Rapid breeding method of ice hockey seedlings

Abstract

The invention belongs to the technical field of Ipomoea batatas seedling culture, and particularly relates to a rapid breeding method of Ipomoea batatas seedlings. The method comprises the following steps: seed collection: collecting completely mature ice hockey fruit pods, and ventilating and drying in a shade place; seed treatment: after the fruit pods are dried, selecting full fruit pods without mildewing and diseases and insect pests, and performing cold storage treatment; opening the refrigerated pods to obtain ice hockey seeds, and sterilizing and disinfecting the seeds; tissue culture inoculation and seedling culture: uniformly distributing the seeds on a culture medium in a tissue culture bottle, and culturing the seeds in a culture room to obtain tissue culture seedlings; and seedling hardening: culturing the cultured tissue culture seedlings under natural conditions. According to the breeding method, the emergence time can be shortened, and the emergence rate can be increased; one-time seedling formation is carried out through a specific tissue culture medium, the tissue culture time is shortened, and the link that tissue culture seedlings need to be transferred at present is omitted; through the specific tissue culture seedling hardening matrix, the seedling rate and the conversion rate of pseudobulb are improved, and the seedling hardening earnings are increased.

Description

Technical Field

[0001] The invention belongs to the technical field of ice ball seedling cultivation, and in particular relates to a rapid breeding method for ice ball seedlings. Background Art

[0002] Ice ball is a kind of Trillium medicinal material listed in the Pharmacopoeia of the People's Republic of China (Volume 1). Its origin is the perennial plants Pleione yunnanensis Rolfe and Pleione bulbocodioides (Franch.) Rolfe of the Orchidaceae family. Among them, Pleione bulbocodioides is the only origin plant of Trillium medicinal material in the formula granule standard. It has the effects of clearing away heat and detoxifying, resolving phlegm and dispersing nodules. It is mainly used for carbuncle, furuncle, scrofula, phlegm nodules, snake and insect bites, leukorrhea and lumps. Modern pharmacological studies have shown that it has a strong effect against breast cancer, esophageal cancer and gastric cancer.

[0003] At present, the main breeding methods of ice ball seedlings are sexual reproduction (seed reproduction) and asexual reproduction. Among them, asexual reproduction is mainly pseudobulb reproduction. Pseudobulb reproduction has problems such as seed source degradation, serious diseases and insect pests, and low yield. Sexual reproduction is mainly seed direct seeding and tissue culture. The current tissue culture technology is to transfer roots 2-3 months after seeding, and then take them out of the bottle 6-8 months after rooting. The cost of tissue culture seedlings is high and the tissue culture time is long. The growth is slow after hardening, the number of pseudobulb replacement seedlings is small, and the seedling success rate is low, which greatly limits the widespread promotion and application of ice ball seedlings. Ice ball seeds have the characteristics of orchid seeds, i.e., extremely small, no endosperm, symbiotic bacteria dependence, etc., the germination of its seeds has certain limitations, and the seed natural germination rate is less than 10%, and the general group culture sowing is about 2 months to emerge, and the seedling stage grows slowly, and it takes about 3-4 months from emergence to leaf growth. Therefore, the current main group culture direct seeding technology is to transfer and cultivate once when the leaves are not grown, and the group culture time is long, the cost is high, the survival rate is low, and the pseudobulb is slow to form. In addition, the existing seedling hardening technology is still immature, the seedling replacement rate is low, the hardening seedling pests are serious and the seedling success rate is low, and the pseudobulb needs more than 1 and a half years of hardening time to reach 1cm. Summary of the invention

[0004] The purpose of the present invention is to provide a rapid breeding method for ice ball seedlings, which can effectively improve the germination rate and seedling rate of seeds, shorten the tissue culture time, reduce the seedling breeding cost, realize standardized and factory-based production of ice ball seedlings, obtain a large number of high-quality ice ball seed seedlings, and meet the needs of ice ball seed planting.

[0005] The objective of the present invention is achieved through the following technical solutions:

[0006] A method for rapid propagation of ice ball seedlings, comprising the following steps:

[0007] S1. Seed collection: Collect fully mature ice ball fruit pods, dry them in a cool place with ventilation, and keep turning them in real time during the drying process;

[0008] S2. Seed treatment: After the fruit pods are dried, plump, mold-free, disease-free and insect-free fruit pods are selected and placed in a refrigerated storage at 0-5°C for more than 5 days; the refrigerated fruit pods are opened to obtain ice ball seeds, and the seeds are sterilized;

[0009] S3, tissue culture inoculation and seedling culture: the seeds are evenly distributed on the culture medium in the tissue culture bottle and placed in a culture room to be cultured into tissue culture seedlings;

[0010] S4. Hardening of seedlings: The cultured tissue culture seedlings are cultured in bottles and substrates to adapt to natural conditions.

[0011] Furthermore, the sterilization treatment includes disinfection with 75% alcohol for 30-50 seconds, disinfection with 0.2-0.4% mercuric chloride (i.e. mercuric chloride HgCl2) for 10-20 minutes, and washing with sterile water for 6-7 times.

[0012] Preferably, the sterilization treatment includes disinfection with 75% alcohol for 30 seconds, disinfection with 0.2% mercuric chloride for 12 minutes, and washing with sterile water for 6-7 times.

[0013] The culture medium uses MS as a basic culture medium and further comprises the following components: 1-1.5 mg / L NAA, 0.3-0.45 mg / L BA, 30-45 g / L potato juice, 0.5-1 g / L activated carbon, 30-40 g / L sucrose, and 5-8 g / L agar.

[0014] Preferably, the culture medium uses MS as the basic culture medium and also includes the following components: 1.5 mg / L NAA, 0.4 mg / L BA, 30 g / L potato juice, 0.5 g / L activated carbon, 30 g / L sucrose, and 5 g / L agar.

[0015] Among them, MS is the basic culture medium for plant tissue culture, which generally contains basic ingredients such as inorganic salts, sucrose, vitamins and water; NAA is naphthylacetic acid, which is a plant growth regulator; BA is 6-benzylpurine, which is a plant growth regulator.

[0016] In a specific embodiment, the amount of seeds distributed on the culture medium is 0.5-0.8 mg.

[0017] The conditions of the culture room are: temperature 25°C, light 10h / day, darkness 14h / day, light intensity 5000-6000lux.

[0018] Under the conditions of the culture room, the seedlings are hardened after 5-6 months of cultivation, including: 10-15 days after inoculation, the ice ball seeds begin to germinate, after 56-73 days, the pseudobulbs and fibrous roots of the seedlings are obviously formed, and after 63-77 days, when the diameter of the pseudobulbs of the tissue culture seedlings reaches more than 0.5mm and 2-3 fibrous roots grow, the tissue culture bottles can be moved out of the culture room for seedling hardening.

[0019] Furthermore, the bottle culture and matrix cultivation include: moving the tissue culture seedlings together with the tissue culture bottles containing the culture medium to room temperature or a greenhouse with a sunshade net to adapt to natural conditions for cultivation for 5-10 days; taking out the tissue culture seedlings and washing the culture medium, mixing the seeds with pests and diseases, and then transferring them to the matrix seedling bed for cultivation.

[0020] Preferably, the pest control seed dressing adopts a mixed agent of carbendazim, iprodione and thiram in a mass ratio of (0.5-1):(0.5-1):(0.5-1);

[0021] Furthermore, the transfer to the substrate seedling bed for cultivation is to transplant the tissue culture seedlings to a mixed substrate of peat soil and coconut bran, cover with peat soil with a thickness of 0.5-1.5 cm, and then cover with pine needles with a thickness of 3-4 cm, and maintain the humidity at 60-70%.

[0022] Furthermore, the mass ratio of the peat soil to the coconut husk is 10-14:1-2.

[0023] According to the method of the present invention, seedling hardening is carried out. 30 days after the tissue culture seedlings emerge, a balanced water-soluble foliar fertilizer is sprayed once, 50-80 ml / mu, pseudobulbs are replaced 3 months after the emergence, new seedlings are fully grown after 7 months, the average pseudobulb proliferation coefficient is 1.2-1.4, and the seedling hardening success rate is more than 140%.

[0024] According to the method of the present invention, the survival rate of the seedlings obtained by hardening the seedlings for field transplanting is above 95%.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] (1) Shorten the germination time and improve the germination rate: Through pre-sowing treatment of seeds, the germination time of tissue culture sowing is shortened from the current 2 months after sowing to about 15 days, and the germination rate is increased from 30-40% to more than 70%;

[0027] (2) Shortened tissue culture time and reduced seedling costs: The one-time seedling technology uses a specific tissue culture medium to allow roots to form seedlings once after sowing, thus reducing the number of transfers required for tissue culture seedlings. The tissue culture time is shortened from 8-9 months to 6 months, reducing the tissue culture cost of seedlings.

[0028] (3) Improved seedling survival rate: Through the use of a specific tissue culture seedling hardening matrix, the matrix has good water retention and air permeability and can reduce the occurrence of diseases and insect pests, thereby promoting the growth of seedling pseudobulbs, improving the seedling survival rate and pseudobulb conversion rate, and increasing the benefits of seedling hardening.

[0029] Other features and advantages of the present invention will be described in the following description, and partly become obvious from the description, or be understood by practicing the present invention. The purpose and other advantages of the present invention can be realized and obtained by the specific embodiments pointed out in the description. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 The tissue culture seedlings were cultured in the culture bottle for 30 days;

[0031] Figure 2 The tissue culture seedlings were cultured in the culture bottle for 60 days;

[0032] Figure 3 The tissue culture seedlings were cultured in the culture bottle for 120 days;

[0033] Figure 4 The tissue culture seedlings were cultured in the culture bottle for 180 days;

[0034] Figure 5 To harden the seedlings in the substrate seedbed of the seedling hardening shed;

[0035] Figure 6 To plant the seedlings after hardening them for 8 months;

[0036] Figure 7 This is a numerical distribution diagram of the diameter of the pseudobulb of the seedlings in Example 1. DETAILED DESCRIPTION

[0037] In order to make the purpose, technical scheme and advantages of the present invention clearer, the implementation scheme of the present application will be described in detail below in conjunction with specific examples. The technical terms and scientific terms used in the present invention have the meanings generally understood by those of ordinary skill in the art to which the present invention belongs, without other definitions. Without departing from the concept of the present invention, those skilled in the art may make various improvements and changes to the specific implementation methods of the present invention specification, which all belong to the protection scope of the present invention. The raw materials used in the present invention are all commercially available unless otherwise specified.

[0038] Example 1

[0039] A method for rapid propagation of ice ball seedlings, comprising the following steps:

[0040] S1. Seed collection: In September, according to the maturity of the ice ball fruit pods, fully mature ice ball fruit pods were collected and dried in a cool place with ventilation, and kept turning in real time during the drying process;

[0041] S2. Seed treatment: After the pods are dried, select the plump, mold-free, disease-free pods and place them in a refrigerator at 4°C for more than 5 days for later use;

[0042] Use scissors to cut an opening in the refrigerated fruit pod, shake the ice ball seeds into the tea bag, put the seeds of 3-4 fruit pods into each tea bag, seal the tea bag after filling; put the tea bag containing ice ball seeds into a sterile bottle and transfer it to the clean workbench, disinfect it with 75% alcohol for 30 seconds, 0.2% mercuric chloride for 12 minutes, and wash it with sterile water for 6 times to complete the sterilization and disinfection treatment.

[0043] S3, tissue culture inoculation and seedling culture: the seeds are evenly distributed on the culture medium in the tissue culture bottle and placed in a culture room to be cultured into tissue culture seedlings;

[0044] The amount of seeds distributed on the culture medium is 0.8 mg / bottle. The culture medium uses MS as a basic culture medium and also includes the following components: 1.5 mg / L NAA, 0.4 mg / L BA, 30 g / L potato juice, 0.5 g / L activated carbon, 30 g / L sucrose, and 5 g / L agar; wherein MS is a basic culture medium for plant tissue culture, generally containing basic ingredients such as inorganic salts, sucrose, vitamins and water; NAA is naphthylacetic acid, which is a plant growth regulator; BA is 6-benzylpurine, which is a plant growth regulator.

[0045] The tissue culture bottles were placed in a culture room for culture. The conditions in the culture room were: temperature 25°C, light 10h / day, darkness 14h / day, and light intensity 6000lux.

[0046] Under the conditions of the culture room, the ice ball seeds began to germinate 13 days after inoculation, and the tissue culture seedlings observed on the 30th day of culture were as follows: Figure 1 As shown in Figure 2, the tissue culture seedlings observed on the 60th day of culture were Figure 2 As shown in Figure 1, pseudobulbs and fibrous roots of the seedlings were clearly formed on the 103rd day of culture, and the tissue culture seedlings observed on the 120th day of culture were Figure 3 As shown in Figure 1, the tissue culture seedlings observed on the 180th day of cultivation were Figure 4 As shown, when the diameter of the pseudobulb of the tissue culture seedling reaches 0.5-2mm and 2-3 fibrous roots grow, the tissue culture bottle is moved out of the culture room and prepared for seedling hardening.

[0047] S4. Hardening of seedlings: The cultured tissue culture seedlings are cultured in bottles and substrates to adapt to natural conditions.

[0048] The tissue culture seedlings together with the tissue culture bottles containing the culture medium were moved to a seedling acclimatization shed with a sunshade net to adapt to natural conditions for 7 days; then the tissue culture seedlings were taken out and the culture medium was washed, and the seeds were mixed with a mixture of carbendazim, cyprodinil and thiram in a ratio of 1:1:1 for pest and disease prevention, and then transferred to the matrix seedling bed for cultivation. Specifically, the tissue culture seedlings were sown on a mixed matrix of peat soil and coconut bran in a mass ratio of 12:1, covered with 1 cm of peat soil, and then covered with 4 cm of pine needles, and the humidity was maintained at 70%. Figure 5 The seedling hardening shed is maintained at a temperature of 25-28° C. and is covered with a sunshade net having a light transmittance of 70%.

[0049] Seedlings began to emerge on the 40th day of hardening, and were basically completed on the 66th day. On the 70th day, the seedlings were sprayed with a balanced water-soluble foliar fertilizer at a dosage of 60 ml / mu. On the 110th day, the seedlings were dug up and the seedling rate and pseudobulb diameter were counted.

[0050] The pseudobulb diameters of 90 seedlings were tested, and the numerical distribution of pseudobulb diameters of seedlings is shown in the figure below: Figure 7 As shown, the diameters are mostly distributed in the range of 10-13 mm. According to statistics, the average diameter of the pseudobulbs of the tested seedlings is 1.12 cm, and the maximum is 1.52 cm. The average coefficient of pseudobulb proliferation is 1.45, and the seedling hardening rate is 145%.

[0051] S5. The seedlings obtained by hardening were transplanted to a field with a sunshade net. After 5 months, the survival rate in the field was found to be 96.4%.

[0052] Example 2

[0053] A method for rapid propagation of ice ball seedlings, comprising the following steps:

[0054] S1. Seed collection: In September, according to the maturity of the ice ball fruit pods, fully mature ice ball fruit pods were collected and dried in a cool place with ventilation, and kept turning in real time during the drying process;

[0055] S2. Seed treatment: After the pods are dried, select the plump, mold-free, disease-free pods and place them in a refrigerator at 2°C for more than 5 days for later use;

[0056] Use scissors to cut an opening in the refrigerated fruit pod, shake the ice ball seeds into the tea bag, put the seeds of 3-4 fruit pods into each tea bag, seal the tea bag after filling; put the tea bag containing ice ball seeds into a sterile bottle and transfer it to the clean workbench, disinfect it with 75% alcohol for 30 seconds, 0.2% mercuric chloride for 12 minutes, and wash it with sterile water for 6 times to complete the sterilization and disinfection treatment.

[0057] S3, tissue culture inoculation and seedling culture: the seeds are evenly distributed on the culture medium in the tissue culture bottle and placed in a culture room to be cultured into tissue culture seedlings;

[0058] The amount of seeds distributed on the culture medium is 0.5 mg / bottle, and the culture medium uses MS as the basic culture medium and also includes the following components: 1 mg / L NAA, 0.3 mg / L BA, 45 g / L potato juice, 1 g / L activated carbon, 40 g / L sucrose, and 8 g / L agar;

[0059] The tissue culture bottles were placed in a culture room for culture. The conditions in the culture room were: temperature 25°C, light 10h / day, darkness 14h / day, and light intensity 6000lux.

[0060] Under the conditions of the culture room, the ice ball seeds began to germinate 12 days after inoculation. The pseudobulbs and fibrous roots of the seedlings were obviously formed on the 100th day of culture. When the pseudobulbs of the tissue cultured seedlings reached a diameter of 0.5-2mm and 2-3 fibrous roots grew on the 177th day of culture, the tissue culture bottles were moved out of the culture room for seedling hardening.

[0061] S4. Hardening of seedlings: The cultured tissue culture seedlings are cultured in bottles and substrates to adapt to natural conditions.

[0062] The tissue culture seedlings together with the tissue culture bottles containing the culture medium were moved to a seedling acclimatization shed with a sunshade net to adapt to natural conditions for 7 days, after which the tissue culture seedlings were taken out and the culture medium was washed, and the seeds were mixed with a mixture of carbendazim, cyprodinil and thiram in a mass ratio of 1:1:1 for pest and disease prevention, and then transferred to the matrix seedling bed for cultivation. Specifically, the tissue culture seedlings were sown on a mixed matrix of peat soil and coconut bran in a mass ratio of 12:1, covered with 1 cm of peat soil, and then covered with 4 cm of pine needles, and the humidity was maintained at 70%. Figure 5 The seedling hardening shed is maintained at a temperature of 25-28° C. and is covered with a sunshade net having a light transmittance of 70%.

[0063] On the 35th day of hardening, the seedlings began to emerge and were basically completed on the 64th day. On the 70th day, the seedlings were sprayed with a balanced water-soluble foliar fertilizer at a dosage of 60 ml / mu. On the 110th day, the seedlings were dug up and the seedling rate and pseudobulb diameter were counted.

[0064] The diameter of pseudobulbs of 90 seedlings was tested and the average diameter was 1.19 cm and the maximum was 1.58 cm. The average pseudobulb proliferation coefficient was 1.42 and the seedling hardening rate was 142%.

[0065] S5. The mature seedlings obtained by hardening were transplanted to a field with a sunshade net. After 5 months, the survival rate in the field was found to be 97.5%.

[0066] Example 3

[0067] A method for rapid propagation of ice ball seedlings, comprising the following steps:

[0068] S1. Seed collection: In September, according to the maturity of the ice ball fruit pods, fully mature ice ball fruit pods were collected and dried in a cool place with ventilation, and kept turning in real time during the drying process;

[0069] S2. Seed treatment: After the pods are dried, select the plump, mold-free, disease-free pods and place them in a refrigerator at 4°C for more than 5 days for later use;

[0070] Use scissors to cut an opening in the refrigerated fruit pod, shake the ice ball seeds into the tea bag, put the seeds of 3-4 fruit pods into each tea bag, seal the tea bag after filling; put the tea bag containing ice ball seeds into a sterile bottle and transfer it to the clean workbench, disinfect it with 75% alcohol for 30 seconds, 0.2% mercuric chloride for 12 minutes, and wash it with sterile water for 6 times to complete the sterilization and disinfection treatment.

[0071] S3, tissue culture inoculation and seedling culture: the seeds are evenly distributed on the culture medium in the tissue culture bottle and placed in a culture room to be cultured into tissue culture seedlings;

[0072] The amount of seeds distributed on the culture medium is 0.8 mg / bottle, and the culture medium uses MS as the basic culture medium and also includes the following components: 1.5 mg / L NAA, 0.45 mg / L BA, 34 g / L potato juice, 1 g / L activated carbon, 30 g / L sucrose, and 5 g / L agar;

[0073] The tissue culture bottles were placed in a culture room for culture. The conditions in the culture room were: temperature 25°C, light 10h / day, darkness 14h / day, and light intensity 6000lux.

[0074] Under the conditions of the culture room, it was observed that the ice ball seeds began to germinate 14 days after inoculation. On the 105th day of culture, the pseudobulbs and fibrous roots of the seedlings were obviously formed. On the 181st day of culture, when the diameter of the pseudobulbs of the tissue cultured seedlings reached 0.5-2mm and 2-3 fibrous roots grew, the tissue culture bottles were moved out of the culture room and prepared for seedling hardening.

[0075] S4. Hardening of seedlings: The cultured tissue culture seedlings are cultured in bottles and substrates to adapt to natural conditions.

[0076] The tissue culture seedlings together with the tissue culture bottles containing the culture medium were moved to a seedling acclimatization shed with a sunshade net to adapt to natural conditions for 7 days; then the tissue culture seedlings were taken out and the culture medium was washed, and the seeds were mixed with a mixture of carbendazim, cyprodinil and thiram in a mass ratio of 1:1:1 for pest and disease prevention, and then transferred to the matrix seedling bed for cultivation. Specifically, the tissue culture seedlings were sown on a mixed matrix of peat soil and coconut bran in a mass ratio of 10:1, covered with 0.5 cm of peat soil, and then covered with 3 cm of pine needles, and the humidity was maintained at 60%. Figure 5 The seedling hardening shed is maintained at a temperature of 25-28° C. and is covered with a sunshade net having a light transmittance of 70%.

[0077] On the 37th day of hardening, the seedlings began to emerge and were basically completed on the 67th day. On the 70th day, the seedlings were sprayed with a balanced water-soluble foliar fertilizer at a dosage of 80 ml / mu. On the 110th day, the seedlings were dug up and the seedling rate and pseudobulb diameter were counted.

[0078] The diameter of pseudobulbs of 90 seedlings was tested and the average diameter was 1.09 cm and the maximum was 1.48 cm. The average coefficient of pseudobulb proliferation was 1.41 and the seedling hardening rate was 141%.

[0079] S5. The mature seedlings obtained by hardening were transplanted to a field with a sunshade net. After 5 months, the survival rate in the field was found to be 98.1%.

[0080] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for rapid propagation of ice ball seedlings, characterized in that: The following steps are involved: Seed collection: Collect fully mature ice ball fruit pods and dry them in a cool and ventilated place; Seed treatment: After the pods are dried, select the plump, mildew-free, disease-free and insect-free pods for cold storage; open the pods after cold storage to obtain the ice ball seeds, and sterilize the seeds; Tissue culture inoculation and seedling culture: The seeds are evenly distributed on the culture medium in the tissue culture bottle and placed in the culture room to culture into tissue culture seedlings; the conditions in the culture room are: temperature 25°C, light 10h / day, dark 14h / day, light intensity 5000-6000lux; Seedling hardening: The cultured tissue culture seedlings are cultured in bottles and substrates to adapt to natural conditions.

2. The rapid propagation method of ice ball seedlings according to claim 1, characterized in that: The culture medium uses MS as a basic culture medium and further comprises the following components: 1-1.5 mg / L NAA, 0.3-0.45 mg / L BA, 30-45 g / L potato juice, 0.5-1 g / L activated carbon, 30-40 g / L sucrose, and 5-8 g / L agar.

3. The method for rapid propagation of ice ball seedlings according to claim 2, characterized in that: The culture medium uses MS as a basic culture medium and further comprises the following components: 1.5 mg / L NAA, 0.4 mg / L BA, 30 g / L potato juice, 0.5 g / L activated carbon, 30 g / L sucrose, and 5 g / L agar.

4. The method for rapid propagation of ice ball seedlings according to claim 1, characterized in that: The sterilization treatment includes sterilization with 75% alcohol for 30-50 seconds, sterilization with 0.2-0.4% mercuric chloride for 10-20 minutes, and washing with sterile water for 6-7 times.

5. The method for rapid propagation of ice ball seedlings according to claim 1, characterized in that: The amount of seeds distributed on the culture medium is 0.5-0.8 mg.

6. The method for rapid propagation of ice ball seedlings according to claim 1, characterized in that: The bottle culture and substrate cultivation include: moving the tissue culture seedlings together with the tissue culture bottles containing the culture medium to room temperature or a greenhouse with a sunshade net to adapt to natural conditions for 5-10 days; taking out the tissue culture seedlings and washing the culture medium, mixing the seeds with pests and diseases, and then transferring them to the substrate seedling bed for cultivation.

7. The method for rapid propagation of ice ball seedlings according to claim 6, characterized in that: The pest control seed dressing adopts a mixed agent of carbendazim, iprodione and thiram in a mass ratio of (0.5-1):(0.5-1):(0.5-1).

8. The method for rapid propagation of ice ball seedlings according to claim 6, characterized in that: The transfer to the matrix seedling bed for cultivation is to transplant the tissue culture seedlings onto a mixed matrix of peat soil and coconut bran, cover the peat soil with a thickness of 0.5-1.5 cm, and then cover with pine needles with a thickness of 3-4 cm, and maintain the humidity at 60-70%.

9. The method for rapid propagation of ice ball seedlings according to claim 8, characterized in that: The mass ratio of the peat soil to the coconut bran is 10-14:1-2.

10. The method for rapid propagation of ice ball seedlings according to claim 1, characterized in that: The cold storage treatment is a cold storage treatment at 0-5°C for more than 5 days.

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