Composition with sleep aiding effect
By using compositions of phosphatidylserine, gamma-aminobutyric acid, enzymatic oat flour and enzymatic pueraria powder, the dependence and addictive problems of existing sleep aid drugs are solved, and the sleep quality is improved through synergistic effects, achieving effective sleep aid effect without side effects.
Patent Information
- Application Number
- CN202411820368.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-11
- Publication Date
- 2025-04-08
AI Technical Summary
Existing sleep aid drugs have central nervous system inhibition, dependence and addictive problems. As the main raw material of health products, melatonin has the effect of aiding sleep aids and may inhibit the body's own secretory function, leading to endocrine system disorders.
A composition comprising phosphatidylserine, gamma-aminobutyric acid, enzymatic oat flour and enzymatic pueraria powder are provided, and the synergistic effect of these ingredients improves sleep quality.
This composition has no obvious side effects, and can effectively improve sleep quality, prolong sleep time, shorten sleep latency, and significantly reduce the number of autonomous activities and standing times in mice, indicating that it has a good sleep aid effect.
Smart Images
Figure CN119769726A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to a composition with sleep-aiding effect and a preparation method thereof. Background Art
[0002] The "China Sleep Research Report (2022)" pointed out that the Chinese people do not get enough sleep, with an average sleep duration of 7.06 hours per day, which is nearly 1.5 hours shorter than ten years ago. Compared with the survey statistics in the past decade, the average bedtime is now more than two hours later, and many people have difficulty falling asleep, which leads to insomnia.
[0003] Insomnia can lead to difficulty concentrating, decreased memory function, reduced work ability, and a significantly increased risk of cardiovascular and cerebrovascular diseases.
[0004] At present, the main drugs used to treat insomnia are benzodiazepines, zolpidem, amitriptyline and other drugs. The above drugs can cause central nervous system depression and cause dependence and addiction. Most health products with sleep-inducing functions use melatonin as the main raw material, but the efficacy of melatonin in helping sleep has yet to be verified. At the same time, since melatonin is a normal secretion of the human body, artificial supplementation of melatonin will inhibit the body's own secretion function, thereby causing endocrine disorders in the human body. Summary of the invention
[0005] The purpose of the present invention is to provide a composition with a sleep-aiding effect. The composition with a sleep-aiding effect has safe ingredients, scientific proportions, is easy to carry, has no obvious side effects, and has a good effect on improving sleep.
[0006] The invention provides a composition with a sleep-aiding effect, which comprises phosphatidylserine, gamma-aminobutyric acid, enzymolysis oatmeal powder and enzymolysis kudzu root powder.
[0007] The composition comprises 0.1-10 g of phosphatidylserine, 0.1-10 g of gamma-aminobutyric acid, 1.5-15 g of enzymatically hydrolyzed kudzu root powder, and 75-98.3 g of enzymatically hydrolyzed oatmeal powder per 100 g.
[0008] As a preferred embodiment, the composition comprises, by weight, 0.7-2.8 g of phosphatidylserine, 0.3-1.3 g of γ-aminobutyric acid, 1.5-6.3 g of enzymatically hydrolyzed kudzu root powder, and 89.6-97.4 g of enzymatically hydrolyzed oatmeal powder per 100 g.
[0009] Phosphatidylserine, abbreviated as PS, is a kind of ubiquitous phospholipids, usually located in the inner layer of the cell membrane. It is one of the cell membrane components and is related to a series of membrane functions. Especially in the human nervous system, it is one of the important components of the cell membrane of the brain. At the same time, it plays an important regulatory role in various brain functions, especially the stable regulation of brain memory and emotions. For example, it can affect the fluidity and permeability of the cell membrane, and can activate the metabolism and synthesis of various enzymes, thereby having a good repair effect on brain nerve cells.
[0010] γ-Aminobutyric acid, referred to as GABA, is an important inhibitory neurotransmitter in the central nervous system, which has good water solubility and thermal stability. It has been confirmed that GABA, as a small molecular weight non-protein amino acid, is safe for consumption and can be used in the production of beverages and other foods. Studies have shown that the intake of a certain amount of GABA has physiological effects such as improving the body's sleep quality and lowering blood pressure. The sedative and sleep-promoting effects of some drugs are because they can increase the affinity of GABA receptors to strengthen the binding of GABA to the recognition site. Some drugs can also increase the content of GABA in the brain by inhibiting its decomposition, and also increase the slow-wave sleep time to a certain extent.
[0011] Oats are rich in vitamins, including vitamin B1, vitamin B2, more vitamin E, niacin, folic acid, etc. Among them, vitamin B1 and B2 are higher than rice, and vitamin E is also higher than flour and rice. Oats are also rich in minerals, including calcium, iron, phosphorus, magnesium, zinc, copper, selenium, etc. In particular, the calcium content is significantly higher than wheat flour, rice, millet, buckwheat noodles, etc. The selenium content in oats is also high, equivalent to 3.72 times that of wheat, 7.9 times that of corn, and 34.8 times that of rice.
[0012] Enzymatic oats use amylase to hydrolyze the starch in oats, which can solve the problem of starch precipitation. At the same time, the polypeptides produced by enzymatic hydrolysis also have health benefits such as anti-oxidation, lowering blood lipids, lowering blood pressure, and lowering blood sugar.
[0013] Enzymatically hydrolyzed kudzu root powder is starch extracted from the tubers of Pueraria lobata (Willd.) Ohwi and P. thomsonii Benth., both of which are leguminous plants, by water grinding. Pueraria root powder contains more than 10 kinds of amino acids and more than 10 kinds of trace elements needed by the human body, and has certain anti-cancer and anti-cancer effects. Pueraria root powder treated with enzymatic hydrolysis technology retains effective chemical components and soluble cellulose to the maximum extent. The puerarin contained in it has the effects of promoting vascular microcirculation, penetrating the blood-brain barrier and protecting cerebral arteries, which can lower blood pressure, significantly increase the blood supply to ischemic tissues, and improve sleep quality.
[0014] The preparation method of the composition with sleep-aiding effect of the present invention is characterized by the following steps:
[0015] Step 1: Put the kudzu root tubers into a high-speed grinder and grind them into powder, wrap a proper amount of kudzu powder with defatted filter paper, put it in an ethanol solution with a mass concentration of 60% to 80%, and reflux extract it for 2 to 4 times, then add a certain amount of cellulase for enzymolysis, put it in a fume hood to air-dry after enzymolysis, and sieve it with a 150-mesh to 200-mesh filter sieve to obtain enzymolysis kudzu powder.
[0016] Step 2: Put the oats into a high-speed grinder and grind them into 40 mesh oatmeal powder, then stir them with high-temperature amylase and hydrolyze them to obtain enzymatic solution. After high-temperature sterilization and enzyme inactivation, vacuum concentrate them to solid form.
[0017] Step 3: phosphatidylserine, γ-aminobutyric acid, enzymatically hydrolyzed kudzu root powder and enzymatically hydrolyzed oatmeal powder are mixed in proportion to obtain a solid mixture. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 Effects of different samples on mouse body weight.
[0019] Figure 2 Subthreshold dose hypnosis experiment of sodium pentobarbital.
[0020] Figure 3 Experiment on prolonging the hypnotic time of sodium pentobarbital.
[0021] Figure 4 Sodium pentobarbital hypnotic latency test.
[0022] Figure 5 Mouse spontaneous activity experiment.
[0023] Figure 6 Mouse autonomous standing experiment. DETAILED DESCRIPTION
[0024] This embodiment provides a preparation method for a composition having a sleep-aiding effect. The composition comprises, by weight, phosphatidylserine, γ-aminobutyric acid, enzymatically hydrolyzed oatmeal powder, and enzymatically hydrolyzed kudzu root powder.
[0025] Embodiment 1
[0026] Step 1: Put the kudzu root tubers into a high-speed grinder and grind them into powder, wrap an appropriate amount of enzymatically hydrolyzed kudzu powder with defatted filter paper, place it in an ethanol solution with a mass concentration of 60%-80%, reflux extraction for 4-6 times, and then add a certain amount of cellulase for enzymolysis. After the enzymolysis is completed, put it in a fume hood to air dry, and sieve it with a 150-mesh to 200-mesh filter sieve to obtain the enzymatically hydrolyzed kudzu powder.
[0027] Step 2: Put the oats into a high-speed grinder and grind them into 40 mesh oatmeal powder, then stir them thoroughly with high-temperature amylase for enzymolysis to obtain enzymolysis solution. After high-temperature sterilization and enzyme inactivation, vacuum concentrate them to solid form.
[0028] Step 3: phosphatidylserine, γ-aminobutyric acid, enzymatically hydrolyzed kudzu root powder and enzymatically hydrolyzed oatmeal powder are mixed in proportion to obtain a composition mixture.
[0029] By weight, the composition contains 0.7 g of phosphatidylserine, 0.3 g of γ-aminobutyric acid, 1.6 g of enzymatically hydrolyzed kudzu root powder, and 97.4 g of enzymatically hydrolyzed oatmeal powder per 100 g.
[0030] Embodiment 2
[0031] The same production process as in Example 1 was adopted to prepare enzymatically hydrolyzed kudzu root powder and enzymatically hydrolyzed oatmeal powder.
[0032] By weight, the composition contains 2.8 g of phosphatidylserine, 1.3 g of gamma-aminobutyric acid, 6.3 g of enzymatically hydrolyzed kudzu root powder, and 89.6 g of enzymatically hydrolyzed oatmeal powder per 100 g.
[0033] Embodiment 3
[0034] The same production process as in Example 1 was adopted to prepare enzymatically hydrolyzed kudzu root powder and enzymatically hydrolyzed oatmeal powder.
[0035] By weight, the composition comprises 1.4 g of phosphatidylserine, 0.8 g of gamma-aminobutyric acid, 1.5 g of enzymatically hydrolyzed kudzu root powder, and 96.3 g of enzymatically hydrolyzed oatmeal powder per 100 g.
[0036] The beneficial effects of the present invention are specifically described below through test examples.
[0037] In order to observe the effect of the sleep-aiding composition prepared in the example on the sleep of mice, this experiment observed its sleep-aiding effect by extending the sodium pentobarbital sleep time test and the sodium pentobarbital sleep latency test.
[0038] Experimental animals: 40 SPF male Kunming mice, 1 week old, were given a 12-h light cycle, the breeding temperature was maintained at 21-27°C, and the air humidity was 40%-70%; the 40 mice were divided into 5 groups according to their body weight, and 4 mice in each group were tested separately, including a blank control group, a positive control group, and a low-concentration dose group, a medium-concentration dose group, and a high-concentration dose group of the combination sample, a total of 5 experimental groups of mice.
[0039] Sampling route: intragastric administration.
[0040] Sample dosage: Combined with the recommended dosage standard for humans, the dosage was calculated and given to mice according to the following dosages: phosphatidylserine group (0.03-0.06 g / kg.bw), γ-aminobutyric acid (0.01-0.05 g / kg.bw), and enzymatically hydrolyzed kudzu root powder (0.4-1.6 g / kg.bw).
[0041] 7.5g, 15g, and 30g of the test substance were respectively dissolved in 100ml of sterile water to obtain the water-soluble substance of the embodiment for functional test, namely, the low-dose group (0.75g / kg.bw) of the test substance, the medium-dose group (1.5g / kg.bw) of the test substance, and the high-dose group (3.0g / kg.bw) of the test substance.
[0042] Dosing volume: 10ml / kg.bw.
[0043] (1) Effects of the test substances on the body weight of mice
[0044] The data before the last oral administration of the mice were recorded, and the weight of the mice in the control group was compared with that of the mice in the other experimental groups. Figure 1 .
[0045] Note: *p<0.05 **p<0.01 compared with the control group.
[0046] Figure 1 The results showed that compared with the control group, there was no significant change in the body weight of mice in the positive control group and the low-, medium- and high-dose groups of the test substance.
[0047] (2) Pentobarbital sodium subthreshold sleep dose test
[0048] The subthreshold sleep dose refers to the maximum dose at which 100% of the mice's righting reflex does not disappear. Figure 2 .
[0049] Figure 2 The results showed that the incidence of sleep in each experimental group was higher than that in the control group, and gradually increased with the increase of the concentration of the composition sample. The results showed that the composition may achieve a synergistic sleep effect with sodium pentobarbital by affecting the metabolism of sodium pentobarbital.
[0050] (3) Prolonged pentobarbital sodium sleep time test
[0051] 30 minutes after the animals were last given a certain dose of pure water and different samples, sodium pentobarbital (200 mg / kg) was injected intraperitoneally, and the animals were immediately observed and timed. The disappearance of the righting reflex was used as the indicator of falling asleep, and the sleeping time of each mouse was recorded (i.e., from the disappearance of the righting reflex to the recovery of the righting reflex). Figure 3 .
[0052] Note: *p<0.05, **p<0.01 compared with the control group.
[0053] Figure 3The results showed that compared with the control group, the sleep time of the positive control group was extended by 340.81%, while the average sleep time of mice in the low, medium and high dose groups of the combination was extended by 75.23%, 121.56% and 170.88% respectively. As the concentration of the drug was increased, the sleep time of the mice was also extended. This shows that the combination will produce a certain synergistic effect with sodium pentobarbital and extend the sleep time of mice.
[0054] (4) Pentobarbital sodium sleep latency test
[0055] 30 minutes after the animals were last given a certain dose of pure water and different samples, sodium pentobarbital (200 mg / kg) was intraperitoneally injected, and the animals were immediately observed and timed. The disappearance of the righting reflex was used as an indicator to record the sleep latency of each mouse (i.e., from the injection of sodium pentobarbital to the disappearance of the righting reflex). Figure 4 .
[0056] Note: *p<0.05, **p<0.01 compared with the control group.
[0057] Figure 4 The results showed that the sleep latency of mice in the positive control group was shortened compared with the control group. The sleep latency of mice in the high oat milk group was also significantly shortened, but the effects of the medium and low doses of oat milk on the sleep latency induced by sodium pentobarbital were not obvious. The results showed that oat milk may have a synergistic effect with sodium pentobarbital, helping to reduce the sleep latency of mice.
[0058] The experiment further verified that enzymatically hydrolyzed kudzu root powder can relieve fatigue by promoting blood circulation; γ-aminobutyric acid can directly help sleep; and phosphatidylserine can also indirectly promote effective sleep. This shows that the proper combination of enzymatically hydrolyzed kudzu root powder, γ-aminobutyric acid, and phosphatidylserine has a certain synergistic effect, which further shows that the test substance can shorten the sleep latency to a certain extent.
[0059] (5) Mouse autonomous activity experiment
[0060] The more activities and the more frequent standing times, the more active the mouse's behavior is, which means that the mouse's central nervous system is more excited. By comparing the changes in the activity levels of mice before and after drug administration, the sedative effect of the drug can be evaluated. Figure 5 , 6 .
[0061] result Figure 5 and 6 The results showed that after the mice were treated with the composition samples, the number of activities and standing times of the mice in the positive control group and the low, medium and high concentration composition sample groups decreased compared with the blank control group. This indicates that the oat composition sample can produce some sedative effects on the activities of the mice, and the number of autonomous activities of the mice is reduced.
[0062] The experimental results showed that after the test substance was continuously gavaged into mice for 30 days, the results of the four experiments, namely "pentobarbital sodium subthreshold dose hypnosis test", "pentobarbital sodium prolonged sleep time test", "pentobarbital sodium sleep latency test" and "mouse autonomous activity test", were all positive, and no abnormalities were found in the mice during the experimental observation.
Claims
1. A composition having a sleep-aiding effect, characterized in that: It contains phosphatidylserine, gamma-aminobutyric acid, enzymatically hydrolyzed oatmeal powder, and enzymatically hydrolyzed kudzu root powder; The composition comprises 0.1-10 g of phosphatidylserine, 0.1-10 g of gamma-aminobutyric acid, 1.5-15 g of enzymatically hydrolyzed kudzu root powder, and 75-98.3 g of enzymatically hydrolyzed oatmeal powder per 100 g.
2. The composition according to claim 1, characterized in that The composition comprises 0.7-2.8 g of phosphatidylserine, 0.3-1.3 g of gamma-aminobutyric acid, 1.5-6.3 g of enzymatically hydrolyzed kudzu root powder, and 89.6-97.4 g of enzymatically hydrolyzed oatmeal powder per 100 g.
3. The method for preparing the composition according to claim 1, characterized in that: The steps include: Step 1: Put the kudzu root tubers into a high-speed grinder and grind them into powder, wrap a proper amount of kudzu powder with defatted filter paper, put it into an ethanol solution with a mass concentration of 60% to 80%, and reflux extract it for 2 to 4 times, then add a certain amount of cellulase for enzymolysis, put it in a fume hood to air-dry after enzymolysis, and sieve it with a 150-mesh to 200-mesh filter sieve to obtain enzymolysis kudzu powder; Step 2: Put the oats into a high-speed grinder and grind them into 40 mesh oatmeal powder, then stir them thoroughly with high-temperature amylase and perform enzymolysis to obtain enzymolysis solution. After high-temperature sterilization and enzyme inactivation, vacuum concentrate them to a solid state; Step 3: phosphatidylserine, γ-aminobutyric acid, enzymatically hydrolyzed kudzu root powder and enzymatically hydrolyzed oatmeal powder are mixed in proportion to obtain a solid mixture.