Mouth wash and preparation method thereof

Abstract

The invention belongs to the technical field of mouthwash, and particularly relates to mouthwash and a preparation method thereof. The mouthwash provided by the invention comprises the following components in percentage by mass: 0.1-1% of an active component, 8-12% of a solubilizer, 8-12% of an anti-freezing agent, 4-6% of a preservative, 0.004-0.006% of an antioxidant, 4-6% of a bacteriostatic agent and the balance of water, the active component is an extracting solution of a mixture of radix inulae inula roots and rumex acetosa herbs; the mass ratio of the root of the Tibet inula root to the whole herb of the rumex acetosa is 8: (1.8-2.2). The mouthwash provided by the invention can effectively prevent and treat periodontitis, and has the advantages of convenience in use, high patient acceptability and good curative effect when being used for preventing and treating periodontitis.

Description

Technical Field

[0001] The invention belongs to the technical field of mouthwash, and particularly relates to a mouthwash and a preparation method thereof. Background Art

[0002] Chronic periodontitis is a common oral disease and an important factor causing gingival bleeding, periodontal tissue destruction, and tooth loss. Chronic periodontitis is a chronic inflammatory disease caused by the interaction between microorganisms and the host immune system. It causes persistent and irreversible damage to tooth support structures and is the primary cause of tooth loss.

[0003] Porphyromonas gingivalis (P.gingivalis, Pg) is a Gram-negative anaerobic coccobacillus present in the oral cavity and is one of the main pathogens causing periodontitis. Pg mainly occurs during gingival and periodontal infections, but its presence at low levels can also be found in 25% of healthy individuals without oral diseases. Pg is pathogenic and contains many toxic components, such as lipopolysaccharide, peptidoglycan and gingipains. Its most important toxic factor is a cysteine ​​protease called gingipains, which is composed of lysine-gingipains (lysine-gingipains, Kgp) and arginine-gingipains (arginine-gingipains, Rgp). These enzymes are essential for the survival and pathogenicity of Pg, and play a key role in host colonization, host defense inactivation, iron and nutrient acquisition, and tissue destruction. Gingipains are also the main virulence factor that enables Pg to evade host cell defense. For example, studies have found that in coronary artery endothelial cells, gingipains play a major role in preventing the interaction between Pg's autophagosomes and lysosomes, helping Pg to persist in the body. In addition, if periodontitis caused by Pg is not treated in time, it will lead to the occurrence or aggravation of other diseases. For example, Pg has been shown to be one of the risk factors for Alzheimer's disease (AD) and cognitive decline.

[0004] Clinically, periodontitis is usually treated with basic periodontal treatment (scaling, scaling, root planing, etc.) and anti-infective symptomatic treatment. However, the disease may recur after treatment, and improper use of periodontal treatment drugs may easily lead to adverse reactions and drug resistance. How to prevent and treat periodontitis simply and efficiently is a technical problem that needs to be solved urgently. Summary of the invention

[0005] In view of this, the present invention provides a mouthwash and a preparation method thereof. The mouthwash provided by the present invention can effectively prevent and treat the occurrence of periodontitis and is simple and easy to operate.

[0006] In order to solve the above technical problems, the present invention provides a mouthwash comprising the following components in percentage by mass:

[0007]

[0008] The active component is an extract of a mixture of the root of Tibetan woodruff and the whole plant of Rumex sylvestris; the mass ratio of the root of Tibetan woodruff to the whole plant of Rumex sylvestris is 8:1.8-2.2.

[0009] Preferably, the solubilizing agent comprises glycerol or hydrogenated castor oil;

[0010] The antifreeze agent includes sorbitol or ethylene glycol;

[0011] The preservatives include ethanol, sodium benzoate or potassium sorbate;

[0012] The antioxidant includes citric acid, sodium citrate or vitamin C;

[0013] The antibacterial agent includes caprylyl glycol, sodium sorbate, potassium sorbate or calcium propionate.

[0014] Preferably, the mass ratio of the Tibetan woodruff root to the whole plant of Rumex sylvestris is 8:2.

[0015] Preferably, the preparation method of the active component comprises the following steps:

[0016] Mixing the root of Aucklandia lappa, the whole plant of Rumex truncatum and the ethanol solution, and performing reflux extraction to obtain an extract;

[0017] Concentrating the extract to obtain an extract;

[0018] The extract is mixed with water and then defatted, decolorized, concentrated and dried in sequence to obtain the active component.

[0019] Preferably, the mass concentration of the ethanol solution is 85-95%.

[0020] Preferably, the mass ratio of the extract to water is 1:5-10.

[0021] Preferably, the defatting is performed by extracting a mixed system of the extract and water using petroleum ether.

[0022] Preferably, the volume ratio of the mixed system of the extract and water to petroleum ether is 1:0.8-1.2.

[0023] Preferably, the decolorization is carried out by mixing activated carbon and the degreased product for adsorption decolorization.

[0024] The present invention also provides a method for preparing the mouthwash described in the above technical solution, comprising the following steps:

[0025] The active component, solubilizer, antifreeze agent, preservative, antioxidant, antibacterial agent and water are mixed to obtain the mouthwash.

[0026] The present invention provides a mouthwash, comprising the following components in percentage by mass: 0.1-1% active component, 8-12% solubilizer, 8-12% antifreeze, 4-6% preservative, 0.004-0.006% antioxidant, 4-6% antibacterial agent and balance water; the active component is an extract of a mixture of the root of Tibetan wood rose and the whole plant of sorrel; the mass ratio of the root of Tibetan wood rose and the whole plant of sorrel is 8:1.8-2.2. The mouthwash provided by the present invention can effectively prevent and treat the occurrence of periodontitis, and the use of the mouthwash to prevent and treat periodontitis has the advantages of being easy to use, high patient acceptance and good efficacy. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a comparison chart of bacterial growth curves of the experimental group and the control group. DETAILED DESCRIPTION

[0028] The present invention provides a mouthwash comprising the following components in percentage by weight:

[0029]

[0030] The active component is an extract of a mixture of the root of Tibetan woodruff and the whole plant of Rumex sylvestris; the mass ratio of the root of Tibetan woodruff to the whole plant of Rumex sylvestris is 8:1.8-2.2.

[0031] Unless otherwise specified, all materials in the present invention are conventional commercially available products.

[0032] The mouthwash provided by the present invention comprises 0.1-1% active ingredient, preferably 0.3-0.8% by mass percentage. In the present invention, the active ingredient is an extract of a mixture of the root of Tibetan woodruff and the whole plant of Rumex truncatum. In the present invention, the preparation method of the active ingredient preferably comprises the following steps:

[0033] Mixing the root of Aucklandia lappa, the whole plant of Rumex truncatum and the ethanol solution, and performing reflux extraction to obtain an extract;

[0034] Concentrating the extract to obtain an extract;

[0035] The extract is mixed with water and then defatted, decolorized, concentrated and dried in sequence to obtain the active component.

[0036] The present invention mixes the root of Tibetan wood, the whole plant of Rumex truncatum and an ethanol solution, performs reflux extraction, and obtains an extract. In the present invention, the mixing preferably further comprises: crushing the root of Tibetan wood and the whole plant of Rumex truncatum respectively, and then sieving, and taking the sieve. In the present invention, the aperture of the sieve used for the sieve is 80 meshes. The present invention has no special requirements for the pulverization, and the conventional method in the field can be used.

[0037] In the present invention, the mass ratio of the root of Tibetan woodruff to the whole plant of Rumex truncatum is 8:1.8-2.2, preferably 8:2. In the present invention, the mass concentration of the ethanol solution is preferably 85-95%, more preferably 90%. The present invention has no special requirements for the amount of the ethanol solution, as long as the material can be immersed.

[0038] In the present invention, the number of reflux extractions is preferably 2 to 4 times, more preferably 3 times; the temperature of each reflux extraction is preferably 50 to 70°C, more preferably 55 to 60°C; the time of each reflux extraction is preferably 1 to 3 hours, more preferably 1.5 to 2 hours.

[0039] In the present invention, after the reflux extraction, the process preferably further comprises: filtering the system after the reflux extraction to obtain an extract. The present invention has no special requirements for the filtration, and conventional methods in the art can be used.

[0040] After obtaining the extract, the present invention concentrates the extract to obtain an extract. In the present invention, the concentration is preferably reduced pressure concentration. The present invention has no special requirements for the reduced pressure concentration.

[0041] After obtaining the extract, the present invention sequentially defatted, decolorized, concentrated and dried the extract and water to obtain the active component. In the present invention, the water is preferably deionized water. In the present invention, the mass ratio of the extract to water is preferably 1:5 to 10, more preferably 1:6 to 8. The present invention has no special requirements for the mixing, as long as it can be mixed evenly.

[0042] In the present invention, the degreasing is preferably performed by extracting the mixed system of extract and water with petroleum ether. In the present invention, the volume ratio of the mixed system of extract and water to petroleum ether is preferably 1:0.8-1.2, more preferably 1:1.

[0043] In the present invention, the decolorization is preferably carried out by mixing activated carbon and the degreased product for adsorption decolorization. In the present invention, the mass ratio of the activated carbon to the extract is preferably 1.8 to 2.2:1, more preferably 2:1.

[0044] The present invention has no special requirements for the concentration and drying, as long as the solvent in the system can be removed.

[0045] In the present invention, after drying, the method preferably further comprises: grinding the dried product to obtain the active component. In the present invention, the average particle size of the ground product is preferably 8 to 12 μm, more preferably 10 μm.

[0046] In the present invention, the active component can significantly inhibit the growth activity of Porphyromonas gingivalis which causes periodontitis.

[0047] In terms of mass percentage, the mouthwash provided by the present invention comprises 8-12% solubilizer, preferably 10%. In the present invention, the solubilizer preferably comprises glycerol or hydrogenated castor oil (PEG-40), more preferably glycerol. In the present invention, the solubilizer can enhance the solubility of the active component.

[0048] In terms of mass percentage, the mouthwash provided by the present invention comprises 8-12% antifreeze, preferably 10%. In the present invention, the antifreeze preferably comprises sorbitol or ethylene glycol, more preferably sorbitol.

[0049] The mouthwash provided by the present invention comprises 4-6% preservative in terms of mass percentage, preferably 5%. In the present invention, the preservative preferably comprises ethanol, sodium benzoate or potassium sorbate, more preferably ethanol.

[0050] In terms of mass percentage, the mouthwash provided by the present invention comprises 0.004-0.006% antioxidant, preferably 0.005%. In the present invention, the antioxidant preferably comprises citric acid, sodium citrate or vitamin C, more preferably sodium citrate.

[0051] The mouthwash provided by the present invention comprises 4-6% of antibacterial agent, preferably 5%, by mass percentage. In the present invention, the antibacterial agent preferably comprises caprylyl glycol, sodium sorbate, potassium sorbate or calcium propionate, more preferably caprylyl glycol.

[0052] The mouthwash provided by the present invention also includes the balance of water in terms of mass percentage. In the present invention, the water is preferably deionized water or distilled water, more preferably deionized water.

[0053] The present invention also provides a method for preparing the mouthwash described in the above technical solution, comprising the following steps:

[0054] The active component, solubilizer, antifreeze agent, preservative, antioxidant, antibacterial agent and water are mixed to obtain the mouthwash.

[0055] The present invention has no special requirements for the mixing, as long as the mixing can be uniform.

[0056] In order to further illustrate the present invention, the technical solutions provided by the present invention are described in detail below in conjunction with embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0057] Example 1

[0058] The root of Tibetan wood and the whole plant of sorrel are crushed respectively, and then sieved with a sieve with an aperture of 80 meshes, and the sieve is taken; the ground root of Tibetan wood, the ground whole plant of sorrel and an ethanol solution with a mass concentration of 90% are mixed, and reflux extraction is performed 3 times (each time reflux extraction is performed at 60° for 1.5 hours), and an extract is obtained by filtering; wherein the mass ratio of the ground root of Tibetan wood to the ground whole plant of sorrel is 8:2;

[0059] The extract is concentrated under reduced pressure to obtain an extract;

[0060] The extract and deionized water are mixed in a mass ratio of 1:7, and the mixed system is extracted with petroleum ether (the volume ratio of petroleum ether to the mixed system is 1:1) for degreasing; the degreased product and activated carbon are mixed in a mass ratio of 1:2 for adsorption decolorization, and the adsorption decolorization product is sequentially concentrated, dried and ground to obtain an active component with an average particle size of 10 μm;

[0061] The active component, glycerol, sorbitol, ethanol, sodium citrate, caprylyl glycol and deionized water are mixed to obtain a mouthwash; the mass percentage of each component in the mouthwash is 0.5% of the active component, 10% of glycerol, 10% of sorbitol, 5% of ethanol, 0.005% of sodium citrate and 5% of caprylyl glycol.

[0062] Example 2

[0063] A mouthwash was prepared according to the method of Example 1, except that the mass percentage of the active component in the mouthwash was 0.1%.

[0064] Example 3

[0065] A mouthwash was prepared according to the method of Example 1, except that the mass percentage of the active component in the mouthwash was 1%.

[0066] Comparative Example 1

[0067] A mouthwash was prepared according to the method of Example 1, except that no active component was added; specifically, glycerol, sorbitol, ethanol, sodium citrate, caprylyl glycol and deionized water were mixed to obtain a mouthwash; the mass percentage of each component in the mouthwash was 10% glycerol, 10% sorbitol, 5% ethanol, 0.005% sodium citrate and 5% caprylyl glycol.

[0068] The inhibitory activity of the mouthwash prepared in Examples 1 to 3 and Comparative Example 1 on Porphyromonas gingivalis was detected by the paper agar diffusion method. Test method: The -80°C frozen Porphyromonas gingivalis glycerol bacteria were prepared with Columbia liquid culture medium at a ratio of (1:100), and then placed in an anaerobic incubator (80% N2, 10% H2, 10% CO2) for culture. When the OD620nm of the Pg bacterial solution was measured by an ELISA instrument = 1.0, it was set aside. Purchase a special blank drug-sensitive sheet, place it in a 121°C high-pressure sterilizer for sterilization for 15 minutes, and then dry it for use. Add 50 μL of mouthwash to each drug-sensitive sheet and place it in a 37°C incubator to dry overnight. Divide the blood plate with a marker pen in advance, stick a group of drug concentrations of drug-sensitive paper on each blood plate, take 200uL of bacterial solution, evenly spread it on the blood plate with a disposable coating stick, and then stick the paper on the blood plate in each area according to the marked area. After leaving it for 15 minutes, put it in an anaerobic incubator at 37°C. After 72 hours, the formation of an inhibition zone was observed, and its diameter was measured and marked. There were 2 replicates for each drug concentration, and the whole experiment was performed 3 times. The average value was taken and the results are listed in Table 1.

[0069] Table 1 Diameter of inhibition zone of Pg by mouthwash of Examples and Comparative Examples (mm n=6)

[0070] Example Diameter of inhibition zone (mm) Example 1 22.43±1.37 Example 2 19.83±0.65 Example 3 26.42±0.78 Comparative Example 1 11.52±0.94

[0071] It can be seen from Table 1 that the mouthwash provided by the present invention has a good inhibitory activity against Porphyromonas gingivalis (Pg). The paper agar diffusion method shows that the inhibition zone of the mouthwash provided by the present invention against Porphyromonas gingivalis is 2 to 3 times that of Example 1.

[0072] The inhibitory activity of the mouthwash prepared in the embodiment on Porphyromonas gingivalis was detected according to the direct contact method. The specific detection method is: first add a mixture of 175 μL Columbia culture medium and 5 μL Porphyromonas gingivalis bacterial solution into a 96-well plate, and the experimental group adds the mouthwash prepared in Example 1; the control group is a normal bacterial culture without the addition of liquid. The volume of each well is 200 μL, and each group is repeated 3 wells, placed in an anaerobic box, and adjusted to 37°C. The OD620nm value of each group of mixed solutions at 0h, 8h, 12h, 24h and 48h is recorded respectively, and the results are listed in Table 2.

[0073] Table 2 OD620nm values ​​of the experimental group and the control group at different times

[0074]

[0075] According to Table 2, the bacterial growth curves of the experimental group and the control group were drawn. Figure 1 As shown. Combined Figure 1As can be seen from Table 2, the inhibitory activity of the mouthwash provided by the present invention on Porphyromonas gingivalis reaches 90% (180 μL bacterial solution + 20 μL mouthwash).

[0076] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A mouthwash, characterized in that: Includes the following components in percentage by mass: The active component is an extract of a mixture of the root of Tibetan woodruff and the whole plant of Rumex sylvestris; the mass ratio of the root of Tibetan woodruff to the whole plant of Rumex sylvestris is 8:1.8-2.

2.

2. The mouthwash according to claim 1, characterized in that: The solubilizing agent includes glycerin or hydrogenated castor oil; The antifreeze agent includes sorbitol or ethylene glycol; The preservatives include ethanol, sodium benzoate or potassium sorbate; The antioxidant includes citric acid, sodium citrate or vitamin C; The antibacterial agent includes caprylyl glycol, sodium sorbate, potassium sorbate or calcium propionate.

3. The mouthwash according to claim 1 or 2, characterized in that: The mass ratio of the Tibetan woodruff root to the whole plant of Rumex sylvestris is 8:

2.

4. The mouthwash according to claim 1, characterized in that: The preparation method of the active component comprises the following steps: Mixing the root of Aucklandia lappa, the whole plant of Rumex truncatum and the ethanol solution, and performing reflux extraction to obtain an extract; Concentrating the extract to obtain an extract; The extract is mixed with water and then defatted, decolorized, concentrated and dried in sequence to obtain the active component.

5. The mouthwash according to claim 4, characterized in that: The mass concentration of the ethanol solution is 85-95%.

6. The mouthwash according to claim 4, characterized in that: The mass ratio of the extract to water is 1:5-10.

7. The mouthwash according to claim 4, characterized in that: The degreasing is to extract the mixed system of extract and water by using petroleum ether.

8. The mouthwash according to claim 4, characterized in that: The volume ratio of the mixed system of the extract and water to petroleum ether is 1:0.8-1.

2.

9. The mouthwash according to claim 4, characterized in that: The decolorization is to mix the activated carbon and the degreased product for adsorption decolorization.

10. The method for preparing the mouthwash according to any one of claims 1 to 9, comprising the following steps: The active component, solubilizer, antifreeze agent, preservative, antioxidant, antibacterial agent and water are mixed to obtain the mouthwash.

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