Device and method for increasing leech hirudin content based on natural secretion method

Abstract

The invention relates to the field of leech breeding, and particularly discloses a device and method for increasing the hirudin content of leeches based on a natural secretion method. According to the leech feeding device, the feeding part, the anti-blocking assembly and the floating ball are arranged to form the leech feeding device, the feeding slurry is contained in the loading pipe, then the loading pipe is wrapped with the casing to avoid leakage of the feeding slurry, and after the loading pipe and the floating ball are thrown into a leech culture pond, the floating ball can float on the water surface; the loading pipe is vertically suspended in the water tank through the connecting line and the connecting buckle, the leeches can be adsorbed on the casing to suck the feeding slurry in the loading pipe, animal skin tissues are simulated through the casing, the leeches are induced to secrete hirudin in the sucking process, and then the hirudin secreting capacity of the bred leeches is exercised and improved; and the anti-blocking assembly can prevent the solid part in the feeding slurry from blocking the leaking holes, and it is guaranteed that the leeches can smoothly suck the feeding slurry in the loading pipe.

Description

Technical Field

[0001] The invention belongs to the technical field of leech breeding, and particularly relates to a device and method for increasing the hirudin content of leeches based on a natural secretion method. Background Art

[0002] Hirudin is a substance secreted by leeches that has anticoagulant and decoagulant effects. It is considered to be the strongest biological anticoagulant substance today and has excellent clinical application prospects.

[0003] There are two ways to obtain hirudin: leech extraction and chemical recombination. Compared with natural hirudin, the hirudin formed by chemical recombination is not as effective, has lower activity, and has greater adverse reactions, which cannot meet the market demand. The leech extraction method requires obtaining it from the leech. When wild leeches lie on the surface of animals to suck, in order to prevent blood clotting in the animal's wound, they will secrete hirudin to hinder blood clotting. Therefore, wild leeches contain more hirudin. Currently, the source of wild leeches is getting less and less, and the price is getting more and more expensive. Most of the leeches on the market are farmed leeches, and artificially raised leeches are more and more expensive. The amount of hirudin in the bodies of farmed leeches is relatively small, because farmed leeches are mostly fed with feed and other substances, and do not need to secrete more hirudin to prevent blood coagulation. As a result, the amount of hirudin that can be extracted from the same weight of farmed leeches is less than that from wild leeches. In addition, farmed leeches are difficult to breed and have a low yield, which restricts the large-scale application of hirudin. In order to increase the yield of hirudin, it is necessary to stimulate artificially fed leeches to secrete more hirudin, thereby increasing the content of hirudin that can be extracted per unit weight of farmed leeches. Summary of the invention

[0004] In order to overcome the deficiencies of the prior art, the purpose of the present invention is to provide a device and method for increasing the hirudin content of leeches based on the natural secretion method, so as to solve the problem of low hirudin content in artificially cultivated leeches.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] The device for increasing the content of leech hirudin based on the natural secretion method includes:

[0007] A feeding part, used for immersing in a breeding pond to feed leeches, comprises a loading tube for loading feeding slurry, a leakage hole provided on the loading tube and a tube cover threadedly connected to the end of the loading tube, wherein the loading tube and the tube cover are externally covered with casings for preventing leakage of feeding slurry;

[0008] An anti-blocking component, arranged on the tube cover, is used to prevent solids in the feeding slurry from blocking the leakage hole;

[0009] The float is connected to the pipe cover via a connecting line.

[0010] Preferably, the end of the tube cover is fixedly connected to a terminal, and the open end of the casing is sleeved on the terminal and tied and sealed.

[0011] Preferably, the casing is made of pig small intestine or artificial casing.

[0012] Preferably, the feeding slurry comprises:

[0013] Pig, bovine, chicken or duck blood, plasma or serum;

[0014] An induction solution prepared by mixing sodium chloride and arginine;

[0015] Extracts from the meat and viscera of pigs, cattle, chickens or ducks.

[0016] Preferably, the anti-blocking assembly includes a rotating rod rotatably connected to the tube cover, a lever fixedly mounted on the rotating rod, and a connecting rod fixedly mounted on the lever, and a spring is provided inside the tube cover, with both ends respectively connected to the rotating rod and the tube cover.

[0017] Preferably, the lever is provided with a front surface abutting against the inner wall of the loading tube.

[0018] Preferably, a connecting ring is fixedly connected to the end head, and the end of the connecting line is connected to a connecting buckle that is clamped on the connecting ring.

[0019] A method for increasing the content of leech hirudin based on the natural secretion method, and a device for increasing the content of leech hirudin based on the natural secretion method, comprising:

[0020] After the casing is put on the loading tube, the feed slurry prepared according to the proportion is poured into the loading tube, the spring is rotated by the rotating rod to store energy, the tube cover is installed on the loading tube, the end of the casing is put on the end head and tied tightly, and the connecting buckle is fastened to connect the float ball;

[0021] Throw the loading tube with feeding slurry into the breeding pond, the loading tube sinks into the pond, the float floats on the water surface, so that the loading tube stands upright in the water for leeches to suck, the energy-storing spring drives the lever to rotate through the rotating rod to ensure that the leakage hole is unobstructed and the leech falls off the casing after sucking enough, and feeds for 2 to 5 days;

[0022] The leeches are taken out and rinsed, and then crushed, precipitated, centrifuged to obtain the centrifuge liquid, filtered to obtain the liquid, and dried to obtain a high-purity hirudin extract.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] The present invention forms a leech feeding device by arranging a feeding part, an anti-blocking component and a floating ball, wherein feeding slurry is loaded inside a loading tube, and then the loading tube is wrapped with an intestinal casing to prevent leakage of the feeding slurry. After the loading tube and the floating ball are thrown into a leech breeding pond, the floating ball floats on the water surface, and the loading tube is vertically suspended in the water pond through a connecting line and a connecting buckle, and the leech can be adsorbed on the intestinal casing to suck the feeding slurry inside the loading tube, and the intestinal casing is used to imitate animal skin tissue to induce the leech to secrete hirudin during the sucking process, thereby exercising and improving the hirudin secretion ability of the cultured leech, and improving the hirudin content of the cultured leech, and the anti-blocking component can prevent the solid part in the feeding slurry from blocking the leakage hole, thereby ensuring that the leech can smoothly suck the feeding slurry in the loading tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0026] Figure 2 It is a schematic diagram of the overall cross-sectional structure of the present invention;

[0027] Figure 3 It is a schematic diagram of the explosion structure of the feeding part of the present invention;

[0028] Figure 4 It is a schematic diagram of the tube cover structure of the present invention;

[0029] Figure 5 For the present invention Figure 4 A magnified image of the middle part;

[0030] Figure 6 It is a schematic diagram of the cross-sectional structure of the tube cover of the present invention;

[0031] In the figure: 1. loading tube; 2. leakage hole; 3. tube cover; 4. casing; 5. end; 6. connecting buckle; 7. connecting line; 8. float; 9. rotating rod; 10. lever; 11. spring; 12. connecting rod. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] Embodiment 1:

[0034] See also Figure 1 - Figure 6 As shown, the device for increasing the content of leech hirudin based on the natural secretion method is characterized by comprising:

[0035] The feeding part is used to immerse in the breeding pond to feed leeches, and includes a loading tube 1 for loading feeding slurry, a leakage hole 2 provided on the loading tube 1, and a tube cover 3 threadedly connected to the end of the loading tube 1. The loading tube 1 and the tube cover 3 are externally covered with a casing 4 for preventing leakage of the feeding slurry;

[0036] An anti-blocking component, arranged on the tube cover 3, is used to prevent solids in the feeding slurry from blocking the leakage hole 2;

[0037] The float ball 8 is connected to the pipe cover 3 via a connecting line 7 .

[0038] A leech feeding device is formed by providing a feeding part, an anti-blocking component and a float 8, the feeding slurry is loaded inside the loading tube 1, and then the loading tube 1 is wrapped with a casing 4 to prevent the feeding slurry from leaking out. After the loading tube 1 and the float 8 are thrown into the leech breeding pond, the float 8 will float on the water surface, and the loading tube 1 is vertically suspended in the water pond through the connecting line 7 and the connecting buckle 6. The leech can be adsorbed on the casing 4 to suck the feeding slurry inside the loading tube 1, and the casing 4 is used to imitate the animal skin tissue to induce the leech to secrete hirudin during the sucking process, thereby exercising and improving the hirudin secretion ability of the cultured leech and increasing the hirudin content of the cultured leech. The anti-blocking component can prevent the solid part in the feeding slurry from blocking the leakage hole 2, thereby ensuring that the leech can smoothly suck the feeding slurry in the loading tube 1.

[0039] See also Figure 1 - Figure 3 As shown, the end of the tube cover 3 is fixedly connected with an end cap 5, and the open end of the casing 4 is sleeved on the end cap 5 and tied and sealed, the two ends of the casing 4 are respectively tied at the end of the loading tube 1 and the end of the tube cover 3, the end cap 5 is arranged at the end of the tube cover 3, and other components are arranged on the end cap 5. The casing 4 is tied to the end cap 5, which can effectively improve the sealing performance of the casing 4 and prevent the feeding slurry inside the casing 4 from leaking out.

[0040] The casing 4 is made of pig intestine or artificial casing, and the user can select it according to the needs to ensure that the leech mouthparts can break the casing 4 to suck the feeding pulp.

[0041] The feeding slurry comprises:

[0042] Blood, plasma or serum from pigs, cows, chickens or ducks. Try to choose fresh blood from livestock;

[0043] The induction solution prepared by mixing sodium chloride and arginine can be prepared by adding 300g of arginine and 800g of porcine plasma to 10L of 0.9% sodium chloride solution, and mixing them to prepare the induction solution;

[0044] The extract of meat and viscera of pigs, cows, chickens or ducks is made by blending the meat and viscera of livestock into a slurry and adding a portion of the induction liquid to complete the preparation.

[0045] See also Figure 4 - Figure 6 As shown, the anti-blocking assembly includes a rotating rod 9 rotatably connected to the tube cover 3, a lever 10 fixedly mounted on the rotating rod 9 and a connecting rod 12 fixedly mounted on the lever 10, and a spring 11 with two ends respectively connected to the rotating rod 9 and the tube cover 3 is provided inside the tube cover 3.

[0046] The user can rotate the rotating rod 9 to store energy for the clockwork 11. The energy-stored clockwork 11 will drive the lever 10 and the connecting rod 12 to rotate through the rotating rod 9. The lever 10 will rotate near the inner wall of the loading tube 1. When the feeding slurry poured into the loading tube 1 is a solid-liquid mixture, the lever 10 can push away the solid matter blocking the leakage hole 2, keeping the leakage hole 2 unobstructed, so that the juice in the feeding slurry can leak out of the leakage hole 2 for the leech to suck.

[0047] The lever 10 is provided with a front surface that abuts against the inner wall of the loading tube 1 , so that the lever 10 can easily push away the solid matter that is blocked in the leakage hole 2 .

[0048] A connecting ring is fixedly connected to the end 5, and the end of the connecting line 7 is connected to a connecting buckle 6 that is clamped on the connecting ring. The float 8 is connected to the end 5 through the connecting line 7 and the connecting buckle 6. The user can adjust the number of floats 8 connected to the end 5 according to the feeding amount, thereby adjusting the position of the loading tube 1 so that the loading tube 1 is suspended at different heights in the breeding pond for leeches to feed.

[0049] Method for increasing the content of leech hirudin based on natural secretion method. Device for increasing the content of leech hirudin based on natural secretion method, including:

[0050] After the casing 4 is put on the loading tube 1, the feed slurry prepared according to the proportion is poured into the loading tube 1, the spring 11 is rotated by the rotating rod 9 to store energy, the tube cover 3 is installed on the loading tube 1, the end of the casing 4 is put on the end 5 and tied tightly, and the connecting buckle 6 is fastened to connect the floating ball 8;

[0051] Throw the loading tube 1 with feeding slurry into the breeding pond, the loading tube 1 sinks into the pond, the float 8 floats on the water surface, so that the loading tube 1 stands upright in the water for the leech to suck, the energy-storing spring 11 drives the lever 10 to rotate through the rotating rod 9 to ensure that the leakage hole 2 is unobstructed and the leech falls off the casing 4 after sucking enough, and feeds for 2 to 5 days;

[0052] The leeches are taken out and rinsed, and then crushed, precipitated, centrifuged to obtain the centrifuge liquid, filtered to obtain the liquid, and dried to obtain a high-purity hirudin extract.

[0053] Embodiment 2:

[0054] The technical features of this embodiment that are different from those of the first embodiment are as follows:

[0055] In some shallow water leech breeding ponds, the float 8 and the connecting line 7 can be removed, and the feeding part can be directly poured and placed in the leech breeding pond for the leeches to suck. After the feeding is completed, the feeding part can be taken away and the breeding pond can be flushed with boiling water.

[0056] The standard parts used in the present invention can all be purchased from the market, and the special-shaped parts can be customized according to the description and the drawings. The specific connection methods of each part adopt conventional means such as mature bolts, rivets, welding, etc. in the prior art. The machinery, parts and equipment all adopt conventional models in the prior art, and the circuit connection adopts the conventional connection method in the prior art, which will not be described in detail here. The contents not described in detail in this specification belong to the prior art known to professional and technical personnel in this field.

[0057] In the description of the present invention, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. "Multiple" means two or more, unless otherwise clearly and specifically defined.

[0058] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0059] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0060] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example" or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, unless they are contradictory.

[0061] In the drawings of the embodiments disclosed in the present invention, only the structures related to the embodiments disclosed in the present invention are involved, and other structures can refer to the general design. In the absence of conflict, the same embodiment and different embodiments of the present invention can be combined with each other.

Claims

1. A device for increasing the content of leech hirudin based on the natural secretion method, characterized in that: include: A feeding part, used for being immersed in a breeding pond to feed leeches, comprises a loading tube (1) for loading feeding slurry, a leakage hole (2) provided on the loading tube (1) and a tube cover (3) threadedly connected to the end of the loading tube (1), wherein the loading tube (1) and the tube cover (3) are externally covered with a casing (4) for preventing leakage of the feeding slurry; an anti-blocking component, arranged on the tube cover (3) and used to prevent solid matter in the feeding slurry from blocking the leakage hole (2); The float ball (8) is connected to the pipe cover (3) via a connecting line (7).

2. The device for increasing the content of leech hirudin based on the natural secretion method according to claim 1, characterized in that: The end of the tube cover (3) is fixedly connected to a terminal head (5), and the open end of the casing (4) is sleeved on the terminal head (5) and tied and sealed.

3. The device for increasing the content of leech hirudin based on the natural secretion method according to claim 1, characterized in that: The casing (4) is made of pig small intestine or artificial casing.

4. The device for increasing the content of leech hirudin based on the natural secretion method according to claim 1, characterized in that: The feeding slurry comprises: Pig, bovine, chicken or duck blood, plasma or serum; An induction solution prepared by mixing sodium chloride and arginine; Extracts from the meat and viscera of pigs, cattle, chickens or ducks.

5. The device for increasing the content of leech hirudin based on the natural secretion method according to claim 1, characterized in that: The anti-blocking component comprises a rotating rod (9) rotatably connected to the pipe cover (3), a lever (10) fixedly mounted on the rotating rod (9), and a connecting rod (12) fixedly mounted on the lever (10); a spring (11) is arranged inside the pipe cover (3) with two ends respectively connected to the rotating rod (9) and the pipe cover (3).

6. The device for increasing the content of leech hirudin based on the natural secretion method according to claim 5, characterized in that: The lever (10) is provided with a front surface which abuts against the inner wall of the loading tube (1).

7. The device for increasing the content of leech hirudin based on the natural secretion method according to claim 2, characterized in that: A connecting ring is fixedly connected to the end head (5), and a connecting buckle (6) clamped on the connecting ring is connected to the end of the connecting line (7).

8. A method for increasing the content of leech hirudin based on natural secretion, characterized in that: The device for increasing the hirudin content of leeches based on the natural secretion method as described in any one of claims 1 to 7 comprises: After the casing (4) is put on the loading tube (1), the feed slurry prepared according to the proportion is poured into the loading tube (1), the spring (11) is rotated by the rotating rod (9) to store energy, the tube cover (3) is installed on the loading tube (1), the end of the casing (4) is put on the end head (5) and tied tightly, and the connecting buckle (6) is fastened to connect the floating ball (8); Throwing the loading tube (1) with the feeding slurry into the breeding pond, the loading tube (1) sinks into the pond, and the float (8) floats on the water surface, so that the loading tube (1) stands upright in the water for the leeches to suck food, and the energy-storing spring (11) drives the lever (10) to rotate through the rotating rod (9), ensuring that the leakage hole (2) is unobstructed and leaks liquid, and the leeches fall off the casing (4) after being full of food, and feed for 2 to 5 days; The leeches are taken out and rinsed, and then crushed, precipitated, centrifuged to obtain the centrifuge liquid, filtered to obtain the liquid, and dried to obtain a high-purity hirudin extract.

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