Application of angelica sinensis in promoting hair growth

Abstract

The invention provides an application of angelica sinensis in promoting hair growth. Specifically, the invention provides application of a traditional Chinese medicine angelica sinensis or an extract thereof in preparation of a composition or a preparation capable of improving hair damage caused by external glucocorticoid. The invention also provides a composition containing the angelica sinensis or the extract thereof, application of the composition, and a method for improving hair damage by using the angelica sinensis or the extract thereof. Experiments show that the angelica sinensis or the extract thereof can effectively improve the hair damage degree, the hair growth cycle and the in-vivo hormone level, so that the newborn hair coverage area and the newborn hair length are increased, the hair rest period is shortened, the hair growth period is advanced, and the hair follicle growth state is improved. Wide application prospects are realized in the fields of treating hair injury, alopecia and the like caused by hormones.

Description

Technical Field

[0001] The present invention relates to the field of medicine, and in particular to the use of Xigui to promote hair growth. Background Art

[0002] As an appendage of the skin, hair has many functions such as regulating body temperature, participating in the remodeling of the skin microenvironment, and aesthetic modification, which have an impact on the individual's personal and social image. In addition to genetic factors, irregular diet and daily routines and increasing life and work pressures have made hair loss an increasingly hot topic in society, and the phenomenon of hair loss has gradually become younger and more professional. Although abnormal hair loss does not affect the body's life functions, the changes in appearance caused by it will cause individuals to experience a certain degree of psychological pressure and mental burden.

[0003] Clinically, hair loss is divided into androgenic alopecia, alopecia areata, senile alopecia, etc., among which androgenic alopecia is the most common. Currently, only minoxidil and finasteride are the only two drugs approved by the US Food and Drug Administration for hair regeneration and prevention of hair loss, but their side effects limit the number of people who can use them.

[0004] As a treasure of the Chinese nation, Chinese herbal medicine has a splendid cultural background, contains profound health and wellness concepts, and is the result of thousands of years of medical practice. Screening and discovering Chinese herbal medicines with high safety and the potential to promote hair regeneration and prevent hair loss based on the Chinese herbal medicine library is of great significance to the development and advancement of this field.

[0005] Therefore, there is an urgent need in the art to develop a new class of drugs that promote hair growth and prevent hair loss. Summary of the invention

[0006] The main purpose of the present invention is to provide a Chinese herbal medicine capable of promoting hair growth, namely, Xigui. Specifically, the present invention relates to the use of the Chinese herbal medicine Xigui in the preparation of hair growth promoting drugs, health products or cosmetics.

[0007] Specifically, the present invention proves through experiments that the water extract of Xigui can effectively restore the hair growth state of mice affected by fluocinolone, shorten the hair resting phase and induce the early growth phase of hair, accelerate the growth of new hair and increase the coverage area of ​​new hair in mice, improve the abnormal morphology and growth inhibition of hair follicles in mice with hair damage induced by fluocinolone, increase the number of terminal hair in mice, reduce the ratio of vellus hair to terminal hair, and significantly increase the maximum diameter of hair follicles. Therefore, Xigui has broad prospects in the field of promoting hair growth and preventing hair loss.

[0008] The first aspect of the present invention provides a use of an active ingredient or a preparation containing the active ingredient, characterized in that it is used to prepare a preparation or composition for promoting hair growth; wherein the active ingredient is quinquefolius or quinquefolius extract.

[0009] In another preferred example, the Xigui extract is selected from the following group: a water extract of Xigui, an organic solvent extract of Xigui, or a combination thereof.

[0010] In another preferred embodiment, the organic solvent is selected from the following group: methanol, ethanol, petroleum ether, ethyl acetate, n-butanol, or a combination thereof.

[0011] In another preferred embodiment, the composition or preparation is used for one or more purposes selected from the following group:

[0012] (i) Improve hair damage;

[0013] (ii) Improve hair growth cycle;

[0014] (iii) Improve hormone levels in the body.

[0015] In another preferred embodiment, the composition or preparation is used for one or more purposes selected from the following group:

[0016] (i) Increase the area covered by new hair;

[0017] (ii) Increase the length of new hair;

[0018] (iii) shortening the hair resting phase;

[0019] (iv) Advance the hair growth phase.

[0020] In another preferred embodiment, the improvement of hair damage includes increasing the coverage area of ​​new hair and / or increasing the length of new hair.

[0021] In another preferred embodiment, the coverage area of ​​the newly grown hair increases by 25-40%, preferably by 28-38%, and more preferably by 30-36%.

[0022] In another preferred embodiment, the length of the newly grown hair increases by 4.0 to 4.5 mm, preferably by 4.1 to 4.3 mm.

[0023] In another preferred embodiment, the improving the hair growth cycle includes shortening the hair resting phase and / or advancing the hair growth phase.

[0024] In another preferred embodiment, the shortening of the hair resting phase and / or advancing the hair growth phase refers to advancing the skin graying time and the hair appearance time.

[0025] In another preferred embodiment, the improving hormone levels in the body includes increasing hormone levels, and the hormones are selected from the following group: androgens, glucocorticoids, thyroid hormones, pituitary hormones, adrenocortical hormones, or a combination thereof.

[0026] In another preferred embodiment, the composition or preparation is used to increase the maximum diameter of hair follicles.

[0027] In another preferred embodiment, the composition or preparation is used to increase the maximum diameter of hair follicles by 35 to 45 μm, preferably by 45 to 55 μm.

[0028] In another preferred embodiment, the hair damage is damage induced by external hormones.

[0029] In another preferred embodiment, the hair damage is damage induced by topical glucocorticoid fluocinolone.

[0030] In another preferred embodiment, the preparation or composition is in the form of injection or oral administration.

[0031] The second aspect of the present invention provides a composition, which is a cosmetic composition, a food composition, a health product composition or a pharmaceutical composition, and contains:

[0032] (A) as the first active ingredient, radix quinquefolii or a radix quinquefolii extract;

[0033] (B) a second active ingredient having a hair growth promoting effect; and

[0034] (C) a pharmaceutically, food or cosmetically acceptable carrier or excipient.

[0035] In another preferred embodiment, the Xigui extract is selected from the following group: a water extract of Xigui, an organic solvent extract of Xigui, or a combination thereof.

[0036] In another preferred embodiment, the organic solvent is selected from the following group: methanol, ethanol, petroleum ether, ethyl acetate, n-butanol, or a combination thereof.

[0037] In another preferred embodiment, the second active ingredient is selected from the following group: vitamin A, vitamin C, vitamin E, collagen peptide, hyaluronic acid, apigenin, chlorogenic acid, ferulic acid, kojic acid, niacinamide, arbutin, or a combination thereof.

[0038] The third aspect of the present invention provides a use of the composition according to the second aspect of the present invention for preparing medicines, foods, health products or cosmetics for improving hair damage.

[0039] In another preferred embodiment, the medicine, food, health product or cosmetic is used for one or more purposes selected from the following group:

[0040] (i) Improve hair damage;

[0041] (ii) Improve hair growth cycle;

[0042] (iii) Improve hormone levels in the body.

[0043] In another preferred embodiment, the medicine, food, health product or cosmetic is used for one or more purposes selected from the following group:

[0044] (i) Increase the area covered by new hair;

[0045] (ii) Increase the length of new hair;

[0046] (iii) shortening the hair resting phase;

[0047] (iv) Advance the hair growth phase.

[0048] In another preferred embodiment, the improvement of hair damage includes increasing the coverage area of ​​new hair and / or increasing the length of new hair.

[0049] In another preferred embodiment, the coverage area of ​​the newly grown hair increases by 25-40%, preferably by 28-38%, and more preferably by 30-36%.

[0050] In another preferred embodiment, the length of the newly grown hair increases by 4.0 to 4.5 mm, preferably by 4.1 to 4.3 mm.

[0051] In another preferred embodiment, the improving the hair growth cycle includes shortening the hair resting phase and / or advancing the hair growth phase.

[0052] In another preferred embodiment, the shortening of the hair resting phase and / or advancing the hair growth phase refers to advancing the skin graying time and the hair appearance time.

[0053] In another preferred embodiment, the improving hormone levels in the body includes increasing hormone levels, and the hormones are selected from the following group: androgens, glucocorticoids, thyroid hormones, pituitary hormones, adrenocortical hormones, or a combination thereof.

[0054] In another preferred embodiment, the composition or preparation is used to increase the maximum diameter of hair follicles.

[0055] In another preferred embodiment, the composition or preparation is used to increase the maximum diameter of hair follicles by 35 to 45 μm, preferably by 45 to 55 μm.

[0056] In another preferred embodiment, the hair damage is damage induced by external hormones.

[0057] In another preferred embodiment, the hair damage is damage induced by topical glucocorticoid fluocinolone.

[0058] In another preferred embodiment, the drug, food, health product or cosmetic is in the form of injection or oral administration.

[0059] The fourth aspect of the present invention provides a method for improving hair damage and / or promoting hair growth, comprising the steps of administering to a subject the Chinese medicine Xigui, Xigui extract, a preparation containing Xigui extract, or a composition as described in the second invention of the present invention.

[0060] In another preferred example, the Xigui extract is selected from the following group: a water extract of Xigui, an organic solvent extract of Xigui, or a combination thereof.

[0061] In another preferred embodiment, the organic solvent is selected from the following group: methanol, ethanol, petroleum ether, ethyl acetate, n-butanol, or a combination thereof.

[0062] In another preferred embodiment, the subject is a mammal, preferably a rodent or a primate, more preferably a mouse, a rat or a human.

[0063] It should be understood that within the scope of the present invention, the above-mentioned technical features of the present invention and the technical features specifically described below (such as embodiments) can be combined with each other to form a new or preferred technical solution. Due to space limitations, they will not be described one by one here. BRIEF DESCRIPTION OF THE DRAWINGS

[0064] Figure 1 The images show the effect of water extract of Xigui on hair growth in mice with fluocinolone-induced hair damage.

[0065] Figure 2 The results showed that the water extract of Xigui increased the new hair coverage area and hair length of mice with hair damage induced by fluocinolone, and advanced the graying time of the mice's skin and the appearance time of new hair. *** indicates P<0.001. # indicates P < 0.05, ## indicates P < 0.01, ### Indicates P<0.001.

[0066] Figure 3 It was shown that the aqueous extract of Xigui restored the abnormal hair follicle morphology and growth inhibition in mice with fluocinolone-induced hair damage.

[0067] Figure 4 The results showed that the water extract of Xigui increased the number of terminal hair in mice with hair damage induced by fluocinolone, reduced the ratio of vellus hair to terminal hair, and significantly increased the maximum diameter of hair follicles. * indicates P<0.05, ** indicates P<0.01, *** indicates P<0.001, # indicates P < 0.05, ## indicates P < 0.01, ### Indicates P<0.001.

[0068] Figure 5The images show the effects of Xigui water extract, Xigui polysaccharide and alcohol precipitation supernatant on hair growth in mice with fluocinolone-induced hair damage.

[0069] Figure 6 The results showed that the water extract, polysaccharide and alcohol precipitation supernatant of Xigui could increase the new hair coverage area and hair length of mice with hair damage induced by fluocinolone, and advance the time of skin graying and the appearance of new hair in mice. *** indicates P<0.001, # indicates P<0.05, and ## indicates P<0.01. DETAILED DESCRIPTION

[0070] After extensive and in-depth research, the inventor unexpectedly discovered that the traditional Chinese medicine Xigui can promote hair growth, especially can effectively improve hair damage caused by topical glucocorticoids. On this basis, the inventor completed the present invention.

[0071] the term

[0072] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

[0073] As used herein, when used in reference to a specific recited numerical value, the term "about" means that the value may vary by no more than 1% from the recited value. For example, as used herein, the expression "about 100" includes all values ​​between 99 and 101 (e.g., 99.1, 99.2, 99.3, 99.4, etc.).

[0074] As used herein, the term "comprising" or "including (comprising)" may be open, semi-closed and closed. In other words, the term also includes "consisting essentially of" or "consisting of".

[0075] Active ingredient of the present invention and preparation method thereof

[0076] Return to the West

[0077] Chinese Angelica thibetica is the root of the plant Vicatia thibetica de Boiss. of the Apiaceae family, also known as Angelica thibetica, wild Angelica thibetica, etc., and is mainly distributed in places such as Yunnan, Sichuan, and Tibet. Chinese Angelica thibetica is warm in nature, sweet in taste, and slightly bitter, and has the effects of regulating menstruation, invigorating qi, and tonifying blood. Artificial cultivation is planted in some areas of Dali, Yunnan, and Chinese Angelica thibetica can be stewed with pig's feet, chicken, etc. as edible tonic dishes, and it is highly safe. At present, there is no report that it improves the skin atrophy induced by glucocorticoid.

[0078] As used herein, the terms "the extract of the present invention", "the active ingredient of the present invention", and "the composition or preparation of the present invention" are used interchangeably and all refer to the elm tree and its extract extracted from the elm tree plant and having the function of promoting hair growth.

[0079] The extract of Xigui includes water extract of Xigui and organic solvent extract of Xigui, wherein the organic solvent is selected from the group consisting of methanol, ethanol, petroleum ether, ethyl acetate, n-butanol, or a combination thereof.

[0080] In the present invention, the active ingredient having the function of promoting hair growth is extracted from quince as a raw material.

[0081] In addition, although the effective parts with the function of promoting hair growth can come from the whole plant of the Umbelliferae genus, which mainly includes roots, stems and other parts, they can also be extracted from the above-ground parts of the Umbelliferae genus, such as branches and leaves.

[0082] As used herein, the term "extract" includes water-soluble and / or fat-soluble extracts. The term also includes alcohol extracts, ester extracts or water extracts.

[0083] According to analysis, the chemical components contained in the effective part of the present invention at least include substances selected from the following group: sugars, flavonoids, desphenolic acid, etc.

[0084] The method for preparing the extract of the plant of the genus Apiaceae of the present invention is not particularly limited. A water-soluble and / or fat-soluble extract can be obtained by conventional methods using the plant of the genus Apiaceae as a raw material.

[0085] In another preferred embodiment, the preparation of the effective fraction is carried out by solvent extraction, extraction, and / or chromatography.

[0086] In the present invention, there is no particular limitation on the solvent used in the solvent extraction method, and representative examples include (but are not limited to): water, ethanol, methanol, acetone, ethyl acetate, or a mixed solvent of one or more solvents. The extraction times can be one or more.

[0087] In the present invention, there is no particular limitation on the solvent used in the solvent extraction method. Representative examples include (but are not limited to): n-butanol, ethyl acetate, dichloromethane, chloroform, cyclohexane, petroleum ether, or a mixture of one or more solvents. The extraction times may be one or more times;

[0088] In the present invention, there is no particular limitation on column chromatography, and representative examples include (but are not limited to): activated carbon, silica gel, reverse phase silica gel, macroporous resin, dextran gel or a combination of several thereof.

[0089] In another preferred embodiment, the radix quinquefolii of the genus Apiaceae is used as a raw material, and is extracted 1-5 times with water (or ethanol aqueous solution) 2-10 times the weight of the medicinal material, and the water is recovered to obtain a water extract. The water extract is dissolved in 0.5-6 times the weight of ethanol (preferably 70-99% ethanol), the ethanol supernatant is set aside, the precipitate is dissolved in water, and then extracted with petroleum ether or ethyl acetate to obtain an ester extract. The extract is subjected to LC-MS analysis, and its main components are sugars, flavonoids, phenolic acid, etc. The precipitate is adjusted in density with purified water, and radix quinquefolii polysaccharides can be obtained after high-temperature drying.

[0090] Pharmaceutical compositions and methods of administration

[0091] The present invention also provides a composition, preparation or product containing the active ingredient of the present invention, which can be used to promote hair growth. Representative compositions, preparations or products include hair growth promoting drugs, health products, and cosmetics.

[0092] The pharmaceutical composition of the present invention comprises:

[0093] (a) an aqueous extract of Radix Angelicae Pubescentis as the first active ingredient; and / or

[0094] (b) other ingredients having hair growth promoting effects selected from the group consisting of Angelica sinensis, Polygonum multiflorum, Platycladus orientalis, or a combination thereof; and

[0095] (c) a pharmaceutically acceptable carrier or excipient.

[0096] As used herein, the term "effective amount" or "effective dose" refers to an amount that can produce a function or activity (ie, promote hair growth function) on humans and / or animals and can be accepted by humans and / or animals.

[0097] As used herein, the term "pharmaceutically acceptable" ingredients are suitable for use in humans and / or mammals without excessive adverse side effects (such as toxicity, irritation and allergic reactions), i.e., substances with a reasonable benefit / risk ratio. The term "pharmaceutically acceptable carrier" refers to a carrier for administration of a therapeutic agent, including various excipients and diluents.

[0098] The pharmaceutical composition of the present invention contains a safe and effective amount of the active ingredient of the present invention and a pharmaceutically acceptable carrier. Such carriers include (but are not limited to): saline, buffer, glucose, water, glycerol, ethanol, and combinations thereof. Usually, the pharmaceutical preparation should match the mode of administration. The dosage form of the pharmaceutical composition of the present invention is injection, oral preparation (tablet, capsule, oral liquid), transdermal agent, sustained release agent. For example, it is prepared by conventional methods using physiological saline or an aqueous solution containing glucose and other adjuvants. The pharmaceutical composition is preferably manufactured under sterile conditions.

[0099] In another preferred embodiment, the composition or preparation is in the form of a solid preparation, a liquid preparation or a semisolid preparation.

[0100] In another preferred embodiment, the composition or preparation is in the form of an oral preparation, an external preparation or an injection preparation.

[0101] In another preferred embodiment, the composition or preparation is in the form of an oral preparation.

[0102] Typically, the composition or preparation is in the form of a tablet, injection, infusion, ointment, gel, solution, microsphere or film.

[0103] The effective amount of the active ingredient of the present invention may vary with the mode of administration and the severity of the disease to be treated. The selection of the preferred effective amount can be determined by a person of ordinary skill in the art based on various factors (e.g., through clinical trials). The factors include, but are not limited to: pharmacokinetic parameters of the active ingredient such as bioavailability, metabolism, half-life, etc.; the severity of the disease to be treated by the patient, the patient's body weight, the patient's immune status, the route of administration, etc. Generally, when the active ingredient of the present invention is administered at a dose of about 0.00001g-6g / kg animal body weight (preferably 0.0001g-2.4g / kg animal body weight) per day, satisfactory results can be obtained. For example, depending on the urgency of the treatment situation, several divided doses may be administered per day, or the dose may be reduced proportionally.

[0104] Typically, when the pharmaceutical composition of the present invention is administered orally, the average daily dose for a 60 kg body weight subject (human) is usually 1-30 g, preferably 2-20 g, more preferably 5-12 g. The daily dose can be taken once, twice or multiple times.

[0105] The pharmaceutically acceptable carriers of the present invention include (but are not limited to): water, saline, liposomes, lipids, peptides, cellulose, nanogels, or combinations thereof. The choice of carrier should match the mode of administration, which are well known to those of ordinary skill in the art.

[0106] Compared with the prior art, the main advantages of the present invention include:

[0107] 1. The present invention unexpectedly discovered for the first time that the Chinese medicine Xigui has the effect of promoting hair growth;

[0108] 2. The Xigui extract of the present invention (especially the water extract, polysaccharide, and alcohol precipitation) has a significant ability to promote hair regeneration;

[0109] 3. The Xigui and Xigui extracts of the present invention have the effect of improving hair damage and improving the hair growth cycle;

[0110] 4. The active ingredients of the present invention are derived from Chinese herbal medicines and are safe and effective.

[0111] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are intended to illustrate the present invention only and are not intended to limit the scope of the present invention. The experimental methods in the following examples where specific conditions are not specified are usually performed under conventional conditions, such as those described in Sambrook et al., Molecular Cloning: A Laboratory Manual (New York: Cold Spring Harbor Laboratory Press, 1989), or under conditions recommended by the manufacturer. Unless otherwise indicated, percentages and parts are weight percentages and weight parts.

[0112] Example 1 Preparation of water extracts of Radix Angelicae Pubescentis and Platycladus orientalis

[0113] Weigh 10 kg of dried roots of Xigui, crush them with a grinder, decoct them with distilled water at 80°C for 3 times, with a solid-liquid ratio of 1:10, and 90 minutes each time. Combine the extracts, concentrate under reduced pressure to obtain a concentrate, and freeze-dry in vacuum to obtain Xigui water extract powder. Weigh 90, 180, and 360 mg of Xigui water extract powder, add 1.5 ml of deionized water, respectively, and mix thoroughly until completely dissolved to obtain 0.6, 1.2, and 2.4 g / kg Xigui water extract solutions.

[0114] Weigh 20 kg of Platycladus orientalis powder, boil it twice with distilled water at 100°C with a solid-liquid ratio of 1:15, each time for 1 hour, combine the extracts, concentrate under reduced pressure to obtain an extract, and vacuum freeze-dry to obtain the Platycladus orientalis water extract powder. Weigh 90 mg of Platycladus orientalis water extract powder, add 1.5 ml of deionized water respectively, mix thoroughly until completely dissolved, and obtain 0.6 g / kg Platycladus orientalis water extract solution.

[0115] Example 2 Glucocorticoid-induced hair damage in mice and administration of extracts

[0116] Experimental methods: 6-week-old male SPF-grade C57BL / 6J mice (purchased from Jicui Yaokang) weighing (22±2g) were randomly divided into 6 groups after one week of feeding, with 6 mice in each group: blank control, fluocinolone, water extract of Xigui (0.6g / kg), water extract of Xigui (1.2g / kg), water extract of Xigui (2.4g / kg), and water extract of Platycladus orientalis (0.6g / kg). The back area of ​​mice in all groups was depilated one day before modeling and administration, and the depilated area was about 2cm×3cm. Except for the blank control group, 200μL of 5μg / mL fluocinolone solution (dissolved in 40% isopropanol) was applied externally on the depilated area every 72h to simulate the inhibition of hair growth induced by external adverse factors. The blank control group mice were applied with an equal volume of 40% isopropanol every 72h, for a total of 9 times.

[0117] At the same time, the mice in the blank control group and the fluocinolone acetonide group were gavaged with 200 μl of deionized water daily, the mice in the quinquefolius water extract group were gavaged with 200 μl of quinquefolius water extract of different concentrations daily, and the mice in the arborvitae water extract group were gavaged with 200 μl of arborvitae water extract daily. The administration lasted for 28 days.

[0118] The administration and modeling were carried out simultaneously, and the mice in each group were anesthetized and photographed every two days to observe the growth of hair on the back of the mice. On the 29th day of the experiment, the mice were killed by cervical dislocation, and a part of the back skin tissue was taken for paraffin embedding and sectioning, and another part of the back skin was quickly frozen and stored at -80℃, all of which were used for subsequent experiments.

[0119] Example 3 Verification of the effect of water extract of Chinese angelica on shortening the resting phase of hair, inducing the early growth phase of hair, accelerating the growth of new hair and increasing the coverage area of ​​new hair in mice

[0120] The mouse model was constructed by the method described in Example 2 and different concentrations of water extract of Xigui were administered. Each group of mice was anesthetized and photographed every two days, and the first skin graying time, the first new hair appearance time and the length of new hair of each mouse were recorded. The images after shooting were used Image J software to calculate the ratio of the new hair coverage area to the total area of ​​the back shaved area. Except for the fluocinolone group, all data were compared with the fluocinolone group to calculate significant differences (One-way ANOVA / T-test). Graph Pad was used for mapping, and the error bars indicated the standard error of the mean (SEM).

[0121] The experimental results are as follows Figure 1 As shown, on the 0th day of administration, the back skin of mice in each group was pink and white; on the 10th day of administration, except for the fluocinolone group with no significant change, the back skin of mice in other groups began to turn gray; on the 14th day of administration, except for the fluocinolone group with no significant change, new villi appeared on the back of mice in other groups; on the 18th day of administration, except for the fluocinolone group with no significant change, new hair grew significantly on the back of mice in other groups, and the coverage area expanded; on the 28th day of administration, the mice in the blank control group were fully covered with hair, the mice in the fluocinolone group still had no signs of hair, and the mice in the different concentrations of the angelica water extract groups and the Platycladus orientalis water extract groups grew longer new hair, and the coverage area further expanded.

[0122] The experimental statistical results are as follows Figure 2As shown, compared with the blank control group, the time of first skin graying and first hair appearance of mice in the fluocinolone acetonide group was significantly delayed, *** indicates P<0.001, while the time of first skin graying and first hair appearance of mice in the 0.6 g / kg, 1.2 g / kg and 2.4 g / kg groups were significantly shortened compared with the fluocinolone acetonide group, # indicates P<0.05, ## indicates P<0.01, and ### indicates P<0.001, and by shortening the hair resting phase and inducing the hair growth phase in advance, the hair cycle is accelerated, which is beneficial to the regrowth of hair.

[0123] At the same time, the hair length of mice in each group on the 28th day was counted. Compared with the blank control group, the hair length of mice in the fluocinolone group was significantly reduced, *** indicates P < 0.001, while each drug-treated group significantly increased the length of new hair of mice compared with the fluocinolone group, ## indicates P < 0.01. At the same time, the new hair coverage area of ​​mice in each time period was counted. Compared with the blank control group, the new hair coverage area of ​​mice in the fluocinolone group was significantly reduced, while each drug-treated group significantly increased the new hair coverage area of ​​mice compared with the fluocinolone group. In addition, from 14 to 24 days of drug administration, the proportion of new hair coverage area of ​​mice treated with the water extract of Xigui (0.6g / kg) was greater than that of the water extract of Platycladus orientalis (0.6g / kg). The above results all indicate that the water extract of Xigui has a better potential to promote hair growth.

[0124] Example 3: The water extract of Xigui restored the abnormal hair follicle morphology and growth inhibition in mice with hair damage induced by fluocinolone

[0125] A mouse model was constructed according to the method described in Example 2 and different concentrations of water extract of Xigui were administered. The mice were killed and paraffin sections of the skin of mice in different groups were obtained and HE staining was performed. The staining results were photographed using a Nikon Ti2 inverted fluorescence microscope.

[0126] The experimental results are as follows Figure 3 As shown, compared with the blank control group, most hair follicles of the mice in the fluocinolone acetonide group had abnormal morphology and were still in the resting phase, while most hair follicles of the mice in the angelica sinensis water extract (0.6 g / kg), angelica sinensis water extract (1.2 g / kg) and angelica sinensis water extract (2.4 g / kg) groups had normal morphology and entered the growth phase, black arrows indicate early growth phase hair follicles, red arrows indicate resting phase hair follicles, triangles indicate miniaturized hair follicles, scale bar = 100 μm.

[0127] Example 4. The aqueous extract of Xigui increases the number of terminal hairs in mice with hair damage induced by fluocinolone, reduces the ratio of vellus hair to terminal hair, and significantly increases the maximum diameter of hair follicles

[0128] According to the method described in Example 2, a mouse model was constructed and different concentrations of Xigui water extract were given. After killing mice and obtaining paraffin sections of different groups of mouse skin, HE staining was performed. The staining results were taken using a Nikon Ti2 inverted fluorescence microscope. The images after shooting were used to count the number of mouse vellus hair and terminal hair in the visual field using Image J software, and the total number of hair follicles and the vellus hair / terminal hair ratio were calculated, and the maximum diameter of each hair follicle in the visual field was counted. Except for the fluocinolone group, all data were compared with the fluocinolone group to calculate significant differences (One-way ANOVA / T-test). Graph Pad was used for mapping, and the error bar marked the standard error of the mean (SEM).

[0129] The experimental results are as follows Figure 4 As shown, the number of vellus hair and terminal hair of mice in the visual field was counted, and the total number of hair follicles and the ratio of vellus hair to terminal hair were calculated. Compared with the blank control group, the number of vellus hair, terminal hair and total number of hair follicles in the fluocinolone acetonide group were significantly reduced, * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001, while the number of terminal hair of mice in the Xigui water extract (0.6 g / kg) group, Xigui water extract (1.2 g / kg) group and Xigui water extract (2.4 g / kg) group were significantly increased, # indicates P < 0.05, ## indicates P < 0.01. In addition, compared with the blank control group, the vellus hair / terminal hair ratio of mice in the fluocinolone acetonide group was significantly increased, *** indicates P < 0.001, while the vellus hair / terminal hair ratio of mice in the elixir water extract (0.6 g / kg) group, elixir water extract (1.2 g / kg) group and elixir water extract (2.4 g / kg) group were all significantly reduced, ### indicates P < 0.001, indicating that elixir water extract can significantly promote the growth and development of mouse hair and induce the hair to enter the growth period.

[0130] At the same time, compared with the blank control group, the maximum diameter of the hair follicles in the fluocinolone acetonide group was significantly reduced, *** indicates P < 0.001, while the maximum diameter of the hair follicles in the 0.6 g / kg, 1.2 g / kg and 2.4 g / kg groups were significantly increased, indicating that the 1.2 g / kg water extract can effectively improve the growth state of hair follicles, # indicates P < 0.01, scale bar = 100 μm. In addition, compared with the 0.6 g / kg water extract, the 0.6 g / kg water extract of the 1.2 g / kg mice had a lower ratio of vellus hair to terminal hair and a larger maximum diameter of hair follicles, indicating that the 1.2 g / kg water extract has a better effect in promoting hair growth.

[0131] Example 5 Preparation of Xigui polysaccharide and alcohol precipitation supernatant samples

[0132] According to Example 1, 45 and 90 mg of Xigui water extract powder were weighed, 1.5 ml of deionized water was added respectively, and the mixture was thoroughly mixed until completely dissolved to obtain 0.3 and 0.6 g / kg Xigui water extract solutions.

[0133] Weigh 10kg of dried roots of Xigui, crush them in a grinder, decoct them with distilled water at 80℃ for 3 times, with a solid-liquid ratio of 1:10, 90 minutes each time, combine the extracts, decompress and concentrate to a density of 1.2 to obtain a concentrated solution, add 30% ethanol for alcohol precipitation, with a solid-liquid ratio of 1:4, centrifuge and take the supernatant after standing for 12 hours, decompress and concentrate again to obtain a concentrated solution, then add 90% ethanol for secondary alcohol precipitation, with a solid-liquid ratio of 1:10, centrifuge and take the precipitate after standing for 12 hours, add purified water to adjust the density to 1.04, and dry at a spray drying temperature of 190℃ to obtain Xigui polysaccharide powder. Take the supernatant after the second alcohol precipitation and centrifugation, decompress and concentrate to obtain a concentrated solution, and freeze-dry in vacuum to obtain an alcohol precipitation supernatant powder. Weigh 45 and 90mg of Xigui polysaccharide / alcohol precipitation supernatant powder, add 1.5ml of deionized water respectively, mix thoroughly until completely dissolved, and obtain 0.3 and 0.6g / kg Xigui polysaccharide / alcohol precipitation supernatant solutions.

[0134] Example 6 Verifies that the water extract of Xigui, Xigui polysaccharide, and alcohol precipitation supernatant can shorten the hair resting phase, induce the hair growth phase to advance, accelerate the growth of new hair, and increase the new hair coverage area of ​​mice

[0135] The mouse model was constructed according to the method described in Example 2 and different concentrations of water extract of Xigui, Xigui polysaccharide and alcohol precipitation supernatant were administered. Each group of mice was anesthetized and photographed every two days, and the first skin graying time, the first new hair appearance time and the length of new hair of each mouse were recorded. The images after shooting were used Image J software to calculate the ratio of the new hair coverage area to the total area of ​​the back shaved area. Except for the fluocinolone group, all data were compared with the fluocinolone group to calculate significant differences (One-way ANOVA / T-test). Graph Pad was used for mapping, and the error bars indicated the standard error of the mean (SEM).

[0136] The experimental results are as follows Figure 5As shown, on the 0th day of administration, the back skin of mice in each group was pink and white; on the 10th day of administration, except for the blank control group, there was no obvious change in the back skin of mice in other groups; on the 14th day of administration, except for the fluocinolone acetonide group and the Platycladus orientalis water extract (0.6 g / kg) group, the backs of mice in other groups began to turn gray; on the 18th day of administration, except for the fluocinolone acetonide group, new hair appeared on the backs of mice in other groups; on the 22nd day of administration, except for the fluocinolone acetonide group, new hair grew significantly on the backs of mice in other groups, and the coverage area expanded; on the 28th day of administration, the mice in the blank control group were fully covered with hair, the mice in the fluocinolone acetonide group grew hair in some areas, and the mice in the remaining groups grew longer new hair, and the coverage area further expanded.

[0137] The experimental statistical results are as follows Figure 6 As shown, compared with the blank control group, the time of first skin graying and first hair appearance of mice in the fluocinolone acetonide group was significantly delayed, *** indicates P < 0.001, while the Xigui water extract (0.3g / kg, 0.6g / kg) group, Xigui polysaccharide (0.3g / kg, 0.6g / kg) group and alcohol precipitation supernatant (0.3g / kg, 0.6g / kg) group significantly shortened the time of first skin graying of mice compared with the fluocinolone acetonide group, # indicates P < 0.05, ## indicates P < 0.01. In addition, the Xigui polysaccharide (0.3g / kg, 0.6g / kg) group and the alcohol precipitation supernatant (0.3g / kg, 0.6g / kg) group significantly shortened the time when the mouse hair first appeared, # indicates P<0.05. Both showed a stronger ability to shorten the hair resting phase and induce the early hair growth phase than Xigui water extract, and the effects of Xigui water extract, Xigui polysaccharide and alcohol precipitation supernatant were better than Platycladus orientalis water extract.

[0138] At the same time, the hair length of mice in each group on the 28th day was counted. Compared with the blank control group, the hair length of mice in the fluocinolone acetonide group was significantly reduced, *** indicates P < 0.001, while the radix elatior water extract (0.6 g / kg) group, radix elatior polysaccharide (0.6 g / kg) group and alcohol precipitation supernatant (0.6 g / kg) group significantly increased the length of new hair of mice, # indicates P < 0.05. The effects of the three were better than those of the radix elatior water extract (0.6 g / kg) group. At the same time, the new hair coverage area of ​​mice in each time period was counted. Compared with the blank control group, the new hair coverage area of ​​mice in the fluocinolone acetonide group was significantly reduced, while the new hair coverage area of ​​mice in each drug-treated group was significantly increased compared with the fluocinolone acetonide group. In addition, from 14 to 28 days after drug administration, the proportions of new hair coverage area of ​​mice treated with quinquefolius water extract (0.6 g / kg), quinquefolius polysaccharides (0.6 g / kg) and alcohol precipitation supernatant (0.6 g / kg) were all greater than those treated with Platycladus orientalis water extract (0.6 g / kg). The above results indicate that quinquefolius water extract, quinquefolius polysaccharides and alcohol precipitation supernatant have good potential to promote hair growth, and at the same concentration, the three are better than Platycladus orientalis water extract in promoting hair growth.

[0139] All documents mentioned in the present invention are cited as references in this application, just as each document is cited as reference individually. In addition, it should be understood that after reading the above teachings of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the claims attached to this application.

Claims

1. Use of an active ingredient or a preparation containing the active ingredient, characterized in that: Used for preparing a preparation or composition for promoting hair growth; wherein the active ingredient is quinquefolius or quinquefolius extract.

2. The use according to claim 1, characterized in that The Xigui extract is selected from the following group: a water extract of Xigui, an organic solvent extract of Xigui, or a combination thereof.

3. The use according to claim 1, characterized in that The composition or preparation is used for one or more purposes selected from the following group: (i) Improve hair damage; (ii) Improve hair growth cycle; (iii) Improve hormone levels in the body.

4. The use according to claim 1, characterized in that The hair damage is damage induced by external hormones.

5. The use according to claim 1, characterized in that The preparation or composition is in the form of injection or oral administration.

6. A composition, which is a cosmetic composition, a food composition, a health product composition or a pharmaceutical composition, and which contains: (A) as the first active ingredient, radix quinquefolii or a radix quinquefolii extract; (B) a second active ingredient having a hair growth promoting effect; and (C) a pharmaceutically, food or cosmetically acceptable carrier or excipient.

7. The composition according to claim 6, characterized in that The Xigui extract is selected from the following group: a water extract of Xigui, an organic solvent extract of Xigui, or a combination thereof.

8. The composition according to claim 6, characterized in that The second active ingredient is selected from the group consisting of vitamin A, vitamin C, vitamin E, collagen peptides, hyaluronic acid, apigenin, chlorogenic acid, ferulic acid, kojic acid, niacinamide, arbutin, or a combination thereof.

9. Use of the composition according to claim 6, characterized in that: Used for preparing medicines, foods, health products or cosmetics for improving hair damage.

10. A method for improving hair damage and / or promoting hair growth, characterized in that: The method comprises the steps of administering the Chinese medicine Xigui, Xigui extract, a preparation containing Xigui extract or the composition as claimed in claim 6 to the subject.

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