Composition with repairing and soothing effects as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a composition with repairing and soothing effects and a preparation method and application thereof. The soluble collagen, the centella asiatica extract and the hydrolyzed collagen are used in a matched mode, the limitation of a single component can be effectively overcome through mutual cooperation of the soluble collagen, the centella asiatica extract and the hydrolyzed collagen, expression of cell fibronectin (FLG) and aquaporin 3 (AQP3) is remarkably promoted, activity of kallikrein related peptidase 7 (KLK7) is inhibited, release of IL-6 and IL-8 in HaCaT cells is reduced, and the anti-inflammatory effect is achieved. In addition, the A-type humanized collagen and the natural plant extract are adopted, the components are mild, and the skin care product is suitable for being used on sensitive skin.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to a composition with repairing and soothing effects, and a preparation method and application thereof. Background Art

[0002] With the intensification of environmental pollution, increased ultraviolet radiation and increased life pressure, the incidence of skin barrier damage, sensitivity and fragility has increased significantly. According to the "Chinese Expert Consensus on the Diagnosis and Treatment of Sensitive Skin", the incidence of sensitive skin in Chinese women has reached 36.1%. Damage to the skin barrier function not only leads to immediate symptoms such as dryness, redness and itching, but also triggers chronic inflammatory reactions and accelerates the aging process of the skin.

[0003] Currently, most repair products on the market use a single active ingredient system: collagen products mainly rely on the physical film-forming effect of large-molecule collagen, but have a low transdermal absorption rate (<8%) and cannot activate extracellular matrix regeneration; although Centella asiatica extract has anti-inflammatory properties, its triterpenoid components (such as asiatica glycoside) are easily affected by the polyol solvent system in conventional formulas, resulting in a 30%-40% decrease in bioavailability; and because the molecular weight of hydrolyzed gel is too small (<5kDa), although it can penetrate the stratum corneum when used alone, it lacks long-term moisturizing ability.

[0004] On the other hand, most products on the market rely on phenoxyethanol and paraben preservative systems. These ingredients have been proven to disrupt the balance of skin flora (see a 2021 study in the Journal of Investigative Dermatology) and aggravate the immune stress response of sensitive skin.

[0005] In this context, developing a composition that has multiple barrier repair mechanisms (physical barrier + immune barrier + microbial barrier), breaks through the technical bottlenecks of transdermal absorption and activity retention through synergistic enhancement of ingredients, and does not contain irritating preservatives has become a technical problem that needs to be urgently solved in the field of skin repair. Summary of the invention

[0006] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide a composition with repairing and soothing effects, which effectively improves the skin barrier function, reduces inflammatory response, and promotes skin moisture retention through the synergistic effect of multiple active ingredients.

[0007] To achieve the above objectives, the technical solutions adopted by the present invention include:

[0008] In a first aspect, the present invention provides a composition with repairing and soothing effects, comprising the following components in percentage by mass: 0.01-0.1% soluble collagen, 0.1-0.8% Centella asiatica extract, 0.005-0.08% hydrolyzed collagen, 0.45-2.6% auxiliary ingredients, 25.5-37% moisturizer and the remainder water.

[0009] The present invention uses soluble collagen, Centella asiatica extract and hydrolyzed collagen in combination. The three work together to effectively overcome the limitations of a single ingredient and synergistically enhance the overall skin repair effect. Soluble collagen protects the skin through its physical film-forming effect, Centella asiatica extract plays a key role in repairing skin inflammation and improving the skin barrier, and hydrolyzed collagen promotes penetration and provides immediate hydration through its smaller molecular weight; the synergistic effect of the three can not only effectively enhance the barrier function of the skin, reduce inflammatory response, but also promote the lasting retention of skin moisture. The composition of the present invention is suitable for the repair and soothing of sensitive skin, can improve the skin's tolerance and enhance the skin's self-repair ability, and further enhance the product's bioavailability and effect. Through this innovative formula, the present invention provides a more comprehensive, long-lasting, gentle and safe skin repair solution that can improve skin condition in the short term and provide continuous support for long-term skin health.

[0010] In addition, the inventors explored the dosage of each component and found that when the dosage ratio of the three active ingredients, soluble collagen, Centella asiatica extract and hydrolyzed collagen, is within the above-mentioned preferred range, the components cooperate with each other to exert an ideal synergistic effect, thereby effectively improving the repair and soothing effects of the composition with good cost performance. However, when the dosage ratio of the three exceeds the specified range, the synergistic effect between the components is not obvious, which in turn leads to a decrease in the repair and soothing effects of the composition.

[0011] Preferably, the soluble collagen and hydrolyzed collagen are both type A recombinant humanized collagen.

[0012] The soluble collagen and hydrolyzed collagen used in the present invention are both type A recombinant humanized collagen. Type A recombinant humanized collagen has properties that are highly similar to natural human collagen, and can better promote skin repair, improve biocompatibility and provide better skin care effects, especially in skin repair and soothing applications.

[0013] Preferably, the composition comprises the following components in percentage by mass: 0.02-0.08% soluble collagen, 0.1-0.5% Centella asiatica extract, 0.005-0.05% hydrolyzed collagen, 0.75-1.58% auxiliary ingredients, 29-33.5% moisturizer and the balance water.

[0014] Preferably, the composition comprises the following components in percentage by mass: 0.04% soluble collagen, 0.2% Centella asiatica extract, 0.01% hydrolyzed collagen, 1.1% auxiliary ingredients, 31.2% moisturizing agent and the balance water.

[0015] Through experimental research, it was found that when the components of the present invention are combined using the optimal dosage ratio specified above, the prepared composition can effectively promote the expression of cell filaggrin (FLG) and aquaporin 3 (AQP3), and can also inhibit the activity of kallikrein-related peptidase 7 (KLK7), reduce the release of inflammatory factors IL-6 and IL-8, and achieve the best repair and soothing effect.

[0016] Preferably, the auxiliary ingredients include mannitol, caprylyl glycol and ethylhexylglycerin, and the mass ratio of mannitol, caprylyl glycol and ethylhexylglycerin is (0.3-1.5):(0.1-1):(0.05-0.1).

[0017] As a sugar alcohol compound, mannitol helps to enhance the hydration of the skin, thereby improving the transdermal properties of the active ingredients in the composition, and effectively helping the active ingredients to penetrate the skin; caprylyl glycol can reduce the tightness of the skin barrier, thereby enhancing the penetration effect of the active ingredients; ethylhexylglycerol can help other active ingredients to penetrate better by dissolving in the lipid layer in the skin, and play a certain role in promoting penetration. Experimental research has found that the present invention uses the above three components as auxiliary ingredients. The three cooperate with each other within the above preferred amount ratio range, which can synergistically enhance the skin penetration effect of the three active ingredients of soluble collagen, Centella asiatica extract and hydrolyzed collagen, effectively enhance the bioavailability of the active ingredients, and further enhance the skin repair and soothing effect of the composition.

[0018] Preferably, the mass ratio of mannitol, caprylyl glycol and ethylhexylglycerin is (0.5-1):(0.2-0.5):(0.05-0.08).

[0019] More preferably, the mass ratio of mannitol, caprylyl glycol and ethylhexylglycerin is 0.8:0.225:0.075.

[0020] Preferably, the moisturizing agent comprises 1,3-propylene glycol and butylene glycol, and the mass ratio of the 1,3-propylene glycol to butylene glycol is (0.5-2):(25-35).

[0021] Preferably, the mass ratio of 1,3-propylene glycol to butanediol is (1-1.5):(28-32).

[0022] More preferably, the mass ratio of 1,3-propylene glycol to butanediol is 1.2:30.

[0023] In a second aspect, the present invention provides use of the composition in preparing cosmetics.

[0024] In a third aspect, the present invention provides a method for preparing the composition, comprising the following steps:

[0025] All components are mixed evenly and stirred until they are completely dissolved to obtain the composition.

[0026] In a fourth aspect, the present invention provides a cosmetic having a repairing and soothing effect, wherein the cosmetic comprises the composition.

[0027] Preferably, the mass percentage of the composition in the cosmetic is 1-10%.

[0028] More preferably, the mass percentage of the composition in the cosmetic is 5%.

[0029] Preferably, the cosmetics include facial creams, essences, facial masks and lotions.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] The present invention uses soluble collagen, Centella asiatica extract and hydrolyzed collagen in combination. The cooperation of the three can effectively overcome the limitations of a single ingredient, significantly promote the expression of filaggrin (FLG) and aquaporin 3 (AQP3), inhibit the activity of kallikrein-related peptidase 7 (KLK7), and reduce the release of IL-6 and IL-8 in HaCaT cells, thereby synergistically enhancing the skin barrier repair and soothing effects. In addition, it uses type A humanized collagen and natural plant extracts, and the ingredients are mild, making it suitable for sensitive skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 This is a graph showing the test results of the skin barrier repair efficacy at the cellular level of the composition (CO-Jifuyin) prepared in Example 1;

[0033] Figure 2 This is a graph showing the results of the anti-inflammatory and soothing activity test of the composition (CO-Jifuyin) prepared in Example 1 at the cellular level;

[0034] Figure 3 This is the appearance of the finished product of the composition (CO-Jifuyin) prepared in Example 1. DETAILED DESCRIPTION

[0035] To better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. It should be understood by those skilled in the art that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0036] Unless otherwise specified, all raw materials involved in the following examples and comparative examples are commercially available general materials.

[0037] The soluble collagen and hydrolyzed collagen described in the examples and comparative examples are both type A recombinant humanized collagen.

[0038] Examples 1-7

[0039] This embodiment 1-7 provides a composition with repairing and soothing effects, and the specific formula is shown in Table 1. The preparation method of the composition comprises the following steps:

[0040] All ingredients are mixed evenly and stirred until completely dissolved to obtain the composition.

[0041] Table 1 Formula of the composition described in Examples 1-7 (mass percentage)

[0042]

[0043]

[0044] Comparative Examples 1-9

[0045] Comparative Examples 1-9 provide a composition with a repairing and soothing effect, and the specific formula is shown in Table 2. The preparation method of the composition comprises the following steps:

[0046] All ingredients are mixed evenly and stirred until completely dissolved to obtain the composition.

[0047] Table 2 Formula of the composition described in Comparative Examples 1-9 (mass percentage)

[0048]

[0049]

[0050] Comparative Example 10

[0051] This comparative example provides a composition with repairing and soothing effects, which is different from Example 1 only in that an equal amount of Portulaca oleracea extract is used to replace the Centella asiatica extract, and the other components and amounts remain unchanged.

[0052] Effect example

[0053] 1. Cellular level skin barrier repair efficacy test

[0054] The specific method is as follows:

[0055] (1) Cell inoculation: Take HaCaT cells in the logarithmic growth phase and dilute the cell number to 4×10 5100 cells / well, add 2 mL of cell suspension to the 6-well plate, and observe under a microscope whether the cell density in the 6-well plate is uniform. Place the cells in a cell culture incubator and culture for 24 hours.

[0056] (2) Experimental groups: a blank control group, a negative control group, a positive control group (10 μM WY-14643) and a sample group, each group having 3 replicates; the sample group is the composition prepared in Examples 1-7 and Comparative Examples 1-10.

[0057] (3) Sample preparation: The compositions of the sample groups were mixed with water to prepare test sample solutions with a concentration of 5%, wherein the composition of Example 1 was prepared into test sample solutions with concentrations of 1% and 5%, respectively.

[0058] (4) SDS treatment and sample incubation: When the cells are confluent to 70-80%, remove the culture medium, carefully rinse the cell growth surface once with PBS, add SDS to the negative control group, sample group, and positive control group, add 2 mL DMEM (without serum) to the blank group, and continue incubating in the incubator for 24 hours. Remove the culture medium, carefully rinse the cell growth surface once with PBS, add 2 mL DMEM (without serum) to the blank group and negative control group; add 2 mL DMEM (without serum) to the sample group; add 2 mL DMEM (without serum) containing the sample to be tested to the positive control group; add 2 mL DMEM (without serum) containing 10 μM WY14643 to the positive control group, and continue incubating for 24 hours.

[0059] (5) RNA extraction: RNA extraction was performed according to the instructions in the RNAisolater Total RNA Extraction Reagent Kit. The qPCR primer sequences involved are shown in Table 3.

[0060] (6) qRT-PCR: refer to the instructions in the HiScript II One Step qRT-PCR SYBR Green Kit. -△△Ct The relative expression of mRNA of each index was calculated according to the calculation rules. The specific results are shown in Table 4.

[0061] Table 3 qPCR primer sequences

[0062]

[0063] Table 4 Results of barrier repair activity at the cellular level

[0064]

[0065]

[0066] The results in Table 4 show that the composition described in the examples of the present invention can significantly upregulate the transcription of filaggrin (FLG) and aquaporin 3 (AQP3) at the RNA level at a concentration of 5%, and significantly downregulate kallikrein 7 (KLK7), thereby effectively promoting the differentiation of keratinocytes, promoting the transport of water and glycerol to the epidermis, inhibiting the abnormal shedding of the stratum corneum, and effectively promoting the repair of the skin barrier. In particular, the barrier repair activity of the composition described in Example 1 is the best. When one or two of the three active ingredients of soluble collagen, Centella asiatica extract and hydrolyzed collagen are missing in Comparative Examples 1-5, compared with the examples, the effect of the prepared composition on up-regulating the transcription of FLG and AQP3 is significantly reduced, and the effect of down-regulating the transcription of KLK7 is also significantly worse, that is, the barrier repair effect on the skin is significantly worse, especially when the soluble collagen component is missing, the repair effect is most significantly reduced, and when the amount ratio of the above three components in Comparative Examples 6-7 exceeds the preferred range of the present invention, the skin repair effect of the composition is also significantly worse than that of the examples. It can be seen that when the three active ingredients of soluble collagen, Centella asiatica extract and hydrolyzed collagen are matched within a specific dosage range, the skin barrier repair effect of the composition can be better improved, and the synergistic effect between the components is significant. When the amount ratio of the three active ingredients exceeds the preferred range, the synergistic effect between the components is not obvious or even produces antagonism, resulting in the barrier repair effect of the composition still decreasing compared with the examples when the soluble collagen is used in a large amount.

[0067] 2. Anti-inflammatory and soothing activity test

[0068] The specific method is as follows:

[0069] (1) Cell inoculation: HaCaT cells in logarithmic growth phase were plated at 4×10 4 The cells were seeded at a density of 1.54 × 10 / well in a 6-well plate and incubated in a CO2 incubator for 24 h.

[0070] (2) Experimental groups: A blank control group, a negative control group, a sample group, and a positive control group (30 μg / mL dexamethasone) were set up, with 3 replicates in each group.

[0071] (3) Sample preparation: The compositions of the sample groups were mixed with water to prepare test sample solutions with a concentration of 5%, wherein the composition of Example 1 was prepared into test sample solutions with concentrations of 1% and 5%, respectively.

[0072] (4) Sample incubation and LPS stimulation: Discard the culture medium in each well of the plate, add DMEM basal medium containing a certain concentration of the test substance to the test substance wells and the positive control group, and add 2 mL of serum-free DMEM basal medium to the negative control and blank control groups, with 2 mL added to each well. After incubation in the incubator for 6 h, add an equal amount of LPS to the negative control group, positive control group, and sample group to a final concentration of 10 μg / mL and continue incubation for 18 h.

[0073] (5) RNA extraction: RNA extraction was performed according to the instructions in the RNA isolater Total RNA Extraction Reagent kit. The qPCR primer sequences involved are shown in Table 3.

[0074] (6) qRT-PCR: refer to the instructions in the HiScript II One Step qRT-PCR SYBR Green Kit. -△△Ct The relative expression of mRNA of each index was calculated according to the calculation rules. The specific results are shown in Table 5.

[0075] Table 5 Anti-inflammatory and soothing activity results

[0076]

[0077]

[0078] The results in Table 5 show that the composition prepared in the embodiment of the present invention can significantly downregulate the transcription of IL-6 and IL-8 in LPS-induced HaCaT cells at the RNA level at a concentration of 5%, thereby effectively improving capillary permeability and inhibiting vasodilation by inhibiting the expression of inflammatory factors, thereby playing an anti-inflammatory and soothing role. When any one or two of soluble collagen, Centella asiatica extract and hydrolyzed collagen are missing in Comparative Examples 1-5, the prepared composition has a significantly reduced effect on down-regulating IL-6 and IL-8 transcription, that is, the soothing and anti-inflammatory effects are significantly reduced. When the dosage of the above three active components in Comparative Examples 6-7 exceeds the specified range of the present invention, the anti-inflammatory and soothing effects of the prepared composition are also significantly deteriorated, indicating that when the three active ingredients of soluble collagen, Centella asiatica extract and hydrolyzed collagen are compounded within a specific dosage range, the synergistic effect can be better exerted to effectively improve the soothing effect of the composition, and none of the three is dispensable. When the Portulaca oleracea extract, which also has anti-inflammatory effects, is used to replace the Centella asiatica extract in Comparative Example 10, the anti-inflammatory and soothing effects of the prepared composition are still significantly reduced compared with those in the examples, further proving that the Centella asiatica extract selected in the present invention interacts with the soluble collagen and the hydrolyzed collagen and influences each other to produce a more ideal synergistic anti-inflammatory effect, and the components cannot be simply replaced. When one or two of the auxiliary ingredients of mannitol, caprylyl glycol and ethylhexylglycerin are missing in Comparative Examples 8-9, the effect of the composition on downregulating IL-6 and IL-8 inflammatory factors also decreases to a certain extent, indicating that the present invention uses the above three components as auxiliary ingredients to further enhance the penetration effect of the active ingredients and enhance the hydration of the skin to a certain extent, thereby enhancing the bioavailability of the active ingredients and improving the soothing and anti-inflammatory effects of the composition.

[0079] Finally, it should be noted that the above embodiments are intended to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A composition with repairing and soothing effects, characterized in that: The invention comprises the following components in percentage by mass: 0.01-0.1% of soluble collagen, 0.1-0.8% of Centella asiatica extract, 0.005-0.08% of hydrolyzed collagen, 0.45-2.6% of auxiliary components, 25.5-37% of moisturizing agent and the balance of water.

2. The composition according to claim 1, characterized in that The invention comprises the following components in percentage by mass: 0.02-0.08% of soluble collagen, 0.1-0.5% of Centella asiatica extract, 0.005-0.05% of hydrolyzed collagen, 0.75-1.58% of auxiliary components, 29-33.5% of moisturizing agent and the balance of water.

3. The composition according to claim 2, characterized in that The invention comprises the following components in percentage by mass: 0.04% of soluble collagen, 0.2% of Centella asiatica extract, 0.01% of hydrolyzed collagen, 1.1% of auxiliary components, 31.2% of moisturizing agent and the balance of water.

4. The composition according to any one of claims 1 to 3, characterized in that The auxiliary components include mannitol, caprylyl glycol and ethylhexylglycerin, and the mass ratio of mannitol, caprylyl glycol and ethylhexylglycerin is (0.3-1.5): (0.1-1): (0.05-0.1).

5. The composition according to any one of claims 1 to 3, characterized in that The moisturizing agent comprises 1,3-propylene glycol and butylene glycol, and the mass ratio of the 1,3-propylene glycol to butylene glycol is (0.5-2):(25-35).

6. The composition according to any one of claims 1 to 3, characterized in that The soluble collagen and hydrolyzed collagen are both type A recombinant humanized collagen.

7. Use of the composition according to any one of claims 1 to 6 in the preparation of cosmetics.

8. The method for preparing the composition according to any one of claims 1 to 6, characterized in that: The following steps are involved: All components are mixed evenly and stirred until they are completely dissolved to obtain the composition.

9. A cosmetic with repairing and soothing effects, characterized in that: The cosmetic comprises the composition according to any one of claims 1 to 6.

10. The cosmetic according to claim 9, characterized in that The mass percentage of the composition in cosmetics is 1-10%.

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