Application of jasmonic acid synthesis inhibitor in promoting growth and development of rice

Abstract

The application belongs to the technical field of rice cultivation, and particularly relates to application of a jasmonic acid synthesis inhibitor in promoting growth and development of rice. The application finds that the jasmonic acid synthesis inhibitor can promote growth and development of rice, and specifically promotes growth and development of the aboveground part of rice and / or promotes growth and development of the root system of rice. The results of examples show that the jasmonic acid synthesis inhibitor, especially ibuprofen or copper reagent, can significantly increase the fresh weight of the aboveground part of rice, the root length and the fresh weight of the root, and the promoting effect of ibuprofen is superior to that of the copper reagent.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of rice cultivation, and particularly relates to application of a jasmonic acid synthesis inhibitor in promoting growth and development of rice. BACKGROUND

[0002] Root system is an important organ for rice to absorb water and nutrients, and is also an important place for synthesis and secretion of plant hormones, organic acids and other substances, and plays an important role in growth and development and yield formation of rice. Root system function is closely related to morphological and physiological characteristics of root system, and is jointly affected by genes and environmental factors. Good morphological and physiological characteristics of root system are a basic guarantee for maintaining crop yield. However, during rice cultivation or transplanting, the root system is usually greatly damaged, the root system activity is reduced, and the phenomena of dead seedlings and yellow seedlings are commonly caused. In general, the rice seedling recovery period is 3-4 days, if the rice seedling recovery is late, the recovery period is prolonged, and the tillering is late, which will affect the yield and quality of rice. Therefore, it is particularly important to promote growth and development of rice, especially to promote growth and development of rice root system, to improve the length of rice root system and / or the biomass of rice root system, and to improve the aboveground biomass of rice. SUMMARY

[0003] The application aims to promote growth and development of rice, especially to promote growth and development of rice root system, to improve the length of rice root system and / or the biomass of rice root system, and to improve the aboveground biomass of rice.

[0004] In order to achieve the above-mentioned purpose, the application provides application of a jasmonic acid synthesis inhibitor in promoting growth and development of rice, and the promoting growth and development of rice includes promoting growth and development of rice root system.

[0005] Preferably, the promoting growth and development of rice root system includes improving the length of rice root system and / or the biomass of rice root system.

[0006] Preferably, the promoting growth and development of rice also includes improving the aboveground biomass of rice.

[0007] Preferably, the jasmonic acid synthesis inhibitor includes ibuprofen and / or copper reagent.

[0008] Preferably, the action concentration of the ibuprofen is 20 muM; and the action concentration of the copper reagent is 5 muM.

[0009] The application also provides a method for promoting growth and development of rice, including the following steps: culturing rice seeds by using a culture solution containing a jasmonic acid synthesis inhibitor.

[0010] Preferably, the jasmonic acid synthesis inhibitor includes ibuprofen and / or copper reagent.

[0011] The action concentration of the ibuprofen in the jasmonic acid synthesis inhibitor is 20 muM.

[0012] The concentration of the copper reagent in the jasmonic acid synthesis inhibitor is 5 μM.

[0013] Preferably, the culture solution containing the jasmonic acid synthesis inhibitor comprises NH4Cl 395.83 g / 10L, Ca(NO3)2·4H2O 873.76 g / 10L, KNO3181.98 g / 10L, CaCl2886 g / 10L, MgSO4·7H2O 3240 g / 10L, K2SO4156.83 g / 10L, NaH2PO4·2H2O 403 g / 10L, Na2EDTA·2H2O 93.15 g / 10L, FeSO4·7H2O 69.65 g / 10L, MnCl2·4H2O 15.0 g / 10L, H3BO39.34 g / 10L, (NH 46 )·Mo7O 24 ·4H2O 0.74 g / 10L, ZnSO4·7H2O 0.35 g / 10L, CuSO4·5H2O 0.31 g / 10L, and the jasmonic acid synthesis inhibitor; when the jasmonic acid synthesis inhibitor is ibuprofen, the concentration is 20 μM; and when the jasmonic acid synthesis inhibitor is a copper reagent, the concentration is 5 μM.

[0014] Preferably, the method further comprises: planting the rice seeds after the rice seeds are cultured to have a root system of ≥12 cm.

[0015] Preferably, the rice seeds are rice seeds that are germinated with water.

[0016] Beneficial effects:

[0017] The present application provides application of a jasmonic acid synthesis inhibitor in promoting growth and development of rice. It is found that the jasmonic acid synthesis inhibitor can promote growth and development of rice, specifically, promote growth and development of the above-ground part of rice and / or promote growth and development of the root system of rice. The results of the examples show that the jasmonic acid synthesis inhibitor, especially ibuprofen or a copper reagent, can significantly increase the fresh weight of the above-ground part of rice and the root length and fresh weight of the root, and the promoting effect of ibuprofen is better than that of the copper reagent. BRIEF DESCRIPTION OF DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed in the embodiments will be briefly introduced below.

[0019] Fig. 1 Phenotypes of the rice planted in the greenhouse for 4 weeks in different treatment groups;

[0020] Fig. 2The growth and development of the above-ground and root of the rice in different treatment groups were planted in a greenhouse for 4 weeks; wherein, different letters represent significant difference between groups (P<0.05), and the same letter represents no significant difference. DETAILED DESCRIPTION

[0021] The application provides application of a jasmonic acid synthesis inhibitor in promoting growth and development of rice, which includes promoting above-ground growth and development of rice and / or promoting growth and development of rice root.

[0022] As an embodiment, the promoting above-ground growth and development of rice is to increase above-ground biomass of rice. In the application, the above-ground biomass of rice is fresh weight of rice above-ground. As an embodiment, the promoting growth and development of rice root includes increasing length and / or biomass of rice root; as another embodiment, the promoting growth and development of rice root includes increasing length and biomass of rice root. In the application, the biomass of rice root is fresh weight of rice root.

[0023] As an embodiment, the jasmonic acid synthesis inhibitor includes ibuprofen and / or copper reagent; as another embodiment, the jasmonic acid synthesis inhibitor is ibuprofen or copper reagent. The application does not have strict requirements for the source of the jasmonic acid synthesis inhibitor, which can be purchased conventionally. As an embodiment, the acting concentration of ibuprofen is 20 μM. As an embodiment, the acting concentration of copper reagent is 5 μM. The results of the embodiments of the application show that the above-ground biomass of rice treated by 20 μM ibuprofen is increased by 45.71% compared with the control group, and the length and biomass of rice root are increased by 61.66% and 104.76% respectively compared with the control group; the above-ground biomass of rice treated by 5 μM copper reagent (DIECA) is increased by 22.86% compared with the control group, and the length and biomass of rice root are increased by 20.76% and 61.90% respectively compared with the control group.

[0024] The application further provides a method for promoting growth and development of rice, which includes the following steps: culturing rice seeds by using a culture solution containing a jasmonic acid synthesis inhibitor.

[0025] As an embodiment, the application uses water to perform germination treatment on rice seeds to obtain germinated rice seeds. The application does not have strict requirements for the mode of the germination treatment, and a conventional step in the art can be adopted.

[0026] After obtaining the germinated rice seeds, the application uses a culture solution containing a jasmonic acid synthesis inhibitor to culture rice seeds to obtain cultured rice seeds.

[0027] In one embodiment, the jasmonic acid synthesis inhibitor includes ibuprofen and / or a copper reagent; in another embodiment, the jasmonic acid synthesis inhibitor is ibuprofen or a copper reagent. In one embodiment, when the jasmonic acid synthesis inhibitor is ibuprofen, the effective concentration of ibuprofen in the culture medium containing ibuprofen is 20 μM. In one embodiment, the culture medium containing ibuprofen comprises ibuprofen 20 μM, NH4Cl 395.83 g / 10 L, Ca(NO3)2·4H2O 873.76 g / 10 L, KNO3 181.98 g / 10 L, CaCl2 886 g / 10 L, MgSO4·7H2O 3240 g / 10 L, K2SO4 156.83 g / 10 L, NaH2PO4·2H2O 403 g / 10 L, Na2EDTA·2H2O 93.15 g / 10 L, FeSO4·7H2O 69.65 g / 10 L, MnCl2·4H2O 15.0 g / 10 L, H3BO3 9.34 g / 10 L, (NH4Cl)2·4H2O 15.0 ... 46 )·Mo7O 24 • 4H2O 0.74g / 10L, ZnSO4·7H2O 0.35g / 10L, CuSO4·5H2O 0.31g / 10L. As another embodiment, when the jasmonic acid synthesis inhibitor is a copper reagent, the effective concentration of the copper reagent in the culture medium containing the copper reagent is 5μM. In another embodiment, the culture medium containing copper reagent includes 5 μM copper reagent, 395.83 g / 10 L NH4Cl, 873.76 g / 10 L Ca(NO3)2·4H2O, 181.98 g / 10 L KNO3, 886 g / 10 L CaCl2, 3240 g / 10 L MgSO4·7H2O, 156.83 g / 10 L K2SO4, 403 g / 10 L NaH2PO4·2H2O, 93.15 g / 10 L Na2EDTA·2H2O, 69.65 g / 10 L FeSO4·7H2O, 15.0 g / 10 L MnCl2·4H2O, 9.34 g / 10 L H3BO3, and (NH4Cl)2·4H2O. 46 )·Mo7O 24 ·4H2O 0.74g / 10L, ZnSO4·7H2O 0.35g / 10L, CuSO4·5H2O 0.31g / 10L.

[0028] The application does not have strict requirements for the culture conditions, and routine operation is enough. As an embodiment, the application takes the culture of the germinated rice seeds to the root system ≥ 12 cm as the time node of the culture; as another embodiment, the application takes the culture of the germinated rice seeds to the root system ≥ 12 cm under normal culture conditions as the time node of the culture. The application limits the time node of the culture, which can promote the development of the root system earlier, and the developed root system can absorb relatively more nutrients, thereby promoting the overall development. As an embodiment, the application plants the rice seeds with the root system ≥ 12 cm.

[0029] As an embodiment, the seeds are planted in a greenhouse, and the conditions of the greenhouse planting are 30℃ in the daytime, 22℃ at night, light intensity of 3000 Lux, and light time of 12 hours.

[0030] In order to further illustrate the application, the application of the jasmonic acid synthesis inhibitor provided by the application in promoting the growth and development of rice is described in detail below in combination with the drawings and examples, but they cannot be understood as the limitation of the protection scope of the application.

[0031] Example 1

[0032] 1. Preparation of a jasmonic acid (JA) synthesis inhibitor solution

[0033] (1) Dissolve ibuprofen (IBU, purchased from GenoLeaf Biotech, CAS: 15687-27-1) in DMSO to prepare an IBU stock solution with a final concentration of 20 mM;

[0034] (2) Dissolve copper reagent (DIECA, sodium diethyldithiocarbamate, purchased from GenoLeaf Biotech, CAS: 20624-25-3) in DMSO to prepare a DIECA stock solution with a final concentration of 5 mM.

[0035] 2. Preparation of a rice nutrient solution

[0036] The rice nutrient solution is prepared from NH4Cl 395.83 g / 10 L, Ca(NO3)2·4H2O 873.76 g / 10 L, KNO3 181.98 g / 10 L, CaCl2 886 g / 10 L, MgSO4·7H2O 3240 g / 10 L, K2SO4 156.83 g / 10 L, NaH2PO4·2H2O 403 g / 10 L, Na2EDTA·2H2O 93.15 g / 10 L, FeSO4·7H2O 69.65 g / 10 L, MnCl2·4H2O 15.0 g / 10 L, H3BO3 9.34 g / 10 L, (NH4)6Mo7O24·4H2O 0.25 g / 10 L, and H2O 10 L. 46 24 ​• 4H20 0.74 g / 10 L, ZnS04-7H20 0.35 g / 10 L, CuS04-5H20 0.31 g / 10 L and the rest of water.

[0037] 3. Effects of JA synthesis inhibitors on the growth and development of rice

[0038] (1) Select rice seeds of uniform size, plump and intact.

[0039] (2) After the rice seeds of step (1) are germinated with water, select rice seeds with consistent growth and randomly divide them into 3 treatment groups for the following treatments:

[0040] Ibuprofen group (IBU): mix the rice nutrient solution of step 2 and the IBU mother liquor obtained in step 1 according to a certain volume ratio to make the final concentration of IBU 20 μM, and obtain the IBU culture solution; place the rice seeds in the IBU culture solution for cultivation;

[0041] Copper reagent group (DIECA): mix the rice nutrient solution of step 2 and the DIECA mother liquor obtained in step 1 according to a certain volume ratio to make the final concentration of DIECA 5 μM, and obtain the DIECA culture solution; place the rice seeds in the DIECA culture solution for cultivation

[0042] Control group (CK): mix the rice nutrient solution of step 2 and the same volume of DMSO as the IBU mother liquor of the ibuprofen group to obtain the normal culture solution; place the rice seeds in the normal culture solution for cultivation.

[0043] (3) When the rice of each treatment group in step (2) is cultivated to the appearance of phenotype (about 4 weeks), plant the rice of each treatment group in the greenhouse, and the operation mode of each treatment group is completely consistent except for the use of JA synthesis inhibitors. The greenhouse conditions are: 30°C during the day and 22°C at night, light intensity 3000 Lux, and light time 12 hours; after 4 weeks of greenhouse planting, observe the growth of rice, and respectively count the height of aboveground part (plant height) and the biomass of aboveground part (fresh weight of aboveground part), as well as the length of root system (root length) and the biomass (fresh weight of root) of each treatment group, and the results are shown in Figs. 1-2 and Table 1.

[0044] Table 1 Growth and development of rice aboveground and root system of each treatment group

[0045]

[0046] According to Figs. 1-2As can be seen from Table 1, after 4 weeks of greenhouse planting, the plant height of the above-ground part of the copper reagent group and the ibuprofen group has no obvious difference compared with the control group, the above-ground biomass of the ibuprofen group (IBU) is increased by 45.71% compared with the control group, the root length and the root biomass are increased by 61.66% and 104.76% respectively compared with the control group; the above-ground biomass of the copper reagent group (DIECA) is increased by 22.86% compared with the control group, the root length and the root biomass are increased by 20.76% and 61.90% respectively compared with the control group. The above-ground biomass, the root length and the root biomass of the ibuprofen group have significant difference (P<0.05) compared with the copper reagent group.

[0047] As can be seen from the above, the copper reagent and the ibuprofen can both improve the growth and development of the above-ground part of the rice and the growth and development of the root system of the rice, promote the fresh weight of the above-ground part of the rice and the length and the fresh weight of the root, and the promoting effect of the ibuprofen is superior to that of the copper reagent.

[0048] Although the above embodiment has made a detailed description of the present application, it is only a part of the embodiments of the present application, but not all the embodiments, and people can also obtain other embodiments according to the present embodiment without creativity, which all belong to the protection scope of the present application.

Claims

1. Application of jasmonic acid synthesis inhibitor in promoting growth and development of rice, the promoting growth and development of rice is to increase root length and root biomass of rice and to increase aboveground biomass of rice; the jasmonic acid synthesis inhibitor is ibuprofen or sodium diethyl dithiocarbamate; the action concentration of the ibuprofen is 20 μM; the action concentration of the sodium diethyl dithiocarbamate is 5 μM. The method comprises the following steps: culturing rice seeds by using culture solution containing jasmonic acid synthesis inhibitor; the culture solution containing jasmonic acid synthesis inhibitor comprises NH4Cl 395.83 g / 10L, Ca(NO3)2·4H2O 873.76 g / 10L, KNO3 181.98 g / 10L, CaCl2 886 g / 10L, MgSO4·7H2O 3240 g / 10L, K2SO4 156.83 g / 10L, NaH2PO4·2H2O 403 g / 10L, Na2EDTA·2H2O 93.15 g / 10L, FeSO4·7H2O 69.65 g / 10L, MnCl2·4H2O 15.0 g / 10L, H3BO3 9.34 g / 10L, (NH4)6·Mo7O24·4H2O 0.74 g / 10L, ZnSO4·7H2O 0.35 g / 10L, CuSO4·5H2O 0.31 g / 10L and jasmonic acid synthesis inhibitor; when the jasmonic acid synthesis inhibitor is ibuprofen, the concentration is 20 μM; when the jasmonic acid synthesis inhibitor is sodium diethyl dithiocarbamate, the concentration is 5 μM. The method further comprises: planting after the rice seeds are normally cultured to the root length ≥12 cm.

2. A method for promoting growth and development of rice, characterized by, The rice seeds are rice seeds germinated by water.

3. The method of claim 2, wherein, ​ 4. The method of claim 3, wherein, ​

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