Lifting and tightening composition for skin as well as preparation method and application of lifting and tightening composition

Abstract

The invention relates to a lifting and tightening composition for skin as well as a preparation method and application thereof, and belongs to the technical field of cosmetics, the composition is prepared from the following raw materials: compound fermentation liquor, acetylated sodium hyaluronate, glycosylglycerol and a solvent, the compound fermentation liquor comprises a plant compound ingredient, cellulase, a lactobacillus rhamnosus activating solution, a saccharomyces cerevisiae activating solution and a fruit wine yeast activating solution; the plant compound ingredients comprise Chinese wolfberry fruits, litchi pericarp and grape seeds; the composition also comprises a sophora flower extract, a linden flower extract and Arabic gum. The lycium barbarum, the litchi pericarp and the grape seeds are compounded to serve as raw materials and fermented, the technology of first enzymolysis and then fermentation is adopted, the raw materials supplement one another, and the finished product effectively enables skin to have elasticity; in addition, the finished product prepared by the invention also has the effects of resisting aging, replenishing water and preserving moisture; in other words, multiple aspects in the system cooperate with one another, skin laxity is effectively improved, and the skin is lifted and tightened.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to a skin lifting and firming composition, a preparation method and an application thereof. Background Art

[0002] As people's living standards improve, their interest in skin health and beauty is growing. Skin aging is a natural physiological process, characterized by sagging, increased wrinkling, and decreased elasticity. These phenomena not only affect appearance but can also reduce a person's self-confidence and quality of life. Currently, a wide variety of products are available on the market for skin lifting and firming, primarily including chemically synthesized skincare products, formulations containing bioactive ingredients, and physical therapy.

[0003] However, existing skin-lifting and firming technologies have many limitations. While chemically synthesized skincare products are quick to take effect, they often contain risky chemicals and preservatives, which can lead to skin allergies and dependency with long-term use. While bioactive ingredient preparations are relatively safe, some ingredients struggle to function effectively, potentially impacting the lifting and firming effects. While physical treatments like lasers and radiofrequency can improve skin laxity to a certain extent, they carry risks such as complex procedures, high costs, and the potential for skin damage. Therefore, developing a safe, efficient, and easy-to-prepare skin-lifting and firming composition and its preparation method is crucial to meeting market demand and enhancing skin beauty outcomes. Summary of the Invention

[0004] The purpose of the present invention is to provide a skin lifting and firming composition and its preparation method and application, which solves the problem in the existing cosmetics technology field that the composition added to cosmetics has poor lifting and firming effect.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] A skin lifting and firming composition, comprising the following raw materials:

[0007] Composite fermentation broth, acetylated sodium hyaluronate, glyceryl glucoside and solvent;

[0008] The composite fermentation liquid comprises plant compound ingredients, cellulase, Lactobacillus rhamnosus activation liquid, Saccharomyces cerevisiae activation liquid and fruit wine yeast activation liquid;

[0009] The plant compound ingredients include wolfberry, litchi peel and grape seed.

[0010] As a preferred technical solution of the present invention, the solvent includes but is not limited to deionized water.

[0011] As a preferred technical solution of the present invention, the composite fermentation broth comprises 2-5 parts by weight, acetylated sodium hyaluronate comprises 10-12 parts by weight, glycerol glucoside comprises 5-9 parts by weight, and the solvent comprises 80-85 parts by weight.

[0012] Furthermore, the added amount of the cellulase includes 0.15-0.2% of the total mass of the plant compound, the added amount of the Lactobacillus rhamnosus activation liquid includes 0.2-0.3% of the mass of the plant compound, the added amount of the brewer's yeast activation liquid includes 1.8-2% of the mass of the plant compound, and the added amount of the fruit wine yeast activation liquid includes 2-3% of the mass of the plant compound.

[0013] Furthermore, the cellulase: 10000 U / g, was purchased from Sichuan Huatang Jurui Biotechnology Co., Ltd.

[0014] Furthermore, the Lactobacillus rhamnosus activation liquid, the Saccharomyces cerevisiae activation liquid, and the wine yeast activation liquid are activation liquids obtained by inoculating Lactobacillus rhamnosus, Saccharomyces cerevisiae, and wine yeast into culture media for cultivation. There are no special requirements for the culture media and culture methods used that are different from those known in the art. The key is to ensure that the effective viable bacteria count of the Lactobacillus rhamnosus activation liquid reaches 1-2×10 9 CFU / mL, the effective viable bacteria count of Saccharomyces cerevisiae activation solution reaches 1-1.5x10 6 CFU / mL, the effective viable bacteria count of the wine yeast activation liquid reaches 1.5-2x10 9 The scope of CFU / mL can achieve the purpose of the present invention.

[0015] Preferably, the culture medium selected for the activated Lactobacillus rhamnosus solution and the activated Saccharomyces cerevisiae solution is MRS liquid culture medium; and the culture medium selected for the activated fruit wine yeast solution is YPD liquid culture medium.

[0016] Furthermore, the Lactobacillus rhamnosus was purchased from Hebei Pengyu Biotechnology Co., Ltd.; the brewer's yeast model: XH52808PUI5A, was purchased from Jinan Shenghe Chemical Co., Ltd.; and the fruit wine yeast was purchased from Hebei Runbu Biotechnology Co., Ltd.

[0017] Furthermore, the mass ratio of wolfberry, litchi peel and grape seed is 3-5:1.5-2:8-12;

[0018] Lycium Chineseum is a perennial woody plant of the genus Lycium in the Solanaceae family. It is rich in ingredients such as Lycium barbarum polysaccharides, proanthocyanidins, betaine, B vitamins, amino acids, and quercetin. It promotes the proliferation of fibroblasts, helps skin cells maintain elasticity, is highly effective in anti-oxidation and anti-aging, and has certain moisturizing properties. Lycium barbarum is rarely used as an active ingredient in cosmetics, and fermented Lycium barbarum is even less commonly used as a cosmetic raw material. Therefore, the present invention selects Lycium barbarum and ferments it, which is an innovative addition.

[0019] Litchi (LITCHI CHINENSIS) is an evergreen tree of the genus Litchi in the Sapindaceae family. Litchi peel primarily contains polyphenols, flavonoids, proanthocyanidins, and other ingredients that can effectively promote collagen production and inhibit elastase, helping to maintain skin firmness and reduce sagging. It also enhances the antioxidant capacity of HaCat cells and significantly reduces cell apoptosis caused by UVA irradiation, demonstrating excellent antioxidant activity.

[0020] Grape (VITIS VINIFERA) is a deciduous vine of the Vitaceae family. Grape seeds contain a high level of proanthocyanidins, as well as fat, choline, pantothenic acid, vitamins, etc., which can effectively protect fibroblasts from oxidative damage, stabilize collagen and elastin, and strengthen the skin's structural matrix by supporting elastin fibers, maintaining firmness and elasticity.

[0021] As a preferred technical solution of the present invention, the composition further comprises a Sophora japonica flower extract, a Tilia chinensis flower extract and gum arabic;

[0022] Gum arabic has a complex molecular structure with multiple branches. This unique structure enables it to protect cells from oxidative damage.

[0023] Furthermore, the Sophora japonica flower extract comprises 5-8 parts by weight, the Tilia chinensis flower extract comprises 2-3 parts by weight, and the gum arabic comprises 10-70 parts by weight.

[0024] Furthermore, the Sophora japonica flower extract is obtained by conventional reflux extraction of the flower buds of the deciduous tree Sophora japonica (SOPHORA JAPONICA) of the Leguminosae family using alcohol as a solvent; the Sophora japonica flower extract is rich in flavonoids and triterpenoid saponins, has strong antioxidant and anti-inflammatory effects, can help remove free radicals, and slow down the process of skin aging.

[0025] Furthermore, the mass concentration of the alcohol is 50%, and the material-liquid ratio of the Sophora japonica flower and the solvent is 1g:10mL.

[0026] Furthermore, the linden flower extract is obtained by conventional reflux extraction of the flowers of Tilia vulgaris, a deciduous tree of the genus Tilia of the family Tilia, using alcohol as a solvent; the linden flower extract contains a large amount of flavonoids, triterpenoid saponins, β-sitosterol and other ingredients, which have an inhibitory effect on elastase and caspase, and at the same time, it can activate tissue proteases to a certain extent, that is, the linden flower extract can enhance the metabolism of skin cells and has a good anti-aging effect.

[0027] Furthermore, the mass concentration of the alcohol is 70%, and the material-liquid ratio of the linden flower and the solvent is 1g:10mL.

[0028] A method for preparing a skin-lifting and firming composition comprises the following steps:

[0029] S1. After the plant compound ingredients are mixed evenly, deionized water is added and stirred evenly, the pH is adjusted, and after temperature control and sterilization, cellulase is added and temperature control and enzymatic hydrolysis is performed, and the mixture is placed in a water bath and allowed to stand to obtain an enzymatic hydrolyzate;

[0030] S2, mixing Lactobacillus rhamnosus activated liquid, Saccharomyces cerevisiae activated liquid and fruit wine yeast activated liquid and adding them to the enzymatic hydrolyzate, shaking, controlling the temperature to ferment, obtaining a fermentation material, controlling the temperature to inactivate, filtering and centrifuging to obtain a composite fermentation liquid;

[0031] S3. After uniformly mixing the acetylated sodium hyaluronate, glyceryl glucoside and solvent, add the composite fermentation liquid and stir evenly. Then, add the remaining raw materials and control the temperature and stir evenly to obtain the finished composition.

[0032] As a preferred technical solution of the present invention, the mass ratio of the plant compound to deionized water in step S1 is 1:4-5.

[0033] As a preferred technical solution of the present invention, the pH is adjusted to 4.0-4.5 with citric acid in step S1; the temperature of the temperature-controlled sterilization is 121°C, and the time is 30-35 minutes; the temperature of the temperature-controlled enzymatic hydrolysis is 35-40°C, and the time is 2-2.5 hours; the temperature of the water bath is 70-72°C; and the standing time is 10-12 minutes.

[0034] As a preferred technical solution of the present invention, the temperature of the temperature-controlled fermentation in step S2 is 26-30°C and the time is 48 hours; the temperature of the temperature-controlled inactivation is 121°C and the time is 25-30 minutes.

[0035] As a preferred technical solution of the present invention, the temperature of the temperature control in step S3 is 26-28°C.

[0036] The invention discloses an application of a skin lifting and firming composition in the preparation of a skin care product. The composition is added to the skin care product at a mass percentage of 3-5%.

[0037] Furthermore, the skin care products include but are not limited to moisturizing lotions, essences, lotions and creams.

[0038] Beneficial effects of the present invention:

[0039] (1) The present invention selects wolfberry, litchi peel and grape seeds as raw materials and ferments them, using wolfberry to promote fibroblast proliferation and litchi peel to promote collagen production. Fibroblasts are the main component for producing collagen, and wolfberry can effectively assist litchi peel in increasing collagen production. On this basis, grape seeds are added to protect fibroblasts from oxidative damage to maintain cell health, thereby achieving a continuous supply of collagen. The three in the system complement each other, and the finished product obtained in this way can effectively make the skin have excellent elasticity and avoid the problem of skin sagging.

[0040] (2) The present invention adopts a process of enzymatic hydrolysis followed by fermentation for the raw materials of wolfberry, litchi peel and grape seed. Cellulase is used to first change the permeability of the cell wall to facilitate the leakage of the cell contents. Then, Lactobacillus rhamnosus, Saccharomyces cerevisiae and fruit wine yeast are used as the composite fermentation strains. The strains have a cooperative symbiotic effect, which achieves better growth and adaptation to the environment, and promotes the dissolution of each other's active ingredients. Therefore, the fermentation liquid obtained after the optimization of the enzymatic hydrolysis + fermentation process has a stronger effect on increasing skin collagen and thus increasing skin elasticity.

[0041] (3) The wolfberry and litchi peel used in the present invention not only have the function of increasing collagen after fermentation but also have excellent anti-aging function. Therefore, in order to further strengthen the system function, the present invention also adds Sophora japonica flower extract, Tilia chinensis flower extract and gum arabic, so that the wolfberry can exert high-efficiency antioxidant effect, the litchi peel can reduce cell apoptosis caused by UVA irradiation, the Sophora japonica flower extract can remove free radicals, the Tilia chinensis flower extract can enhance skin cell metabolism, and the gum arabic can protect cells from oxidative damage. The finished composition thus prepared has excellent anti-aging effect.

[0042] (4) The acetylated sodium hyaluronate selected in the present invention can effectively absorb and lock in moisture, keeping the stratum corneum moist. On this basis, a glycoside compound glycerol glucoside, which is formed by connecting glucose and glycerol through a glycosidic bond, is added. Glycerol glucoside reduces water loss and improves skin microcirculation by regulating intracellular osmotic pressure. It can effectively exert a synergistic effect with acetylated sodium hyaluronate, so that the prepared composition has excellent moisturizing effect.

[0043] (5) The finished composition of the present invention can effectively increase collagen, providing a solid support structure for the skin; effectively resist aging, delay skin aging, and reduce collagen loss; effectively replenish and lock in moisture, making the skin more plump and elastic. Supplementing collagen can enhance the skin's water retention capacity, further promoting the hydration effect; the anti-aging ingredients can reduce the damage of free radicals to collagen and moisture. In this way, a positive cycle is formed in the system, and multiple aspects work together to effectively improve the looseness and sagging of the skin, making the skin more lifted, firm, and shiny. DETAILED DESCRIPTION

[0044] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0045] The cellulase used in the Examples, Comparative Examples, and Test Examples of the present invention was 10,000 U / g, purchased from Sichuan Huatang Jurui Biotechnology Co., Ltd.; the Lactobacillus rhamnosus activated solution, Saccharomyces cerevisiae activated solution, and wine yeast activated solution used were obtained by inoculating Lactobacillus rhamnosus and Saccharomyces cerevisiae into MRS liquid medium and culturing them, respectively, and by inoculating wine yeast into YPD liquid medium and culturing them, respectively. There are no special requirements for the culture medium and culture method used that are different from those known in the art;

[0046] The Lactobacillus rhamnosus was purchased from Hebei Pengyu Biotechnology Co., Ltd.; the Saccharomyces cerevisiae model: XH52808PUI5A was purchased from Jinan Shenghe Chemical Co., Ltd.; the fruit wine yeast was purchased from Hebei Runbu Biotechnology Co., Ltd.

[0047] The sophora japonica flower extract used in the Examples, Comparative Examples, and Test Examples of the present invention is obtained by conventional reflux extraction of the flower buds of the deciduous tree Sophora japonica (SOPHORA JAPONICA) of the Leguminosae family with 50% alcohol by mass as a solvent at a material-liquid ratio of 1 g:10 mL; the linden flower extract used is obtained by conventional reflux extraction of the flower of the deciduous tree Tilia genus Tilia of the Tilia family with 70% alcohol by mass as a solvent at a material-liquid ratio of 1 g:10 mL;

[0048] The above is not repeated here.

[0049] Example 1

[0050] A skin lifting and firming composition, comprising the following raw materials in parts by weight:

[0051] 2 parts by weight of composite fermentation broth, 12 parts by weight of acetylated sodium hyaluronate, 5 parts by weight of glycerol glucoside and 80 parts by weight of deionized water;

[0052] The composite fermentation liquid comprises plant compound ingredients, cellulase, Lactobacillus rhamnosus activation liquid, Saccharomyces cerevisiae activation liquid and fruit wine yeast activation liquid;

[0053] The plant compound comprises wolfberry, litchi peel and grape seeds in a mass ratio of 3:1.5:12;

[0054] The cellulase: 10000U / g, the amount of cellulase added is 0.15% of the total mass of the plant compound; the Lactobacillus rhamnosus activation solution: 1.5x10 9 CFU / mL, the amount of Lactobacillus rhamnosus activation solution added is 0.3% of the mass of the plant compound; the Saccharomyces cerevisiae activation solution: 1x10 6 CFU / mL, the amount of brewer's yeast activation solution added is 2% of the mass of the plant compound; the fruit wine yeast activation solution: 1.5x10 9 CFU / mL, the amount of wine yeast activation solution added is 3% of the mass of the plant compound;

[0055] A method for preparing a skin-lifting and firming composition comprises the following steps:

[0056] S1. After the plant compound ingredients are mixed evenly, deionized water is added and stirred evenly. The pH is adjusted to 4.0 with citric acid. After sterilization at 121°C for 30 minutes, cellulase is added and enzymatic hydrolysis is performed at 40°C for 2 hours. The enzymatic hydrolyzate is then placed in a water bath at 72°C for 11 minutes to obtain an enzymatic solution.

[0057] The mass ratio of the plant compound to deionized water is 1:4; the amount of cellulase added is 0.15% of the total mass of the plant compound;

[0058] S2, Lactobacillus rhamnosus activated liquid, Saccharomyces cerevisiae activated liquid and wine yeast activated liquid were mixed evenly and added to the enzymatic hydrolyzate, shaken and fermented at 30°C for 48 hours to obtain a fermentation material, inactivated at 121°C for 25 minutes, filtered and centrifuged to obtain a composite fermentation liquid;

[0059] The amount of the Lactobacillus rhamnosus activation solution added is 0.2% of the mass of the plant compound, the amount of the brewer's yeast activation solution added is 2% of the mass of the plant compound, and the amount of the fruit wine yeast activation solution added is 3% of the mass of the plant compound;

[0060] S3. After evenly mixing acetylated sodium hyaluronate, glyceryl glucoside and deionized water, add the composite fermentation liquid and stir evenly. Then, add the remaining raw materials and control the temperature at 26° C. and stir evenly to obtain the finished composition.

[0061] Example 2

[0062] A skin lifting and firming composition, comprising the following raw materials in parts by weight:

[0063] 5 parts by weight of composite fermentation broth, 10 parts by weight of acetylated sodium hyaluronate, 9 parts by weight of glycerol glucoside and 85 parts by weight of deionized water;

[0064] The composite fermentation liquid comprises plant compound ingredients, cellulase, Lactobacillus rhamnosus activation liquid, Saccharomyces cerevisiae activation liquid and fruit wine yeast activation liquid;

[0065] The plant composite material comprises wolfberry, litchi peel and grape seeds in a mass ratio of 5:1.7:10;

[0066] The cellulase: 10000U / g, the amount of cellulase added is 0.2% of the total mass of the plant compound; the Lactobacillus rhamnosus activation solution: 1x10 9 CFU / mL, the amount of Lactobacillus rhamnosus activation solution added is 0.2% of the mass of the plant compound; the Saccharomyces cerevisiae activation solution: 1x10 6 CFU / mL, the amount of brewer's yeast activation solution added is 1.8% of the mass of the plant compound; the fruit wine yeast activation solution: 2x10 9 CFU / mL, the amount of wine yeast activation solution added is 2% of the mass of the plant compound;

[0067] A method for preparing a skin-lifting and firming composition comprises the following steps:

[0068] S1. After the plant compound ingredients are mixed evenly, deionized water is added and stirred evenly. The pH is adjusted to 4.2 with citric acid. After sterilization at 121°C for 33 minutes, cellulase is added and the temperature is controlled at 35°C for enzymatic hydrolysis for 2 hours. The mixture is then placed in a 70°C water bath and allowed to stand for 10 minutes to obtain an enzymatic hydrolyzate.

[0069] The mass ratio of the plant compound to deionized water is 1:5; the amount of cellulase added is 0.18% of the total mass of the plant compound;

[0070] S2, Lactobacillus rhamnosus activated liquid, Saccharomyces cerevisiae activated liquid and wine yeast activated liquid were mixed evenly and added to the enzymatic hydrolyzate, shaken and fermented at 26 ° C for 48 h to obtain a fermentation material, inactivated at 121 ° C for 27 min, filtered and centrifuged to obtain a composite fermentation liquid;

[0071] The amount of the Lactobacillus rhamnosus activation solution added is 0.25% of the mass of the plant compound, the amount of the brewer's yeast activation solution added is 1.9% of the mass of the plant compound, and the amount of the fruit wine yeast activation solution added is 2% of the mass of the plant compound;

[0072] S3. After evenly mixing acetylated sodium hyaluronate, glyceryl glucoside and deionized water, add the composite fermentation liquid and stir evenly. Then add the remaining raw materials and control the temperature at 28° C. and stir evenly to obtain the finished composition.

[0073] Example 3

[0074] A skin lifting and firming composition, comprising the following raw materials in parts by weight:

[0075] 3.5 parts by weight of composite fermentation broth, 11 parts by weight of acetylated sodium hyaluronate, 7 parts by weight of glycerol glucoside and 82.5 parts by weight of deionized water;

[0076] The composite fermentation liquid comprises plant compound ingredients, cellulase, Lactobacillus rhamnosus activation liquid, Saccharomyces cerevisiae activation liquid and fruit wine yeast activation liquid;

[0077] The plant compound comprises wolfberry, litchi peel and grape seeds in a mass ratio of 4:2:8;

[0078] The cellulase: 10000U / g, the amount of cellulase added is 0.18% of the total mass of the plant compound; the Lactobacillus rhamnosus activation solution: 2x10 9 CFU / mL, the amount of Lactobacillus rhamnosus activation solution added is 0.25% of the mass of the plant compound; the Saccharomyces cerevisiae activation solution: 1.5x10 6 CFU / mL, the amount of brewer's yeast activation solution added is 1.9% of the mass of the plant compound; the fruit wine yeast activation solution: 2x10 9 CFU / mL, the addition amount of wine yeast activation solution is 2.5% of the mass of plant compound;

[0079] A method for preparing a skin-lifting and firming composition comprises the following steps:

[0080] S1. After the plant compound ingredients are mixed evenly, deionized water is added and stirred evenly. The pH is adjusted to 4.5 with citric acid. After sterilization at 121°C for 35 minutes, cellulase is added and enzymatic hydrolysis is carried out at 37°C for 2.5 hours. The enzymatic hydrolyzate is then placed in a water bath at 71°C for 12 minutes to obtain an enzymatic solution.

[0081] The mass ratio of the plant compound to deionized water is 1:4.5; the amount of cellulase added is 0.2% of the total mass of the plant compound;

[0082] S2, Lactobacillus rhamnosus activated liquid, Saccharomyces cerevisiae activated liquid and wine yeast activated liquid were mixed evenly and added to the enzymatic hydrolyzate, shaken and fermented at 28 ° C for 48 h to obtain a fermentation material, inactivated at 121 ° C for 30 min, filtered and centrifuged to obtain a composite fermentation liquid;

[0083] The amount of the Lactobacillus rhamnosus activation solution added is 0.3% of the mass of the plant compound, the amount of the brewer's yeast activation solution added is 1.8% of the mass of the plant compound, and the amount of the fruit wine yeast activation solution added is 2.5% of the mass of the plant compound;

[0084] S3. After evenly mixing acetylated sodium hyaluronate, glyceryl glucoside and deionized water, add the composite fermentation liquid and stir evenly. Then add the remaining raw materials and control the temperature at 27° C. and stir evenly to obtain the finished composition.

[0085] Example 4

[0086] A skin lifting and firming composition, comprising the following raw materials in parts by weight:

[0087] 2 parts by weight of composite fermentation broth, 12 parts by weight of acetylated sodium hyaluronate, 5 parts by weight of glyceryl glucoside, 8 parts by weight of Sophora japonica flower extract, 3 parts by weight of Tilia tree flower extract, 70 parts by weight of gum arabic and 80 parts by weight of deionized water;

[0088] The composite fermentation liquid comprises plant compound ingredients, cellulase, Lactobacillus rhamnosus activation liquid, Saccharomyces cerevisiae activation liquid and fruit wine yeast activation liquid;

[0089] The plant compound comprises wolfberry, litchi peel and grape seeds in a mass ratio of 3:1.5:12;

[0090] The cellulase: 10000U / g, the amount of cellulase added is 0.15% of the total mass of the plant compound; the Lactobacillus rhamnosus activation solution: 1.5x10 9 CFU / mL, the amount of Lactobacillus rhamnosus activation solution added is 0.3% of the mass of the plant compound; the Saccharomyces cerevisiae activation solution: 1x10 6 CFU / mL, the amount of brewer's yeast activation solution added is 2% of the mass of the plant compound; the fruit wine yeast activation solution: 1.5x10 9 CFU / mL, the amount of wine yeast activation solution added is 3% of the mass of the plant compound;

[0091] A method for preparing a skin-lifting and firming composition comprises the following steps:

[0092] S1. After the plant compound ingredients are mixed evenly, deionized water is added and stirred evenly. The pH is adjusted to 4.0 with citric acid. After sterilization at 121°C for 30 minutes, cellulase is added and enzymatic hydrolysis is performed at 40°C for 2 hours. The enzymatic hydrolyzate is then placed in a water bath at 72°C for 11 minutes to obtain an enzymatic solution.

[0093] The mass ratio of the plant compound to deionized water is 1:4; the amount of cellulase added is 0.15% of the total mass of the plant compound;

[0094] S2, Lactobacillus rhamnosus activated liquid, Saccharomyces cerevisiae activated liquid and wine yeast activated liquid were mixed evenly and added to the enzymatic hydrolyzate, shaken and fermented at 30°C for 48 hours to obtain a fermentation material, inactivated at 121°C for 25 minutes, filtered and centrifuged to obtain a composite fermentation liquid;

[0095] The amount of the Lactobacillus rhamnosus activation solution added is 0.2% of the mass of the plant compound, the amount of the brewer's yeast activation solution added is 2% of the mass of the plant compound, and the amount of the fruit wine yeast activation solution added is 3% of the mass of the plant compound;

[0096] S3. After evenly mixing acetylated sodium hyaluronate, glyceryl glucoside and deionized water, add the composite fermentation liquid and stir evenly. Then, add the remaining raw materials and control the temperature at 26° C. and stir evenly to obtain the finished composition.

[0097] Example 5

[0098] A skin lifting and firming composition, comprising the following raw materials in parts by weight:

[0099] 2 parts by weight of composite fermentation broth, 12 parts by weight of acetylated sodium hyaluronate, 5 parts by weight of glyceryl glucoside, 5 parts by weight of Sophora japonica flower extract, 2.5 parts by weight of Tilia tree flower extract, 10 parts by weight of gum arabic and 80 parts by weight of deionized water;

[0100] The composite fermentation liquid comprises plant compound ingredients, cellulase, Lactobacillus rhamnosus activation liquid, Saccharomyces cerevisiae activation liquid and fruit wine yeast activation liquid;

[0101] The plant compound comprises wolfberry, litchi peel and grape seeds in a mass ratio of 3:1.5:12;

[0102] The cellulase: 10000U / g, the amount of cellulase added is 0.15% of the total mass of the plant compound; the Lactobacillus rhamnosus activation solution: 1.5x10 9 CFU / mL, the amount of Lactobacillus rhamnosus activation solution added is 0.3% of the mass of the plant compound; the Saccharomyces cerevisiae activation solution: 1x10 6 CFU / mL, the amount of brewer's yeast activation solution added is 2% of the mass of the plant compound; the fruit wine yeast activation solution: 1.5x10 9 CFU / mL, the amount of wine yeast activation solution added is 3% of the mass of the plant compound;

[0103] A method for preparing a skin-lifting and firming composition comprises the following steps:

[0104] S1. After the plant compound ingredients are mixed evenly, deionized water is added and stirred evenly. The pH is adjusted to 4.0 with citric acid. After sterilization at 121°C for 30 minutes, cellulase is added and enzymatic hydrolysis is performed at 40°C for 2 hours. The enzymatic hydrolyzate is then placed in a water bath at 72°C for 11 minutes to obtain an enzymatic solution.

[0105] The mass ratio of the plant compound to deionized water is 1:4; the amount of cellulase added is 0.15% of the total mass of the plant compound;

[0106] S2, Lactobacillus rhamnosus activated liquid, Saccharomyces cerevisiae activated liquid and wine yeast activated liquid were mixed evenly and added to the enzymatic hydrolyzate, shaken and fermented at 30°C for 48 hours to obtain a fermentation material, inactivated at 121°C for 25 minutes, filtered and centrifuged to obtain a composite fermentation liquid;

[0107] The amount of the Lactobacillus rhamnosus activation solution added is 0.2% of the mass of the plant compound, the amount of the brewer's yeast activation solution added is 2% of the mass of the plant compound, and the amount of the fruit wine yeast activation solution added is 3% of the mass of the plant compound;

[0108] S3. After evenly mixing acetylated sodium hyaluronate, glyceryl glucoside and deionized water, add the composite fermentation liquid and stir evenly. Then, add the remaining raw materials and control the temperature at 26° C. and stir evenly to obtain the finished composition.

[0109] Example 6

[0110] A skin lifting and firming composition, comprising the following raw materials in parts by weight:

[0111] 2 parts by weight of composite fermentation broth, 12 parts by weight of acetylated sodium hyaluronate, 5 parts by weight of glyceryl glucoside, 6.5 parts by weight of Sophora japonica flower extract, 2 parts by weight of Tilia chinensis flower extract, 40 parts by weight of gum arabic, and 80 parts by weight of deionized water;

[0112] The composite fermentation liquid comprises plant compound ingredients, cellulase, Lactobacillus rhamnosus activation liquid, Saccharomyces cerevisiae activation liquid and fruit wine yeast activation liquid;

[0113] The plant compound comprises wolfberry, litchi peel and grape seeds in a mass ratio of 3:1.5:12;

[0114] The cellulase: 10000U / g, the amount of cellulase added is 0.15% of the total mass of the plant compound; the Lactobacillus rhamnosus activation solution: 1.5x10 9 CFU / mL, the amount of Lactobacillus rhamnosus activation solution added is 0.3% of the mass of the plant compound; the Saccharomyces cerevisiae activation solution: 1x10 6 CFU / mL, the amount of brewer's yeast activation solution added is 2% of the mass of the plant compound; the fruit wine yeast activation solution: 1.5x109 CFU / mL, the amount of wine yeast activation solution added is 3% of the mass of the plant compound;

[0115] A method for preparing a skin-lifting and firming composition comprises the following steps:

[0116] S1. After the plant compound ingredients are mixed evenly, deionized water is added and stirred evenly. The pH is adjusted to 4.0 with citric acid. After sterilization at 121°C for 30 minutes, cellulase is added and enzymatic hydrolysis is performed at 40°C for 2 hours. The enzymatic hydrolyzate is then placed in a water bath at 72°C for 11 minutes to obtain an enzymatic solution.

[0117] The mass ratio of the plant compound to deionized water is 1:4; the amount of cellulase added is 0.15% of the total mass of the plant compound;

[0118] S2, Lactobacillus rhamnosus activated liquid, Saccharomyces cerevisiae activated liquid and wine yeast activated liquid were mixed evenly and added to the enzymatic hydrolyzate, shaken and fermented at 30°C for 48 hours to obtain a fermentation material, inactivated at 121°C for 25 minutes, filtered and centrifuged to obtain a composite fermentation liquid;

[0119] The amount of the Lactobacillus rhamnosus activation solution added is 0.2% of the mass of the plant compound, the amount of the brewer's yeast activation solution added is 2% of the mass of the plant compound, and the amount of the fruit wine yeast activation solution added is 3% of the mass of the plant compound;

[0120] S3. After evenly mixing acetylated sodium hyaluronate, glyceryl glucoside and deionized water, add the composite fermentation liquid and stir evenly. Then, add the remaining raw materials and control the temperature at 26° C. and stir evenly to obtain the finished composition.

[0121] Comparative Example 1

[0122] Compared with Example 6, the difference is that no wolfberry is added to the plant composite material in Comparative Example 1, and the plant composite material includes litchi peel and grape seeds in a mass ratio of 3:13.5. The other operating steps and parameters remain unchanged.

[0123] Comparative Example 2

[0124] Compared with Example 6, the difference is that no litchi peel is added to the plant composite material in Comparative Example 2, and the plant composite material includes wolfberry and grape seeds in a mass ratio of 3.75:12.75. The other operating steps and parameters remain unchanged.

[0125] Comparative Example 3

[0126] Compared with Example 6, the difference is that no grape seeds are added to the plant composite material in Comparative Example 3, and the plant composite material includes wolfberry and litchi peel in a mass ratio of 9:7.5. The other operating steps and parameters remain unchanged.

[0127] Comparative Example 4

[0128] Compared with Example 6, the difference is that in step S1 of Comparative Example 4, no cellulase is added for enzymatic hydrolysis, and the other operation steps and parameters remain unchanged.

[0129] Comparative Example 5

[0130] Compared with Example 6, the difference is that in step S2 of Comparative Example 5, no Lactobacillus rhamnosus activation liquid is added, and the addition amount of brewer's yeast activation liquid is 2.15% of the mass of the plant compound; the addition amount of fruit wine yeast activation liquid is 3.15% of the mass of the plant compound, and the other operating steps and parameters remain unchanged.

[0131] Comparative Example 6

[0132] Compared with Example 6, the difference is that in step S2 of Comparative Example 6, no brewer's yeast activation solution is added, and the addition amount of Lactobacillus rhamnosus activation solution is 1.3% of the mass of the plant compound; the addition amount of fruit wine yeast activation solution is 4% of the mass of the plant compound, and the other operating steps and parameters remain unchanged.

[0133] Comparative Example 7

[0134] Compared with Example 6, the difference is that in step S2 of Comparative Example 7, no fruit wine yeast activation liquid is added, the addition amount of Lactobacillus rhamnosus activation liquid is 1.8% of the mass of the plant compound, and the addition amount of brewer's yeast activation liquid is 3.5% of the mass of the plant compound, and the other operating steps and parameters remain unchanged.

[0135] Test Example 1

[0136] Skin elasticity test:

[0137] Volunteers with wrinkles and healthy skin, aged 35-55 years, were recruited and randomly divided into 11 groups of 12 participants each. Each group of volunteers used a cream prepared by adding the composition prepared in Example 1, Examples 4-6, and Comparative Examples 1-7 to the same cream base (the composition was added to the cream at a concentration of 3%). The subjects were instructed to evenly apply 1 mL of the cream to their face, once daily, morning and evening, for four weeks. During the experiment, the subjects were prohibited from applying any other cosmetics to the experimental area.

[0138] The same skin elasticity measuring instrument, Cutometer MPA580, was used to measure the skin elasticity of volunteers before using the product and after four weeks of use. The test site was the application area. The probe was cleaned before each measurement, and the subjects washed the test area with clean water. The probe was repeated three times in the same test area, and the average value was calculated.

[0139] Skin elasticity change rate (%) = (elasticity value at week 4 - elasticity value at week 0) / elasticity value at week 0; specific skin elasticity test results are shown in Table 1.

[0140] Table 1

[0141] Elasticity value at week 0 Elasticity value at week 4 Skin elasticity change rate (%) Example 1 0.674 0.801 17.36 Example 4 0.653 0.778 19.14 Example 5 0.662 0.793 19.79 Example 6 0.669 0.806 20.48 Comparative Example 1 0.671 0.759 13.11 Comparative Example 2 0.659 0.751 13.96 Comparative Example 3 0.692 0.795 14.88 Comparative Example 4 0.684 0.799 16.81 Comparative Example 5 0.665 0.769 15.64 Comparative Example 6 0.678 0.788 16.22 Comparative Example 7 0.654 0.759 16.06

[0142] As can be seen from Table 1, the present invention selects a combination of wolfberry, litchi peel, and grape seed as raw materials and ferments them, which can make the finished composition effectively make the skin have excellent elasticity and avoid the problem of easy skin sagging. At the same time, it can also be shown that the fermentation liquid obtained after the optimization of the enzymatic hydrolysis + fermentation process has a stronger effect on increasing skin collagen and thus increasing skin elasticity.

[0143] Comparative Example 8

[0144] Compared with Example 6, the difference is that no Sophora japonica flower extract is added in Comparative Example 8, and the other operating steps and parameters remain unchanged.

[0145] Comparative Example 9

[0146] Compared with Example 6, the difference is that no linden flower extract is added in Comparative Example 9, and the other operating steps and parameters remain unchanged.

[0147] Comparative Example 10

[0148] Compared with Example 6, the difference is that gum arabic is not added in Comparative Example 10, and the other operating steps and parameters remain unchanged.

[0149] Test Example 2

[0150] DPPH free radical scavenging ability assay:

[0151] Weigh a certain amount of DPPH and prepare a 0.1 mg / mL DPPH solution with anhydrous ethanol. Take 2 mL of the finished composition obtained in Example 1, Examples 4-6, Comparative Examples 1-2, and Comparative Examples 8-10, add 1000 g of sample solution diluted with anhydrous ethanol, add 2 mL of DPPH solution, mix well, react at room temperature for 30 minutes, and then measure the absorbance Ai at 517 nm. Simultaneously measure the absorbance A0 of 2 mL DPPH solution + 2 mL anhydrous ethanol and the absorbance Aj after 2 mL sample solution + 2 mL anhydrous ethanol are mixed. Repeat the measurement twice. The scavenging rate of the sample for DPPH free radicals is calculated using the following formula:

[0152] DPPH free radical scavenging rate (%) = (A0-Aj+Ai) / A0x100%;

[0153] Evaluation criteria: DPPH free radical scavenging ability can reflect antioxidant capacity. The higher the DPPH free radical scavenging rate, the stronger the antioxidant capacity and the better the anti-aging property, and vice versa.

[0154] The results are shown in Table 2.

[0155] Table 2

[0156]

[0157]

[0158] As can be seen from Table 2, the finished composition prepared by the present invention has good DPPH free radical scavenging ability and excellent anti-aging effect.

[0159] Comparative Example 11

[0160] Compared with Example 6, the difference is that in Comparative Example 11, no acetylated sodium hyaluronate is added and acetylated sodium hyaluronate is replaced with glycerol glucoside in equal parts by weight, and the other operating steps and parameters remain unchanged.

[0161] Comparative Example 12

[0162] Compared with Example 6, the difference is that in Comparative Example 12, glycerol glucoside is not added and is replaced by acetylated sodium hyaluronate in equal parts by weight of glycerol glucoside, and the other operating steps and parameters remain unchanged.

[0163] Test Example 3

[0164] Hydrating and moisturizing test: Volunteers aged 35-55 years old, half male and half female, were recruited and randomly divided into 6 groups, with 12 in each group. Each group of volunteers used a cream made by adding the composition prepared in Example 1, Examples 4-6 and Comparative Examples 11-12 to the same cream base (the amount of the composition added to the cream was 3%). The method of use was to apply 1 mL of cream evenly on the face of the subject, once in the morning and evening each day, for 4 consecutive weeks. During the experiment, the subjects could not apply any other cosmetics on the experimental area.

[0165] Before using the product and up to the fourth week of use, the skin moisture content of the applied area was measured using the same skin moisture tester. The probe was cleaned before each measurement, and the subjects washed the test area with clean water. The probe was repeated three times in the same test area, and the average value was taken. The results are shown in Table 3.

[0166] Table 3

[0167]

[0168]

[0169] As can be seen from Table 3, the finished composition prepared by the present invention has excellent moisturizing and hydrating effects.

[0170] Throughout the specification, references to terms such as "one embodiment," "example," or "specific example" indicate that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or example are included in at least one embodiment or example of the present invention. In this specification, schematic representations of these terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0171] The above content is merely an example and explanation of the concept of the present invention. Those skilled in the art may make various modifications or additions to the described specific embodiments or replace them in a similar manner. As long as they do not deviate from the concept of the invention or exceed the scope defined by the claims, they should all fall within the scope of protection of the present invention.

Claims

1. A skin lifting and firming composition, characterized in that: The composition comprises the following raw materials: Composite fermentation broth, sodium acetylated hyaluronate, glyceryl glucoside and solvent; The composite fermentation liquid comprises plant compound ingredients, cellulase, Lactobacillus rhamnosus activation liquid, Saccharomyces cerevisiae activation liquid and fruit wine yeast activation liquid; The plant compound ingredients include wolfberry, litchi peel and grape seed.

2. The skin lifting and firming composition according to claim 1, characterized in that: The composite fermentation broth comprises 2-5 parts by weight, acetylated sodium hyaluronate comprises 10-12 parts by weight, glycerol glucoside comprises 5-9 parts by weight, and the solvent comprises 80-85 parts by weight.

3. The skin lifting and firming composition according to claim 1, characterized in that The added amount of the cellulase includes 0.15-0.2% of the total mass of the plant compound, the added amount of the rhamnosus lactobacillus activation liquid includes 0.2-0.3% of the mass of the plant compound, the added amount of the brewer's yeast activation liquid includes 1.8-2% of the mass of the plant compound, and the added amount of the wine yeast activation liquid includes 2-3% of the mass of the plant compound.

4. The skin lifting and firming composition according to claim 1, wherein The cellulase: 10000U / g, the effective viable count of the Lactobacillus rhamnosus activation solution: 1-2x10 9 CFU / mL, effective viable count of Saccharomyces cerevisiae activation solution: 1-1.5x10 6 CFU / mL, effective viable count of wine yeast activation solution: 1.5-2x10 9 CFU / mL.

5. The skin lifting and firming composition according to claim 1, characterized in that: The mass ratio of wolfberry, litchi peel and grape seed is 3-5:1.5-2:8-12.

6. The skin lifting and firming composition according to claim 1, characterized in that The composition also includes Sophora japonica flower extract, Tilia oleracea flower extract and gum arabic.

7. The skin lifting and firming composition according to claim 6, characterized in that: The sophora japonica flower extract comprises 5-8 parts by weight, the linden flower extract comprises 2-3 parts by weight, and the gum arabic comprises 10-70 parts by weight.

8. A method for preparing the skin lifting and firming composition according to any one of claims 6 to 7, characterized in that: The steps include: S1. After the plant compound ingredients are mixed evenly, deionized water is added and stirred evenly, the pH is adjusted, and after temperature control and sterilization, cellulase is added and temperature control and enzymatic hydrolysis is performed, and the mixture is placed in a water bath and allowed to stand to obtain an enzymatic hydrolyzate; S2, mixing Lactobacillus rhamnosus activated liquid, Saccharomyces cerevisiae activated liquid and fruit wine yeast activated liquid and adding them to the enzymatic hydrolyzate, shaking, controlling the temperature to ferment, obtaining a fermentation material, controlling the temperature to inactivate, filtering and centrifuging to obtain a composite fermentation liquid; S3. After uniformly mixing the acetylated sodium hyaluronate, glyceryl glucoside and solvent, add the composite fermentation liquid and stir evenly. Then, add the remaining raw materials and control the temperature and stir evenly to obtain the finished composition.

9. The method for preparing the skin lifting and firming composition according to claim 8, characterized in that: The mass ratio of the plant compound and deionized water in step S1 is 1:4-5.

10. Use of a skin lifting and firming composition in the preparation of a skin care product, wherein the composition is added to the skin care product at a mass percentage of 3-5%.

Images