Observation device and observation method for puncture specimen

Abstract

The observation device comprises an electronic balance, the electronic balance comprises a base, a weighing element is arranged in the base, a weighing tray is arranged on the weighing element, the weighing tray is covered with a windproof cover, an operation panel is arranged on the surface of the base, adjustable supporting legs are arranged on one side of a chassis, and the adjustable supporting legs are arranged on the other side of the chassis. An optical magnification observation device capable of being aligned with the weighing tray is arranged at the tail end of the adjustable supporting leg, the weighing tray comprises a bottom support, a light source is arranged on the bottom support, a transparent cover covers the light source, and scale marks are arranged on the surface of the transparent cover. The observation device can simultaneously measure and count the length and the weight of the core tissue of the puncture specimen at one time, the observation method quantitatively evaluates the quality of the puncture specimen through the length of the core tissue of the puncture specimen and the weight of a formed tissue strip, an operator is helped to judge the specimen in real time in an operation, the specimen quality is improved, and the working efficiency is improved. The number of puncture needles is reduced, and the situation of secondary biopsy of a patient is avoided.

Description

Technical Field

[0001] The invention belongs to the technical field of puncture specimen processing, and in particular relates to a puncture specimen observation device and an observation method. Background Art

[0002] Lung cancer is the leading cause of malignant tumor morbidity and mortality worldwide. Systematic and accurate TNM staging is the cornerstone for selecting treatment methods and predicting prognosis for lung cancer patients. Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is recommended by the National Comprehensive Cancer Network (NCCN) and the American College of Chest Physicians (ACCP) as the first-line invasive examination for mediastinal staging of lung cancer due to its advantages such as minimal invasiveness, high efficiency, few complications, and good repeatability. However, due to the sampling method of EBUS-TBNA "needle aspiration", histological specimens may have problems such as limited tissue volume, small and broken tissue, and compression, which poses a great challenge to pathological diagnosis. In addition, with the continuous advancement of molecular diagnostic technology, lung cancer treatment has entered the era of precision treatment. The selection of regimens and drugs is jointly formulated according to its histological subtype and molecular test results. In addition to routine pathological examination, specimens obtained by EBUS-TBNA need to undergo molecular typing and genetic testing, which places high demands on the quality of EBUS-TBNA small sample biopsy tissue. However, currently, there is no real-time, simple method for operators to evaluate the quality of puncture specimens during EBUS-TBNA. Operators usually judge the specimen quality and the number of puncture needles based on their operating experience, but this may increase the risk of re-biopsy and complications for patients. Summary of the invention

[0003] The technical problem to be solved by the present invention is to provide a device and method for observing a puncture specimen, which can quantitatively evaluate the puncture specimen from multiple aspects and angles.

[0004] The technical solution adopted by the present invention to solve its technical problem is: to provide an observation device for puncture specimens, including an electronic balance, the electronic balance including a base, a weighing element is arranged in the base, a weighing tray is arranged on the weighing element, a windproof cover is arranged on the outside of the weighing tray, an operation panel is arranged on the surface of the base, an adjustable leg is arranged on one side of the chassis, an optical magnification observation device which can be aligned with the weighing tray is arranged at the end of the adjustable leg, the weighing tray includes a bottom support, a light source is arranged on the bottom support, a transparent cover is covered on the light source, and scale lines are arranged on the surface of the transparent cover.

[0005] Preferably, the optical magnification observation device is a magnifying glass.

[0006] Preferably, the magnifying glass is an electronic magnifying glass, and the electronic magnifying glass is connected to an external display.

[0007] Preferably, leveling feet are provided at the four corners of the bottom of the base.

[0008] Preferably, the operation panel includes a display screen for indicating weight, a main switch, a tare key and a calibration key, and the operation panel also includes a light switch and a brightness adjustment knob for controlling the light source.

[0009] Preferably, the light source is an LED lamp, and the light emitted by the LED lamp irradiates the transparent cover upward.

[0010] Preferably, an operating door is provided on a side of the wind shield close to the operating panel.

[0011] In order to solve the above technical problems, the present invention further provides a method for observing a puncture specimen, using the above-mentioned puncture specimen observation device, comprising the following steps:

[0012] S1. Before EBUS-TBNA puncture for each lesion, prepare 2 culture dishes, each containing 2-3 mL of liquid-based cell preservation solution. Culture dish A is placed on the operating table, and culture dish B is placed on the transparent cover of the weighing tray and returned to zero using the tare key.

[0013] S2. After each puncture, insert the needle core of the EBUS-TBNA puncture needle into the needle track, and slowly inject the puncture specimen in the needle track into the culture dish A. After the needle core is withdrawn, use a syringe to draw air to spray the residual specimen in the needle track into the bottle of liquid-based cell preservation solution. If there are still tissue strips during the spraying process, use tweezers to put the tissue strips into the culture dish A as well.

[0014] S3, shaking culture dish A to dissolve the blood clot in the puncture specimen, washing the blood, using tweezers to move the formed tissue strip in the puncture specimen into culture dish B, and then obtaining the weight of the puncture specimen;

[0015] S4, turning on the light source, observing the presence of the core tissue in the tissue strip through the optical magnification observation device, and measuring and counting the cumulative length with the help of the scale lines at the bottom of the culture dish;

[0016] S5. Select different puncture specimen qualification standards according to the needle diameter of the puncture needle used, compare the core tissue length and weight of the puncture specimen with the puncture specimen qualification standards, and if they are not met, puncture again, and then calculate the cumulative puncture tissue weight and length until the specimen qualification standards are met.

[0017] Preferably, the core tissue includes tissue strips having white, flesh-colored, and red and white colors.

[0018] Preferably, when the EBUS-TBNA puncture needle is 22G, the qualification standard of the puncture specimen is: the core tissue length of the puncture specimen is ≥8.5mm, or the weight of the formed tissue strip is ≥65.5mg.

[0019] The beneficial effect is that the observation device of the present invention can measure and count the length and weight of the core tissue of the puncture specimen at one time, and the observation method and observation device of the present invention can be used to quantitatively evaluate the quality of the puncture specimen in terms of the length of the core tissue of the puncture specimen and the weight of the formed tissue strip, thereby helping the operator to judge the quality of the puncture specimen in real time during the EBUS-TBNA operation, improve the specimen quality, reduce the number of puncture needles, and avoid the occurrence of secondary biopsies for patients. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 A stereoscopic diagram of a device for observing puncture specimens.

[0021] Figure 2 for Figure 1 Enlarged exploded view of the weighing tray in the middle.

[0022] Among them, 1-base; 2-weighing tray; 201-bottom support; 202-light source; 203-transparent cover; 204-scale line; 3-windproof cover; 4-adjustable legs; 5-optical magnification observation device; 6-operation panel; 7-operation door.

[0023] The same reference numerals in the various drawings represent the same components. DETAILED DESCRIPTION

[0024] The present invention will be further described below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms fall within the scope limited by the appended claims of the application equally.

[0025] like Figure 1 As shown, the present invention provides an observation device for puncture specimens, including an electronic balance, the electronic balance including a base 1, a weighing element is arranged inside the base 1, a weighing tray 2 is arranged on the weighing element, the weighing tray 2 is covered with a windshield 3, an operation panel 6 is arranged on the surface of the base 1, an adjustable leg 4 is arranged on one side of the chassis, an optical magnifying observation device 5 which can be aligned with the weighing tray 2 is arranged at the end of the adjustable leg 4, the weighing tray 2 includes a bottom support 201, a light source 202 is arranged on the bottom support 201, a transparent cover 203 is covered on the light source 202, and scale lines 204 are arranged on the surface of the transparent cover 203.

[0026] The structure of the electronic balance is similar to that of commercially available precision electronic balances, and is mainly used to weigh the mass of objects. It can weigh the mass of tissue strips at mg level with high precision. The weighing element includes a weighing sensor, which is accurate and reliable in weighing, displays quickly and clearly, and has an automatic detection system, a simple automatic calibration device, and an overload protection device. The weighing sensor is connected to the operating panel 6, and the standard operating panel 6 includes a display screen for weight display, a main switch, a peeling key, and a calibration key. A level is also provided on the base 1, and leveling feet are provided at the four corners of the bottom of the base 1 to ensure that the electronic balance is in a horizontal state and reduce measurement errors.

[0027] The optical magnifying observation device 5 is a magnifying glass, which can be an ordinary magnifying glass or an electronic magnifying glass. The ordinary magnifying glass or the electronic magnifying glass are both commercially available existing devices. The optical magnifying observation device 5 can be placed on the upper part of the wind shield 3 through the adjustable legs 4, so that the operator can clearly observe the puncture specimen on the weighing tray 2 outside the wind shield 3 and judge the length of the core tissue in the puncture specimen through the scale line 204.

[0028] In a specific embodiment, the magnifying glass is an electronic magnifying glass, which uses an electronic camera containing an optical system to output the captured image to an external display. At the same time, the electronic magnifying glass adjusts its position relative to the wind shield 3 through the adjustable legs 4. The adjustable legs 4 include a plurality of connecting rods connected by a universal ball head and are arranged on one side of the base 1. One of the connecting rods arranged along the height direction includes a matching telescopic outer rod and a telescopic inner rod. The telescopic inner rod is arranged inside the telescopic outer rod and can be telescoped along the length direction of the telescopic outer rod and fixed by interference fit to adjust the height of the electronic magnifying glass to ensure that the operator can adjust the focal length as needed to align with the formed tissue strip.

[0029] In such Figure 2 In a specific embodiment shown, the light source 202 includes a plurality of LED lamps arranged in an array in the transparent cover, and the emitted light of the LED lamps irradiates the transparent cover 203 upward. The operation panel 6 is also provided with a light switch and a brightness adjustment knob for controlling the light source 202, so as to ensure that the internal puncture specimen can still be clearly seen under different external lighting conditions. The light switch is used to turn on and off the light source 202, and the brightness adjustment knob is used to adjust the brightness of the light source 202. The wavelength of the LED lamp is fixed to 605nm after being turned on. The light of 605nm wavelength effectively filters the influence caused by blood when observing the specimen. The light source 202 is electrically connected to the power supply, light switch and brightness adjustment knob provided in the base 1 through the hole provided on the base 201.

[0030] In a specific embodiment, an operating door 7 is provided on one side of the wind shield 3 close to the operating panel 6, and the operator can place the formed tissue strip into the culture dish through the operating door 7, or unfold the formed tissue strip using tools such as tweezers to cooperate with the optical magnification observation device 5 and the scale line 204 to determine the length of the formed tissue strip.

[0031] The present invention also provides a method for observing a puncture specimen, using the above-mentioned puncture specimen observation device, comprising the following steps:

[0032] S1. Before EBUS-TBNA puncture for each lesion, prepare 2 culture dishes, each containing 2-3 mL of liquid-based cell preservation solution. Culture dish A is placed on the operating table, and culture dish B is placed on the transparent cover of the weighing tray and returned to zero using the tare key.

[0033] S2. After each puncture, insert the needle core of the EBUS-TBNA puncture needle into the needle track, and slowly inject the puncture specimen in the needle track into the culture dish A. After the needle core is withdrawn, use a syringe to draw air to spray the residual specimen in the needle track into the bottle of liquid-based cell preservation solution. If there are still tissue strips during the spraying process, use tweezers to put the tissue strips into the culture dish A as well.

[0034] S3, shaking culture dish A to dissolve the blood clot in the puncture specimen, washing the blood, using tweezers to move the formed tissue strip in the puncture specimen into culture dish B, and then obtaining the weight of the puncture specimen;

[0035] S4, turning on the light source, observing the presence of the core tissue in the tissue strip through the optical magnification observation device, and measuring and counting the cumulative length with the help of the scale lines at the bottom of the culture dish;

[0036] S5. Select different puncture specimen qualification standards according to the needle diameter of the puncture needle used, compare the core tissue length and weight of the puncture specimen with the puncture specimen qualification standards, and if they are not met, puncture again, and then calculate the cumulative puncture tissue weight and length until the specimen qualification standards are met.

[0037] Among them, in S4, the core tissue includes tissue strips with white, flesh-colored and red and white colors.

[0038] In S5, in actual use, the most commonly used EBUS-TBNA puncture needle diameter is 22G, and the puncture specimen qualification standard is: the core tissue length of the puncture specimen is ≥8.5mm, or the weight of the formed tissue strip is ≥65.5mg.

[0039] In S5, if there is still not enough specimen after 4-5 repeated punctures, the affected area is judged to be unsuitable for puncture biopsy, and the biopsy method should be changed, such as electrosurgical assisted fenestration biopsy forceps biopsy under the guidance of ultrasound bronchoscope, transbronchial mediastinal cryobiopsy under real-time guidance of endobronchial ultrasound, and other techniques.

[0040] After the puncture specimen is judged to be qualified, the formed tissue strip in culture dish B is transferred to a pathology bottle containing formalin solution for histological pathology examination, and the solution in culture dish B and culture dish A is transferred together to a liquid-based cell preservation solution bottle for cytological pathology examination.

Claims

1. A biopsy specimen observation device, comprising an electronic balance, the electronic balance comprising a base, a weighing element disposed in the base, a weighing tray disposed on the weighing element, a windshield covering the weighing tray, an operation panel disposed on the surface of the base, characterized in that: An adjustable leg is provided on one side of the chassis, and an optical magnifying observation device which can be aligned with the weighing tray is provided at the end of the adjustable leg. The weighing tray includes a base, a light source is provided on the base, a transparent cover is covered on the light source, and scale lines are provided on the surface of the transparent cover.

2. The observation device for puncture specimens according to claim 1, characterized in that: The optical magnifying observation device is a magnifying glass.

3. The observation device for puncture specimens according to claim 2, characterized in that: The magnifying glass is an electronic magnifying glass, and the electronic magnifying glass is connected to an external display.

4. The observation device for puncture specimens according to claim 1, characterized in that: Leveling feet are arranged at four corners of the bottom of the base.

5. The observation device for puncture specimen according to claim 1, characterized in that: The operation panel includes a display screen for indicating weight, a main switch, a tare key and a calibration key. The operation panel also includes a light switch and a brightness adjustment knob for controlling the light source.

6. The observation device for puncture specimens according to claim 1, characterized in that: The light source is an LED lamp, and the light emitted by the LED lamp irradiates the transparent cover upward.

7. The observation device for puncture specimens according to claim 1, characterized in that: An operating door is arranged on one side of the wind shield close to the operating panel.

8. A method for observing a puncture specimen, characterized in that: The invention according to any one of claims 1 to 8 comprises the following steps: S1. Before EBUS-TBNA puncture for each lesion, prepare 2 culture dishes, each containing 2-3 mL of liquid-based cell preservation solution. Culture dish A is placed on the operating table, and culture dish B is placed on the transparent cover of the weighing tray and returned to zero using the tare key. S2. After each puncture, insert the needle core of the EBUS-TBNA puncture needle into the needle track, and slowly inject the puncture specimen in the needle track into the culture dish A. After the needle core is withdrawn, use a syringe to draw air to spray the residual specimen in the needle track into the bottle of liquid-based cell preservation solution. If there are still tissue strips during the spraying process, use tweezers to put the tissue strips into the culture dish A as well. S3, shaking culture dish A to dissolve the blood clot in the puncture specimen, washing the blood, using tweezers to move the formed tissue strip in the puncture specimen into culture dish B, and then obtaining the weight of the puncture specimen; S4, turning on the light source, observing the presence of the core tissue in the tissue strip through the optical magnification observation device, and measuring and counting the cumulative length with the help of the scale lines at the bottom of the culture dish; S5. Select different puncture specimen qualification standards according to the needle diameter of the puncture needle used, compare the core tissue length and weight of the puncture specimen with the puncture specimen qualification standards, and if they are not met, puncture again, and then calculate the cumulative puncture tissue weight and length until the specimen qualification standards are met.

9. The method for observing a puncture specimen according to claim 8, characterized in that: The core tissue includes tissue strips in white, flesh-colored, and red and white colors.

10. The method for observing a puncture specimen according to claim 8, characterized in that: When the EBUS-TBNA puncture needle is 22G, the qualification standard of the puncture specimen is: the core tissue length of the puncture specimen is ≥8.5mm, or the weight of the formed tissue strip is ≥65.5mg.

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