Traditional Chinese medicine composition for treating nephrotic syndrome

Abstract

The invention discloses a traditional Chinese medicine composition for treating nephrotic syndrome, and belongs to the technical field of nephrotic syndrome treatment. The traditional Chinese medicine composition disclosed by the invention can effectively treat the nephrotic syndrome, the pathological mechanism of the nephrotic syndrome can be intervened from a plurality of links through reasonable compatibility and synergistic effect of various traditional Chinese medicines, and the astragalus membranaceus can enhance the resistance of a patient, prevent infection, induce diuresis to alleviate edema and improve kidney blood circulation, so that the nephrotic syndrome can be effectively treated. The kidney function recovery is promoted; chinese yam can regulate disordered immune functions of patients, reduce proteinuria and relieve kidney protein loss. According to the traditional Chinese medicine composition for treating nephrotic syndrome, various medicines act together to comprehensively improve the pathological state of the kidney and achieve the purpose of treating nephrotic syndrome, the mantis egg-case, the fructus rosae laevigatae, the semen euryales and other traditional Chinese medicines have the effects of securing essence and reducing urination, and can effectively reduce the loss of protein and other fine substances in the kidney, so that the proteinuria level is reduced. The content of creatinine in blood is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of nephrotic syndrome treatment, and in particular relates to a traditional Chinese medicine composition for treating nephrotic syndrome. Background Art

[0002] Nephrotic syndrome is a relatively complex kidney disease that poses a serious threat to the health of patients. Its main characteristics are massive proteinuria, hypoproteinemia, edema, and hyperlipidemia. The presence of massive proteinuria means that the filtration function of the kidney is seriously disturbed and a large amount of protein is lost from the urine. This will not only cause a sharp drop in the protein level in the patient's body, leading to hypoproteinemia, but will also further affect the normal functions of various organs and systems in the body. Hypoproteinemia can cause a series of problems in patients, such as malnutrition and low immunity, and increase the risk of complications such as infection. The generation of edema is closely related to the decrease in plasma colloidal osmotic pressure and the imbalance in the kidney's regulation of water and sodium metabolism. Severe edema will not only affect the patient's appearance and mobility, but may also lead to effusion of serous cavities such as the pleural and abdominal cavities, further increasing the burden on important organs such as the heart and lungs. The presence of hyperlipidemia reflects the abnormality of lipid metabolism in the patient's body, which will not only increase the risk of cardiovascular disease, but also cause further damage to the kidney itself.

[0003] At present, the treatment methods for nephrotic syndrome mainly include the use of glucocorticoids, immunosuppressants and other drugs. However, these treatment methods have many disadvantages. Although glucocorticoids can inhibit immune response and reduce kidney inflammation to a certain extent during treatment, long-term use can cause a series of serious adverse reactions, such as central obesity, osteoporosis, increased blood sugar, increased risk of infection, etc. These adverse reactions will not only affect the patient's quality of life, but may also lead to the emergence of other health problems. Immunosuppressants also have serious side effects, such as inhibiting the patient's bone marrow hematopoietic function, resulting in a decrease in the number of blood cells such as white blood cells and platelets, making the patient more susceptible to infection, and may also cause direct damage to important organs such as the liver and kidneys, further aggravating the patient's condition. In addition, long-term use of immunosuppressants may also increase the risk of tumors. Therefore, it is of great practical significance to develop a safe, effective and low-side effect method for treating nephrotic syndrome, which is also a key issue that needs to be urgently solved in the current field of kidney disease treatment. Summary of the invention

[0004] In order to achieve the above object, the following technical scheme is adopted: The present invention provides a Chinese medicine composition for treating nephrotic syndrome, which is prepared from the following components in parts by weight:

[0005] 10-15 parts of Anemarrhena Rhizoma, 10-15 parts of Phellodendron Amurense, 20-25 parts of Rehmannia glutinosa, 15-20 parts of Dioscorea Opposita, 10-15 parts of Paeonia Suffruticosa, 10-15 parts of Alismatis, 10g-15 parts of Poria, 60-100 parts of Astragalus, 10-15 parts of Angelica Sinensis, 15-20 parts of Cornus Officinalis, 10-20 parts of Panax Notoginseng, 10-15 parts of Magnolia Officinalis, 10-15 parts of Citrus Aurantium, 20-30 parts of Eucommia, 10-20 parts of Silkworm Cooter, 30-40 parts of Rosa Laevigata Fruit, 30-40 parts of Euryale ferox, 10-20 parts of Rubia cordifolia, 10-20 parts of Eclipta prostrata and 10-30 parts of Licorice.

[0006] Furthermore, the Chinese medicine composition is prepared by the following steps:

[0007] S1. Pretreatment of medicinal materials: Anemarrhena, Phellodendron, Rehmannia glutinosa, Dioscorea opposita, Paeonia suffruticosa, Alisma orientalis, Poria, Astragalus, Angelica sinensis, Cornus officinalis, Panax notoginseng, Magnolia officinalis, Citrus aurantium, Eucommia ulmoides, Silkworm Codonopsis pilosula, Rosa laevigata, Euryale ferox, Rubia cordifolia, Eclipta prostrata, and Licorice are weighed by weight, dried with hot air, and then crushed into coarse powder, passed through an 80-mesh sieve, and mixed evenly in a mixer to obtain a mixture;

[0008] S2. Extracting the active ingredient: The mixture was uniformly moistened with a first ethanol, placed at room temperature for 5 hours and then a second ethanol was added for impregnation, followed by percolation to obtain a percolation solution and recover the solvent;

[0009] S3. Concentration and mixing: The filtered liquid is concentrated under reduced pressure to obtain a block solid with a relative density of 1.22-1.32, which is ultrafinely ground and then passed through a 200-mesh sieve to obtain a fine powder, which is mixed evenly to obtain a Chinese medicine composition for treating nephrotic syndrome.

[0010] Furthermore, the drying conditions in step S1 are: drying at 65-75° C. for 2-4 hours and a wind speed of 0.5-2 m / s.

[0011] Furthermore, the mixing condition in step S1 is: mixing at a rotation speed of 25-30 rpm for 10-15 min.

[0012] Furthermore, in step S2, the volume fraction of the first ethanol is 60-75%, and the amount used is 3-4 times the mass of the mixture.

[0013] Furthermore, in step S2, the volume fraction of the first ethanol is 70-80%, and the amount used is 15-17 times the mass of the mixture.

[0014] Furthermore, the immersion conditions of step S2 are: immersion at 30-45° C. for 50-55 hours.

[0015] Furthermore, the percolation conditions of step S2 are 5-6 mL / min / kg medicinal material, and the percolation time is 45-55 h.

[0016] Furthermore, the ultrafine grinding conditions in step S3 are: temperature 20-40° C., and grinding time 25-35 min.

[0017] Furthermore, the mixing condition in step S3 is: mixing at a speed of 45-60 rpm for 15-40 min.

[0018] Furthermore, the Chinese medicine composition is an oral Chinese medicine preparation prepared from the Chinese medicine components.

[0019] Furthermore, the oral Chinese medicine preparation is a decoction, pill, tablet, mixture, capsule, granule, powder or paste.

[0020] The properties, flavors, meridians, effects and functions of each component in the Chinese medicine composition are as follows:

[0021] Anemarrhena: (1) It tastes bitter and sweet, is cold in nature, and enters the lung, stomach, and kidney meridians; (2) It clears away heat and purges fire, nourishes yin and moistens dryness; (3) In the treatment of nephrotic syndrome, Anemarrhena can clear the deficiency fire in the kidney meridian, relieve the discomfort caused by the deficiency fire burning the kidney yin, improve the state of kidney yin deficiency and excess fire, reduce inflammation-related fever symptoms, and create a good internal environment for kidney recovery.

[0022] Phellodendron amurense: (1) It tastes bitter and is cold in nature. It enters the kidney and bladder meridians. (2) It clears away heat and dampness, purges fire and eliminates steam, and detoxifies and treats sores. (3) For patients with nephrotic syndrome, Phellodendron amurense can effectively improve symptoms such as difficulty urinating caused by damp heat in the lower abdomen. It uses its anti-inflammatory and anti-allergic properties to reduce local inflammation in the kidneys and reduce the risk of kidney immune damage.

[0023] Rehmannia root: (1) Sweet in taste, slightly warm in nature, enters the liver and kidney meridians; (2) Nourishes yin and replenishes blood, replenishes essence and fills the marrow; (3) Nephrotic syndrome is often accompanied by kidney yin deficiency and insufficient essence and blood. Rehmannia root can nourish kidney yin, replenish the essence and blood needed by the kidneys, improve kidney function, regulate the body's immunity, and help the kidneys repair and maintain function.

[0024] Chinese Yam: (1) Sweet in taste, neutral in nature, enters the spleen, lung, and kidney meridians; (2) nourishes the spleen and stomach, promotes the production of body fluids and benefits the lungs, nourishes the kidneys and astringes the essence; (3) can regulate the disordered immune function of patients with nephrotic syndrome and enhance the body's resistance. At the same time, by nourishing the kidneys and astringing the essence, it can reduce proteinuria and alleviate the loss of protein in the kidneys.

[0025] Peony bark: (1) It tastes bitter and pungent, is slightly cold in nature, and enters the heart, liver, and kidney meridians; (2) It clears away heat and cools blood, and promotes blood circulation and removes blood stasis; (3) During treatment, peony bark removes heat and toxins from the blood, improves kidney blood circulation, prevents blood stasis, alleviates pathological damage to the kidneys caused by blood stasis and heat and toxins, and promotes normal kidney metabolism.

[0026] Alisma orientalis: (1) Sweet and light in taste, cold in nature, enters the kidney and bladder meridians; (2) It promotes diuresis and eliminates dampness, relieves heat and relieves stranguria; (3) Aiming at the edema symptoms of nephrotic syndrome, Alisma orientalis promotes the discharge of excess water in the body and relieves edema. Its heat-relieving and stranguria-relieving effects can clear dampness and heat in the lower jiao and improve the internal environment of the urinary system.

[0027] Poria: (1) It tastes sweet and light, is neutral in nature, and enters the heart, lung, spleen, and kidney meridians; (2) It promotes diuresis and eliminates dampness, strengthens the spleen, and calms the mind; (3) On the one hand, it promotes diuresis and reduces swelling, alleviating the symptoms of edema caused by kidney disease; on the other hand, it strengthens the spleen and calms the mind, regulates the patient's overall condition, and improves palpitations and insomnia caused by internal dampness, and its immunomodulatory effect helps restore immune balance.

[0028] Astragalus: (1) Sweet in taste, slightly warm in nature, enters the spleen and lung meridians; (2) replenishes qi and raises yang, consolidates the exterior and stops sweating, promotes diuresis and reduces swelling, promotes the production of body fluids and nourishes blood, relieves stagnation and arthritis, removes toxins and discharges pus, and heals sores and promotes tissue regeneration; (3) can enhance the resistance of patients with nephrotic syndrome and prevent infection. It promotes diuresis and reduces swelling, reduces edema, improves kidney blood circulation, promotes kidney function recovery, and enhances the body's self-repair ability.

[0029] Angelica sinensis: (1) It is sweet and pungent in taste, warm in nature, and enters the liver, heart, and spleen meridians; (2) It replenishes blood and activates blood circulation, regulates menstruation and relieves pain, and moistens the intestines and promotes bowel movements; (3) It improves kidney blood circulation and prevents the formation of blood stasis. The blood-replenishing function improves the symptoms of blood deficiency that may be caused by kidney disease, improves the overall state of the body, and provides nutritional support for kidney recovery.

[0030] Cornus officinalis: (1) It tastes sour and astringent, is slightly warm in nature, and enters the liver and kidney meridians; (2) It nourishes the liver and kidneys, and has astringent and firming properties; (3) It improves liver and kidney yin deficiency in patients with nephrotic syndrome, reduces the loss of proteinuria and other fine substances through astringent and firming properties, protects kidney function, and reduces nutrient loss.

[0031] Panax notoginseng: (1) Sweet, slightly bitter, warm in nature, enters the liver and stomach meridians; (2) Disperses blood stasis, stops bleeding, reduces swelling and relieves pain; (3) In the treatment of kidney disease, it improves kidney blood circulation, relieves blood stasis, and at the same time stops bleeding when hematuria is present, reducing kidney bleeding damage.

[0032] Magnolia officinalis: (1) It tastes bitter and pungent, is warm in nature, and enters the spleen, stomach, lung, and large intestine meridians; (2) It dries dampness and eliminates phlegm, and relieves gas and dissipates fullness; (3) Magnolia officinalis can effectively improve gastrointestinal function in patients with nephrotic syndrome who may experience gastrointestinal qi stagnation and dampness obstruction. Its anti-inflammatory effect also helps to reduce the body's overall inflammatory response.

[0033] Citrus aurantium: (1) It tastes bitter, pungent, and sour; it is warm in nature; it enters the spleen, stomach, and large intestine meridians; (2) it can dissipate qi and eliminate stagnation, resolve phlegm, and relieve abdominal distension; (3) Citrus aurantium excites gastrointestinal smooth muscle, promotes gastrointestinal motility, regulates gastrointestinal function in patients with nephrotic syndrome, facilitates the absorption of drugs and nutrients, and ensures normal metabolism of the body.

[0034] Eucommia ulmoides: (1) Sweet in taste, warm in nature, enters the liver and kidney meridians; (2) Nourishes the liver and kidneys, strengthens the tendons and bones, and stabilizes the fetus; (3) In the treatment of nephrotic syndrome, Eucommia ulmoides nourishes the liver and kidneys, enhances the body's resistance, improves symptoms such as soreness of the waist and knees caused by long-term illness, and improves the patient's quality of life.

[0035] Silkworm cocoons: (1) Sweet and salty in taste, neutral in nature, enter the liver and kidney meridians; (2) Strengthen semen and reduce urine, nourish the kidney and boost yang; (3) Help reduce proteinuria in patients with nephrotic syndrome, improve the kidney’s semen-retaining function, reduce the loss of protein and other fine substances in the kidney, and protect the kidney’s normal excretion function.

[0036] Rosa rugosa fruit pulp: (1) It tastes sour, sweet, and astringent, is neutral in nature, and enters the kidney, bladder, and large intestine meridians; (2) It consolidates semen and reduces urine, and it astringes the intestines to stop diarrhea; (3) It mainly reduces the loss of proteinuria and other fine substances, enhances the kidney's function of consolidating semen and reducing urine, promotes the recovery of kidney function in patients with nephrotic syndrome, and improves abnormal kidney excretion.

[0037] Gorgon fruit: (1) Sweet and astringent in taste, neutral in nature, enters the spleen and kidney meridians; (2) Strengthens the kidney and consolidates the essence, nourishes the spleen and stops diarrhea, removes dampness and stops leukorrhea; (3) Strengthens the kidney and consolidates the essence, reduces proteinuria, nourishes the spleen and stops diarrhea, regulates gastrointestinal function, comprehensively improves the physical condition of patients with nephrotic syndrome, and promotes overall functional recovery.

[0038] Rubia cordifolia: (1) It tastes bitter, is cold in nature, and enters the liver meridian; (2) It cools the blood, removes blood stasis, stops bleeding, and regulates menstruation; (3) It has a hemostatic effect on the hematuria symptoms that may occur in patients with nephrotic syndrome. Its blood stasis-removing function improves kidney blood circulation, reduces the adverse effects of blood stasis on the kidneys, and promotes kidney health.

[0039] Eclipta prostrata: (1) Sweet and sour in taste, cold in nature, enters the kidney and liver meridians; (2) nourishes the liver and kidneys, cools blood and stops bleeding; (3) improves liver and kidney yin deficiency in patients with nephrotic syndrome, cools blood and stops bleeding on possible hematuria, maintains normal kidney function, and relieves symptoms of kidney yin deficiency and bleeding.

[0040] Licorice: (1) Sweet in taste, neutral in nature, enters the heart, lung, spleen, and stomach meridians; (2) nourishes the spleen and replenishes qi, clears away heat and detoxifies, removes phlegm and relieves cough, relieves pain, and harmonizes various drugs; (3) regulates the body's immune function and reduces inflammatory reactions. As a harmonizing drug, it can make the various drugs in the prescription work better, reduce drug toxicity and adverse reactions, and ensure the safety and effectiveness of the prescription.

[0041] The beneficial effects of the present invention are as follows: the Chinese medicine composition disclosed in the present invention can effectively treat nephrotic syndrome, and through reasonable combination of multiple Chinese medicines, the synergistic effect of various Chinese medicines can intervene in the pathological mechanism of nephrotic syndrome from multiple links, and the astragalus can enhance the patient's resistance, prevent infection, promote diuresis and reduce swelling, improve kidney blood circulation, and promote kidney function recovery; the yam can regulate the patient's disordered immune function, reduce proteinuria, and alleviate kidney protein loss. A variety of drugs work together to comprehensively improve the pathological state of the kidneys and achieve the purpose of treating nephrotic syndrome. Traditional Chinese medicines such as mulberry silkworm cocoons, golden cherry pulp, and Euryale ferox have the effects of consolidating essence and reducing urine, and can effectively reduce the loss of fine substances such as protein in the kidneys, thereby reducing the level of proteinuria. The reduction of proteinuria is crucial to improving the condition of patients with nephrotic syndrome. It helps to reduce the burden on the kidneys and delay the progression of kidney disease. The present invention regulates the metabolic function of the kidneys, promotes the excretion of metabolic wastes such as creatinine, and reduces the content of creatinine in the blood, thereby reducing the metabolic pressure of the kidneys and protecting kidney function. In addition, compared with traditional treatment methods, the Chinese medicine composition of the present invention has no adverse reactions such as liver and kidney damage. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 The quantitative detection results of urine protein in each group of experimental animals on days 0, 7, 14, 21 and 28 of the present invention;

[0043] Figure 2 These are the test results of serum creatinine concentrations of experimental animals in each group of the present invention on days 0, 7, 14, 21 and 28.

[0044] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention. DETAILED DESCRIPTION

[0045] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0046] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0047] The experimental methods in the following examples are conventional methods unless otherwise specified, and the experimental materials used in the following examples are purchased from commercial channels unless otherwise specified.

[0048] Example 1

[0049] A Chinese medicine composition for treating nephrotic syndrome, comprising the following components in parts by weight:

[0050] 10 parts of Rhizoma Anemarrhenae, 10 parts of Cortex Phellodendri, 20 parts of Radix Rehmanniae Preparata, 15 parts of Rhizoma Dioscoreae, 10 parts of Cortex Moutan, 10 parts of Rhizoma Alismatis, 10 parts of Poria, 60 parts of Radix Astragali, 10 parts of Radix Angelicae Sinensis, 15 parts of Fructus Corni, 10 parts of Notoginseng, 10 parts of Cortex Magnoliae, 10 parts of Fructus Aurantii Immaturus, 20 parts of Eucommia Bark, 10 parts of Silkworm Cooter, 30 parts of Fructus Rosae Laevigatae, 30 parts of Fructus Euryale, 10 parts of Radix Rubiae, 10 parts of Ecliptae, and 10 parts of Radix Glycyrrhizae.

[0051] The Chinese medicine composition is prepared by the following steps:

[0052] S1. Pretreatment of medicinal materials: Weigh Rhizoma Anemarrhenae, Cortex Phellodendri, Radix Rehmanniae Preparata, Rhizoma Dioscoreae, Cortex Moutan, Rhizoma Alismatis, Poria, Radix Astragali, Radix Angelicae Sinensis, Fructus Corni, Radix Notoginseng, Cortex Magnoliae, Fructus Aurantii Immaturus, Eucommia ulmoides, Silkworm Codonopsis, Fructus Rosae Laevigatae, Fructus Euryale, Rubia cordifolia, Ecliptae, and Radix Glycyrrhizae by weight, dry at 65°C at a wind speed of 0.5 m / s for 2 h, then grind into coarse powder, pass through an 80-mesh sieve, place in a mixer, and mix at a speed of 25 rpm for 10 min to obtain a mixture;

[0053] S2. Extraction of active ingredients: uniformly moistening with a first ethanol having a volume fraction of 60% and an amount of 3 times the mass of the mixture, leaving at room temperature for 5 hours, adding a second ethanol having a volume fraction of 70% and an amount of 15 times the mass of the mixture, immersing at 30°C for 50 hours, and then percolating at a rate of 5 mL / min / kg of medicinal materials for 45 hours to obtain a percolated solution and recover the solvent;

[0054] S3. Concentration and mixing: The filtered liquid is concentrated under reduced pressure to obtain a block solid with a relative density of 1.22, which is ultrafinely ground at 20°C for 25 minutes, then sieved through a 200-mesh sieve to obtain a fine powder, and mixed at a speed of 45 rpm for 15 minutes to obtain a Chinese medicine composition for the treatment of nephrotic syndrome.

[0055] Example 2

[0056] A Chinese medicine composition for treating nephrotic syndrome, comprising the following components in parts by weight:

[0057] 15 parts of Rhizoma Anemarrhenae, 15 parts of Cortex Phellodendri, 25 parts of Radix Rehmanniae Preparata, 20 parts of Dioscorea Oppositae, 15 parts of Cortex Moutan, 15 parts of Rhizoma Alismatis, 15 parts of Poria, 100 parts of Radix Astragali, 15 parts of Radix Angelicae Sinensis, 20 parts of Fructus Corni, 20 parts of Notoginseng, 15 parts of Cortex Magnoliae Officinalis, 15 parts of Fructus Aurantii Immaturus, 30 parts of Eucommia Bark, 20 parts of Silkworm Cocoon, 40 parts of Fructus Rosae Laevigatae, 40 parts of Fructus Euryale, 20 parts of Radix Rubiae, 20 parts of Ecliptae, and 30 parts of Radix Glycyrrhizae.

[0058] The Chinese medicine composition is prepared by the following steps:

[0059] S1. Pretreatment of medicinal materials: Weigh Rhizoma Anemarrhenae, Cortex Phellodendri, Radix Rehmanniae Preparata, Rhizoma Dioscoreae, Cortex Moutan, Rhizoma Alismatis, Poria, Radix Astragali, Radix Angelicae Sinensis, Fructus Corni, Radix Notoginseng, Cortex Magnoliae, Fructus Aurantii Immaturus, Eucommia ulmoides, Silkworm Codonopsis, Fructus Rosae Laevigatae, Fructus Euryale, Rubia cordifolia, Ecliptae Ligusticum, and Radix Glycyrrhizae by weight, dry at 75°C at a wind speed of 2 m / s for 4 h, then grind into coarse powder, pass through an 80-mesh sieve, place in a mixer, and mix at a speed of 30 rpm for 15 min to obtain a mixture;

[0060] S2. Extraction of active ingredients: uniformly moistening with a first ethanol having a volume fraction of 75% and an amount of 4 times the mass of the mixture, placed at room temperature for 5h, adding a second ethanol having a volume fraction of 80% and an amount of 17 times the mass of the mixture, immersing at 45 ° C for 55h, and then percolating at a rate of 6mL / min / kg of medicinal materials for 55h to obtain a percolation solution and recover the solvent;

[0061] S3. Concentration and mixing: The filtered liquid is concentrated under reduced pressure to obtain a block solid with a relative density of 1.32, which is ultrafinely ground at 40°C for 35 minutes, then sieved through a 200-mesh sieve to obtain a fine powder, and mixed at a speed of 60 rpm for 40 minutes to obtain a Chinese medicine composition for the treatment of nephrotic syndrome.

[0062] Example 3

[0063] A Chinese medicine composition for treating nephrotic syndrome, comprising the following components in parts by weight:

[0064] 12.5 parts of Rhizoma Anemarrhenae, 12.5 parts of Cortex Phellodendri, 22.5 parts of Radix Rehmanniae Preparata, 17.5 parts of Rhizoma Dioscoreae, 12.5 parts of Cortex Moutan, 12.5 parts of Rhizoma Alismatis, 12.5 parts of Poria, 80 parts of Radix Astragali, 12.5 parts of Radix Angelicae Sinensis, 17.5 parts of Fructus Corni, 15 parts of Notoginseng, 12.5 parts of Cortex Magnoliae, 12.5 parts of Fructus Aurantii Immaturus, 25 parts of Eucommia Bark, 15 parts of Silkworm Cooter, 35 parts of Fructus Laevigatae, 35 parts of Fructus Euryale, 15 parts of Rubia Cordata, 15 parts of Eclipta Prostrata, and 20 parts of Radix Glycyrrhizae.

[0065] The Chinese medicine composition is prepared by the following steps:

[0066] S1. Pretreatment of medicinal materials: Weigh Rhizoma Anemarrhenae, Cortex Phellodendri, Radix Rehmanniae Preparata, Rhizoma Dioscoreae, Cortex Moutan, Rhizoma Alismatis, Poria, Radix Astragali, Radix Angelicae Sinensis, Fructus Corni, Radix Notoginseng, Cortex Magnoliae, Fructus Aurantii Immaturus, Eucommia ulmoides, Silkworm Codonopsis, Fructus Rosae Laevigatae, Fructus Euryale, Rubia cordifolia, Ecliptae, and Radix Glycyrrhizae by weight, dry at 70°C at a wind speed of 1.25 m / s for 3 h, then grind into coarse powder, pass through an 80-mesh sieve, place in a mixer, and mix at a speed of 27.5 rpm for 12.5 min to obtain a mixture;

[0067] S2. Extraction of active ingredients: uniformly moistening with a first ethanol having a volume fraction of 67.5% and an amount of 3.5 times the mass of the mixture, leaving at room temperature for 5 hours, adding a second ethanol having a volume fraction of 75% and an amount of 16 times the mass of the mixture, immersing at 37.5°C for 52.5 hours, and then percolating at a rate of 5.5 mL / min / kg of medicinal material for 50 hours to obtain a percolated solution and recover the solvent;

[0068] S3. Concentration and mixing: The filtered liquid was concentrated under reduced pressure to obtain a block solid with a relative density of 1.25, which was ultrafinely ground at 30°C for 30 minutes, then sieved through a 200-mesh sieve to obtain a fine powder, and mixed at a speed of 52.5 rpm for 27.5 minutes to obtain a Chinese medicine composition for the treatment of nephrotic syndrome.

[0069] Results Analysis

[0070] Analysis of the efficacy of Chinese herbal medicine combination in the treatment of nephrotic syndrome

[0071] Experimental animals: 6-week-old healthy male SD rats, weighing 120-130 g.

[0072] Modeling method: The rats were fixed on a special mouse board with tail vein injection of doxorubicin, and the tails were exposed. The tails of the rats were wiped with 75% alcohol cotton balls, the tail veins were dilated, and the needle was inserted into the distal 1 / 3 of the tail vein. The doxorubicin solution was slowly injected at a dose of 5 mg / kg body weight. After the injection of doxorubicin, the animals were returned to the breeding cages and continued to be raised and observed. One week after the injection of doxorubicin, the proteinuria of the animals was detected. The 24-hour urine of the animals was collected in a metabolic cage, and the urine protein content was detected by the biuret method. The model was successfully established when the 24-hour urine protein of the rats reached more than 100 mg.

[0073] Experimental groups:

[0074] Normal control group: 10 rats, without any modeling treatment, were given an equal volume of normal saline by gavage.

[0075] Model control group: 10 rats were given 2.5mL / kg normal saline by gavage twice a day for 4 weeks after successful modeling.

[0076] Low-dose Chinese medicine group: 10 rats were given a Chinese medicine composition at a dose of 2.5 mL / kg by gavage after successful modeling, twice a day for 4 consecutive weeks.

[0077] Chinese medicine medium dose group: 10 rats were given 5 mL / kg of Chinese medicine composition by gavage after successful modeling, twice a day for 4 consecutive weeks.

[0078] High-dose Chinese medicine group: 10 rats were given a Chinese medicine composition at a dose of 10 mL / kg by gavage after successful modeling, twice a day for 4 consecutive weeks.

[0079] Positive control group: 10 rats were given 2.5mL / kg of positive control drug such as Tripterygium wilfordii polyglycosides tablets by gavage after successful modeling, twice a day for 4 consecutive weeks.

[0080] Analysis method: 24-hour urine was collected on days 0, 7, 14, 21, and 28 of administration, and the 24-hour urine protein was measured by the biuret method. The results are shown in Figure 1 .

[0081] On the 0th, 7th, 14th, 21st and 28th days of administration, blood was collected from the experimental animals through the retro-orbital venous plexus, and serum was obtained after centrifugation. The serum creatinine concentration was detected by the picric acid method. The results are shown in Figure 2 .

[0082] from Figure 1 It can be seen that the urine protein quantification of the rats in the normal control group was maintained at a low and stable level throughout the experiment, which indicates that the renal function of normal rats is normal and there is no proteinuria. After the modeling is successful, the urine protein quantification of the rats in the model control group increased significantly and remained at a high level throughout the 4-week observation period, which reflects the effectiveness of the nephrotic syndrome model, that is, the renal filtration function is impaired after the modeling is successful, resulting in a large amount of protein being lost from the urine. After the Chinese medicine composition was gavaged, the urine protein quantification of the low-dose group, the medium-dose group and the high-dose group showed a gradual downward trend over time, among which the high-dose group had the most obvious decline, and its urine protein quantification level was significantly lower than that of the model control group, and compared with the positive control group, the high-dose group had a more prominent effect in reducing urine protein quantification, indicating that the Chinese medicine composition of the present invention has a significant advantage in improving the proteinuria symptoms caused by nephrotic syndrome when used at a high dose. The low-dose group and the medium-dose group also showed a certain degree of effect in reducing urine protein, indicating that the Chinese medicine composition of the present invention has a regulating effect on urine protein at different doses, and there is a certain dose dependence.

[0083] Figure 2It can be seen that the serum creatinine concentration of rats in the normal control group was maintained within the normal range and was relatively stable, reflecting that the normal rats had good renal metabolic function and could effectively remove metabolic wastes such as creatinine from the body. The serum creatinine concentration of rats in the model control group increased significantly after modeling, indicating that the renal metabolic function of rats with nephrotic syndrome model was impaired, and creatinine excretion was blocked, resulting in an increase in serum creatinine concentration. After medication, the serum creatinine concentration of the low-dose group, the medium-dose group and the high-dose group of Chinese medicine gradually decreased. The effect of the high-dose group of Chinese medicine on reducing serum creatinine concentration was the most significant, and was significantly lower than that of the positive control group. This shows that the Chinese medicine composition of the present invention can more effectively promote the excretion of metabolic wastes such as creatinine at a high dose, reduce the creatinine content in the blood, reduce renal metabolic pressure, and protect renal function. The low-dose group and the medium-dose group of Chinese medicine also have a positive effect on reducing serum creatinine concentration, further confirming that the Chinese medicine composition of the present invention can regulate renal metabolic function at different doses and improve the renal pathological state of rats with nephrotic syndrome.

[0084] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0085] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A Chinese medicine composition for treating nephrotic syndrome, characterized in that: Made from the following components by weight: 10-15 parts of Rhizoma Anemarrhenae, 10-15 parts of Cortex Phellodendri, 20-25 parts of Radix Rehmanniae Preparata, 15-20 parts of Rhizoma Dioscoreae, 10-15 parts of Cortex Moutan, 10-15 parts of Rhizoma Alismatis, 10g-15 parts of Poria, 60-100 parts of Radix Astragali, 10-15 parts of Radix Angelicae Sinensis, 15-20 parts of Fructus Corni, 10-20 parts of Radix Notoginseng, 10-15 parts of Cortex Magnoliae Officinalis, 10-15 parts of Fructus Aurantii Immaturus, 20-30 parts of Eucommia Bark, 10-20 parts of Ocellus Officinalis, 30-40 parts of Fructus Laevigatae, 30-40 parts of Fructus Euryale, 10-20 parts of Rubia Cordata, 10-20 parts of Eclipta Prostrata and 10-30 parts of Radix Glycyrrhizae; The Chinese medicine composition is prepared by the following steps: S1. Pretreatment of medicinal materials: Anemarrhena, Phellodendron, Rehmannia glutinosa, Dioscorea opposita, Paeonia suffruticosa, Alisma orientalis, Poria, Astragalus, Angelica sinensis, Cornus officinalis, Panax notoginseng, Magnolia officinalis, Citrus aurantium, Eucommia ulmoides, Silkworm Codonopsis pilosula, Rosa laevigata, Euryale ferox, Rubia cordifolia, Eclipta prostrata, and Licorice are weighed by weight, dried with hot air, and then crushed into coarse powder, passed through an 80-mesh sieve, and mixed evenly in a mixer to obtain a mixture; S2. Extracting the active ingredient: The mixture was uniformly moistened with a first ethanol, placed at room temperature for 5 hours and then a second ethanol was added for impregnation, followed by percolation to obtain a percolation solution and recover the solvent; S3. Concentration and mixing: The filtered liquid is concentrated under reduced pressure to obtain a block solid with a relative density of 1.22-1.32, which is ultrafinely ground and then passed through a 200-mesh sieve to obtain a fine powder, which is mixed evenly to obtain a Chinese medicine composition for treating nephrotic syndrome.

2. The Chinese medicine composition for treating nephrotic syndrome according to claim 1, characterized in that: The drying conditions in step S1 are: drying at 65-75° C. for 2-4 hours and a wind speed of 0.5-2 m / s.

3. The Chinese medicine composition for treating nephrotic syndrome according to claim 2, characterized in that: The mixing condition in step S1 is: mixing at a rotation speed of 25-30 rpm for 10-15 min.

4. The Chinese medicine composition for treating nephrotic syndrome according to claim 3, characterized in that: The volume fraction of the first ethanol in step S2 is 60-75%, and the amount used is 3-4 times the mass of the mixture.

5. The Chinese medicine composition for treating nephrotic syndrome according to claim 4, characterized in that: In step S2, the volume fraction of the first ethanol is 70-80%, and the amount used is 15-17 times the mass of the mixture.

6. The Chinese medicine composition for treating nephrotic syndrome according to claim 5, characterized in that: The immersion conditions of step S2 are: immersion at 30-45° C. for 50-55 hours.

7. The Chinese medicine composition for treating nephrotic syndrome according to claim 6, characterized in that: The percolation conditions of step S2 are 5-6 mL / min / kg medicinal material, and the percolation time is 45-55 h.

8. The Chinese medicine composition for treating nephrotic syndrome according to claim 7, characterized in that: The ultrafine grinding conditions in step S3 are: temperature 20-40° C., and grinding time 25-35 min.

9. The Chinese medicine composition for treating nephrotic syndrome according to claim 8, characterized in that: The mixing condition in step S3 is: mixing at a speed of 45-60 rpm for 15-40 min.

10. The Chinese medicine composition for treating nephrotic syndrome according to claim 9, characterized in that: The Chinese medicine composition is a Chinese medicine oral preparation prepared from the Chinese medicine components, and the Chinese medicine oral preparation is a decoction, pill, tablet, mixture, capsule, granule, powder or paste.

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