A cell scaffold for promoting bone tissue regeneration in the femoral head necrosis area

Abstract

The present invention discloses a cell scaffold for promoting bone tissue regeneration in the femoral head necrosis region, wherein a drug with mesenchymal stem cells and bone marrow nucleated cells as effective ingredients is attached to the cell scaffold; wherein the concentration of the mesenchymal stem cells is 5×10 5 ‑1×10 6 / cm 3 The concentration of bone marrow nucleated cells is 5×10 5 ‑1×10 6 / cm 3 The cell scaffold for promoting bone tissue regeneration in the necrotic area of ​​the femoral head of the present invention can significantly improve the blood supply in the femoral head of the femoral head necrosis, reduce the formation of the necrotic area, promote bone regeneration in the necrotic area, improve the bone density in the necrotic area, and provide a new treatment strategy for the treatment of femoral head necrosis.

Description

Technical Field

[0001] The present invention relates to the field of biomedical technology, and in particular to a cell scaffold for promoting bone tissue regeneration in a femoral head necrosis region. Background Art

[0002] Avascular Necrosis of the Femoral Head (ANFH), also known as avascular necrosis or avascular necrosis of the femoral head, is a disease in which bone cells die due to insufficient blood supply to the femoral head. As the disease progresses, the femoral head may gradually collapse, causing hip pain and dysfunction. The treatment of ANFH depends on the severity of the disease, the extent of the lesion, and the individual condition of the patient. Currently, the methods for treating femoral head necrosis are mainly divided into two categories: conservative treatment and surgical treatment. Patients with advanced femoral head necrosis require joint replacement, but this surgery carries the risk of prosthesis revision, prosthesis loosening, and even catastrophic periprosthetic infection.

[0003] Therefore, studying stem cell therapy for the treatment of femoral head necrosis is a technical problem that technical personnel in this field urgently need to solve. Summary of the invention

[0004] The purpose of the present invention is to provide a cell scaffold for promoting bone tissue regeneration in the necrosis area of ​​the femoral head in view of the deficiencies in the prior art.

[0005] To achieve the above object, the technical solution adopted by the present invention is:

[0006] Provided is a cell scaffold for promoting bone tissue regeneration in a femoral head necrosis region, wherein a drug having mesenchymal stem cells and bone marrow nucleated cells as effective ingredients is attached to the cell scaffold; the concentration of the mesenchymal stem cells is 5×10 5 -1×10 6 / cm 3 The concentration of bone marrow nucleated cells is 5×10 5 -1×10 6 / cm 3 .

[0007] Furthermore, the cell scaffold is made of demineralized calf bone.

[0008] Furthermore, the mesenchymal stem cells are mesenchymal cells derived from human amniotic tissue.

[0009] Furthermore, the bone marrow nucleated cells are autologous bone marrow nucleated cells.

[0010] The present invention adopts the above technical solution, and has the following technical effects compared with the prior art:

[0011] The cell scaffold for promoting bone tissue regeneration in the necrotic area of ​​the femoral head of the present invention can significantly improve the blood supply in the femoral head of the femoral head necrosis, reduce the formation of necrotic areas, promote bone regeneration in the necrotic area, improve the bone density in the necrotic area, and provide a new treatment strategy for treating femoral head necrosis. DETAILED DESCRIPTION

[0012] The present invention is further described below in conjunction with specific embodiments, but is not intended to be limiting of the present invention. It should be noted that the embodiments and features in the embodiments of the present invention may be combined with each other without conflict.

[0013] Example 1

[0014] This embodiment provides a cell scaffold for promoting bone tissue regeneration in the femoral head necrosis area. The cell scaffold is attached with a drug with mesenchymal stem cells and bone marrow nucleated cells as active ingredients. The concentration of the mesenchymal stem cells is 5×10 5 / cm 3 The concentration of bone marrow nucleated cells is 5×10 5 / cm 3 .

[0015] Among them, the cell scaffold is made of demineralized calf bone.

[0016] Among them, mesenchymal stem cells are mesenchymal cells derived from human amniotic tissue.

[0017] The bone marrow nucleated cells are autologous bone marrow nucleated cells.

[0018] Example 2

[0019] This embodiment provides a cell scaffold for promoting bone tissue regeneration in the femoral head necrosis area. The cell scaffold is attached with a drug with mesenchymal stem cells and bone marrow nucleated cells as effective ingredients. The concentration of the mesenchymal stem cells is 8×10 5 / cm 3 The concentration of bone marrow nucleated cells is 8×10 5 / cm 3 .

[0020] Among them, the cell scaffold is made of demineralized calf bone.

[0021] Among them, mesenchymal stem cells are mesenchymal cells derived from human amniotic tissue.

[0022] The bone marrow nucleated cells are autologous bone marrow nucleated cells.

[0023] Example 3

[0024] This embodiment provides a cell scaffold for promoting bone tissue regeneration in the femoral head necrosis area. The cell scaffold is attached with a drug with mesenchymal stem cells and bone marrow nucleated cells as active ingredients. The concentration of the mesenchymal stem cells is 1×10 6 / cm 3 The concentration of bone marrow nucleated cells is 1×10 6 / cm 3 .

[0025] Among them, the cell scaffold is made of demineralized calf bone.

[0026] Among them, mesenchymal stem cells are mesenchymal cells derived from human amniotic tissue.

[0027] The bone marrow nucleated cells are autologous bone marrow nucleated cells.

[0028] Example 4 Animal Experiment

[0029] 1) Experimental animals and model establishment

[0030] Experimental animals: Ordinary one-month-old rats weighing about 250 g were selected, regardless of gender, for a total of 120 rats.

[0031] Model establishment: Dexamethasone was injected into the bone margin of the left leg of rats for 2 weeks to induce ischemic femoral head necrosis model.

[0032] 2) Surgical treatment

[0033] Anesthesia and fixation:

[0034] The rats were anesthetized and after confirmation of successful anesthesia, the rats were fixed on the operating table in a prone position.

[0035] Cell scaffold in vivo transplantation:

[0036] Control group: no treatment was given.

[0037] Experimental group: conventional osteonecrosis surgery was performed to scrape away the necrotic bone, expose the normal bone tissue, and form a bone defect area; the cell scaffolds of Examples 1-3 were implanted into the bone defect area, and then sutured surgically.

[0038] 3) Treatment cycle

[0039] Treatment time: Continue observation for 4 weeks after surgical treatment.

[0040] 4) Sample collection and measurement

[0041] Femur collection: After 4 weeks of treatment, the rats were sacrificed and the left and right femurs were dissected.

[0042] Determination indicators:

[0043] Femur weight: Measure and record the weight of the left and right femur of each rat.

[0044] Bone density measurement: Use appropriate equipment (such as dual-energy X-ray absorptiometry DXA) to measure the bone density of the femur.

[0045] The experimental results are shown in Table 1 below.

[0046] Table 1

[0047] Group Femoral head weight (g) <![CDATA[Bone density (g / cm 2 ) <!-- 2 -->]]> Example 1 0.785 0.1056 Example 2 0.812 0.1160 Example 3 0.805 0.1102 Control group 0.435 0.0980

[0048] It can be seen from Table 1 that the cell scaffold of the present invention can well repair femoral head defects, and the femoral weight and bone density of the treated rats are higher than those of the untreated control group.

[0049] The above description is only a preferred embodiment of the present invention, and does not limit the implementation mode and protection scope of the present invention. For those skilled in the art, it should be aware that all solutions obtained by equivalent substitutions and obvious changes made using the contents of the specification of the present invention should be included in the protection scope of the present invention.

Claims

1. A cell scaffold for promoting bone tissue regeneration in the femoral head necrosis area, characterized in that: The cell scaffold is attached with a drug having mesenchymal stem cells and bone marrow nucleated cells as active ingredients; the concentration of the mesenchymal stem cells is 5×10 5 -1×10 6 / cm 3 The concentration of bone marrow nucleated cells is 5×10 5 -1×10 6 / cm 3 .

2. The cell scaffold for promoting bone tissue regeneration in the femoral head necrosis area according to claim 1, characterized in that: The cell scaffold is made of demineralized calf bone.

3. The cell scaffold for promoting bone tissue regeneration in the femoral head necrosis area according to claim 1, characterized in that: The mesenchymal stem cells are mesenchymal cells derived from human amniotic tissue.

4. The cell scaffold for promoting bone tissue regeneration in the femoral head necrosis area according to claim 1, characterized in that: The bone marrow nucleated cells are autologous bone marrow nucleated cells.