Glutamate decarboxylase mutant and application thereof

CN119799683AActive Publication Date: 2025-04-11ANHUI BBCA FERMENTATION TECH ENG RES
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Patent Information

Application Number
CN202411813134.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-05
Publication Date
2025-04-11
Estimated Expiration
2044-12-05

AI Technical Summary

Technical Problem

The existing glutamate decarboxylases are prone to inactivation at higher pH values, limiting the industrial production of γ-aminobutyric acid.

Method used

A glutamate decarboxylase mutant was developed to improve the stability and activity of the enzyme within a wide pH range by introducing specific mutations (T62S, D68H, E218K and I366S) in amino acids 62, 68, 218 and 366.

Benefits of technology

It realizes efficient catalysis of glutamate decarboxylase in pH 3.8-7.7, reduces the use of acid pH adjustment, reduces material consumption, and avoids the formation of inorganic salts, simplifies the post-extraction process.

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Abstract

The invention discloses a glutamic acid decarboxylase mutant, relative to an amino acid sequence of wild type glutamic acid decarboxylase, the glutamic acid decarboxylase mutant has mutation at one or more of 62nd, 68th, 218th and 366th amino acids, and the wild type glutamic acid decarboxylase is glutamic acid decarboxylase derived from Escherichia coli. The glutamic acid decarboxylase mutant provided by the invention is used for catalyzing glutamic acid in a wide pH value range to prepare gamma-aminobutyric acid, and the pH value does not need to be adjusted by using an acid in a catalytic reaction, so that the material consumption is reduced, the generation of inorganic salt is avoided, and the post-extraction process is simplified.
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Citation Information

Patent Citations

  • Glutamic acid decarboxylase mutant and application thereof

    CN111944795A

  • Glutamate decarboxylase and gamma-aminobutyric acid high-yield strain

    CN112831488A

  • Glutamate decarboxylase mutant and application thereof

    CN112899260A

  • Industrial fermentation production method of whole cells containing glutamate decarboxylase

    CN113249364A

  • Glutamate decarboxylase mutant and use in production of γ-aminobutyric acid

    WO2023240871A1

Cited By

  • Sulfoalanine decarboxylase mutant with improved enzyme activity and application thereof in production of taurine

    CN121780497A