A plant multi-effect stress-resistant agent and preparation method thereof

Abstract

The invention discloses a plant multi-effect stress-resistant agent and a preparation method thereof, which comprises the following raw materials by weight: 30-40 parts of modified carrier, 20-30 parts of zeolite powder, 5-10 parts of plant ash, 5-7 parts of composite amino acid, 5-10 parts of seaweed extract, 15-25 parts of humic acid, 3-5 parts of betaine, 2-3 parts of rhamnolipid, and 0.5-0.8 parts of trace elements. The plant multi-effect stress-resistant agent is compounded with a variety of raw materials in a scientific ratio, effectively improves the resistance of plants to a variety of adversities, enhances the growth vitality of plants, and promotes the increase of crop yield and quality.

Description

Technical Field

[0001] The invention belongs to the technical field, and particularly relates to a plant multi-effect stress resistance agent and a preparation method thereof. Background Art

[0002] In nature, plants are not always in suitable habitats. Due to differences in climatic conditions and geographical locations, as well as changes in habitats caused by human activities, the plants are often beyond the range of maintaining normal growth and development, which will cause certain damage to the plants and even prevent them from surviving normally. An unfavorable environment will directly inhibit the normal growth and development of plants. We call this environment adversity, also known as stress. The adaptability and resistance of plants to adverse environments are called stress resistance or stress tolerance. Plant stress resistance is the ability of plants to resist adverse external factors, such as drought resistance, salinity resistance, waterlogging resistance, wind resistance, frost resistance, and resistance to diseases and insect pests.

[0003] At present, most stress-resistant agents sold on the market are foliar fertilizers containing plant growth regulators, and are mainly formulated based on the adverse symptoms that crops are prone to, with relatively simple functions. Plant growth regulators can only be used during certain periods of crop growth. Crops are affected by their own internal factors and external factors such as soil, fertilizer, water, climate, pests and diseases, and management during the entire growth process. It is impossible for only one adverse symptom to occur at the same time. Especially in the process of growth, they are susceptible to adverse environments such as low temperature, humidity, drought, pests and diseases. Improper fertilization and pesticide application cause fertilizer damage, pesticide damage and crop nutrient deficiency, which often cause great losses to agricultural production and reduce the yield.

[0004] Chinese patent application CN101597194A discloses a high-nutrient foliar fertilizer for improving plant stress resistance, which comprises mixing and dissolving water, compound amino acids, potassium dihydrogen phosphate, and citric acid, adding alcohol and glutathione for coupling reaction, and then adding Na-EDTA, FeSO 4 、ZnSO 4 、MnSO 4 ,CuSO 4 MgSO 4 , H 3 BO 3 , Vitamin C, Na 2 MoO 4 、KNO 3, urea for mixed reaction, and after the reaction is complete, filter, and the obtained filtrate is the finished product. The invention has a simple preparation process and low cost, can effectively improve the stress resistance of plants, directly promote the growth of plant roots, regulate the physiological functions of plants, and improve the resistance of plants to diseases, drought, cold, etc. It has the characteristics of comprehensive nutrition and good absorption effect, and provides due guarantee for the normal growth of plants, thereby laying a solid material foundation for improving the quality of agricultural products. However, the application specification does not disclose what adverse external factors the stress-resistant agent has stress-resistant effect on. Summary of the invention

[0005] In view of the deficiencies of the prior art, the object of the present invention is to provide a plant multi-effect stress resistance agent and a preparation method thereof.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A plant multi-effect stress-resistant agent, comprising the following raw materials by weight:

[0008] 30-40 parts of modified carrier, 20-30 parts of zeolite powder, 5-10 parts of plant ash, 5-7 parts of complex amino acid, 5-10 parts of seaweed extract, 15-25 parts of humic acid, 3-5 parts of betaine, 2-3 parts of rhamnolipid, and 0.5-0.8 parts of trace elements.

[0009] In the present invention, the multi-effect stress-resistant agent is compounded with a variety of raw materials in a scientific ratio, which effectively improves the resistance of plants to various adversities, enhances the growth vitality of plants, and promotes the increase of crop yield and quality.

[0010] Preferably, a plant multi-effect stress resistance agent comprises the following raw materials by weight:

[0011] 30-40 parts of modified carrier, 20-30 parts of zeolite powder, 5-10 parts of plant ash, 5-7 parts of complex amino acid, 5-10 parts of seaweed extract, 15-25 parts of humic acid, 3-5 parts of betaine, 2-3 parts of rhamnolipid, and 0.5-0.8 parts of trace elements.

[0012] Preferably, the trace elements are composed of iron sulfate, zinc sulfate, manganese sulfate and ammonium molybdate in a mass ratio of 1:0.5-0.6:0.3-0.4:0.2-0.3.

[0013] In the present invention, the added trace elements can supplement the trace elements required for plant growth, promote enzyme activity, enhance plant metabolism, and thus improve the stress resistance and growth and development level of the plant.

[0014] Preferably, the composite amino acid is a mixture of glycine, proline and γ-aminobutyric acid in a mass ratio of 5-6:2-3:2-3.

[0015] In the present invention, the added composite amino acids are composed of glycine, proline and γ-aminobutyric acid, which can be used as plant osmotic regulating substances to improve the drought resistance, cold resistance and salt-alkali resistance of plants, and can also promote protein synthesis and enhance the growth potential of plants.

[0016] Preferably, the preparation method of the modified carrier is as follows:

[0017] S1, adding sepiolite into an acidic potassium permanganate solution for immersion treatment, filtering, washing and drying after the treatment to obtain pretreated sepiolite;

[0018] S2, adding the pretreated sepiolite in step S1 into deionized water, then adding glutaraldehyde, chitosan, and glacial acetic acid, stirring to react, filtering, washing, and drying after the reaction is completed to obtain a composite sepiolite;

[0019] S3, adding the composite sepiolite in step S2 into DMF, then adding glutathione and indolebutyric acid, stirring evenly, adding EDC and NHS, and reacting at a constant temperature, filtering, washing, and drying after the reaction is completed to obtain modified sepiolite;

[0020] S4, adding the modified sepiolite in step S3 into deionized water, and then adding sodium silicate, heating to react, filtering, washing and drying after the reaction is completed to obtain a modified carrier.

[0021] Preferably, the mass concentration of potassium perpotassium in the acidic potassium permanganate solution in step S1 is 3-4%, and the pH is 4-5; the temperature of the immersion treatment is 30-40° C., and the time is 1-2 h.

[0022] In the present invention, acidic potassium permanganate is used to pretreat sepiolite, which can, on the one hand, clear the pore structure inside the sepiolite and increase its specific surface area and adsorption capacity, and on the other hand, activate the groups on its surface, which is beneficial to subsequent reactions.

[0023] Preferably, in step S2, the mass ratio of the pretreated sepiolite, glutaraldehyde, chitosan and glacial acetic acid is 40-50:10-15:15-20:5-8, the stirring reaction temperature is 50-60° C., and the time is 4-5 h.

[0024] In the present invention, glutaraldehyde is used as a crosslinking agent to introduce chitosan into sepiolite. On the one hand, chitosan has a large number of amino groups, which is beneficial to the subsequent reaction; on the other hand, chitosan has a good adsorption effect, which is beneficial to the subsequent adsorption of silicon.

[0025] Preferably, in step S3, the mass ratio of the composite sepiolite, glutathione, indolebutyric acid, EDC and NHS is 40-50:6-8:4-7:15-20:20-25; the temperature of the isothermal reaction is 70-80° C., and the time is 3-4 hours.

[0026] In the present invention, glutathione and indolebutyric acid are introduced into the composite sepiolite in the form of amide bonds through chemical reactions, so that the combination of glutathione, indolebutyric acid and the composite sepiolite is more stable. In the subsequent plant growth process, due to the hydrolysis of the amide bonds, glutathione and indolebutyric acid are slowly released, reducing the loss of the two in water and soil and improving their bioavailability; indolebutyric acid can promote the growth of plant roots, and a more developed root system can absorb more water and nutrients, making it easier for plants to survive under adverse conditions such as drought and barrenness; glutathione can remove active oxygen free radicals in plants and reduce the damage to plants caused by oxidative stress. The two can work together to improve the resistance of plants to adverse conditions such as salinity, drought, and heavy metals.

[0027] Preferably, in step S4, the mass ratio of the modified sepiolite to sodium silicate is 40-50:14-18, the temperature of the heating reaction is 60-70° C., and the time is 3-4 hours.

[0028] In the present invention, silicon is introduced into the modified sepiolite by adding sodium silicate, and silicon can strengthen the plant cell wall, reduce the plant's absorption of salt, further improve the plant's resistance to saline-alkali conditions, and also improve the plant's resistance to diseases and insect pests.

[0029] The present invention also protects a method for preparing the above-mentioned plant multi-effect stress resistance agent, comprising the following steps:

[0030] The raw materials are weighed according to the formula, and the modified carrier, zeolite powder, wood ash, compound amino acid, seaweed extract, humic acid, betaine, rhamnolipid and trace elements are evenly mixed to obtain the product.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] (1) The plant multi-effect stress-resistant agent provided by the present invention comprises a modified carrier, zeolite powder, plant ash, composite amino acid, seaweed extract, humic acid, betaine, rhamnolipid, and trace elements, which are compounded in a specific ratio. Zeolite powder can improve soil permeability, increase soil cation exchange capacity, adsorb harmful substances, and provide nutrition for plants; plant ash can provide mineral elements such as potassium and phosphorus, and regulate soil pH; composite amino acid can be used as a plant osmotic regulating substance to improve the plant's drought resistance, cold resistance, and salt-alkali resistance, and can also promote protein synthesis and enhance plant growth potential; seaweed extract The raw materials are rich in active substances such as plant growth hormones and cytokinins, which can promote plant growth, improve photosynthetic efficiency and enhance stress resistance; humic acid can improve soil structure, enhance water and fertilizer retention capacity, stimulate plant growth and improve root vitality; betaine, as an osmotic regulating substance, can improve plant drought and salt-alkali resistance, and can also protect cell membrane structure and reduce the damage to plants caused by adverse stress; rhamnolipid, as a surfactant, can promote plant absorption of nutrients and enhance plant resistance to pathogens; the synergistic effect of various raw materials can jointly improve the plant's stress resistance and growth and development level.

[0033] (2) The plant multi-effect stress resistance agent provided by the present invention has a modified carrier added, with sepiolite as the base material, which has a large specific surface area and adsorption capacity, can improve the soil structure and enhance the water and fertilizer retention capacity; the sepiolite is pretreated by acidic potassium permanganate to further improve its specific surface area and adsorption capacity; then chitosan is introduced into the sepiolite by using glutaraldehyde as a cross-linking agent. On the one hand, chitosan has a large number of amino groups, which is conducive to the subsequent reaction; on the other hand, chitosan has a good adsorption effect, which is conducive to the subsequent adsorption of silicon; then glutathione and indolebutyric acid are introduced into the composite sepiolite in the form of amide bonds through chemical reactions, so that the combination between glutathione, indolebutyric acid and the composite sepiolite is more stable. In the subsequent plant growth process, due to the hydrolysis of the amide bonds, glutathione and indolebutyric acid are slowly released. The indolebutyric acid can promote the growth of plant roots, and a more developed root system can absorb more water and nutrients, making it easier for plants to survive in adverse conditions such as drought and barrenness. Glutathione can remove active oxygen free radicals in plants and reduce the damage to plants caused by oxidative stress. The two can work together to improve the resistance of plants to adversities such as salinity, drought, and heavy metals. Finally, by adding sodium silicate, silicon is introduced into the modified sepiolite. Silicon can strengthen plant cell walls, reduce the absorption of salt by plants, and further improve the resistance of plants to salinity and alkali, as well as the resistance of plants to diseases and insect pests.

[0034] (3) The plant multi-effect stress-resistant agent provided by the present invention has added trace elements that can supplement the trace elements required for plant growth, promote enzyme activity, enhance plant metabolism, and thus improve the stress resistance and growth and development level of the plant; the added complex amino acids are composed of glycine, proline and γ-aminobutyric acid, which can be used as plant osmotic regulating substances to improve the plant's drought resistance, cold resistance and salt-alkali resistance, and can also promote protein synthesis and enhance the growth potential of the plant. DETAILED DESCRIPTION

[0035] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] In the present invention, the particle size of the zeolite powder is 200 meshes; the particle size of the sepiolite is 325 meshes.

[0037] Example 1

[0038] A plant multi-effect stress-resistant agent, comprising the following raw materials by weight:

[0039] 35 parts of modified carrier, 25 parts of zeolite powder, 8 parts of plant ash, 6 parts of complex amino acid, 8 parts of seaweed extract, 20 parts of humic acid, 4 parts of betaine, 2.5 parts of rhamnolipid and 0.7 parts of trace elements.

[0040] The trace elements are composed of iron sulfate, zinc sulfate, manganese sulfate and ammonium molybdate in a mass ratio of 1:0.5:0.4:0.2; the composite amino acid is a mixture of glycine, proline and γ-aminobutyric acid in a mass ratio of 5.5:2.5:2.5;

[0041] The preparation method of the modified carrier is as follows:

[0042] S1. Add 100 g of sepiolite into 800 mL of an acidic potassium permanganate aqueous solution (the mass concentration of potassium permanganate is 3%, and the pH is 4), and immerse the mixture at 35° C. for 1.5 h. After the treatment, filter, wash, and dry the mixture to obtain a pretreated sepiolite.

[0043] S2, adding 45g of the pretreated sepiolite in step S1 to 900g of deionized water, followed by adding 13g of glutaraldehyde, 18g of chitosan, and 7g of glacial acetic acid, stirring and reacting at 55°C for 4.5h, filtering, washing, and drying after the reaction is completed to obtain a composite sepiolite;

[0044] S3, adding 45g of the composite sepiolite in step S2 to 700mL of DMF, then adding 7g of glutathione and 6g of indolebutyric acid, stirring evenly, adding 18g of EDC and 23g of NHS, and reacting at a constant temperature of 75°C for 3.5h. After the reaction is completed, filtering, washing, and drying to obtain modified sepiolite;

[0045] S4, adding 45g of the modified sepiolite in step S3 into 800g of deionized water, and then adding 16g of sodium silicate, heating to react at 65°C for 3.5h, filtering, washing and drying after the reaction is completed to obtain a modified carrier.

[0046] A method for preparing a plant multi-effect stress-resistant agent comprises the following steps:

[0047] The raw materials are weighed according to the formula, and the modified carrier, zeolite powder, wood ash, compound amino acid, seaweed extract, humic acid, betaine, rhamnolipid and trace elements are evenly mixed to obtain the product.

[0048] Example 2

[0049] A plant multi-effect stress-resistant agent, comprising the following raw materials by weight:

[0050] 35 parts of modified carrier, 255 parts of zeolite powder, 7 parts of plant ash, 6 parts of complex amino acid, 7 parts of seaweed extract, 20 parts of humic acid, 4 parts of betaine, 2 parts of rhamnolipid, and 0.6 parts of trace elements.

[0051] The trace elements are composed of iron sulfate, zinc sulfate, manganese sulfate and ammonium molybdate in a mass ratio of 1: 0.6: 0.3: 0.2; the composite amino acid is a mixture of glycine, proline and γ-aminobutyric acid in a mass ratio of 5: 3: 2;

[0052] The preparation method of the modified carrier is as follows:

[0053] S1. Add 100 g of sepiolite into 800 mL of an acidic potassium permanganate aqueous solution (the mass concentration of potassium permanganate is 4%, and the pH is 5), and immerse the mixture at 35° C. for 1.5 h. After the treatment, filter, wash, and dry the mixture to obtain the pretreated sepiolite.

[0054] S2, adding 45g of the pretreated sepiolite in step S1 to 900g of deionized water, followed by adding 12g of glutaraldehyde, 17g of chitosan, and 6g of glacial acetic acid, stirring and reacting at 55°C for 4.5h, filtering, washing, and drying after the reaction is completed to obtain a composite sepiolite;

[0055] S3, adding 45g of the composite sepiolite in step S2 to 700mL of DMF, then adding 7g of glutathione and 5g of indolebutyric acid, stirring evenly, adding 17g of EDC and 22g of NHS, and reacting at a constant temperature of 75°C for 3.5h. After the reaction is completed, filtering, washing, and drying to obtain modified sepiolite;

[0056] S4, adding 45g of the modified sepiolite in step S3 into 800g of deionized water, and then adding 15g of sodium silicate, heating to react at 65°C for 3.5h, filtering, washing and drying after the reaction is completed to obtain a modified carrier.

[0057] A method for preparing a plant multi-effect stress-resistant agent comprises the following steps:

[0058] The raw materials are weighed according to the formula, and the modified carrier, zeolite powder, wood ash, compound amino acid, seaweed extract, humic acid, betaine, rhamnolipid and trace elements are evenly mixed to obtain the product.

[0059] Example 3

[0060] A plant multi-effect stress-resistant agent, comprising the following raw materials by weight:

[0061] 30 parts of modified carrier, 20 parts of zeolite powder, 5 parts of plant ash, 5 parts of complex amino acid, 5 parts of seaweed extract, 15 parts of humic acid, 3 parts of betaine, 2 parts of rhamnolipid, and 0.5 parts of trace elements.

[0062] The trace elements are composed of iron sulfate, zinc sulfate, manganese sulfate and ammonium molybdate in a mass ratio of 1:0.5:0.3:0.2; the composite amino acid is composed of glycine, proline and γ-aminobutyric acid in a mass ratio of 5:2:3;

[0063] The preparation method of the modified carrier is as follows:

[0064] S1. Add 100 g of sepiolite into 800 mL of an acidic potassium permanganate aqueous solution (the mass concentration of potassium permanganate is 3%, and the pH is 4), and immerse the mixture at 30° C. for 2 h. After the treatment, filter, wash, and dry the mixture to obtain the pretreated sepiolite.

[0065] S2, adding 40g of the pretreated sepiolite in step S1 to 900g of deionized water, followed by adding 10g of glutaraldehyde, 15g of chitosan, and 5g of glacial acetic acid, stirring and reacting at 50°C for 5h, filtering, washing, and drying after the reaction is completed to obtain a composite sepiolite;

[0066] S3, adding 40g of the composite sepiolite in step S2 to 700mL of DMF, then adding 6g of glutathione and 4g of indolebutyric acid, stirring evenly, adding 15g of EDC and 20g of NHS, and reacting at a constant temperature of 70°C for 4h. After the reaction is completed, filtering, washing, and drying to obtain modified sepiolite;

[0067] S4, adding 40g of the modified sepiolite in step S3 into 800g of deionized water, and then adding 14g of sodium silicate, heating and reacting at 60°C for 4h, filtering, washing and drying after the reaction is completed to obtain a modified carrier.

[0068] A method for preparing a plant multi-effect stress-resistant agent comprises the following steps:

[0069] The raw materials are weighed according to the formula, and the modified carrier, zeolite powder, wood ash, compound amino acid, seaweed extract, humic acid, betaine, rhamnolipid and trace elements are evenly mixed to obtain the product.

[0070] Example 4

[0071] A plant multi-effect stress-resistant agent, comprising the following raw materials by weight:

[0072] 40 parts of modified carrier, 30 parts of zeolite powder, 10 parts of plant ash, 7 parts of complex amino acid, 10 parts of seaweed extract, 25 parts of humic acid, 5 parts of betaine, 3 parts of rhamnolipid and 0.8 parts of trace elements.

[0073] The trace elements are composed of iron sulfate, zinc sulfate, manganese sulfate and ammonium molybdate in a mass ratio of 1:0.6:0.4:0.3; the composite amino acid is a mixture of glycine, proline and γ-aminobutyric acid in a mass ratio of 6:3:2;

[0074] The preparation method of the modified carrier is as follows:

[0075] S1. Add 100 g of sepiolite into 800 mL of an acidic potassium permanganate aqueous solution (the mass concentration of potassium permanganate is 4%, and the pH is 4), and immerse the mixture at 40° C. for 1 h. After the treatment, filter, wash, and dry the mixture to obtain a pretreated sepiolite.

[0076] S2, adding 50g of the pretreated sepiolite in step S1 to 900g of deionized water, followed by adding 15g of glutaraldehyde, 20g of chitosan, and 8g of glacial acetic acid, stirring and reacting at 60°C for 4h, filtering, washing, and drying after the reaction is completed to obtain a composite sepiolite;

[0077] S3, adding 50g of the composite sepiolite in step S2 to 700mL of DMF, then adding 8g of glutathione and 7g of indolebutyric acid, stirring evenly, adding 20g of EDC and 25g of NHS, and reacting at a constant temperature of 80°C for 3h. After the reaction is completed, filtering, washing, and drying to obtain modified sepiolite;

[0078] S4, adding 50g of the modified sepiolite in step S3 into 800g of deionized water, and then adding 18g of sodium silicate, heating and reacting at 70°C for 3h, filtering, washing and drying after the reaction is completed to obtain a modified carrier.

[0079] A method for preparing a plant multi-effect stress-resistant agent comprises the following steps:

[0080] The raw materials are weighed according to the formula, and the modified carrier, zeolite powder, wood ash, compound amino acid, seaweed extract, humic acid, betaine, rhamnolipid and trace elements are evenly mixed to obtain the product.

[0081] Comparative Example 1

[0082] A plant multi-effect stress-resistant agent, comprising the following raw materials by weight:

[0083] 35 parts of modified carrier, 25 parts of zeolite powder, 8 parts of plant ash, 6 parts of complex amino acid, 8 parts of seaweed extract, 20 parts of humic acid, 4 parts of betaine, 2.5 parts of rhamnolipid and 0.7 parts of trace elements.

[0084] The trace elements are composed of iron sulfate, zinc sulfate, manganese sulfate and ammonium molybdate in a mass ratio of 1:0.5:0.4:0.2; the composite amino acid is a mixture of glycine, proline and γ-aminobutyric acid in a mass ratio of 5.5:2.5:2.5;

[0085] The preparation method of the modified carrier is as follows:

[0086] S1. Add 100 g of sepiolite into 800 mL of an acidic potassium permanganate aqueous solution (the mass concentration of potassium permanganate is 3%, and the pH is 4), and immerse the mixture at 35° C. for 1.5 h. After the treatment, filter, wash, and dry the mixture to obtain a pretreated sepiolite.

[0087] S2, adding 45g of the pretreated sepiolite in step S1 to 900g of deionized water, followed by adding 13g of glutaraldehyde, 18g of chitosan, and 7g of glacial acetic acid, stirring and reacting at 55°C for 4.5h, filtering, washing, and drying after the reaction is completed to obtain a composite sepiolite;

[0088] S3, adding 45g of the composite sepiolite in step S2 into 800g of deionized water, and then adding 16g of sodium silicate, heating to react at 65°C for 3.5h, filtering, washing and drying after the reaction is completed to obtain a modified carrier.

[0089] A method for preparing a plant multi-effect stress-resistant agent comprises the following steps:

[0090] The raw materials are weighed according to the formula, and the modified carrier, zeolite powder, wood ash, compound amino acid, seaweed extract, humic acid, betaine, rhamnolipid and trace elements are evenly mixed to obtain the product.

[0091] Compared with the examples, glutathione and indolebutyric acid were not introduced into the modified carrier in this comparative example.

[0092] Comparative Example 2

[0093] A plant multi-effect stress-resistant agent, comprising the following raw materials by weight:

[0094] 35 parts of modified carrier, 25 parts of zeolite powder, 8 parts of plant ash, 6 parts of complex amino acid, 8 parts of seaweed extract, 20 parts of humic acid, 4 parts of betaine, 2.5 parts of rhamnolipid and 0.7 parts of trace elements.

[0095] The trace elements are composed of iron sulfate, zinc sulfate, manganese sulfate and ammonium molybdate in a mass ratio of 1:0.5:0.4:0.2; the composite amino acid is a mixture of glycine, proline and γ-aminobutyric acid in a mass ratio of 5.5:2.5:2.5;

[0096] The preparation method of the modified carrier is as follows:

[0097] S1. Add 100 g of sepiolite into 800 mL of an acidic potassium permanganate aqueous solution (the mass concentration of potassium permanganate is 3%, and the pH is 4), and immerse the mixture at 35° C. for 1.5 h. After the treatment, filter, wash, and dry the mixture to obtain a pretreated sepiolite.

[0098] S2, adding 45g of the pretreated sepiolite in step S1 to 900g of deionized water, followed by adding 13g of glutaraldehyde, 18g of chitosan, and 7g of glacial acetic acid, stirring and reacting at 55°C for 4.5h, filtering, washing, and drying after the reaction is completed to obtain a composite sepiolite;

[0099] S3, adding 45g of the composite sepiolite in step S2 into 700mL of DMF, followed by adding 7g of glutathione and 6g of indolebutyric acid, stirring evenly, adding 18g of EDC and 23g of NHS, and reacting at a constant temperature of 75°C for 3.5h. After the reaction is completed, filtering, washing and drying are performed to obtain a modified carrier.

[0100] A method for preparing a plant multi-effect stress-resistant agent comprises the following steps:

[0101] Weigh the raw materials according to the formula, and mix the modified carrier, zeolite powder, plant ash, compound amino acids, seaweed extract, humic acid, betaine, rhamnolipid, and trace elements evenly to obtain the product.

[0102] Compared with Example 1, sodium silicate was not introduced into the modified carrier in this comparative example.

[0103] Comparative Example 3

[0104] A plant multi-effect stress resistance agent, by weight, comprises the following raw materials:

[0105] 35 parts of modified carrier, 25 parts of zeolite powder, 8 parts of plant ash, 6 parts of compound amino acids, 8 parts of seaweed extract, 20 parts of humic acid, 4 parts of betaine, 2.5 parts of rhamnolipid, and 0.7 part of trace elements.

[0106] Among them, the trace elements are composed of ferric sulfate, zinc sulfate, manganese sulfate, and ammonium molybdate with a mass ratio of 1:0.5:0.4:0.2; the compound amino acids are mixed by glycine, proline, and γ-aminobutyric acid with a mass ratio of 5.5:2.5:2.5;

[0107] The preparation method of the modified carrier is as follows:

[0108] S1. Add 100 g of sepiolite to 800 mL of acidic potassium permanganate aqueous solution (the mass concentration of potassium permanganate is 3% and the pH is 4), impregnate at 35 °C for 1.5 h, filter, wash, and dry after the treatment to obtain pretreated sepiolite;

[0109] S2. Add 45 g of the pretreated sepiolite in step S1 to 900 g of deionized water, then add 13 g of glutaraldehyde, 18 g of chitosan, and 7 g of glacial acetic acid, stir and react at 55 °C for 4.5 h, filter, wash, and dry after the reaction to obtain composite sepiolite;

[0110] S3. Add 45 g of the composite sepiolite in step S2 to 700 mL of DMF, then add 7 g of glutathione and 6 g of indolebutyric acid, stir evenly, add 16 g of sodium silicate, stir at 65 °C for 3.5 h, filter, wash, and dry after stirring to obtain the modified carrier.

[0111] A preparation method of a plant multi-effect stress resistance agent, comprising the following steps:

[0112] Weigh the raw materials according to the formula, and mix the modified carrier, zeolite powder, plant ash, compound amino acids, seaweed extract, humic acid, betaine, rhamnolipid, and trace elements evenly to obtain the product.

[0113] Compared with Example 1, in this comparative example, glutathione, indolebutyric acid, and sodium silicate were introduced into sepiolite by physical mixing.

[0114] The effects of the plant multi-effect stress resistance agents prepared in Examples 1-4 and Comparative Examples 1-3 were verified as follows:

[0115] The saline-alkali soil was used as the material, the soil pH was 8.8, and the alkalinity was 17.6%; the potato crop was tested, and the variety was Longshu No. 7. The experiment set up a normal control group, a blank control group, an embodiment 1-4 group, and a comparative example 1-3 group, wherein embodiments 1-4 were respectively the plant multi-effect stress resisters prepared by embodiments 1-4, and comparative examples 1-3 were respectively the plant multi-effect stress resisters prepared by comparative examples 1-3, and the multi-effect stress resisters were diluted 1:500 times, and the dosage was 2 mL per kg of soil. The experiment was carried out in a potted plant. During the experiment, except for the normal control group, the other groups were subjected to moderate drought simulation (controlling the amount of water applied to about 45% of the normal required amount to simulate natural drought stress) and low temperature simulation (the ambient temperature of the artificial box was 10°C during the day and 4°C at night), the normal control group was greenhouse culture conditions, the soil was normal soil, the pH was 6.9, and normal water supply was given; the blank control group was the above-mentioned simulated drought and low temperature environmental conditions, and no plant multi-effect stress resister treatment was given; the test period was 60 days.

[0116] Electrolyte permeability (EL): Collect fresh leaves from the middle part, wrap them in absorbent paper and immerse them completely in distilled water. After soaking at 20℃ for 24h, use a conductivity meter to measure the initial conductivity value C 0 ; Then boil the leaves for 20 minutes and cool completely before measuring the final conductivity, recorded as C 1 , EL=C 0 / C 1 *100%; 20 leaves were measured in each group and the average value was taken.

[0117] Chlorophyll content (Chl): The conventional chlorophyll content determination method in the art can be adopted, for example: take 100 mg of lawn leaves and place them in a mortar, add a small amount of quartz sand, calcium carbonate and 80% acetone to fully grind and extract, centrifuge all the homogenates, discard the precipitate, take the supernatant and transfer it to a 20 ml volumetric flask, and dilute to the scale line with 80% acetone; use 80% acetone as a control, use an ultraviolet spectrophotometer to measure the absorbance values ​​at wavelengths of 663nm and 645nm, respectively, and then convert them into total chlorophyll content based on dry weight (mg / g DW). Each group is measured 3 times and the average value is taken. The results are shown in Table 1 below.

[0118] Table 1 Effects of different groups of plant multi-effect stress resistance agents on potato physiological parameters

[0119]

[0120] The electrolyte permeability represents the permeability of the plasma membrane, and also represents the degree and stability of damage. As can be seen from Table 1 above, the electrolyte permeability of potatoes is significantly increased and the chlorophyll content is significantly reduced after a period of drought and low temperature stress. The plant multi-effect stress resistance agent prepared by the present invention can significantly slow down the change trend and reduce the mortality rate of plants. The effect is better than that of the comparative examples 1-3. This shows that the multi-effect stress resistance agent prepared by the present invention can reduce the oxidative damage of drought and low temperature stress to cell membrane and chlorophyll, and promote the enhancement of stress resistance.

[0121] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A plant multi-effect stress resistance agent, characterized in that: By weight, it includes the following raw materials: 30-40 parts of modified carrier, 20-30 parts of zeolite powder, 5-10 parts of plant ash, 5-7 parts of complex amino acids, 5-10 parts of seaweed extract, 15-25 parts of humic acid, 3-5 parts of betaine, 2-3 parts of rhamnolipid, 0.5-0.8 parts of trace elements; Wherein, the preparation method of the modified carrier is as follows: S1, adding sepiolite into an acidic potassium permanganate solution for immersion treatment, filtering, washing and drying after the treatment to obtain pretreated sepiolite; S2, adding the pretreated sepiolite in step S1 into deionized water, then adding glutaraldehyde, chitosan, and glacial acetic acid, stirring to react, filtering, washing, and drying after the reaction is completed to obtain a composite sepiolite; S3, adding the composite sepiolite in step S2 into DMF, then adding glutathione and indolebutyric acid, stirring evenly, adding EDC and NHS, and reacting at a constant temperature, filtering, washing, and drying after the reaction is completed to obtain modified sepiolite; S4, adding the modified sepiolite in step S3 into deionized water, then adding sodium silicate, heating to react, filtering, washing and drying after the reaction is completed to obtain a modified carrier; The mass concentration of potassium perpotassium in the acidic potassium permanganate solution in step S1 is 3-4%, and the pH is 4-5; the temperature of the immersion treatment is 30-40°C, and the time is 1-2h; the mass ratio of the pretreated sepiolite, glutaraldehyde, chitosan, and glacial acetic acid in step S2 is 40-50:10-15:15-20:5-8, and the temperature of the stirring reaction is 50-60°C, and the time is 4-5h; the mass ratio of the composite sepiolite, glutathione, indolebutyric acid, EDC, and NHS in step S3 is 40-50:6-8:4-7:15-20:20-25; the temperature of the isothermal reaction is 70-80°C, and the time is 3-4h; the mass ratio of the modified sepiolite and sodium silicate in step S4 is 40-50:14-18, and the temperature of the heating reaction is 60-70°C, and the time is 3-4h.

2. The plant multi-effect stress resistance agent according to claim 1, characterized in that: The trace elements are composed of iron sulfate, zinc sulfate, manganese sulfate and ammonium molybdate in a mass ratio of 1:0.5-0.6:0.3-0.4:0.2-0.

3.

3. The plant multi-effect stress resistance agent according to claim 1, characterized in that: The composite amino acid is prepared by mixing glycine, proline and gamma-aminobutyric acid in a mass ratio of 5-6:2-3:2-3.

4. A method for preparing the plant multi-effect stress resistance agent according to any one of claims 1 to 3, characterized in that: The following steps are involved: The raw materials are weighed according to the formula, and the modified carrier, zeolite powder, wood ash, compound amino acid, seaweed extract, humic acid, betaine, rhamnolipid and trace elements are evenly mixed to obtain the product.

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