Pesticide composition containing Xinjunan acetate

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition containing Xinjunan acetate. The invention relates to a pesticide composition containing Xinjunan acetate. The effective component of the pesticide composition is formed by compounding Xinjunan acetate and phenazino-1-carboxylic acid or berberine, the compounding mass ratio of the xinjunan acetate to the phenazino-1-carboxylic acid or the berberine is (1-150): (150-1). The pesticide composition disclosed by the invention is formed by compounding the xinjunan acetate and the phenazino-1-carboxylic acid or berberine, and the compounded composition shows a synergistic effect in a certain mass ratio, so that the prevention and treatment effect on diseases can be improved; besides, the action mechanisms of the xinjunan acetate and the phenazino-1-carboxylic acid or berberine are different, and the compound combination can perform multi-site action on pathogenic bacteria, so that the development of drug resistance of the pathogenic bacteria is delayed, and the resistance risk is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and in particular relates to a pesticide composition containing octamidine acetate. Background Art

[0002] Sinclofenac acetate (a form of sinclofenac) is a low-toxic, broad-spectrum fungicide developed in my country, mainly used in agriculture to prevent and treat diseases of various crops. Sinclofenac acetate can interfere with the fatty acid synthesis of bacterial and fungal cell membranes and destroy the structure of cell membranes; in addition, it denatures the enzyme system of pathogens by coagulating pathogen proteins and inhibiting the respiration of pathogens; secondly, in aqueous solution, sinclofenac acetate can produce ionization, and its hydrophilic group contains strong positive charge, which can adsorb pathogens that are usually negatively charged and inhibit their reproduction. Sinclofenac acetate has a good killing effect on various fungi, bacteria and viruses that cause crop diseases.

[0003] Shenqinmycin is a green pesticide of microbial origin developed in my country. Its main component is phenazine-1-carboxylic acid, a secondary metabolite produced by the plant rhizosphere growth-promoting Pseudomonas M18. Shenqinmycin belongs to the aromatic heterocyclic phenazine compound family. Its antibacterial activity has at least two mechanisms: first, during the reduction process of these compounds in pathogenic bacteria cells, toxic superoxide ions and hydrogen peroxide will be produced, which can oxidize glutathione and transferrin to produce highly cytotoxic hydroxyl free radicals; second, because phenazine compounds can be reduced by NADH and become intermediates of electron transfer, they disrupt the normal redox homeostasis in cells, affect energy production, and thus inhibit the growth of pathogenic microorganisms. Shenqinmycin can be used to prevent and control fungal, bacterial and nematode diseases of a variety of crops.

[0004] Berberine, also known as berberine, is an isoquinoline alkaloid, mainly extracted from the roots and stems of plants in the Coptis family. In clinical medicine, berberine has inhibitory effects on a variety of bacteria, fungi and viruses, such as Candida albicans, Escherichia coli, Staphylococcus aureus and influenza virus; and berberine can also improve the blood sugar and blood lipid levels of diabetic patients, and has hypoglycemic and lipid-regulating effects; secondly, berberine also has cardiovascular protection and anti-tumor effects. In terms of crop disease and insect pest control, existing technologies have reported that berberine can be used to prevent and control bacterial diseases of crops.

[0005] In the process of crop production, long-term continuous use of a single pesticide can easily lead to the development and rapid accumulation of pathogen resistance, resulting in reduced control effectiveness. The development of new active compounds is long, difficult, and costly. However, by rationally compounding existing active compounds and screening for synergistic combinations, while improving the control effect, it can also delay the development of pathogen resistance, extend the service life of the agent, and improve the utilization rate of existing active compounds. Summary of the invention

[0006] The purpose of the present invention is to provide a pesticide composition containing cintamidine acetate to solve the problems that in the process of crop production, long-term continuous use of a single pesticide easily causes the generation and rapid accumulation of pathogen resistance, resulting in reduced control efficiency; and the development of new active compounds has a long cycle, great difficulty and high cost.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A pesticide composition containing cincomycin acetate, wherein the active ingredient is prepared by compounding cincomycin acetate with sinocampus quinoline or berberine; the compounding mass ratio of cincomycin acetate to sinocampus quinoline or berberine is 1-150:150-1.

[0009] Furthermore, the composite mass ratio of the sinopyridamole acetate to sinopyridamycin is 1-19:29-1.

[0010] Furthermore, the composite mass ratio of the sphenanthracene acetate to berberine is 1-9:9-1.

[0011] The present invention also provides the use of the pesticide composition of octamidine acetate in preventing and controlling fungal diseases or bacterial diseases of crops.

[0012] The present invention also provides a pesticide preparation, which comprises the pesticide composition containing octamidine acetate.

[0013] Furthermore, in the pesticide preparation, the mass of the pesticide composition containing octamidine acetate accounts for 2-38% of the total mass of the pesticide preparation, and the rest are auxiliary ingredients allowed to be added in pesticide science.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] The pesticide composition of the present invention is prepared by compounding cintamidine acetate with sinocycline or berberine, and the compound combination shows a synergistic effect within a certain mass ratio, which can improve the control effect of diseases; in addition, cintamidine acetate and sinocycline or berberine have different action mechanisms, and the compound combination can act on pathogens at multiple sites, which helps to delay the development of pathogen resistance and reduce resistance risks. DETAILED DESCRIPTION

[0016] The technical solution of the present invention is described clearly and completely below. Based on the embodiments of the present invention, all other embodiments obtained by the technicians in this field without creative work are within the scope of protection of the present invention.

[0017] 1. Indoor biological activity test

[0018] (Refer to "NT / T 1156.16-2008 Pesticide Indoor Bioassay Test Guidelines Fungicide Part 16: Turbidimetric Method for Inhibition of Bacterial Growth")

[0019] 1.1 Test pathogens

[0020] Xanthomonas oryzae pv.oryzicola, isolated from diseased rice plants.

[0021] 1.2 Experimental culture medium

[0022] LB medium: 10 g tryptone, 5 g yeast extract, 10 g sodium chloride, distilled water to 1000 mL, 10 mol / L NaOH to adjust the pH to 7.4.

[0023] 1.3 Test Agents

[0024] 5% cintamidine acetate aqueous solution (Shaanxi Yinong High-Tech Pharmaceutical Co., Ltd.), 1% sinocycline suspension (Shanghai Nong Le Biological Products Co., Ltd.), 0.5% berberine aqueous solution (Hebei Wante Biochemical Co., Ltd.)

[0025] After the test agent is diluted with 0.1% Tween-80 aqueous solution to a high mass concentration single-dose stock solution, multiple groups of proportions are set, and each single-dose stock solution and the proportioned mixture are diluted with 0.1% Tween-80 aqueous solution to 5 mass concentration gradients. The agent in this test is prepared and used immediately.

[0026] 1.4 Test methods

[0027] Mix 1 mL of drug solution and 25 mL of LB medium to make drug-containing LB medium. Set 5 replicates for each concentration of drug solution, and set the treatment with an equal amount of 0.1% Tween-80 aqueous solution as a blank control. Pick a single colony of the test pathogen with an inoculation loop and place it in the LB medium. Shake and culture at 28°C and 130 r / min until the OD 600 100 μL of bacterial solution was inoculated into the drug-containing LB medium and the blank control LB medium respectively, and then placed at 28°C and 130 r / min for shaking culture until the blank control OD 600 The value reached about 0.5, and the OD of each NB culture medium after cultivation was measured by a spectrophotometer. 600 value.

[0028] In order to eliminate the error caused by the drug itself, the OD of sterile NB culture medium with different concentrations of drug solution was measured. 600 The OD value of the sterile NB medium of the blank control was 600 The OD value caused by the drug itself was corrected. The growth inhibition rate of the tested pathogens treated with different drugs was calculated.

[0029] Corrected OD 600 Value = OD of LB medium containing bacteria 600 Value- Sterile LB medium OD 600 value

[0030]

[0031] 4. Data Analysis

[0032] The EC of each treatment agent was calculated by linear regression analysis with the logarithmic value of the agent concentration as x and the corresponding probability value of growth inhibition as y. 50 The Sun Yunpei method was used to calculate the co-toxicity coefficient of the mixture, and the synergistic effect of the mixed drugs was evaluated based on the co-toxicity coefficient (CTC), that is, CTC≤80 was antagonistic, 80<CTC<120 was additive, and CTC≥120 was synergistic. The results are shown in Table 1-3.

[0033] Table 1 Results of toxicity test of single agent against rice bacterial leaf streak pathogen

[0034]

[0035] Table 2 The results of toxicity test of the combination of cintamidine acetate and sinocycline against rice bacterial leaf streak pathogen

[0036] Drug name and ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Sintramycin acetate 1: Sintramycin 29 9.1526 703.4919 546.4295 128.7434 Sintramycin acetate 1: Sintramycin 19 7.8752 817.6021 538.7325 151.7640 Sintramycin acetate 1: Sintramycin 9 10.2859 625.9812 515.6413 121.3986 Sintramycin acetate 1: Sintramycin 1 15.4514 416.7118 330.9118 125.9283 Sintramycin acetate 9: Sintramycin 1 23.0493 279.3482 146.1824 191.0957 Sintramycin acetate 19: Sintramycin 1 34.8736 184.6319 123.0912 149.9961 Sintramycin acetate 29: Sintramycin 1 52.6419 122.3128 115.3941 105.9957

[0037] As shown in Table 2, when cintamidine acetate is combined with sinopyrimidine, the co-toxicity coefficient against rice bacterial leaf streak pathogen is between 105.9957 and 191.0957. When the combined mass ratio of cintamidine acetate to sinopyrimidine is 1:29, 1:19, 1:9, 1:1, 9:1 and 19:1, a synergistic effect is exhibited.

[0038] Table 3 The results of toxicity test of the combination of cintoconazole acetate and berberine against rice bacterial leaf streak pathogen

[0039] Drug name and ratio <![CDATA[EC 50 (mg / L)]]> ATI TTI CTC Sintracin acetate 1: berberine 29 3.1916 2017.4145 2588.4370 77.9395 Sintracin acetate 1: berberine 19 2.7581 2334.4984 2545.5329 91.7096 Sintracin acetate 1: berberine 9 2.0693 3111.5740 2416.8206 128.7466 Sintracin Acetate 1: Berberine 1 3.7305 1725.9831 1387.1226 124.4290 Sintracin acetate 9: berberine 1 11.0720 581.5372 357.4245 162.7021 Sintracin acetate 19: berberine 1 24.0374 267.8651 228.7123 117.1188 Sintracin acetate 29: berberine 1 38.8440 165.7600 185.8082 89.2103

[0040] As shown in Table 3, when cincomycin acetate is combined with berberine, the co-toxicity coefficient against rice bacterial leaf streak pathogen is between 77.9395 and 162.7021. When the combined mass ratio of cincomycin acetate to berberine is 1:9, 1:1, and 9:1, a synergistic effect is exhibited.

[0041] In summary, when the present invention compounds cintamidine acetate with sinocampus or berberine, the compound combination exhibits a synergistic effect within a certain mass ratio, which can improve the control effect of diseases; in addition, cintamidine acetate and sinocampus or berberine have different action mechanisms, and the compound combination can act on pathogens at multiple sites, which helps to delay the development of drug resistance of pathogens and reduce the risk of resistance.

Claims

1. A pesticide composition containing octamidine acetate, characterized in that: The effective component is prepared by compounding cintamidine acetate and sinocampus quinacridone or berberine; the compounding mass ratio of cintamidine acetate to sinocampus quinacridone or berberine is 1-150:150-1.

2. The pesticide composition containing octamidine acetate according to claim 1, characterized in that: The composite mass ratio of the sinopyridamole acetate to sinopyridamycin is 1-19:29-1.

3. The pesticide composition containing octamidine acetate according to claim 1, characterized in that: The composite mass ratio of the sphenanthracene acetate to berberine is 1-9:9-1.

4. Use of the pesticide composition of cefotaxime acetate according to any one of claims 1 to 3 in preventing and controlling fungal or bacterial diseases of crops.

5. A pesticide preparation, characterized in that: The pesticide preparation comprises the pesticide composition containing octamidine acetate as claimed in claim 1.

6. The pesticide formulation according to claim 5, characterized in that: In the pesticide preparation, the mass of the pesticide composition containing octamidine acetate accounts for 2-38% of the total mass of the pesticide preparation, and the rest are auxiliary components allowed to be added in pesticide science.

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