Application of 1-methoxy-2-propanol as active ingredient in preparation of mosquito luring agent

Abstract

The invention belongs to the technical field of mosquito trapping, and particularly relates to application of 1-methoxy-2-propanol as an active ingredient in preparation of a mosquito trapping agent. The method is suitable for popularization and application. The mosquito luring agent 1-methoxy-2-propanol is derived from volatile matters released by human beings and mice under natural conditions, can simulate the release mode of host volatile matters, makes up for the defects of existing biological control, and has a remarkable attraction effect.

Description

Technical Field

[0001] The invention belongs to the technical field of mosquito attractants, and particularly relates to application of 1-methoxy-2-propanol as an active ingredient in the preparation of mosquito attractants. Background Art

[0002] Mosquito-borne diseases, including malaria, dengue, chikungunya, Zika, West Nile fever, Japanese encephalitis, and lymphatic filariasis, continue to wreak havoc around the world, killing millions of people each year. In recent years, the threat of mosquito-borne diseases has intensified with global warming, globalization, and accelerated urbanization. Although mosquito control remains the main means of preventing and controlling these diseases, traditional physical methods are environmentally friendly but have limited effects, and chemical methods face difficulties due to the increasing resistance of mosquitoes. Therefore, there is an urgent need to develop new mosquito control strategies to meet this challenge.

[0003] Aedes aegypti was originally distributed in Africa, but has now expanded to the entire tropical and part of the temperate zone. It is distributed in Hainan, Yunnan, Guangxi, Guangdong and other provinces in China. It is the main vector of urban yellow fever, chikungunya and dengue fever, and is recognized as one of the most dangerous mosquito species. In the past 20 years, the number of cases of these diseases has increased rapidly around the world, becoming an important global public health issue. With the continuous increase in dengue cases in Southeast Asian countries and the increase in the importation of dengue cases from neighboring countries, many local outbreaks of diseases transmitted by Aedes aegypti have occurred, posing challenges to the national public health system.

[0004] Culex pipiens pallens is an important vector of West Nile fever, Japanese encephalitis and filariasis, and is widely distributed in areas north of 32° north latitude in China.

[0005] The odors emitted by different hosts have their own particularities. In order to selectively attract mosquitoes that prefer different hosts, adding some specific compounds can trap mosquito vectors more accurately and efficiently.

[0006] 1-Methoxy-2-propanol is an important organic compound with good solubility. It is miscible with water and a variety of organic solvents, so it is often used as a solvent and dispersant. It is also widely used in the packaging of electronic components and the preparation of insulating materials because it improves the solubility, fluidity and coating properties of epoxy resins. However, its application in the field of mosquito trapping technology has received little attention and needs to be developed urgently. Summary of the invention

[0007] In view of the shortcomings of the existing problems, the purpose of the present invention is to provide the use of 1-methoxy-2-propanol as an active ingredient in the preparation of mosquito attractants.

[0008] The technical solution adopted by the present invention to solve the technical problem is:

[0009] In a first aspect, the present invention protects the use of 1-methoxy-2-propanol as an active ingredient in the preparation of mosquito attractants.

[0010] In a second aspect, the present invention protects the use of 1-methoxy-2-propanol as a synergist in the preparation of mosquito attractants.

[0011] In a specific embodiment, the 1-methoxy-2-propanol has a structure shown in the following formula (I):

[0012]

[0013] In a specific embodiment, the inducer may also contain other active agents or related active ingredients for catching mosquitoes.

[0014] In a specific embodiment, the inducer further contains other carriers or excipients.

[0015] In specific embodiments, the mosquito is Aedes aegypti and / or Culex pipiens pallens.

[0016] In a specific embodiment, the solvent in the mosquito attractant is a volatile solvent.

[0017] In a specific embodiment, the solvent is n-hexane.

[0018] In a specific embodiment, the concentration of 1-methoxy-2-propanol is 0.05-5 μg / μL, such as 0.05-1 μg / μL, 0.8-2.5 μg / μL, 1.0-5 μg / μL, etc.

[0019] In a more specific embodiment, the concentration of 1-methoxy-2-propanol is 0.1-1 μg / μL, such as 0.1 μg / μL, 0.5 μg / μL, 0.8 μg / μL, 1 μg / μL, etc.

[0020] In a more specific embodiment, the concentration of 1-methoxy-2-propanol in the mosquito attractant for attracting Aedes aegypti is 0.1 μg / μL.

[0021] In a more specific embodiment, the concentration of 1-methoxy-2-propanol when the mosquito attractant attracts Culex pipiens pallens is 1 μg / μL.

[0022] Beneficial Effects

[0023] The 1-methoxy-2-propanol provided by the present invention, which can be used as the main active ingredient of the mosquito attractant, is obtained based on the screening of volatiles of the body flora of mice and has the following advantages:

[0024] The mosquito attractant 1-methoxy-2-propanol in the present invention is derived from volatiles released by humans and mice under natural conditions, can simulate the release pattern of host volatiles, make up for the shortcomings of existing biological control, and play a significant attracting effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 For the invention based on Pou2f3 - / - Screening results of volatiles from the mouse surface flora.

[0026] Figure 2 The present invention shows the behavioral screening results of the mosquito attractant 1-methoxy-2-propanol using a three-cage olfactometer device.

[0027] Figure 3 The present invention provides the dosage screening and mosquito attracting results of the mosquito attractant 1-methoxy-2-propanol after it is placed in a mosquito-killing lamp. DETAILED DESCRIPTION

[0028] The present invention is further described in detail below with reference to the examples. The reagents or instruments used without indicating the manufacturer are all regarded as conventional products that can be purchased on the market.

[0029] The present invention provides the use of 1-methoxy-2-propanol as a mosquito attractant component, wherein the active ingredient of the mosquito attractant contains 1-methoxy-2-propanol. The active ingredient refers to a component that exerts an attractant effect, relative to excipients, solvents, and other attractant components that do not interact with each other and other substances that have no attractant effect. All components of the mosquito attractant can be used as conventional commercial products. The use includes: using 1-methoxy-2-propanol to prepare a mosquito attractant product, which can be used as an attractant or an attractant device; using 1-methoxy-2-propanol as a mosquito attractant, which can be used directly to utilize a single volatile substance of 1-methoxy-2-propanol, or it can be used in the form of a composition, or it can be used through a volatilization device. In short, as long as various direct or indirect uses that utilize the function of 1-methoxy-2-propanol to attract mosquitoes belong to the technical solution to be protected by the present invention.

[0030] Example 1 Screening of volatile metabolites of the surface flora of highly attractive mice (Pou2f3- / - mice, KO mice):

[0031] For mice whose skin odor was experimentally verified to be more attractive to mosquitoes, the odor emitted by their skin was collected and flavoromics was detected by gas chromatography-mass spectrometry. The relative odor activity values ​​(ROAV) of the odor of 1-methoxy-2-propanol in highly attractive mice were the largest, and its ROVA value increased most significantly in highly attractive mice, indicating that 1-methoxy-2-propanol contributed more to the odor emitted by highly attractive mice. The relative content of 1-methoxy-2-propanol in highly attractive mice was also significantly higher than that in wild-type mice. The experimental results are shown in Figure 1 .

[0032] Example 2 Test of the attractant effect of single component 1-methoxy-2-propanol in a three-cage olfactometer apparatus:

[0033] 1. Preparation of mosquito attractants

[0034] Purchase 1-methoxy-2-propanol stock solution and n-hexane stock solution. Prepare a 1 μg / μL compound solution using n-hexane as solvent and 1-methoxy-2-propanol as solute.

[0035] 1-Methoxy-2-propanol: Manufacturer: Sigma; Product No.: 484407; Specification: 1L

[0036] n-Hexane production: Manufacturer: Sigma; Item No.: 270504; Specification: 4L

[0037] 2. Three-cage olfactory attraction experiment

[0038] Three anesthetized wild-type mice were placed on each side of the device, and 10 μL of mosquito attractant solution and 10 μL of n-hexane solution were added respectively. 60 female Culex pipiens were placed in the middle cage. After 30 minutes of adaptation, the gates on both sides were opened, and the number of female mosquitoes on both sides was counted for 30 minutes. This was repeated three times. The experimental results are shown in Figure 2 The average attraction rate of mice to mosquitoes added with the mosquito attractant solution was 84.72%, while the average attraction rate of mice to mosquitoes added with the n-hexane solution was 15.28%, indicating that the mosquito attractant solution has a significant attraction effect on mosquitoes.

[0039] Example 3 Screening of effective working concentration of 1-methoxy-2-propanol when used in mosquito killer lamps:

[0040] 1. Preparation of mosquito attractants

[0041] Purchase 1-methoxy-2-propanol stock solution and n-hexane stock solution. Use n-hexane as solvent and 1-methoxy-2-propanol as solute to prepare solutions of different concentrations, which are 0.01 μg / μL, 0.1 μg / μL, and 1 μg / μL, respectively.

[0042] 2. The source of the volatile liquid imitating human skin: Green Yinglu mosquito trap

[0043] 3. Use 1-methoxy-2-propanol and human skin volatile liquid to carry out the luring experiment

[0044] Description of the experimental process: Use mosquito nets to build a 3m*1.5m*2m space indoors. Put the cotton balls soaked with the mosquito attractant solutions and the mosquito traps into the mosquito traps. Put the three mosquito traps and the mosquito trap with only the mosquito traps in the four corners of the space, and release Aedes aegypti or Culex pipiens in the center. Turn on the mosquito traps and count the number of mosquitoes in each mosquito trap after 2 hours. Swap the mosquito traps after each experiment to eliminate the influence of spatial effects.

[0045] The concentration of 1-methoxy-2-propanol acting on Aedes aegypti is 0.1μg / μL. The relationship between the concentration of 1-methoxy-2-propanol and the attractant effect is relatively obvious, and it has a good attractant effect at a concentration of 0.1μg / μL. The concentration of 1-methoxy-2-propanol acting on Culex pipiens pallens is 1μg / μL. The relationship between the concentration of 1-methoxy-2-propanol and the attractant effect is relatively obvious, and it has a good attractant effect at a concentration of 1μg / μL. For specific experimental results, see Figure 3 .

[0046] The protection content of the present invention is not limited to the above embodiments. Without departing from the spirit and scope of the inventive concept, changes and advantages that can be thought of by those skilled in the art are included in the present invention and are protected by the attached claims.

Claims

1. Application of 1-methoxy-2-propanol as an active ingredient in the preparation of mosquito attractants.

2. Application of 1-methoxy-2-propanol as a synergist in the preparation of mosquito attractants.

3. The use according to claim 1 or 2, characterized in that: The 1-methoxy-2-propanol has a structure shown in the following formula (I):

4. The use according to claim 1, characterized in that: The mosquito is Aedes aegypti and / or Culex pipiens pallens.

5. The use according to claim 4, characterized in that: The solvent in the mosquito attractant is a volatile solvent.

6. The use according to claim 5, characterized in that: The solvent is n-hexane.

7. The use according to claim 1, characterized in that: The concentration of the 1-methoxy-2-propanol is 0.05 to 5 μg / μL.

8. The use according to claim 1, characterized in that: The concentration of the 1-methoxy-2-propanol is 0.1 to 1 μg / μL.

9. The use according to claim 8, characterized in that: The concentration of 1-methoxy-2-propanol in the mosquito attractant for attracting Aedes aegypti is 0.1 μg / μL.

10. The use according to claim 8, characterized in that: The 1-methoxy-2-propanol concentration of the mosquito attractant when attracting Culex pipiens pallens is 1 μg / μL.

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