Screening and compounding method for preparing lasioderma serricorne food attractant by using spice monomer

Abstract

The invention discloses a screening and compounding method for preparing a lasioderma serricorne food attractant by using a spice monomer, which comprises the following steps: S1, primarily selecting M spice monomer standard substances from a tobacco spice monomer library, and primarily selecting the attractant by using a trapping plate selection device, s2, adopting a monomer with a relatively good attraction effect as a main component, diluting to a certain concentration, testing the attraction effect by using an insect olfactometer, determining the optimal attraction dosage, and compounding according to the principle that the rest are auxiliary components, screening 1-2 formulas with relatively good effects, and determining the compound dosage; and S3, performing porous starch embedding, agar gel inclusion, silica gel pad slow release and other dosage form treatments on the monomer or the formula, and analyzing the residual period by using gas chromatography to determine the optimal carrier dosage form. According to the food attractant, male insects and female insects are trapped, the lasioderma serricorne trapping efficiency is improved, target targeting of the spice monomer and the tobacco homologous odorant is the same, ingredients are definite, quantification is accurate, industrial production is facilitated, and data support is provided for tobacco insect monitoring.

Description

Technical Field

[0001] The invention relates to the technical field of tobacco leaf storage pest control, and in particular to a screening and compounding method for preparing tobacco beetle feeding attractants using flavoring monomers. Background Art

[0002] Food attractants are biological attractants that simulate the smell of pest food and are artificially synthesized or assembled. Tobacco flavor monomers are divided into natural and artificially synthesized ones. They play an important role in flavoring cigarettes and are important odorants that attract tobacco pests. They are homologous to the attractant factors of tobacco odorants.

[0003] Attractants can kill male and female adults, thereby reducing the base number of insect sources for the next generation. However, the trapping efficiency of a single attractant is currently low. Most attractants are extracted from plants or formed naturally, and the content of their ingredients is relatively complex. It is difficult to form a standard for them, and it is impossible to form a method to determine the ingredients and concentration. This method determines the name and measurement of the ingredients of the attractant through screening and compounding of tobacco flavor monomers, and measures its attracting effect through an olfactometer. Combined with the compounding with sex attractants, the trapping efficiency is greatly improved, providing data support for the monitoring and prevention of tobacco storage and production. The green ecology of attractants, and the "green, healthy, safe, environmentally friendly, and efficient" pest ecological control technology system are the inevitable trend of social development. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide a screening and compounding method for preparing tobacco beetle food attractants using spice monomers, by preliminarily selecting spice monomers with good attracting effects from a tobacco spice library, using a trapping plate method and an olfactometer to screen out spice monomers with good attracting effects, and after compounding attracting effect tests, screening out compound formulas with high attracting indexes, determining the carrier dosage form, and preparing food attractants. This method is different from sex attractants that mainly trap male insects. Food attractants trap both male and female insects, thereby improving the trapping efficiency of tobacco beetles. The food attractants prepared by spice monomers and tobacco homologous odorants have the same target targeting. At the same time, the spice monomers themselves are green and environmentally friendly food attractants, and the components are clear and quantifiable, which is convenient for industrial production and provides data support for tobacco pest monitoring.

[0005] The technical problem to be solved by the present invention is achieved through the following technical solutions:

[0006] A method for screening and compounding a tobacco beetle feeding attractant using a flavoring monomer comprises the following steps:

[0007] S1. Preliminarily select M flavor monomer standards from the tobacco flavor monomer library, M≥15, and use a trapping plate selection device to preliminarily select attractants;

[0008] S2. Use monomers with better attractant effects as the main ingredients, dilute them to a certain concentration, test the attractant effects with an insect olfactometer, determine the best attractant dosage, and compound the rest as auxiliary ingredients, select 1-2 formulas with better effects, and determine the compound dosage;

[0009] S3. The monomer or formulation is treated with a variety of carrier dosage forms, wherein the carrier dosage form is: porous starch embedding, agar gel encapsulation or silica gel pad sustained release, and the residual period is analyzed by gas chromatography to determine the best carrier dosage form.

[0010] Preferably, in the above technical solution, in step S1, the preliminarily selecting M kinds of fragrance monomer standards comprises:

[0011] M kinds of flavor monomers were initially screened out from the tobacco flavor library, M≥15. The flavor monomers included alcohols, lipids, ketones, ethers or hydrocarbon compounds. The 17 flavor monomers initially screened were: phenylethyl alcohol, methyl phenethyl ether, 3-phenylpropyl isobutyrate, 3-phenylpropyl butyrate, (9Z,12E)-9,12-tetracyclopentadiene acetate, 3-phenylpropyl acetate, cyclohexyl cinnamate, benzyl phenylacrylate, 3-phenylpropyl, phenylethyl acetate, dimethylbenzyl butyrate, amyl cinnamate, diphenylacetone, 3-phenylpropyl valerate, cis-3-vinyl cinnamate, megastigmatrienone and ethyl 2-methylbutyrate. The above flavor monomers were diluted to a certain concentration with n-hexane.

[0012] Preferably, in the above technical solution, in step S1, the cultivation of the test tobacco beetle for primary selection of attractants comprises:

[0013] Feed: 90% whole wheat flour mixed with 10% yeast powder; Feeding conditions: temperature (28±2)℃, relative humidity (70±5)%;

[0014] Rearing method: Add 1 / 3 volume of feed to a clean and dried glass jar (volume 375mL), take an appropriate number of tobacco beetle adults with normal behavior and place them on the surface of the feed; dig a hole with a diameter of 5cm in the bottle cap of the rearing jar, line the inner side of the cap with qualitative filter paper with the same diameter as the lid and seal the bottle, place it in an artificial climate box with a temperature of (28±2)℃ and a relative humidity of (70±5)% for lightless cultivation; rotate the insects every 7d to ensure the consistency of the test insect generations; before the test, pick tobacco beetle adults that have emerged for 7-14d, put them into an empty glass bottle without feed and starve them for 24h for use.

[0015] Preferably, in the above technical solution, in step S1, the trapping method used for the initial selection of attractants specifically includes:

[0016] (1) Test method for trapping plate selection device:

[0017] At a temperature of 28±2℃ and a relative humidity of 70±5%, 100 tobacco beetle adults were placed in a sealed container of a fixed size, and the attracting effect of the flavor monomer was tested in a simulated experimental chamber, the number of adults on the trapping board was measured for 24 hours, and a control test was set up to determine the attracting effect; a 25mm long conventional cigarette filter stick was glued to the trapping board, 1-2ml of tobacco flavor monomer was sucked with a quantitative straw and dripped on the filter stick, and then hung in a simulated experimental chamber, and water was dripped on the filter stick of a blank trapping board as a control, and the distribution of the test insects on the trapping board was observed after 24 hours of exposure, and the test was repeated 3 times;

[0018] (2) Comparative calculation of the trend effect of tobacco beetle adults. The specific calculation method and data analysis are as follows:

[0019] Attraction rate (%) = (number of insects attracted by treatment - number of insects in control) * 100 / total number of insects tested.

[0020] Preferably, in the above technical solution, in step S2, the attracting effect is tested using an insect olfactometer, the insect olfactometer is a "Y" type insect olfactometer, and the attracting index of the test includes:

[0021] (1) The two arms of the "Y"-shaped insect olfactometer are connected to two pear-shaped bottles respectively. The pear-shaped bottles contain tobacco flavor monomers. Before the airflow enters the pear-shaped bottles, it is first filtered with activated carbon to purify the air, and the air is humidified by a device that filters the air with water; the air flow rate is controlled between 100-120mL / min; the experiment needs to be carried out in the dark, and a fluorescent tube wrapped with red cloth is installed about 300mm above the olfactometer; during the experiment, one of the pear-shaped bottles of the two-arm olfactometer is set as the test odor source, and the other pear-shaped bottle is blank as a control; then release tobacco beetle adults from the center of the activity room, and observe and record the insect's behavioral choices at the set time points; the test insects that crawl within 100mm of the air inlet of the arm are regarded as test insects with behavioral choice responses, and the remaining individuals are regarded as test insects without behavioral choice responses. Each treatment is repeated 3 times;

[0022] (2) According to the calculation method of repellency coefficient, determine the calculation formula of attraction index:

[0023] Attraction rate EPI (%) = (number of insects attracted by treatment - number of insects in control) * 100 / total number of insects tested

[0024] AI (%) = [(TC) / (T+C)] × 100

[0025] In the formula: AI is the attraction index; T is the number of adult tobacco beetles in the treatment, heads; C is the number of adult tobacco beetles in the control, heads; if AI is a negative number, it means a repellent effect.

[0026] Five fragrance monomers with relatively high attraction rates were screened, namely: hexyl acetate (1#), 3-phenylpropyl acetate (2#), (9Z,12E)-9,12-tetracyclopentadiene acetate (3#), 3-phenylpropyl butyrate (4#), and 3-phenylpropyl isobutyrate (5#).

[0027] Preferably, in the above technical solution, in step S2, designing the optimal measurement includes:

[0028] Select standard products with better attractant effects and dilute them to certain concentrations, such as 0.1%, 1%, 10%, and 100%, to determine the best attractant dosage; based on the best dosage, compound the products with the best attractant effect as the main component and the others as auxiliary components, screen a formula with better effects, and clarify the compound dosage.

[0029] Preferably, in the above technical solution, in step S2, designing a compound formula includes:

[0030] Five fragrance monomers with better attractant effects were preliminarily selected through the trapping plate, and the standard samples with better attractant effects were selected and diluted to a certain concentration to determine the optimal attractant dose; according to the optimal dose, the monomer with the best attractant effect was used as the main and the others as auxiliary to compound, and two of them were selected for compounding and screening, and the mass ratio of the material components in the compound was 1:1, 1:1:1, 1:1:1:1 or 1:1:1:1:1; the insect olfactometer was used to test the attraction index of the compound formula, a formula with better effect was screened, and the compound dosage was clarified.

[0031] Preferably, in the above technical solution, in step S3, the determination of the optimal carrier dosage form includes:

[0032] The formula was embedded in porous starch, encapsulated in agar gel, and sustained-released on a silica pad. The residual period was analyzed by gas chromatography every 1, 3, and 5 days. Based on the residual period, the silica pad sustained-release was determined to be the best food attractant carrier dosage form.

[0033] Preferably, in the above technical solution, the residual effect period refers to the time required for the content of tobacco flavor monomers to be reduced by more than 75%. The residual time of attractants of different flavor monomer varieties is different, and the residual period of attractants is generally 2-7 days.

[0034] Preferably, in the above technical solution, evaluating the trapping effect includes:

[0035] The prepared attractants were tested in a simulated experimental warehouse. The attractant core was combined with a trapping plate, 50-100 adult storage pests were placed in a sealed container of fixed size, the number of adults on the trapping plate was measured for 24 hours, and a sex pheromone attractant core and its combined control test were set up to determine the attractant effect.

[0036] A method for preparing a food attractant for improving the trapping efficiency of tobacco beetles comprises the following steps:

[0037] (1) Mixing fragrance monomers in proportion, wherein the fragrance monomers are phenylethyl acetate, 3-phenylpropyl acetate, 3-phenylpropyl butyrate, 3-phenylpropyl isobutyrate, and (9Z,12E)-9,12-tetracyclopentadiene acetate, preferably phenylethyl acetate and 3-phenylpropyl acetate are mixed in a mass ratio of 1:1 or phenylethyl acetate and (9Z,12E)-9,12-tetracyclopentadiene acetate are mixed in a mass ratio of 1:1;

[0038] (2) Dilute the mixture to 12.5 nL / cm 2 The concentration was , and the diluted mixture was treated with a silica pad sustained release dosage form;

[0039] (3) The treated food attractant is used in combination with sex pheromone to obtain a food attractant that improves the trapping efficiency of tobacco beetles.

[0040] The above technical solution of the present invention has the following beneficial effects:

[0041] The screening and compounding method for preparing tobacco beetle food attractants using flavor monomers of the present invention screens tobacco flavor monomers with better tobacco beetle pest attracting effect, determines the carrier dosage form, prepares monomers or compound food attractants. The attractant has the same odor as the tobacco raw material, has clear target targeting and strong pertinence, and the ingredients of the prepared food attractant are clearly measured, which is convenient for industrial production and simple to operate. At the same time, the food attractant is green and ecological, providing data support for the "green, healthy, safe, environmentally friendly and efficient" pest ecological control technology. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate embodiments of the invention and, together with the description, serve to explain the principles of the invention.

[0043] Figure 1 Schematic diagram of the screening and compounding method for making tobacco beetle attractants using spice monomers. DETAILED DESCRIPTION

[0044] Various exemplary embodiments of the present invention will now be described in detail. It should be noted that the relative arrangement of components and steps, numerical expressions and numerical values ​​set forth in these embodiments do not limit the scope of the present invention unless otherwise specifically stated.

[0045] Example 1

[0046] 1 Materials and methods

[0047] Test insects:

[0048] The adult tobacco beetles used in the experiment were artificially raised in the tobacco storage laboratory of Zhengzhou Tobacco Research Institute.

[0049] Feed: 90% whole wheat flour mixed with 10% yeast powder;

[0050] Rearing conditions: temperature (28±2)℃, relative humidity (70±5)%.

[0051] Rearing method: Add about 1 / 3 of the volume of feed to a clean and dried glass jar (volume 375mL), and place an appropriate number of tobacco beetle adults with normal behavior on the surface of the feed. Dig a hole with a diameter of 5cm in the bottle cap of the breeding jar, line the inner side of the lid with qualitative filter paper of the same diameter as the lid, and seal the bottle. Place it in an artificial climate box with a temperature of (28±2)℃ and a relative humidity of (70±5)% for lightless cultivation. Rotate the insects every 7 days to ensure the consistency of the test insect generations. Before the test, pick tobacco beetle adults that have emerged for 7-14 days, put them in an empty glass bottle without feed, and starve them for 24 hours for use.

[0052] Tobacco monomer flavors: phenylethyl alcohol, methyl phenethyl ether, megastigmatrienone, 3-phenylpropyl isobutyrate, 3-phenylpropyl butyrate, 3-phenylpropyl hexanoate, cyclohexyl cinnamate, benzyl phenylacrylate, anisehole, hexyl acetate, phenethyl acetate, 3-phenylpropyl acetate, amyl cinnamate, diphenylacetophenone, 3-phenylpropyl valerate, 3-phenylpropyl cinnamate, cis-3-hexenol formate, nicotinone, isopentyl butyrate and menthol; diluted with n-hexane to a certain concentration.

[0053] Experimental instruments: trapping plate (Zhangzhou Yinggeer Agricultural Technology Co., Ltd.); BBM insect olfactometer (Tai'an Baibo Instrument Co., Ltd.); Climacell 404 (ECO) artificial climate chamber; LT5001E electronic balance (sensitivity 0.1g, Changshu Tianliang Instrument Co., Ltd.); BSA2245-CW electronic balance (sensitivity 0.000 1g, Sartorius, Germany); KQ-700DB CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); quantitative pipette.

[0054] Test methods

[0055] Trapping plate test method

[0056] (1) Test method for trapping plate selection device

[0057] The prepared attractant was tested in a simulated experimental chamber. Under the conditions of 28±2℃ and 70±5% relative humidity, 50-100 adult stored pests were placed in a closed artificial climate of fixed size, and the number of adults on the trapping board was measured for 24 hours. A control test was set up to determine the attractant effect. A 25mm long conventional cigarette filter stick was glued to the trapping board, 1-2ml of tobacco flavor monomer was sucked with a quantitative pipette and dripped on the filter stick, which was hung in a simulated experimental chamber. Water was dripped on the filter stick of the blank trapping board as a control. The distribution of the test insects on the trapping board was observed after 24 hours of exposure, and the test was repeated 3 times.

[0058] (2) Comparative calculation of the trend effect of tobacco beetle adults. The specific calculation method and data analysis are as follows:

[0059] Attraction rate (%) = (number of insects attracted by treatment - number of insects in control) * 100 / total number of insects tested

[0060] Insect olfactometer test method:

[0061] (1) The two arms of the "Y"-shaped insect olfactometer are connected to two pear-shaped bottles respectively. The pear-shaped bottles contain tobacco flavor monomers. Before the airflow enters the pear-shaped bottles, it is first filtered with activated carbon to purify the air, and the air is humidified by a device that filters the air with water; the air flow rate is controlled between 100-120mL / min; the experiment needs to be carried out in the dark, and a fluorescent tube wrapped in red cloth is installed about 300mm above the olfactometer. During the experiment, one of the pear-shaped bottles of the two-arm olfactometer is set as the test odor source, and the other pear-shaped bottle is blank as a control; then release tobacco beetles from the center of the activity room, and observe and record the behavior choices of the insects at the set time points; the test insects that crawl within 100mm of the air inlet of the arm are regarded as test insects with behavioral choice responses, and the rest are regarded as test insects without behavioral choice responses. Each treatment is repeated 3 times.

[0062] (2) According to the calculation method of repellency coefficient of You et al., the calculation formula of attraction index is determined as follows:

[0063] Attraction rate EPI (%) = (number of insects attracted by treatment - number of insects in control) * 100 / total number of insects tested

[0064] AI (%) = [(TC) / (T+C)] × 100

[0065] Where: AI is the attraction index; T is the number of adult tobacco beetles in the treatment, head; C is the number of adult tobacco beetles in the control, head. If AI is negative, it means repellent effect.

[0066] 1. Comparison of the attractant effect of tobacco monomer flavors

[0067] This experiment was based on the complexity of the operation of the insect olfactometer and the poor selection effect of tobacco beetles in the test. Through comparative test methods, 17 types of purchased tobacco monomer flavors were tested in the simulated experimental chamber, and the trap plate selection device was used to conduct a preliminary screening of the tobacco beetle attracting effect. The results are shown in Table 1.

[0068] Table 1. Comparison of the attractant effects of tobacco monomer flavors

[0069]

[0070] As can be seen from Table 1, 17 flavor monomers were initially screened out from the tobacco flavor library, including alcohols, lipids, ketones, hydrocarbon compounds, etc., and 5 flavor monomers with better attractant effects were screened out, namely: phenylethyl acetate (1#), 3-phenylpropyl acetate (2#); (9Z,12E)-9,12-tetracyclopentadiene acetate (3#), 3-phenylpropyl butyrate (4#), and 3-phenylpropyl isobutyrate (5#).

[0071] (2) Attraction effects of different concentrations of five fragrance monomers

[0072] The insect olfactometer method was used to test 1#-5# at 2.5nL / cm 2 、12.5nL / cm 2 、25.5nL / cm 2 The behavioral selection response under concentration conditions was 16 tobacco beetle adults, and the distribution of tobacco beetles in the "Y" type insect olfactometer was observed 30 minutes after exposure. The test operation was repeated 3 times, and the attraction index was calculated to determine the behavioral selection response of tobacco beetle adults to different concentration gradients of 5 fragrance monomers. The results are shown in Table 2. It can be seen from Table 2 that the attraction index of fragrance monomers is different under different concentration gradients. The attraction effect is not obvious when the concentration is too high or too low. The medium concentration is 12.5nL / cm 2 The luring effect is better.

[0073] Table 2. Comparative analysis of the attraction index of five fragrance monomers at different concentration gradients

[0074]

[0075] 2. Comparison of the attractant effects of tobacco monomer flavors

[0076] Five flavor monomers were screened out, and the compound effects on the attraction of tobacco beetles were analyzed using the insect olfactometer method, and two compound formulas with the highest attraction index were screened out.

[0077] The insect olfactometer method was used to test tobacco beetle adults. The mass ratios of the components in the complex were 1:1, 1:1:1, 1:1:1:1 or 1:1:1:1:1. At 12.5 nL / cm2 The behavioral selection response under the concentration conditions was observed 30 minutes after exposure to the insects. The distribution of tobacco beetles in the "Y" type insect olfactometer was observed. The test operation was repeated 3 times, and the attraction index was calculated to determine the behavioral selection of tobacco beetles adults to the five fragrance monomers and compound fragrances. The results are shown in Table 3.

[0078] Table 3 Behavior selection of tobacco beetle adults on five tobacco monomer compound flavors

[0079]

[0080]

[0081] As can be seen from Table 3, after the compound attractant effect test, two kinds of phenylethyl acetate (1#) and 3-phenylpropyl acetate (2#), phenylethyl acetate (1#) and (9Z,12E)-9,12-tetracyclopentadiene acetate (3#) were screened out, and the attractant indexes of the compounds 1#*2# and 1#*3# reached 79.17% and 87.5%, respectively. Compared with other combinations, the attractant effect is slightly inferior. Therefore, the compound formulas 1#*2# and 1#*3# have the best attractant effect.

[0082] 3. Screening of different food attractant carrier formulations

[0083] The formula was treated with porous starch embedding, agar gel encapsulation, and silica gel pad sustained release. The residual period was analyzed by gas chromatography every 1 day, 3 days, and 5 days. The residual period refers to the time required for the content of tobacco flavor monomers to decrease by more than 75%. The test results in Table 5 show that the residual period of starch embedding is 1 day, the residual period of agar gel encapsulation is 3-5 days, and the silica gel pad sustained release can release the odor evenly for more than 5 days. The optimal food attractant carrier dosage form was determined, and the results are shown in Table 4.

[0084] Table 4. Comparative analysis of the residual effect of different food attractant carrier formulations

[0085]

[0086] 4. Comparison of the effects of combined use of tobacco flavors and sex hormones

[0087] The trap plate selection device test method was adopted. At a temperature of 28±2℃ and a relative humidity of 70±5%, 50 adult stored pests were placed in a sealed container of fixed size. The attractant effect of the fragrance monomer was tested in a simulated experimental chamber. The number of adults on the trap plate was measured for 24 hours, and a control test was set up to determine the attractant effect. The food attractant was used in combination with sex pheromone. The results showed that the relative attraction index of the combined use for tobacco beetles was greater than that of the sex pheromone alone. See Table 5 for details.

[0088] Table 5 Behavior selection of tobacco beetles adults to two tobacco monomer flavors and two compound monomer flavors

[0089]

[0090] It can be seen from Tables 1-5 that 17 flavor monomers were initially screened out from the tobacco flavor library, including alcohols, lipids, ketones, hydrocarbon compounds, etc., and 5 flavor monomers with an attraction index of 60%-65% were screened out through the olfactometer, namely: phenylethyl acetate, 3-phenylpropyl acetate; 3-phenylpropyl butyrate, 3-phenylpropyl isobutyrate, (9Z,12E)-9,12-tetracyclopentadiene acetate, among which phenylethyl acetate and 3-phenylpropyl acetate had an attraction index of 79% and 71%, respectively; after the compound attraction effect test, 2 compound formulas with an attraction index of 79.17% and 87.50% were screened out; after comparing the attraction effects of different concentrations of standard samples, the medium concentration of 12.5nL / cm 2 is better; according to the residual effect test of different carriers of food attractants, the silicone pad sustained release can release the odor evenly for more than 5 days, and its best food attractant carrier dosage form is determined; 2 kinds of fragrance monomers and 2 compound food attractants are selected for combination with sex pheromones, and the results show that the relative attraction index of the combination to tobacco beetles is greater than that of the sex pheromone alone.

[0091] Although the present invention has been disclosed as above by the embodiments, it is not intended to limit the present invention. Any person skilled in the art may make various choices and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention is limited by the claims and their equivalents.

Claims

1. A method for screening and compounding tobacco beetle food attractants using flavor monomers, characterized in that: The following steps are involved: S1. Preliminarily select M flavor monomer standards from the tobacco flavor monomer library, M≥15, and use a trapping plate selection device to preliminarily select attractants; S2. Use monomers with better attractant effects as the main ingredients, dilute them to a certain concentration, test the attractant effects with an insect olfactometer, determine the best attractant dosage, and compound the rest as auxiliary ingredients, select 1-2 formulas with better effects, and determine the compound dosage; S3. The monomer or formulation is treated with a variety of carrier dosage forms, wherein the carrier dosage form is porous starch embedding, agar gel encapsulation or silica gel pad sustained release, and the residual period is analyzed by gas chromatography to determine the best carrier dosage form.

2. The method for screening and compounding tobacco beetle food attractants using flavor monomers according to claim 1, characterized in that: In step S1, the initial selection of M kinds of fragrance monomer standards includes: M kinds of flavor monomers were initially screened out from the tobacco flavor library, M≥15. The flavor monomers included alcohols, lipids, ketones, ethers or hydrocarbon compounds. The 17 flavor monomers initially screened were: phenylethyl alcohol, methyl phenethyl ether, 3-phenylpropyl isobutyrate, 3-phenylpropyl butyrate, (9Z,12E)-9,12-tetracyclopentadiene acetate, 3-phenylpropyl acetate, cyclohexyl cinnamate, benzyl phenylacrylate, 3-phenylpropyl, phenylethyl acetate, dimethylbenzyl butyrate, amyl cinnamate, diphenylacetone, 3-phenylpropyl valerate, cis-3-vinyl cinnamate, megastigmatrienone and ethyl 2-methylbutyrate. The above flavor monomers were diluted to a certain concentration with n-hexane.

3. The method for screening and compounding tobacco beetle food attractants using flavor monomers according to claim 1, characterized in that: In step S1, the cultivation of the test tobacco beetle for primary selection of attractants includes: Feed: 90% whole wheat flour mixed with 10% yeast powder; Feeding conditions: temperature (28±2)℃, relative humidity (70±5)%; Rearing method: Add 1 / 3 volume of feed to a clean and dried glass jar (volume 375mL), take an appropriate number of tobacco beetle adults with normal behavior and place them on the surface of the feed; dig a hole with a diameter of 5cm in the bottle cap of the rearing jar, line the inner side of the cap with qualitative filter paper with the same diameter as the lid and seal the bottle, place it in an artificial climate box with a temperature of (28±2)℃ and a relative humidity of (70±5)% for lightless cultivation; rotate the insects every 7d to ensure the consistency of the test insect generations; before the test, pick tobacco beetle adults that have emerged for 7-14d, put them into an empty glass bottle without feed and starve them for 24h for use.

4. The method for screening and compounding tobacco beetle food attractants using flavor monomers according to claim 1, characterized in that: In step S1, the trapping method used for the primary attractant includes: (1) Test method for trapping plate selection device: At a temperature of 28±2℃ and a relative humidity of 70±5%, 100 tobacco beetle adults were placed in a sealed container of a fixed size, and the attracting effect of the flavor monomer was tested in a simulated experimental chamber, the number of adults on the trapping board was measured for 24 hours, and a control test was set up to determine the attracting effect; a 25mm long conventional cigarette filter stick was glued to the trapping board, 1-2ml of tobacco flavor monomer was sucked with a quantitative straw and dripped on the filter stick, and then hung in a simulated experimental chamber, and water was dripped on the filter stick of a blank trapping board as a control, and the distribution of the test insects on the trapping board was observed after 24 hours of exposure, and the test was repeated 3 times; (2) Comparative calculation of the trend effect of tobacco beetle adults. The specific calculation method and data analysis are as follows: Attraction rate (%) = (number of insects attracted by treatment - number of insects in control) * 100 / total number of insects tested.

5. The method for screening and compounding tobacco beetle food attractants using flavor monomers according to claim 1, characterized in that: In step S2, the attracting effect is tested using an insect olfactometer, wherein the insect olfactometer is a "Y"-shaped insect olfactometer, and the attracting index of the test includes: (1) The two arms of the "Y"-shaped insect olfactometer are connected to two pear-shaped bottles respectively. The pear-shaped bottles contain tobacco flavor monomers. Before the airflow enters the pear-shaped bottles, it is first filtered with activated carbon to purify the air, and the air is humidified by a device that filters the air with water; the air flow rate is controlled between 100-120mL / min; the experiment needs to be carried out in the dark, and a fluorescent tube wrapped in red cloth is installed about 300mm above the olfactometer; during the experiment, one of the pear-shaped bottles of the two-arm olfactometer is set as the test odor source, and the other pear-shaped bottle is blank as a control; then release tobacco beetle adults from the center of the activity room, and observe and record the insect's behavioral choices at the set time points; the test insects that crawl within 100mm of the air inlet of the arm are regarded as test insects with behavioral choice responses, and the remaining individuals are regarded as test insects without behavioral choice responses. Each treatment is repeated 3 times; (2) According to the calculation method of repellency coefficient, determine the calculation formula of attraction index: Attraction rate EPI (%) = (number of insects attracted by treatment - number of insects in control) * 100 / total number of insects tested AI (%) = [(TC) / (T+C)] × 100 In the formula: AI is the attraction index; T is the number of adult tobacco beetles in the treatment, heads; C is the number of adult tobacco beetles in the control, heads; if AI is a negative number, it means a repellent effect. Five fragrance monomers with relatively high attraction rates were screened, namely: hexyl acetate (1#), 3-phenylpropyl acetate (2#), (9Z,12E)-9,12-tetracyclopentadiene acetate (3#), 3-phenylpropyl butyrate (4#), and 3-phenylpropyl isobutyrate (5#).

6. The method for screening and compounding tobacco beetle feeding attractants using flavor monomers according to claim 1, characterized in that: In step S2, designing the optimal measurement includes: Select standard products with better attractant effects and dilute them to certain concentrations, such as 0.1%, 1%, 10%, and 100%, to determine the best attractant dosage; based on the best dosage, compound the products with the best attractant effect as the main component and the others as auxiliary components, screen a formula with better effects, and clarify the compound dosage.

7. The method for screening and compounding tobacco beetle food attractants using flavor monomers according to claim 1, characterized in that: In step S2, designing a compound formula includes: Five fragrance monomers with better attractant effects were preliminarily selected through the trapping plate, and the standard samples with better attractant effects were selected and diluted to a certain concentration to determine the optimal attractant dose; according to the optimal dose, the monomer with the best attractant effect was used as the main and the others as auxiliary to compound, and two of them were selected for compounding and screening, and the mass ratio of the material components in the compound was 1:1, 1:1:1, 1:1:1:1 or 1:1:1:1:1; the insect olfactometer was used to test the attraction index of the compound formula, a formula with better effect was screened, and the compound dosage was clarified.

8. The method for screening and compounding tobacco beetle food attractants using flavor monomers according to claim 1, characterized in that: In step S3, the determination of the optimal carrier dosage form includes: The formula was processed with porous starch embedding, agar gel encapsulation and silica gel pad sustained release. The residual effect period was analyzed by gas chromatography every 1d, 3d and 5d. Based on the residual effect period, silica gel pad sustained release was determined to be the best food attractant carrier dosage form.

9. The method for screening and compounding tobacco beetle food attractants using flavor monomers according to claim 8, characterized in that: The residual period refers to the time required for the content of tobacco flavor monomers to decrease by more than 75%. The residual time of attractants of different flavor monomer varieties is different. The residual period of attractants is generally 2-7 days.

10. A method for preparing a food attractant for improving the trapping efficiency of tobacco beetles, characterized in that: The following steps are involved: (1) Mixing fragrance monomers in proportion, wherein the fragrance monomers are phenylethyl acetate, 3-phenylpropyl acetate, 3-phenylpropyl butyrate, 3-phenylpropyl isobutyrate, and (9Z,12E)-9,12-tetracyclopentadiene acetate, preferably phenylethyl acetate and 3-phenylpropyl acetate are mixed in a mass ratio of 1:1 or phenylethyl acetate and (9Z,12E)-9,12-tetracyclopentadiene acetate are mixed in a mass ratio of 1:1; (2) Dilute the mixture to 12.5 nL / cm 2 The concentration was , and the diluted mixture was treated with a silica pad sustained release dosage form; (3) The treated food attractant is used in combination with sex pheromone to obtain a food attractant that improves the trapping efficiency of tobacco beetles.

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