Longitudinal modulus-shear modulus synchronous detection device for biological tissue

Abstract

The invention discloses a longitudinal modulus-shear modulus synchronous detection device for biological tissue, and relates to the technical field of elasticity imaging of biological tissue to be detected, in the device, a three-dimensional displacement table is arranged on a double-angle input and output light path of a double-angle Brillouin scattering signal generating and receiving assembly; the optical fiber coupler is arranged on a coupling output optical path of the double-angle Brillouin scattering signal generating and receiving assembly; the measuring assembly is connected with the optical fiber coupler through an optical fiber; the three-dimensional displacement table moves to enable a double-angle focus point of the double-angle Brillouin scattering signal generating and receiving assembly to coincide with a target detection point; the double-angle Brillouin scattering signal generating and receiving assembly excites and receives a Brillouin scattering signal at a target detection point; according to the invention, the dual-angle Brillouin scattering signal generating and receiving assembly is arranged, so that synchronous detection of a longitudinal modulus spectrum and a shear modulus spectrum can be completed.

Description

Technical Field

[0001] The present application relates to the technical field of elastic imaging of biological tissue to be tested, and in particular to a synchronous detection device for longitudinal modulus-shear modulus of biological tissue. Background Art

[0002] The longitudinal modulus of the biological tissue to be tested is not exactly the same as Young's modulus. The longitudinal modulus is also related to the shear modulus and bulk modulus. The test sample biological tissue to be tested is different from the ordinary tensile modulus: as a highly hydrated biological tissue to be tested, due to the high incompressibility of water, the longitudinal modulus of Brillouin detection is closer to the bulk modulus. A detection device is required to simultaneously obtain the shear modulus and longitudinal modulus of the biological tissue to be tested, and obtain the spectra of the two moduli through a high-resolution spectrometer to determine whether the shear modulus and longitudinal modulus are related. Summary of the invention

[0003] The purpose of the present application is to provide a synchronous detection device for the longitudinal modulus and shear modulus of biological tissue, which can complete the synchronous detection of the longitudinal modulus spectrum and the shear modulus spectrum.

[0004] To achieve the above objectives, this application provides the following solutions:

[0005] The present application provides a synchronous detection device for the longitudinal modulus and shear modulus of biological tissue, comprising: a three-dimensional displacement stage, a dual-angle Brillouin scattering signal generating and receiving component, an optical fiber coupler and a measuring component;

[0006] The three-dimensional displacement stage is arranged on the dual-angle input and output optical path of the dual-angle Brillouin scattering signal generating and receiving component; the optical fiber coupler is arranged on the coupled output optical path of the dual-angle Brillouin scattering signal generating and receiving component; the measuring component is connected to the optical fiber coupler through an optical fiber;

[0007] The three-dimensional displacement stage is used to place the biological tissue to be tested; the three-dimensional displacement stage is used to drive the biological tissue to be tested to move so that the dual-angle focusing point of the dual-angle Brillouin scattering signal receiving component coincides with the target detection point;

[0008] The dual-angle Brillouin scattering signal generating and receiving component is used to emit laser light from two angles to focus on a target detection point on the biological tissue to be detected; the dual-angle Brillouin scattering signal generating and receiving component is also used to receive a Brillouin scattering signal at the target detection point; the Brillouin scattering signal includes backscattered light, first side scattered light and second side scattered light; the Brillouin scattering signal is generated after the biological tissue to be detected at the target detection point is irradiated by laser light at two angles;

[0009] The optical fiber coupler is used to couple the backscattered light, the first side scattered light and the second side scattered light at the target detection point into a coupled light beam, and input the coupled light beam into the measurement component;

[0010] The measuring component is used to separate and collect Brillouin scattering signals based on the coupled light beam; the Brillouin scattering signals are used to express the longitudinal modulus and shear modulus at the target detection point.

[0011] Optionally, the dual-angle Brillouin scattering signal generating and receiving component includes: a laser emitting component, a polarization beam splitter, a first angle input and output optical path component, and a second angle input and output optical path component;

[0012] The polarization beam splitter is arranged on the output light path of the laser emitting component;

[0013] The first angle input and output optical path component is arranged on the first angle input and output optical path of the polarization beam splitter;

[0014] The second angle input and output optical path component is arranged on the second angle input and output optical path of the polarization beam splitter;

[0015] The laser emitting component is used to emit laser;

[0016] The polarization beam splitter is used to split the laser into a reflected light beam and a transmitted light beam;

[0017] The first angle input and output optical path component is used to focus the reflected light beam to the target detection point; the first angle input and output optical path is also used to obtain backscattered light and first side scattered light;

[0018] The second angle input and output optical path component is used to focus the transmitted light beam to the target detection point; the second angle input and output optical path is also used to obtain the second side scattered light;

[0019] The reflected output light path and the transmitted output light path of the polarization beam splitter are collinear; the reflected output light path and the transmitted output light path of the polarization beam splitter serve as coupled output light paths of the dual-angle Brillouin scattering signal generating and receiving component;

[0020] The reflected output light path of the polarization beam splitter is the reflected output light path of the second side scattered light through the polarization beam splitter; the transmitted output light path of the polarization beam splitter is the transmitted output light path of the backscattered light and the first side scattered light through the polarization beam splitter.

[0021] Optionally, the laser emission assembly includes: a laser, a first reflector and a half-wave plate;

[0022] The first reflector is arranged on the output optical path of the laser;

[0023] The half wave plate is arranged on the output light path of the first reflector;

[0024] The polarization beam splitter is arranged on the output light path of the half wave plate.

[0025] Optionally, the laser is a 532nm laser or a 780nm laser.

[0026] Optionally, the first angle input and output optical path component includes: a quarter wave plate, a second reflector and a first focusing objective lens;

[0027] The quarter wave plate is arranged on the first angle input and output optical path of the polarization beam splitter;

[0028] The second reflector is arranged on the output light path of the quarter wave plate;

[0029] The first focusing objective lens is arranged on the output light path of the second reflecting mirror;

[0030] The three-dimensional translation stage is arranged on the output light path of the first focusing objective lens.

[0031] Optionally, the second angle input and output optical path component includes: a third reflector and a second focusing objective lens;

[0032] The third reflector is arranged on the second angle input and output optical path of the polarization beam splitter;

[0033] The second focusing objective lens is arranged on the output light path of the third reflecting mirror;

[0034] The three-dimensional translation stage is arranged on the output light path of the second focusing objective lens.

[0035] Optionally, the measuring component includes: a spectrometer and a signal collector;

[0036] The spectrometer is connected to the optical fiber coupler via an optical fiber; the spectrometer is used to separate the coupled light beam into Brillouin scattering signals;

[0037] The signal collector is arranged on the output optical path of the spectrometer; the signal collector is used to collect Brillouin scattering signals.

[0038] Optionally, the spectrometer comprises: a collimator, a first cylindrical lens, a first virtual imaging phase array, a fourth reflector, a second cylindrical lens, a first lens, a fifth reflector, a second virtual imaging phase array and a second lens;

[0039] The collimator is connected to the fiber coupler via an optical fiber;

[0040] The first cylindrical lens is arranged on the output light path of the collimator;

[0041] The first virtual imaging phase array is arranged on the output light path of the first cylindrical lens;

[0042] The fourth reflector is arranged on the output optical path of the first virtual imaging phase array;

[0043] The second cylindrical lens is arranged on the output light path of the fourth reflector;

[0044] The first lens is arranged on the output light path of the second cylindrical lens;

[0045] The fifth reflector is arranged on the output light path of the first lens;

[0046] The second virtual imaging phase array is arranged on the output optical path of the fifth reflector;

[0047] The second lens is arranged on the output optical path of the second virtual imaging phase array;

[0048] The signal collector is arranged on the output optical path of the second lens.

[0049] Optionally, the signal collector is an electron multiplying CCD.

[0050] Optionally, the three-dimensional displacement platform is provided with a first bracket and a second bracket;

[0051] The first bracket is rotatably connected to the three-dimensional displacement stage; the first bracket is used to place the first focusing objective lens;

[0052] The second bracket is rotatably connected to the three-dimensional translation stage; the second bracket is used to place the second focusing objective lens;

[0053] Rotating the first bracket and the second bracket can adjust the angle of the Brillouin scattering signal.

[0054] According to the specific embodiments provided in this application, this application discloses the following technical effects:

[0055] The present application provides a device for synchronous detection of the longitudinal modulus and shear modulus of biological tissues. By measuring the dual-angle Brillouin scattering frequency shift of the biological tissue to be tested, the longitudinal wave velocity and the shear wave velocity are obtained, thereby realizing synchronous detection of the longitudinal modulus and shear modulus of the biological tissue to be tested. The device has a simple structure and can realize synchronous measurement of the longitudinal modulus and shear modulus of the tissue. It is of great significance in the mechanical characteristics of the biological tissue to be tested, and can provide a scientific basis for the early diagnosis, treatment and prevention of diseases of the biological tissue to be tested, and provide a technical path for the development of tissue biomechanical property detection equipment and lay a key technical foundation. BRIEF DESCRIPTION OF THE DRAWINGS

[0056] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0057] Figure 1 A schematic diagram of a device for synchronously detecting the longitudinal modulus and shear modulus of biological tissue in one embodiment of the present application;

[0058] Figure 2 This is a schematic structural diagram of a device for synchronously detecting the longitudinal modulus and shear modulus of biological tissue in one embodiment of the present application;

[0059] Figure 3 It is a schematic diagram of a first angle between a three-dimensional translation stage, a first focusing lens, and a second focusing lens in one embodiment of the present application;

[0060] Figure 4 Schematic diagram of the second angle between the three-dimensional translation stage, the first focusing lens and the second focusing lens in one embodiment of the present application.

[0061] Figure numerals: laser 1; first reflector 2; half wave plate 3; polarization beam splitter 4; quarter wave plate 5; second reflector 6; first focusing objective lens 7; third reflector 8; second focusing objective lens 9; scanning device 10; fiber coupler 11; optical fiber 12; spectrometer 13; collimator 14; first cylindrical lens 15; first virtual imaging phase array 16; fourth reflector 17; second cylindrical lens 18; first lens 19; fifth reflector 20; second virtual imaging phase array 21; second lens 22; signal collector 23; three-dimensional translation stage 25; biological tissue to be measured 24. DETAILED DESCRIPTION

[0062] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0063] In order to make the above-mentioned objects, features and advantages of the present application more obvious and easy to understand, the present application is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0064] In an exemplary embodiment, Figures 1 to 4As shown, a device for synchronously detecting the longitudinal modulus and shear modulus of biological tissue is provided, comprising: a three-dimensional displacement stage 25, a dual-angle Brillouin scattering signal generating and receiving component, a fiber coupler 11 and a measuring component.

[0065] The three-dimensional displacement stage 25 is arranged on the dual-angle input and output optical path of the dual-angle Brillouin scattering signal generating and receiving component. The optical fiber coupler 11 is arranged on the coupled output optical path of the dual-angle Brillouin scattering signal generating and receiving component. The measuring component is connected to the optical fiber coupler 11 through the optical fiber 12. The three-dimensional displacement stage 25 is used to place the biological tissue 24 to be measured. The three-dimensional displacement stage 25 is used to drive the biological tissue 24 to be measured to move so that the dual-angle focusing point of the dual-angle Brillouin scattering signal generating and receiving component coincides with the target detection point. The dual-angle Brillouin scattering signal generating and receiving component is used to emit lasers from two angles to focus on the target detection point on the biological tissue 24 to be measured. The dual-angle Brillouin scattering signal generating and receiving component is also used to receive the Brillouin scattering signal at the target detection point. The Brillouin scattering signal includes backscattered light, first side scattered light and second side scattered light. The Brillouin scattering signal is generated after the biological tissue 24 to be measured at the target detection point is irradiated by two-angle lasers. The optical fiber coupler 11 is used to couple the backscattered light, the first side scattered light and the second side scattered light at the target detection point into a coupled light beam, and input the coupled light beam into the measurement component. The measurement component is used to separate and collect the Brillouin scattering signal based on the coupled light beam. The Brillouin scattering signal is used to express the longitudinal modulus and shear modulus at the target detection point.

[0066] The dual-angle Brillouin scattering signal generating and receiving component includes: a laser emitting component, a polarization beam splitter 4, a first angle input and output optical path component and a second angle input and output optical path component. The polarization beam splitter 4 is arranged on the output optical path of the laser emitting component. The first angle input and output optical path component is arranged on the first angle input and output optical path of the polarization beam splitter 4. The second angle input and output optical path component is arranged on the second angle input and output optical path of the polarization beam splitter 4. The laser emitting component is used to emit laser. The polarization beam splitter 4 is used to divide the laser into a reflected light beam and a transmitted light beam. The first angle input and output optical path component is used to focus the reflected light beam to the target detection point. The first angle input and output optical path is also used to obtain backscattered light and first side scattered light. The second angle input and output optical path component is used to focus the transmitted light beam to the target detection point. The second angle input and output optical path is also used to obtain the second side scattered light. The reflected output optical path and the transmitted output optical path of the polarization beam splitter 4 are collinear. The reflected output optical path and the transmitted output optical path of the polarization beam splitter 4 serve as the coupled output optical path of the dual-angle Brillouin scattering signal generating and receiving component. The reflected output light path of the polarization beam splitter 4 is the reflected output light path of the second side scattered light through the polarization beam splitter 4. The transmitted output light path of the polarization beam splitter 4 is the transmitted output light path of the backscattered light and the first side scattered light through the polarization beam splitter 4.

[0067] The laser emission assembly includes: a laser 1, a first reflector 2 and a half-wave plate 3. The first reflector 2 is arranged on the output light path of the laser 1. The half-wave plate 3 is arranged on the output light path of the first reflector 2. The polarization beam splitter 4 is arranged on the output light path of the half-wave plate 3. The laser 1 is a 532nm laser or a 780nm laser.

[0068] The first angle input and output optical path component includes: a quarter wave plate 5, a second reflector 6 and a first focusing lens 7. The quarter wave plate 5 is arranged on the first angle input and output optical path of the polarization beam splitter 4. The second reflector 6 is arranged on the output optical path of the quarter wave plate 5. The first focusing lens 7 is arranged on the output optical path of the second reflector 6. The three-dimensional displacement stage 25 is arranged on the output optical path of the first focusing lens 7.

[0069] The second angle input and output optical path assembly includes: a third reflector 8 and a second focusing lens 9. The third reflector 8 is arranged on the second angle input and output optical path of the polarization beam splitter 4. The second focusing lens 9 is arranged on the output optical path of the third reflector 8. The three-dimensional displacement stage 25 is arranged on the output optical path of the second focusing lens 9.

[0070] The measuring assembly includes: a spectrometer 13 and a signal collector 23. The spectrometer 13 is connected to the optical fiber coupler 11 through an optical fiber 12. The spectrometer 13 is used to separate the coupled light beam into Brillouin scattering signals. The signal collector 23 is arranged on the output optical path of the spectrometer 13. The signal collector 23 is used to collect Brillouin scattering signals. The signal collector 23 is an electron multiplying CCD.

[0071] The spectrometer 13 includes: a collimator 14, a first cylindrical lens 15, a first virtual imaging phase array 16, a fourth reflector 17, a second cylindrical lens 18, a first lens 19, a fifth reflector 20, a second virtual imaging phase array 21 and a second lens 22. The collimator 14 is connected to the fiber coupler 11 through the optical fiber 12. The first cylindrical lens 15 is arranged on the output optical path of the collimator 14. The first virtual imaging phase array 16 is arranged on the output optical path of the first cylindrical lens 15. The fourth reflector 17 is arranged on the output optical path of the first virtual imaging phase array 16. The second cylindrical lens 18 is arranged on the output optical path of the fourth reflector 17. The first lens 19 is arranged on the output optical path of the second cylindrical lens 18. The fifth reflector 20 is arranged on the output optical path of the first lens 19. The second virtual imaging phase array 21 is arranged on the output optical path of the fifth reflector 20. The second lens 22 is arranged on the output optical path of the second virtual imaging phase array 21. The signal collector 23 is arranged on the output optical path of the second lens 22.

[0072] A first bracket and a second bracket are provided on the three-dimensional displacement stage 25. The first bracket is rotatably connected to the three-dimensional displacement stage 25. The first bracket is used to place the first focusing lens 7. The second bracket is rotatably connected to the three-dimensional displacement stage 25. The second bracket is used to place the second focusing lens 9. Rotating the first bracket and the second bracket can adjust the angle of the Brillouin scattering signal.

[0073] As another embodiment, Figures 1 to 4 , a synchronous detection device for the longitudinal modulus and shear modulus of biological tissues, comprising: a dual-angle Brillouin scattering signal generating device, a dual-angle signal receiving device, a spectrometer 13 capable of simultaneously distinguishing two kinds of angular dispersion, a scanning device 10 and a signal collector 23; the scanning device 10 can realize multi-dimensional scanning; the dual-angle Brillouin scattering signal generating device and the dual-angle signal receiving device share some units; the dual-angle signal receiving device can be used to simultaneously receive Brillouin scattering signals of two different angles of a biological tissue 24 to be measured and collect them into the same signal collector, and perform three-dimensional synchronous measurement of the longitudinal modulus and shear modulus of the biological tissue 24 to be measured through a three-dimensional displacement stage 25.

[0074] The dual-angle Brillouin signal generating device comprises: a laser 1, a first reflector 2, a half-wave plate 3, a polarization beam splitter 4, a quarter-wave plate 5, a second reflector 6, a first focusing objective lens 7, a third reflector 8 and a second focusing objective lens 9, a scanning device 10, a fiber coupler 11 and an optical fiber 12. The dual-angle Brillouin signal generating device is used to collect all Brillouin scattering spectrum signals and transmit them to a spectrometer 13; the scanning device 10 comprises: a biological tissue to be measured 24 and a three-dimensional displacement stage 25. The scanning device 10 is used to move the biological tissue to be measured so as to measure the Brillouin scattering frequency shift of the tissue in a three-dimensional direction.

[0075] The Brillouin signal is emitted by laser 1 and directed to the first reflector 2, which reflects the laser to the half wave plate 3. The laser is then split into a quarter wave plate 5 and a third reflector 8 after passing through a polarization beam splitter 4; the light passing through the quarter wave plate 5 is reflected by the second reflector 6 into the first focusing objective lens 7 and irradiated onto the biological tissue 24 to be tested to generate Brillouin scattering; the light passing through the third reflector 8 is reflected into the second focusing objective lens 9 and irradiated onto the biological tissue 24 to be tested to generate Brillouin scattering. Among them, the laser 1 is a single-mode narrow linewidth laser, and its wavelength can be 532nm or 780nm; but the wavelength of the laser 1 is not limited to 532nm and 780nm. The polarization beam splitter 4 is a PBS polarization beam splitter.

[0076] The first focusing lens 7 focuses the laser onto the biological tissue 24 to be tested and generates a Brillouin scattering signal through photoacoustic effect, wherein the backward Brillouin scattered light returns to the first focusing lens 7 along the original path, is reflected by the second reflector 6 to the quarter wave plate 5, enters the polarization beam splitter 4 through the quarter wave plate 5 and is transmitted to the fiber coupler 11; the first focusing lens 7 focuses the laser onto the biological tissue 25 to be tested and generates a Brillouin scattering signal through photoacoustic effect, wherein the side Brillouin scattered light enters the second focusing lens 9 along the direction of the second focusing lens 9 and is reflected by the third reflector 8 into the polarization beam splitter 4, and part of the side scattered light is then reflected by the polarization beam splitter 4 into the fiber coupler 11. In addition, the second focusing lens 9 focuses the laser onto the biological tissue 24 to be tested and generates a Brillouin scattering signal through photoacoustic effect, wherein the side Brillouin scattered light enters the first focusing lens 7 along the direction of the first focusing lens 7 and is reflected by the second reflector 6 into the quarter wave plate 5, and part of the side scattered light passing through the quarter wave plate 5 is transmitted by the polarization beam splitter 4 into the fiber coupler 11.

[0077] After the laser 1 outputs the laser, it passes through the half wave plate 3 to change the polarization state of the laser. When the laser passes through the polarization beam splitter 4, part of the light is transmitted and part of the light is reflected. When the first focusing lens 7 receives the backward 180° scattered light, the other second focusing lens 9 will simultaneously receive the non-180° Brillouin scattered light. Figure 3 and Figure 4 By changing the angle between the first focusing lens 7 and the second focusing lens 9, Brillouin scattered light of multiple angles including 180° and 90° can be obtained.

[0078] The dual-angle signal receiving device includes: a first focusing lens 7, a second focusing lens 9, a quarter-wave plate 5, a second reflector 6, a third reflector 8, a polarization beam splitter 4, a fiber coupler 11, and an optical fiber 12. The dual-angle signal receiving device is used to collect dual-angle Brillouin scattering spectrum signals and transmit the dual-angle Brillouin scattering spectrum signals to the spectrometer 13; the signal collector 23 includes an electron multiplying CCD, and the signal collector 23 is used to collect Brillouin signals to generate sample shear elastic modulus information. The electron multiplying CCD can be replaced by other photoelectric receivers. During each measurement, the lasers emitted by the first focusing lens 7 and the second focusing lens 9 are simultaneously focused on the same point of the biological tissue 24 to be measured for signal collection, and then scanned and measured point by point.

[0079] The technical features of the above embodiments may be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0080] Specific examples are used herein to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only used to help understand the device and its core idea of ​​the present application. At the same time, for those skilled in the art, according to the idea of ​​the present application, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present application.

Claims

1. A synchronous detection device for longitudinal modulus and shear modulus of biological tissue, characterized in that: include: Three-dimensional translation stage, dual-angle Brillouin scattering signal generation and receiving components, optical fiber coupler and measurement components; The three-dimensional displacement stage is arranged on the dual-angle input and output optical path of the dual-angle Brillouin scattering signal generating and receiving component; the optical fiber coupler is arranged on the coupled output optical path of the dual-angle Brillouin scattering signal generating and receiving component; the measuring component is connected to the optical fiber coupler through an optical fiber; The three-dimensional displacement stage is used to place the biological tissue to be tested; the three-dimensional displacement stage is used to drive the biological tissue to be tested to move so that the dual-angle focusing point of the dual-angle Brillouin scattering signal receiving component coincides with the target detection point; The dual-angle Brillouin scattering signal generating and receiving component is used to emit laser light from two angles to focus on a target detection point on the biological tissue to be detected; the dual-angle Brillouin scattering signal generating and receiving component is also used to receive a Brillouin scattering signal at the target detection point; the Brillouin scattering signal includes backscattered light, first side scattered light and second side scattered light; the Brillouin scattering signal is generated after the biological tissue to be detected at the target detection point is irradiated by laser light at two angles; The optical fiber coupler is used to couple the backscattered light, the first side scattered light and the second side scattered light at the target detection point into a coupled light beam, and input the coupled light beam into the measurement component; The measuring component is used to separate and collect Brillouin scattering signals based on the coupled light beam; the Brillouin scattering signals are used to express the longitudinal modulus and shear modulus at the target detection point.

2. The device for synchronously detecting longitudinal modulus and shear modulus of biological tissue according to claim 1, characterized in that: The dual-angle Brillouin scattering signal generating and receiving component comprises: a laser emitting component, a polarization beam splitter, a first angle input and output optical path component and a second angle input and output optical path component; The polarization beam splitter is arranged on the output light path of the laser emitting component; The first angle input and output optical path component is arranged on the first angle input and output optical path of the polarization beam splitter; The second angle input and output optical path component is arranged on the second angle input and output optical path of the polarization beam splitter; The laser emitting component is used to emit laser; The polarization beam splitter is used to split the laser into a reflected light beam and a transmitted light beam; The first angle input and output optical path component is used to focus the reflected light beam to the target detection point; the first angle input and output optical path is also used to obtain backscattered light and first side scattered light; The second angle input and output optical path component is used to focus the transmitted light beam to the target detection point; the second angle input and output optical path is also used to obtain the second side scattered light; The reflected output light path and the transmitted output light path of the polarization beam splitter are collinear; the reflected output light path and the transmitted output light path of the polarization beam splitter serve as coupled output light paths of the dual-angle Brillouin scattering signal generating and receiving component; The reflected output light path of the polarization beam splitter is the reflected output light path of the second side scattered light through the polarization beam splitter; the transmitted output light path of the polarization beam splitter is the transmitted output light path of the backscattered light and the first side scattered light through the polarization beam splitter.

3. The synchronous detection device for longitudinal modulus and shear modulus of biological tissue according to claim 2, characterized in that: The laser emission assembly comprises: a laser, a first reflector and a half-wave plate; The first reflector is arranged on the output optical path of the laser; The half wave plate is arranged on the output light path of the first reflector; The polarization beam splitter is arranged on the output light path of the half wave plate.

4. The device for synchronously detecting longitudinal modulus and shear modulus of biological tissue according to claim 3, characterized in that: The laser is a 532nm laser or a 780nm laser.

5. The device for synchronously detecting longitudinal modulus and shear modulus of biological tissue according to claim 3, characterized in that: The first angle input and output optical path components include: a quarter wave plate, a second reflector and a first focusing objective lens; The quarter wave plate is arranged on the first angle input and output optical path of the polarization beam splitter; The second reflector is arranged on the output light path of the quarter wave plate; The first focusing objective lens is arranged on the output light path of the second reflecting mirror; The three-dimensional translation stage is arranged on the output light path of the first focusing objective lens.

6. The device for synchronously detecting longitudinal modulus and shear modulus of biological tissue according to claim 5, characterized in that: The second angle input and output optical path component includes: a third reflector and a second focusing objective lens; The third reflector is arranged on the second angle input and output optical path of the polarization beam splitter; The second focusing objective lens is arranged on the output light path of the third reflecting mirror; The three-dimensional translation stage is arranged on the output light path of the second focusing objective lens.

7. The device for synchronously detecting longitudinal modulus and shear modulus of biological tissue according to claim 3, characterized in that: The measuring component comprises: a spectrometer and a signal collector; The spectrometer is connected to the optical fiber coupler via an optical fiber; the spectrometer is used to separate the coupled light beam into Brillouin scattering signals; The signal collector is arranged on the output optical path of the spectrometer; the signal collector is used to collect Brillouin scattering signals.

8. The device for synchronously detecting longitudinal modulus and shear modulus of biological tissue according to claim 7, characterized in that: The spectrometer comprises: a collimator, a first cylindrical lens, a first virtual imaging phase array, a fourth reflector, a second cylindrical lens, a first lens, a fifth reflector, a second virtual imaging phase array and a second lens; The collimator is connected to the fiber coupler via an optical fiber; The first cylindrical lens is arranged on the output light path of the collimator; The first virtual imaging phase array is arranged on the output light path of the first cylindrical lens; The fourth reflector is arranged on the output optical path of the first virtual imaging phase array; The second cylindrical lens is arranged on the output light path of the fourth reflector; The first lens is arranged on the output light path of the second cylindrical lens; The fifth reflector is arranged on the output light path of the first lens; The second virtual imaging phase array is arranged on the output optical path of the fifth reflector; The second lens is arranged on the output optical path of the second virtual imaging phase array; The signal collector is arranged on the output optical path of the second lens.

9. The device for synchronously detecting longitudinal modulus and shear modulus of biological tissue according to claim 7, characterized in that: The signal collector is an electron multiplying CCD.

10. The device for synchronously detecting longitudinal modulus and shear modulus of biological tissue according to claim 6, characterized in that: The three-dimensional displacement platform is provided with a first bracket and a second bracket; The first bracket is rotatably connected to the three-dimensional displacement stage; the first bracket is used to place the first focusing objective lens; The second bracket is rotatably connected to the three-dimensional translation stage; the second bracket is used to place the second focusing objective lens; Rotating the first bracket and the second bracket can adjust the angle of the Brillouin scattering signal.

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