Micro-fluidic chip device for functional research of placenta bile acid transporter

Abstract

The invention discloses a micro-fluidic chip device for functional research of a placenta bile acid transporter, and relates to the technical field of research of placenta cell bile acid transporters, the micro-fluidic chip device comprises a table body, four corners of the bottom of the table body are provided with pillars; the bracket is fixed at the top of the table body, and a plurality of chip mechanisms are arranged at the top of the bracket; and the plurality of conveying assemblies are fixed at the top of the table body at equal intervals, and the plurality of conveying assemblies are in one-to-one correspondence with the plurality of chip mechanisms. Through work of the power assembly, the transmission mechanism and the pneumatic assembly, the first pistons in the first barrel and the second barrel can ascend and descend, and then placenta cells can reciprocate in the chip mechanism, so that the placenta cells make full contact with the two faces of a biological membrane of the chip mechanism, and the placenta cells can be separated from the chip mechanism. Therefore, the cell culture efficiency is improved, and the overall experiment efficiency is further improved.

Description

Technical Field

[0001] The invention relates to the technical field of placental cell bile acid transporter research, and in particular to a microfluidic chip device for placental bile acid transporter function research. Background Art

[0002] A microfluidic chip is a device that uses tiny channels and microfluidic control technology to process and analyze liquids. Its core principle is to precisely control the flow, mixing, reaction, and separation of fluids at the micron or nanoscale.

[0003] When conducting research on the function of bile acid transporters in placental cells, it is generally necessary to culture placental cells in a microfluidic chip and distribute them in a membrane-like manner in the chip, and then inject a solution containing bile acid into the microchannel on one side of the membrane in the chip. The transporters in the placental cells on the biological membrane will transport bile acid to the microchannel on the other side of the membrane. By measuring the bile acid concentrations in the two microchannels, the transport capacity of the bile acid transporters in the placental cells can be determined.

[0004] In the prior art, most microfluidic chips generally continuously inject cell solutions during cell culture to ensure that cells can adhere to specific areas in the microfluidic chip. During the continuous flow process, cells are easily detached from the microfluidic chip, and during the cell culture process, the cell concentration in a specific area is uncontrollable. Therefore, in experiments based on microfluidic chips, culturing cells leads to a decrease in the overall experimental efficiency. Summary of the invention

[0005] The purpose of the present invention is to provide a microfluidic chip device for studying the function of placental bile acid transporters to solve the technical problems in the prior art.

[0006] The present invention provides a microfluidic chip device for studying the function of placental bile acid transporters, comprising:

[0007] A platform body, wherein pillars are arranged at the four corners of the bottom of the platform body;

[0008] A bracket, the bracket is fixed on the top of the platform, and a plurality of chip mechanisms are arranged on the top of the bracket;

[0009] A plurality of conveying components, wherein the plurality of conveying components are fixed at equal distances on the top of the platform, and the plurality of conveying components correspond to the plurality of chip mechanisms one by one;

[0010] A power assembly, the power assembly is fixed to the bottom of the platform, and the power assembly is connected to a plurality of conveying assemblies;

[0011] Wherein, the conveying component comprises:

[0012] Two first cylinders, the two first cylinders are respectively fixed at two sides of the top of the platform, and the two first cylinders are both connected to the chip mechanism, and the two first cylinders are used to store bile acid solution;

[0013] Two first tubes, the two first tubes are respectively fixed at two sides of the top of the platform; the two first tubes are both connected to the chip mechanism, the two first tubes are used to store the placental cell solution, and a first piston is slidably arranged in each of the first cylinders and each of the first tubes;

[0014] Four transmission mechanisms, the four transmission mechanisms are all fixed on the bottom of the platform, and the four transmission mechanisms correspond one to one with the two first cylinders and the two first tubes;

[0015] A pneumatic assembly is fixed at the bottom of the platform, and the pneumatic assembly is connected to the power assembly, and the pneumatic assembly is communicated with four transmission mechanisms.

[0016] Preferably, the chip mechanism comprises:

[0017] A chip, wherein the chip is fixed on the top of the bracket;

[0018] Two microchannels, both of which are opened in the chip, the two microchannels are connected, and the two microchannels are distributed up and down;

[0019] The biofilm is arranged in the chip, and the biofilm is located between two microchannels.

[0020] Preferably, the conveying assembly further comprises:

[0021] Two first tubes, the two first tubes are respectively connected to the two first cylinders, and the ends of the two first tubes are respectively connected to the two ends of the microchannel below;

[0022] Two first solenoid valves, the two first solenoid valves are respectively arranged through the tops of the two second cylinders;

[0023] Two second tubes, the two second tubes are respectively connected to the two first solenoid valves, and the two second tubes are respectively connected to two ends of the microchannel located above;

[0024] Two second solenoid valves, both of which are fixed through the upper side walls of the two first cylinders and the second cylinders on the same side of the chip;

[0025] Two third solenoid valves are both fixed through the lower side walls of the two first cylinders and the second cylinder on the other side of the chip.

[0026] Preferably, the transmission mechanism comprises:

[0027] A first sleeve, the first sleeve is fixed to the bottom of the platform, and a second piston is slidably arranged in the first sleeve;

[0028] A transmission rod is fixed on the top of the second piston and passes through the top of the sliding platform. The top of the transmission rod is fixed to the first piston nearby.

[0029] Preferably, the pneumatic assembly comprises:

[0030] Two second sleeves, both of which are fixed to the bottom of the platform;

[0031] Two third pistons, the two third pistons are respectively slidably disposed in two second sleeves, and each of the second sleeves is fixed with a connecting block;

[0032] Two tee pipes, both of which are connected to the second sleeve, and the other two ends of the two tee pipes are respectively connected to two adjacent first sleeves;

[0033] A first connecting unit and a second connecting unit, wherein the first connecting unit and the second connecting unit are connected to two connecting blocks respectively.

[0034] Preferably, the power assembly comprises:

[0035] A door frame, wherein the door frame is fixed to the bottom of the platform;

[0036] A plurality of motors, wherein the plurality of motors are fixed at the bottom of the portal frame;

[0037] A plurality of crankshafts are rotatably arranged at the bottom of the platform, and the plurality of crankshafts and the output shafts of the plurality of motors are fixed one to one.

[0038] Preferably, the first connecting unit includes:

[0039] A connecting rod is rotatably connected to one of the connecting blocks, and the connecting rod is rotatably connected to a nearby crankshaft.

[0040] Preferably, the second connecting unit comprises:

[0041] A sleeve rod, the sleeve rod being rotatably connected to another connecting block;

[0042] A fourth piston, the fourth piston being fixed to the end of the sleeve rod;

[0043] A mounting block, a third sleeve being fixed to a side wall of the mounting block, and a fourth piston being slidably disposed in the third sleeve, and the mounting block being rotationally connected to a nearby crankshaft;

[0044] A valve is provided through the side wall of the third sleeve.

[0045] Compared with the prior art, the beneficial effects of the present invention are:

[0046] The present invention, through the provision of the first cylinder, the second cylinder, the power assembly and the transmission mechanism, can realize that when performing cell culture work, the first piston in the first cylinder and the second cylinder can rise and fall through the operation of the power assembly, the transmission mechanism and the pneumatic assembly, thereby realizing the reciprocating movement of placental cells in the chip mechanism, so that the placental cells and the two sides of the biofilm of the chip mechanism can be fully contacted, thereby improving the efficiency of cell culture and thus improving the overall experimental efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0048] Figure 1 It is a three-dimensional structural schematic diagram of the present invention;

[0049] Figure 2 It is a schematic diagram of the structure of the portal frame and the motor of the present invention;

[0050] Figure 3 It is a schematic diagram of the structure of the second solenoid valve and the third solenoid valve and the first cylinder and the second cylinder of the present invention;

[0051] Figure 4 It is a schematic diagram of the first sleeve and the tee structure of the present invention;

[0052] Figure 5 is a schematic cross-sectional structural diagram of a second sleeve of the present invention;

[0053] Figure 6 It is a schematic diagram of the explosion structure of the sleeve rod and the third sleeve of the present invention;

[0054] Figure 7 It is a schematic diagram of the cross-sectional structure of the chip of the present invention.

[0055] Reference numerals:

[0056] 1. Platform; 2. Pillar; 3. Bracket; 4. Chip; 5. First cylinder; 6. First tube; 7. Second cylinder; 8. Second tube; 9. First solenoid valve; 10. Second solenoid valve; 11. First sleeve; 12. Three-way pipe; 13. Door frame; 14. Motor; 15. Second sleeve; 16. Third solenoid valve; 17. Transmission rod; 18. First piston; 19. Second piston; 20. Crankshaft; 21. Third piston; 22. Connecting block; 23. Connecting rod; 24. Mounting block; 25. Third sleeve; 26. Valve; 27. Fourth piston; 28. Sleeve rod; 29. ​​Microchannel; 30. Biofilm. DETAILED DESCRIPTION

[0057] The technical solution of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0058] The components of the embodiments of the present invention generally described and shown in the drawings herein may be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention.

[0059] Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without making any creative work shall fall within the scope of protection of the present invention.

[0060] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0061] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0062] Combine the following Figures 1 to 7As shown, an embodiment of the present invention provides a microfluidic chip device for studying the function of placental bile acid transporters, comprising:

[0063] The platform body 1 has pillars 2 disposed at the four corners of the bottom of the platform body 1;

[0064] A bracket 3, the bracket 3 is fixed on the top of the platform 1, and a plurality of chip mechanisms are arranged on the top of the bracket 3;

[0065] Multiple conveying components, the multiple conveying components are fixed at equal distances on the top of the platform 1, and the multiple conveying components correspond to the multiple chip mechanisms one by one;

[0066] A power assembly, which is fixed at the bottom of the platform 1 and is connected to a plurality of conveying assemblies;

[0067] The conveying components include:

[0068] Two first cylinders 5, the two first cylinders 5 are respectively fixed at two sides of the top of the platform 1, and the two first cylinders 5 are both connected to the chip mechanism, and the two first cylinders 5 are used to store bile acid solution;

[0069] Two first tubes 6, the two first tubes 6 are respectively fixed at both sides of the top of the platform 1; and the two first tubes 6 are connected to the chip mechanism, and the two first tubes 6 are used to store the placental cell solution, and a first piston 18 is slidably arranged in each first cylinder 5 and each first tube 6;

[0070] Four transmission mechanisms, all of which are fixed to the bottom of the platform 1, and the four transmission mechanisms correspond to the two first cylinders 5 and the two first tubes 6 one by one;

[0071] Pneumatic assembly: the pneumatic assembly is fixed at the bottom of the platform 1, and the pneumatic assembly is connected to the power assembly, and the pneumatic assembly is communicated with four transmission mechanisms.

[0072] The power component drives the pneumatic component to work, and the transmission mechanism can drive the first pistons 18 in the two first cylinders 5 and the two first cylinders 6 to work, so that the solution rich in placental cells in the first cylinder 5 and the second cylinder 6 enters the chip mechanism, thereby increasing the culture of placental cells in the chip mechanism and improving the overall experimental efficiency.

[0073] Furthermore, the chip structure includes:

[0074] Chip 4, chip 4 is fixed on the top of bracket 3;

[0075] Two microchannels 29, both of which are provided in the chip 4, the two microchannels 29 are connected, and the two microchannels 29 are distributed up and down;

[0076] The biofilm 30 is disposed in the chip 4 , and the biofilm 30 is located between the two microchannels 29 .

[0077] Furthermore, the conveying assembly also includes:

[0078] Two first tube bodies 6, the two first tube bodies 6 are respectively connected to the two first cylinder bodies 5, and the ends of the two first tube bodies 6 are respectively connected to the two ends of the microchannel 29 located below;

[0079] Two first solenoid valves 9, which are respectively arranged through the tops of the two second cylinders 7;

[0080] Two second tube bodies 8, the two second tube bodies 8 are respectively connected to the two first solenoid valves 9, and the two second tube bodies 8 are respectively connected to two ends of the microchannel 29 located above;

[0081] Two second solenoid valves 10, both of which are fixed through the upper side walls of the two first cylinders 5 and the second cylinders 7 on the same side of the chip 4;

[0082] Two third solenoid valves 16 are both fixed through and below the side walls of the two first cylinders 5 and the second cylinders 7 on the other side of the chip 4 .

[0083] Through the control of the second solenoid valve 10 and the third solenoid valve 16, the solution in the first cylinder 5 and the second cylinder 6 can be injected and output, so as to facilitate the cell culture work and the detection work.

[0084] Furthermore, the transmission mechanism comprises:

[0085] A first sleeve 11, the first sleeve 11 is fixed to the bottom of the platform 1, and a second piston 19 is slidably arranged in the first sleeve 11;

[0086] The transmission rod 17 is fixed on the top of the second piston 19 , and the transmission rod 17 passes through the top of the sliding platform body 1 , and the top of the transmission rod 17 is fixed to the first piston 18 nearby.

[0087] Further, the pneumatic assembly includes:

[0088] Two second sleeves 15, both of which are fixed to the bottom of the platform 1;

[0089] Two third pistons 21, the two third pistons 21 are slidably disposed in the two second sleeves 15 respectively, and each second sleeve 15 is fixed with a connecting block 22;

[0090] Two three-way pipes 12, both of which are connected to the second sleeve 15, and the other two ends of the two three-way pipes 12 are respectively connected to two adjacent first sleeves 11;

[0091] The first connecting unit and the second connecting unit are connected to two connecting blocks 22 respectively.

[0092] Furthermore, the power assembly includes:

[0093] A door frame 13, which is fixed to the bottom of the platform 1;

[0094] A plurality of motors 14, wherein the plurality of motors 14 are fixed at the bottom of the door frame 13;

[0095] A plurality of crankshafts 20 are rotatably disposed at the bottom of the platform 1 , and the plurality of crankshafts 20 and the output shafts of the plurality of motors 14 are fixed one to one.

[0096] The switch of the motor 14 is turned on. When the motor 14 is working, the crankshaft 20 is driven to rotate, and the corresponding third piston 21 is driven to reciprocate in the second sleeve 15 through the mounting block 24, the third sleeve 25, the sleeve rod 28 and another connecting block 22;

[0097] During the movement of the third piston 21 in the second sleeve 15, gas is periodically injected into and extracted from the gases in the two first sleeves 11 through the three-way pipe 12, so that the second piston 19 in the first sleeve 11 reciprocates up and down, and then the first piston 18 is driven to reciprocate up and down through the action of the transmission rod 17.

[0098] Furthermore, the first connection unit includes:

[0099] The connecting rod 23 is rotatably connected to one of the connecting blocks 22 , and the connecting rod 23 is rotatably connected to the nearest crankshaft 20 .

[0100] Further, the second connection unit includes:

[0101] A sleeve rod 28, the sleeve rod 28 is rotatably connected to another connecting block 22;

[0102] A fourth piston 27, the fourth piston 27 is fixed to the end of the sleeve rod 28;

[0103] A mounting block 24, a third sleeve 25 is fixed to the side wall of the mounting block 24, and a fourth piston 27 is slidably disposed in the third sleeve 25, and the mounting block 24 is rotatably connected to the nearest crankshaft 20;

[0104] The valve 26 is disposed through the side wall of the third sleeve 25 .

[0105] The valve 26 is closed. Since the valve 26 is closed, the sliding between the third sleeve 25 and the fourth piston 27 is subject to air resistance, so that the sliding distance between the third sleeve 25 and the sleeve rod 28 is limited. Since the third sleeve 25 and the sleeve rod 28 are almost unable to slide relative to each other at this time, during the rotation of the crankshaft 20, the corresponding third piston 21 is driven to reciprocate in the second sleeve 15 through the connecting rod 23 and one of the connecting blocks 22;

[0106] When the valve 26 is turned on, since external gas can be exchanged with the third sleeve 25 through the valve 26, the sleeve rod 28 and the third sleeve 25 can be contracted, and then during the rotation of the crankshaft 20, only the third piston 21 in the second sleeve 15 can reciprocate.

[0107] The specific working method is as follows: first, the placental cells are cultivated. At this time, the switches of the two first electromagnetic valves 9 are turned on, and the solution containing the placental cells is injected into the two first cylinders 5 and the two second cylinders 7 through the third electromagnetic valve 16 and the second electromagnetic valve 10, and the switch of the valve 26 is closed. Since the valve 26 is closed, the sliding between the third sleeve 25 and the fourth piston 27 is subject to the resistance of the air, so that the sliding distance between the third sleeve 25 and the sleeve rod 28 is limited;

[0108] When the motor 14 is turned on, the motor 14 drives the crankshaft 20 to rotate. Since the third sleeve 25 and the sleeve rod 28 are almost unable to slide relative to each other at this time, during the rotation of the crankshaft 20, the corresponding third piston 21 is driven to reciprocate in the second sleeve 15 through the connecting rod 23 and one of the connecting blocks 22, and the corresponding third piston 21 is driven to reciprocate in the second sleeve 15 through the mounting block 24, the third sleeve 25, the sleeve rod 28 and the other connecting block 22;

[0109] During the movement of the third piston 21 in the second sleeve 15, gas is periodically injected into and extracted from the gases in the two first sleeves 11 through the three-way pipe 12, so that the second piston 19 in the first sleeve 11 reciprocates up and down, and then the first piston 18 is driven to reciprocate up and down through the action of the transmission rod 17;

[0110] Then, the solutions containing placental cells in the two first cylinders 5 and the two second cylinders 7 enter the two microchannels 29 of the chip 4, and the solutions containing placental cells are pushed in the two microchannels 29, so that the placental cells can fully contact the surface of the biofilm 30, so as to ensure that the placental cells can quickly attach to the surface of the biofilm 30;

[0111] After the placental cells fully grow on the two surfaces of the biofilm 30, the two first solenoid valves 9 are closed, and the solution containing bile acid is injected through the third solenoid valve 16 on the first cylinder 5, and the switch of the valve 26 is turned on. Since the external gas can be exchanged with the third sleeve 25 through the valve 26, the sleeve rod 28 and the third sleeve 25 can be contracted, and then during the rotation of the crankshaft 20, only the third piston 21 in the second sleeve 15 can reciprocate, so that the first piston 18 in the first cylinder 5 can reciprocate up and down, and cooperate with the continuously input bile acid solution, so that the bile acid solution can pass through one of the microchannels 29 at a constant speed, wherein when the bile acid passes through the biofilm 30, the placental cells on the biofilm 30 transport the bile acid, so that the bile acid concentration in the other microchannel 29 increases;

[0112] During the collection, the bile acid solution outputted from one of the microchannels 29 is collected, and the change in bile acid concentration is measured. Another microchannel 29 is flushed with physiological saline or the like, and the bile acid concentration in the microchannel 29 is measured.

[0113] Through multiple chip structures, multiple sets of data can be collected simultaneously to ensure the accuracy of the experimental results.

[0114] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A microfluidic chip device for studying the function of placental bile acid transporters, characterized in that: include: A platform (1), wherein pillars (2) are arranged at four corners of the bottom of the platform (1); A bracket (3), the bracket (3) being fixed on the top of the platform (1), and a plurality of chip mechanisms being arranged on the top of the bracket (3); A plurality of conveying components, wherein the plurality of conveying components are fixed at equal distances on the top of the platform (1), and the plurality of conveying components correspond to the plurality of chip mechanisms one by one; A power assembly, the power assembly is fixed to the bottom of the platform (1), and the power assembly is connected to a plurality of conveying assemblies; Wherein, the conveying component comprises: Two first cylinders (5), the two first cylinders (5) are respectively fixed on both sides of the top of the platform (1), and the two first cylinders (5) are both connected to the chip mechanism, and the two first cylinders (5) are used to store bile acid solution; Two first tubes (6), the two first tubes (6) are respectively fixed on both sides of the top of the platform (1); and the two first tubes (6) are both connected to the chip mechanism, and the two first tubes (6) are used to store the placental cell solution, and a first piston (18) is slidably arranged in each of the first cylinders (5) and each of the first tubes (6); Four transmission mechanisms, the four transmission mechanisms are all fixed on the bottom of the platform (1), and the four transmission mechanisms correspond one to one with the two first cylinders (5) and the two first tubes (6); A pneumatic assembly is fixed at the bottom of the platform (1), the pneumatic assembly is connected to the power assembly, and the pneumatic assembly is connected to four transmission mechanisms.

2. A microfluidic chip device for studying the function of placental bile acid transporters according to claim 1, characterized in that: The chip structure comprises: A chip (4), wherein the chip (4) is fixed on the top of the bracket (3); Two microchannels (29), both of which are disposed in the chip (4), the two microchannels (29) are connected to each other, and the two microchannels (29) are distributed vertically; A biofilm (30) is disposed in the chip (4), and the biofilm (30) is located between two microchannels (29).

3. A microfluidic chip device for studying the function of placental bile acid transporters according to claim 2, characterized in that: The conveying assembly also includes: Two first tubes (6), the two first tubes (6) are respectively connected to the two first cylinders (5), and the ends of the two first tubes (6) are respectively connected to the two ends of the microchannel (29) located below; Two first solenoid valves (9), the two first solenoid valves (9) are respectively arranged through the tops of the two second cylinders (7); Two second tubes (8), the two second tubes (8) are respectively connected to the two first solenoid valves (9), and the two second tubes (8) are respectively connected to two ends of the microchannel (29) located above; Two second solenoid valves (10), each of the two second solenoid valves (10) being fixed through and above the side walls of the two first cylinders (5) and the second cylinder (7) on the same side of the chip (4); Two third solenoid valves (16), both of which are fixed through the lower side walls of the two first cylinders (5) and the second cylinder (7) on the other side of the chip (4).

4. A microfluidic chip device for studying the function of placental bile acid transporters according to claim 1, characterized in that: The transmission mechanism comprises: A first sleeve (11), the first sleeve (11) being fixed to the bottom of the platform (1), and a second piston (19) being slidably disposed in the first sleeve (11); A transmission rod (17) is fixed on the top of the second piston (19), and the transmission rod (17) passes through the top of the sliding platform (1), and the top end of the transmission rod (17) is fixed to the nearest first piston (18).

5. A microfluidic chip device for studying the function of placental bile acid transporters according to claim 4, characterized in that: The pneumatic assembly comprises: Two second sleeves (15), both of which are fixed on the bottom of the platform (1); Two third pistons (21), the two third pistons (21) are respectively slidably disposed in two second sleeves (15), and each of the second sleeves (15) is fixed with a connecting block (22); Two three-way pipes (12), both of the three-way pipes (12) are connected to the second sleeve (15), and the other two ends of the two three-way pipes (12) are respectively connected to two adjacent first sleeves (11); A first connecting unit and a second connecting unit, wherein the first connecting unit and the second connecting unit are respectively connected to two connecting blocks (22).

6. A microfluidic chip device for studying the function of placental bile acid transporters according to claim 5, characterized in that: The power assembly comprises: A door frame (13), wherein the door frame (13) is fixed to the bottom of the platform (1); A plurality of motors (14), wherein the plurality of motors (14) are all fixed at the bottom of the portal frame (13); A plurality of crankshafts (20) are rotatably arranged at the bottom of the platform (1), and the plurality of crankshafts (20) and the output shafts of the plurality of motors (14) are fixed one to one.

7. A microfluidic chip device for studying the function of placental bile acid transporters according to claim 6, characterized in that: The first connecting unit includes: A connecting rod (23), wherein the connecting rod (23) is rotationally connected to one of the connecting blocks (22), and the connecting rod (23) is rotationally connected to the nearest crankshaft (20).

8. A microfluidic chip device for studying the function of placental bile acid transporters according to claim 7, characterized in that: The second connecting unit includes: A sleeve rod (28), wherein the sleeve rod (28) is rotatably connected to another connecting block (22); A fourth piston (27), wherein the fourth piston (27) is fixed to the end of the sleeve rod (28); A mounting block (24), a third sleeve (25) being fixed to a side wall of the mounting block (24), a fourth piston (27) being slidably disposed in the third sleeve (25), and the mounting block (24) being rotationally connected to a nearby crankshaft (20); A valve (26) is provided through the side wall of the third sleeve (25).

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