Earthworm reinforced purification type constructed wetland capable of relieving antibiotic stress and construction method of earthworm reinforced purification type constructed wetland

Abstract

The invention discloses a tremomblus verrucosus enhanced purification type constructed wetland for relieving antibiotic stress and a construction method thereof, and relates to the technical field of water pollution treatment and ecological restoration. The constructed wetland comprises a substrate layer, a wetland plant layer and a benthonic animal layer, wherein benthonic animals are earthworm. According to the construction method, sewage containing antibiotics is purified through the constructed wetland by adopting a sequencing batch type upward water flowing and overflowing water discharging mode. According to the constructed wetland disclosed by the invention, the nitrogen removal efficiency of the constructed wetland under the stress of antibiotics is remarkably improved, and the removal rate of the antibiotics in sewage is also remarkably improved.

Description

Technical Field

[0001] The present application relates to the technical field of water pollution control and ecological restoration, and in particular to an earthworm-enhanced purification artificial wetland for alleviating antibiotic stress and a construction method thereof. Background Art

[0002] Constructed wetlands are an effective way to purify sewage by utilizing the interaction between aquatic plants, animals, substrates, and microorganisms. However, their purification effect is affected by the internal and external environment of the wetland. For example, low soil porosity limits oxygen diffusion in the substrate, insufficient carbon sources, high nitrogen concentrations, and other pollutants can reduce their pollutant removal rate. Therefore, paying attention to stress factors and developing mitigation strategies are crucial to maintaining the function and ecological integrity of constructed wetlands.

[0003] Due to their wide application and low treatment efficiency, antibiotics have become a new type of pollutant in aquatic ecosystems and sewage. Studies have shown that artificial wetlands can reduce the content of antibiotics in sewage through absorption and degradation. However, antibiotics can also reduce the purification performance of artificial wetlands by disrupting plant metabolism and affecting the structure and function of carbon and nitrogen cycle microbial communities, thereby affecting the sustainability of their ecological functions. At present, research focuses on technologies related to the purification of antibiotics by artificial wetlands, but there is less attention paid to the reverse effects of antibiotics on the functions of artificial wetlands and how to mitigate such effects. This limits the improvement and enhancement of the purification function of artificial wetlands. Summary of the invention

[0004] The present invention aims to solve at least one of the technical problems existing in the prior art or related art.

[0005] To this end, the first aspect of the present invention provides a tremuloid-enhanced purification artificial wetland for alleviating antibiotic stress, comprising:

[0006] A matrix layer; the matrix layer includes a soil layer, a first gravel layer and a second gravel layer in order from top to bottom, wherein the gravel particle size of the first gravel layer is smaller than the gravel particle size of the second gravel layer;

[0007] A wetland plant layer; the wetland plant layer is planted on the soil layer;

[0008] benthic animal layer; the benthic animal layer is added into the soil layer, and the benthic animals in the benthic animal layer are earthworms;

[0009] A drainage outlet; the drainage outlet is located above the soil layer;

[0010] Water inlet; the water inlet is located at the bottom of the second gravel layer, and the water inlet is connected to a sewage storage device containing antibiotics.

[0011] Furthermore, the artificial wetland also includes: an anti-clogging net; the anti-clogging net is set at two locations, one of which is set at the front end of the drain outlet, and the other is set between the soil layer and the first gravel layer.

[0012] Anti-clogging net; the anti-clogging net is arranged at the front end of the drain outlet.

[0013] Furthermore, the wetland plant is Iris calamus, and the transplanting density of Iris calamus is 50 to 60 plants / m 2 .

[0014] Furthermore, the density of adding earthworms is 1.25×10 4 ~1.5×10 4 pcs / m 2 .

[0015] Furthermore, the height of the soil layer is 8 to 12 cm;

[0016] The gravel particle size of the first gravel layer is 0.9 to 1.6 cm, and the height of the first gravel layer is 5 to 6 cm;

[0017] The gravel particle size of the second gravel layer is 1.6-3.0 cm, and the height of the second gravel layer is 28-32 cm.

[0018] The second aspect of the present invention provides a method for constructing a tremuloid-enhanced purification artificial wetland for alleviating antibiotic stress, the method for constructing the artificial wetland comprising:

[0019] Filling the matrix layer, including filling the second gravel layer, the first gravel layer and the soil layer in sequence from bottom to top;

[0020] Planting wetland plants, planting wetland plants on the soil layer;

[0021] Add benthic animals and earthworms to the soil layer to alleviate antibiotic stress;

[0022] The inlet and outlet water are configured by adopting the method of sequential batch upward inlet and overflow outlet, and the inlet water is sewage containing antibiotics.

[0023] Furthermore, the wetland plant is Iris calamus, and the Iris calamus transplanting method is: select Iris calamus with a height of 18 to 30 cm and similar leaf numbers and leaf sizes, pre-cultivate for 6 to 8 days, and transplant after its growth is stable, with a transplanting density of 50 to 60 plants / m 2 .

[0024] Furthermore, the method of adding earthworms to the soil layer includes: adding earthworms in a batch manner, adding earthworms at 0 days, 30±2 days, 60±2 days and 90±2 days respectively, and the density of earthworm addition is 1.25×104 ~1.5×10 4 pcs / m 2 .

[0025] Furthermore, after the upward water inflow, the hydraulic retention time (HRT) is 2 to 4 days.

[0026] Furthermore, when the concentrations of oxytetracycline (OTC), sulfamethoxazole (SM2) and ciprofloxacin (CIP) in the sewage containing antibiotics were all 100 μg / L, the above-mentioned artificial wetland had a significantly lower NH4 + -N average removal rate increased by more than 25%, NO x - The average removal rate of -N increased by more than 50%, the average removal rate of COD increased by more than 32%, the average removal rate of TN increased by more than 28%, the average removal rate of oxytetracycline increased by more than 13%, the average removal rate of ciprofloxacin increased by more than 9%, and the average removal rate of sulfamethazine increased by more than 6%.

[0027] Compared with the prior art, the present invention has at least the following beneficial effects:

[0028] The present invention introduces benthic animal Earthworms into artificial wetlands and adopts sequencing batch upward water inflow and overflow outflow to purify sewage containing antibiotics through the artificial wetlands constructed by the present invention, thereby significantly improving the nitrogen removal efficiency of the artificial wetlands under antibiotic stress and significantly improving the removal rate of antibiotics in sewage. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Various other advantages and benefits will become apparent to those of ordinary skill in the art by reading the following detailed description of the preferred embodiment.The accompanying drawings are only for the purpose of illustrating the preferred embodiments and are not to be considered as limiting the present application.

[0030] Figure 1 Example 1 of the present invention provides a trembling earthworm enhanced purification type artificial wetland system for alleviating antibiotic stress.

[0031] The reference numerals are as follows: 1. wetland plant layer; 2. soil layer; 3. benthic animal layer; 4. anti-clogging net; 5. first gravel layer; 6. second gravel layer; 7. drain outlet; 8. water inlet; 9. sewage storage device containing antibiotics. DETAILED DESCRIPTION

[0032] In order to better understand the above-mentioned technical scheme, the technical scheme of the embodiments of the present application is described in detail below through the accompanying drawings and specific embodiments. It should be understood that the embodiments of the present application and the specific features in the embodiments are detailed descriptions of the technical scheme of the embodiments of the present application, rather than limitations on the technical scheme of the present application. In the absence of conflict, the embodiments of the present application and the technical features in the embodiments may be combined with each other.

[0033] A first aspect of an embodiment of the present invention provides a tremuloid-enhanced purification artificial wetland for alleviating antibiotic stress, comprising:

[0034] Matrix layer; the matrix layer includes a soil layer 2, a first gravel layer 5 and a second gravel layer 6 from top to bottom, wherein the gravel particle size of the first gravel layer 5 is smaller than the gravel particle size of the second gravel layer 6;

[0035] Wetland plant layer 1; the wetland plant layer 1 is planted on the soil layer 2;

[0036] benthic animal layer 3; the benthic animal layer 3 is added into the soil layer 2, and the benthic animals in the benthic animal layer 3 are earthworms;

[0037] Drainage outlet 7; the drainage outlet 7 is located above the soil layer 2;

[0038] Water inlet 8; the water inlet 8 is located at the bottom of the second gravel layer 6, and the water inlet 8 is connected to the sewage storage device 9 containing antibiotics.

[0039] The artificial wetland provided by the embodiment of the present invention, through the introduction of earthworms, on the one hand, based on its biological disturbance, feeding behavior and interaction with aquatic plants, respectively utilizes the matrix microenvironment to create adsorption, bioaccumulation and root absorption mechanisms, thereby weakening the stress of antibiotics on the artificial wetland; on the other hand, forms a matrix-animal-plant-microorganism multi-link composite purification system, extends the wetland purification chain, and enhances the purification effect of pollutants in the artificial wetland.

[0040] In some embodiments, the artificial wetland further includes: an anti-clogging net 4 ; the anti-clogging net 4 is provided at two locations, one of which is provided at the front end of the drain outlet 7 , and the other is provided between the soil layer 2 and the first gravel layer 5 .

[0041] It can be understood that one anti-clogging net 4 is arranged at the top drain outlet 7 of the artificial wetland system, at the front end of the drain outlet 7, to prevent the drain outlet 7 from being blocked, thereby ensuring smooth drainage; another anti-clogging net 4 is arranged between the soil layer 2 and the first gravel layer 5, which, on the one hand, blocks fine soil particles from entering the coarser gravel layer, thereby preventing mixing between different materials. On the other hand, when water flows through the soil layer and the gravel layer, the anti-clogging net 4 can reduce the scouring effect of water flow on the soil layer 4. At the same time, it can also prevent soil particles from clogging the pores in the gravel layer, thereby maintaining the permeability and drainage function of the gravel layer. Optionally, the anti-clogging net 4 is an anti-clogging nylon net.

[0042] In some embodiments, the wetland plant 1 is Iris calamus, and the transplanting density of Iris calamus is 50 to 60 plants / m 2 .

[0043] If the density is too small, it is not conducive to sewage purification; if the density is too large, its growth condition is poor and the purification capacity is low.

[0044] In some embodiments, the density of the addition of earthworms is 1.25×10 4 ~1.5×10 4 pcs / m 2 .

[0045] In some embodiments, the height of the soil layer 2 is 8 to 12 cm, and preferably the height of the soil layer 2 is 10 cm; the gravel particle size of the first gravel layer 5 is 0.9 to 1.6 cm, the height of the first gravel layer 5 is 5 to 6 cm, and the height of the first gravel layer 5 is preferably 5.5 cm; the gravel particle size of the second gravel layer 6 is 1.6 to 3.0 cm, the height of the second gravel layer 6 is 28 to 32 cm, and the height of the second gravel layer 6 is preferably 30 cm.

[0046] Specifically, although small-sized gravel can provide a larger specific surface area, which is conducive to the attachment of microorganisms and improves the efficiency of pollutant removal, its gap is small, which is easy to cause clogging problems and affect the life of the system. In order to balance the interception efficiency and anti-clogging requirements, a layered laying method can be adopted: the upper layer (the first gravel layer 5) uses small-sized gravel to improve the pollutant interception effect, and the lower layer (the second gravel layer 6) uses large-sized gravel to reduce the risk of clogging, thereby ensuring the long-term stable operation of the system.

[0047] A second aspect of the embodiments of the present invention provides a method for constructing a tremuloid-enhanced purification artificial wetland for alleviating antibiotic stress, comprising:

[0048] Filling the matrix layer, including filling the second gravel layer 6, the first gravel layer 5 and the soil layer 2 in sequence from bottom to top;

[0049] Planting wetland plants: Plant wetland plants on soil layer 2;

[0050] Add benthic animals and add earthworms to soil layer 2 to alleviate antibiotic stress;

[0051] The inlet and outlet water are configured by adopting the method of sequential batch upward inlet and overflow outlet, and the inlet water is sewage containing antibiotics.

[0052] The method for constructing an earthworm-enhanced purification artificial wetland for alleviating antibiotic stress provided in an embodiment of the present invention selects the benthic animal earthworm as the driving factor, and combines the sequencing batch upward water inflow and overflow water outflow to develop a method for constructing an artificial wetland sewage treatment system that integrates matrix-animal-plant and microorganism multi-purification functional modules and a purification chain extension type to cope with antibiotic stress, which effectively alleviates the adverse effects of antibiotics on the purification efficiency of wetlands. Earthworms, benthic animals, are introduced into the artificial wetland, and the sequencing batch upward water inflow and overflow water outflow are used to purify the sewage containing antibiotics through the artificial wetland constructed by the present invention, which significantly improves the efficiency of artificial wetlands in removing nitrogen from sewage under antibiotic stress (significantly improves the removal of NH4 + -N, NO x - -N, COD and TN purification effects), and the removal rate of antibiotics in sewage was also significantly improved.

[0053] It is understandable that earthworms play multiple roles in the system: reducing antibiotic content, improving the matrix microenvironment, activating plant purification functions, optimizing the structure and function of microbial communities, and enhancing the connectivity and synergy of the matrix-animal-plant-microbe modules. Its bioturbation effect further increases the porosity and dissolved oxygen content of the matrix layer, effectively preventing blockage and ensuring the system's efficient purification performance and long-term stable operation.

[0054] Furthermore, the upward water inlet can effectively prevent the clogging of the matrix and replenish the dissolved oxygen in the system; the overflow effluent prolongs the residence time of the sewage in the matrix layer, enhances the contact between the pollutants and the matrix, forms an excellent static adsorption environment, and promotes the adsorption and degradation of antibiotics. The sequencing batch inlet and outlet water prevents clogging while creating an alternating aerobic and anaerobic environment, further improving the efficiency of pollutant removal. In addition, this design is easy to manage and maintain, and significantly optimizes the overall treatment effect of the system. The sequencing batch upward water inlet and overflow effluent method can optionally ensure a hydraulic retention time of 2 to 4 days, and the preferred hydraulic retention time is 3 days.

[0055] In some embodiments, the wetland plant is Iris calamus, and the Iris calamus transplanting method is: select Iris calamus with a height of 18 to 30 cm and similar leaf numbers and leaf sizes, pre-cultivate for 6 to 8 days, and transplant after its growth is stable, with a transplanting density of 50 to 60 plants / m 2 .

[0056] Specifically, Iris phellodendron, with its strong adaptability and pollution resistance, and relying on its developed root system, can significantly promote microbial activity and improve wastewater treatment efficiency. Its root system not only helps to transport oxygen to the root zone and support the activity of aerobic microorganisms, but also directly absorbs some antibiotics, providing multiple supports for wastewater purification. Optionally, the Iris phellodendron transplanting process: select Iris phellodendrons with a height of 18 to 30 cm and similar leaf numbers and leaf sizes for pre-cultivation for 7 days, and after their growth stabilizes, transplant them to the artificial wetland system with a transplanting density of 50 to 60 plants / m 2 And ensure sufficient light and water conditions. If the density of yellow iris is too small, it is not conducive to sewage purification; if the density is too large, its growth condition is poor and the purification capacity is low.

[0057] In some embodiments, earthworms are added to the soil layer 2, the method comprising: adding earthworms in a batch manner, using earthworms to alleviate antibiotic stress, adding earthworms at 0 days, 30±2 days, 60±2 days and 90±2 days, respectively, and the density of earthworm addition is 1.25×10 4 ~1.5×10 4 pcs / m 2 .

[0058] Specifically, antibiotics weaken the purification capacity and ecological balance of the artificial wetland system by inhibiting the biological disturbance and metabolic activities of earthworms. Adding earthworms in a batch manner can help earthworms gradually adapt to the ecological environment containing antibiotics, significantly improve the degradation efficiency of antibiotics, and enhance the overall purification capacity of the system. Preferably, earthworms are added at 0, 30, 60 and 90 days, respectively.

[0059] It is understandable that too high a density of earthworms will intensify competition and affect their survival rate and activity efficiency; too low a density will lead to insufficient treatment efficiency. After comprehensive consideration, the density of earthworm addition was controlled at 1.25×10 4 ~1.5×10 4 pcs / m 2 This density can provide enough biomass to efficiently treat pollutants, promote the decomposition of organic matter and nutrient cycling, while avoiding the negative impact caused by too high or too low density, thereby maintaining ecological balance and ensuring stable and efficient operation of the system.

[0060] In some embodiments, after the upstream water inflow, the hydraulic retention time is 2 to 4 days.

[0061] Specifically, the retention time after water inflow is beneficial to the artificial wetland system in removing pollutants in sewage, such as nitrogen and antibiotics, but too long a retention time will reduce the working efficiency of the artificial wetland system; optionally, the hydraulic retention time is 3 days. An inlet pipe is provided at the bottom of the second gravel layer 6 to meet the upward water inflow requirements.

[0062] In summary, the earthworm enhanced purification artificial wetland for alleviating antibiotic stress and the construction method thereof provided by the embodiments of the present invention have the following technical effects:

[0063] 1) The present invention introduces the common benthic animal earthworms and adopts a batch addition method to make them more adaptable to artificial wetland systems containing antibiotics. The first batch of earthworms initially degrade antibiotics through bioturbation and metabolism in saturated vertical flow artificial wetlands, improve the wetland environment, create better conditions for the survival of subsequent earthworms and increase their survival rate. As the number of earthworms gradually increases, their ecological regulation ability is enhanced, the efficiency of antibiotic degradation is further improved, and the purification function of the wetland system is significantly enhanced.

[0064] 2) The sequencing batch inflow and overflow water discharge method involving earthworms significantly improved the functional performance of the constructed wetland system. The upward inflow effectively prevents the matrix from being blocked and replenishes the system with dissolved oxygen; the overflow water discharge prolongs the residence time of sewage in the matrix layer, enhances the contact between pollutants and the matrix, forms an excellent static adsorption environment, and promotes the adsorption and degradation of antibiotics. While preventing blockage, the sequencing batch inflow and outflow creates an alternating aerobic and anaerobic environment, further improving the efficiency of pollutant removal. In addition, earthworms increase the porosity and dissolved oxygen content of the matrix layer through bioturbation, avoiding blockage and maintaining stable operation of the system.

[0065] 3) The present invention constructs a multi-purification module and purification chain extension type artificial wetland under antibiotic stress by introducing earthworms. Earthworms not only reduce the antibiotic content in the system, but also improve the matrix microenvironment, activate the plant purification function, optimize the structure and function of the microbial community, and strengthen the connectivity and synergy between the matrix-animal-plant-microorganism multifunctional modules, thereby effectively resisting the negative effects of antibiotics and ensuring the efficient purification performance of the artificial wetland.

[0066] 4) The present invention requires little investment, is easy to operate, has significant ecological and environmental benefits, and is easy to apply and promote.

[0067] Example 1 A tremuloid-enhanced purification artificial wetland for alleviating antibiotic stress and a method for constructing the same

[0068] like Figure 1 As shown, the artificial wetland system is designed as follows: the main body is an organic glass body with a length of 0.2m, a width of 0.2m, and a height of 0.5m. A water inlet pipe 8 is provided at the bottom of one side of the system, a sewage storage device 9 is provided at the source of the water inlet pipe, a drain outlet 7 is provided at the top of the system, and an anti-clogging net 4 is provided at the top outlet of the system. The anti-clogging net 4 can be an anti-clogging nylon net. The system includes a wetland plant layer 1, a benthic animal layer 3, and a matrix layer. The matrix layer is composed of a soil layer 2 (10cm high), a small-size gravel layer 5 (particle size is 0.9-1.6cm, height is 5.5cm), and a large-size gravel layer 6 (particle size is 1.6-3.0cm, height is 30cm).

[0069] The planting density of Iris pumila is 55 plants / m 2 , the transplanting steps are as follows:

[0070] 1) Select Iris calamus with a height of 18 to 30 cm and the same number and size of leaves;

[0071] 2) Pre-cultivation for 7 days until the growth is stable;

[0072] 3) Transplanting Iris pumila into artificial wetland system;

[0073] 4) Ensure adequate light and water conditions.

[0074] Earthworms were added in a batch method at 0, 30, 60 and 90 days. The amount of earthworms added was 1.25×10 4 ~1.5×10 4 pcs / m 2 .

[0075] The method of sequential batch upflow and overflow outflow is adopted. Preparation method of sewage containing antibiotics: artificial water distribution is used to simulate actual sewage, with glucose, ammonium chloride and potassium nitrate as raw materials, and the concentration ratio is as follows: NH4 + -N:10.24±0.28mg / L,NO x - -N: 9.71 ± 0.29 mg / L, TN: 20.28 ± 0.5 mg / L, COD: 64.50 ± 2.05 mg / L. In addition, three common typical antibiotics (oxytetracycline, sulfadimethoxine and ciprofloxacin) were added to the domestic sewage, each with a concentration of 100 μg / L and a hydraulic retention time of 3 days.

[0076] Comparative Example 1

[0077] The difference between Comparative Example 1 and Example 1 is that the artificial wetland system of Comparative Example 1 does not have a benthic animal layer 3 .

[0078] Comparative Example 2

[0079] The difference between Comparative Example 2 and Example 1 is that the sewage entering the artificial wetland system of Comparative Example 2 does not contain antibiotics.

[0080] Comparative Example 3

[0081] The difference between Comparative Example 3 and Example 1 is that the artificial wetland system of Comparative Example 3 does not have a benthic animal layer 3, and the sewage entering the artificial wetland system of Comparative Example 3 does not contain antibiotics.

[0082] When HRT=3 days in Example 1, Comparative Example 1, Comparative Example 2 and Comparative Example 3, NH4+ -N, NO x - -N, COD and TN removal rates (Table 1) and antibiotic removal rates (Table 2). The experimental period was 120 days, and the test was performed every 3 days.

[0083] Table 1 NH4 in the system + -N, NO x - -N, COD and TN removal rates

[0084]

[0085] Table 2 Removal rates of three antibiotics in the system

[0086]

[0087] It can be seen from Table 1 that compared with Comparative Example 1, the NH4 + -N, NO x - The average removal rates of -N, COD and TN were increased by 25.65%, 50.11%, 32.91% and 28.53% respectively; compared with Comparative Example 3, the NH4 + -N, NO x - The average removal rates of -N, COD and TN were increased by 9.45%, 22.41%, 15.47% and 5.77% respectively; at the same time, compared with comparative example 3, the NH4 + -N, NO x - The average removal rates of -N, COD and TN were reduced by 12.89%, 18.46%, 13.12% and 17.71% respectively; compared with Comparative Example 2, the NH4 + -N, NO x - The average removal rates of -N, COD and TN were reduced by 8.56%, 7.07%, 6.46% and 13.24% respectively. In addition, it can be seen from Table 2 that compared with Comparative Example 1, the average removal rates of three antibiotics in sewage OTC, CIP and SM2 in Example 1 were increased by 13.83%, 9.87% and 6.79% respectively. The results show that the antibiotics in sewage significantly reduce the removal of NH4 + -N, NO x - -N, COD and TN purification effects. However, the introduction of Earthworms can effectively alleviate this inhibitory effect, which is inseparable from the fact that Earthworms reduce the antibiotic content in the system through various mechanisms.

[0088] In summary, the present invention optimizes the performance of the artificial wetland system by introducing earthworms, combining batch addition and sequencing batch upward inflow and overflow outflow. Earthworms initially degrade antibiotics through bioturbation, improve the wetland environment and increase subsequent survival rates. The upward inflow prevents blockage and replenishes dissolved oxygen, while the overflow outflow prolongs the sewage retention time, promotes the adsorption and degradation of pollutants, and realizes the alternation of aerobic and anaerobic conditions, thereby improving the decontamination efficiency. Earthworms also optimize the microbial community, activate plant functions, enhance the synergistic effect of the matrix-animal-plant-microorganism, and ensure efficient purification performance.

[0089] It is easy for those skilled in the art to understand that, under the premise of no conflict, the above-mentioned advantageous methods can be freely combined and superimposed. The above are only preferred embodiments of the present application and are not intended to limit the present application. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present application should be included in the protection scope of the present application. The above are only preferred implementation methods of the present application. It should be pointed out that for ordinary technicians in this technical field, several improvements and variations can be made without departing from the technical principles of the present application, and these improvements and variations should also be regarded as the protection scope of the present application.

Claims

1. A tremuloides-enhanced purification artificial wetland for alleviating antibiotic stress, characterized in that: include: stromal layer; The matrix layer comprises, from top to bottom, a soil layer (2), a first gravel layer (5) and a second gravel layer (6), wherein the gravel particle size of the first gravel layer (5) is smaller than the gravel particle size of the second gravel layer (6); A wetland plant layer (1); the wetland plant layer (1) is planted on the soil layer (2); A benthic animal layer (3); the benthic animal layer (3) is added into the soil layer (2), and the benthic animals in the benthic animal layer (3) are earthworms; A drainage outlet (7); the drainage outlet (7) is located above the soil layer (2); A water inlet (8); the water inlet (8) is located at the bottom of the second gravel layer (6), and the water inlet (8) is connected to a sewage storage device (9) containing antibiotics.

2. The Earthworm enhanced purification artificial wetland for alleviating antibiotic stress according to claim 1, characterized in that: The artificial wetland also includes: Anti-clogging net (4); the anti-clogging net (4) is arranged at two locations, one of which is arranged at the front end of the drainage outlet (7), and the other is arranged between the soil layer (2) and the first gravel layer (5).

3. The Earthworm enhanced purification artificial wetland for alleviating antibiotic stress according to claim 1, characterized in that: The wetland plant (1) is Iris calamus, and the transplanting density of Iris calamus is 50 to 60 plants / m 2 .

4. The Earthworm enhanced purification artificial wetland for alleviating antibiotic stress according to claim 1, characterized in that: The density of adding earthworms is 1.25×10 4 ~1.5×10 4 pcs / m 2 .

5. The Earthworm enhanced purification artificial wetland for alleviating antibiotic stress according to claim 1, characterized in that: The height of the soil layer (2) is 8 to 12 cm; The gravel particle size of the first gravel layer (5) is 0.9 to 1.6 cm, and the height of the first gravel layer (5) is 5 to 6 cm; The gravel particle size of the second gravel layer (6) is 1.6 to 3.0 cm, and the height of the second gravel layer (6) is 28 to 32 cm.

6. A method for constructing a quasicorn reinforced purification artificial wetland for alleviating antibiotic stress, characterized in that: The method for constructing an artificial wetland according to any one of claims 1 to 5, comprising: Filling the matrix layer, including filling the second gravel layer (6), the first gravel layer (5) and the soil layer (2) in order from bottom to top; Planting wetland plants: Planting wetland plants on the soil layer (2); Add benthic animals and add earthworms to the soil layer (2) to alleviate antibiotic stress; The inlet and outlet water are configured by adopting the method of sequential batch upward inlet and overflow outlet, and the inlet water is sewage containing antibiotics.

7. The method for constructing a terrestris-enhanced purification artificial wetland for alleviating antibiotic stress according to claim 6, characterized in that: The wetland plant is Iris calamus. The Iris calamus transplanting method is: select Iris calamus with a height of 18 to 30 cm and similar leaf numbers and leaf sizes, pre-cultivate for 6 to 8 days, and transplant after the growth is stable. The transplanting density is 50 to 60 plants / m 2 .

8. The method for constructing a terrestris-enhanced purification artificial wetland for alleviating antibiotic stress according to claim 6, characterized in that: The method of adding earthworms to the soil layer (2) comprises: adding earthworms in a batch manner, adding earthworms at 0 day, 30±2 days, 60±2 days and 90±2 days respectively, and adding earthworms at a density of 1.25×10 4 ~1.5×10 4 pcs / m 2 .

9. The method for constructing a terrestris-enhanced purification artificial wetland for alleviating antibiotic stress according to claim 6, characterized in that: After the water flows upward, the hydraulic retention time is 2 to 4 days.

10. The method for constructing a terrestris-enhanced purification artificial wetland for alleviating antibiotic stress according to claim 6, characterized in that: When the concentrations of oxytetracycline, sulfadimethoxine and ciprofloxacin in the sewage containing antibiotics are all 100 μg / L, the artificial wetland according to any one of claims 1 to 5 has a lower concentration of NH4 + -N average removal rate increased by more than 25%, NO x - The average removal rate of -N increased by more than 50%, the average removal rate of COD increased by more than 32%, the average removal rate of TN increased by more than 28%, the average removal rate of oxytetracycline increased by more than 13%, the average removal rate of ciprofloxacin increased by more than 9%, and the average removal rate of sulfamethazine increased by more than 6%.

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