A Chinese medicine extract and its preparation and application

Abstract

The present invention provides a Chinese medicine extract and its preparation and application. The Chinese medicine extract of the present invention is obtained by water extraction from a Chinese medicine composition; the Chinese medicine composition comprises the following components in parts by weight: 15-20 parts of Sophora flavescens, 25-30 parts of Phellodendron chinense, 15-20 parts of Cnidium monnieri, 15-20 parts of Kochia scoparia, 8-12 parts of Stemona, 8-12 parts of Lithospermum officinale, 8-12 parts of Atractylodes macrocephala, 12-20 parts of Leonurus japonicus, 12-20 parts of Honeysuckle, and 8-12 parts of Saussurea involucrata. The present invention adopts specific Chinese medicine ingredients for compounding, and the obtained Chinese medicine composition can achieve simultaneous inhibition of common pathogenic microorganisms (Escherichia coli, Staphylococcus aureus, Candida albicans, Trichomonas) in the vagina after water extraction, and compared with the products obtained by water extraction of other Chinese medicine ingredients, it plays a more excellent role in inhibiting pathogenic microorganisms, and is more suitable for preparing drugs for treating vaginitis.

Description

Technical Field

[0001] The present invention belongs to the technical field of gel, and more specifically, relates to a Chinese medicine extract and its preparation and application. Background Art

[0002] Vaginitis is a group of diseases that cause vulvovaginal itching, burning pain, irritation and abnormal fluid discharge, affecting women's physical and mental health. Its onset is often related to the imbalance of the patient's vaginal microecology, which is mainly reflected in the massive reproduction of pathogenic microorganisms (such as Escherichia coli, Staphylococcus aureus, Candida albicans, Trichomonas, etc.), which brings great inconvenience to the patient's normal life and work. Common types of vaginitis include Escherichia coli vaginitis, Staphylococcus aureus vaginitis, Candida albicans vaginitis and Trichomonas vaginitis, but patients with various types of vaginitis are often seen clinically. This is because the presence of many pathogenic microorganisms may destroy the microecological balance of the vagina, thereby providing further opportunities for the invasion of other pathogenic microorganisms. Therefore, it is very important to find a product that can more widely inhibit common pathogenic microorganisms in the vagina for the treatment of vaginitis.

[0003] At present, the treatment methods for Escherichia coli vaginitis and Staphylococcus aureus vaginitis are usually metronidazole, cephalosporins, etc., the treatment methods for Candida albicans vaginitis are usually clotrimazole, fluconazole, etc., and the treatment methods for Trichomonas vaginitis are usually metronidazole, tinidazole, etc. However, these drugs are all antibiotics, and long-term use is likely to cause pathogenic microorganisms to develop drug resistance, and are not suitable for long-term treatment of vaginitis. Therefore, traditional Chinese medicine products with high safety factors and low resistance have gradually stood out among many vaginitis treatment products. However, there are many single traditional Chinese medicines that have the effect of inhibiting pathogenic microorganisms, and traditional Chinese medicine compounds with different ingredients have obvious differences, and the efficacy cannot be predicted. Therefore, it is not easy to obtain a traditional Chinese medicine product that can simultaneously inhibit common pathogenic microorganisms in the vagina. Summary of the invention

[0004] The present invention aims to provide a Chinese medicine extract in view of the deficiencies in the prior art. By compounding Chinese medicines with specific ingredients, the extract can simultaneously inhibit common pathogenic microorganisms in the vagina (Escherichia coli, Staphylococcus aureus, Candida albicans, Trichomonas), and is very suitable for preparing drugs for treating vaginitis.

[0005] The first object of the present invention is to provide a Chinese herbal medicine extract.

[0006] A second object of the present invention is to provide a gel.

[0007] The third object of the present invention is to provide the use of the above-mentioned Chinese medicine extract or the above-mentioned gel in the preparation of products that inhibit pathogenic microorganisms.

[0008] The fourth object of the present invention is to provide the use of the above-mentioned Chinese medicine extract or the above-mentioned gel in the preparation of a medicine for treating vaginitis.

[0009] The fifth object of the present invention is to provide the use of the above gel in the preparation of vaginal mucosal repair products.

[0010] The above-mentioned purpose of the present invention is achieved through the following technical solutions:

[0011] The invention provides a traditional Chinese medicine extract, which is obtained by water extraction from a traditional Chinese medicine composition; the traditional Chinese medicine composition comprises the following components in parts by weight: 15-20 parts of sophora flavescens, 25-30 parts of phellodendron amurense, 15-20 parts of cnidium monnieri, 15-20 parts of kochia scoparia, 8-12 parts of stemonae, 8-12 parts of lithospermum officinale, 8-12 parts of atractylodes, 12-20 parts of leonurus, 12-20 parts of honeysuckle and 8-12 parts of saussurea involucrata.

[0012] Preferably, the water extraction is carried out at 95-100° C. for 1.5-2 h.

[0013] Preferably, the water is prepared in advance, and the Chinese medicine composition is first soaked in water for 8 to 10 hours.

[0014] Further preferably, during the soaking, the mass ratio of the traditional Chinese medicine composition to water is 1:8-10.

[0015] Preferably, after the water extraction, filtration and concentration are further performed.

[0016] More preferably, the concentration is to concentrate the filtrate at 40-60° C. for 2-4 hours.

[0017] Based on this, the present invention also provides a gel having a pH of 4.5 to 6, and the gel comprises the following components in parts by weight: 40 to 50 parts of the above-mentioned traditional Chinese medicine extract, 20 to 30 parts of gel matrix, and 100 parts of water.

[0018] Preferably, the gel matrix comprises the following components in parts by weight: 1.8-2.2 parts of carbomer, 0.5-1 part of hyaluronic acid, and water to make up to 100 parts.

[0019] Further preferably, the gel matrix is ​​obtained by mixing the components in the formula amounts until transparent.

[0020] Preferably, the pH is adjusted by triethanolamine.

[0021] Preferably, the pH is 4.5-6, and the gel comprises the following components in parts by weight: 40-50 parts of the above-mentioned traditional Chinese medicine extract, 20-30 parts of gel matrix, 0.02-0.3 parts of chlorhexidine acetate, and water to make up to 100 parts.

[0022] Preferably, the pH is 4.5-6, and the gel comprises the following components in parts by weight: 40-50 parts of the above-mentioned Chinese medicine extract, 20-30 parts of gel matrix, 0.02-0.3 parts of chlorhexidine acetate, 1-2 parts of propolis, and water to make up to 100 parts. Adding propolis to the gel can significantly enhance the nourishing and repairing effect of the gel on the vaginal mucosa.

[0023] Preferably, the pH is 4.5-6, and the gel comprises the following components in parts by weight: 40-50 parts of the above-mentioned Chinese herbal extract, 20-30 parts of gel matrix, 0.02-0.3 parts of chlorhexidine acetate, 0.2-0.5 parts of vitamin E, and water to make up to 100 parts. Adding vitamin E to the gel can significantly enhance the nourishing and repairing effect of the gel on the vaginal mucosa.

[0024] The present invention adopts specific Chinese medicine ingredients for compounding, and the obtained Chinese medicine composition, after water extraction, has a more excellent effect in inhibiting pathogenic microorganisms such as Escherichia coli, Staphylococcus aureus, Candida albicans, Trichomonas, etc., compared with the products obtained by water extraction of other Chinese medicine ingredients, and is more suitable for preparing products that inhibit pathogenic microorganisms and drugs for treating vaginitis. Therefore, the application of the above-mentioned Chinese medicine extract in the preparation of products that inhibit pathogenic microorganisms and drugs for treating vaginitis should be within the protection scope of the present invention, wherein the pathogenic microorganism is one or more of Escherichia coli, Staphylococcus aureus, Candida albicans, and Trichomonas; the vaginitis is one or more of Escherichia coli vaginitis, Staphylococcus aureus vaginitis, Candida albicans vaginitis, and Trichomonas vaginitis.

[0025] In addition, the above-mentioned gel can not only effectively inhibit pathogenic microorganisms, but also effectively repair vaginal mucosa. Therefore, the use of the above-mentioned gel in the preparation of products for inhibiting pathogenic microorganisms, drugs for treating vaginitis, and vaginal mucosa repair products should also be within the protection scope of the present invention, wherein the pathogenic microorganisms are one or more of Staphylococcus aureus, Candida albicans, Escherichia coli, and Trichomonas; the vaginitis is one or more of Escherichia coli vaginitis, Staphylococcus aureus vaginitis, Candida albicans vaginitis, and Trichomonas vaginitis.

[0026] Based on this, the present invention also provides a method for preparing the above-mentioned gel, namely, the gel is prepared by uniformly mixing the components in the formula amount.

[0027] The present invention has the following beneficial effects:

[0028] The invention adopts specific Chinese medicinal ingredients for compounding, and the obtained Chinese medicinal composition can achieve simultaneous inhibition of common pathogenic microorganisms (Escherichia coli, Staphylococcus aureus, Candida albicans, Trichomonas) in the vagina after water extraction, and compared with products obtained by water extraction of other Chinese medicinal ingredients, it plays a more excellent role in inhibiting pathogenic microorganisms such as Escherichia coli, Staphylococcus aureus, Candida albicans, Trichomonas and the like, and is more suitable for preparing drugs for treating vaginitis. DETAILED DESCRIPTION

[0029] The present invention is further described below with reference to specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0030] Unless otherwise specified, the reagents and materials used in the following examples are commercially available.

[0031] Example 1 Preparation of Chinese herbal medicine extract

[0032] 1. Ingredients of Chinese medicine composition

[0033] The invention comprises the following components in parts by weight: 18 parts of Sophora flavescens, 28 parts of Phellodendron amurense, 18 parts of Cnidium monnieri, 18 parts of Kochia scoparia, 10 parts of Stemona japonica, 10 parts of Lithospermum officinale, 10 parts of Atractylodes lancea, 18 parts of Leonurus japonicus, 18 parts of Honeysuckle, and 10 parts of Saussurea involucrata.

[0034] 2. Preparation method of Chinese herbal medicine extract

[0035] First, soak the Chinese medicine composition in water for 9 hours (the mass ratio of the Chinese medicine composition to water is 1:9), then heat it at 100°C for 1.8 hours, filter it, and concentrate the filtrate at 50°C for 3 hours to obtain the product.

[0036] Example 2 Preparation of Chinese herbal medicine extract

[0037] 1. Ingredients of Chinese medicine composition

[0038] The invention comprises the following components in parts by weight: 20 parts of Sophora flavescens, 30 parts of Phellodendron amurense, 15 parts of Cnidium monnieri, 20 parts of Kochia scoparia, 8 parts of Stemona japonica, 8 parts of Lithospermum officinale, 8 parts of Atractylodes lancea, 12 parts of Leonurus japonicus, 12 parts of Honeysuckle, and 8 parts of Saussurea involucrata.

[0039] 2. Preparation method of Chinese herbal medicine extract

[0040] First, soak the Chinese medicine composition in water for 10 hours (the mass ratio of the Chinese medicine composition to water is 1:8), then heat it at 100°C for 1.5 hours, filter it, and concentrate the filtrate at 40°C for 4 hours to obtain the product.

[0041] Example 3 Preparation of Chinese herbal medicine extract

[0042] 1. Ingredients of Chinese medicine composition

[0043] The invention comprises the following components in parts by weight: 15 parts of Sophora flavescens, 25 parts of Phellodendron amurense, 20 parts of Cnidium monnieri, 15 parts of Kochia scoparia, 12 parts of Stemona japonica, 12 parts of Lithospermum officinale, 12 parts of Atractylodes lancea, 20 parts of Leonurus japonicus, 20 parts of Honeysuckle, and 12 parts of Saussurea involucrata.

[0044] 2. Preparation method of Chinese herbal medicine extract

[0045] First, soak the Chinese medicine composition in water for 8 hours (the mass ratio of the Chinese medicine composition to water is 1:10), then heat it at 95°C for 2 hours, filter it, and concentrate the filtrate at 60°C for 2 hours.

[0046] Example 4 Preparation of gel

[0047] 1. Gel Matrix

[0048] (1)Ingredients

[0049] The composition comprises the following components in parts by weight: 2 parts of carbomer, 0.8 parts of hyaluronic acid, and water to make up to 100 parts.

[0050] (2) Preparation method

[0051] Mix the components in the formula until transparent.

[0052] 2. Gel

[0053] (1)Ingredients

[0054] The pH value is adjusted to 5.5 by triethanolamine, and the mixture comprises the following components in parts by weight: 45 parts of the Chinese herbal extract obtained in Example 1, 25 parts of the gel matrix, 0.15 parts of chlorhexidine acetate, and water to make up to 100 parts.

[0055] (2) Preparation method

[0056] Mix all the ingredients in the formula evenly.

[0057] Example 5 Preparation of gel

[0058] 1. Gel Matrix

[0059] (1)Ingredients

[0060] The composition comprises the following components in parts by weight: 1.8 parts of carbomer, 1 part of hyaluronic acid, and 100 parts of water.

[0061] (2) Preparation method

[0062] Mix the components in the formula until transparent.

[0063] 2. Gel

[0064] (1)Ingredients

[0065] The pH is adjusted to 4.5 by triethanolamine, and the mixture comprises the following components in parts by weight: 40 parts of the Chinese herbal extract obtained in Example 1, 20 parts of a gel matrix, 0.02 parts of chlorhexidine acetate, and water to make up to 100 parts.

[0066] (2) Preparation method

[0067] Mix all the ingredients in the formula evenly.

[0068] Example 6 Preparation of gel

[0069] 1. Gel Matrix

[0070] (1)Ingredients

[0071] The composition comprises the following components in parts by weight: 2.2 parts of carbomer, 0.5 parts of hyaluronic acid, and water to make up to 100 parts.

[0072] (2) Preparation method

[0073] Mix the components in the formula until transparent.

[0074] 2. Gel

[0075] (1)Ingredients

[0076] The pH is adjusted to 6 by triethanolamine, and the mixture contains the following components in parts by weight: 50 parts of the Chinese herbal extract obtained in Example 1, 30 parts of a gel matrix, 0.3 parts of chlorhexidine acetate, and water to make up to 100 parts.

[0077] (2) Preparation method

[0078] Mix all the ingredients in the formula evenly.

[0079] Example 7 Preparation of gel

[0080] Same as Example 4, except that the composition of the gel is:

[0081] The pH is adjusted to 5.5 by triethanolamine, and the following components are contained in parts by weight: 45 parts of the Chinese medicine extract obtained in Example 1, 25 parts of the gel matrix, 0.15 parts of chlorhexidine acetate, 1 part of propolis, and water is added to make up to 100 parts.

[0082] Example 8 Preparation of gel

[0083] Same as Example 4, except that the composition of the gel is:

[0084] The pH is adjusted to 5.5 by triethanolamine, and the mixture contains the following components in parts by weight: 45 parts of the Chinese herbal extract obtained in Example 1, 25 parts of a gel matrix, 0.15 parts of chlorhexidine acetate, 2 parts of propolis, and water to make up to 100 parts.

[0085] Example 9 Preparation of gel

[0086] Same as Example 4, except that the composition of the gel is:

[0087] The pH is adjusted to 5.5 by triethanolamine, and the mixture contains the following components in parts by weight: 45 parts of the Chinese herbal extract obtained in Example 1, 25 parts of a gel matrix, 0.15 parts of chlorhexidine acetate, 0.2 parts of vitamin E, and water to make up to 100 parts.

[0088] Example 10 Preparation of gel

[0089] Same as Example 4, except that the composition of the gel is:

[0090] The pH is adjusted to 5.5 by triethanolamine, and the mixture contains the following components in parts by weight: 45 parts of the Chinese herbal extract obtained in Example 1, 25 parts of a gel matrix, 0.15 parts of chlorhexidine acetate, 0.5 parts of vitamin E, and water to make up to 100 parts.

[0091] Example 11 Preparation of gel

[0092] Same as Example 4, except that the composition of the gel is:

[0093] The pH value was adjusted to 5.5 by triethanolamine, and the mixture contained the following components in parts by weight: 45 parts of the Chinese herbal extract obtained in Example 1, 25 parts of the gel matrix, and 100 parts of water.

[0094] Comparative Example 1 Preparation of Chinese herbal medicine extract

[0095] Same as Example 1, except that Sophora flavescens is replaced by Codonopsis pilosula.

[0096] Comparative Example 2 Preparation of Chinese herbal medicine extract

[0097] Same as Example 1, except that Phellodendron chinense is replaced by Scutellaria baicalensis.

[0098] Comparative Example 3 Preparation of Chinese herbal medicine extract

[0099] Same as Example 1, except that honeysuckle is replaced by dandelion.

[0100] Comparative Example 4 Preparation of Gel

[0101] Same as Example 4, except that the Chinese herbal extract obtained in Example 1 is replaced by the Chinese herbal extract obtained in Comparative Example 1.

[0102] Comparative Example 5 Preparation of Gel

[0103] The same as Example 4, except that the Chinese herbal extract obtained in Example 1 is replaced by the Chinese herbal extract obtained in Comparative Example 2.

[0104] Comparative Example 6 Preparation of Gel

[0105] The same as Example 4, except that the Chinese herbal extract obtained in Example 1 is replaced by the Chinese herbal extract obtained in Comparative Example 3.

[0106] Test Example 1 Antibacterial Test

[0107] According to the method in Appendix E of GB 15979-2024, the inhibitory effects of the Chinese medicine extracts obtained in Examples 1 to 3 and Comparative Examples 1 to 3 and the gels obtained in Examples 4 to 11 and Comparative Examples 4 to 6 on Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC25923), and Candida albicans (ATCC 10231) were tested. The results are shown in Tables 1 and 2.

[0108] Table 1 Antibacterial test results of Chinese herbal medicine extracts

[0109] Escherichia coli inhibition rate / % Staphylococcus aureus inhibition rate / % Candida albicans inhibition rate / % Example 1 92.23 93.24 91.05 Example 2 90.82 91.78 90.72 Example 3 92.01 92.65 90.18 Comparative Example 1 81.35 78.96 75.48 Comparative Example 2 82.79 85.04 79.73 Comparative Example 3 86.42 85.86 84.35

[0110] Table 2 Antibacterial test results of gel

[0111]

[0112]

[0113]

[0114] It can be seen that the antibacterial performance of the Chinese medicine extracts obtained in Examples 1 to 3 is significantly better than that of Comparative Examples 1 to 3, and the antibacterial performance of the gels obtained in Examples 4 to 11 is also significantly better than that of Comparative Examples 4 to 6, and the antibacterial rates of the Chinese medicine extracts obtained in Examples 1 to 3 and the gels obtained in Examples 4 to 11 are all above 90%. This shows that the Chinese medicine composition obtained by the present invention adopts specific Chinese medicine ingredients for compounding, and after water extraction, compared with the products obtained by water extraction of other Chinese medicine ingredients, it plays a more excellent role in inhibiting pathogenic microorganisms such as Escherichia coli, Staphylococcus aureus, and Candida albicans, and is more suitable for preparing products that inhibit pathogenic microorganisms and drugs for treating vaginitis. In addition, making the Chinese medicine extract of the present invention into a gel will not affect its performance in inhibiting pathogenic microorganisms.

[0115] Test Example 2 Trichomonas inhibition test

[0116] Take 0.5 mL of the Chinese medicine extracts obtained in Examples 1 to 3 and Comparative Examples 1 to 3, and the gels obtained in Examples 4 to 11 and Comparative Examples 4 to 6, and add them to 9.5 mL of the Trichomonas solution (with a Trichomonas content of 10 5 / mL), oscillate evenly, and after culturing at 37°C for 24 hours, count the cells with a cell counter under a microscope and calculate the trichomonas inhibition rate. Each test was repeated 3 times, and the results were averaged. The results are shown in Tables 3 and 4.

[0117] Table 3 Trichomonas inhibition test results of Chinese herbal medicine extracts

[0118] Trichomonas inhibition rate / % Example 1 99.07 Example 2 98.95 Example 3 98.26 Comparative Example 1 72.92 Comparative Example 2 79.21 Comparative Example 3 91.34

[0119] Table 4 Trichomonas inhibition test results of gel

[0120]

[0121]

[0122] It can be seen that the trichomonas inhibition performance of the Chinese medicine extracts obtained in Examples 1 to 3 is significantly better than that of Comparative Examples 1 to 3, and the trichomonas inhibition performance of the gels obtained in Examples 4 to 11 is also significantly better than that of Comparative Examples 4 to 6, and the trichomonas inhibition rates of the Chinese medicine extracts obtained in Examples 1 to 3 and the gels obtained in Examples 4 to 11 are all above 97%. It shows that the Chinese medicine composition obtained by the present invention adopts specific Chinese medicine ingredients for compounding, and after water extraction, it plays a more excellent role in inhibiting pathogenic microorganisms such as trichomonas compared with the products obtained by water extraction of other Chinese medicine ingredients, and is more suitable for preparing products that inhibit pathogenic microorganisms and drugs for treating vaginitis. In addition, making the Chinese medicine extract of the present invention into a gel will not affect its performance in inhibiting pathogenic microorganisms.

[0123] Test Example 3 Vaginal mucosal repair test

[0124] Vaginal mucosal tissue samples collected from the vagina of female New Zealand white rabbits were cut into 4 mm wide strips, and 40 times the volume of 0.25% trypsin digestion solution with 1 mg / mL collagenase was added for digestion. After digestion at 37°C for 45 minutes, mucosal epithelial cells were isolated. Mucosal epithelial cells were inoculated in DMEM / F12 culture medium containing 10% (v / v) fetal bovine serum, cultured at 37°C and 5% CO2, and the culture medium was changed every 48 hours. After 6 days, mucosal epithelial cells with good growth status were obtained.

[0125] The gel obtained in Examples 4 to 11 was added to a DMEM / F12 culture medium containing 10% (v / v) fetal bovine serum at a ratio of 0.2 g / mL, extracted at 37°C for 48 hours, and then centrifuged at 4000 rpm for 10 minutes. The supernatant obtained by centrifugation was test solutions 1 to 8 (test solutions 1 to 8 were the test solutions prepared from the gel obtained in Examples 4 to 11, respectively).

[0126] Add 100 μL of 10% 4The mucosal epithelial cell suspension of 100 μL / mL was cultured at 37°C and 5% CO2 for 24 hours, the original culture medium was discarded, 100 μL of DMEM / F12 medium containing 10% (v / v) fetal bovine serum (as a blank control group) and test solutions 1 to 8 (as a test group) were added respectively, and the culture was continued at 37°C and 5% CO2 for 72 hours, and then 5 g / L of MTT solution was added to each well, and the liquid in the well was discarded after continuing to culture for 4 hours, and 150 μL of DMSO was added. The absorbance OD of each well at 570 nm was detected by a multifunctional microplate reader, and finally the relative proliferation rate (RGR) of test solutions 1 to 8 was calculated according to "Relative proliferation rate RGR (%) = test group OD / blank control group OD × 100%". Three parallel wells were set for each group, and the results were averaged. The results are shown in Table 5.

[0127] Table 5 Vaginal mucosal repair test results

[0128] Relative proliferation rate / % Test solution 1 (Example 4) 132.0 Test solution 2 (Example 5) 128.4 Test solution 3 (Example 6) 131.2 Test solution 4 (Example 7) 181.6 Test solution 5 (Example 8) 186.2 Test solution 6 (Example 9) 188.7 Test solution 7 (Example 10) 190.8 Test solution 8 (Example 11) 132.2

[0129] It can be seen that in the vaginal mucosal repair test, the relative proliferation rate of mucosal epithelial cells of the gels obtained in Examples 4 to 11 was higher than 130%, indicating that the gel of the present invention can not only effectively inhibit pathogenic microorganisms, but also effectively repair the vaginal mucosa, and is very suitable for preparing vaginal mucosal repair products.

[0130] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A Chinese medicine extract, characterized in that The Chinese medicine composition is obtained by water extraction; the Chinese medicine composition comprises the following components in parts by weight: 15-20 parts of Sophora flavescens, 25-30 parts of Phellodendron amurense, 15-20 parts of Cnidium monnieri, 15-20 parts of Kochia scoparia, 8-12 parts of Stemona, 8-12 parts of Lithospermum officinale, 8-12 parts of Atractylodes macrocephala, 12-20 parts of Leonurus japonicus, 12-20 parts of Honeysuckle and 8-12 parts of Saussurea involucrata.

2. The Chinese herbal extract according to claim 1, characterized in that The water extraction is carried out at 95-100° C. for 1.5-2 hours.

3. A gel, characterized in that: The pH value is 4.5-6, and the gel comprises the following components in parts by weight: 40-50 parts of the Chinese herbal extract according to claim 1, 20-30 parts of gel matrix, and 100 parts of water.

4. The gel according to claim 3, characterized in that The gel matrix comprises the following components in parts by weight: 1.8-2.2 parts of carbomer, 0.5-1 part of hyaluronic acid, and water to make up to 100 parts.

5. The gel according to claim 3, characterized in that: The pH value is 4.5-6, and the gel comprises the following components in parts by weight: 40-50 parts of the Chinese herbal extract according to claim 1, 20-30 parts of a gel matrix, 0.02-0.3 parts of chlorhexidine acetate, and 100 parts of water.

6. The gel according to claim 3, characterized in that: The pH value is 4.5-6, and the gel comprises the following components in parts by weight: 40-50 parts of the Chinese herbal extract according to claim 1, 20-30 parts of gel matrix, 0.02-0.3 parts of chlorhexidine acetate, 1-2 parts of propolis, and 100 parts of water.

7. The gel according to claim 3, characterized in that The pH value is 4.5-6, and the gel comprises the following components in parts by weight: 40-50 parts of the Chinese herbal extract according to claim 1, 20-30 parts of gel matrix, 0.02-0.3 parts of chlorhexidine acetate, 0.2-0.5 parts of vitamin E, and 100 parts of water.

8. Use of the Chinese herbal extract according to any one of claims 1 to 2 or the gel according to any one of claims 3 to 7 in the preparation of a product for inhibiting pathogenic microorganisms, characterized in that: The pathogenic microorganism is one or more of Escherichia coli, Staphylococcus aureus, Candida albicans and Trichomonas.

9. Use of the Chinese herbal extract according to any one of claims 1 to 2 or the gel according to any one of claims 3 to 7 in the preparation of a medicament for treating vaginitis, characterized in that: The vaginitis is one or more of Escherichia coli vaginitis, Staphylococcus aureus vaginitis, Candida albicans vaginitis, and Trichomonas vaginitis.

10. Use of the gel according to any one of claims 3 to 7 in the preparation of vaginal mucosal repair products.

Images