, the stirring and mixing speed is ≥100r / min, and the stirring and mixing time is ≥30min.

[0019] In a third aspect, the present application provides an application of the root caries gel material described in the first aspect in the preparation of medicines, daily necessities and medical devices related to the treatment and prevention of root caries.

[0020] The above technical solution provided by the embodiment of the present application has the following advantages compared with the prior art:

[0021] The embodiment of the present application provides a targeted ecological root caries prevention and treatment gel material, which may include sodium houttuynia cordata. Based on the antibacterial and anti-inflammatory effects of sodium houttuynia cordata, the root caries gel material has good antibacterial and anti-inflammatory effects and can effectively inhibit the growth of caries-causing bacteria. In addition, the root caries gel material may include methylcellulose. Based on the good gelling properties, biocompatibility and degradability of methylcellulose, the root caries gel material can be effectively mixed with sodium houttuynia cordata and adhered to the root caries site of the tooth, thereby effectively improving the root caries prevention and treatment effect. The adhesion of the root caries gel material is high, so as to effectively and slowly release sodium houttuynia cordata; in addition, the root caries gel material also has a thermally reversible gelling property, so that the root caries gel material can still maintain a gel state under the temperature conditions of the human oral cavity, and can further enhance the antibacterial and anti-inflammatory effects of sodium houttuynia cordata to maintain the normal function of the tooth hard tissue, thereby promoting the remineralization of the tooth hard tissue to repair the tooth part with root caries; therefore, the root caries gel material can effectively enhance the antibacterial ability and adhesion ability of the gel material through the synergistic effect between sodium houttuynia cordata and methylcellulose. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present application and, together with the description, serve to explain the principles of the present application.

[0023] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0024] Figure 1 A schematic flow chart of a method for preparing the root caries gel material provided in an embodiment of the present application;

[0025] Figure 2 A detailed schematic diagram of a method for preparing the root caries gel material provided in an embodiment of the present application;

[0026] Figure 3 This is a comparative result diagram of the injectability characterization of the root caries gel materials of Example 2, Example 1, Example 3 and Comparative Example 1 provided in the present application;

[0027] Figure 4 A graph showing the viscosity comparison results of the root caries gel materials of Example 2, Example 1, Example 3 and Comparative Example 1 provided in the present application;

[0028] Figure 5A graph comparing the strain-modulus curves of the root caries gel materials of Example 2, Example 1, Example 3 and Comparative Example 1 provided in the present application;

[0029] Figure 6 A graph comparing the stress-modulus curves of the root caries gel materials of Example 2, Example 1, Example 3 and Comparative Example 1 provided in the present application;

[0030] Figure 7 This is a comparison chart of the antibacterial test results of the root caries gel materials of Example 2, Example 1, Example 3 and Comparative Example 1 provided in the present application; wherein, Figure 7 a is a comparison result of the anti-adhesive actinomycetes of the root caries gel materials of Example 2, Example 1, Example 3 and Comparative Example 1, Figure 7 b is a comparison of the results of Candida albicans in the root caries gel materials of Example 2, Example 1, Example 3 and Comparative Example 1

[0031] Figure 8 This is a comparison chart of drug release experiments on the root caries gel materials of Example 2, Example 1, and Example 3 provided in the present application. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application.

[0033] Various embodiments of the present application may be presented in the form of a range; it should be understood that the description in the form of a range is only for convenience and brevity and should not be understood as a rigid limitation on the scope of the present application; therefore, it should be considered that the range description has specifically disclosed all possible sub-ranges and single numerical values ​​within the range; for example, it should be considered that the range description from 1 to 6 has specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., as well as single numbers within the range, such as 1, 2, 3, 4, 5 and 6, which applies regardless of the range; in addition, whenever a numerical range is indicated in this document, it is meant to include any cited number (fractional or integer) within the indicated range.

[0034] In this document, the terms including "including" and "including" mean "including but not limited to". Relational terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. "And / or" describes the association relationship of associated objects, indicating that there can be three relationships. For example, A and / or B can mean: A exists alone, A and B exist at the same time, and B exists alone; where A and B can be singular or plural. "At least one" means one or more, and "plurality" means two or more; "at least one", "at least one of the following" or similar expressions, refers to any combination of these items, including any combination of single or plural items; for example, "at least one of a, b, or c", or "at least one of a, b, and c" can all mean: a, b, c, ab (i.e., a and b), ac, bc, or abc, where a, b, and c can be single or multiple. "Parts" such as parts by weight and parts by mass indicate the proportional relationship between the components. In the proportional relationship involved in this article, the parameters that need to be described by proportion should be understood as the first term of the proportional formula in the order of description, and the proportional numbers should be understood as the second term of the proportional formula. For example, if the mass ratio of substance A, substance B and substance C is 1:2:3, then substance A, substance B and substance C should correspond to the proportional numbers in the proportional formula in the order of description, that is, the mass of substance A: the mass of substance B: the mass of substance C = 1:2:3.

[0035] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in this article can be purchased from the market or prepared by existing methods.

[0036] An embodiment of the present application provides a targeted ecological root caries prevention and treatment gel material, wherein the root caries gel material comprises sodium houttuynia cordata and methyl cellulose, wherein the mass m1 of the sodium houttuynia cordata and the mass m2 of the methyl cellulose satisfy the relationship: m1:m2=(0.5-2):(10-20).

[0037] The mass m1 of the sodium houttuynia cordata can be 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9 or 2.0.

[0038] The mass m2 of the methyl cellulose can be 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20.

[0039] It should be noted that sodium houttuynia cordata is a natural compound extracted from Houttuynia cordata, which has the characteristics of antibacterial and anti-inflammatory effects, immunity enhancement, antiviral activity, cardiovascular protection and safety. It can play an antibacterial role in the treatment of root caries while promoting the remineralization of tooth hard tissue, thereby effectively restoring the hardness and structure of teeth.

[0040] It should be noted that methylcellulose has good biocompatibility, degradability, thermoreversible gelation properties and solution stability, and is a hydrogel base material. Methylcellulose has the characteristics of low cost, simple preparation process and gelation conditions. In addition, the preparation steps of methylcellulose are simple. Methylcellulose is used as an anti-root caries gel material. Based on its degradability, the root caries gel material can avoid secondary surgery and reduce inflammatory response. In addition, the thermoreversible gelation property of the root caries gel material allows the hydrogel to maintain a gel state at the temperature of the human oral cavity, which helps the sustained release of sodium houttuynia cordata, thereby improving the targeted treatment effect of the root caries gel material.

[0041] It should be noted that the embodiment of the present application provides a targeted ecological root caries prevention gel material, which not only innovatively combines sodium houttuynia cordata and methylcellulose, but also achieves efficient prevention and treatment of root caries through its unique material design. The specific analysis is as follows:

[0042] (1) One of the core components of the root caries gel material is houttuynia cordata sodium, which is selected for its significant antibacterial and anti-inflammatory effects. Houttuynia cordata sodium can effectively inhibit the growth of caries-causing bacteria and reduce harmful bacteria in the oral cavity, thereby providing a healthier ecological environment for teeth. This property makes the root caries gel material have significant advantages in preventing and treating root caries.

[0043] (2) In addition to sodium houttuynia cordata, methylcellulose is also added to the material. Methylcellulose, with its good gelling properties, biocompatibility and degradability, has become another important component of the root caries gel material. It can be effectively mixed with sodium houttuynia cordata to form a stable gel system. This gel can not only adhere tightly to the root caries area of ​​the tooth, but also slowly release sodium houttuynia cordata to ensure that the drug ingredients can continue to work stably. This design not only improves the adhesion of the root caries gel material, but also prolongs its antibacterial and anti-inflammatory effects.

[0044] (3) What is even more unique is that the root caries gel material also has a thermoreversible gelation property. This means that under the temperature conditions of the human oral cavity, the material can still maintain a gel state and will not lose its original form and function due to temperature changes. This property further enhances the antibacterial and anti-inflammatory effects of houttuynia cordata sodium, allowing the material to play a more stable and effective therapeutic role in the oral environment.

[0045] In summary, the embodiment of the present application provides a targeted ecological root caries prevention and treatment gel material, which achieves efficient prevention and treatment of root caries through the synergistic effect between sodium houttuynia cordata and methylcellulose, as well as the unique thermally reversible gelation characteristics. It not only has good antibacterial and anti-inflammatory effects, but can also adhere tightly to the tooth surface, continuously and stably release drug ingredients, and provide a long-term protective barrier for the teeth. This innovative material design provides new ideas and methods for the prevention and treatment of root caries.

[0046] In some optional embodiments, the mass m1 of the sodium houttuynia cordata and the mass m3 of the root caries gel material satisfy the relationship: m1:m3=(0.5-2):100;

[0047] In these embodiments, the mass m1 of sodium houttuynia cordata and the mass m3 of the root caries gel material can satisfy the relationship: m1:m3=(0.5-2):100, which ensures that the root caries gel material has a sufficient amount of sodium houttuynia cordata. A sufficient amount of sodium houttuynia cordata has good antibacterial and anti-inflammatory effects and can effectively inhibit the growth of caries-causing bacteria.

[0048] The mass m1 of the sodium houttuynia cordata can be 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9 or 2.0.

[0049] In some optional embodiments, the mass m2 of the methyl cellulose and the mass m3 of the root caries gel material satisfy the relationship: m2:m3=(10-20):100;

[0050] In these embodiments, the mass m2 of methylcellulose and the mass m3 of the root caries gel material can satisfy the relationship: m2:m3=(10-20):100, which ensures that the root caries gel material has a sufficient amount of methylcellulose. On the one hand, a sufficient amount of methylcellulose can effectively improve the adhesion of the root caries gel material to effectively and slowly release sodium houttuynia cordata, and on the other hand, it can maintain the normal function of the tooth hard tissue, thereby promoting the remineralization of the tooth hard tissue to repair the tooth site with root caries.

[0051] The mass m2 of the methyl cellulose can be 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or 20.

[0052] In some optional embodiments, the viscosity of the root caries gel material is 7×10 5 mPa·s~400×10 5 mPa·s;

[0053] In some optional embodiments, the inhibition rate of Actinomyces viscosus of the root caries gel material is ≥99%, and the inhibition rate of Candida albicans of the root caries gel material is ≥93%;

[0054] In these embodiments, the viscosity stress of the root caries gel material may be 7×10 5 mPa·s~400×10 5 mPa·s, and the inhibition rate of Actinomycetes of root surface caries gel material is ≥99%, and the inhibition rate of Candida albicans of root surface caries gel material is ≥93%, which makes the root surface caries gel material have better adhesion and antibacterial ability.

[0055] The shear stress of the root caries gel material can be 7×10 5 mPa·s, 10×10 5 mPa·s, 20×10 5 mPa·s, 30×10 5 mPa·s, 40×10 5 mPa·s, 50×10 5 mPa·s, 60×10 5 mPa·s, 70×10 5 mPa·s, 80×10 5 mPa·s, 90×10 5 mPa·s, 100×10 5 mPa·s, 150×10 5 mPa·s, 200×10 5 mPa·s, 250×10 5 mPa·s、300×10 5 mPa·s, 350×10 5 mPa·s or 400×10 5 mPa·s.

[0056] Figure 1 A schematic flow chart of a method for preparing the root caries gel material provided in an embodiment of the present application is exemplarily shown;

[0057] Based on a general inventive concept, such as Figure 1 As shown, the embodiment of the present application provides a method for preparing the root caries gel material, the method comprising:

[0058] S1. Mixing houttuynia cordata sodium and a solvent under preset conditions to obtain a houttuynia cordata sodium solution;

[0059] S2. subjecting methylcellulose and the sodium houttuyfonate solution to a gel reaction to obtain a root caries gel material.

[0060] This method is a method for preparing the above-mentioned root caries gel material. The specific composition of the root caries gel material can refer to the above-mentioned embodiment. Since this method adopts part or all of the technical solutions of the above-mentioned embodiment, it at least has all the beneficial effects brought by the technical solutions of the above-mentioned embodiment, which will not be repeated here one by one.

[0061] It should be noted that the solvent can be deionized water.

[0062] In some optional embodiments, the preset conditions include a preset temperature and a preset time, the preset temperature is 50° C. to 80° C., and the preset time is 30 min to 60 min;

[0063] In these embodiments, the preset conditions may include a preset temperature and a preset time, and the preset temperature may be 50°C to 80°C, and the preset time may be 30min to 60min, so that the sodium houttuynia cordata is fully dissolved by the solvent, thereby obtaining a uniformly dispersed sodium houttuynia cordata solution.

[0064] The preset temperature may be 50°C, 55°C, 60°C, 65°C, 70°C, 75°C or 80°C.

[0065] The preset time may be 30 min, 35 min, 40 min, 45 min, 50 min, 55 min or 60 min.

[0066] In some optional embodiments, the temperature of the gel reaction is 35°C to 45°C, and the time of the gel reaction is 20min to 60min;

[0067] In these embodiments, the temperature of the gel reaction can be 35°C to 45°C, and the time of the gel reaction can be 20min to 60min, so that the methylcellulose is fully dissolved in the sodium houttuynia cordata solution and forms a gel material. The formed gel material can, on the one hand, effectively improve the adhesion of the root caries gel material to effectively and slowly release sodium houttuynia cordata, and on the other hand, maintain the normal function of the tooth hard tissue, thereby promoting the remineralization of the tooth hard tissue to repair the tooth site with root caries.

[0068] The temperature of the gel reaction can be 35°C, 36°C, 37°C, 38°C, 39°C, 40°C, 41°C, 42°C, 43°C, 44°C or 45°C.

[0069] The gel reaction time can be 20 min, 25 min, 30 min, 35 min, 40 min, 45 min, 50 min, 55 min or 60 min.

[0070] Figure 2A detailed schematic diagram of a method for preparing the root caries gel material provided in an embodiment of the present application is exemplarily shown;

[0071] In some optional embodiments, such as Figure 2 As shown, the process of mixing sodium houttuynia cordata and a solvent under preset conditions to obtain a sodium houttuynia cordata solution comprises the following steps:

[0072] S101. Dissolving houttuynia cordata sodium in a solvent to obtain a mixture;

[0073] S102. The mixture is stirred and mixed under preset conditions, and then cooled to obtain a sodium houttuynia cordata solution;

[0074] In these embodiments, the sodium houttuyfonate and the solvent are stirred and mixed, so that the sodium houttuyfonate can be fully dissolved in the solvent, thereby obtaining a sodium houttuyfonate solution in which the sodium houttuyfonate is evenly dispersed.

[0075] It should be noted that the end point temperature of the cooling is room temperature.

[0076] In some optional embodiments, the stirring and mixing speed is ≥100 r / min, and the stirring and mixing time is ≥30 min;

[0077] In these embodiments, the stirring and mixing speed is ≥100 r / min, and the stirring and mixing time is ≥30 min, so that the sodium houttuyfonate and the solvent are fully mixed to obtain a sodium houttuyfonate solution in which the sodium houttuyfonate is evenly dispersed.

[0078] The rotation speed of the stirring and mixing can be 100r / min, 110r / min, 120r / min, 130r / min, 140r / min or 150r / min.

[0079] The stirring and mixing time can be 30 min, 35 min, 40 min, 45 min, 50 min, 55 min or 60 min.

[0080] Based on a general inventive concept, an embodiment of the present application provides an application of the root caries gel material in the preparation of medicines, daily necessities and medical equipment supplies related to the treatment and prevention of root caries.

[0081] This application is realized based on the above-mentioned root caries gel material. The specific composition of the root caries gel material can refer to the above-mentioned embodiment. Since this application adopts part or all of the technical solutions of the above-mentioned embodiments, it has at least all the beneficial effects brought by the technical solutions of the above-mentioned embodiments, which will not be repeated here one by one.

[0082] The present application is further described below in conjunction with specific examples. The experimental methods in the following examples where specific conditions are not specified are usually measured in accordance with national standards / industry standards; if there are no corresponding national standards / industry standards, they are measured in accordance with common international standards, conventional conditions or conditions recommended by the manufacturer.

[0083] Example 1

[0084] A targeted ecological root caries prevention and treatment gel material comprises sodium houttuynia cordata and methyl cellulose. The mass m1 of the sodium houttuynia cordata and the mass m2 of the methyl cellulose satisfy the relationship: m1:m2=0.5:12.

[0085] The mass m1 of sodium houttuynia cordata and the mass m3 of the root caries gel material satisfy the relationship: m1:m3=0.5:100.

[0086] The mass m2 of methylcellulose and the mass m3 of root caries gel material satisfy the relationship: m2:m3=12:100.

[0087] The viscosity of root caries gel material is 7×105~90×105.

[0088] like Figure 2 As shown, a method for preparing a root caries gel material comprises:

[0089] S101. Dissolve 0.005 g of sodium houttuynia cordata in 1 mL of deionized water to obtain a mixture;

[0090] S102. The mixture is stirred and mixed under preset conditions, and then cooled to obtain a sodium houttuynia cordata solution;

[0091] S2. Stir 0.12g of methylcellulose and sodium houttuyfonate solution at a speed of 100 r / min for 30 min to completely dissolve the methylcellulose and simultaneously perform a gel reaction to obtain a root caries gel material.

[0092] The preset conditions include a preset temperature and a preset time. The preset temperature is 60°C and the preset time is 30 minutes.

[0093] The temperature of the gel reaction was 37°C, and the time of the gel reaction was 30 min.

[0094] The stirring speed is 100 r / min, and the stirring time is 30 min.

[0095] Example 2

[0096] Based on the contents disclosed in Example 1, the following modifications are further made:

[0097] The mass m1 of sodium houttuynia cordata and the mass m2 of methylcellulose satisfy the relationship: m1:m2=2:10. The mass m1 of sodium houttuynia cordata is 0.02g, and the mass m2 of methylcellulose is 0.10g.

[0098] The mass m1 of sodium houttuynia cordata and the mass m3 of the root caries gel material satisfy the relationship: m1:m3=2:100.

[0099] The mass m2 of methylcellulose and the mass m3 of root caries gel material satisfy the relationship: m2:m3=10:100.

[0100] Example 3

[0101] Based on the contents disclosed in Example 1, the following modifications are further made:

[0102] The mass m1 of sodium houttuynia cordata and the mass m2 of methylcellulose satisfy the relationship: m1:m2=1:20. The mass m1 of sodium houttuynia cordata is 0.01 g, and the mass m2 of methylcellulose is 0.20 g.

[0103] The mass m1 of sodium houttuynia cordata and the mass m3 of the root caries gel material satisfy the relationship: m1:m3=1:100.

[0104] The mass m2 of methylcellulose and the mass m3 of root caries gel material satisfy the relationship: m2:m3=20:100.

[0105] Example 4

[0106] Based on the contents disclosed in Example 2, the following modifications are further made:

[0107] The preset temperature is 50℃ and the preset time is 60min.

[0108] The temperature of the gel reaction was 35°C, and the time of the gel reaction was 60 min.

[0109] Example 5

[0110] Based on the contents disclosed in Example 2, the following modifications are further made:

[0111] The preset temperature is 80℃ and the preset time is 40min.

[0112] The temperature of the gel reaction was 45°C, and the time of the gel reaction was 20 min.

[0113] Comparative Example 1

[0114] Based on the contents disclosed in Example 2, the following modifications are further made:

[0115] No houttuynia cordata sodium was added.

[0116] Comparative Example 2

[0117] Based on the contents disclosed in Example 2, the following modifications are further made:

[0118] Methyl cellulose was replaced by sodium hydroxymethyl cellulose, and the temperature of the gel reaction was adjusted to 44°C, and the gel reaction time was the same.

[0119] Comparative Example 3

[0120] Based on the contents disclosed in Example 2, the following modifications are further made:

[0121] The mass m1 of sodium houttuynia cordata and the mass m2 of methylcellulose satisfy the relationship: m1:m2=4:12.

[0122] The mass m1 of sodium houttuynia cordata and the mass m3 of the root caries gel material satisfy the relationship: m1:m3=4:100.

[0123] The mass m2 of methylcellulose and the mass m3 of root caries gel material satisfy the relationship: m2:m3=12:100.

[0124] Comparative Example 4

[0125] Based on the contents disclosed in Example 2, the following modifications are further made:

[0126] The preset temperature is 30℃ and the preset time is 70min.

[0127] Comparative Example 5

[0128] Based on the contents disclosed in Example 2, the following modifications are further made:

[0129] The preset temperature is 100°C and the preset time is 20 minutes.

[0130] Comparative Example 6

[0131] Based on the contents disclosed in Example 2, the following modifications are further made:

[0132] The temperature of the gel reaction was 30°C, and the time of the gel reaction was 70 min.

[0133] Comparative Example 7

[0134] Based on the contents disclosed in Example 2, the following modifications are further made:

[0135] The temperature of the gel reaction was 60°C, and the time of the gel reaction was 10 min.

[0136] Related experiments and effect data:

[0137] 1. Collect the root caries gel materials obtained in Examples 1 to 3 and Comparative Example 1 respectively, and conduct the following experiments:

[0138] (1) Characterization of injectability: A 1 mL syringe and needles of four sizes, 26G, 18G, 17G, and 15G, were used to extrude root caries gel materials with a mass concentration of 0%, 0.5%, 1.0%, and 2.0% of houttuynia cordata sodium, respectively, at a certain speed.

[0139] (2) Rheological property test: The viscosity, strain-modulus curve and stress-modulus curve of the root caries gel material with different drug loading concentrations (mass concentration of 0%, 0.5%, 1.0%, and 2.0%) were tested using a rheometer.

[0140] (3) Antibacterial experiment: Root caries gel materials with different drug loading concentrations (mass concentration of 0%, 0.5%, 1.0%, and 2.0%) were immersed in a solution containing Actinomyces viscosus (1×10 6 CFU / mL and Candida albicans (1×10 4 CFU / mL) of bacterial suspension, the volume ratio of immersion is 1:1. Co-culture at 37℃ for 24h, then use physiological saline to dilute each bacterial suspension in a ratio of 1:10 by volume, and then evenly spread the diluted bacterial suspension on the BHY agar plate. The specimen is anaerobically cultured at 37℃ for 24h. Then select a suitable agar plate (30 to 300 colony capacity) and calculate the number of viable bacteria (CFU / mL) in the original resuspension.

[0141] (4) Drug release experiment: 200 μL of aqueous solution of sodium houttuynia cordata with mass concentration of 0.5%, 1.0%, and 2.0% was absorbed into the mold, and then incubated in a water bath to form a gel. The total mass of the hydrogel and the mass of the drug were weighed, and the content of sodium houttuynia cordata drug in the mold was calculated in proportion. The drug release experiment was carried out by placing 3 mL of Ro water, and 350 μL of the release solution was measured each time, and 350 μL of Ro water was added after taking it. The cumulative sampling time points were 1h, 2h, 4h, 6h, 8h, 12h, and 24h. The absorbance of the release solution was measured by an ELISA instrument, and a standard curve was established. The concentration of the release solution was calculated by the linear relationship between concentration and absorbance.

[0142] The results of the above experiments are as follows Figures 3 to 7 As shown, Figure 3The results show that Example 2 using 1% sodium houttuynia cordata has good injectability compared with Example 1 (0.5% sodium houttuynia cordata), Example 3 (2.0% sodium houttuynia cordata) and Comparative Example 1 (0.0% sodium houttuynia cordata), which allows the root caries gel material to be accurately placed and retained in the defective area of ​​the tooth, greatly reducing the possibility of off-target effects and ensuring that the treatment is concentrated on a specific defective area.

[0143] Figure 4 The results show that: Example 2 using 1% sodium houttuynia cordata has higher shear performance and better viscosity than Example 1 (0.5% sodium houttuynia cordata), Example 3 (2.0% sodium houttuynia cordata) and Comparative Example 1 (0.0% sodium houttuynia cordata), and the hydrogel of the highly viscous root caries gel material can be used as a drug carrier to achieve the sustained release of sodium houttuynia cordata, which can reduce the inflammation of root caries and attract host cells to the defective parts of the teeth, thereby achieving the treatment of root caries. In addition, the highly viscous root caries gel material can more effectively adhere to the surface of teeth and oral tissues, prolong the retention time of sodium houttuynia cordata in the lesion site, thereby further improving the therapeutic effect of root caries.

[0144] Figure 5 and Figure 6 The results show that in the strain-modulus curve and stress-modulus curve tests, Example 2 using 1% sodium houttuynia cordata has better gelling effect under a wide range of shear stress than Example 1 (0.5% sodium houttuynia cordata), Example 3 (2.0% sodium houttuynia cordata) and Comparative Example 1 (0.0% sodium houttuynia cordata). The root caries gel material with high gelling property can firmly adhere to the tooth surface, thereby promoting the remineralization of tooth hard tissue. At the same time, the sodium houttuynia cordata in the root caries gel material, as a bioactive molecule, can inhibit the biofilm of caries-causing bacteria based on its antioxidant and anti-inflammatory properties, and promote the remineralization of tooth hard tissue in the early stage of caries. Therefore, the root caries gel material with high gelling property has antibacterial effect and certain anti-caries potential.

[0145] Figure 7 The results show that Example 2 using 1% sodium houttuynia cordata showed a significant inhibitory effect on the growth of Actinomycetes and Candida albicans compared with Example 1 (0.5% sodium houttuynia cordata), Example 3 (2.0% sodium houttuynia cordata) and Comparative Example 1 (0.0% sodium houttuynia cordata), which indicates that the root caries gel material has good anti-Actinomycetes and anti-Candida albicans effects.

[0146] Figure 8 The results show that Example 2 using 1% sodium houttuynia cordata and Example 3 (2.0% sodium houttuynia cordata) have a faster drug release rate than Example 1 (0.5% sodium houttuynia cordata). Therefore, considering the preparation cost and drug effect, the 1% sodium houttuynia cordata used in Example 2 has both a more suitable time to reach the target drug release concentration and a good antibacterial effect.

[0147] 2. The root caries gel materials of each embodiment and comparative example were collected respectively, and the viscosity and antibacterial rate of the rheological properties were statistically compared with comparative example 1. The results are shown in Table 1.

[0148] Table 1 Rheological properties and antibacterial rate of root caries gel materials of various embodiments and comparative examples

[0149]

[0150]

[0151] It can be seen from Table 1 that each embodiment has a higher critical stress of rheological properties and a higher antibacterial rate (99% and above), which indicates that the root caries gel material of each embodiment has a higher adhesion and antibacterial rate.

[0152] In addition, although comparative example 1 has good adhesion, its antibacterial effect is not strong. This may be because the gel formed by methylcellulose itself has a physical isolation effect on teeth and blocks the invasion of caries bacteria; although comparative example 2 uses sodium hydroxymethyl cellulose as the gel matrix material, the gel formed by it also has high adhesion, but because the critical temperature of the gel reaction of sodium hydroxymethyl cellulose is 40°C to 45°C, it is difficult to decompose sodium hydroxymethyl cellulose under the temperature conditions of the human oral cavity, so that sodium houttuynia cordata is released, resulting in a low final antibacterial rate; in addition, in comparative example 3, excessive sodium houttuynia cordata is used, which will lead to uneven distribution of the root caries gel material. Although it has a high antibacterial rate, it will affect the dispersion of the overall gel material and its adhesion; in addition, comparative examples 4 and 5 respectively use Using a lower preset temperature and a higher preset temperature, the lower preset temperature will lead to insufficient dissolution of sodium houttuynia cordata, reduce the antibacterial components of the root caries gel material and uneven distribution of the gel material, affecting both its adhesion and its antibacterial rate, while the higher preset temperature will affect the activity of sodium houttuynia cordata, causing the sodium houttuynia cordata to aggregate into lumps, further affecting the antibacterial components of the root caries gel material and the degree of dispersion of the gel material, affecting both its adhesion and its antibacterial rate; in addition, Example 6 uses a lower gel reaction temperature, which makes the root caries gel material difficult to form, ultimately affecting the adhesion and antibacterial rate of the root caries gel material; in addition, Example 7 uses a higher gel reaction temperature, which will lead to the inactivation of sodium houttuynia cordata and the aggregation of methylcellulose, affecting both its adhesion and its antibacterial rate.

[0153] In summary, the embodiment of the present application provides a targeted ecological root caries prevention and treatment gel material, which achieves efficient prevention and treatment of root caries through the synergistic effect between sodium houttuynia cordata and methylcellulose, as well as the unique thermally reversible gelation characteristics. It not only has good antibacterial and anti-inflammatory effects, but can also adhere tightly to the tooth surface, continuously and stably release drug ingredients, and provide a long-term protective barrier for the teeth. This innovative material design provides new ideas and methods for the prevention and treatment of root caries.

[0154] In addition, the embodiment of the present application provides a targeted ecological root caries prevention and treatment gel material, which can effectively prevent and treat root caries without damaging tooth tissue; in addition, the root caries gel material can also promote the remineralization of tooth hard tissue by regulating the ecological balance of oral microorganisms and inhibiting the growth of cariogenic bacteria, thereby achieving targeted treatment of root caries.

[0155] In addition, the embodiment of the present application provides an application of a targeted ecological root caries prevention and treatment gel material. Based on its good targeted treatment effect, the root caries gel material can be used to prepare medicines related to the treatment and prevention of root caries, and can also be used to prepare daily necessities related to the treatment and prevention of root caries, as well as to prepare medical equipment and supplies related to the treatment and prevention of root caries.

[0156] The above description is only a specific implementation of the present application, so that those skilled in the art can understand or implement the present application. It will be apparent to those skilled in the art that various modifications to these embodiments are possible, and the general principles defined in the present application can be implemented in other embodiments without departing from the spirit or scope of the present application. Therefore, the present application will not be limited to the embodiments shown in the present application, but will conform to the widest range consistent with the principles and novel features applied for by the present application.

Claims

1. A targeted ecological root caries prevention and treatment gel material, the root caries gel material comprising sodium houttuynia cordata and methyl cellulose, the mass m1 of the sodium houttuynia cordata and the mass m2 of the methyl cellulose satisfying the relationship: m1:m2=(0.5-2):(10-20).

2. The root caries gel material according to claim 1, wherein the mass m1 of the sodium houttuyfonate and the mass m3 of the root caries gel material satisfy the relationship: m1:m3=(0.5-2):

100.

3. The root surface caries gel material according to claim 1, wherein the mass m2 of the methyl cellulose and the mass m3 of the root surface caries gel material satisfy the relationship: m2:m3=(10-20):

100.

4. The root surface caries gel material according to claim 1, wherein the viscosity of the root surface caries gel material is 7×10 5 mPa·s~400×10 5 mPa·s. 5 . The root caries gel material according to claim 1 , wherein the Actinomycetes inhibition rate of the root caries gel material is ≥ 99%, and the Candida albicans inhibition rate of the root caries gel material is ≥ 93%.

6. A method for preparing the root caries gel material according to any one of claims 1 to 5, the method comprising: Mixing houttuynia cordata sodium and a solvent under preset conditions to obtain a houttuynia cordata sodium solution; The methylcellulose and the sodium houttuyfonate solution are subjected to gel reaction to obtain a root caries gel material.

7. The method according to claim 6, wherein the preset conditions include a preset temperature and a preset time, the preset temperature is 50°C to 80°C, and the preset time is 30min to 60min.

8. The method according to claim 6, wherein the temperature of the gel reaction is 35°C to 45°C, and the time of the gel reaction is 20 min to 60 min.

9. The method according to claim 6, wherein the sodium houttuyfonate and the solvent are mixed under preset conditions to obtain the sodium houttuyfonate solution, comprising the steps of: dissolving houttuynia cordata sodium in a solvent to obtain a mixture; The mixture is stirred and mixed under preset conditions, and then cooled to obtain a sodium houttuynia cordata solution; in, The stirring and mixing speed is ≥100r / min, and the stirring and mixing time is ≥30min.

10. Use of the root caries gel material according to any one of claims 1 to 5 in the preparation of medicines, daily necessities and medical equipment related to the treatment and prevention of root caries.

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