A method for raising seedlings of Prunus armeniaca and its application

Abstract

The present invention belongs to the field of plant regeneration technology through tissue culture technology, and the classification number is A01H; the present invention specifically relates to a method for raising seedlings of five-fingered peach and its application. The method for raising seedlings includes: cleaning and disinfecting the leaf tissue of five-fingered peach, then drying it, and setting it aside; placing the leaf tissue of five-fingered peach as an explant in a germination induction medium and soaking it at room temperature for 12 to 20 hours, and the germination induction medium is a 5-aminolevulinic acid-loaded modified nano zeolite dispersion; placing the soaked leaf tissue on a seedbed covered with nutrient soil according to the number of 1 to 2 leaf tissues per hole, and spraying water to moisten the soil at the same time; after 10 to 20 days of leaf tissue culture, spraying nutrient solution at a frequency of every 3 to 5 days / time, spraying until the soil is moistened each time, and when the seedling height is 10 to 15 cm, transplanting it to the field. The culture medium prepared by the present invention can improve the germination rate of five-fingered peach, promote the growth of seedlings, and reduce the cost of raising seedlings.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant regeneration through tissue culture technology, and belongs to the technical field of international classification number A01H; specifically, the present invention relates to a method for raising seedlings of Prunus mume-bark and application thereof. Background Art

[0002] Ficus hirta Vahl belongs to the genus Ficus of the Moraceae family. It is widely distributed in Guangdong, Guangxi, Yunnan, Guizhou and other places. Its tuberous roots are rich in polysaccharides, amino acids, flavonoids, polyphenols and other functional ingredients that are beneficial to the human body. It is mild in nature and sweet in taste. It has the effects of strengthening the spleen and lungs, replenishing qi and removing dampness, relieving cough and relieving asthma. It is an authentic medicinal material for both medicine and food in the Lingnan region. Although Ficus hirta Vahl has been planted on a large scale, the market demand has not been met.

[0003] Since the leaf tissue of the five-fingered peach is extremely small, the five-fingered peach seedling cultivation process faces problems such as low germination rate, slow seedling growth, long nursery seedling period, and high seedling cost. Therefore, it is urgent to design a technical solution that can improve the above defects.

[0004] There are also relevant disclosures in the prior art about the cultivation of five-fingered peach. For example, Chinese patent application CN202210590683.5 discloses a method for leaf tissue culture of five-fingered peach, with the main classification number: A01H4 / 00, which includes: selecting the top buds of five-fingered peach as explants, washing and disinfecting the explants, and absorbing the surface moisture; inoculating the explants into a cluster bud induction medium, and then transferring the induced cluster buds into the cluster bud induction medium, so that the cluster buds continue to proliferate and grow, and a large number of cluster buds and seedlings are obtained, which are used for subculture proliferation and rooting culture in the proliferation medium; transferring the robust seedlings to the rooting medium to induce rooting, and obtaining robust plants. For another example, Chinese patent application CN201410298080.3 discloses a tissue culture rapid propagation method of five-fingered peach, with the main classification number: A01H4 / 00, which comprises the following steps: (1) taking five-fingered peach leaves as explants for disinfection; (2) placing the disinfected explants in MS basic medium to induce clustered buds; (3) placing the clustered buds in MS propagation medium for propagation; (4) cutting the clustered buds into single buds and placing them in 1 / 2MS rooting medium for culture to obtain complete rooted seedlings; (5) taking the complete rooted seedlings for seedling hardening and transplanting them into a matrix with a mass ratio of peat soil: perlite = 1:1, growing in the matrix for one month and then transplanting them into the field. However, the culture methods involved in the above technologies are complicated and have a long cycle, which is not conducive to the rapid cultivation of hairy finger peach. Summary of the invention

[0005] The purpose of the present invention is to overcome the problems existing in the prior art and provide a method for raising seedlings of Prunus armeniaca and its application. The present invention uses leaf tissue as an explant and uses a culture medium to culture the explant to improve the germination rate. The method of the present invention can effectively improve the germination rate of Prunus armeniaca and reduce the cost of raising seedlings.

[0006] The purpose of the present invention and the solution to the technical problem are achieved by adopting the following technical solutions.

[0007] One aspect of the present invention provides a method for raising seedlings of Prunus mume, the method comprising:

[0008] S1: Wash and disinfect the leaf tissue of the five-fingered peach, then dry it for later use;

[0009] S2: placing the leaf tissue of Prunus armeniaca as an explant in a germination induction medium and soaking it at room temperature for 12 to 20 hours, wherein the germination induction medium is a dispersion of 5-aminolevulinic acid-loaded modified nano-zeolite;

[0010] S3: placing the soaked leaf tissues on a seedbed covered with nutrient soil at a quantity of 1 to 2 leaf tissues per hole for cultivation, and spraying water until the soil is moistened;

[0011] S4: After 10 to 20 days of leaf tissue culture, spray the nutrient solution every 3 to 5 days, spray until the soil is moist each time. When the seedlings are cultured to a height of 10 to 15 cm, transplant them to the field.

[0012] In a preferred embodiment of the present invention, in step S2, the method for preparing the germination-promoting induction medium comprises the following steps:

[0013] Dissolving cyclodextrin in an alkaline solution to obtain a cyclodextrin alkaline solution with a concentration of 20-40 wt%; then dispersing zeolite in the cyclodextrin alkaline solution at a mass volume ratio of 1 g: 10-20 mL and soaking for 8-12 hours, then taking it out to obtain cyclodextrin-coated zeolite;

[0014] The obtained cyclodextrin-coated zeolite was placed in a 10-20wt% acid solution at a mass volume ratio of 1g:10-30mL and stirred for 1-3h at 40-70°C, then cooled to room temperature, and then acidic amino acid and stabilizer were added and stirred for 4-8h, then allowed to stand and filtered, and the obtained product was washed, dried, and then crushed through a 800-1000 mesh sieve to obtain a modified nano zeolite;

[0015] The modified nano zeolite was ultrasonically dispersed in a 5-aminolevulinic acid aqueous solution at a temperature of 40-60° C. and a concentration of 60-80 wt % according to a mass volume ratio of 1 g: 10-20 mL, a silane coupling agent was added and stirred for 20-60 min, and then allowed to stand for 1-4 h to obtain a 5-aminolevulinic acid-loaded modified nano zeolite dispersion.

[0016] In a preferred embodiment of the present invention, the alkaline solution is an aqueous solution of sodium hydroxide and / or potassium hydroxide with a concentration of 30-50 wt%.

[0017] In a preferred embodiment of the present invention, the zeolite is selected from any one of clinoptilolite, mordenite, analcime and chabazite.

[0018] In a preferred embodiment of the present invention, the acidic solution is selected from an aqueous solution of any one of hydrochloric acid, nitric acid, citric acid, acetic acid, and succinic acid.

[0019] In a preferred embodiment of the present invention, the acidic amino acid is glutamic acid and / or aspartic acid.

[0020] In a preferred embodiment of the present invention, the amount of the acidic amino acid added is 0.5 to 1.5 times the mass of the cyclodextrin-coated zeolite.

[0021] In a preferred embodiment of the present invention, the stabilizer is polyvinyl pyrrolidone.

[0022] In a preferred embodiment of the present invention, the amount of the stabilizer added is 0.1 to 0.5 times the mass of the cyclodextrin-coated zeolite.

[0023] In a preferred embodiment of the present invention, the silane coupling agent is selected from any one of 4-mercaptobutyltrimethoxysilane, 4-mercaptobutyltriethoxysilane, 4-mercaptobutyltripropoxysilane, 4-mercaptobutylmethyldimethoxysilane and 4-mercaptobutylmethyldiethoxysilane.

[0024] In a preferred embodiment of the present invention, the amount of the silane coupling agent added is 0.03 to 0.05 times the mass of the modified nano-boiler.

[0025] In a preferred embodiment of the present invention, in step S3, the nutrient soil is composed of 80-120 parts of garden soil, 20-40 parts of wood ash, 10-40 parts of rapeseed cake, 5-10 parts of humus soil, and 20-40 parts of soybean residue powder in parts by weight.

[0026] In a preferred embodiment of the present invention, in step S4, the nutrient solution consists of: 200-400 mg / L potassium sulfate, 100-300 mg / L potassium nitrate, 50-100 mg / L calcium chloride, 100-150 mg / L potassium dihydrogen phosphate, 200-500 mg / L urea, 200-300 mg / L calcium sulfate, 100-200 mg / L sodium chloride, 50-100 mg / L zinc sulfate, 20-60 mg / L manganese sulfate, 10-20 mg / L ferrous sulfate, 2-4 mg / L boric acid and water.

[0027] By means of the above technical solution, the present invention has at least the following advantages:

[0028] 1. The present invention provides a method for raising seedlings of Prunus armeniaca, which promotes the rooting and germination of Prunus armeniaca leaf tissue explants by preparing a suitable germination induction culture medium, nutrient soil and nutrient solution, so that the seedlings are neat, strong and have a well-developed root system, and the survival rate of transplantation is high, thereby further improving the germination rate of Prunus armeniaca, reducing the seedling raising period and seedling raising cost of Prunus armeniaca, and reducing the investment in manpower and material resources for planting.

[0029] 2. The germination induction medium of the present invention uses 5-aminolevulinic acid as the main active substance. 5-aminolevulinic acid has the effects of promoting plant tissue differentiation, inhibiting breathing in the dark, expanding stomata and other basic physiological activities. By loading modified nano zeolites, the dormancy characteristics of leaf tissues are broken, and the air permeability and permeability of leaf tissues are increased. By loading modified nano zeolites, the germination and growth of 5-aminolevulinic acid on the leaf tissues of Prunus mume are improved, the seedling cultivation time is shortened, and the effects of microorganisms during the immersion of leaf tissues are inhibited, the activity of leaf tissues is ensured, and the germination rate of leaf tissues is improved.

[0030] 3. The present invention further provides a compounded nutrient soil and nutrient solution, thereby providing more nutrient sources for leaf tissue germination, improving the leaf tissue survival rate of the five-fingered peach, and further promoting leaf tissue germination and growth. The method of the present invention is simple and easy to operate, and the obtained seedlings have excellent growth and high survival rate after transplanting, and are suitable for large-scale planting.

[0031] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention and implement it according to the contents of the specification, the preferred embodiments of the present invention are described in detail as follows. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 : Average plant height of seedlings in each group at different time. DETAILED DESCRIPTION

[0033] In order to make the technical means, creative features, objectives and effects of the present invention easy to understand, the technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] Unless otherwise specified, the percentage content involved in the present invention refers to mass percentage for solid-liquid mixing and solid-solid mixing, and refers to volume percentage for liquid-liquid mixing.

[0035] Unless otherwise specified, the percentage concentrations referred to in the present invention all refer to final concentrations, which refer to the percentage of the added component in the system after the addition of the component.

[0036] The temperature parameters in the present invention, if not specifically limited, allow for both constant temperature treatment and treatment within a certain temperature range. The constant temperature treatment allows the temperature to fluctuate within the precision range controlled by the instrument.

[0037] Unless otherwise specified, the nutrient solution used in the following examples consists of: 300 mg / L potassium sulfate, 200 mg / L potassium nitrate, 75 mg / L calcium chloride, 125 mg / L potassium dihydrogen phosphate, 350 mg / L urea, 250 mg / L calcium sulfate, 150 mg / L sodium chloride, 75 mg / L zinc sulfate, 40 mg / L manganese sulfate, 15 mg / L ferrous sulfate, 3 mg / L boric acid and water.

[0038] Soybean residue powder was purchased from Linqu County Yongduo Feed Factory; rapeseed cake was purchased from Huama (Shandong) Biotechnology Co., Ltd.; humus soil was purchased from Xintai Wotian Ecological Agriculture Development Center; wood ash was purchased from Hebei Xiong'an Xinying Technology Co., Ltd.; zeolite was purchased from Shijiazhuang Chengruo Mineral Products Co., Ltd.

[0039] Unless otherwise specified, the experimental methods used in the following examples are all conventional methods.

[0040] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0041] Example 1

[0042] Preparation of germination induction medium: dissolving cyclodextrin in a sodium hydroxide solution with a concentration of 40wt% to obtain a cyclodextrin / sodium hydroxide solution with a concentration of 30wt%; then dispersing zeolite with an average particle size of 80 meshes in the cyclodextrin / sodium hydroxide solution according to a mass volume ratio of 1g:15mL and soaking for 10h, then taking it out to obtain cyclodextrin-coated zeolite. The obtained cyclodextrin-coated zeolite is placed in a 15wt% hydrochloric acid solution at a mass volume ratio of 1g:20mL and stirred continuously at 55°C for 2h, then cooled to room temperature, aspartic acid (added amount is 1.0 times the mass of the cyclodextrin-coated zeolite) and polyvinyl pyrrolidone (added amount is 0.3 times the mass of the cyclodextrin-coated zeolite) are added and stirred for 6h, then filtered and washed, dried and crushed through a 900-mesh sieve to obtain a modified nano zeolite. The modified nano zeolite was ultrasonically dispersed in a 50°C, 70wt% 5-aminolevulinic acid aqueous solution at a mass volume ratio of 1g:15mL, 4-mercaptobutyltrimethoxysilane was added (the amount added was 0.04 times the mass of the modified nano zeolite) and stirred for 40 minutes, and then allowed to stand for 2.5 hours to obtain a 5-aminolevulinic acid-loaded modified nano zeolite dispersion, which was the germination induction medium.

[0043] Preparation of nutrient soil: According to weight proportions, mix 100 parts of garden soil, 30 parts of wood ash, 25 parts of rapeseed cake, 7.5 parts of humus soil and 30 parts of soybean residue powder and stir well.

[0044] Seedling raising method of five-fingered peach: First, select 100 pieces of five-fingered peach leaf tissues of the same size as explants. Put them into a sterilized beaker and disinfect them with a 5% sodium hypochlorite aqueous solution for 10 minutes. After disinfection, rinse them with deionized water 5 times. Then dry them for use. The five-fingered peach leaf tissue is placed in a 20% volume concentration of germination induction medium aqueous solution at room temperature and a light intensity of 1500lux and soaked for 16 hours. The germination induction medium is a 5-aminolevulinic acid-loaded modified nano zeolite dispersion. The soaked leaf tissue is placed on a seedbed covered with nutrient soil according to the number of 2 leaf tissues per hole for cultivation, and water is sprayed until the soil is moistened. After 15 days of leaf tissue culture, the nutrient solution is sprayed at a frequency of every 5 days / time, and each spray is applied until the soil is moistened. During the seedling raising period, pests and diseases are controlled according to the normal process. When 90% of the seedlings are more than 10cm high, they are transplanted to the field. The germination of leaf tissue is counted, and the results are shown in Table 1.

[0045] Example 2

[0046] Preparation of germination induction medium: dissolving cyclodextrin in a sodium hydroxide solution with a concentration of 50wt% to obtain a cyclodextrin / sodium hydroxide solution with a concentration of 20wt%; then dispersing zeolite with an average particle size of 80 meshes in the cyclodextrin / sodium hydroxide solution according to a mass volume ratio of 1g:20mL and soaking for 8h, then taking it out to obtain cyclodextrin-coated zeolite. The obtained cyclodextrin-coated zeolite is placed in a 10wt% nitric acid solution at a mass volume ratio of 1g:30mL and stirred continuously at 70°C for 1h, then cooled to room temperature and added with glutamic acid (the amount added is 1.5 times the mass of the cyclodextrin-coated zeolite) and polyvinyl pyrrolidone (the amount added is 0.5 times the mass of the cyclodextrin-coated zeolite) and stirred for 8h, then allowed to stand and filtered, and the obtained product is washed, dried, and crushed through a 1000-mesh sieve to obtain a modified nano zeolite. The modified nano zeolite was ultrasonically dispersed in a 5-aminolevulinic acid aqueous solution at 60°C and a concentration of 80wt% according to a mass-to-volume ratio of 1g:10mL, 4-mercaptobutyltriethoxysilane was added (the amount added was 0.05 times the mass of the modified nano zeolite) and stirred for 20 minutes, and then allowed to stand for 4 hours to obtain a 5-aminolevulinic acid-loaded modified nano zeolite dispersion, which was the germination induction medium.

[0047] Preparation of nutrient soil: According to weight proportions, mix 80 parts of garden soil, 40 parts of wood ash, 40 parts of rapeseed cake, 10 parts of humus soil, and 40 parts of soybean residue powder and stir well.

[0048] Seedling raising method of five-fingered peach: First, select 100 pieces of five-fingered peach leaf tissues of the same size as explants. Put them into a sterilized beaker and disinfect them with a 5% sodium hypochlorite aqueous solution for 10 minutes. After disinfection, rinse them with deionized water 5 times. Then dry them for standby use. Soak the five-fingered peach leaf tissue in a 30% volume concentration of germination induction medium aqueous solution at room temperature and a light intensity of 1500lux for 12 hours. The germination induction medium is a 5-aminolevulinic acid-loaded modified nano zeolite dispersion. The soaked leaf tissue is placed on a seedbed covered with nutrient soil according to the number of 2 leaf tissues per hole for cultivation, and water is sprayed until the soil is moistened. After 15 days of leaf tissue culture, spray the nutrient solution at a frequency of every 5 days / time, and spray until the soil is moistened each time. During the seedling raising period, pests and diseases are controlled according to the normal process. When 90% of the seedlings are more than 10cm high, they are transplanted to the field. The germination of leaf tissue is counted, and the results are shown in Table 1.

[0049] Example 3

[0050] Preparation of germination induction medium: dissolving cyclodextrin in a sodium hydroxide solution with a concentration of 30wt% to obtain a cyclodextrin / sodium hydroxide solution with a concentration of 40wt%; then dispersing zeolite with an average particle size of 80 meshes in the cyclodextrin / sodium hydroxide solution according to a mass volume ratio of 1g:10mL and soaking for 12h, then taking it out to obtain cyclodextrin-coated zeolite. The obtained cyclodextrin-coated zeolite is placed in a 20wt% citric acid solution at a mass volume ratio of 1g:10mL and stirred for 3h at 40°C, then cooled to room temperature, aspartic acid (the amount added is 0.5 times the mass of the cyclodextrin-coated zeolite) and polyvinyl pyrrolidone (the amount added is 0.1 times the mass of the cyclodextrin-coated zeolite) are added and stirred for 4h, then filtered and washed, dried and crushed through an 800-mesh sieve to obtain a modified nano zeolite. The modified nano zeolite was ultrasonically dispersed in a 60wt% 5-aminolevulinic acid aqueous solution at 40°C according to a mass-to-volume ratio of 1g:20mL, 4-mercaptobutyltripropoxysilane was added (the amount added was 0.03 times the mass of the modified nano zeolite) and stirred for 60min, and then allowed to stand for 1h to obtain a 5-aminolevulinic acid-loaded modified nano zeolite dispersion, which was the germination induction medium.

[0051] Preparation of nutrient soil: According to weight proportions, mix 120 parts of garden soil, 20 parts of wood ash, 10 parts of rapeseed cake, 5 parts of humus soil, and 20 parts of soybean residue powder and stir well.

[0052] Seedling raising method of five-fingered peach: First, select 100 pieces of five-fingered peach leaf tissues of the same size as explants. Put them into a sterilized beaker and disinfect them with a 5% sodium hypochlorite aqueous solution for 10 minutes. After disinfection, rinse them with deionized water 5 times. Then dry them for use. The five-fingered peach leaf tissue is placed in a 10% volume concentration of germination induction medium aqueous solution at room temperature and a light intensity of 1500lux and soaked for 20 hours. The germination induction medium is a 5-aminolevulinic acid-loaded modified nano zeolite dispersion. The soaked leaf tissue is placed on a seedbed covered with nutrient soil according to the number of 2 leaf tissues per hole for cultivation, and water is sprayed until the soil is moistened. After 15 days of leaf tissue culture, the nutrient solution is sprayed at a frequency of every 5 days / time, and each spray is applied until the soil is moistened. During the seedling raising period, pests and diseases are controlled according to the normal process. When 90% of the seedlings are more than 10cm high, they are transplanted to the field. The germination of leaf tissue is counted, and the results are shown in Table 1.

[0053] Comparative Example 1

[0054] Preparation of germination induction medium: Place zeolite with an average particle size of 80 mesh in a 15wt% hydrochloric acid solution at a mass volume ratio of 1g:20mL and stir continuously at 55℃ for 2h, then add aspartic acid (added amount is 1.0 times the mass of zeolite) and polyvinylpyrrolidone (added amount is 0.3 times the mass of zeolite) after cooling to room temperature, stir and react for 6h, then stand and filter, wash, dry and crush the obtained product through a 900 mesh sieve to obtain modified nano zeolite. Ultrasonic disperse the obtained modified nano zeolite in a 50℃, 70wt% 5-aminolevulinic acid aqueous solution at a mass volume ratio of 1g:15mL, add 4-mercaptobutyltrimethoxysilane (added amount is 0.04 times the mass of modified nano zeolite) and stir for 40min, then stand for 2.5h to obtain a 5-aminolevulinic acid-loaded modified nano zeolite dispersion, which is the germination induction medium.

[0055] Preparation of nutrient soil: According to weight proportions, mix 100 parts of garden soil, 30 parts of wood ash, 25 parts of rapeseed cake, 7.5 parts of humus soil and 30 parts of soybean residue powder and stir well.

[0056] Seedling raising method of five-fingered peach: First, select 100 pieces of five-fingered peach leaf tissues of the same size as explants. Put them into a sterilized beaker and disinfect them with a 5% sodium hypochlorite aqueous solution for 10 minutes. After disinfection, rinse them with deionized water 5 times. Then dry them for use. The five-fingered peach leaf tissue is placed in a 20% volume concentration of germination induction medium aqueous solution at room temperature and a light intensity of 1500lux and soaked for 16 hours. The germination induction medium is a 5-aminolevulinic acid-loaded modified nano zeolite dispersion. The soaked leaf tissue is placed on a seedbed covered with nutrient soil according to the number of 2 leaf tissues per hole for cultivation, and water is sprayed until the soil is moistened. After 15 days of leaf tissue culture, the nutrient solution is sprayed at a frequency of every 5 days / time, and each spray is applied until the soil is moistened. During the seedling raising period, pests and diseases are controlled according to the normal process. When 90% of the seedlings are more than 10cm high, they are transplanted to the field. The germination of leaf tissue is counted, and the results are shown in Table 1.

[0057] Comparative Example 2

[0058] Preparation of germination induction medium: dissolving cyclodextrin in a sodium hydroxide solution with a concentration of 40wt% to obtain a cyclodextrin / sodium hydroxide solution with a concentration of 30wt%; then dispersing a zeolite with an average particle size of 80 meshes in the cyclodextrin / sodium hydroxide solution according to a mass volume ratio of 1g:15mL and soaking for 10h, then taking out, washing, drying and crushing the obtained product through a 900 mesh sieve to obtain a modified nano zeolite. Ultrasonic dispersion of the obtained modified nano zeolite in a 50°C, 70wt% 5-aminolevulinic acid aqueous solution according to a mass volume ratio of 1g:15mL, adding 4-mercaptobutyltrimethoxysilane (the amount added is 0.04 times the mass of the modified nano zeolite) and stirring for 40min, then standing for 2.5h to obtain a 5-aminolevulinic acid-loaded modified nano zeolite dispersion, which is a germination induction medium.

[0059] Preparation of nutrient soil: According to weight proportions, mix 100 parts of garden soil, 30 parts of wood ash, 25 parts of rapeseed cake, 7.5 parts of humus soil and 30 parts of soybean residue powder and stir well.

[0060] Seedling raising method of five-fingered peach: First, select 100 pieces of five-fingered peach leaf tissues of the same size as explants. Put them into a sterilized beaker and disinfect them with a 5% sodium hypochlorite aqueous solution for 10 minutes. After disinfection, rinse them with deionized water 5 times. Then dry them for use. The five-fingered peach leaf tissue is placed in a 20% volume concentration of germination induction medium aqueous solution at room temperature and a light intensity of 1500lux and soaked for 16 hours. The germination induction medium is a 5-aminolevulinic acid-loaded modified nano zeolite dispersion. The soaked leaf tissue is placed on a seedbed covered with nutrient soil according to the number of 2 leaf tissues per hole for cultivation, and water is sprayed until the soil is moistened. After 15 days of leaf tissue culture, the nutrient solution is sprayed at a frequency of every 5 days / time, and each spray is applied until the soil is moistened. During the seedling raising period, pests and diseases are controlled according to the normal process. When 90% of the seedlings are more than 10cm high, they are transplanted to the field. The germination of leaf tissue is counted, and the results are shown in Table 1.

[0061] Comparative Example 3

[0062] Preparation of germination induction medium: ultrasonically disperse zeolite with an average particle size of 900 mesh in a 50°C, 70wt% 5-aminolevulinic acid aqueous solution at a mass volume ratio of 1g:15mL, add 4-mercaptobutyltrimethoxysilane (the amount added is 0.04 times the mass of zeolite) and stir for 40 minutes, then let it stand for 2.5 hours to obtain a 5-aminolevulinic acid-loaded nanozeolite dispersion, which is the germination induction medium.

[0063] Preparation of nutrient soil: According to weight proportions, mix 100 parts of garden soil, 30 parts of wood ash, 25 parts of rapeseed cake, 7.5 parts of humus soil and 30 parts of soybean residue powder and stir well.

[0064] Seedling raising method of five-fingered peach: First, select 100 pieces of five-fingered peach leaf tissues of the same size as explants. Put them into a sterilized beaker and disinfect them with a 5% sodium hypochlorite aqueous solution for 10 minutes. After disinfection, rinse them with deionized water 5 times. Then dry them for use. The five-fingered peach leaf tissue is placed in a 20% volume concentration of germination induction medium aqueous solution at room temperature and a light intensity of 1500lux and soaked for 16 hours. The germination induction medium is a 5-aminolevulinic acid-loaded modified nano zeolite dispersion. The soaked leaf tissue is placed on a seedbed covered with nutrient soil according to the number of 2 leaf tissues per hole for cultivation, and water is sprayed until the soil is moistened. After 15 days of leaf tissue culture, the nutrient solution is sprayed at a frequency of every 5 days / time, and each spray is applied until the soil is moistened. During the seedling raising period, pests and diseases are controlled according to the normal process. When 90% of the seedlings are more than 10cm high, they are transplanted to the field. The germination of leaf tissue is counted, and the results are shown in Table 1.

[0065] Comparative Example 4

[0066] Preparation of germination-promoting induction medium: Prepare a 70wt% 5-aminolevulinic acid aqueous solution, which is the germination-promoting induction medium.

[0067] Preparation of nutrient soil: According to weight proportions, mix 100 parts of garden soil, 30 parts of wood ash, 25 parts of rapeseed cake, 7.5 parts of humus soil and 30 parts of soybean residue powder and stir well.

[0068] Seedling raising method of five-fingered peach: First, select 100 pieces of five-fingered peach leaf tissues of the same size as explants. Put them into a sterilized beaker and disinfect them with a 5% sodium hypochlorite aqueous solution for 10 minutes. After disinfection, rinse them with deionized water 5 times. Then dry them for use. The five-fingered peach leaf tissue is placed in a 20% volume concentration of germination induction medium aqueous solution at room temperature and a light intensity of 1500lux and soaked for 16 hours. The germination induction medium is a 5-aminolevulinic acid-loaded modified nano zeolite dispersion. The soaked leaf tissue is placed on a seedbed covered with nutrient soil according to the number of 2 leaf tissues per hole for cultivation, and water is sprayed until the soil is moistened. After 15 days of leaf tissue culture, the nutrient solution is sprayed at a frequency of every 5 days / time, and each spray is applied until the soil is moistened. During the seedling raising period, pests and diseases are controlled according to the normal process. When 90% of the seedlings are more than 10cm high, they are transplanted to the field. The germination of leaf tissue is counted, and the results are shown in Table 1.

[0069] Comparative Example 5

[0070] Preparation of nutrient soil: According to weight proportions, mix 100 parts of garden soil, 30 parts of wood ash, 25 parts of rapeseed cake, 7.5 parts of humus soil and 30 parts of soybean residue powder and stir well.

[0071] Seedling raising method of Prunus armeniaca: First, select 100 pieces of Prunus armeniaca leaf tissues of the same size as explants. Put them into a sterilized beaker and disinfect them with a 5% sodium hypochlorite aqueous solution for 10 minutes. After disinfection, rinse them with deionized water 5 times. Then dry them for later use. Soak the Prunus armeniaca leaf tissue in water at room temperature for 16 hours. Place the soaked leaf tissues on a seedbed covered with nutrient soil according to the number of 2 leaf tissues per hole for cultivation, and spray water until the soil is moist. After 15 days of leaf tissue culture, spray the nutrient solution at a frequency of every 5 days, and spray until the soil is moist each time. During the seedling raising period, carry out disease and insect pest control according to the normal process. When 90% of the seedlings are more than 10 cm tall, they can be transplanted to the field. The germination of leaf tissue was counted, and the results are shown in Table 1.

[0072] Comparative Example 6

[0073] Preparation of germination induction medium: dissolving cyclodextrin in a sodium hydroxide solution with a concentration of 40wt% to obtain a cyclodextrin / sodium hydroxide solution with a concentration of 30wt%; then dispersing zeolite with an average particle size of 80 meshes in the cyclodextrin / sodium hydroxide solution according to a mass volume ratio of 1g:15mL and soaking for 10h, then taking it out to obtain cyclodextrin-coated zeolite. The obtained cyclodextrin-coated zeolite is placed in a 15wt% hydrochloric acid solution at a mass volume ratio of 1g:20mL and stirred continuously at 55°C for 2h, then cooled to room temperature, aspartic acid (added amount is 1.0 times the mass of the cyclodextrin-coated zeolite) and polyvinyl pyrrolidone (added amount is 0.3 times the mass of the cyclodextrin-coated zeolite) are added and stirred for 6h, then filtered and washed, dried and crushed through a 900-mesh sieve to obtain a modified nano zeolite. The modified nano zeolite was ultrasonically dispersed in a 50°C, 70wt% 5-aminolevulinic acid aqueous solution at a mass volume ratio of 1g:15mL, 4-mercaptobutyltrimethoxysilane was added (the amount added was 0.04 times the mass of the modified nano zeolite) and stirred for 40 minutes, and then allowed to stand for 2.5 hours to obtain a 5-aminolevulinic acid-loaded modified nano zeolite dispersion, which was the germination induction medium.

[0074] Preparation of nutrient soil: According to weight proportions, mix 130 parts of garden soil, 30 parts of wood ash, 25 parts of rapeseed cake, and 7.5 parts of humus soil and stir evenly.

[0075] Seedling raising method of five-fingered peach: First, select 100 pieces of five-fingered peach leaf tissues of the same size as explants. Put them into a sterilized beaker and disinfect them with a 5% sodium hypochlorite aqueous solution for 10 minutes. After disinfection, rinse them with deionized water 5 times. Then dry them for use. The five-fingered peach leaf tissue is placed in a 20% volume concentration of germination induction medium aqueous solution at room temperature and a light intensity of 1500lux and soaked for 16 hours. The germination induction medium is a 5-aminolevulinic acid-loaded modified nano zeolite dispersion. The soaked leaf tissue is placed on a seedbed covered with nutrient soil according to the number of 2 leaf tissues per hole for cultivation, and water is sprayed until the soil is moistened. After 15 days of leaf tissue culture, the nutrient solution is sprayed at a frequency of every 5 days / time, and each spray is applied until the soil is moistened. During the seedling raising period, pests and diseases are controlled according to the normal process. When 90% of the seedlings are more than 10cm high, they are transplanted to the field. The germination of leaf tissue is counted, and the results are shown in Table 1.

[0076] Germination rate = number of germinations at time t / total number of leaf tissues × 100%;

[0077] Average seedling height = total height of all seedlings / number of seedlings.

[0078] Table 1 Statistics of germination of five-fingered peaches in different embodiments

[0079] Group Number of germinations / block Germination rate / % Average seedling height / cm Example 1 99 99 12.7 Example 2 97 97 12.3 Example 3 99 99 12.9 Comparative Example 1 95 95 12.1 Comparative Example 2 93 93 12.2 Comparative Example 3 90 90 11.8 Comparative Example 4 88 88 11.4 Comparative Example 5 83 83 10.2 Comparative Example 6 96 96 11.9

[0080] It can be seen from the results in Table 1 that the germination rate of Prunus quinquefolia can be significantly improved by adding a germination-promoting induction medium in the seedling raising method of the present invention, and at the same time, the obtained seedlings have uniform growth and the seedling height is suitable for transplanting.

[0081] Experiment 1: Survival and growth of transplanted seedlings

[0082] 100 seedlings with substantially the same growth potential obtained by the cultivation methods of Example 1 and Comparative Examples 1 to 6 were selected as seedlings for transplanting.

[0083] A large field of fruit and vegetable planting base was selected and divided into 7 experimental fields according to their area size, which were numbered 1 to 7.

[0084] The seedlings of Example 1 and Comparative Examples 1 to 6 were transplanted to test fields 1 to 7, respectively. After 15 days, the number of surviving seedlings in each test field was counted and the survival rate was calculated. The results are shown in Table 2. At the same time, the seedling height was counted every 10 days, and the total count was 60 days. The results are shown in Table 2. Figure 1 During the planting period, each experimental field maintained the same field management mode.

[0085] Table 2 Seedling survival rate in different experimental fields

[0086] Group Survival rate / % Example 1 99 Comparative Example 1 97 Comparative Example 2 97 Comparative Example 3 95 Comparative Example 4 93 Comparative Example 5 87 Comparative Example 6 96

[0087] It can be seen from the results in Table 2 that the survival rates of seedlings in different test fields are not much different, among which the seedlings in Comparative Example 5 have the lowest survival rate, indicating that the germination induction medium of the present invention can also affect the survival rate of seedlings to a certain extent.

[0088] Depend on Figure 1 It can be seen from the results that the plant height of the seedlings in different test fields showed an increasing trend as time went on within the statistical period of 60 days, and the seedlings in comparative example 5 grew the slowest, indicating that the germination induction medium of the present invention can affect the growth rate of seedlings to a certain extent.

[0089] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technician familiar with this profession can make some changes or modifications to equivalent embodiments of equivalent changes by using the methods and technical contents disclosed above without departing from the scope of the technical solution of the present invention. However, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A method for raising seedlings of Prunus mume, characterized in that: The method is: S1: Wash and disinfect the leaves of the Eleutherodendron chinense, dry them in the air and set them aside; S2: The leaf tissue of Prunus armeniaca was placed as an explant in a germination induction medium and immersed at room temperature for 12 to 20 hours. The germination induction medium was a dispersion of 5-aminolevulinic acid-loaded modified nano-zeolite. S3: The soaked leaf tissues are placed on a seedbed covered with nutrient soil at a quantity of 1 to 2 leaf tissues per hole for cultivation, and water is sprayed until the soil is moistened; S4: After 10-20 days of leaf tissue culture, spray the nutrient solution every 3-5 days until the soil is moistened. When the seedlings are cultured to a height of 10-15 cm, transplant them to the field. The preparation method of the germination induction medium comprises: Dissolve cyclodextrin in an alkaline solution to obtain a cyclodextrin alkaline solution with a concentration of 20-40 wt%; disperse zeolite in the cyclodextrin alkaline solution according to a mass volume ratio of 1 g: 10-20 mL and soak for 8-12 hours, then take out to obtain cyclodextrin-coated zeolite; According to 1g: 10-30mL, cyclodextrin-coated zeolite is placed in a 10-20wt% acid solution at 40-70°C and stirred for 1-3h. After cooling to room temperature, acidic amino acid and polyvinyl pyrrolidone are added. The mixture is stirred for 4-8h, allowed to stand, filtered, and the obtained product is washed, dried, crushed, and sieved through an 800-1000 mesh sieve to obtain a modified nano zeolite. The modified nano-zeolite is ultrasonically dispersed in a 60-80 wt% 5-aminolevulinic acid aqueous solution at 40-60°C according to 1 g: 10-20 mL, a silane coupling agent is added, stirred for 20-60 min, and allowed to stand for 1-4 h to obtain a 5-aminolevulinic acid-loaded modified nano-zeolite dispersion; The silane coupling agent is selected from any one of 4-mercaptobutyltrimethoxysilane, 4-mercaptobutyltriethoxysilane, 4-mercaptobutyltripropoxysilane, 4-mercaptobutylmethyldimethoxysilane and 4-mercaptobutylmethyldiethoxysilane.

2. The method for raising seedlings of the five-fingered peach according to claim 1, characterized in that: The alkaline solution is an aqueous solution of sodium hydroxide and / or potassium hydroxide with a concentration of 30-50wt%.

3. The method for raising seedlings of the five-fingered peach according to claim 1, characterized in that: The zeolite is selected from any one of clinoptilolite, mordenite, analcime and chabazite.

4. The method for raising seedlings of Prunus armeniaca according to claim 1, characterized in that: The acidic solution is selected from any one of aqueous solutions of hydrochloric acid, nitric acid, citric acid, acetic acid, and succinic acid.

5. The method for raising seedlings of Prunus armeniaca according to claim 1, characterized in that: The acidic amino acid is glutamic acid and / or aspartic acid; the added amount of the acidic amino acid is 0.5 to 1.5 times the mass of the cyclodextrin-coated zeolite.

6. The method for raising seedlings of Prunus armeniaca according to claim 1, characterized in that: The amount of polyvinyl pyrrolidone added is 0.1 to 0.5 times the mass of the cyclodextrin-coated zeolite.

7. The method for raising seedlings of Prunus armeniaca according to claim 1, characterized in that: The amount of silane coupling agent added is 0.03-0.05 times the mass of the modified nano-boiling point.

8. The method for raising seedlings of Prunus armeniaca according to claim 1, characterized in that: In step S3, the nutrient soil is composed of 80-120 parts of garden soil, 20-40 parts of wood ash, 10-40 parts of rapeseed cake, 5-10 parts of humus soil, and 20-40 parts of soybean residue powder in parts by weight.

9. The method for raising seedlings of Prunus armeniaca according to claim 1, characterized in that: In step S4, the nutrient solution consists of: 200-400 mg / L potassium sulfate, 100-300 mg / L potassium nitrate, 50-100 mg / L calcium chloride, 100-150 mg / L potassium dihydrogen phosphate, 200-500 mg / L urea, 200-300 mg / L calcium sulfate, 100-200 mg / L sodium chloride, 50-100 mg / L zinc sulfate, 20-60 mg / L manganese sulfate, 10-20 mg / L ferrous sulfate, 2-4 mg / L boric acid and water.

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