Composition containing bioactive peptide and application of composition in preparation of eye care essence

Abstract

The invention provides a composition containing bioactive peptide, which is prepared from the following components in parts by weight: 3 to 6 parts of bioactive peptide, 0.2 to 0.6 part of hyaluronic acid, 1 to 3 parts of witch hazel, 0.1 to 0.5 part of arbutin and 100 to 150 parts of water. The feed also comprises 6-12 parts of a plant extract mixed solution and 0.5-1.5 parts of a vitamin mixture; the bioactive peptide is a bioactive peptide extracted from fish; the plant extraction mixed solution is a plant extraction solution of a mixture of aloe, grape seeds, cassia seeds and mint in a mass ratio of 1: (2-3): (1.5-2.5): (3-4); under the synergistic effect of all the components, the wrinkle removal, skin care and other properties of the composition can be obviously improved, and when the composition is applied to eye care essence, the eye skin care requirements can be better met.

Description

Technical Field

[0001] The invention belongs to the technical field of preparation of care products, and specifically relates to a composition containing bioactive peptides and application of the composition in preparing eye care essence. Background Art

[0002] The skin around the eyes is the most vulnerable part of the human body and is also the first place where the body ages. The skin around the eyes is directly related to opening, closing and moving our eyes. The thickness of the skin is only one-tenth of that of ordinary skin, and there are fewer sebaceous glands and sweat glands under the skin, so the skin is relatively thin and loose, but more elastic. As we age, the collagen and elastic fibers in the skin gradually decrease, leading to the appearance of fine lines and wrinkles; poor blood circulation, pigmentation or genetic factors cause dark circles; water retention or poor lymphatic circulation causes eye bags and edema.

[0003] Existing eye care methods can be divided into chemical methods and physical methods according to their mode of action. Chemical methods mainly refer to the use of eye drops made of medicines containing various artificial or natural chemical ingredients to directly drip into the eyes to act on the eyeballs, so that people can relieve visual fatigue after long-term use of the eyes. However, this method is easy to damage the retina of the eye and has great side effects if used for a long time. Physical methods mainly refer to the use of various physical therapy equipment to massage the eye contour, thereby relieving the appearance of various eye symptoms. Because the skin around the eyes is very delicate and thin, about one-third to one-fifth the thickness of the skin on other parts of the face, it is very susceptible to the stimulation and influence of the external environment and the pressure inside the body. Long-term direct action of physical therapy equipment on the eye contour also has certain safety hazards, which can easily cause secondary damage to the eyes.

[0004] Patent CN113197815A proposes an eye essence cream and an eye care method, which includes the following raw materials by weight: moisturizer: 5-10 parts; emulsifier: 19-30 parts; preservative: 0.4-1 parts; active agent: 1.21-5.66 parts; the active agent includes aloe extract, Centella asiatica extract, bifida fermentation product lysate and adenosine; it has little irritation to the skin around the eyes and can reduce wrinkles and improve elasticity to a certain extent. However, the existing eye care essence needs to be further improved in terms of skin care and wrinkle removal. Summary of the invention

[0005] The purpose of the present invention is to provide a composition containing bioactive peptides. By adding different active components, under the synergistic effect of each component, the wrinkle removal, skin care and other care properties of the composition are significantly improved. When it is applied to eye care essence, it can better meet the care needs of the skin around the eyes.

[0006] In order to achieve the above object, the present invention provides the following technical solutions: A composition containing bioactive peptides, comprising the following components in parts by weight: 3-6 parts of bioactive peptides, 0.2-0.6 parts of hyaluronic acid, 1-3 parts of witch hazel, 0.1-0.5 parts of arbutin and 100-150 parts of water; A method for preparing a composition containing bioactive peptides comprises the following steps: S1: Extraction of bioactive peptides; S2: preparing a plant extract mixture; S3: Mix 3-6 parts of bioactive peptides, 1-3 parts of witch hazel, 0.1-0.5 parts of arbutin and 100-150 parts of water, heat to 30-40°C, and stir evenly; S4: adding 6-12 parts of the plant extract mixture and 0.5-1.5 parts of the vitamin mixture, heating to 45-55° C., stirring for 10-20 minutes, and then ultrasonically dispersing for 30-50 minutes to obtain a composition containing bioactive peptides.

[0007] Preferably, step S1 is specifically as follows: boiling the pre-treated clean fish skin at 90°C for 3h, filtering with 250-350 mesh gauze to obtain a collagen aqueous solution; adding 1-3 parts of alkaline protease, 0.8-1.5 parts of neutral protease and 2-3 parts of papain to 30-50 parts of the collagen aqueous solution, and performing enzymolysis at 60°C for 3h; inactivating the enzyme at 80°C for 40min, and filtering to obtain an enzymolysis solution; and using an ultrafiltration membrane package with a molecular weight cutoff of 3-5KDa for interception to obtain bioactive peptides.

[0008] Preferably, the composition further comprises 6-12 parts of a plant extract mixture.

[0009] Preferably, the composition further comprises 0.5-1.5 parts of a vitamin mixture.

[0010] Preferably, the bioactive peptides are bioactive peptides extracted from fish.

[0011] Preferably, the plant extract mixture is a plant extract mixture of aloe vera, grape seed, cassia seed and mint in a mass ratio of 1:2-3:1.5-2.5:3-4.

[0012] Preferably, the vitamin mixture is vitamin E, vitamin C and vitamin D in a mass ratio of 1:1-2:1-3.

[0013] Preferably, step S2 specifically comprises: mixing and crushing aloe vera, grape seeds, cassia seeds and mint in a mass ratio of 1:2-3:1.5-2.5:3-4; adding into 10-20 times by weight of 70% ethanol solution, and ultrasonically extracting for 45 minutes to obtain a plant mixed extract.

[0014] The invention discloses an application of a composition containing bioactive peptides in preparing eye care essence.

[0015] Preferably, 15-25 parts of the composition, 0.5-1.5 parts of a moisturizer (pentylene glycol), 0.4-0.8 parts of an emulsifier (castor oil polyoxyethylene ether-80), 1-2 parts of a thickener (guar gum) and 0.2-0.6 parts of hyaluronic acid are mixed, stirred evenly at a temperature of 45-55° C., and the eye care essence can be obtained after sterilization.

[0016] Bioactive peptides are a class of small molecule protein fragments with specific physiological functions, usually composed of 2 to 20 amino acids. They are widely present in nature and can be extracted from food, plants, animals and microorganisms or manufactured through synthetic technology. Bioactive peptides have shown important value in many fields such as medicine, cosmetics, food and agriculture due to their diverse functions and applications. Bioactive peptides can exhibit a variety of physiological functions, including antioxidant, antibacterial, anti-inflammatory, blood pressure lowering, immune response regulation, and wound healing promotion. These functions give them a wide range of potential in different application scenarios. Due to the particularity of their structure, bioactive peptides can bind to specific receptors or targets, exert precise effects, and reduce the occurrence of side effects. Many bioactive peptides are derived from natural substances, so they usually have high biocompatibility and low toxicity, and are suitable for use in humans and animals. Through chemical synthesis or genetic engineering technology, bioactive peptides can be structurally modified to enhance their stability and functionality, or to extend their half-life.

[0017] Witch hazel, also known as willow herb or witch hazel, is a deciduous shrub or small tree native to North America. It belongs to the family Hamamelidaceae, with the most famous species being the witch hazel. Witch hazel is valued for its medicinal, ornamental, and industrial uses. Witch hazel usually blooms in the fall, with yellow or orange-red flowers and a unique four-petal structure that resembles a ribbon, hence the name "witch hazel". Its leaves are oval with serrated edges and turn bright yellow or red in the fall. The bark and leaves of witch hazel are rich in tannins and other active ingredients that give it a variety of medicinal and skin care benefits. Witch hazel extract has natural antibacterial and antioxidant properties that can inhibit bacterial growth and prevent skin infections while neutralizing free radicals and delaying skin aging. Relieves Varicose Veins and Hemorrhoids: Witch hazel's astringent and blood vessel wall-strengthening effects make it one of the traditional herbal remedies for varicose veins and hemorrhoids. It can help improve blood circulation and reduce venous dilation and inflammation.

[0018] Arbutin, also known as bearberry extract, is a natural compound extracted from bearberry, and can also be obtained from other plants such as pear bark, wheat and pearl berry. Arbutin is a β-D-glucoside that contains parahydroxyphenol in its molecule. It can be converted into hydroquinone by enzymatic or acidic hydrolysis, which is the key component of its whitening effect. The main function of arbutin is to reduce the production of melanin by inhibiting the activity of tyrosinase. Tyrosinase is a key enzyme in the synthesis of melanin, responsible for converting tyrosine into dopa, which in turn forms melanin. Arbutin reduces the production of melanin by binding to tyrosinase and preventing it from catalyzing the reaction. Compared with the direct use of hydroquinone, arbutin has a milder effect and fewer side effects. It needs to be gradually metabolized into hydroquinone in the skin, so it does not produce a strong whitening effect immediately, but long-term use can effectively lighten spots and brighten the skin. Arbutin also has certain antioxidant properties, which can help neutralize free radicals, delay skin aging, and protect the skin from damage by ultraviolet rays and other environmental factors. Arbutin has mild anti-inflammatory and antibacterial properties, which can help soothe skin inflammation and reduce the occurrence of acne. For this reason, it is often used in skin care products to treat acne scars and hyperpigmentation.

[0019] Aloe vera is a succulent plant whose leaves are rich in a gelatinous substance called aloe vera gel; multiple active ingredients: aloe vera gel is rich in vitamins (such as vitamin A, C, E), minerals (such as calcium, magnesium, zinc), amino acids, polysaccharides and anthraquinone compounds.

[0020] Grape seed extract contains a high concentration of proanthocyanidins, a powerful antioxidant with stronger antioxidant capacity than vitamins C and E; Polyphenol compounds: Grape seeds also contain other polyphenol compounds, such as catechins, epicatechins, etc., which give it a variety of health benefits.

[0021] Cassia seed is a common Chinese medicinal material, which comes from the seeds of the leguminous plant Cassia tora. Cassia seed has the effects of clearing away heat and detoxifying, calming the liver and improving eyesight, and is often used to treat symptoms such as dry eyes, blurred vision, and eye fatigue. It can also help remove liver toxins and improve liver function.

[0022] Mint is a common herb with a refreshing aroma and unique taste. It belongs to the Lamiaceae family, and the most commonly used variety is peppermint. Mint is rich in volatile oils, the main component of which is menthol, as well as other active ingredients such as menthone and lemon. Refreshing: The cooling sensation of mint can stimulate the nervous system, help refresh the mind, and relieve fatigue and stress. Peppermint essential oil is often used in Chinese medicine to help relax the body and mind.

[0023] Compared with the prior art, the advantages and beneficial effects of the present invention are: The present invention extracts bioactive peptides from fish skin, obtains extracts from aloe vera, grape seeds, cassia seeds and mint plants, and is supplemented with hyaluronic acid, witch hazel, arbutin and a vitamin mixture. Under the joint action of the above ingredients, the production of collagen and elastic fibers is stimulated, the elasticity and firmness of the skin are enhanced, and the appearance of fine lines and wrinkles is reduced; the production of melanin is inhibited, pigmentation is lightened, the skin tone is brightened, and dark circles are alleviated; it helps to strengthen the blood vessel wall, promote the flow of lymphatic fluid, reduce eye edema and eye bags; it can promote cell renewal, increase the synthesis of collagen, and improve skin texture. It is applied to the preparation of eye essence, which can achieve a good eye skin care effect. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] The sources of raw materials used in the present invention are as follows: Hyaluronic acid was sourced from Jiangsu Orlis Pharmaceutical Co., Ltd., item number 151; arbutin was sourced from Shanghai Yuanye Biotechnology Co., Ltd., item number S30884; witch hazel was sourced from Shanghai Yuanye Biotechnology Co., Ltd., item number B29162; alkaline protease was sourced from Hefei Tanrun Biotechnology Co., Ltd., item number 320; neutral protease was sourced from Shanghai Yuanye Biotechnology Co., Ltd., item number S10013; papain was sourced from Hefei Hongrui Biotechnology Co., Ltd., item number 589.

[0026] Example 1

[0027] This embodiment provides a method for preparing a composition containing bioactive peptides, comprising the following steps: S1: Boil the pre-treated fish skin at 90° C. for 3 h, filter with 250-350 mesh gauze to obtain a collagen aqueous solution; add 1 g of alkaline protease, 0.8 g of neutral protease and 2 g of papain to 30 g of the collagen aqueous solution, and perform enzymolysis at 60° C. for 3 h; deactivate the enzyme at 80° C. for 40 min, and filter to obtain an enzymolysis solution; use an ultrafiltration membrane package with a molecular weight cutoff of 3 KDa for interception to obtain bioactive peptides; S2: Aloe vera, grape seeds, cassia seeds and mint are mixed and crushed in a mass ratio of 1:2:1.5:3; the mixture is added into 70% ethanol solution with a weight ratio of 10 times, and ultrasonic extraction is performed at a power of 500 W for 45 minutes to obtain a plant mixed extract; S3: Mix 3g of bioactive peptide, 1g of witch hazel, 0.1g of arbutin and 100g of water, heat to 30°C, and stir evenly; S4: Add 6 g of plant extract mixture and 0.5 g of vitamin mixture, heat to 45° C., stir at a rate of 400 r / min for 10 min; and ultrasonically disperse at a power of 600 W for 50 min to obtain a composition containing bioactive peptides.

[0028] Example 2

[0029] This embodiment provides a method for preparing a composition containing bioactive peptides, comprising the following steps: S1: Boil the pre-treated fish skin at 90° C. for 3 h, filter with 250-350 mesh gauze to obtain a collagen aqueous solution; add 3 g alkaline protease, 1.5 g neutral protease and 3 g papain to 50 g collagen aqueous solution, and perform enzymolysis at 60° C. for 3 h; deactivate the enzyme at 80° C. for 40 min, and filter to obtain an enzymolysis solution; use an ultrafiltration membrane package with a molecular weight cutoff of 5 KDa for interception to obtain bioactive peptides; S2: Aloe vera, grape seeds, cassia seeds and mint are mixed and crushed in a mass ratio of 1:3:2.5:4; the mixture is added into 20 times by weight of a 70% ethanol solution, and ultrasonic extraction is performed for 45 minutes to obtain a plant mixed extract; S3: Mix 6 g of bioactive peptide, 3 g of witch hazel, 0.5 g of arbutin and 150 g of water, heat to 40°C, and stir evenly; S4: Add 12 g of the plant extract mixture and 1.5 g of the vitamin mixture, raise the temperature to 55° C., stir at a rate of 600 r / min for 20 min, and ultrasonically disperse at a power of 800 W for 30 min to obtain a composition containing bioactive peptides.

[0030] Example 3

[0031] This embodiment provides a method for preparing a composition containing bioactive peptides, comprising the following steps: S1: Boil the pre-treated fish skin at 90° C. for 3 h, filter with 250-350 mesh gauze to obtain a collagen aqueous solution; add 2 g alkaline protease, 1 g neutral protease and 2.5 g papain to 40 g collagen aqueous solution, and perform enzymolysis at 60° C. for 3 h; deactivate the enzyme at 80° C. for 40 min, and filter to obtain an enzymolysis solution; use an ultrafiltration membrane package with a molecular weight cutoff of 4 KDa for interception to obtain bioactive peptides; S2: Aloe vera, grape seeds, cassia seeds and mint are mixed and crushed in a mass ratio of 1:2.5:2:3.5; the mixture is added to 15 times the weight of a 70% ethanol solution, and ultrasonically extracted for 45 minutes to obtain a plant mixed extract; S3: Mix 5g of bioactive peptide, 2g of witch hazel, 0.3g of arbutin and 130g of water, heat to 35°C, and stir evenly; S4: add 10 g of the plant extract mixture and 1.2 g of the vitamin mixture, raise the temperature to 50° C., stir at a rate of 500 r / min for 16 min; and ultrasonically disperse at a power of 700 W for 45 min to obtain a composition containing bioactive peptides.

[0032] Comparative Example 1 The difference from Example 1 is that only alkaline protease is added in step S1, and the other steps remain unchanged.

[0033] Comparative Example 2 The difference from Example 1 is that only papain is added in step S1, and the other steps remain unchanged.

[0034] Comparative Example 3 The difference from Example 1 is that step S2 is missing, that is, no plant extract is added, and the other steps remain unchanged.

[0035] Comparative Example 4 The difference from Example 1 is that witch hazel is not added in step S3, and the other steps remain unchanged.

[0036] Comparative Example 5 The difference from Example 1 is that arbutin is not added in step S3, and the other steps remain unchanged.

[0037] The composition prepared in each embodiment and comparative example is applied to the preparation of essence, 20g of the composition, 1g of moisturizer pentylene glycol, 0.6g of emulsifier castor oil polyoxyethylene ether-80, 1.5g of thickener guar gum and 0.4g of hyaluronic acid are mixed, stirred evenly at a temperature of 50°C and a speed of 600r / min, and the essence can be obtained after sterilization.

[0038] Performance Test: Anti-wrinkle test: The skin elasticity index R7 value was tested using the skin elasticity meter Cotometer MPA580 from the German CK company. During the test, the negative pressure was kept constant at 450 mbar. The closer the skin elasticity index R7 is to 1, the better the skin elasticity. Tyrosinase inhibition experiment: Tyrosinase with an enzyme activity of 5771 U / mg and 0.5 mmol / L L-DOPA were dissolved in 50 mmol / L NaH2PO4-Na2HPO4 buffer at pH 6.8. Add 0.5 g of freeze-dried mask to 50 ml of L-DOPA solution, keep warm in a 30°C water bath for 10 min, add 1 ml of tyrosinase solution, mix immediately, and measure the absorbance of the reaction solution at 475 nm after 2 min. The results are as follows: Table 1

[0039] From the above performance test results, it can be seen that the compositions containing bioactive peptides prepared in Examples 1-3 have good wrinkle removal, whitening and other properties. In particular, Example 3 has better comprehensive performance. However, since Comparative Examples 1-5 do not adopt the necessary technical solutions, they are significantly worse than the Examples in the corresponding performance tests. The above experimental results further prove the importance of the technical solutions defined in the present invention for its technical effects.

[0040] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composition comprising a bioactive peptide, characterized in that: The composition comprises the following components by weight: 3-6 parts of bioactive peptides, 0.2-0.6 parts of hyaluronic acid, 1-3 parts of witch hazel, 0.1-0.5 parts of arbutin and 100-150 parts of water; A method for preparing a composition containing bioactive peptides comprises the following steps: S1: Extraction of bioactive peptides; S2: preparing a plant extract mixture; S3: Mix 3-6 parts of bioactive peptides, 1-3 parts of witch hazel, 0.1-0.5 parts of arbutin and 100-150 parts of water, heat to 30-40°C, and stir evenly; S4: adding 6-12 parts of the plant extract mixture and 0.5-1.5 parts of the vitamin mixture, heating to 45-55° C., stirring for 10-20 minutes, and then ultrasonically dispersing for 30-50 minutes to obtain a composition containing bioactive peptides; Step S1 is specifically as follows: boiling the pre-treated clean fish skin at 90° C. for 3 hours, filtering with 250-350 mesh gauze to obtain a collagen aqueous solution; adding 1-3 parts of alkaline protease, 0.8-1.5 parts of neutral protease and 2-3 parts of papain to 30-50 parts of the collagen aqueous solution, and performing enzymolysis at a temperature of 60° C. for 3 hours; inactivating the enzyme at a temperature of 80° C. for 40 minutes, and filtering to obtain an enzymolysis solution; and using an ultrafiltration membrane package with a molecular weight cutoff of 3-5 KDa for interception to obtain bioactive peptides.

2. A composition comprising a bioactive peptide according to claim 1, characterized in that: The composition also includes 6-12 parts of plant extract mixture.

3. A composition comprising a bioactive peptide according to claim 2, characterized in that: The composition also includes 0.5-1.5 parts of a vitamin mixture.

4. A composition comprising a bioactive peptide according to claim 3, characterized in that: The bioactive peptides are bioactive peptides extracted from fish.

5. A composition comprising a bioactive peptide according to claim 4, characterized in that: The plant extract mixture is a plant extract mixture of aloe vera, grape seeds, cassia seeds and mint in a mass ratio of 1:2-3:1.5-2.5:3-4.

6. A composition comprising a bioactive peptide according to claim 5, characterized in that: The vitamin mixture comprises vitamin E, vitamin C and vitamin D in a mass ratio of 1:1-2:1-3.

7. A composition comprising a bioactive peptide according to claim 6, characterized in that: Step S2 specifically comprises: mixing and crushing aloe vera, grape seeds, cassia seeds and mint in a mass ratio of 1:2-3:1.5-2.5:3-4; adding into 10-20 times by weight of 70% ethanol solution, and ultrasonically extracting for 45 minutes to obtain a plant mixed extract.

8. Use of a composition comprising a bioactive peptide as claimed in any one of claims 1 to 7 in the preparation of eye care essence.

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