Solid beverage for preventing and treating atherosclerosis and preparation method thereof

Abstract

The invention relates to the technical field of functional health-care products, and discloses a solid beverage for preventing and treating atherosclerosis and a preparation method thereof. The solid beverage for preventing and treating atherosclerosis is prepared from the following raw material components in parts by mass: 40-60 parts of dendrobium officinale, 30-50 parts of astragalus membranaceus, 6-10 parts of angelica sinensis, 10-15 parts of small yellow ginger, 10-15 parts of one-clove garlic, 10-20 parts of euphausia superba oil, 3-6 parts of chitosan, 5-10 parts of attapulgite and 3-6 parts of stevia rebaudiana. The solid beverage for preventing and treating atherosclerosis, which is prepared according to the formula disclosed by the invention, can realize multi-way mitochondrial function repair so as to prevent and The preparation process can effectively retain the volatile oil, and can realize solidification of liquid components without additional absorbent.

Description

Technical Field

[0001] The invention relates to the technical field of functional health products, and in particular to a solid beverage for preventing and treating atherosclerosis and a preparation method thereof. Background Art

[0002] The high prevalence, high disability rate and high mortality rate of cardiovascular and cerebrovascular diseases make them a global public health issue that cannot be ignored at present. Atherosclerosis is the common pathological basis and main cause of ischemic cardiovascular and cerebrovascular diseases, which can then induce sudden outbreaks of cardiovascular and cerebrovascular diseases such as angina pectoris, myocardial infarction, heart failure, cerebral infarction, etc., causing disability and death. Therefore, based on the occurrence and development mechanism of atherosclerosis, the development of functional health products with significant preventive and therapeutic effects on atherosclerosis is not only an important topic to reduce the incidence of atherosclerosis and secondary cardiovascular and cerebrovascular diseases, but also can meet the public's real needs for self-conditioning in life to cope with atherosclerosis. At the same time, it can also alleviate the imbalance of supply and demand in the nutritional health market for the prevention and treatment of atherosclerosis caused by objective factors such as population aging, which is of great significance for protecting public health and reducing the family and social medical and economic burden induced by atherosclerosis and its complications.

[0003] In recent years, with the increasing prevalence of atherosclerosis and its secondary diseases, the public's awareness of the harmfulness of atherosclerosis has gradually increased, and residents' demand for functional health products for atherosclerosis has increased year by year. However, chemical drugs represented by statins cannot be used in functional health products due to adverse reactions such as liver damage and rhabdomyolysis. Therefore, highly efficient and non-toxic food ingredients and Chinese medicinal materials have become the first choice for the development of functional health products for the prevention and treatment of atherosclerosis. For health products for the prevention and treatment of atherosclerosis that require long-term use, functional health products for the prevention and treatment of atherosclerosis derived from highly efficient and non-toxic food ingredients or Chinese medicinal materials can simultaneously meet the public's safety needs for significant efficacy in the prevention and treatment of atherosclerosis and long-term use without toxic side effects. It has become the main direction of the development of functional health products for the prevention and treatment of atherosclerosis, and the market prospects are broad.

[0004] A large number of studies have shown that mitochondria are not only the "ATP factory" of cells, but also the key site for regulating cellular oxidative stress and apoptosis, and they maintain close contact with the adjacent organelle endoplasmic reticulum. When mitochondrial dysfunction occurs, it can affect the occurrence and development of atherosclerosis by aggravating the body's pathological processes such as oxidative stress, inflammatory response, cell apoptosis and cholesterol accumulation. At the same time, more and more studies have shown that mitochondrial dysfunction is not only similar to the TCM pathogenic theories of "liver, spleen and kidney deficiency, heat, phlegm and blood stasis" in the process of atherosclerosis, but also that the endosymbiotic theory believes that mitochondria originate from bacteria, so mitochondrial dysfunction is also closely related to intestinal flora imbalance in the process of atherosclerosis. Therefore, mitochondrial dysfunction runs through the explanation of the occurrence and development mechanism of atherosclerosis in traditional Chinese and Western medicine theories, and is the common mechanism of multiple pathogenic factors of atherosclerosis in traditional Chinese and Western medicine theories. Repairing the damaged mitochondrial function inside arterial vascular cells related to atherosclerosis is one of the main strategies for preventing and treating atherosclerosis. However, there is currently a lack of research reports and product development on functional health products specifically aimed at improving and repairing mitochondrial dysfunction to prevent and treat the occurrence and development of atherosclerosis. Therefore, the development of functional health products for the prevention and treatment of atherosclerosis urgently requires the introduction of a product that prevents and treats the occurrence and development of atherosclerosis based on repairing mitochondrial dysfunction, so as to improve the effectiveness of the prevention and treatment of atherosclerosis.

[0005] At the same time, most of the current preparations for the prevention and treatment of atherosclerosis are traditional drug dosage forms such as capsules, powders, and oral liquids. These product presentation methods often lead to consumers' fear of the concept of "drugs" and reduce compliance with use. Although Li Xinhui et al.'s patent "A health wine for the prevention and treatment of atherosclerosis and its preparation method" (ZL201610090817.1) has developed its preparation into a wine preparation by turning it into a daily food, its use of wine as a carrier cannot meet the needs of people who are inconvenient or cannot drink. Therefore, the current market for functional health products for the prevention and treatment of atherosclerosis urgently needs dosage products that have both the characteristics of daily food and the advantages of people without contraindications. Summary of the invention

[0006] Purpose of the invention: In view of the problems existing in the prior art, the purpose of the present invention is to provide a solid beverage for preventing and treating atherosclerosis. The solid beverage for preventing and treating atherosclerosis can not only prevent and treat atherosclerosis by repairing mitochondrial function in multiple ways, but also can realize the daily food form of the product dosage form and improve the compliance of users. In addition, when the product is used, it can be taken by dispersing it in water, with water as the carrier for taking, and there are no contraindications for the group of people. Another purpose of the present invention is to provide a method for preparing a solid beverage for preventing and treating atherosclerosis, which "can not only effectively retain volatile active ingredients such as volatile oils of small yellow ginger and single-headed garlic, but also can realize the solidification of liquid components such as Antarctic krill oil without the need to add additional absorbents."

[0007] Technical solution: A solid beverage for preventing and treating atherosclerosis is prepared from the following raw material components in parts by mass: 40-60 parts of Dendrobium officinale, 30-50 parts of Astragalus, 6-10 parts of Angelica sinensis, 10-15 parts of Turmeric, 10-15 parts of Garlic, 10-20 parts of Antarctic krill oil, 3-6 parts of chitosan, 5-10 parts of Attapulgite, and 3-6 parts of Stevia.

[0008] Furthermore, the Dendrobium officinale is a three-year-old medicinal-grade Dendrobium officinale powder that is crushed and sieved through an 80-120 mesh sieve.

[0009] Furthermore, the astragalus is astragalus powder obtained by crushing the dried roots of the leguminous plants Astragalus mongolica or Astragalus membranaceus and passing through a 60-mesh sieve.

[0010] Furthermore, the angelica is angelica powder obtained by crushing the dried roots of the umbelliferae plant angelica and passing through a 60-mesh sieve.

[0011] Furthermore, the small yellow turmeric is a fresh tuber of the small yellow turmeric plant of the ginger family.

[0012] Furthermore, the single-headed garlic is a dried bulb of the single-headed garlic of the Liliaceae family.

[0013] Furthermore, the chitosan is a pharmaceutical grade chitosan powder having a molecular weight of 50-90 kDa and passing through a 80-120 mesh sieve.

[0014] Furthermore, the attapulgite is a pharmaceutical adsorption grade attapulgite powder that has passed through a 80-120 mesh sieve.

[0015] Furthermore, the solid beverage has a total saponin content of ≥100 μg / g, a ferulic acid content of ≥20 μg / g, a total flavonoid content of ≥30 mg / g, a total polysaccharide content of ≥250 mg / g, and a volatile oil content of ≥10 mg / g. After being dispersed in water, the sedimentation ratio is ≥0.92 within 3 hours.

[0016] On the other hand, the present invention provides a method for preparing a solid beverage for preventing and treating atherosclerosis, comprising the following steps: according to any one of the above-mentioned solid beverages for preventing and treating atherosclerosis, the raw materials are: (1) After the astragalus and angelica are crushed to pass through a 60-mesh sieve, the astragalus and angelica powders are weighed, fully mixed and placed in an extraction device, deionized water is added, soaked, refluxed and decocted under normal pressure, and filtered; the filter residue is decocted again under the same conditions and filtered; the two filtrates are combined and concentrated under reduced pressure to a relative density of 0.4 to 0.8 under the conditions of a vacuum degree of 0.090 to 0.098 MPa and a temperature of 60 to 80 degrees Celsius to obtain a mixed extract of astragalus and angelica; (2) Wash and drain the turmeric, peel it and cut it into dices; (3) Peel the garlic cloves, remove impurities, wash and drain; (4) placing the pre-treated raw materials of turmeric and garlic obtained in steps (2) and (3) in a homogenizer, adding the mixed extract of astragalus and angelica obtained in step (1), and fully homogenizing with the homogenizer until the slurry passes through a 80-120 mesh sieve; (5) spraying the Antarctic krill oil evenly on the attapulgite powder, mixing thoroughly, and sealing and placing for 6 to 9 hours to obtain a mixture of the Antarctic krill oil and the attapulgite; (6) adding chitosan powder to the homogenate obtained in step (4), stirring and suspending the chitosan powder, and then evenly adding the chitosan powder to the mixture of Antarctic krill oil and attapulgite obtained in step (5) by passing the mixture through a 80-120 mesh sieve, and stirring and mixing the mixture evenly; (7) Grinding Dendrobium officinale and Stevia rebaudiana respectively until they pass through a 80-120 mesh sieve, and mixing the two uniformly by an equal amount increasing method to obtain a mixed powder of Dendrobium officinale and Stevia rebaudiana; (8) The mixture obtained in step (6) is added to the mixed powder of Dendrobium officinale and Stevia in equal amounts, and after being fully mixed, the mixture is passed through a 80-120 mesh sieve to obtain a solid beverage for preventing and treating atherosclerosis.

[0017] The formula principle of the present invention: The raw materials of the solid beverage for preventing and treating atherosclerosis provided by the present invention include dendrobium officinale, astragalus, angelica, turmeric, garlic, Antarctic krill oil, chitosan, attapulgite and stevia.

[0018] Among them, Dendrobium candidum is the dried stem of the orchid Dendrobium candidum. It tastes sweet and is slightly cold in nature; it enters the stomach and kidney meridians; it has the effects of nourishing yin and strengthening the spleen, benefiting the stomach and producing body fluid. There are many records of Dendrobium candidum regulating the stomach and intestines in the classic works of traditional Chinese medicine in the past dynasties. For example, "Shennong's Herbal Classic" records that Dendrobium candidum "thickens the stomach and intestines for a long time, lightens the body and prolongs life". "Compendium of Materia Medica" calls Dendrobium candidum "nourishes the stomach and benefits the body fluid, and is the supreme wonderful product". Current medicinal chemistry research has found that Dendrobium candidum is rich in polysaccharides, dendrobium alkaloids, phenanthrene compounds and various amino acids. In the present invention, the polysaccharides contained in Dendrobium candidum are mainly used to regulate intestinal flora disorders and inhibit the generation of enterogenic toxic metabolites that damage mitochondrial function, and strengthen the intestinal barrier to reduce the absorption of enterogenic toxic metabolites that damage mitochondrial function. This function corresponds to the "thick stomach and intestines" function in theory. At the same time, the viscosity of the aqueous solution given by the high content of polysaccharides in Dendrobium candidum is used to increase the stability of the product's dispersion in water. In addition, the significant antioxidant and lipid-lowering effects of dendrobium alkaloids and phenanthrene compounds are used to inhibit the generation of reactive oxygen species and reduce triglycerides, eliminating the high-fat and high-oxygen free radical microenvironment that damages mitochondria.

[0019] Astragalus is the dried root of Astragalus mongolica or Astragalus membranaceus, a plant of the leguminous family. It is sweet and warm in nature, and has the effects of "tonifying qi and raising yang, strengthening the body's defense system, promoting fluid production and nourishing blood". Current medicinal chemistry research reveals that Astragalus is rich in saponins, polysaccharides, flavonoids, amino acids, linolenic acid, linoleic acid and various trace elements. Modern pharmacological research has found that Astragalus has the effects of improving circulatory perfusion, enhancing myocardial contractility, scavenging free radicals and improving superoxide dismutase. In the present invention, the polysaccharides contained in Astragalus are mainly used to regulate intestinal flora disorders and inhibit the generation of intestinal toxic metabolites that damage mitochondrial function, and to strengthen the intestinal barrier and reduce the absorption of intestinal toxic metabolites that damage mitochondrial function; at the same time, the saponins contained in it, especially astragaloside IV, are used to improve the efficiency of mitochondrial oxidative phosphorylation and promote mitochondrial ATP synthesis to repair mitochondrial function, enhance myocardial contractility, improve circulation and prevent the formation of atherosclerosis. This effect corresponds to the theory of Chinese medicine for tonifying qi and raising yang in Astragalus. In addition, astragalus total flavonoids and its isoflavones are used to inhibit the generation of reactive oxygen species and lipid deposition, thereby eliminating the high-fat and high-oxygen free radical microenvironment that damages mitochondria during atherosclerosis.

[0020] Angelica sinensis is the dried root of the umbelliferae plant Angelica sinensis. It is sweet and spicy, warm in nature, and has the effects of nourishing blood, promoting blood circulation, regulating menstruation, etc. Modern pharmacology has found that Angelica sinensis has the effects of anti-thrombosis, improving coronary circulation, and lowering blood lipids. Current medicinal chemistry has found that Angelica sinensis is rich in ingredients such as ferulic acid, succinic acid, phthalic anhydride, anisic acid, ligustilide, and ligustilide. In the present invention, the organic acid components such as ferulic acid rich in Angelica sinensis are mainly used to improve mitochondrial respiratory function, oxidative phosphorylation efficiency, and promote mitochondrial ATP synthesis to repair mitochondrial function, enhance myocardial contractility, improve coronary circulation, and prevent and treat atherosclerosis.

[0021] Small yellow ginger is a variety of ginger, spicy, slightly warm, non-toxic, and has the effects of sweating, warming the middle and stopping vomiting, warming the lungs and stopping coughing. The small yellow ginger selected in the present invention is the fresh tuber of the perennial herbaceous plant of ginger family, small yellow ginger, rich in volatile oils such as gingerol, zingiberene, phellandrene, citral, linalool, and contains ingredients such as curcumin and gingerol. In the present invention, the significant antibacterial effect of volatile oil components such as gingerol, zingiberene, phellandrene, citral, linalool, etc. rich in small yellow ginger is mainly used to regulate intestinal flora disorders and inhibit the generation of intestinal toxic metabolites that damage mitochondrial function. At the same time, the curcumin and gingerol contained in small yellow ginger have significant hypolipidemic and antioxidant effects, inhibit the generation of reactive oxygen and lipid deposition, and eliminate the microenvironment of mitochondrial damage, high fat and high oxygen free radicals.

[0022] Single-headed garlic is the dried bulb of the Liliaceae plant, Allium sativum, which is warm in nature, spicy and sweet in taste, and has the effects of warming the stomach, promoting digestion and regulating qi. Single-headed garlic is rich in sulfides such as alliin, cycloalliin, allicin, alliin, and allicin. Among them, allicin and alliin are the main components of the volatile oil of single-headed garlic. Modern pharmacology has found that single-headed garlic not only has bactericidal and antibacterial effects, and is known as a "natural spectrum antibiotic", but also has the effects of lowering cholesterol in the body. In the present invention, on the one hand, the significant bactericidal and antibacterial effects of sulfides such as alliin, cycloalliin, allicin, alliin, and allicin in the unique garlic are used to regulate intestinal flora disorders and inhibit the generation of enterogenic toxic metabolites that damage mitochondrial function; on the other hand, these sulfides are used to improve mitochondrial respiratory function, oxidative phosphorylation efficiency, and promote mitochondrial ATP synthesis to repair mitochondrial function.

[0023] Antarctic krill oil is a mixed lipid extracted from Antarctic krill at low temperature. It is rich in phospholipids such as eicosapentaenoic acid, docosahexaenoic acid and other ω-3 polyunsaturated fatty acids, astaxanthin and other ingredients, and has significant effects such as anti-oxidation, anti-inflammatory and hypolipidemic. The present invention mainly utilizes the significant antioxidant and hypolipidemic effects of Antarctic krill oil to inhibit the generation of reactive oxygen species and lipid deposition, thereby eliminating the high-fat and high-oxygen free radical microenvironment of mitochondrial damage in the process of atherosclerosis.

[0024] Chitosan is a polyglucosamine (1-4)-2-amino-β-D glucose obtained by deacetylation of chitin, which is widely present in nature. Chitosan has a fibrous structure with many gaps inside and a large surface, so it has a very strong surface adsorption capacity due to the intermolecular attraction. At the same time, chitosan exhibits a significant antibacterial effect due to the presence of a large number of amino groups in its structure. In addition, the chitosan aqueous solution has a strong viscosity and exhibits a strong suspending effect. In the present invention, on the one hand, the antibacterial function of chitosan is used to regulate intestinal flora disorders and inhibit the generation of intestinal toxic metabolites that damage mitochondrial function; on the other hand, the adsorption function of chitosan is used to adsorb mitochondrial damage-inducing toxic metabolites produced by abnormal intestinal flora and promote the excretion of toxic metabolites. In addition, the suspension function of chitosan is also used to enhance the dispersion stability of the solid beverage in water.

[0025] Attapulgite, also known as palygorskite or palygorskite, is a hydrated magnesium-rich aluminum silicate clay mineral with a chain layered structure, and its structure belongs to a 2:1 type clay mineral. In each 2:1 unit structure layer, the corners of the tetrahedral wafers are reversed at a certain distance to form a layer chain. Channels parallel to the chain are formed between the tetrahedral strips, and the cross-section of the channel is about 3.7*6.3A°. The unique physical and chemical characteristics of attapulgite give it excellent adsorption, drug loading and dispersion properties. In the present invention, the adsorption, drug loading and dispersion effects of attapulgite are mainly utilized. On the one hand, chitosan is synergistically adsorbed and fixed to adsorb mitochondrial damage-inducing toxic metabolites produced by abnormal intestinal flora, and promote the excretion of toxic metabolites; on the other hand, its drug-adjuvant combination is used as an adsorbent to adsorb Antarctic krill oil, so that liquid components such as Antarctic krill oil can be solidified without adding an absorbent; at the same time, its dispersion function is used to enhance the stability of dispersion in water in the preparation of solid beverage products. In addition, it is also used to enhance the intestinal barrier function.

[0026] Stevia leaf is the leaf of Stevia rebaudiana, a plant of the Asteraceae family. The main component of Stevia leaf is steviol glycoside. Stevioside is not only highly sweet, 250 to 300 times sweeter than sucrose, but also is not absorbed by the human body and has low calories. Therefore, it is particularly suitable for people with high blood sugar and high blood fat. In the present invention, the high sweetness of steviol glycoside contained in Stevia leaf is mainly used to improve the taste of the product.

[0027] In the present invention, through the compatibility of various components, the effects of "regulating intestinal flora, reducing the generation of intestinal toxic metabolites that damage mitochondrial function; protecting intestinal barrier function, reducing intestinal absorption of intestinal mitochondrial toxic metabolites; improving mitochondrial respiratory function, increasing mitochondrial oxidative phosphorylation efficiency and promoting mitochondrial ATP synthesis; eliminating the high-fat and high-oxygen free radical microenvironment of mitochondrial damage" are synergistically exerted, thereby playing a significant role in repairing mitochondrial function in multiple ways and preventing and treating atherosclerosis. At the same time, through the compatibility of various components, not only the oily liquid component in the product is solidified, but also the product is given a higher dispersion stability in water, meeting the quality requirements of solid beverage formation and stability.

[0028] Beneficial effects: Compared with the prior art, the specific beneficial effects of the present invention are as follows: (1) In the present invention, Dendrobium officinale, Astragalus, Turmeric, Garlic and Chitosan are used to regulate intestinal flora disorder and inhibit the generation of enteric toxic metabolites that damage mitochondrial function. Dendrobium officinale, Astragalus and Attapulgite are used to synergistically enhance the intestinal barrier function and reduce the absorption of enteric toxic metabolites that damage mitochondrial function. Chitosan and Attapulgite are used to synergistically adsorb toxic metabolites that can induce mitochondrial damage produced by abnormal intestinal flora and promote the excretion of toxic metabolites. Astragalus, Angelica and Garlic are used to synergistically improve mitochondrial respiratory function, oxidative phosphorylation efficiency and promote mitochondrial ATP synthesis. Dendrobium officinale, Astragalus, Turmeric and Antarctic krill oil are used to synergistically inhibit the generation of reactive oxygen and lipid deposition and eliminate the high-fat and high-oxygen free radical microenvironment that damages mitochondria. Stevia is used to improve the taste of the product. The raw material formula formed by optimizing the combination can not only regulate the intestinal flora and reduce the production of enteric toxic metabolites that damage mitochondrial function, but also protect the intestinal barrier function, reduce the intestinal absorption of enteric mitochondrial damaging toxic metabolites, and eliminate the high-fat and high-oxygen free radical microenvironment of mitochondrial damage. At the same time, it can also improve mitochondrial respiratory function, increase mitochondrial oxidative phosphorylation efficiency and promote mitochondrial ATP synthesis, thereby achieving the purpose of preventing and treating atherosclerosis by repairing mitochondrial function in multiple ways.

[0029] (2) In the present invention, the volatile oil and other volatile active ingredients in garlic and turmeric are effectively retained in the product by physical homogenization and physical mixing at room temperature.

[0030] (3) In the present invention, while utilizing attapulgite to regulate the intestinal barrier function and absorb toxic metabolites produced by abnormal intestinal flora that can induce mitochondrial damage, it is combined with the drug excipient as an adsorbent to absorb Antarctic krill oil, thereby achieving the solidification of liquid components such as Antarctic krill oil without the addition of additional absorbents.

[0031] (4) In the present invention, the suspending or dispersing functions of Dendrobium officinale, chitosan and attapulgite are used to synergistically enhance the dispersion stability of the solid beverage in water, thereby meeting the requirements of solid beverage molding and stability. DETAILED DESCRIPTION

[0032] The present invention is further described in detail below in conjunction with the examples, but the embodiments of the present invention are not limited thereto. The parts described in the examples are all parts by mass.

[0033] The effective component content of the solid beverage for preventing and treating atherosclerosis is: total saponin ≥100 μg / g, ferulic acid ≥20 μg / g, total flavonoids ≥30 mg / g, total polysaccharides ≥250 mg / g, and volatile oil ≥10 mg / g. After being dispersed in water, the sedimentation ratio is ≥0.92 within 3 hours.

[0034] Example 1 (1) Raw material collection and pretreatment Take the commercially available dried roots of Mongolian Astragalus and grind them into powder until they can pass through a 60-mesh sieve.

[0035] Take the dried roots of Angelica sinensis available on the market and grind them into powder until they can pass through a 60-mesh sieve.

[0036] Take commercially available fresh turmeric, wash and drain, peel and cut into 1 cm dices.

[0037] Take the single-headed garlic from the market, peel it and remove impurities, then wash and drain.

[0038] Take three-year-old medicinal-grade Dendrobium officinale leaves from the market and crush them until they pass through an 80-mesh sieve.

[0039] Take commercially available stevia and grind it to pass through an 80-mesh sieve.

[0040] Take commercially available chitosan with a molecular weight of 50-90 kDa and grind it to pass through an 80-mesh sieve.

[0041] Take commercially available pharmaceutical adsorption grade attapulgite and grind it to pass through an 80-mesh sieve.

[0042] (2) Preparation of mixed extract of Astragalus and Angelica The pre-treated astragalus and angelica raw materials were weighed according to the mass parts of 30 parts of astragalus and 6 parts of angelica, fully mixed and placed in an extraction device, deionized water was added in a ratio of 1:10 of powder to deionized water, soaked for 30 minutes, refluxed at normal pressure for 90 minutes, and filtered. The filter residue was decocted once again under the same conditions and filtered. After combining the two filtrates, they were concentrated under reduced pressure to a relative density of 0.4 under the conditions of a vacuum degree of 0.090 pa and a temperature of 60 degrees Celsius to obtain a mixed extract of astragalus and angelica.

[0043] (3) Preparation of a mixed homogenate of turmeric and garlic with a mixed extract of astragalus and angelica Weigh the pretreated small yellow turmeric and single garlic pretreated raw materials, according to the mass parts of 10 parts of small yellow turmeric and 10 parts of single garlic, weigh the small yellow turmeric and single garlic raw materials, put them in a homogenizer, add the prepared astragalus and angelica mixed extract, and fully homogenize with the homogenizer until the slurry passes through an 80-mesh sieve to obtain a mixed homogenate of small yellow turmeric and single garlic and the astragalus and angelica mixed extract.

[0044] (4) Preparation of a mixture of Antarctic krill oil and attapulgite Weigh 5 parts of pretreated attapulgite and 10 parts of Antarctic krill oil, spray the Antarctic krill oil evenly on the attapulgite powder, mix thoroughly, and place in a sealed container for 6 hours to obtain a mixture of Antarctic krill oil and attapulgite.

[0045] (5) Preparation of a mixture of chitosan, turmeric, garlic, astragalus, angelica, Antarctic krill oil, and attapulgite Weigh 3 parts of pretreated chitosan, add it to the mixed homogenate of turmeric and garlic, astragalus and angelica extract, stir and suspend it thoroughly, then evenly add it to the mixture of Antarctic krill oil and attapulgite by passing it through an 80-mesh sieve, stir and mix it thoroughly to obtain a mixture of chitosan, turmeric, garlic, astragalus, angelica, Antarctic krill oil and attapulgite.

[0046] (6) Preparation of mixed powder of Dendrobium officinale and Stevia rebaudiana The pretreated raw materials of Dendrobium officinale and Stevia rebaudiana were weighed according to 40 parts of Dendrobium officinale and 3 parts of Stevia rebaudiana by mass, and the two were mixed evenly by equal increasing method to obtain a mixed powder of Dendrobium officinale and Stevia rebaudiana.

[0047] (7) Formation of solid beverage products for preventing and treating atherosclerosis The prepared chitosan, turmeric, garlic, astragalus, angelica, Antarctic krill oil and attapulgite mixture is added to the mixed powder of Dendrobium officinale and Stevia in equal amounts, and after being fully mixed, passed through an 80-mesh sieve to obtain a solid beverage product for preventing and treating atherosclerosis.

[0048] (8) Product quality evaluation The solid beverage for preventing and treating atherosclerosis is a yellow-brown solid powder, and no oil infiltration is observed by oil absorbing paper. The content of the effective ingredient of the solid beverage for preventing and treating atherosclerosis was determined, and the results showed that the total saponin of the product obtained in this embodiment was 155.45 ± 9.19 μg / g, the ferulic acid was 37.99 ± 1.92 μg / g, the total flavonoids was 41.45 ± 3.74 mg / g, the total polysaccharide was 305.45 ± 17.24 mg / g, and the volatile oil was 14.15 ± 1.32 mg / g. After dispersion in water, no free oil was observed, and the sedimentation ratio within 3 hours was 0.954 ± 0.013.

[0049] Example 2 (1) Raw material collection and pretreatment Take the commercially available dried root of Astragalus membranaceus and grind it into powder until it passes through a 60-mesh sieve.

[0050] Take the dried roots of Angelica sinensis available on the market and grind them into powder until they can pass through a 60-mesh sieve.

[0051] Take commercially available fresh turmeric, wash and drain, peel and cut into 1 cm dices.

[0052] Take the single-headed garlic from the market, peel it and remove impurities, then wash and drain.

[0053] Take three-year-old medicinal-grade Dendrobium officinale leaves from the market and grind them to pass through a 120-mesh sieve.

[0054] Take commercially available stevia and grind it to pass through a 120-mesh sieve.

[0055] Take commercially available chitosan with a molecular weight of 50-90 kDa and grind it to pass through a 120-mesh sieve.

[0056] Take commercially available pharmaceutical adsorption grade attapulgite and grind it to pass through a 120-mesh sieve.

[0057] (2) Preparation of mixed extract of Astragalus and Angelica The pre-treated astragalus and angelica raw materials were weighed according to the mass parts of 50 parts of astragalus and 10 parts of angelica, fully mixed and placed in an extraction device, deionized water was added in a ratio of 1:10 of powder to deionized water, soaked for 30 minutes, refluxed at normal pressure for 90 minutes, and filtered. The filter residue was decocted once again under the same conditions and filtered. After combining the two filtrates, they were concentrated under reduced pressure to a relative density of 0.8 under the conditions of a vacuum degree of 0.098 pa and a temperature of 80 degrees Celsius to obtain a mixed extract of astragalus and angelica.

[0058] (3) Preparation of a mixed homogenate of turmeric and garlic with a mixed extract of astragalus and angelica Weigh the pretreated small yellow turmeric and single garlic pretreated raw materials, according to the mass parts of 15 parts of small yellow turmeric and 15 parts of single garlic, weigh the small yellow turmeric and single garlic raw materials, put them in a homogenizer, add the prepared astragalus and angelica mixed extract, and fully homogenize with the homogenizer until the slurry passes through a 120-mesh sieve to obtain a mixed homogenate of small yellow turmeric and single garlic and the astragalus and angelica mixed extract.

[0059] (4) Preparation of a mixture of Antarctic krill oil and attapulgite Weigh 10 parts of pretreated attapulgite and 20 parts of Antarctic krill oil, spray the Antarctic krill oil evenly on the attapulgite powder, mix thoroughly, and place in a sealed container for 9 hours to obtain a mixture of Antarctic krill oil and attapulgite.

[0060] (5) Preparation of a mixture of chitosan, turmeric, garlic, astragalus, angelica, Antarctic krill oil, and attapulgite Weigh 6 portions of pretreated chitosan, add it to the homogenate of the mixed extract of turmeric and garlic, astragalus and angelica, stir and suspend it thoroughly, then evenly add it to the mixture of Antarctic krill oil and attapulgite by passing it through a 120-mesh sieve, stir and mix it thoroughly to obtain a mixture of chitosan, turmeric, garlic, astragalus, angelica, Antarctic krill oil and attapulgite.

[0061] (6) Preparation of mixed powder of Dendrobium officinale and Stevia rebaudiana The pretreated raw materials of Dendrobium officinale and Stevia rebaudiana were weighed according to 60 parts of Dendrobium officinale and 6 parts of Stevia rebaudiana by mass, and the two were mixed evenly by equal increasing method to obtain a mixed powder of Dendrobium officinale and Stevia rebaudiana.

[0062] (7) Formation of solid beverage products for preventing and treating atherosclerosis The prepared chitosan, turmeric, garlic, astragalus, angelica, Antarctic krill oil and attapulgite mixture is added to the mixed powder of Dendrobium officinale and stevia in equal and increasing amounts, and after being fully mixed, the mixture is passed through a 120-mesh sieve to obtain a solid beverage product for preventing and treating atherosclerosis.

[0063] (8) Product quality evaluation The solid beverage for preventing and treating atherosclerosis is a yellow-brown solid powder, and no oil infiltration is observed by oil absorbing paper. The content of the effective ingredient of the solid beverage for preventing and treating atherosclerosis was determined, and the results showed that the total saponin of the product obtained in this embodiment was 201.31 ± 8.43 μg / g, the ferulic acid was 58.11 ± 2.45 μg / g, the total flavonoids was 67.69 ± 4.12 mg / g, the total polysaccharide was 435.64 ± 21.34 mg / g, and the volatile oil was 20.57 ± 1.92 mg / g. After dispersion in water, no free oil was observed, and the sedimentation ratio was 0.982 ± 0.011 within 3 hours.

[0064] Example 3 (1) Raw material collection and pretreatment Take the commercially available dried roots of Mongolian Astragalus and grind them into powder until they can pass through a 60-mesh sieve.

[0065] Take the dried roots of Angelica sinensis available on the market and grind them into powder until they can pass through a 60-mesh sieve.

[0066] Take commercially available fresh turmeric, wash and drain, peel and cut into 1 cm dices.

[0067] Take the single-headed garlic from the market, peel it and remove impurities, then wash and drain.

[0068] Take three-year-old medicinal-grade Dendrobium officinale leaves from the market and grind them to pass through a 100-mesh sieve.

[0069] Take commercially available stevia and grind it to pass through a 100-mesh sieve.

[0070] Take commercially available chitosan with a molecular weight of 50-90 kDa and grind it to pass through a 100-mesh sieve.

[0071] Take commercially available pharmaceutical adsorption grade attapulgite and grind it to pass through a 100-mesh sieve.

[0072] (2) Preparation of mixed extract of Astragalus and Angelica The pre-treated astragalus and angelica raw materials were weighed according to the mass parts of 40 parts of astragalus and 8 parts of angelica, fully mixed and placed in an extraction device, deionized water was added in a ratio of 1:10 of powder to deionized water, soaked for 30 minutes, refluxed at normal pressure for 90 minutes, and filtered. The filter residue was decocted once again under the same conditions and filtered. After combining the two filtrates, they were concentrated under reduced pressure to a relative density of 0.6 under the conditions of a vacuum degree of 0.095 pa and a temperature of 70 degrees Celsius to obtain a mixed extract of astragalus and angelica.

[0073] (3) Preparation of a mixed homogenate of turmeric and garlic with a mixed extract of astragalus and angelica Weigh the pretreated small yellow turmeric and single garlic pretreated raw materials, according to the mass parts of 13 parts of small yellow turmeric and 13 parts of single garlic, weigh the small yellow turmeric and single garlic raw materials, put them in a homogenizer, add the prepared astragalus and angelica mixed extract, and fully homogenize with the homogenizer until the slurry passes through a 100-mesh sieve to obtain a mixed homogenate of small yellow turmeric and single garlic and the astragalus and angelica mixed extract.

[0074] (4) Preparation of a mixture of Antarctic krill oil and attapulgite Weigh 8 parts of pre-treated attapulgite and 15 parts of Antarctic krill oil, spray the Antarctic krill oil evenly on the attapulgite powder, mix thoroughly, and place in a sealed container for 8 hours to obtain a mixture of Antarctic krill oil and attapulgite.

[0075] (5) Preparation of a mixture of chitosan, turmeric, garlic, astragalus, angelica, Antarctic krill oil, and attapulgite Weigh 4.5 parts of pretreated chitosan, add it to the mixed homogenate of turmeric and garlic, astragalus and angelica extract, stir and suspend it thoroughly, then evenly add it to the mixture of Antarctic krill oil and attapulgite by passing it through a 100-mesh sieve, stir and mix thoroughly to obtain a mixture of chitosan, turmeric, garlic, astragalus, angelica, Antarctic krill oil and attapulgite.

[0076] (6) Preparation of mixed powder of Dendrobium officinale and Stevia rebaudiana The pretreated raw materials of Dendrobium officinale and Stevia rebaudiana were weighed according to the mass proportion of 50 parts of Dendrobium officinale and 4.5 parts of Stevia rebaudiana, and the two materials were mixed evenly by equal increasing method to obtain a mixed powder of Dendrobium officinale and Stevia rebaudiana.

[0077] (7) Formation of solid beverage products for preventing and treating atherosclerosis The prepared chitosan, turmeric, garlic, astragalus, angelica, Antarctic krill oil and attapulgite mixture is added to the mixed powder of Dendrobium officinale and stevia in equal and increasing amounts, and after being fully mixed, passed through a 100-mesh sieve to obtain a solid beverage product for preventing and treating atherosclerosis.

[0078] (8) Product quality evaluation The solid beverage for preventing and treating atherosclerosis is a yellow-brown solid powder, and no oil infiltration is observed by oil absorbing paper. The content of the effective ingredient of the solid beverage for preventing and treating atherosclerosis was determined, and the results showed that the total saponin of the product obtained in this embodiment was 183.12 ± 5.77 μg / g, the ferulic acid was 46.56 ± 0.95 μg / g, the total flavonoids was 57.18 ± 5.11 mg / g, the total polysaccharide was 361.92 ± 12.96 mg / g, and the volatile oil was 16.74 ± 0.75 mg / g. After dispersion in water, no free oil was observed, and the sedimentation ratio was 0.969 ± 0.012 within 3 hours.

[0079] Effect verification example (1) Verification of the effect and mechanism of solid beverage products for the prevention and treatment of atherosclerosis on improving mitochondrial dysfunction and atherosclerotic lesions in atherosclerosis model mice: 50 SPF-grade ApoE - / - The mice were fed with normal feed in the animal quarantine room for 1 week. If there were no abnormalities after 1 week, they were transferred to the feeding room for group intervention. - / - The mice were randomly divided into 5 groups, namely, blank control group, model group, Example 1 group, Example 2 group, and Example 3 group, with 10 mice in each group, half male and half female. The blank control group was fed with ordinary feed (basic feed), and the other groups were fed with high-fat feed (21% fat, 0.25% cholesterol, 1% choline, 77.75% basic feed), and they were free to eat and drink water. At the same time, Example 1 group, Example 2 group, and Example 3 group were intragastrically administered with 0.1 mL / 10 g of physiological saline solution of different embodiment products (according to the set dosage for administration, each embodiment product was dissolved in physiological saline). The blank control group and the model group were given the same volume of physiological saline. The intervention lasted for 12 weeks. 24 hours after the last administration, the eyeballs were removed after anesthesia to obtain blood, and then the mice were killed by dislocation of the cervical vertebra. The colon, intestinal contents, and thoracic aorta were quickly dissected.

[0080] The composition of intestinal flora was analyzed by 16S rDNA sequencing technology, and the content of indole and trimethylamine in intestinal contents was determined by ultra-high performance liquid chromatography to evaluate the effect of solid beverage products for preventing and treating atherosclerosis on regulating intestinal flora disorder and inhibiting the production of enteric toxic metabolites that damage mitochondrial function. The expression of intestinal junction protein ZO-1 was determined by immunohistochemical staining, and the content of indoxyl sulfate and trimethylamine oxide in serum was determined by ultra-high performance liquid chromatography to evaluate the effect of solid beverage products for preventing and treating atherosclerosis on enhancing intestinal barrier function and reducing the absorption of enteric toxic metabolites that damage mitochondrial function. The total cholesterol, high-density lipoprotein and low-density lipoprotein levels in serum were analyzed by kits, and on this basis, the atherosclerosis index (AI) was calculated; the plaque size of thoracic aorta sections was determined and evaluated by Oil Red O staining, and the ratio of plaque to lumen (P / L%) was calculated to evaluate the effect of solid beverage products for preventing and treating atherosclerosis on improving atherosclerotic lesions. The Clark method was used to isolate the mitochondria of the thoracic aorta, and the respiratory control ratio (RCR), oxidative phosphorylation efficiency (OPR) and ATP content of the mitochondria of the thoracic aorta of each group were measured and evaluated to evaluate the effect of solid beverage products for the prevention and treatment of atherosclerosis on mitochondrial function. The triglyceride (TC) content in serum was analyzed using a kit, and the reactive oxygen species (ROS) level of thoracic aortic mitochondria was determined by MitoSOXTMRed probe fluorescence staining to evaluate the role of solid beverage products for the prevention and treatment of atherosclerosis in eliminating the high-fat and high-oxygen free radical microenvironment of mitochondrial damage. The results are shown in Table 1.

[0081]

[0082] Note: 1. a means relative to the model group. P＜0.05 : b means relative to the model group, P＜0.01 .

[0083] 2. The dosage of each example is calculated based on the equivalent dosage ratio of 0.0026 between humans and mice based on body surface area. The dosage of humans is 5 g / d, and the dosage of mice after conversion is 650 mg / kg / d.

[0084] As shown in the experimental results of Table 1, relative to the blank control group, the relative abundance of harmful bacteria of Firmicutes in the model group mice significantly increased, while the relative abundance of beneficial bacteria of Bacteroidetes significantly decreased, and the content of indole and trimethylamine in the intestinal contents also significantly increased. It shows that the intestinal flora of the model group mice was disordered, which promoted the generation of indole and trimethylamine, the intestinal toxic metabolites that damaged mitochondrial function. However, relative to the model group, in each embodiment group, after simultaneously giving the solid beverage product for the prevention and treatment of atherosclerosis, the relative abundance of harmful bacteria of Firmicutes was significantly reduced, while the relative abundance of beneficial bacteria of Bacteroidetes was significantly increased, and the content of indole and trimethylamine in the intestinal contents also significantly decreased. These results show that the solid beverage product for the prevention and treatment of atherosclerosis obtained in each embodiment has the effect of regulating intestinal flora disorder and inhibiting the generation of intestinal toxic metabolites that damage mitochondrial function.

[0085] The experimental results in Table 1 show that, compared with the blank control group, the expression rate of intestinal junction protein ZO-1 in the model group mice was significantly decreased, and the contents of indoxyl sulfate and trimethylamine oxide in the serum were also significantly increased. This indicates that the damage to the intestinal barrier function of the model group mice promoted the absorption of indole and trimethylamine, the intestinal toxic metabolites that damage mitochondrial function (indoxyl sulfate and trimethylamine oxide are the conversion products after indole and trimethylamine are absorbed). However, compared with the model group, the expression rate of ZO-1 in each embodiment group was significantly increased after the solid beverage product for preventing and treating atherosclerosis was given at the same time, and the contents of indoxyl sulfate and trimethylamine oxide in the serum were also significantly decreased. These results show that the solid beverage product for preventing and treating atherosclerosis obtained in each embodiment has the effect of enhancing the intestinal barrier function and reducing the absorption of intestinal toxic metabolites that damage mitochondrial function.

[0086] The experimental results in Table 1 show that, compared with the blank control group, the AI ​​value and P / L% of the mice in the model group were significantly increased, and the AI ​​value of the model group was much greater than the diagnostic value of 4.0 for atherosclerosis, indicating that the mice in the model group were in a state of severe atherosclerotic lesions. However, compared with the model group, the AI ​​value and P / L% of each embodiment group significantly decreased after the solid beverage product for preventing and treating atherosclerosis was given at the same time, and the AI ​​value of each embodiment group was significantly lower than the diagnostic value of 4.0 for atherosclerosis. These results show that the solid beverage product for preventing and treating atherosclerosis obtained in each embodiment has a significant effect of preventing and treating atherosclerosis.

[0087] It can be seen from the experimental results in Table 1 that, compared with the blank control group, the RCR, OPR and ATP content of the mice in the model group were significantly decreased. It shows that the mitochondrial function of the thoracic aorta of the mice in the model group has been significantly reduced. However, compared with the model group, after the solid beverage product for preventing and treating atherosclerosis was given at the same time, the RCR, OPR and ATP content of each embodiment group were significantly increased. These results show that the solid beverage product for preventing and treating atherosclerosis obtained in each embodiment can significantly improve the respiratory function of the thoracic aorta mitochondria, improve the efficiency of oxidative phosphorylation and promote ATP synthesis, thereby showing a significant repair effect on mitochondrial dysfunction.

[0088] The experimental results in Table 1 confirmed that the TC and mitochondrial ROS levels of the model group mice were significantly increased compared with the blank control group. This indicates that the model group mice were in a state of high fat and high oxidative stress damage to mitochondria. However, compared with the model group, the serum TC and mitochondrial ROS levels of each embodiment group were significantly decreased after the solid beverage product for preventing and treating atherosclerosis was given at the same time. These results show that the solid beverage product for preventing and treating atherosclerosis obtained in each embodiment has the effect of eliminating the high fat and high oxygen free radical microenvironment of mitochondrial damage.

[0089] In summary, the results in Table 1 confirm that the solid beverage product for preventing and treating atherosclerosis obtained by the present invention can repair mitochondrial function through multiple pathways such as "regulating intestinal flora, inhibiting the production of enteric toxic metabolites that damage mitochondrial function; enhancing intestinal barrier function, reducing intestinal absorption of enteric toxic metabolites that damage mitochondria; improving mitochondrial respiratory function, increasing mitochondrial oxidative phosphorylation efficiency and promoting mitochondrial ATP synthesis; eliminating the high-fat and high-oxygen free radical microenvironment that damages mitochondria", thereby showing the effect of preventing and treating atherosclerosis.

[0090] (2) Verification of the preparation method for the retention of volatile oils in garlic and turmeric The product yield of the solid beverage for preventing and treating atherosclerosis in each embodiment was calculated. 50 g of each embodiment product was weighed and placed in different volatile oil extractors to extract volatile oil. At the same time, according to the product yield of each embodiment and the ratio of small yellow turmeric and single-headed garlic raw materials in the prescription, the amount of small yellow turmeric and single-headed garlic raw materials corresponding to each embodiment product was calculated. On this basis, small yellow turmeric and single-headed garlic raw materials in the same proportion and amount as the products of each embodiment were weighed, mixed, and then placed in different volatile oil extractors with the corresponding embodiment samples to extract volatile oil. The difference in volatile oil between the products of each embodiment and the corresponding proportion and amount of small yellow turmeric and single-headed garlic mixed raw materials was compared to verify the retention of volatile oil of single-headed garlic and small yellow turmeric by the preparation method. The results are shown in Table 2.

[0091]

[0092] Note: ns means that the difference is not significant compared with the mixture of turmeric and garlic in the same proportion and amount.

[0093] From the results in Table 2, it can be seen that compared with the raw material mixture of small yellow turmeric and single-headed garlic in the same proportion and amount, the volatile oil content of the product obtained by the preparation method of the present invention is slightly lower, but the difference does not reach a significant level. It shows that the product can effectively retain the volatile oil of single-headed garlic and small yellow turmeric.

[0094] (3) Verification of the beneficial effects of each component in the formula of the present invention Take Example 1 as a control. According to the same formula ratio as Example 1, each component raw material in the formula is knocked out (evaluation component removed) and knocked in (increasing the proportion of the evaluation component within the rated mass ratio of each component in this formula) to form the following component knock-out and knock-in products: Component knock-out and knock-in formula 1 Product formula: by mass, 30 parts of astragalus, 6 parts of angelica, 10 parts of small yellow turmeric, 10 parts of single-headed garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out and knock-in formula 2 product formula: by mass, 55 parts of Dendrobium candidum, 30 parts of Astragalus, 6 parts of Angelica sinensis, 10 parts of small yellow turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out and knock-in formula 3 product formula: by mass, 40 parts of Dendrobium officinale, 6 parts of Angelica sinensis, 10 parts of small yellow turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out and knock-in formula 4 product formula: by mass, 40 parts of Dendrobium officinale, 45 parts of Astragalus, 6 parts of Angelica sinensis, 10 parts of small yellow turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out and knock-in formula 5 product formula: by mass, 40 parts of Dendrobium candidum, 30 parts of Astragalus, 10 parts of small yellow turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out and knock-in formula 6 product formula: by mass, 40 parts of Dendrobium candidum, 30 parts of Astragalus, 9 parts of Angelica sinensis, 10 parts of small yellow turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out and knock-in formula 7 product formula: by mass, 40 parts of Dendrobium officinale, 30 parts of Astragalus, 6 parts of Angelica sinensis, 10 parts of Garlic lily, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of Stevia; Component knock-out and knock-in formula 8 product formula: by mass, 40 parts of Dendrobium officinale, 30 parts of Astragalus, 6 parts of Angelica sinensis, 14 parts of small yellow turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out and knock-in formula 9 product formula: by mass, 40 parts of Dendrobium officinale, 30 parts of Astragalus, 6 parts of Angelica sinensis, 10 parts of Rhizoma Zingiberis, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of Stevia; The formula of the knock-out and knock-in formula 10 is as follows: by mass: 40 parts of Dendrobium candidum, 30 parts of Astragalus, 6 parts of Angelica sinensis, 10 parts of Gingerbread, 14 parts of Garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of Attapulgite, and 3 parts of Stevia; Component knock-out and knock-in formula 11 product formula: by mass, 40 parts of Dendrobium officinale, 30 parts of Astragalus, 6 parts of Angelica sinensis, 10 parts of small yellow turmeric, 10 parts of garlic, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out and knock-in formula 12 product formula: by mass, 40 parts of Dendrobium officinale, 30 parts of Astragalus, 6 parts of Angelica sinensis, 10 parts of small yellow turmeric, 10 parts of garlic, 18 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out and knock-in formula 13 product formula: by mass, 40 parts of Dendrobium officinale, 30 parts of Astragalus, 6 parts of Angelica sinensis, 10 parts of small yellow turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out and knock-in formula 14 product formula: by mass, 40 parts of Dendrobium officinale, 30 parts of Astragalus, 6 parts of Angelica sinensis, 10 parts of small yellow turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 5 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out and knock-in formula 15 product formula: by mass, 40 parts of Dendrobium officinale, 30 parts of Astragalus, 6 parts of Angelica sinensis, 10 parts of small yellow turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, and 3 parts of stevia; Component knock-out and knock-in formula 16 product formula: by mass, 40 parts of Dendrobium officinale, 30 parts of Astragalus, 6 parts of Angelica sinensis, 10 parts of small yellow turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 9 parts of attapulgite, and 3 parts of stevia; Component knock-out and knock-in formula 17 product formula: by mass, 40 parts of Dendrobium officinale, 30 parts of Astragalus, 6 parts of Angelica sinensis, 10 parts of small yellow turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, and 5 parts of attapulgite; Component knock-out and knock-in formula 18 product formula: by mass, 40 parts of Dendrobium officinale, 30 parts of Astragalus, 6 parts of Angelica sinensis, 10 parts of small yellow turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 5 parts of stevia.

[0095] According to the same preparation process as Example 1, the knock-out and knock-in products of each component were prepared, and a total of 18 different knock-out and knock-in products of each component were obtained. Using the random double-blind principle, 20 healthy volunteers were selected to observe the morphology of each product, and the oil-absorbing paper infiltration after adsorbing each component knock-out and knock-in product and the product of Example 1 with oil-absorbing paper was observed respectively. At the same time, each product was dispersed in water to observe the free oil situation, and the liquid component solidification and adsorption fixation effect of Antarctic krill oil were evaluated. The knock-out and knock-in products of each component and the product of Example 1 were tasted by healthy volunteers for taste scoring. The scoring standard is: 4 points for sweet, 3 points for slightly sweet, 2 points for not sweet and no other bad taste, 1 point for not sweet and slightly other bad taste, and 0 points for not sweet and obvious other bad taste. The taste of the product is evaluated according to the average value of the taste test scores of all volunteers. The higher the score, the more palatable it is.

[0096] 190 SPF-grade ApoE - / - The mice were fed with normal feed in the animal quarantine room for 1 week. If there were no abnormalities after 1 week, they were transferred to the feeding room for group intervention. - / - The mice were randomly divided into 19 groups, namely, Example 1 group, component knock-out and knock-in group 1 to component knock-out and knock-in group 18, with 10 mice in each group, half male and half female. Each group was fed with a high-fat diet (21% fat, 0.25% cholesterol, 1% choline, 77.75% basal feed) and had free access to food and water. At the same time, each group was intragastrically administered with 0.1 mL / 10 g of a physiological saline solution of the product of Example 1 and the products of each component knock-out and knock-in (the product of Example 1 and the products of each component knock-out and knock-in were dissolved in physiological saline according to the set dosage for administration). The intervention lasted for 12 weeks. 24 hours after the last administration, the eyeballs were removed after anesthesia to obtain blood, and then the mice were killed by dislocating the cervical vertebra. The colon, intestinal contents and thoracic aorta were quickly dissected.

[0097]

[0098]

[0099]

[0100] The composition of intestinal flora in the intestinal contents was analyzed by 16S rDNA sequencing technology, and the contents of indole and trimethylamine in the intestinal contents were determined by ultra-high performance liquid chromatography to evaluate the effect of solid beverage products for preventing and treating atherosclerosis on regulating intestinal flora disorder and inhibiting the production of enteric toxic metabolites that damage mitochondrial function. The expression of intestinal junction protein ZO-1 was determined by immunohistochemical staining, and the contents of indoxyl sulfate and trimethylamine oxide in serum were determined by ultra-high performance liquid chromatography to evaluate the effect of solid beverage products for preventing and treating atherosclerosis on enhancing intestinal barrier function and reducing the absorption of enteric toxic metabolites that damage mitochondrial function. The total cholesterol, high-density lipoprotein and low-density lipoprotein contents in serum were analyzed by kits, and the AI ​​value was calculated on this basis; the plaque size of thoracic aorta sections was determined and evaluated by Oil Red O staining, and the P / L% value was calculated to evaluate the effect of solid beverage products for preventing and treating atherosclerosis on improving atherosclerosis. The mitochondria of the thoracic aorta were separated by Clark's method, and the RCR, OPR and ATP contents of the mitochondria of the thoracic aorta in each group were determined and evaluated to evaluate the effect of solid beverage products for preventing and treating atherosclerosis on mitochondrial function. The TC content in serum was analyzed by a kit, and the ROS level in the mitochondria of the thoracic aorta was determined by MitoSOXTM Red probe fluorescence staining method to evaluate the effect of solid beverage products for preventing and treating atherosclerosis in eliminating the high-fat and high-oxygen free radical microenvironment of mitochondrial damage. The results are shown in Table 3.

[0101] The experimental results in Table 3 reveal that, compared with the product in Example 1, the relative abundance of harmful bacteria of Firmicutes in the component knock-out and knock-in products formed after knocking out Dendrobium officinale in the formula was significantly increased, and the relative abundance of beneficial bacteria of Bacteroidetes was significantly reduced; the content of indole and trimethylamine in the intestinal contents was also significantly increased; the expression rate of intestinal junction protein ZO-1 was significantly decreased, and the content of indoxyl sulfate and trimethylamine oxide in serum was also significantly increased; the AI ​​value and P / L% were also significantly increased. At the same time, TC and ROS levels were also significantly increased. In addition, the sedimentation ratio of the product after dispersion in water was also significantly reduced. However, the abnormal parameters of the component knock-out and knock-in products 1, which were significantly reduced or increased relative to the product in Example 1, reflecting the weakening of the product's efficacy and performance, were significantly better than those of the product in Example 1 when Dendrobium officinale was knocked into the formula to increase its proportion. These results suggest that compared with the product of Example 1, the knock-out and knock-in products of Formula 1, in which the components formed by Dendrobium officinale are removed from the formula, have significantly weakened effects on regulating the disorder of intestinal flora of mice induced by high fat, inhibiting the production of intestinal toxic metabolites indole and trimethylamine that damage mitochondrial function, enhancing intestinal barrier function, reducing the absorption of intestinal toxic metabolites that damage mitochondrial function, preventing and treating atherosclerosis, eliminating the high-fat and high-oxygen free radical microenvironment that damages mitochondria, and increasing the water-dispersible suspension stability of the product. However, the knock-out and knock-in products of Formula 2, in which the components formed by Dendrobium officinale are added to the formula, have significantly enhanced effects on regulating the disorder of intestinal flora of mice induced by high fat, inhibiting the production of intestinal toxic metabolites indole and trimethylamine that damage mitochondrial function, enhancing intestinal barrier function, reducing the absorption of intestinal toxic metabolites that damage mitochondrial function, preventing and treating atherosclerosis, eliminating the high-fat and high-oxygen free radical microenvironment that damages mitochondrial function, and increasing the water-dispersible suspension stability of the product. These results indicate that Dendrobium officinale in the present invention has the effects of "regulating the disorder of intestinal flora of mice induced by high fat, inhibiting the production of intestinal toxic metabolites indole and trimethylamine that damage mitochondrial function, enhancing intestinal barrier function, reducing the absorption of intestinal toxic metabolites that damage mitochondrial function, preventing and treating atherosclerosis, eliminating the high-fat and high-oxygen free radical microenvironment that damages mitochondria, and increasing the stability of the product's water-dispersible suspension."

[0102] The experimental results of Table 3 show that, compared with the product of Example 1, the relative abundance of harmful bacteria of Firmicutes in the component knock-out and knock-in party 3 product formed after knocking out Astragalus in the prescription was significantly increased, and the relative abundance of beneficial bacteria of Bacteroidetes was significantly reduced; the content of indole and trimethylamine in the intestinal contents was also significantly increased; the expression rate of intestinal junction protein ZO-1 was significantly decreased, and the content of indoxyl sulfate and trimethylamine oxide in serum was also significantly increased; AI value and P / L% were also significantly increased. At the same time, the mitochondrial RCR, OPR and ATP content decreased significantly. TC and ROS levels were also significantly increased. However, the abnormal parameters of the component knock-out and knock-in party 3 product, which were significantly reduced or increased relative to the product of Example 1, reflecting the weakening of the product's efficacy and performance, were obtained when Astragalus was knocked into the prescription to increase its proportion. Compared with the product of Example 1, these parameters were significantly better than those of Example 1. These results suggest that compared with the product in Example 1, the knock-out and knock-in products of the formula 3, which remove the component formed by Astragalus in the formula, have significantly weakened the effects of regulating the disorder of intestinal flora of mice induced by high fat, inhibiting the production of intestinal toxic metabolites indole and trimethylamine that damage mitochondrial function, enhancing intestinal barrier function, reducing the absorption of intestinal toxic metabolites that damage mitochondrial function, preventing and treating atherosclerosis, improving mitochondrial respiratory function and oxidative phosphorylation efficiency, and promoting mitochondrial ATP synthesis to repair mitochondrial function, and eliminating the high-fat and high-oxygen free radical microenvironment of mitochondrial damage. However, the knock-out and knock-in products of the formula 4, which add the component formed by Astragalus in the formula, have significantly enhanced the effects of regulating the disorder of intestinal flora of mice induced by high fat, inhibiting the production of intestinal toxic metabolites indole and trimethylamine that damage mitochondrial function, enhancing intestinal barrier function, reducing the absorption of intestinal toxic metabolites that damage mitochondrial function, preventing and treating atherosclerosis, improving mitochondrial respiratory function and oxidative phosphorylation efficiency, and promoting mitochondrial ATP synthesis to repair mitochondrial function, and eliminating the high-fat and high-oxygen free radical microenvironment of mitochondrial damage. These results indicate that Astragalus in the present invention has the effects of "regulating the disorder of intestinal flora in mice induced by high fat, inhibiting the production of intestinal toxic metabolites indole and trimethylamine that damage mitochondrial function, enhancing intestinal barrier function, reducing the absorption of intestinal toxic metabolites that damage mitochondrial function, preventing and treating atherosclerosis, improving mitochondrial respiratory function, oxidative phosphorylation efficiency and promoting mitochondrial ATP synthesis to repair mitochondrial function and eliminate the high-fat and high-oxygen free radical microenvironment that damages mitochondria".

[0103] The experimental results of Table 3 show that, compared with the product of Example 1, when the component knocked out and knocked into the square 5 product formed after knocking out the angelica in the prescription, its mitochondrial RCR, OPR and ATP content are significantly reduced, but the AI ​​value and P / L% are significantly increased. However, the abnormal parameters that reflect the weakening of the product efficacy, which are significantly reduced or increased by the component knocking out and knocking into the square 5 product relative to the product of Example 1, are obtained after the component knocking out and knocking into the square 6 product to increase its ratio in the prescription, and these parameters are significantly better than the product of Example 1. These results suggest that, compared with the product of Example 1, the component knocking out and knocking into the square 5 product formed by removing the angelica in the prescription, which improves mitochondrial respiratory function, oxidative phosphorylation efficiency and promotes mitochondrial ATP synthesis to repair mitochondrial function, is significantly weakened. However, the component knocking out and knocking into the square 6 product formed by increasing the angelica in the prescription, improves mitochondrial respiratory function, oxidative phosphorylation efficiency and promotes mitochondrial ATP synthesis to repair mitochondrial function. The effect is significantly enhanced. These results indicate that Angelica sinensis in the present invention has the effect of "improving mitochondrial respiratory function, oxidative phosphorylation efficiency and promoting mitochondrial ATP synthesis to repair mitochondrial function".

[0104] The experimental results in Table 3 suggest that compared with the product in Example 1, the relative abundance of harmful bacteria of Firmicutes in the component knock-out and knock-in party 7 product formed after knocking out small yellow ginger in the formula is significantly increased, and the relative abundance of beneficial bacteria of Bacteroidetes is significantly reduced; the content of indole and trimethylamine in the intestinal contents is also significantly increased. Although the expression rate of intestinal junction protein ZO-1 has not changed significantly, the content of indoxyl sulfate and trimethylamine oxide in serum has increased significantly due to the increased synthesis of indole and trimethylamine in the intestine, and the AI ​​value and P / L% have also been significantly increased. At the same time, TC and ROS levels are also significantly increased. However, the abnormal parameters of the component knock-out and knock-in party 7 products that reflect the weakening of the product efficacy are significantly reduced or increased relative to the product in Example 1. When small yellow ginger is knocked into the formula to increase its proportion, the component knock-out and knock-in party 8 products are obtained. Compared with the product in Example 1, these parameters are significantly better than the product in Example 1. These results suggest that compared with the product in Example 1, the knock-out and knock-in products of Fang 7 formed by removing the component of small yellow ginger from the formula have significantly weakened the effects of regulating the disorder of intestinal flora of mice induced by high fat, inhibiting the production of intestinal toxic metabolites indole and trimethylamine that damage mitochondrial function, preventing and treating atherosclerosis, and eliminating the high-fat and high-oxygen free radical microenvironment of mitochondrial damage. However, the knock-out and knock-in products of Fang 8 formed by adding small yellow ginger to the formula have significantly enhanced the effects of regulating the disorder of intestinal flora of mice induced by high fat, inhibiting the production of intestinal toxic metabolites indole and trimethylamine that damage mitochondrial function, preventing and treating atherosclerosis, and eliminating the high-fat and high-oxygen free radical microenvironment of mitochondrial damage. These results show that small yellow ginger has the effect of "regulating the disorder of intestinal flora of mice induced by high fat, inhibiting the production of intestinal toxic metabolites indole and trimethylamine that damage mitochondrial function, preventing and treating atherosclerosis, and eliminating the high-fat and high-oxygen free radical microenvironment of mitochondrial damage" in the present invention.

[0105] The experimental results of Table 3 show that compared with the product of Example 1, the relative abundance of harmful bacteria of Firmicutes in the component knock-out and knock-in square 9 products formed after knocking out the single-headed garlic in the prescription is significantly increased, and the relative abundance of beneficial bacteria of Bacteroidetes is significantly reduced; the content of indole and trimethylamine in the intestinal contents is also significantly increased; although the expression rate of intestinal junction protein ZO-1 has not changed significantly, the content of indoxyl sulfate and trimethylamine oxide in serum is also significantly increased due to the increased synthesis of indole and trimethylamine in the intestine; AI value and P / L% are also significantly increased. At the same time, its mitochondrial RCR, OPR and ATP content are also significantly decreased. However, the abnormal parameters of the component knock-out and knock-in square 9 products that reflect the weakening of the product efficacy are significantly reduced or increased relative to the product of Example 1. When the single-headed garlic is knocked into the prescription to increase its ratio, the component knock-out and knock-in square 10 products are obtained. Compared with the product of Example 1, these parameters are significantly better than the product of Example 1. These results suggest that compared with the product in Example 1, the knock-out and knock-in products of Fang 9 formed by removing the component formed by the single-headed garlic in the prescription have significantly weakened the effects of regulating the disorder of the intestinal flora of mice induced by high fat, inhibiting the production of intestinal toxic metabolites indole and trimethylamine that damage mitochondrial function, preventing and treating atherosclerosis, improving mitochondrial respiratory function and oxidative phosphorylation efficiency, and promoting mitochondrial ATP synthesis to repair mitochondrial function. However, the knock-out and knock-in products of Fang 10 formed by adding the component formed by the single-headed garlic in the prescription have significantly enhanced the effects of regulating the disorder of the intestinal flora of mice induced by high fat, inhibiting the production of intestinal toxic metabolites indole and trimethylamine that damage mitochondrial function, preventing and treating atherosclerosis, improving mitochondrial respiratory function and oxidative phosphorylation efficiency, and promoting mitochondrial ATP synthesis to repair mitochondrial function. These results show that the single-headed garlic has the effect of "regulating the disorder of the intestinal flora of mice induced by high fat, inhibiting the production of intestinal toxic metabolites indole and trimethylamine that damage mitochondrial function, preventing and treating atherosclerosis, improving mitochondrial respiratory function and oxidative phosphorylation efficiency, and promoting mitochondrial ATP synthesis to repair mitochondrial function".

[0106] The experimental results in Table 3 suggest that, compared with the product in Example 1, the AI ​​value and P / L% of the component knock-out and knock-in square 11 product formed after knocking out Antarctic krill oil in the formula are significantly increased. At the same time, TC and ROS levels are also significantly increased. However, the abnormal parameters of the component knock-out and knock-in square 11 products, which are significantly increased relative to the product in Example 1, reflect the weakening of the product efficacy. When the Antarctic krill oil is knocked into the formula to increase its proportion, the component knock-out and knock-in square 12 products are obtained. Compared with the product in Example 1, these parameters are significantly lower than those of the product in Example 1. These results suggest that, compared with the product in Example 1, the component knock-out and knock-in square 11 products formed by removing Antarctic krill oil from the formula have significantly weakened effects on preventing and treating atherosclerosis, eliminating the high-fat and high-oxygen free radical microenvironment of mitochondrial damage, etc. However, the component knock-out and knock-in square 12 products formed by adding Antarctic krill oil to the formula have significantly enhanced effects on preventing and treating atherosclerosis, eliminating the high-fat and high-oxygen free radical microenvironment of mitochondrial damage, etc. These results indicate that Antarctic krill oil in the present invention has the effect of "eliminating the high-fat and high-oxygen free radical microenvironment that damages mitochondria and preventing and treating atherosclerosis".

[0107] The experimental results in Table 3 show that compared with the product in Example 1, the relative abundance of harmful bacteria in the Firmicutes phylum was significantly increased, and the relative abundance of beneficial bacteria in the Bacteroidetes phylum was significantly reduced in the product formed by knocking out chitosan in the formula. The content of indole and trimethylamine in the intestinal contents was also significantly increased. Although the expression rate of the intestinal junction protein ZO-1 did not change significantly, the content of indoxyl sulfate and trimethylamine oxide in the serum was also significantly increased due to the increased synthesis of indole and trimethylamine in the intestine. The AI ​​value and P / L% were also significantly increased. At the same time, the sedimentation ratio of the product after dispersion in water was also significantly reduced. In addition, in order to evaluate its role in adsorbing toxic metabolites produced by abnormal intestinal flora that can induce mitochondrial damage and promoting the excretion of toxic metabolites, this experiment separately measured the contents of indole and trimethylamine in feces. The results showed that compared with the product of Example 1, the contents of indole and trimethylamine in the feces were opposite to those in the intestinal contents, and the contents of indole and trimethylamine were significantly reduced. However, the abnormal parameters reflecting the weakening of the efficacy and performance of the products in the knock-out and knock-in formula 13 products were significantly reduced or increased relative to the product of Example 1. When chitosan was additionally knocked into the formula to increase its proportion, the component knock-out and knock-in formula 14 products were obtained. Compared with the product of Example 1, these parameters were significantly better than those of the product of Example 1. These results suggest that compared with the product in Example 1, the knock-out and knock-in products of the formula 13, which remove the component formed by chitosan from the formula, have significantly weakened effects on regulating the disorder of the intestinal flora of mice induced by high fat, inhibiting the production of intestinal toxic metabolites indole and trimethylamine that damage mitochondrial function, adsorbing toxic metabolites indole and trimethylamine and promoting their efflux, preventing and treating atherosclerosis, and increasing the stability of the water-dispersible suspension of the product. However, the knock-out and knock-in products of the formula 14, which add chitosan to the formula, have significantly enhanced effects on regulating the disorder of the intestinal flora of mice induced by high fat, inhibiting the production of intestinal toxic metabolites indole and trimethylamine that damage mitochondrial function, adsorbing toxic metabolites indole and trimethylamine and promoting their efflux, preventing and treating atherosclerosis, and increasing the stability of the water-dispersible suspension of the product. These results indicate that chitosan in the present invention has the effects of "regulating the disorder of intestinal flora of mice induced by high fat, inhibiting the production of intestinal toxic metabolites indole and trimethylamine that damage mitochondrial function, adsorbing toxic metabolites indole and trimethylamine and promoting their excretion, preventing and treating atherosclerosis, and increasing the stability of the product's water-dispersible suspension."

[0108] It can be seen from the experimental results in Table 3 that, compared with the product in Example 1, the expression rate of intestinal junction protein ZO-1 in the product 15 formed by knocking out the attapulgite in the formula was significantly reduced, and the contents of indoxyl sulfate and trimethylamine oxide in serum were also significantly increased; the AI ​​value and P / L% were also significantly increased. At the same time, the sedimentation ratio of the product after dispersion in water was also significantly reduced, and obvious free oil was visible. The powdered product showed oily softening. In the oil absorbing paper adsorption test, the oil absorbing paper was obviously infiltrated. In addition, in order to evaluate its effect of adsorbing toxic metabolites that can induce mitochondrial damage produced by abnormal intestinal flora and promoting the excretion of toxic metabolites, this experiment separately measured the content of indole and trimethylamine in feces. The results showed that compared with the product of Example 1, the content of indole and trimethylamine produced in feces was significantly reduced. However, the abnormal parameters reflecting the weakening of product efficacy and performance were significantly reduced or increased in the product of component knock-out and knock-in formula 15 relative to the product of Example 1. When the proportion of attapulgite was increased by knocking in the formula, the product of component knock-out and knock-in formula 16 was obtained. Compared with the product of Example 1, these parameters were significantly better than those of the product of Example 1. These results suggest that compared with the product of Example 1, the product of component knock-out and knock-in formula 15 formed by removing attapulgite from the formula has significantly weakened effects on enhancing intestinal barrier function, reducing the absorption of intestinal toxic metabolites that damage mitochondrial function, adsorbing toxic metabolites indole and trimethylamine and promoting their excretion, preventing and treating atherosclerosis, achieving solidification of liquid components of Antarctic krill oil, and increasing the stability of water-dispersed suspension of products. However, the addition of attapulgite to the formula to form the components of knock-out and knock-in formula 16 products has significantly enhanced the effects of enhancing the intestinal barrier function, reducing the absorption of intestinal toxic metabolites that damage mitochondrial function, adsorbing toxic metabolites indole and trimethylamine and promoting their efflux, preventing and treating atherosclerosis, solidifying the liquid components of Antarctic krill oil, and increasing the stability of the water-dispersible suspension of the product. These results show that attapulgite in the present invention has the effects of "enhancing the intestinal barrier function, reducing the absorption of intestinal toxic metabolites that damage mitochondrial function, adsorbing toxic metabolites indole and trimethylamine and promoting their efflux, preventing and treating atherosclerosis, solidifying the liquid components of Antarctic krill oil, and increasing the stability of the water-dispersible suspension of the product".

[0109] The experimental results of Table 3 found that compared with the product of Example 1, the component knock-out and knock-in square 17 products formed after knocking out stevia leaves in the prescription had a significantly reduced taste score, however, the component knock-out and knock-in square 17 products were significantly reduced in taste score relative to the product of Example 1, and when stevia leaves were additionally knocked into the prescription to increase its ratio, the component knock-out and knock-in square 18 products were obtained, and compared with the product of Example 1, their taste scores were significantly increased. These results suggest that compared with the product of Example 1, the component knock-out and knock-in square 17 products formed by removing stevia leaves from the prescription, the effect of improving the taste of the product was significantly weakened. However, the component knock-out and knock-in square 18 products formed by adding stevia leaves to the prescription, the effect of improving the taste of the product was significantly enhanced. These results show that stevia leaves have the effect of improving the taste of the product in the present invention.

[0110] In summary, by knocking out and knocking in each component in the formula, it was confirmed that each component in the formula played its own beneficial role.

[0111] (4) Verification of the synergistic effect of Dendrobium officinale, Astragalus, Ginger, Garlic, and Chitosan in the formula of the present invention in regulating intestinal flora disorders and inhibiting the production of intestinal toxic metabolites that damage mitochondrial function Example 1 is used as a control. According to the same formula ratio as in Example 1, the Dendrobium officinale, Astragalus, Small Yellow Turmeric, Garlic, and Chitosan in the formula are knocked out to different degrees to form the following component knocking formula product: Component knock-out formula 4-1 product formula: by mass, 30 parts of astragalus, 6 parts of angelica, 10 parts of small yellow turmeric, 10 parts of single-headed garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out recipe 4-2 product formula: by mass, 6 parts of angelica, 10 parts of small yellow ginger, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out recipe 4-3 product formula: by mass, 6 parts of angelica, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out formula 4-4 product formula: by mass, 6 parts of angelica, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out formula 4-5 product formula: by mass, 6 parts of angelica, 10 parts of Antarctic krill oil, 5 parts of attapulgite, and 3 parts of stevia.

[0112] 60 SPF-grade ApoE - / - The mice were fed with normal feed in the animal quarantine room for 1 week. If there were no abnormalities after 1 week, they were transferred to the feeding room for group intervention. - / -The mice were randomly divided into 6 groups, namely, Example 1 group, component knockout prescription 4-1 group to component knockout prescription 4-5 group, 10 mice in each group, half male and half female. Each group was fed with a high-fat diet (21% fat, 0.25% cholesterol, 1% choline, 77.75% basic feed), and free access to food and water. At the same time, each group was intragastrically administered with a physiological saline solution of the product of Example 1 and the products of component knockout prescription 4-1 to component knockout prescription 4-5 at a rate of 0.1 mL / 10 g (according to the set dosage for administration, the product of Example 1 and the products of component knockout prescription 4-1 to component knockout prescription 4-5 were dissolved in physiological saline). The intervention lasted for 12 weeks. 24 hours after the last administration, the mice were anesthetized and killed by dislocating the cervical vertebra. The intestinal contents were quickly dissected and obtained. The composition of the intestinal flora was analyzed by 16S rDNA sequencing technology, and the content of indole and trimethylamine in the intestinal contents was determined by ultra-high performance liquid chromatography. The effects of different components of the knockout formula products on regulating the intestinal flora of atherosclerosis model mice and inhibiting the production of intestinal toxic metabolites that damage mitochondrial function were compared and analyzed. The results are shown in Table 4.

[0113]

[0114] Note: Superscript letters a~f indicate significant differences between two groups.

[0115] It can be seen from the experimental results in Table 4 that from Example 1 of the whole recipe, after knocking out Dendrobium officinale, Astragalus, Small Yellow Turmeric, Garlic and chitosan in turn, the component knocked-out recipe 4-1 to the component knocked-out recipe 4-5 products formed, not only the relative abundance of harmful bacteria of the Firmicutes phylum showed a significant increase step by step (each component knocked out was counted as one level), while the relative abundance of beneficial bacteria of the Bacteroidetes phylum showed a significant decrease step by step, and at the same time, the content of indole and trimethylamine in the intestinal contents also showed a significant increase step by step; and the trend of increase and decrease became more and more significantly intensified with the increase of the knocked-out components. These results indicate that, as the Dendrobium officinale, Astragalus, Turmeric, Allium sativum, and chitosan in Example 1 were knocked out in sequence, their effects on regulating intestinal flora disorders and inhibiting the production of enteric toxic metabolites that damage mitochondrial function showed a significant decrease in a step-by-step manner, thereby confirming that the Dendrobium officinale, Astragalus, Turmeric, Allium sativum, and chitosan in the formula of the present invention have a synergistic effect in regulating intestinal flora disorders and inhibiting the production of enteric toxic metabolites that damage mitochondrial function.

[0116] (5) Verification of the synergistic effect of Dendrobium officinale, Astragalus membranaceus, and Attapulgite in the formula of the present invention in enhancing the intestinal barrier function and reducing the absorption of intestinal toxic metabolites that damage mitochondrial function Example 1 is used as a control. According to the same formula ratio as in Example 1, the Dendrobium officinale, Astragalus, and Attapulgite in the formula are knocked out to different degrees to form the following component knocked-out formula product: Component knock-out recipe 5-1 product formula: by mass, 30 parts of astragalus, 6 parts of angelica, 10 parts of small yellow turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out recipe 5-2 product formula: by mass, 6 parts of angelica, 10 parts of small yellow ginger, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out recipe 5-3 product formula: by mass, 6 parts of angelica, 10 parts of turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, and 3 parts of stevia.

[0117] 40 SPF-grade ApoE - / - The mice were fed with normal feed in the animal quarantine room for 1 week. If there were no abnormalities after 1 week, they were transferred to the feeding room for group intervention. - / - The mice were randomly divided into 4 groups, namely, Example 1 group, component knockout prescription 5-1 group to component knockout prescription 5-3 group, 10 mice in each group, half male and half female. Each group was fed with a high-fat diet (21% fat, 0.25% cholesterol, 1% choline, 77.75% basic feed), and free access to food and water. At the same time, each group was gavaged with a physiological saline solution of the product of Example 1 and the products of component knockout prescription 5-1 to component knockout prescription 5-3 at 0.1 mL / 10 g (according to the set dosage for administration, the product of Example 1 and the products of component knockout prescription 5-1 to component knockout prescription 5-3 were dissolved in physiological saline). The intervention was continued for 12 weeks. 24 hours after the last administration, the eyeballs were removed after anesthesia to collect blood, and then the mice were killed by cervical dislocation. The colon was quickly dissected. The expression of intestinal junction protein ZO-1 was determined by immunohistochemical staining, and the contents of indoxyl sulfate and trimethylamine oxide in serum were determined by ultra-high performance liquid chromatography. The differences in the effects of different components of the knockout formula products on enhancing intestinal barrier function and reducing the absorption of intestinal toxic metabolites that damage mitochondrial function were compared and analyzed. The results are shown in Table 5.

[0118]

[0119] Note: Different letters in superscript a~d indicate significant differences between two groups.

[0120] It can be seen from the experimental results of Table 5 that from Example 1 of the whole recipe, after knocking out Dendrobium officinale, Astragalus membranaceus, and Attapulgite in sequence, the products of component knocking out recipe 5-1 to component knocking out recipe 5-3 formed not only showed a significant decrease in the expression rate of intestinal junction protein ZO-1, but also showed a significant increase in the content of indoxyl sulfate and trimethylamine oxide in serum; and the trend of increase and decrease became more and more significant as the number of knocked out components increased. These results show that with the knocking out of Dendrobium officinale, Astragalus membranaceus, and Attapulgite in Example 1, the effect of enhancing the intestinal barrier function and reducing the absorption of intestinal toxic metabolites that damage mitochondrial function showed a significant decrease in step by step, thereby confirming that Dendrobium officinale, Astragalus membranaceus, and Attapulgite in the formula of the present invention have a synergistic effect in enhancing the intestinal barrier function and reducing the absorption of intestinal toxic metabolites that damage mitochondrial function.

[0121] (6) Verification of the synergistic effect of chitosan and attapulgite in the formula of the present invention in absorbing toxic metabolites produced by abnormal intestinal flora that can induce mitochondrial damage and promoting the excretion of toxic metabolites Example 1 was used as a control. According to the same formula ratio as in Example 1, the chitosan and attapulgite in the formula were knocked out to different degrees to form the following component knocked-out products: Component knock-out recipe 6-1 product formula: by mass, 40 parts of Dendrobium officinale, 30 parts of Astragalus, 6 parts of Angelica sinensis, 10 parts of small yellow turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out recipe 6-2 product formula: by mass, 40 parts of Dendrobium officinale, 30 parts of Astragalus, 6 parts of Angelica sinensis, 10 parts of small yellow turmeric, 10 parts of single-headed garlic, 10 parts of Antarctic krill oil, and 3 parts of Stevia.

[0122] 30 SPF-grade ApoE - / - The mice were fed with normal feed in the animal quarantine room for 1 week. If there were no abnormalities after 1 week, they were transferred to the feeding room for group intervention. - / -The mice were randomly divided into three groups, namely, Example 1 group, component knockout 6-1 group and component knockout 6-2 group, with 10 mice in each group, half male and half female. Each group was fed with a high-fat diet (21% fat, 0.25% cholesterol, 1% choline, 77.75% basic feed) and free access to food and water. At the same time, each group was intragastrically administered with 0.1 mL / 10 g of a physiological saline solution of the product of Example 1 and the component knockout 6-1 and component knockout 6-2 products (according to the set dosage for administration, the product of Example 1 and the component knockout 6-1 and component knockout 6-2 products were dissolved in physiological saline). The intervention lasted for 12 weeks. 24 hours after the last administration, the feces of mice in each group were collected with a metabolic cage, and the contents of indoxyl and trimethylamine in the feces were determined by ultra-high performance liquid chromatography. The differences in the adsorption of toxic metabolites produced by abnormal intestinal flora that can induce mitochondrial damage and promote the excretion of toxic metabolites by different component knockout products were compared and analyzed. The results are shown in Table 6.

[0123]

[0124] Note: Superscript letters a~c indicate significant differences between two groups.

[0125] It can be seen from the experimental results of Table 6 that from Example 1 of the whole recipe, after knocking out chitosan and attapulgite in sequence, the components of knocked-out recipe 6-1 and knocked-out recipe 6-2 formed, not only the content of indole and trimethylamine in feces showed a significant decrease step by step, but also the trend of its decrease became more and more significant as the number of knocked-out components increased. These results show that with the sequential knocking out of chitosan and attapulgite in the recipe in Example 1, the effect of adsorbing toxic metabolites that can induce mitochondrial damage produced by abnormal intestinal flora and promoting the efflux of toxic metabolites showed a significant decrease step by step, thereby confirming that chitosan and attapulgite in the formula of the present invention have a synergistic effect in adsorbing toxic metabolites that can induce mitochondrial damage produced by abnormal intestinal flora and promoting the efflux of toxic metabolites.

[0126] (7) Verification of the synergistic effect of astragalus, angelica and garlic in the formula of the present invention on improving mitochondrial respiratory function, oxidative phosphorylation efficiency and promoting mitochondrial ATP synthesis Example 1 was used as a control. According to the same formula ratio as in Example 1, the astragalus, angelica, and garlic in the formula were knocked out to different degrees to form the following component knocked-out formula product: Component knock-out recipe 7-1 product formula: by mass, 40 parts of Dendrobium officinale, 6 parts of Angelica sinensis, 10 parts of small yellow turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out recipe 7-2 product formula: by mass, 40 parts of Dendrobium officinale, 10 parts of small yellow ginger, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out recipe 7-3 product formula: by mass, 40 parts of Dendrobium officinale, 10 parts of small yellow turmeric, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia.

[0127] 40 SPF-grade ApoE - / - The mice were fed with normal feed in the animal quarantine room for 1 week. If there were no abnormalities after 1 week, they were transferred to the feeding room for group intervention. - / - The mice were randomly divided into 4 groups, namely, Example 1 group, component knockout prescription 7-1 group to component knockout prescription 7-3 group, 10 mice in each group, half male and half female. Each group was fed with a high-fat diet (21% fat, 0.25% cholesterol, 1% choline, 77.75% basic feed), and free access to food and water. At the same time, each group was gavaged with 0.1 mL / 10 g of physiological saline solution of the product of Example 1 and the products of component knockout prescription 7-1 to component knockout prescription 7-3 (according to the set dosage for administration, the product of Example 1 and the products of component knockout prescription 7-1 to component knockout prescription 7-3 were dissolved in physiological saline). The intervention was continued for 12 weeks. 24 hours after the last administration, the mice were killed by cervical dislocation after anesthesia. The thoracic aorta was quickly dissected. The mitochondria of the thoracic aorta were isolated by Clark method, and the RCR, OPR and ATP contents of the mitochondria of the thoracic aorta of each group were measured and evaluated. Comparative analysis of the differences in improving mitochondrial respiratory function, improving oxidative phosphorylation efficiency and promoting mitochondrial ATP synthesis of different component knockout prescription products. The results are shown in Table 7.

[0128]

[0129] Note: Different letters in superscript a~d indicate significant differences between two groups.

[0130] As shown in the experimental results of Table 7, from the embodiment 1 of the whole party, after knocking out astragalus, angelica, and garlic, the components knocking out party 7-1 to the components knocking out party 7-3 products formed, not only the RCR, OPR and ATP content of the thoracic aorta mitochondria all show a significant decrease step by step, but also the trend of its decline is more and more significant as the number of knocked out components increases. These results show that as the astragalus, angelica, and garlic in the square in Example 1 are knocked out successively, the effect of improving mitochondrial respiratory function, oxidative phosphorylation efficiency, and promoting mitochondrial ATP synthesis shows a significant decrease step by step, thereby confirming that the astragalus, angelica, and garlic in the formula of the present invention have a synergistic effect in improving mitochondrial respiratory function, oxidative phosphorylation efficiency, and promoting mitochondrial ATP synthesis.

[0131] (8) Verification of the synergistic effect of Dendrobium candidum, Astragalus, Ginger, and Antarctic krill oil in the formula of the present invention in inhibiting the generation of reactive oxygen species and lipid deposition and eliminating the microenvironment of high fat and high oxygen free radicals that damage mitochondria Example 1 was used as a control. According to the same formula ratio as in Example 1, the Dendrobium officinale, Astragalus, Turmeric, and Antarctic krill oil in the formula were knocked out to different degrees to form the following component knocked-out formula product: Components: Product formula of knockout formula 8-1: by mass, 30 parts of astragalus, 6 parts of angelica, 10 parts of small yellow turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out formula 8-2 product formula: by mass, 6 parts of angelica, 10 parts of small yellow ginger, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Components: The product formula of knockout formula 8-3: by mass, 6 parts of angelica, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Component knock-out recipe 8-4 product formula: by mass, 6 parts of angelica, 10 parts of garlic, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia.

[0132] 50 SPF-grade ApoE - / - The mice were fed with normal feed in the animal quarantine room for 1 week. If there were no abnormalities after 1 week, they were transferred to the feeding room for group intervention. - / - The mice were randomly divided into 5 groups, namely, Example 1 group, component knockout 8-1 group to component knockout 8-4 group, 10 mice in each group, half male and half female. Each group was fed with a high-fat diet (21% fat, 0.25% cholesterol, 1% choline, 77.75% basic feed), and free access to food and water. At the same time, each group was gavaged with 0.1 mL / 10 g of physiological saline solution of the product of Example 1 and the products of component knockout 8-1 to component knockout 8-4 (according to the set dosage for administration, the product of Example 1 and the products of component knockout 8-1 to component knockout 8-4 were dissolved in physiological saline). The intervention lasted for 12 weeks. After 24 hours of the last administration, the intervention lasted for 12 weeks. 24 hours after the last administration, the eyeballs were removed after anesthesia to collect blood, and then the mice were killed by dislocating the cervical vertebra. The thoracic aorta was quickly dissected. The thoracic aorta was quickly dissected. The mitochondria of the thoracic aorta were isolated by Clark method. The TC content in serum was analyzed using a kit, and the ROS level in the mitochondria of the thoracic aorta was determined using the MitoSOXTM Red probe fluorescence staining method. The differences in the elimination of the high-fat and high-oxygen free radical microenvironment of mitochondrial damage by the knockout formula products with different components were compared and analyzed. The results are shown in Table 8.

[0133] It can be seen from the experimental results of Table 8 that from Example 1 of the whole recipe, after knocking out Dendrobium officinale, Astragalus, Turmeric, and Antarctic krill oil in sequence, the components knocked out recipe 8-1 to the components knocked out recipe 8-4 products formed, not only the serum TC and thoracic aorta mitochondrial ROS showed a significant increase step by step, but also the trend of increase became more and more significant as the number of knocked out components increased. These results show that with the sequential knocking out of Dendrobium officinale, Astragalus, Turmeric, and Antarctic krill oil in the recipe of Example 1, the effect of eliminating the high-fat and high-oxygen free radical microenvironment of mitochondrial damage showed a significant weakening step by step, thereby confirming that Dendrobium officinale, Astragalus, Turmeric, and Antarctic krill oil in the formula of the present invention have a synergistic effect in eliminating the high-fat and high-oxygen free radical microenvironment of mitochondrial damage.

[0134]

[0135] Note: Superscript letters a~e indicate significant differences between two groups.

[0136] (9) Verification of the synergistic effect of Dendrobium officinale, chitosan and attapulgite in the formula of the present invention on enhancing the stability of solid beverage dispersion in water Example 1 was used as a control. According to the same formula ratio as in Example 1, the Dendrobium officinale, chitosan, and attapulgite in the formula were knocked out to different degrees to form the following component knocked-out product: Components: Product formula of knockout formula 9-1: by mass, 30 parts of astragalus, 6 parts of angelica, 10 parts of small yellow turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 3 parts of chitosan, 5 parts of attapulgite, and 3 parts of stevia; Components: The product formula of formula 9-2: by mass, 30 parts of astragalus, 6 parts of angelica, 10 parts of small yellow turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, 5 parts of attapulgite, and 3 parts of stevia; The components of the product formula 9-3 are as follows: by mass: 30 parts of astragalus, 6 parts of angelica, 10 parts of turmeric, 10 parts of garlic, 10 parts of Antarctic krill oil, and 3 parts of stevia.

[0137] 2 g of the product of Example 1 and the products of components knocked out from formula 9-1 to 9-3 were placed in a 25 mL stoppered measuring cylinder, 15 mL of deionized water was added, and the mixture was shaken for 5 minutes and then allowed to stand. 3h ) The height of the suspension after the sedimentation ratio = H 3h / H0 formula was used to calculate and compare the sedimentation ratio differences of each product to evaluate the stability of the solid beverages dispersed in water of the knockout products with different components. Among them, the maximum sedimentation ratio is 1, and the smaller the sedimentation ratio, the worse the stability of the product after dispersion in water. The results are shown in Table 9.

[0138]

[0139] Note: Different letters in superscript a~d indicate significant differences between two groups.

[0140] It can be seen from the experimental results of Table 9 that from the embodiment 1 of the whole recipe, after knocking out Dendrobium officinale, chitosan, and attapulgite in sequence, the formed component knocking out recipe 9-1 to the component knocking out recipe 9-3 products not only show a significant decrease in sedimentation ratio step by step, but also the downward trend becomes more and more significant as the number of knocked out components increases. These results show that as Dendrobium officinale, chitosan, and attapulgite are knocked out in sequence in the recipe of embodiment 1, the effect of enhancing the stability of dispersion in solid beverage water shows a significant decrease step by step, thereby confirming that Dendrobium officinale, chitosan, and attapulgite in the formula of the present invention have a synergistic effect in enhancing the stability of dispersion in solid beverage water.

[0141] (10) Survey on users’ acceptance of the dosage form of the product of the present invention The product of Example 1 was packed into capsules and pressed into tablets. 50 volunteers of different ages and genders were randomly selected to conduct a questionnaire survey on their willingness to choose the product of Example 1, capsules and tablets made from the product of Example 1. The results showed that the selection rate of volunteers for the product of Example 1 was 96%, the selection rate for tablets was 4%, and the selection rate for capsules was 0%. This shows that the patented formula product makes the product dosage form into a daily food in the form of a solid beverage, which can greatly improve the compliance of users.

[0142] The above embodiments are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with the technology to understand the content of the present invention and implement it accordingly, and they cannot be used to limit the protection scope of the present invention. Any equivalent transformation or modification made according to the spirit of the present invention should be included in the protection scope of the present invention.

Claims

1. A solid beverage for preventing and treating atherosclerosis, characterized in that: The invention is prepared from the following raw material components in parts by mass: 40-60 parts of dendrobium officinale, 30-50 parts of astragalus, 6-10 parts of angelica, 10-15 parts of turmeric, 10-15 parts of garlic, 10-20 parts of Antarctic krill oil, 3-6 parts of chitosan, 5-10 parts of attapulgite and 3-6 parts of stevia.

2. The solid beverage for preventing and treating atherosclerosis according to claim 1, characterized in that: The Dendrobium officinale is three-year-old medicinal-grade Dendrobium officinale powder that is crushed and sieved through 80-120 meshes.

3. The solid beverage for preventing and treating atherosclerosis according to claim 1, characterized in that: The astragalus is astragalus powder obtained by crushing the dried roots of the leguminous plants Astragalus mongolica or Astragalus membranaceus and passing through a 60-mesh sieve.

4. The solid beverage for preventing and treating atherosclerosis according to claim 1, characterized in that: The angelica powder is the dried root of the umbelliferae plant angelica which is crushed and passed through a 60-mesh sieve.

5. The solid beverage for preventing and treating atherosclerosis according to claim 1, characterized in that: The small yellow turmeric is the fresh tuber of the small yellow turmeric plant of the ginger family.

6. The solid beverage for preventing and treating atherosclerosis according to claim 1, characterized in that: The single-headed garlic is the dried bulb of the single-headed garlic of the Liliaceae family.

7. The solid beverage for preventing and treating atherosclerosis according to claim 1, characterized in that: The chitosan is a pharmaceutical grade chitosan powder with a molecular weight of 50-90 kDa and a sieve passing through 80-120 meshes.

8. The solid beverage for preventing and treating atherosclerosis according to claim 1, characterized in that: The attapulgite is a medicinal adsorption grade attapulgite powder that has passed through a 80-120 mesh sieve.

9. The solid beverage for preventing and treating atherosclerosis according to claim 1, characterized in that: The solid beverage has a total saponin content of ≥100 μg / g, a ferulic acid content of ≥20 μg / g, a total flavonoid content of ≥30 mg / g, a total polysaccharide content of ≥250 mg / g, and a volatile oil content of ≥10 mg / g. After being dispersed in water, the sedimentation ratio is ≥0.92 within 3 hours.

10. A method for preparing a solid beverage for preventing and treating atherosclerosis, characterized in that: The following steps are involved: The weight parts of the raw materials of the solid beverage for preventing and treating atherosclerosis according to any one of claims 1 to 9, (1) After the astragalus and angelica are crushed to pass through a 60-mesh sieve, the astragalus and angelica powders are weighed, fully mixed and placed in an extraction device, deionized water is added, soaked, refluxed and decocted under normal pressure, and filtered; the filter residue is decocted again under the same conditions and filtered; the two filtrates are combined and concentrated under reduced pressure to a relative density of 0.4 to 0.8 under the conditions of a vacuum degree of 0.090 to 0.098 MPa and a temperature of 60 to 80 degrees Celsius to obtain a mixed extract of astragalus and angelica; (2) Wash and drain the turmeric, peel it and cut it into dices; (3) Peel the garlic cloves, remove impurities, wash and drain; (4) placing the pre-treated raw materials of turmeric and garlic obtained in steps (2) and (3) in a homogenizer, adding the mixed extract of astragalus and angelica obtained in step (1), and fully homogenizing with the homogenizer until the slurry passes through a 80-120 mesh sieve; (5) spraying the Antarctic krill oil evenly on the attapulgite powder, mixing thoroughly, and sealing and placing for 6 to 9 hours to obtain a mixture of the Antarctic krill oil and the attapulgite; (6) adding chitosan powder to the homogenate obtained in step (4), stirring and suspending the chitosan powder, and then evenly adding the chitosan powder to the mixture of Antarctic krill oil and attapulgite obtained in step (5) by passing the mixture through a 80-120 mesh sieve, and stirring and mixing the mixture evenly; (7) Grinding Dendrobium officinale and Stevia rebaudiana respectively until they pass through a 80-120 mesh sieve, and mixing the two uniformly by an equal amount increasing method to obtain a mixed powder of Dendrobium officinale and Stevia rebaudiana; (8) The mixture obtained in step (6) is added to the mixed powder of Dendrobium officinale and Stevia in equal amounts, and after being fully mixed, the mixture is passed through a 80-120 mesh sieve to obtain a solid beverage for preventing and treating atherosclerosis.

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