Medicinal and edible composition and application thereof

Abstract

The invention discloses a medicinal and edible composition and application thereof. The invention provides a traditional Chinese medicine composition, which is prepared from the following traditional Chinese medicines in parts by weight: 1 to 3 parts of herba dendrodii officinalis, 0.2 to 0.3 part of semen ziziphi spinosae and 1 to 3 parts of rhizoma gastrodiae. The gastrodia elata can be replaced by radix ophiopogonis, and the radix ophiopogonis is 1-3 parts by weight. The medicinal and edible composition can achieve at least one of the following effects of treating and resisting anxiety, improving sleep and enhancing immunity.

Description

Technical Field

[0001] The invention relates to a medicine-food homologous composition and application thereof. Background Art

[0002] Anxiety disorder is one of the most common mental health problems in the world. According to data from the World Health Organization (WHO), about 3.6% of the world's population suffers from anxiety disorder. Studies have shown that anxiety disorder and insomnia often coexist, and the comorbidity rate of the two is quite high. It is estimated that about 50-90% of patients with anxiety disorder have sleep disorders, especially insomnia. A large proportion of insomnia patients also suffer from anxiety disorder, especially those with long-term insomnia are more likely to develop anxiety disorder.

[0003] Modern medicine has gradually realized that the treatment of anxiety and insomnia should adopt a multidisciplinary approach. The combination of various methods such as drug therapy, psychotherapy, behavioral intervention, and traditional Chinese medicine treatment can help improve the patient's physical and mental health more comprehensively. Among them, the application of traditional Chinese medicine in the treatment of anxiety and insomnia has also received more and more attention. Traditional Chinese medicine treatment of anxiety and insomnia usually combines traditional Chinese medicine, acupuncture, massage, and food therapy with the same origin of medicine and food, comprehensively regulating and treating both the symptoms and the root causes.

[0004] According to traditional Chinese medicine, anxiety and insomnia are usually related to dysfunction of the heart, liver, spleen, kidney and other organs. Anxiety is mostly caused by liver qi stagnation and restlessness, while insomnia is closely related to factors such as restlessness and imbalance of yin and yang. Clinical studies have shown that Chinese herbal compound has significant therapeutic effects on anxiety and insomnia, with fewer side effects and higher safety. Modern pharmacological studies have shown that certain ingredients in traditional Chinese medicine (such as jujube seed saponins in jujube seeds) have the effects of regulating the central nervous system, anti-anxiety, and improving sleep. The concept of "medicine and food are of the same origin" in traditional Chinese medicine emphasizes the prevention and treatment of diseases through daily diet. For the treatment of anxiety and insomnia, diet therapy focuses on regulating the functions of the whole body's organs and restoring the balance of yin and yang. The conditioning methods are mainly based on nourishing yin and clearing heat, soothing the liver and relieving depression, and calming the mind and tranquilizing the nerves.

[0005] With the global promotion of traditional Chinese medicine, the concept of medicine and food being of the same origin has gradually been recognized by people all over the world. Through its unique theoretical system and practical experience, traditional Chinese medicine provides a way to regulate the whole body and treat both the symptoms and the root causes in the treatment of anxiety and insomnia. The combination of drug treatment and diet therapy, as well as personalized syndrome differentiation and treatment, has enabled traditional Chinese medicine to show a broad application prospect in the management of anxiety and insomnia. Summary of the invention

[0006] The present invention provides a medicine-food homologous composition and its application based on traditional Chinese medicine health-preserving theory and combined with modern medical research. The present invention provides a medicine-food homologous composition, which can achieve at least one of the following effects: treating anxiety, improving sleep, and enhancing immunity.

[0007] The invention provides a traditional Chinese medicine composition, which comprises the following traditional Chinese medicines in parts by weight: 1-3 parts of dendrobium officinale, 0.2-0.3 parts of spiny jujube seeds, and 1-3 parts of gastrodia elata; the gastrodia elata can be replaced by ophiopogon japonicus, and in parts by weight, the ophiopogon japonicus is 1-3 parts.

[0008] In certain preferred embodiments of the present invention, the components in the Chinese medicine composition are defined as follows, and the components not mentioned are the same as those described in any scheme of the present invention (referred to as "a certain scheme").

[0009] In a certain embodiment, the Chinese medicine composition includes the following Chinese medicines in parts by weight: 1-3 parts of Dendrobium officinale, 0.2-0.3 parts of Ziziphus jujuba seeds, 1-3 parts of Gastrodia elata, 0.8-1.6 parts of Polygonatum odoratum, 0.6-1 parts of Honeysuckle, 0.5-0.9 parts of Cistanche deserticola, and 0.2-0.3 parts of Chrysanthemum; the Gastrodia elata can be replaced with Ophiopogon japonicus, and the Ophiopogon japonicus is 1-3 parts in parts by weight.

[0010] In a certain embodiment, the Chinese medicine composition is composed of 1-3 parts of Dendrobium officinale, 0.2-0.3 parts of Semen Ziziphi Spinosae and 1-3 parts of Gastrodia elata by weight, for example, 2 parts of Dendrobium officinale, 0.25 parts of Semen Ziziphi Spinosae and 1.5 parts of Gastrodia elata.

[0011] In a certain embodiment, the Chinese medicine composition is composed of 1-3 parts of Dendrobium officinale, 0.2-0.3 parts of Semen Ziziphi Spinosae and 1-3 parts of Radix Ophiopogonis by weight, for example, 2 parts of Dendrobium officinale, 0.25 parts of Semen Ziziphi Spinosae and 1.5 parts of Radix Ophiopogonis.

[0012] In a certain embodiment, the Chinese medicine composition is composed of the following Chinese medicines in parts by weight: 1-3 parts of Dendrobium officinale, 0.2-0.3 parts of Ziziphus jujuba seeds, 1-3 parts of Gastrodia elata, 0.8-1.6 parts of Polygonatum odoratum, 0.6-1 parts of Honeysuckle, 0.5-0.9 parts of Cistanche deserticola, and 0.2-0.3 parts of Chrysanthemum; the Gastrodia elata can be replaced with Ophiopogon japonicus, and in parts by weight, the Ophiopogon japonicus is 1-3 parts.

[0013] In a certain embodiment, the Chinese medicine composition is composed of 2 parts of Dendrobium officinale, 0.25 parts of Semen Ziziphi Spinosae and 1.5 parts of Gastrodia elata by weight.

[0014] In a certain embodiment, the Chinese medicine composition is composed of 2 parts of Dendrobium officinale, 0.25 parts of Semen Ziziphi Spinosae and 1.5 parts of Radix Ophiopogonis by weight.

[0015] In a certain embodiment, the Chinese medicine composition is composed of 2 parts of Dendrobium officinale, 0.25 parts of Ziziphus jujuba seeds, 1.5 parts of Gastrodia elata, 1.2 parts of Polygonatum odoratum, 0.8 parts of Honeysuckle, 0.7 parts of Cistanche deserticola, and 0.25 parts of Chrysanthemum, by weight.

[0016] In a certain embodiment, the Chinese medicine composition is composed of 2 parts of Dendrobium officinale, 0.25 parts of Ziziphus jujuba seeds, 1.5 parts of Ophiopogon japonicus, 1.2 parts of Polygonatum odoratum, 0.8 parts of Honeysuckle, 0.7 parts of Cistanche deserticola, and 0.25 parts of Chrysanthemum, by weight.

[0017] In the traditional Chinese medicine extract of the present invention, the Latin names of the raw materials used as active ingredients and their preparation methods can be found in the "Chinese Pharmacopoeia" (2020 edition, China Medical Science and Technology Press).

[0018] In the present invention, the Dendrobium officinale is the conventional medicinal Dendrobium officinale in the art, which is the dried stem of the orchid plant Dendrobium officinale Kimura et Migo. This product complies with the relevant provisions under Part I of the "Chinese Pharmacopoeia" (2020 edition); its processing method is the conventional processing method in the art, for example: the Dendrobium officinale is cut into segments, dried or low-temperature dried.

[0019] In the present invention, the Chinese jujube kernel is a conventional Chinese jujube kernel used as medicine in the field, which is the dried mature seed of the Rhamnaceae plant Chinese jujube (Ziziphusjujuba Mill. var. spinosa (Bunge) Hu ex HF Chou). This product complies with the relevant provisions under Part I of the "Chinese Pharmacopoeia" (2020 edition); its processing method is a conventional processing method in the field, for example: the Chinese jujube kernel is removed from the pulp and the kernel shell, the seeds are collected, and they are dried.

[0020] In the present invention, the Gastrodia elata is the Gastrodia elata conventionally used as medicine in the field, which is the dried tuber of the orchid plant Gastrodia elata (Gastrodiaelata Bl.). This product complies with the relevant provisions under the first item of the "Chinese Pharmacopoeia" (2020 edition); its processing method is the conventional processing method in the field, for example: the Gastrodia elata is washed, moistened or steamed to soften, cut into thin slices, and dried.

[0021] In the present invention, the Ophiopogon japonicus is the conventional Ophiopogon japonicus used as medicine in the art, which is the dried root tuber of the Liliaceae plant Ophiopogon japonicus (L. f) Ker-Gawl. This product complies with the relevant provisions under the first item of the "Chinese Pharmacopoeia" (2020 edition); its processing method is the conventional processing method in the art, for example: the Ophiopogon japonicus is washed, repeatedly exposed to the sun and stacked until it is 70% to 80% dry, the fibrous roots are removed, washed, moistened, flattened, and dried.

[0022] In the present invention, the Polygonatum odoratum is a conventional medicinal material in the field, which is the dried rhizome of the Liliaceae plant Polygonatum odoratum (Mill.) Druce. This product complies with the relevant provisions under the first item of the "Chinese Pharmacopoeia" (2020 edition); its processing method is a conventional processing method in the field, for example: the Polygonatum odoratum is removed from the fibrous roots, washed, and dried in the sun until soft, and then rubbed and dried repeatedly until there is no hard core, and dried in the sun; or steamed thoroughly, rubbed until translucent, and dried in the sun.

[0023] In the present invention, the honeysuckle is a conventional medicine in the art, which is a dried flower bud or a flower with initial opening of the honeysuckle plant Lonicerajaponica Thunb. of the Caprifoliaceae family. This product complies with the relevant provisions of the first item of the Chinese Pharmacopoeia (2020 edition). Its processing method is a conventional processing method in the art, for example: the drying of the honeysuckle.

[0024] In the present invention, the Cistanche deserticola is a conventional medicinal herb in the art, which is a dried fleshy stem with scale leaves of Cistanche deserticola YC Ma or Cistanche tuhulosa (Schenk) Wight of the Orobanchaceae family. This product complies with the relevant provisions of the first item of the Chinese Pharmacopoeia (2020 edition). Its processing method is a conventional processing method in the art, for example: the Cistanche deserticola is freed of impurities, washed, moistened, cut into thick slices, and dried. Irregular thick slices.

[0025] In the present invention, the chrysanthemum is a conventional medicinal chrysanthemum in the art, which is a dried inflorescence of the chrysanthemum (Chrysanthemummorifolium Ramat.) of the Asteraceae family. This product complies with the relevant provisions of the first item of the Chinese Pharmacopoeia (2020 edition). Its processing method is a conventional processing method in the art, for example: the chrysanthemum is shade-dried or baked, or fumigated, steamed and then sun-dried.

[0026] The present invention also provides an application of the Chinese medicine composition in a medicine for treating one or more of antianxiety, improving sleep and enhancing immunity.

[0027] The present invention also provides a medicine-food composition, which comprises a Dendrobium officinale extract, a Ziziphus jujuba seed extract, a Gastrodia elata extract or a Radix Ophiopogonis extract.

[0028] In a certain embodiment, it also includes polygonatum odoratum extract, honeysuckle extract, cistanche extract, and chrysanthemum extract.

[0029] In one embodiment, a sweetener and / or a sour agent is also included.

[0030] In the present invention, the weight proportions and sources of the Dendrobium officinale used in the Dendrobium officinale extract, the Ziziphus jujuba used in the Ziziphus jujuba seed extract, the Gastrodia elata used in the Gastrodia elata extract, the Ophiopogon japonicus used in the Ophiopogon japonicus extract, the Polygonatum odoratum used in the Polygonatum odoratum extract, the Lonicera japonica used in the Lonicera japonica extract, the Cistanche deserticola used in the Cistanche deserticola extract, and the Chrysanthemum used in the Chrysanthemum extract are the same as those described above.

[0031] In the present invention, the Dendrobium officinale extract is a conventional Dendrobium officinale extract in the art, which refers to an active substance extracted from the dried stems of Dendrobium officinale Kimura et Migo, a plant of the genus Dendrobium in the Orchidaceae family.

[0032] The main components contained in the Dendrobium officinale extract include: polysaccharide compounds, polyphenol compounds, flavonoid compounds, alkaloids and amino acids.

[0033] The extract of Dendrobium officinale can be obtained by conventional extraction methods in the art, such as water extraction, ultrasonic extraction, microwave-assisted extraction, etc.

[0034] In the present invention, hot water extraction method (using water as a solvent to extract water-soluble components such as polysaccharides in Ophiopogon japonicus by decoction, soaking, etc.) is preferred.

[0035] In a certain embodiment, the main active substance of the Dendrobium officinale extract is Dendrobium officinale polysaccharide.

[0036] In the present invention, the Chinese jujube seed extract is a conventional Chinese jujube seed extract in the art, which refers to an active substance extracted from the dried mature seeds of the Rhamnaceae plant Chinese jujube (Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex HF Chou).

[0037] The main components contained in the Chinese date seed extract include saponin, flavonoids, polysaccharides and alkaloids.

[0038] The Chinese date seed extract can be obtained by conventional extraction methods in the art, such as water extraction, organic solvent extraction (alcohol extraction), ultrasound-assisted extraction, enzyme extraction, microwave-assisted extraction, and supercritical fluid extraction.

[0039] In the present invention, hot water extraction method (using water as a solvent to extract water-soluble components in Ophiopogon japonicus, such as some saponins, etc., by decoction, soaking, etc.) is preferred.

[0040] In a certain embodiment, the main active ingredients of the jujube seed extract are jujube saponin A and spinosaponin.

[0041] In the present invention, the Gastrodia elata extract is a conventional Gastrodia elata extract in the art, which refers to an active substance extracted from the dried tuber of the orchid plant Gastrodia elata Bl.

[0042] The main components contained in the Gastrodia elata extract include: phenolic components, gastrodin derivatives, phenolic glycoside compounds, etc. The Gastrodia elata extract can be obtained by conventional extraction methods in the art, such as water extraction, organic solvent extraction (alcohol extraction), ultrasound-assisted extraction, enzyme extraction, microwave-assisted extraction and supercritical fluid extraction;

[0043] In the present invention, hot water extraction method (using water as a solvent to extract water-soluble components in Ophiopogon japonicus, such as some saponin compounds, etc., by decoction, soaking, etc.) is preferred.

[0044] In a certain embodiment, the main active ingredients of the Gastrodia elata extract are gastrodin, p-hydroxybenzyl alcohol, balisonoside E, balisonoside B, balisonoside C and balisonoside.

[0045] In the present invention, the Ophiopogon japonicus extract is a conventional Ophiopogon japonicus extract in the art, which refers to an active substance extracted from the dried root tuber of Ophiopogon japonicus (L. f) Ker-Gawl.

[0046] The main components contained in the Ophiopogon japonicus extract include: steroidal saponins (such as Ophiopogon japonicus saponins A, B, C, D, etc.), polysaccharide compounds (such as Ophiopogon japonicus polysaccharide), flavonoids, amino acids, volatile oils, etc.

[0047] The Ophiopogon japonicus extract can be obtained by conventional extraction methods in the art, such as water extraction, organic solvent extraction (alcohol extraction), ultrasound-assisted extraction, enzyme extraction, microwave-assisted extraction, and supercritical fluid extraction.

[0048] In the present invention, hot water extraction method (using water as a solvent to extract water-soluble components in Ophiopogon japonicus, such as polysaccharides and some saponin compounds, by decoction, soaking, etc.) is preferred.

[0049] In a certain embodiment, the main active substances of the Ophiopogon japonicus extract are methyl ophiopogon isoflavone A, methyl ophiopogon isoflavone B and total saponins of Ophiopogon japonicus.

[0050] In the present invention, the Polygonatum odoratum extract is a conventional Polygonatum odoratum extract in the art, which refers to an active substance extracted from the dried rhizome of Polygonatum odoratum (Mill.) Druce.

[0051] The main components contained in the polygonatum odoratum extract include: polysaccharide compounds (such as polygonatum odoratum polysaccharide), saponin compounds (such as steroidal saponin), flavonoid compounds, amino acids, vitamins and minerals.

[0052] The polygonatum odoratum extract can be obtained by conventional extraction methods in the art, such as water extraction, organic solvent extraction (alcohol extraction), ultrasound-assisted extraction, enzyme extraction, microwave-assisted extraction, and supercritical fluid extraction;

[0053] In the present invention, hot water extraction method (using water as a solvent to extract water-soluble components in Polygonatum odoratum, such as polysaccharide compounds, by decoction, soaking, etc.) is preferred.

[0054] In a certain embodiment, the main active substance of the Polygonatum odoratum extract is Polygonatum odoratum polysaccharide.

[0055] In the present invention, the honeysuckle extract is a conventional honeysuckle extract in the art, which refers to an active substance extracted from the dried flower buds or fresh flowers of Lonicera japonica Thunb.

[0056] The main components contained in the honeysuckle extract include: flavonoids (such as luteolin, luteolin, chrysanthemin, etc.), saponin compounds, volatile oils (such as terpenoids), organic acids (such as chlorogenic acid, caffeic acid, etc.), and polysaccharide compounds.

[0057] The honeysuckle extract can be obtained by conventional extraction methods in the art, such as water extraction, organic solvent extraction (alcohol extraction), ultrasound-assisted extraction, enzyme extraction, microwave-assisted extraction, and supercritical fluid extraction;

[0058] In the present invention, hot water extraction method (using water as a solvent to extract water-soluble components in honeysuckle, such as saponin compounds, polysaccharide compounds and some organic acids, by decoction, soaking, etc.) is preferred.

[0059] In a certain embodiment, the main active ingredients of the honeysuckle extract are chlorogenic acid and luteolin.

[0060] In the present invention, the Cistanche deserticola extract is a conventional Cistanche deserticola extract in the art, which refers to an active substance extracted from the dried fleshy stems with scale leaves of Cistanche deserticola YC Ma or Cistanche tuhulosa (Schenk) Wight, plants of the Orobanchaceae family.

[0061] The main components contained in the Cistanche extract include: flavonoids, phenylethanol glycosides (such as Cistanche glycosides A, B, C, D, etc.), volatile components (such as terpenoids), polysaccharides, trace elements, etc.

[0062] The Cistanche deserticola extract can be obtained by conventional extraction methods in the art, such as water extraction, organic solvent extraction (alcohol extraction), ultrasound-assisted extraction, enzyme extraction, microwave-assisted extraction, and supercritical fluid extraction.

[0063] In the present invention, the hot water extraction method (using water as a solvent to extract water-soluble components in Cistanche deserticola, such as saponin compounds and polysaccharide compounds, by decoction, soaking, etc.) is preferred.

[0064] In a certain embodiment, the main active substances of the Cistanche deserticola extract are echinacoside and verbascoside.

[0065] In the present invention, the chrysanthemum extract is a conventional chrysanthemum extract in the art, which refers to an active substance extracted from the flowers of chrysanthemum (Chrysanthemum morifolium Ramat.).

[0066] The main components contained in the chrysanthemum extract include: flavonoids (including quercetin, kaempferol, luteolin, etc.), volatile components (such as terpenoids), organic acids (such as chlorogenic acid, caffeic acid, etc.), polysaccharides, trace elements, etc.

[0067] The chrysanthemum extract can be obtained by conventional extraction methods in the art, such as water extraction, organic solvent extraction (alcohol extraction), ultrasound-assisted extraction, enzyme extraction, microwave-assisted extraction, and supercritical fluid extraction;

[0068] In the present invention, hot water extraction method (using water as a solvent to extract water-soluble components in chrysanthemum, such as polysaccharides, some organic acids and some flavonoids, etc., by decoction, soaking, etc.) is preferred.

[0069] In a certain embodiment, the main active substances of the chrysanthemum extract are chlorogenic acid, luteolin and 3,5-O-dicaffeoylquinic acid.

[0070] In the present invention, the sweetener may be a conventional sweetener in this type of composition in the art, such as one or more of white sugar, glucose, sugar alcohols, sucralose, aspartame, acesulfame potassium, and stevioside, and further comprises white sugar.

[0071] In the present invention, the acidulant may be a conventional acidulant in the composition of this type in the art, such as one or more of citric acid, malic acid, and tartaric acid, and further, for example, citric acid.

[0072] In a certain embodiment, the medicine-food composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, and the Gastrodia elata extract.

[0073] In a certain embodiment, the Brix value (Japanese ATAGO saccharimeter) of the medicine-food composition is 10%-25%, for example, 12.6%, 13.5%.

[0074] In a certain embodiment, the medicine-food composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, the Gastrodia elata extract, the Polygonatum odoratum extract, the Honeysuckle extract, the Cistanche deserticola extract, and the Chrysanthemum extract.

[0075] In a certain embodiment, the Brix value (Japanese ATAGO saccharimeter) of the medicine-food composition is 10%-25%, for example, 11.9%.

[0076] In a certain embodiment, the medicine-food composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, the Gastrodia elata extract, the Polygonatum odoratum extract, the Honeysuckle extract, the Cistanche deserticola extract, the Chrysanthemum extract, white sugar, and citric acid.

[0077] In a certain embodiment, the medicine-food composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, and the Ophiopogon japonicus extract.

[0078] In a certain embodiment, the Brix value (Japanese ATAGO saccharimeter) of the medicine-food composition is 10%-25%, for example, 13.1%, 13.9%.

[0079] In a certain embodiment, the medicine-food composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, the Ophiopogon japonicus extract, the Polygonatum odoratum extract, the Honeysuckle extract, the Cistanche deserticola extract, and the Chrysanthemum extract.

[0080] In a certain embodiment, the Brix value (Japanese ATAGO saccharimeter) of the medicine-food composition is 10%-25%, for example, 12.2%.

[0081] In a certain embodiment, the medicine-food composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, the Ophiopogon japonicus extract, the Polygonatum odoratum extract, the Honeysuckle extract, the Cistanche deserticola extract, the Chrysanthemum extract, white sugar, and citric acid.

[0082] In a certain embodiment, the medicine-food composition further comprises one or more of a preservative, an excipient and an antioxidant.

[0083] In the present invention, the antioxidant may be a conventional antioxidant in this type of composition in the art, such as one or more of L-ascorbic acid (vitamin C), sodium L-ascorbate, isoascorbic acid, sodium isoascorbate, tea polyphenols, vitamin E, and superoxide dismutase, and further such as L-ascorbic acid (vitamin C).

[0084] In the present invention, the preservative may be a conventional preservative in this type of composition in the art, such as one or more of sorbic acid, potassium sorbate, parabens, chitosan, and tea polyphenols, and further sorbic acid.

[0085] In the present invention, the excipient may be a conventional excipient in this type of composition in the art, such as one or more of maltodextrin, mannitol, lactose, soluble starch, and β-cyclodextrin, and further maltodextrin.

[0086] In a certain embodiment, the medicine-food composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, the Gastrodia elata extract, the Polygonatum odoratum extract, the Honeysuckle extract, the Cistanche deserticola extract, the Chrysanthemum extract, white sugar, citric acid, the preservative and the antioxidant.

[0087] In a certain embodiment, the medicine-food composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, the Ophiopogon japonicus extract, the Polygonatum odoratum extract, the Honeysuckle extract, the Cistanche deserticola extract, the Chrysanthemum extract, white sugar, citric acid, the preservative and the antioxidant.

[0088] In a certain embodiment, the medicine-food composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, the Gastrodia elata extract, the Polygonatum odoratum extract, the Honeysuckle extract, the Cistanche deserticola extract, the Chrysanthemum extract, white sugar, citric acid, the preservative, the excipient and the antioxidant.

[0089] In a certain embodiment, the medicine-food composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, the Ophiopogon japonicus extract, the Polygonatum odoratum extract, the Honeysuckle extract, the Cistanche deserticola extract, the Chrysanthemum extract, white sugar, citric acid, the preservative, the excipient and the antioxidant.

[0090] The present invention also provides a method for preparing the above-mentioned medicine-food homologous composition, which uses the above-mentioned traditional Chinese medicine composition and comprises the following steps:

[0091] S1: heating the weighed Chinese medicine composition with water, and filtering to obtain an extract 1 and a filter residue;

[0092] S2: heating the filter residue and water, filtering to obtain extract 2; combining the extract 1 and the extract 2, filtering to obtain a mixed extract;

[0093] S3: centrifuging and filtering the mixed extract, concentrating and sterilizing the intercepted liquid obtained by microfiltration to obtain a concentrated liquid, thereby obtaining the medicinal and edible composition.

[0094] In a certain embodiment, the preparation method further includes step S4: the concentrated solution and water are heated for the first time, and then heated for the second time with the sweetener, the acidulant, the antioxidant and the preservative to obtain the medicine-food composition.

[0095] In a certain embodiment, in S1, the mass ratio of the water to the traditional Chinese medicine composition is 12-15:1.

[0096] In one embodiment, in S1, the heating temperature is 90±5°C.

[0097] In one embodiment, in S1, the heating time is 120-90 minutes.

[0098] In a certain embodiment, in S2, the mass ratio of the water to the filter residue is 8-10:1.

[0099] In one embodiment, in S2, the heating temperature is 90±5°C.

[0100] In one embodiment, in S2, the heating time is 60-30 minutes.

[0101] In one embodiment, in S2, the filtering is screening, and the screening screen is 40-80 mesh, for example, 60 mesh.

[0102] In one embodiment, in S3, the centrifugation is performed using a tubular centrifuge.

[0103] In a certain scheme, in S3, the filtration is to filter the centrifuge liquid obtained by the centrifugation using a ceramic membrane; the membrane pore size of the ceramic membrane filtration is 0.2 μm; and the permeate flow rate of the ceramic membrane filtration is 60 L / hr.

[0104] In a certain scheme, in S3, the concentration is performed by using a reverse osmosis membrane; the molecular weight cutoff of the reverse osmosis membrane concentration is 100; and the permeate flow rate of the reverse osmosis membrane concentration is 90 L / hr.

[0105] In one embodiment, in S3, the Brix value of the concentrate is 10%-25%, for example, 11.9%.

[0106] In one embodiment, in S3, the sterilization is pasteurization.

[0107] In one embodiment, in S4, the mass ratio of the concentrate to the water is 8:3, for example, 16.08:6.

[0108] In one embodiment, in S4, the temperature of the first heating is 80°C.

[0109] In one embodiment, in S4, the sweetener is white sugar.

[0110] In one embodiment, in S4, the mass ratio of the concentrate to the sweetener is 16.08:1.8.

[0111] In one embodiment, in S4, the acidulant is citric acid.

[0112] In a certain embodiment, in S4, the mass ratio of the concentrate to the acidulant is 16.08:0.0015.

[0113] In one embodiment, in S4, the temperature of the second heating is 95±5°C.

[0114] In one embodiment, in S4, the second heating time is 10-20 minutes, for example, 15 minutes.

[0115] In a certain embodiment, in S4, water is added after the second heating; the mass ratio of the added water to the concentrated liquid is 30:16.08.

[0116] The present invention also provides a medicine-food composition obtained by the above preparation method.

[0117] The present invention also provides an application of the above-mentioned medicine-food homologous composition in a drug for treating one or more of anxiolysis, improving sleep and enhancing immunity.

[0118] In the prescription of the present invention, Dendrobium is used as the main drug, nourishing yin and clearing heat; it is supplemented with Semen Ziziphi Spinosae to calm the nerves and help sleep, Gastrodia elata to calm the liver and relieve wind (or Radix Ophiopogonis to nourish yin and moisten dryness), and the two drugs are collectively the ministerial drugs; Polygonatum odoratum strengthens nourishing yin and moistening dryness, and regulates the mind; Honeysuckle and Chrysanthemum clear away heat and detoxify, calm the liver and improve eyesight, so as to prevent heat and toxicity stagnation that may be caused by nourishing yin; the three drugs are collectively the adjuvant drugs; Cistanche deserticola nourishes the kidney and helps yang, so as to prevent yin nourishing drugs from damaging yang, and is the guiding drug. The raw materials in the whole prescription are all of the same origin as medicine and food, and they play the effects of nourishing yin and tonifying the kidney, clearing the liver and nourishing the heart, calming the nerves and helping sleep, achieving the effects of anti-anxiety, improving sleep and enhancing immunity, and perfectly embodying the profound mystery of traditional Chinese medicine and health care prescriptions. DETAILED DESCRIPTION

[0119] The present invention is further described below by way of examples, but the present invention is not limited to the scope of the examples. The experimental methods in the following examples without specifying specific conditions are carried out according to conventional methods and conditions, or selected according to the product specifications.

[0120] Example 1 Preparation of Composition 1

[0121] 1. Weigh 5 kg of Dendrobium officinale, 0.25 kg of Ziziphus jujuba seeds, and 2.5 kg of Gastrodia elata, and crush them with a hammer mill (model WFJ-15, Jiangyin Kanghe Machinery Manufacturing Co., Ltd.) and pass through a 5-mesh sieve for later use.

[0122] 2. Add the crushed material into a multifunctional dynamic extraction tank (model TT-500, Jining Jinbaite Bio-Machinery Co., Ltd.), add 12 times the amount of water as the raw material, turn on the steam and heat to 90°C, stir and extract for 90 minutes, and then the liquid is obtained as extract 1; for the second extraction, add 8 times the amount of water as the raw material, turn on the steam and heat to 90°C, stir and extract for 45 minutes, and then the liquid is obtained as extract 2. Combine extracts 1 and 2, pass through a 60-mesh sieve, and use reduced pressure concentration (-0.06Mpa~-0.08Mpa, 70±5°C) to obtain 7.75kg of concentrated solution with a Brix value (Japanese ATAGO saccharimeter) of 13.5%. After pasteurization, the concentrated solution is poured into a sterile bag for standby use.

[0123] Example 2 Preparation of Composition 2

[0124] 1. Weigh 2kg of Dendrobium officinale, 0.5kg of Semen Ziziphi Spinosae, and 3kg of Gastrodia elata, and crush them with a hammer mill to pass through a 5-mesh sieve, and set aside.

[0125] 2. Add the crushed material into the multifunctional dynamic extraction tank, add 12 times the amount of water of the raw material, turn on the steam and heat to 90°C, stir and extract for 90 minutes, and then the liquid is obtained as Extract 1; for the second extraction, add 8 times the amount of water of the raw material, turn on the steam and heat to 90°C, stir and extract for 45 minutes, and then the liquid is obtained as Extract 2. Combine Extracts 1 and 2, pass through a 60-mesh sieve, and use reduced pressure concentration (-0.06Mpa~-0.08Mpa, 70±5°C) to obtain 5.5kg of concentrated solution with a Brix value of 12.6%. After pasteurization, the concentrated solution is poured into a sterile bag for standby use.

[0126] Example 3 Preparation of Composition 3

[0127] 1. Weigh 5kg of Dendrobium officinale, 0.25kg of Semen Ziziphi Spinosae, and 2.5kg of Radix Ophiopogonis, and crush them with a hammer mill to pass through a 5-mesh sieve, and set aside.

[0128] 2. Add the crushed material into the multifunctional dynamic extraction tank, add 12 times the amount of water of the raw material, turn on the steam and heat to 90°C, stir and extract for 60 minutes, and then the liquid is obtained as Extract 1; for the second extraction, add 8 times the amount of water of the raw material, turn on the steam and heat to 90°C, stir and extract for 30 minutes, and then the liquid is obtained as Extract 2. Combine Extracts 1 and 2, pass through a 60-mesh sieve, and use reduced pressure concentration (-0.06Mpa~-0.08Mpa, 70±5°C) to obtain 7.75kg of concentrated solution with a Brix value of 13.9%. After pasteurization, the concentrated solution is poured into a sterile bag for standby use.

[0129] Example 4 Preparation of Composition 4

[0130] 1. Weigh 2kg of Dendrobium officinale, 0.5kg of Ziziphus jujuba seeds, and 3kg of Ophiopogon japonicus, and crush them with a hammer mill to pass through a 5-mesh sieve, and set aside.

[0131] 2. Add the crushed material into the multifunctional dynamic extraction tank, add 12 times the amount of water of the raw material, turn on the steam and heat to 90°C, stir and extract for 60 minutes, and then the liquid is obtained as Extract 1; for the second extraction, add 8 times the amount of water of the raw material, turn on the steam and heat to 90°C, stir and extract for 30 minutes, and then the liquid is obtained as Extract 2. Combine Extracts 1 and 2, pass through a 60-mesh sieve, and use reduced pressure concentration (-0.06Mpa~-0.08Mpa, 70±5°C) to obtain 5.5kg of concentrated solution with a Brix value of 13.1%. After pasteurization, the concentrated solution is poured into a sterile bag for standby use.

[0132] Example 5 Preparation of Composition 5

[0133] 1. Weigh 8kg of Dendrobium candidum, 1kg of Ziziphus jujuba seeds, 6kg of Gastrodia elata, 4.8kg of Polygonatum odoratum, 3.2kg of Honeysuckle, 2.8kg of Cistanche deserticola, and 1kg of Chrysanthemum, and crush them with a hammer mill to pass through a 5-mesh sieve, and set aside.

[0134] 2. Add the crushed material into a multifunctional dynamic extraction tank, add 12 times the amount of water as the raw material, turn on the steam and heat to 90°C, stir and extract for 60 minutes, and then the liquid is obtained as extract 1; for the second extraction, add 8 times the amount of water as the raw material, turn on the steam and heat to 90°C, stir and extract for 30 minutes, and then the liquid is obtained as extract 2. Combine extracts 1 and 2, pass through a 60-mesh sieve, and centrifuge with a tubular centrifuge. Pour the centrifuge into a ceramic membrane filtration equipment (membrane pore size 0.2µm, permeate flow rate 60L / hr) for microfiltration, collect the retentate, and concentrate the retentate using a reverse osmosis membrane (molecular weight cutoff 100, permeate flow rate 90L / hr) to obtain 26.8kg of concentrated solution with a Brix value of 11.9%. After pasteurization, the concentrate is poured into a sterile bag for standby use.

[0135] Example 6 Preparation of Composition 6

[0136] 1. Weigh 8kg of Dendrobium candidum, 1kg of Ziziphus jujuba seeds, 6kg of Ophiopogon japonicus, 4.8kg of Polygonatum odoratum, 3.2kg of Honeysuckle, 2.8kg of Cistanche deserticola, and 1kg of Chrysanthemum, and crush them with a hammer mill to pass through a 5-mesh sieve, and set aside.

[0137] 2. Add the crushed material into a multifunctional dynamic extraction tank, add 12 times the amount of water as the raw material, turn on the steam and heat to 90°C, stir and extract for 60 minutes, and then the liquid is obtained as extract 1; for the second extraction, add 8 times the amount of water as the raw material, turn on the steam and heat to 90°C, stir and extract for 30 minutes, and then the liquid is obtained as extract 2. Combine extracts 1 and 2, pass through a 60-mesh sieve, and centrifuge with a tubular centrifuge. Pour the centrifuge into a ceramic membrane filtration equipment (membrane pore size 0.2µm, permeate flow rate 60L / hr) for microfiltration, collect the retentate, and concentrate the retentate using a reverse osmosis membrane (molecular weight cutoff 100, permeate flow rate 90L / hr) to obtain 26.8kg of concentrated solution with a Brix value of 12.2%. After pasteurization, the concentrate is poured into a sterile bag for standby use.

[0138] Example 7 Preparation of Dendrobium and Gastrodia Drink

[0139] According to the formula ratio, 16.08 kg of the concentrated solution of composition 5 (equivalent to 16.08 kg of raw materials) was weighed and added to the batching tank, and 20% of the finished product amount of purified water (6 kg) was added, and the mixture was stirred and heated to 80°C to mix well. According to the formula ratio, 6% of the finished product amount of white sugar (1.8 kg) and 0.05% of the finished product amount of citric acid (1.5 g) were weighed, and the above white sugar and citric acid were added to the batching tank in turn, stirred to dissolve, and continued to heat to a slight boil (95±5°C) and maintained for 15 minutes, and purified water was added to the full amount, the total amount was 30 kg, stirred evenly, and filled to obtain about 1200 bottles of Dendrobium and Gastrodia Drink (25 mL / bottle), which were sterilized and set aside.

[0140] Example 8 Preparation of Dendrobium and Ophiopogon Drink

[0141] According to the formula ratio, 16.08 kg of the concentrated solution of composition 6 (equivalent to 16.08 kg of raw materials) was weighed and added to the batching tank, and 20% of the finished product amount of purified water (6 kg) was added, and the mixture was stirred and heated to 80°C to mix well. According to the formula ratio, 6% of the finished product amount of white sugar (1.8 kg) and 0.05% of the finished product amount of citric acid (1.5 g) were weighed, and the above white sugar and citric acid were added to the batching tank in turn, stirred to dissolve, and continued to heat to a slight boil (95±5°C) and maintained for 15 minutes, and purified water was added to the full amount, the total amount was 30 kg, stirred evenly, and filled to obtain about 1200 bottles of Dendrobium and Ophiopogon Drink (25 mL / bottle), which were sterilized and set aside.

[0142] Comparative Example 1 Preparation of Composition A

[0143] 1. Weigh 4kg of Dendrobium officinale and 1kg of Semen Ziziphi Spinosae, crush them with a hammer mill and pass through a 5-mesh sieve, and set aside.

[0144] 2. Add the crushed material into the multifunctional dynamic extraction tank, add 12 times the amount of water of the raw material, turn on the steam and heat to 90°C, stir and extract for 60 minutes, and then the liquid is obtained as Extract 1; for the second extraction, add 8 times the amount of water of the raw material, turn on the steam and heat to 90°C, stir and extract for 30 minutes, and then the liquid is obtained as Extract 2. Combine Extracts 1 and 2, pass through a 60-mesh sieve, and use reduced pressure concentration (-0.06Mpa~-0.08Mpa, 70±5°C) to obtain 5kg of concentrated solution with a Brix value of 11.7%. After pasteurization, the concentrated solution is poured into a sterile bag for standby use.

[0145] Comparative Example 2 Preparation of Composition B

[0146] 1. Weigh 2kg of Dendrobium officinale and 3kg of Gastrodia elata, crush them with a hammer mill and pass through a 5-mesh sieve, and set aside.

[0147] 2. Add the crushed material into the multifunctional dynamic extraction tank, add 12 times the amount of water of the raw material, turn on the steam and heat to 90°C, stir and extract for 60 minutes, and then the liquid is obtained as Extract 1; for the second extraction, add 8 times the amount of water of the raw material, turn on the steam and heat to 90°C, stir and extract for 30 minutes, and then the liquid is obtained as Extract 2. Combine Extracts 1 and 2, pass through a 60-mesh sieve, and use reduced pressure concentration (-0.06Mpa~-0.08Mpa, 70±5°C) to obtain 5kg of concentrated solution with a Brix value of 12.3%. After pasteurization, the concentrated solution is poured into a sterile bag for standby use.

[0148] Comparative Example 3 Preparation of Composition C

[0149] 1. Weigh 2kg of Dendrobium officinale and 3kg of Ophiopogon japonicus, crush them with a hammer mill and pass through a 5-mesh sieve, and set aside.

[0150] 2. Add the crushed material into the multifunctional dynamic extraction tank, add 12 times the amount of water of the raw material, turn on the steam and heat to 90°C, stir and extract for 60 minutes, and then the liquid is obtained as Extract 1; for the second extraction, add 8 times the amount of water of the raw material, turn on the steam and heat to 90°C, stir and extract for 30 minutes, and then the liquid is obtained as Extract 2. Combine Extracts 1 and 2, pass through a 60-mesh sieve, and use reduced pressure concentration (-0.06Mpa~-0.08Mpa, 70±5°C) to obtain 5kg of concentrated solution with a Brix value of 12.8%. After pasteurization, the concentrated solution is poured into a sterile bag for standby use.

[0151] Comparative Example 4 Preparation of Composition d

[0152] 1. Weigh 0.5kg of sour jujube kernel and 3kg of gastrodia elata, crush them with a hammer mill and pass through a 5-mesh sieve, and set aside.

[0153] 2. Add the crushed material into the multifunctional dynamic extraction tank, add 12 times the amount of water of the raw material, turn on the steam and heat to 90°C, stir and extract for 60 minutes, and then the liquid is obtained as Extract 1; for the second extraction, add 8 times the amount of water of the raw material, turn on the steam and heat to 90°C, stir and extract for 30 minutes, and then the liquid is obtained as Extract 2. Combine Extracts 1 and 2, pass through a 60-mesh sieve, and use reduced pressure concentration (-0.06Mpa~-0.08Mpa, 70±5°C) to obtain 3.5kg of concentrated solution with a Brix value of 12.1%. After pasteurization, the concentrated solution is poured into a sterile bag for standby use.

[0154] Comparative Example 5 Preparation of Composition e

[0155] 1. Weigh 0.5kg of Ziziphus jujuba seeds and 3kg of Radix Ophiopogonis, crush them with a hammer mill and pass through a 5-mesh sieve, and set aside.

[0156] 2. Add the crushed material into the multifunctional dynamic extraction tank, add 12 times the amount of water of the raw material, turn on the steam and heat to 90°C, stir and extract for 60 minutes, and then the liquid is obtained as Extract 1; for the second extraction, add 8 times the amount of water of the raw material, turn on the steam and heat to 90°C, stir and extract for 30 minutes, and then the liquid is obtained as Extract 2. Combine Extracts 1 and 2, pass through a 60-mesh sieve, and use reduced pressure concentration (-0.06Mpa~-0.08Mpa, 70±5°C) to obtain 3.5kg of concentrated solution with a Brix value of 12.9%. After pasteurization, the concentrated solution is poured into a sterile bag for standby use.

[0157] The formula comparison of the above embodiments and comparative examples is shown in Table 1.

[0158]

[0159] Effect Example 1

[0160] Elevated plus maze test: It uses the conflicting state between rodents' preference for dark, enclosed spaces and their fear of high, open environments to detect anxiety-like effects in animals. [Edited by Liu Xinmin, Chen Shanguang, and Qin Chuan. Animal Behavior Experimental Methodology. Beijing: China Light Industry Press, 2023]

[0161] 1. The elevated plus maze experimental apparatus includes a test box, a camera system and a computer.

[0162] The mouse elevated plus maze test box includes two 30cm×5cm relatively open arms and two 30cm×50cm×15cm relatively closed arms. The upper part of the closed arms is open, and there is a 5cm×5cm open part in the center of the maze. The maze is 60-70cm above the ground. The behavior of the animals in the maze is captured by a camera and then transmitted to the computer. The analog image is converted into a digital image, stored and analyzed to obtain the relevant test parameters. The software analysis system can automatically collect parameters such as the animal's starting position, the time required to enter the open and closed arms, search for the target, and the running trajectory, and can also perform statistics and analysis on the various data collected.

[0163] 2. Materials and Methods

[0164] 1. Experimental animals: ICR mice, male, weighing (18±2) g, provided by Shanghai Xipu-Bikai Laboratory Animal Co., Ltd., production license number: SCXK (Shanghai) 2016-008. The temperature of the breeding room is 20℃-25℃, the relative humidity is 60%-70%, and the circadian rhythm is maintained at 12h. Food and water are available freely.

[0165] 2. Animal grouping and administration: Mice were randomly divided into 12 groups, including normal control group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Example 6 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, and Comparative Example 5 group, with 8 mice in each group. The normal control group was gavaged with an equal volume of distilled water, the Example 1 group and the Example 3 group were administered at a dose of 7.75 mg / kg (equivalent to 30 times the recommended amount for the human body, 15.5 g of raw material / 60 kg for the human body), the Example 2 group and the Example 4 group were administered at a dose of 5.5 mg / kg (equivalent to 30 times the recommended amount for the human body, 11 g of raw material / 60 kg for the human body), the Example 5 group and the Example 6 group were administered at a dose of 8.4 mg / kg (equivalent to 30 times the recommended amount for the human body, 13.4 g of raw material / 60 kg for the human body), the Comparative Example 1 group was administered at a dose of 2.5 mg / kg (equivalent to 30 times the recommended amount for the human body, 5 g of raw material / 60 kg for the human body), the Comparative Example 2 group and the Comparative Example 3 group were administered at a dose of 5.0 mg / kg (equivalent to 30 times the recommended amount for the human body, 10 g of raw material / 60 kg for the human body), and the Comparative Example 4 group and the Comparative Example 5 group were administered at a dose of 3.5 mg / kg (equivalent to 30 times the recommended amount for the human body, 7 g of raw material / 60 kg for the human body). Each group was administered for 4 consecutive weeks.

[0166] 3. Stroking the mice every day for one week before the experiment to reduce the impact of irrelevant stimuli on the mice in the maze. After the last gavage on the 28th day, the mouse elevated plus maze was used for testing. The mouse was placed in the center of the maze with its head facing the open arm, and the observer was at least 1m away from the center of the maze to reduce interference. Start the software, record the number of times the mouse enters the open arm and the closed arm within 5 minutes, and calculate the number of times the mouse enters the open arm and the proportion of the stay time in the two arms (the standard for entering and exiting the arm should be strictly defined, based on all four limbs entering the arm or two paws out of the arm) to evaluate the anxiety-like behavior of the mouse.

[0167] 3. Evaluation indicators

[0168] The percentage of the number of times mice entered the open arm (OE) and the time they stayed in the open arm (OT) to the total number of times (the total number of times they entered the open arm and the closed arm) and the total time (the total time they stayed in the open arm and the closed arm) were calculated as indicators for evaluating anxiety.

[0169] 1. Percentage of arm opening times = arm opening times / total times × 100%

[0170] 2. Open arm dwell time percentage = open arm dwell time / total dwell time × 100%

[0171] SPSS 19.0 software was used for statistical analysis, and the measurement data were expressed as mean ± standard deviation. The differences between samples were compared using t-test. The test results are shown in Table 2.

[0172] Table 2 Effects of each group on the number of times mice entered the open arms and the time they stayed in the open arms ( ±SD, n=8)

[0173]

[0174] Note: Compared with the blank group, *P‹0.05, **P‹0.01; compared with the control group 2, ΔP‹0.05, ΔΔP‹0.01

[0175] The results are shown in Table 3. Compositions 1-6 prepared in Examples 1-6 of the present invention can increase the preference of mice for open arms, and the effect is significant or extremely significant. In particular, the difference between Compositions 1-6 of the present invention and Composition b obtained in Comparative Example 2 (the best effect among the comparative examples) is significant or extremely significant, indicating that the composition of the present invention has achieved unexpected technical effects.

[0176] Effect Example 2: Study on the intervention effect of Dendrobium and Gastrodia Drink (Example 7) and Dendrobium and Ophiopogon Drink (Example 8) on anxiety and sleep level of college students

[0177] Table 3 Hamilton Anxiety Rating Scale (HAMA)

[0178]

[0180] The anxiety level is divided according to the scoring criteria of the HAMA scale. If the total score is ≥29 points, it may be severe anxiety; ≥21 points, there is definitely obvious anxiety; ≥14 points, there is definitely anxiety; ≥7 points, there may be anxiety; <7 points, there is no anxiety symptom.

[0181] 90 students who definitely had anxiety (≥7 points) were randomly selected and divided into 3 groups, each with 30 students. They were respectively intervened with mineral water, Shihu Tianma Drink (Example 7) and Shihu Maidong Drink (Example 8), 1 bottle per day, for 4 consecutive weeks. The HAMA scale scores of the students before and after the intervention were statistically processed, and the t-test was used for the intra-group and inter-group levels. The results are shown in Table 4.

[0182] Table 4 Statistical difference analysis of HAMA scale scores of students before and after intervention ( ±SD, n=26)

[0183]

[0184] Note: Compared with before intervention, **P ‹ 0.01, compared with the control group, ΔΔP ‹ 0.01

[0185] The results are shown in Table 4. The anxiety level of the control group did not change significantly before and after the intervention. The average score of the HAMA scale after consuming Shihu Tianma Drink (Example 7) was 5.54 points, which was significantly lower than the average score of 9.58 points before the intervention. The difference in anxiety level before and after the intervention reached an extremely significant level, and compared with the control group, the difference was also extremely significant. The average score of the HAMA scale after consuming Shihu Maidong Drink (Example 8) was 5.62 points, which was significantly lower than the average score of 10.04 points before the intervention. The difference in anxiety level before and after the intervention reached an extremely significant level, and compared with the control group, the difference was also extremely significant. It shows that consuming Shihu Tianma Drink (Example 7) and Shihu Maidong Drink (Example 8) has a certain intervention effect on alleviating anxiety and insomnia in college students.

[0186] Effect Example 3

[0187] 1. Testing basis: "Health Food Function Test and Evaluation Method (2023 Edition)"

[0188] 2. Materials and Methods

[0189] 1. Experimental animals: ICR mice, male, weighing (18±2) g, provided by Shanghai Xipu-Bikai Laboratory Animal Co., Ltd., production license number: SCXK (Shanghai) 2016-008. The temperature of the breeding room was 20℃-25℃, the relative humidity was 60%-70%, and a 12-hour circadian rhythm was maintained, with free access to food and water.

[0190] 2. Animal grouping and administration: Mice were randomly divided into 12 groups, including normal control group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Example 6 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, and Comparative Example 5 group, with 8 mice in each group. The normal control group was gavaged with an equal volume of distilled water, the Example 1 group and the Example 3 group were administered at a dose of 7.75 mg / kg (equivalent to 30 times the recommended amount for the human body, 15.5 g of raw material / 60 kg recommended for the human body), the Example 2 group and the Example 4 group were administered at a dose of 5.5 mg / kg (equivalent to 30 times the recommended amount for the human body, 11 g of raw material / 60 kg recommended for the human body), the Example 5 group and the Example 6 group were administered at a dose of 8.4 mg / kg (equivalent to 30 times the recommended amount for the human body, 13.4 g of raw material / 60 kg recommended for the human body), the Comparative Example 1 group was administered at a dose of 2.5 mg / kg (equivalent to 30 times the recommended amount for the human body, 5 g of raw material / 60 kg recommended for the human body), the Comparative Example 2 group and the Comparative Example 3 group were administered at a dose of 5.0 mg / kg (equivalent to 30 times the recommended amount for the human body, 10 g of raw material / 60 kg recommended for the human body), and the Comparative Example 4 group and the Comparative Example 5 group were administered at a dose of 3.5 mg / kg (equivalent to 30 times the recommended amount for the human body, 7 g of raw material / 60 kg recommended for the human body). After each group was given the drug for 4 weeks, ConA-induced mouse spleen lymphocyte transformation experiment and NK cell activity experiment were performed.

[0191] 3. ConA-induced mouse spleen lymphocyte transformation experiment: MTT method. According to the "Test Method for Immunity Enhancement" of "Health Food Function Test and Evaluation Method (2023 Edition)".

[0192] 4. NK cell activity test: lactate dehydrogenase (LDH) assay. Conducted in accordance with the “Test Methods for Immunity Enhancement” in the “Test and Evaluation Methods for Health Food Functions (2023 Edition)”.

[0193] 5. Data processing and result determination

[0194] The test was conducted according to the "Test Method for Immunity Enhancement" in the "Test and Evaluation Method for Health Food Function (2023 Edition)". SPSS19.0 software was used for statistical processing, and the measurement data were expressed as mean ± standard deviation, and the difference between samples was compared using t test. The test results are shown in Tables 5 and 6.

[0195] Table 5 ConA-induced mouse spleen lymphocyte proliferation test ( ±SD, n=8)

[0196]

[0197] Note: Compared with the blank group, *P‹0.05, **P‹0.01; compared with the control group 3, ΔP‹0.05, ΔΔP‹0.01

[0198] Table 6 NK cell activity of mice in each group ( ±SD, n=8)

[0199]

[0200] Note: Compared with the blank group, *P‹0.05, **P‹0.01; compared with the control group 3, ΔP‹0.05, ΔΔP‹0.01

[0201] As shown in Table 5 and Table 6, the compositions 1-6 prepared in Examples 1-6 of the present invention and the compositions ae of Comparative Examples 1-5 all have the effect of helping to enhance immunity. However, the difference between the compositions 1-6 of the present invention and the composition c obtained in Comparative Example 3 (the best effect among the comparative examples) is significant or extremely significant, indicating that the compositions of the present invention have achieved unexpected technical effects.

Claims

1. A Chinese medicine composition, comprising the following Chinese medicines in parts by weight: 1-3 parts of Dendrobium officinale, 0.2-0.3 parts of Ziziphus jujuba seeds, 1-3 parts of Gastrodia elata; The Gastrodia elata can be replaced by Ophiopogon japonicus, with 1-3 parts of Ophiopogon japonicus being used in parts by weight.

2. The Chinese medicine composition according to claim 1, characterized in that The Chinese medicine comprises the following Chinese medicines by weight: 1-3 parts of Dendrobium officinale, 0.2-0.3 parts of Ziziphus jujuba seeds, 1-3 parts of Gastrodia elata, 0.8-1.6 parts of Polygonatum odoratum, 0.6-1 parts of Honeysuckle, 0.5-0.9 parts of Cistanche deserticola, and 0.2-0.3 parts of Chrysanthemum; the Gastrodia elata can be replaced by Ophiopogon japonicus, and by weight, the Ophiopogon japonicus is 1-3 parts.

3. The Chinese medicine composition according to claim 1, characterized in that: It meets one of the following conditions: (1) In parts by weight, the Chinese medicine composition is composed of 1-3 parts of Dendrobium officinale, 0.2-0.3 parts of Semen Ziziphi Spinosae and 1-3 parts of Gastrodia elata; (2) In terms of weight, the Chinese medicine composition is composed of 1-3 parts of Dendrobium officinale, 0.2-0.3 parts of Semen Ziziphi Spinosae and 1-3 parts of Radix Ophiopogonis; (3) In parts by weight, the Chinese medicine composition is composed of 1-3 parts of Dendrobium officinale, 0.2-0.3 parts of Ziziphus jujuba seeds, 1-3 parts of Gastrodia elata, 0.8-1.6 parts of Polygonatum odoratum, 0.6-1 parts of Honeysuckle, 0.5-0.9 parts of Cistanche deserticola, and 0.2-0.3 parts of Chrysanthemum; the Gastrodia elata can be replaced by Ophiopogon japonicus, in which case the Ophiopogon japonicus is 1-3 parts in parts by weight.

4. The Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is any of the following combinations: Combination 1: In parts by weight, the Chinese medicine composition consists of 2 parts of Dendrobium officinale, 0.25 parts of Semen Ziziphi Spinosae and 1.5 parts of Gastrodia elata; Combination 2: In terms of weight, the Chinese medicine composition is composed of 2 parts of Dendrobium officinale, 0.25 parts of Semen Ziziphi Spinosae and 1.5 parts of Radix Ophiopogonis; Combination 3: In parts by weight, the Chinese medicine composition is composed of 2 parts of Dendrobium officinale, 0.25 parts of Ziziphus jujuba seeds, 1.5 parts of Gastrodia elata, 1.2 parts of Polygonatum odoratum, 0.8 parts of Honeysuckle, 0.7 parts of Cistanche deserticola, and 0.25 parts of Chrysanthemum; Combination 4: In parts by weight, the Chinese medicine composition consists of 2 parts of Dendrobium officinale, 0.25 parts of Ziziphus jujuba seeds, 1.5 parts of Ophiopogon japonicus, 1.2 parts of Polygonatum odoratum, 0.8 parts of Honeysuckle, 0.7 parts of Cistanche deserticola, and 0.25 parts of Chrysanthemum.

5. A medicinal and edible composition, comprising a Dendrobium officinale extract, a Ziziphus jujuba seed extract, a Gastrodia elata extract or an Ophiopogon japonicus extract, wherein the weight proportions of the Dendrobium officinale used in the Dendrobium officinale extract, the Ziziphus jujuba seed used in the Ziziphus jujuba seed extract, the Gastrodia elata used in the Gastrodia elata extract and the Ophiopogon japonicus used in the Ophiopogon japonicus extract are the same as those described in any one of claims 1 to 4.

6. The medicine-food composition according to claim 5, characterized in that: It meets at least one of the following conditions: (1) It also includes polygonatum odoratum extract, honeysuckle extract, cistanche extract, and chrysanthemum extract, and the weight proportions of polygonatum used in the polygonatum odoratum extract, honeysuckle used in the honeysuckle extract, cistanche used in the cistanche extract, and chrysanthemum used in the chrysanthemum extract are the same as those in any one of claims 2 to 4; (2) It also includes a sweetener and / or a sour agent; (3) It also includes one or more of a preservative, an excipient and an antioxidant.

7. The edible-medicinal composition according to any one of claims 5 to 6, characterized in that: It meets one or more of the following conditions: (1) The Dendrobium officinale extract is a Dendrobium officinale extract obtained by water extraction; (2) The Chinese jujube seed extract is a Chinese jujube seed extract obtained by water extraction; (3) The Gastrodia elata extract is obtained by water extraction; (4) The Ophiopogon japonicus extract is an Ophiopogon japonicus extract obtained by water extraction; (5) The polygonatum odoratum extract is obtained by water extraction; (6) The honeysuckle extract is obtained by water extraction; (7) The Cistanche deserticola extract is obtained by water extraction; (8) The chrysanthemum extract is a chrysanthemum extract obtained by water extraction; (9) The sweetener is one or more of white sugar, glucose, sugar alcohols, sucralose, aspartame, acesulfame potassium, and stevioside, such as white sugar; (10) The acidulant is one or more of citric acid, malic acid, and tartaric acid, such as citric acid; (11) The antioxidant is one or more of L-ascorbic acid (vitamin C), sodium L-ascorbate, isoascorbic acid, sodium isoascorbate, tea polyphenols, vitamin E, and superoxide dismutase, such as L-ascorbic acid (vitamin C); (12) The preservative is one or more of sorbic acid, potassium sorbate, parabens, chitosan, and tea polyphenols, such as sorbic acid; (13) The excipient is one or more of maltodextrin, mannitol, lactose, soluble starch, and β-cyclodextrin, such as maltodextrin; (14) In the edible-medicinal composition, the ratio of the extract, water, the sweetener and the acidulant is 54:(25-50):(4-8):(0.01-0.09) by weight, for example, 54:39.95:6:0.

05.

8. The medicine-food composition according to claim 6, characterized in that: It meets one of the following conditions: (1) The edible and medicinal composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, and the Gastrodia elata extract; preferably, the Brix value of the edible and medicinal composition is 10%-25%, for example, 12.6%, 13.5%; (2) The edible and medicinal composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, the Gastrodia elata extract, the Polygonatum odoratum extract, the Lonicera japonica extract, the Cistanche deserticola extract, and the Chrysanthemum extract; preferably, the Brix value of the edible and medicinal composition is 10%-25%, for example, 11.9%; (3) The medicinal and edible composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, the Gastrodia elata extract, the Polygonatum odoratum extract, the Honeysuckle extract, the Cistanche deserticola extract, the Chrysanthemum extract, white sugar, and citric acid; (4) The edible and medicinal composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, and the Ophiopogon japonicus extract; preferably, the Brix value of the edible and medicinal composition is 10%-25%, for example, 13.1%, 13.9%; (5) The edible and medicinal composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, the Ophiopogon japonicus extract, the Polygonatum odoratum extract, the Lonicera japonica extract, the Cistanche deserticola extract, and the Chrysanthemum flower extract; preferably, the Brix value of the edible and medicinal composition is 10%-25%, for example, 12.2%; (6) The medicine-food composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, the Ophiopogon japonicus extract, the Polygonatum odoratum extract, the Honeysuckle extract, the Cistanche deserticola extract, the Chrysanthemum extract, white sugar, and citric acid; (7) The medicine-food composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, the Gastrodia elata extract, the Polygonatum odoratum extract, the Lonicera japonica extract, the Cistanche deserticola extract, the Chrysanthemum extract, white sugar, citric acid, the preservative and the antioxidant; (8) The medicine-food composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, the Ophiopogon japonicus extract, the Polygonatum odoratum extract, the Honeysuckle extract, the Cistanche deserticola extract, the Chrysanthemum extract, white sugar, citric acid, the preservative and the antioxidant; (9) The medicine-food composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, the Gastrodia elata extract, the Polygonatum odoratum extract, the Honeysuckle extract, the Cistanche deserticola extract, the Chrysanthemum extract, white sugar, citric acid, the preservative, the excipient and the antioxidant; (10) The medicine-food composition is composed of the Dendrobium officinale extract, the Ziziphus jujuba seed extract, the Ophiopogon japonicus extract, the Polygonatum odoratum extract, the Lonicera japonica extract, the Cistanche deserticola extract, the Chrysanthemum extract, white sugar, citric acid, the preservative, the excipient and the antioxidant.

9. The medicine-food composition according to claim 5, characterized in that: It comprises the following steps to prepare: S1: heating the Chinese medicine composition according to any one of claims 1 to 4 with water, and filtering to obtain an extract 1 and a filter residue; S2: heating the filter residue and water, filtering to obtain extract 2; combining the extract 1 and the extract 2, filtering to obtain a mixed extract; S3: centrifuging and filtering the mixed extract, concentrating and sterilizing the intercepted liquid obtained by filtration to obtain a concentrated liquid, thereby obtaining the medicinal and edible composition; Preferably, it satisfies one or more of the following conditions: (1) In S1, the mass ratio of the water to the Chinese medicine composition is 12-15:1; (2) In S1, the heating temperature is 90±5°C; (3) In S1, the heating time is 120-90 minutes; (4) In S2, the mass ratio of the water to the filter residue is 8-10:1; (5) In S2, the heating temperature is 90±5°C; (6) In S2, the heating time is 60-30 minutes; (7) In S2, the filtering is sieving, and the sieving screen is 40-80 mesh, for example, 60 mesh; (8) In S3, the centrifugation is performed using a tubular centrifuge; (9) In S3, the filtration is to filter the centrifuge liquid obtained by the centrifugation using a ceramic membrane; the pore size of the ceramic membrane filtration is 0.2 μm; the permeate flow rate of the ceramic membrane filtration is 60 L / hr; (10) In S3, the concentration is performed by using a reverse osmosis membrane; the molecular weight cutoff of the reverse osmosis membrane concentration is 100; and the permeate flow rate of the reverse osmosis membrane concentration is 90 L / hr; (11) In S3, the Brix value of the concentrate is 10%-25%, for example, 11.9%; (12) In S3, the sterilization is pasteurization; (13) The method for preparing the edible-medicinal composition further comprises step S4: heating the concentrated solution and water for the first time, and then heating the concentrated solution with a sweetener, an acidulant, an antioxidant and a preservative for the second time to obtain the edible-medicinal composition; More preferably, it satisfies one or more of the following conditions: (1) In S4, the mass ratio of the concentrate to the water is 8:3, for example, 16.08:6; (2) In S4, the temperature of the first heating is 80° C.; (3) In S4, the sweetener is white sugar; (4) In S4, the mass ratio of the concentrate to the sweetener is 16.08:1.8; (5) In S4, the acidulant is citric acid; (6) In S4, the mass ratio of the concentrate to the acidulant is 16.08:0.0015; (7) In S4, the temperature of the second heating is 95±5°C; (8) In S4, the second heating time is 10-20 minutes, for example, 15 minutes; (9) In S4, water is added after the second heating; the mass ratio of the added water to the concentrated liquid is 30:16.

08.

10. Use of the Chinese medicine composition according to any one of claims 1 to 4 or the medicine-food composition according to any one of claims 5 to 9 in the preparation of a medicament for treating one or more of antianxiety, improving sleep and enhancing immunity.

Images