Composite plant growth regulator and preparation method thereof

Abstract

The invention relates to the technical field of growth regulators, in particular to a composite plant growth regulator and a preparation method thereof.The composite plant growth regulator is prepared from, by weight, 0.3-0.8 part of 14-hydroxybrassinosterol, 1-3 parts of thidiazuron, 2-4 parts of triacontanol, 5-8 parts of seaweed fertilizer, 1-3 parts of amino acid, 1-3 parts of fulvic acid, 20-30 parts of degradable hydrogel and 5-8 parts of porous adsorption particles; the compound plant growth regulator is prepared by matching various preparation raw materials and adopting a specific method, and the prepared compound plant growth regulator not only slows down the release speed and effectively regulates the growth of plants for a long time, but also can improve the comprehensive properties such as fertility increasing, air permeability and water retention of soil and promote the growth and development of crops.

Description

Technical Field

[0001] The present application relates to the technical field of growth regulators, and in particular to a composite plant growth regulator and a preparation method thereof. Background Art

[0002] Plant growth regulators are a class of chemical substances that have a regulatory effect on the growth and development of plants. They have chemical properties and physiological effects similar to those of plant hormones. Their presence can affect and effectively regulate the growth and development of plants, including a series of plant life processes from cell growth and division to rooting, germination, flowering, fruiting, maturity and shedding.

[0003] General plant growth regulators are released quickly after application and can regulate plant growth and development in a short period of time, but they are not effective for a long time and have limited effect on regulating crop growth. After they become ineffective, they need to be applied multiple times at appropriate times, which is not only cumbersome to apply but also easily leads to waste of plant growth regulators.

[0004] Hydrogel encapsulation of plant growth regulators can reduce the release rate of plant growth regulators and has potential for application in agricultural production. However, existing hydrogels are usually synthetic polymers that are not easily degraded by microorganisms. Large-scale use will affect plant growth, reduce soil aeration and permeability, and cause environmental pollution. Summary of the invention

[0005] The present application aims to overcome at least one of the defects of the prior art and provide a composite plant growth regulator and a preparation method thereof. The composite plant growth regulator prepared by combining a variety of preparation raw materials and adopting a specific method not only slows down the release rate and effectively regulates plant growth in the long term, but also improves the comprehensive properties of the soil such as fertility, air permeability, and water retention, thereby promoting the growth and development of crops.

[0006] In a first aspect, the present application provides a composite plant growth regulator, which is implemented by the following technical solutions:

[0007] A composite plant growth regulator comprises the following raw materials in parts by weight:

[0008] 0.3-0.8 parts of 14-hydroxybrassinosteroid, 1-3 parts of thidiazuron, 2-4 parts of triacontanol, 5-8 parts of seaweed fertilizer, 1-3 parts of amino acids, 1-3 parts of fulvic acid, 20-30 parts of degradable hydrogel, and 5-8 parts of porous adsorption particles;

[0009] The preparation method of the degradable hydrogel comprises the following steps: firstly, according to weight parts, 8-12 parts of sulfonated modified lignin and 7-9 parts of chitosan are dissolved in a calcium chloride solution with a solute content of 12-15 parts, and heated and stirred at 55-65° C. for 15-25 minutes until completely dissolved; after cooling to room temperature, 4-5 parts of acrylic acid and 0.8-1.2 parts of ammonium persulfate are added, nitrogen is bubbled and stirred for 4-8 minutes, and finally 0.3-0.5 parts of N,N'-methylenebisacrylamide are added and stirred until completely dissolved to obtain a prepolymer solution, and then the prepolymer solution is quickly transferred to a polytetrafluoroethylene mold, and placed in an oven at 65-75° C. for crosslinking for 1.5-2.5 hours to obtain a degradable hydrogel.

[0010] The composite plant growth regulator according to the embodiment of the present application has at least the following beneficial effects:

[0011] This application uses sulfonated modified lignin as the matrix and chitosan as the 2+ The connecting units are connected by ionic bonds, and the sulfonated modified lignin and chitosan are connected by chemical cross-linking to form a sulfonated modified lignin-based double network hydrogel, i.e., a degradable hydrogel. The degradable hydrogel has a denser and richer network structure, which gives less water loss. The addition of chitosan and Ca 2+ Both can improve the water retention properties of hydrogels and can be used to improve the water utilization rate and drought resistance of plants; calcium ions are an important component of plant cell walls and are essential for maintaining the stability of cell structure. They also participate in various signal transduction processes in plants and have an important influence on plant growth and development. Adding an appropriate amount of calcium ions to plant growth regulators can enhance the plant's stress resistance and improve yield and quality.

[0012] The lignin and chitosan in the degradable hydrogel of the present application are both derived from renewable resources, and both lignin and chitosan are biodegradable, so the degradable hydrogel has excellent environmental performance. Lignin has abundant active functional groups such as phenolic hydroxyl groups, which give it strong chemical reactivity and bonding properties. After sulfonation modification, the dispersibility and compatibility of lignin in polylactic acid emulsion are improved. After the hydrogel is applied to the plant, its functional groups in the soil can aggregate and bond soil particles through electrostatic adsorption to form large-size soil particles with a granular structure, effectively improving the soil structure, enhancing the soil particles' resistance to erosion, disintegration and water stability, and improving water retention and soil consolidation.

[0013] The present application combines porous adsorption particles with 14-hydroxybrassinosteroid, thiadiazole, triacontanol, seaweed fertilizer, amino acids, fulvic acid and other raw materials, so that some effective ingredients are adsorbed inside the porous adsorption particles, which can slow down the release rate of plant growth regulators and fertilizers. The raw materials are coordinated and promoted with each other to give full play to the efficacy of each raw material, improve fertility, promote plant growth and development, and can also absorb microorganisms after entering the soil, improve soil structure, increase soil permeability, and facilitate the respiration and growth of plant roots.

[0014] The 14-hydroxybrassinosteroid of the present application has physiological effects such as promoting cell elongation and division, regulating leaf shape, changing cell membrane potential and enzyme activity, and enhancing photosynthesis, thereby significantly improving the growth rate and growth quality of plants. It can also stimulate the activities of various immune enzymes in the plant body and activate the immune system, thereby enhancing the plant's drought resistance, high temperature resistance, frost resistance and other stress resistance capabilities; thiadiazole has extremely strong cell division activity, can delay plant aging, enhance its stress resistance, promote plant photosynthesis, increase crop yield, and improve product quality; triacontanol can increase the effective tillering of crops, promote the healthy growth of seedlings, and can also enhance the respiration of crop plants and promote the absorption and utilization of mineral nutrients by the root system. Using triacontanol for foliar spraying during the initial flowering period and the peak flowering period of crops can promote the formation of flower buds, increase flowering pollination and fruit setting rates, and can also promote flower bud differentiation of fruit trees, increase fruit setting rates, and increase fruit setting rates, thereby achieving the purpose of increasing production.

[0015] The seaweed fertilizer of the present application refers to a biological fertilizer produced from seaweed or seaweed extracts through fermentation, acid-base process or fertilizer mixing process. Seaweed fertilizer is rich in nutrients and contains various minerals such as potassium, calcium, magnesium, iron, zinc, etc. The seaweed polysaccharides, alginate, highly unsaturated fatty acids and various natural plant growth regulators unique to seaweed can stimulate the production of nonspecific active factors in plants and regulate the balance of endogenous factors. It can also improve soil, enhance crop photosynthesis and enhance crop stress resistance.

[0016] The fulvic acid of the present application has a small molecular weight and is easily absorbed and utilized by organisms. It can reduce the opening of crop stomata, reduce water transpiration, and increase the activity of various enzymes and chlorophyll content, increase metabolism and photosynthesis, and improve crop resistance to stress such as frost and disease, increase crop yield, improve crop quality, and can also chelate trace elements to improve the crop's ability to absorb trace elements.

[0017] According to some embodiments of the present application, the method for preparing the porous adsorption particles comprises the following steps:

[0018] S1. The rapeseed pollen, Fe3O4 powder, and nano zinc oxide were added to an aqueous solution of a silane coupling agent, ultrasonically stirred, and then dried and ground to form a composite powder;

[0019] S2 weighed 30-40 parts by weight of clay, 4-8 parts by weight of ammonium bicarbonate, 35-50 parts by weight of the composite powder, mixed evenly, added 25-35 parts by weight of water, granulated to form composite particles;

[0020] S3. The composite particles are preheated to 105-115°C, then calcined at 500-700°C, cooled, and ground to form porous adsorption particles.

[0021] Rapeseed pollen is abundant in nature, has good biocompatibility, is easily degraded in the later stage, and has strong adsorption properties in the porous material after sintering. Nano zinc oxide, as a nanomaterial, has the characteristics of high biological activity, high absorption rate, strong antioxidant capacity, safety and stability. Adding nano zinc oxide to porous adsorption particles can supplement the plant's demand for zinc, participate in various metabolic processes, including protein synthesis, carbohydrate metabolism and redox reactions, improve the plant's stress resistance, promote root development and nutrient absorption, and thus promote plant growth and development.

[0022] Furthermore, in step S1, the weight ratio of the rapeseed pollen to the Fe3O4 powder and the nano zinc oxide is (1-3):1:1.

[0023] Furthermore, the silane coupling agent in step S1 is γ-aminopropyltriethoxysilane.

[0024] Furthermore, after grinding in step S3, the step also includes pressurized spraying with sodium hexadecyl diphenyl oxide disulfonate. In the molecular structure of sodium hexadecyl diphenyl oxide disulfonate, the long alkyl chain provides hydrophobicity, the part where two benzene rings are connected by an ether bond increases the stability and compatibility of the molecule, and the sulfonic acid group provides hydrophilicity and ionicity, enhancing solubility in water. This molecular structure enables sodium hexadecyl diphenyl oxide disulfonate to have excellent dispersibility and can effectively disperse porous adsorption particles in a liquid medium.

[0025] Furthermore, the amount of the sodium hexadecyl diphenyl oxide disulfonate is 1-2 wt % of the weight of the porous adsorption particles.

[0026] According to some embodiments of the present application, the particle size of the porous adsorption particles is 1-3 μm.

[0027] According to some embodiments of the present application, the method for preparing the sulfonated modified lignin comprises the following steps:

[0028] The lignin and Na2SO3 are uniformly mixed in a weight ratio of (3-5):(2-3), and a NaOH solution with a mass fraction of 15% is added, and the mixture is reacted at a temperature of 150-200°C for 3-5 hours to obtain sulfonated modified lignin.

[0029] The reaction temperature, reaction time and amount of Na2SO3 used in the preparation of sulfonated modified lignin are carefully set. For example, too high a reaction temperature may lead to lignin degradation, while too much Na2SO3 may increase the cost and reduce the purity of the product.

[0030] According to some embodiments of the present application, the amino acid is selected from at least one of alanine, arginine, aspartic acid, cysteine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine.

[0031] In a second aspect, the present application provides a method for preparing the above-mentioned composite plant growth regulator, which is achieved by the following technical scheme:

[0032] The preparation method of the composite plant growth regulator comprises the following steps:

[0033] According to weight proportions, 14-hydroxybrassinosteroid, thidiazuron, triacontanol, seaweed fertilizer, amino acid and fulvic acid are slowly poured into the degradable hydrogel in a light-proof environment, the degradable hydrogel is stirred at a speed of 80-100 r / min while pouring, porous adsorption particles are added, and the composite plant growth regulator is obtained after uniform mixing.

[0034] The preparation method of the composite plant growth regulator according to the embodiment of the present application has at least the following beneficial effects:

[0035] The preparation method of the present application has simple steps. By fully mixing 14-hydroxybrassinosteroid, thiadiazole, triacontanol, seaweed fertilizer, amino acids, fulvic acid, porous adsorption particles and degradable hydrogel, some effective ingredients are adsorbed into the porous adsorption particles, which can slow down the release rate of plant growth regulators and fertilizers. After the composite plant growth regulator is sprayed onto the plant surface, due to the effect of the degradable hydrogel, the release rate of the plant growth regulator can be further slowed down, promoting plant growth for a long time, and storing part of the water, reducing the evaporation of water on the plant surface, and replenishing the water required for plant growth. At the same time, the preparation method of the present application does not require the use of complex production equipment, has low cost, is pollution-free in the manufacturing process, does not emit toxic substances, does not harm the health of operators, and is suitable for industrial production. DETAILED DESCRIPTION

[0036] To make the purpose, technical solution and advantages of the present application clearer, the following will be further described in detail in conjunction with specific embodiments. The embodiments described here are only part of the embodiments of the present application and cannot be understood as limiting the scope of protection of the present application.

[0037] Example 1

[0038] Preparation of composite plant growth regulators:

[0039] (1) Preparation of sulfonated modified lignin:

[0040] The lignin and Na2SO3 were mixed evenly at a weight ratio of 4:2.5, and a 15% by mass NaOH solution was added, and the mixture was reacted at a temperature of 180°C for 4 hours to obtain sulfonated modified lignin;

[0041] (2) Preparation of degradable hydrogel: First, 10 parts of sulfonated modified lignin and 8 parts of chitosan were dissolved in a calcium chloride solution with a solute content of 13 parts according to weight, and heated and stirred at 60°C for 20 minutes until completely dissolved; after cooling to room temperature, 4 parts of acrylic acid and 1 part of ammonium persulfate were added, nitrogen was bubbled and stirred for 6 minutes, and finally 0.4 parts of N,N'-methylenebisacrylamide was added and stirred until completely dissolved to obtain a prepolymer solution, and then the prepolymer solution was quickly transferred to a polytetrafluoroethylene mold, placed in an oven at 70°C for crosslinking for 2 hours to obtain a degradable hydrogel;

[0042] (3) Preparation of porous adsorption particles:

[0043] S1. Rapeseed pollen, Fe3O4 powder and nano zinc oxide were added to an aqueous solution of γ-aminopropyltriethoxysilane in a weight ratio of 2:1:1, ultrasonicated and stirred, and then dried and ground to form a composite powder;

[0044] S2 weighed 35 parts by weight of clay, 6 parts by weight of ammonium bicarbonate, 45 parts by weight of the composite powder, mixed evenly, added 30 parts by weight of water, granulated to form composite particles;

[0045] S3. The composite particles are preheated to 110°C, calcined at 600°C, cooled, ground, and pressurized sprayed with sodium hexadecyl diphenyl ether disulfonate to produce porous adsorption particles having a particle size of 1-3 μm;

[0046] The amount of sodium hexadecyl diphenyl ether disulfonate is 1.5wt% of the weight of the porous adsorption particles;

[0047] (4) Slowly pour 0.5 parts of 14-hydroxybrassinosteroid, 2 parts of thidiazuron, 3 parts of triacontanol, 6.5 parts of seaweed fertilizer, 2 parts of alanine, and 2 parts of fulvic acid into the degradable hydrogel in a light-proof environment, stirring 25 parts of the degradable hydrogel at a speed of 90 r / min while pouring, add 6 parts of porous adsorption particles, and mix well to obtain a composite plant growth regulator.

[0048] Example 2

[0049] Preparation of composite plant growth regulators:

[0050] (1) Preparation of sulfonated modified lignin:

[0051] The lignin and Na2SO3 were mixed uniformly at a weight ratio of 5:2, and a NaOH solution with a mass fraction of 15% was added, and the mixture was reacted at a temperature of 200°C for 3 hours to obtain sulfonated modified lignin;

[0052] (2) Preparation of degradable hydrogel: First, 12 parts of sulfonated modified lignin and 7 parts of chitosan were dissolved in a calcium chloride solution with a solute content of 15 parts according to weight, and heated and stirred at 55°C for 25 minutes until completely dissolved; after cooling to room temperature, 4 parts of acrylic acid and 1.2 parts of ammonium persulfate were added, nitrogen was bubbled and stirred for 4 minutes, and finally 0.5 parts of N,N'-methylenebisacrylamide was added and stirred until completely dissolved to obtain a prepolymer solution, and then the prepolymer solution was quickly transferred to a polytetrafluoroethylene mold, placed in an oven at 65°C for crosslinking for 2.5 hours to obtain a degradable hydrogel;

[0053] (3) Preparation of porous adsorption particles:

[0054] S1. Rapeseed pollen, Fe3O4 powder and nano zinc oxide were added to an aqueous solution of γ-aminopropyltriethoxysilane in a weight ratio of 1:1:1, ultrasonicated and stirred, and then dried and ground to form a composite powder;

[0055] S2 weighed 40 parts by weight of clay, 4 parts by weight of ammonium bicarbonate, 50 parts by weight of the composite powder, mixed evenly, added 25 parts by weight of water, granulated to form composite particles;

[0056] S3. The composite particles are preheated to 115°C, calcined at 500°C, cooled, ground, and pressurized sprayed with sodium hexadecyl diphenyl ether disulfonate to produce porous adsorption particles having a particle size of 1-3 μm;

[0057] The amount of sodium hexadecyl diphenyl ether disulfonate is 2wt% of the weight of the porous adsorption particles;

[0058] (4) Slowly pour 0.3 parts of 14-hydroxybrassinosteroid, 3 parts of thiadiazole, 2 parts of triacontanol, 8 parts of seaweed fertilizer, 1 part of glutamic acid, and 3 parts of fulvic acid into the degradable hydrogel in a light-proof environment, stir 30 parts of the degradable hydrogel at a speed of 80 r / min while pouring, add 5 parts of porous adsorption particles, and mix well to obtain a composite plant growth regulator.

[0059] Example 3

[0060] Preparation of composite plant growth regulators:

[0061] (1) Preparation of sulfonated modified lignin:

[0062] The lignin and Na2SO3 were mixed uniformly at a weight ratio of 3:3, and a 15% by mass NaOH solution was added, and the mixture was reacted at a temperature of 150°C for 5 hours to obtain sulfonated modified lignin;

[0063] (2) Preparation of degradable hydrogel: First, 8 parts of sulfonated modified lignin and 9 parts of chitosan were dissolved in a calcium chloride solution with a solute content of 12 parts according to weight, and heated and stirred at 65°C for 15 minutes until completely dissolved; after cooling to room temperature, 5 parts of acrylic acid and 0.8 parts of ammonium persulfate were added, nitrogen was bubbled and stirred for 8 minutes, and finally 0.3 parts of N,N'-methylenebisacrylamide was added and stirred until completely dissolved to obtain a prepolymer solution, and then the prepolymer solution was quickly transferred to a polytetrafluoroethylene mold, placed in an oven at 75°C for crosslinking for 1.5 hours to obtain a degradable hydrogel;

[0064] (3) Preparation of porous adsorption particles:

[0065] S1. Rapeseed pollen, Fe3O4 powder and nano zinc oxide were added to an aqueous solution of γ-aminopropyltriethoxysilane in a weight ratio of 3:1:1, ultrasonicated and stirred, and then dried and ground to form a composite powder;

[0066] S2 weighed 30 parts by weight of clay, 8 parts by weight of ammonium bicarbonate, 35 parts by weight of the composite powder, mixed evenly, added 35 parts by weight of water, granulated to form composite particles;

[0067] S3. The composite particles are preheated to 105°C, calcined at 700°C, cooled, ground, and pressurized sprayed with sodium hexadecyl diphenyl ether disulfonate to produce porous adsorption particles having a particle size of 1-3 μm;

[0068] The amount of sodium hexadecyl diphenyl ether disulfonate is 1wt% of the weight of the porous adsorption particles;

[0069] (4) Slowly pour 0.8 parts of 14-hydroxybrassinosteroid, 1 part of thidiazuron, 4 parts of triacontanol, 5 parts of seaweed fertilizer, 1 part of glycine, 1 part of lysine, 1 part of phenylalanine, and 1 part of fulvic acid into the degradable hydrogel in a light-proof environment, while stirring 20 parts of the degradable hydrogel at a speed of 100 r / min, adding 8 parts of porous adsorption particles, and mixing well to obtain a composite plant growth regulator.

[0070] Example 4

[0071] Preparation of composite plant growth regulators:

[0072] (1) Preparation of sulfonated modified lignin:

[0073] The lignin and Na2SO3 were mixed uniformly at a weight ratio of 4:3, and a 15% by mass NaOH solution was added, and the mixture was reacted at a temperature of 170°C for 4 hours to obtain sulfonated modified lignin;

[0074] (2) Preparation of degradable hydrogel: First, 10 parts of sulfonated modified lignin and 8 parts of chitosan were dissolved in a calcium chloride solution with a solute content of 13 parts according to weight, and heated and stirred at 60°C for 20 minutes until completely dissolved; after cooling to room temperature, 5 parts of acrylic acid and 1 part of ammonium persulfate were added, nitrogen was bubbled and stirred for 6 minutes, and finally 0.4 parts of N,N'-methylenebisacrylamide was added and stirred until completely dissolved to obtain a prepolymer solution, and then the prepolymer solution was quickly transferred to a polytetrafluoroethylene mold, placed in an oven at 70°C for crosslinking for 2 hours to obtain a degradable hydrogel;

[0075] (3) Preparation of porous adsorption particles:

[0076] S1. Rapeseed pollen, Fe3O4 powder and nano zinc oxide were added to an aqueous solution of γ-aminopropyltriethoxysilane in a weight ratio of 2:1:1, ultrasonicated and stirred, and then dried and ground to form a composite powder;

[0077] S2 weighed 35 parts by weight of clay, 5 parts by weight of ammonium bicarbonate, 40 parts by weight of the composite powder, mixed evenly, added 30 parts by weight of water, granulated to form composite particles;

[0078] S3. The composite particles are preheated to 110°C, calcined at 600°C, cooled, ground, and pressurized sprayed with sodium hexadecyl diphenyl ether disulfonate to produce porous adsorption particles having a particle size of 1-3 μm;

[0079] The amount of sodium hexadecyl diphenyl ether disulfonate is 1.5wt% of the weight of the porous adsorption particles;

[0080] (4) According to weight portions, 0.6 parts of 14-hydroxybrassinosteroid, 2 parts of thidiazuron, 3 parts of triacontanol, 7 parts of seaweed fertilizer, 2 parts of tyrosine, and 1 part of fulvic acid are slowly poured into the degradable hydrogel in a light-proof environment, and 25 parts of the degradable hydrogel is stirred at a speed of 900 r / min while pouring, and 6 parts of porous adsorption particles are added, and the composite plant growth regulator is obtained after mixing evenly.

[0081] Comparative Example 1

[0082] Preparation of composite plant growth regulators:

[0083] (1) Preparation of sulfonated modified lignin:

[0084] The lignin and Na2SO3 were mixed evenly at a weight ratio of 4:2.5, and a 15% by mass NaOH solution was added, and the mixture was reacted at a temperature of 180°C for 4 hours to obtain sulfonated modified lignin;

[0085] (2) Preparation of degradable hydrogel: First, 10 parts of sulfonated modified lignin and 8 parts of chitosan were dissolved in water according to weight, and heated and stirred at 60°C for 20 minutes until completely dissolved; after cooling to room temperature, 4 parts of acrylic acid and 1 part of ammonium persulfate were added, nitrogen was bubbled and stirred for 6 minutes, and finally 0.4 parts of N,N'-methylenebisacrylamide was added and stirred until completely dissolved to obtain a prepolymer solution, and then the prepolymer solution was quickly transferred to a polytetrafluoroethylene mold, placed in an oven at 70°C for crosslinking for 2 hours to obtain a degradable hydrogel;

[0086] (3) Preparation of porous adsorption particles:

[0087] S1. Rapeseed pollen, Fe3O4 powder and nano zinc oxide were added to an aqueous solution of γ-aminopropyltriethoxysilane in a weight ratio of 2:1:1, ultrasonicated and stirred, and then dried and ground to form a composite powder;

[0088] S2 weighed 35 parts by weight of clay, 6 parts by weight of ammonium bicarbonate, 45 parts by weight of the composite powder, mixed evenly, added 30 parts by weight of water, granulated to form composite particles;

[0089] S3. The composite particles are preheated to 110°C, calcined at 600°C, cooled, ground, and pressurized sprayed with sodium hexadecyl diphenyl ether disulfonate to produce porous adsorption particles having a particle size of 1-3 μm;

[0090] The amount of sodium hexadecyl diphenyl ether disulfonate is 1.5wt% of the weight of the porous adsorption particles;

[0091] (4) Slowly pour 0.5 parts of 14-hydroxybrassinosteroid, 2 parts of thidiazuron, 3 parts of triacontanol, 6.5 parts of seaweed fertilizer, 2 parts of alanine, and 2 parts of fulvic acid into the degradable hydrogel in a light-proof environment, stirring 25 parts of the degradable hydrogel at a speed of 90 r / min while pouring, add 6 parts of porous adsorption particles, and mix well to obtain a composite plant growth regulator.

[0092] Comparative Example 2

[0093] Preparation of composite plant growth regulators:

[0094] (1) Preparation of degradable hydrogel: First, 10 parts of lignin and 8 parts of chitosan were dissolved in a calcium chloride solution with a solute content of 13 parts according to weight, and heated and stirred at 60°C for 20 minutes until completely dissolved; after cooling to room temperature, 4 parts of acrylic acid and 1 part of ammonium persulfate were added, nitrogen was bubbled and stirred for 6 minutes, and finally 0.4 parts of N,N'-methylenebisacrylamide was added and stirred until completely dissolved to obtain a prepolymer solution, and then the prepolymer solution was quickly transferred to a polytetrafluoroethylene mold, and placed in an oven at 70°C for crosslinking for 2 hours to obtain a degradable hydrogel;

[0095] (2) Preparation of porous adsorption particles:

[0096] S1. Rapeseed pollen, Fe3O4 powder and nano zinc oxide were added to an aqueous solution of γ-aminopropyltriethoxysilane in a weight ratio of 2:1:1, ultrasonicated and stirred, and then dried and ground to form a composite powder;

[0097] S2 weighed 35 parts by weight of clay, 6 parts by weight of ammonium bicarbonate, 45 parts by weight of the composite powder, mixed evenly, added 30 parts by weight of water, granulated to form composite particles;

[0098] S3. The composite particles are preheated to 110°C, calcined at 600°C, cooled, ground, and pressurized sprayed with sodium hexadecyl diphenyl ether disulfonate to produce porous adsorption particles having a particle size of 1-3 μm;

[0099] The amount of sodium hexadecyl diphenyl ether disulfonate is 1.5wt% of the weight of the porous adsorption particles;

[0100] (3) According to weight portions, 0.5 parts of 14-hydroxybrassinosteroid, 2 parts of thiadiazole, 3 parts of triacontanol, 6.5 parts of seaweed fertilizer, 2 parts of alanine, and 2 parts of fulvic acid are slowly poured into the degradable hydrogel in a light-proof environment, and 25 parts of the degradable hydrogel is stirred at a speed of 90 r / min while pouring, and 6 parts of porous adsorption particles are added, and the composite plant growth regulator is obtained after mixing evenly.

[0101] Comparative Example 3

[0102] Preparation of composite plant growth regulators:

[0103] (1) Preparation of sulfonated modified lignin:

[0104] The lignin and Na2SO3 were mixed evenly at a weight ratio of 4:2.5, and a 15% by mass NaOH solution was added, and the mixture was reacted at a temperature of 180°C for 4 hours to obtain sulfonated modified lignin;

[0105] (2) Preparation of degradable hydrogel: First, 10 parts of sulfonated modified lignin and 8 parts of chitosan were dissolved in a calcium chloride solution with a solute content of 13 parts according to weight, and heated and stirred at 60°C for 20 minutes until completely dissolved; after cooling to room temperature, 4 parts of acrylic acid and 1 part of ammonium persulfate were added, nitrogen was bubbled and stirred for 6 minutes, and finally 0.4 parts of N,N'-methylenebisacrylamide was added and stirred until completely dissolved to obtain a prepolymer solution, and then the prepolymer solution was quickly transferred to a polytetrafluoroethylene mold, placed in an oven at 70°C for crosslinking for 2 hours to obtain a degradable hydrogel;

[0106] (3) According to weight portions, 0.5 parts of 14-hydroxybrassinosteroid, 2 parts of thidiazuron, 3 parts of triacontanol, 6.5 parts of seaweed fertilizer, 2 parts of alanine, and 2 parts of fulvic acid are slowly poured into the degradable hydrogel in a light-proof environment, and 25 parts of the degradable hydrogel is stirred at a speed of 90 r / min while pouring, and the composite plant growth regulator is obtained after mixing evenly.

[0107] Comparative Example 4

[0108] Preparation of composite plant growth regulators:

[0109] (1) Preparation of sulfonated modified lignin:

[0110] The lignin and Na2SO3 were mixed evenly at a weight ratio of 4:2.5, and a 15% by mass NaOH solution was added, and the mixture was reacted at a temperature of 180°C for 4 hours to obtain sulfonated modified lignin;

[0111] (2) Preparation of degradable hydrogel: First, 10 parts of sulfonated modified lignin and 8 parts of chitosan were dissolved in a calcium chloride solution with a solute content of 13 parts according to weight, and heated and stirred at 60°C for 20 minutes until completely dissolved; after cooling to room temperature, 4 parts of acrylic acid and 1 part of ammonium persulfate were added, nitrogen was bubbled and stirred for 6 minutes, and finally 0.4 parts of N,N'-methylenebisacrylamide was added and stirred until completely dissolved to obtain a prepolymer solution, and then the prepolymer solution was quickly transferred to a polytetrafluoroethylene mold, placed in an oven at 70°C for crosslinking for 2 hours to obtain a degradable hydrogel;

[0112] (3) Preparation of porous adsorption particles:

[0113] S1. Rapeseed pollen, Fe3O4 powder and nano zinc oxide were added to an aqueous solution of γ-aminopropyltriethoxysilane in a weight ratio of 2:1:1, ultrasonicated and stirred, and then dried and ground to form a composite powder;

[0114] S2 weighed 35 parts by weight of clay, 6 parts by weight of ammonium bicarbonate, 45 parts by weight of the composite powder, mixed evenly, added 30 parts by weight of water, granulated to form composite particles;

[0115] S3. The composite particles are preheated to 110°C, calcined at 600°C, cooled, ground, and pressurized sprayed with sodium hexadecyl diphenyl ether disulfonate to produce porous adsorption particles having a particle size of 1-3 μm;

[0116] The amount of sodium hexadecyl diphenyl ether disulfonate is 1.5wt% of the weight of the porous adsorption particles;

[0117] (4) Slowly pour 2 parts of thiadiol, 3 parts of triacontanol, 6.5 parts of seaweed fertilizer, 2 parts of alanine, and 2 parts of fulvic acid into the degradable hydrogel in a light-proof environment, stirring 25 parts of the degradable hydrogel at a speed of 90 r / min while pouring, add 6 parts of porous adsorption particles, and mix well to obtain a composite plant growth regulator.

[0118] Experimental example

[0119] Young pea plants with similar growth conditions and a plant height of 5 cm were selected and planted in 8 different farmlands in the same village. 20 pea plants were planted in each farmland. The 8 farmlands were sprayed with the composite plant growth regulators prepared in Examples 1-4 and Comparative Examples 1-4, respectively. The plants were watered once a week, and the soil moisture content was measured before the last watering for 3 weeks. The plant height was tested after the last watering for 3 weeks. The photosynthesis rate of the leaves was tested with a portable photosynthesis meter at 13:00 the next afternoon. The healthy leaves at similar parts of the plants were selected for the test leaves. The measured data of peas planted in each farmland were averaged. The results are shown in Table 1.

[0120] Table 1

[0121]

[0122] It can be seen from Table 1 that the composite plant growth regulators prepared in Examples 1-4 of the present application can promote the growth of pea plants, accelerate the photosynthesis rate of the plants, and increase the moisture content in the soil after being sprayed on pea fields.

[0123] The preparation raw material of the degradable hydrogel in Comparative Example 1 does not use calcium chloride, and the rest is the same as Example 1. The effect of the composite plant growth regulator prepared in Comparative Example 1 is obviously not as good as that in Example 1 of the present application. The plant height and leaf photosynthesis rate of peas are significantly reduced, indicating that the present application uses sulfonated modified lignin as the matrix and chitosan as the matrix to react with Ca 2+ The connecting units are connected by ionic bonds, and the sulfonated modified lignin and chitosan are connected by chemical cross-linking to form a sulfonated modified lignin-based double network hydrogel, i.e., a degradable hydrogel. The degradable hydrogel has a denser and richer network structure, which gives less water loss. The addition of chitosan and Ca 2+Both can improve the water retention properties of hydrogels and can be used to improve the water utilization rate and drought resistance of plants; calcium ions are an important component of plant cell walls and are essential for maintaining the stability of cell structure. They also participate in various signal transduction processes in plants and have an important influence on plant growth and development. Adding an appropriate amount of calcium ions to plant growth regulators can enhance the plant's stress resistance and improve yield and quality.

[0124] The lignin in the raw material of the degradable hydrogel of Comparative Example 2 is sulfonated, and the rest is the same as Example 1. The effect of the composite plant growth regulator prepared in Comparative Example 2 is obviously not as good as that in Example 1 of the present application. The pea plant height, leaf photosynthesis rate and soil moisture content are significantly reduced, indicating that lignin has rich active functional groups such as phenolic hydroxyl groups, which give it strong chemical reactivity and bonding properties. After sulfonation modification, the dispersibility and compatibility of lignin in polylactic acid emulsion are improved. After the hydrogel is applied to the plant, its functional groups in the soil can aggregate and bond soil particles through electrostatic adsorption to form large-size soil particles with a granular structure, effectively improving the soil structure, enhancing the soil particles' resistance to erosion, anti-disintegration and water stability, and improving water retention and soil consolidation.

[0125] Porous adsorption particles were not used in the preparation raw materials of Comparative Example 3, and the rest were the same as Example 1. The effect of the composite plant growth regulator prepared in Comparative Example 3 was obviously not as good as that in Example 1 of the present application. The pea plant height, leaf photosynthesis rate and soil moisture content were significantly reduced, indicating that the present application combines porous adsorption particles with 14-hydroxybrassinosteroid, thiadiazole, triacontanol, seaweed fertilizer, amino acids, fulvic acid and other raw materials, and some effective ingredients are adsorbed into the interior by the porous adsorption particles, which can slow down the release rate of plant growth regulators and fertilizers. The raw materials are coordinated and promoted with each other, and the efficacy of each raw material is fully exerted, the fertility is improved, and the growth and development of plants are promoted. After entering the soil, it can also adsorb microorganisms, improve soil structure, increase soil permeability, and is beneficial to the respiration and growth of plant roots.

[0126] 14-hydroxybrassinosteroid was not used in the preparation raw materials of Comparative Example 4, and the rest were the same as Example 1. The effect of the composite plant growth regulator prepared in Comparative Example 4 was obviously not as good as that in Example 1 of the present application. The plant height and leaf photosynthesis rate of peas were significantly reduced, indicating that the 14-hydroxybrassinosteroid, thiadiazole and triacontanol of the present application are mutually matched and indispensable. 14-hydroxybrassinosteroid has physiological effects such as promoting cell elongation and division, regulating leaf shape, changing cell membrane potential and enzyme activity, and enhancing photosynthesis, thereby significantly improving the growth rate and growth quality of plants. It can also stimulate the activity of various immune enzymes in the plant and activate the immune system, thereby enhancing the plant's drought resistance, high temperature resistance, frost resistance and other stress resistance.

[0127] Although the embodiments of the present application have been shown and described, those skilled in the art will appreciate that various changes, modifications, substitutions or variations may be made to the embodiments without departing from the principles and purpose of the present application, and that the technical solutions after these changes, modifications, substitutions or variations will fall within the scope of protection of the present application.

Claims

1. A composite plant growth regulator, characterized in that: The preparation comprises the following raw materials in parts by weight: 0.3-0.8 parts of 14-hydroxybrassinosteroid, 1-3 parts of thidiazuron, 2-4 parts of triacontanol, 5-8 parts of seaweed fertilizer, 1-3 parts of amino acids, 1-3 parts of fulvic acid, 20-30 parts of degradable hydrogel, and 5-8 parts of porous adsorption particles; The preparation method of the degradable hydrogel comprises the following steps: firstly, dissolving 8-12 parts of sulfonated modified lignin and 7-9 parts of chitosan in a calcium chloride solution with a solute content of 12-15 parts according to weight, and heating and stirring at 55-65° C. for 15-25 minutes until completely dissolved; after cooling to room temperature, adding 4-5 parts of acrylic acid and 0.8-1.2 parts of ammonium persulfate, bubbling nitrogen and stirring for 4-8 minutes, and finally adding 0.3-0.5 parts of N,N'-methylenebisacrylamide and stirring until completely dissolved to obtain a prepolymer solution, and then quickly transferring the prepolymer solution to a polytetrafluoroethylene mold, and placing it in an oven at 65-75° C. for crosslinking for 1.5-2.5 hours to obtain the degradable hydrogel.

2. A composite plant growth regulator according to claim 1, characterized in that: The method for preparing the porous adsorption particles comprises the following steps: S1. The rapeseed pollen, Fe3O4 powder, and nano zinc oxide were added to an aqueous solution of a silane coupling agent, ultrasonically stirred, and then dried and ground to form a composite powder; S2 weighed 30-40 parts by weight of clay, 4-8 parts by weight of ammonium bicarbonate, 35-50 parts by weight of the composite powder, mixed evenly, added 25-35 parts by weight of water, granulated to form composite particles; S3. The composite particles are preheated to 105-115°C, then calcined at 500-700°C, cooled, and ground to form porous adsorption particles.

3. A composite plant growth regulator according to claim 2, characterized in that: In step S1, the weight ratio of the rapeseed pollen to the Fe3O4 powder and the nano zinc oxide is (1-3):1:

1.

4. A composite plant growth regulator according to claim 2, characterized in that: After the grinding in step S3, the process further comprises using sodium hexadecyl diphenyl ether disulfonate for pressurized spraying.

5. A composite plant growth regulator according to claim 4, characterized in that: The amount of sodium hexadecyl diphenyl ether disulfonate used is 1-2 wt % of the weight of the porous adsorption particles.

6. A composite plant growth regulator according to claim 2, characterized in that: The silane coupling agent in step S1 is γ-aminopropyltriethoxysilane.

7. A composite plant growth regulator according to claim 1, characterized in that: The particle size of the porous adsorption particles is 1-3 μm.

8. A composite plant growth regulator according to claim 1, characterized in that: The preparation method of the sulfonated modified lignin comprises the following steps: The lignin and Na2SO3 are uniformly mixed in a weight ratio of (3-5):(2-3), and a NaOH solution with a mass fraction of 15% is added, and the mixture is reacted at a temperature of 150-200°C for 3-5 hours to obtain sulfonated modified lignin.

9. A composite plant growth regulator according to claim 1, characterized in that: The amino acid is selected from at least one of alanine, arginine, aspartic acid, cysteine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine and valine.

10. A method for preparing the composite plant growth regulator according to any one of claims 1 to 9, characterized in that: The following steps are involved: According to weight proportions, 14-hydroxybrassinosteroid, thidiazuron, triacontanol, seaweed fertilizer, amino acid and fulvic acid are slowly poured into the degradable hydrogel in a light-proof environment, the degradable hydrogel is stirred at a speed of 80-100 r / min while pouring, porous adsorption particles are added, and the composite plant growth regulator is obtained after uniform mixing.

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