Phagostimulant composition for codling moth larvae and application of phagostimulant composition

Abstract

The invention discloses a phagostimulant composition for codling moth larvae and application of the phagostimulant composition. The isoquercitrin and phlorizin compound preparation is prepared from the following components in parts by weight: 0.02 to 0.07 part of isoquercitrin, 0.6 to 0.9 part of phlorizin, 0.01 to 0.02 part of cyanidin-3-O-galactoside and 0.05 to 0.12 part of delphinidin-3-O-galactose. The phagostimulant provided by the invention is small in component dosage, low in price, capable of improving the behavior regulation effect of a lure on larvae and reducing the manufacturing cost, and suitable for popularization and promotion.

Description

Technical Field

[0001] The invention belongs to the technical field of pest trapping and killing, and particularly relates to an attractant composition for codling moth larvae and application thereof. Background Art

[0002] The codling moth (Cydia pomonella) is one of the most important fruit tree pests in the world. In China, it is mainly distributed in northern fruit tree planting areas, such as Xinjiang, Gansu, Ningxia, Hebei, Shanxi and Shaanxi. The occurrence in Xinjiang is particularly serious, and it is also scattered in some southern regions (such as Sichuan and Yunnan). Its larvae bore into the fruit, causing holes, rotting or falling off, which seriously affects the commodity value and the income of fruit farmers. At the same time, it increases the risk of disease transmission, causing fruit tree production reduction or even crop failure. The codling moth has the characteristics of overlapping generations and strong flight ability. It has 2-4 generations a year, spreads rapidly and is difficult to control. It also has strong drug resistance, which gradually reduces the effectiveness of traditional chemical control.

[0003] The fruit-boring nature of codling moth larvae poses a significant challenge to the control of chemical pesticides and biological agents. After hatching, the larvae quickly bore into the fruit, reducing the chance of direct contact between the pesticide and the fruit. At the same time, the fruit skin and internal tissues provide a physical barrier for the larvae, hindering the penetration and ingestion of pesticides, especially contact-killing chemical pesticides and biological agents that require ingestion, which significantly limit their effectiveness. In addition, the boring behavior enhances the concealment of the pest, increasing the difficulty of monitoring and control, often leading to delays in control and the spread of pests.

[0004] Whether there is an ideal method to regulate feeding behavior has a certain impact on insecticide. Attractants can slow down the speed of pests boring, thereby increasing the duration of action of the agent, and gather them in a position where the chemical agent directly acts. Attractants can be used to regulate the behavior of codling moth larvae to increase the control effect. Compared with the lure of sex attractants, attractants are more selective, lasting and stable for more harmful larvae, and are easy to operate and promote. In addition, attractants can be used in combination with other agents or methods to enhance the killing effect of codling moth pests. In actual production, chemical control is often carried out after the occurrence of pests, while attractants can control pests before boring, reducing the time and amount of pests that harm fruit production.

[0005] Therefore, it is necessary to provide an attractant core containing a codling moth larvae attractant composition to alleviate the problems of short prevention and control window period, high cost, difficult operation and difficulty in popularization, and provide diversified options for codling moth prevention and control technology. Summary of the invention

[0006] The purpose of the present invention is to overcome the defects of traditional agronomic trapping technology that it is impossible to carry out centralized prevention and control of codling moth larvae efficiently, has high labor costs and is difficult to popularize, and to provide a codling moth larvae attractant composition and its application to reduce labor cost investment, improve the effect of the agent, and achieve centralized killing, thereby helping to reduce both crops and fruits and reduce field production costs.

[0007] The first object of the present invention is to provide an attractant composition for codling moth larvae, which comprises the following components in parts by weight: 0.02 to 0.07 parts of isoquercetin, 0.6 to 0.9 parts of phloridzin, 0.01 to 0.02 parts of cyanidin-3-O-galactoside, and 0.05 to 0.12 parts of delphinidin-3-O-galactose.

[0008] Preferably, the attractant composition comprises the following components in parts by weight: the attractant composition comprises the following components in parts by weight: 0.056 parts of isoquercetin, 0.84 parts of phloridzin, 0.016 parts of cyanidin-3-O-galactoside and 0.084 parts of delphinidin-3-O-galactose.

[0009] The second object of the present invention is to provide the use of the above-mentioned attractant composition for codling moth larvae in attracting and / or controlling codling moth.

[0010] Preferably, the application includes applying the attractant composition for codling moth larvae to a blank bait core to lure codling moth larvae; or applying the attractant composition for codling moth larvae to a blank bait core, and then using the bait core in combination with a chemical pesticide / biological pesticide / sticky board.

[0011] The third object of the present invention is to provide a method for controlling codling moth larvae, which comprises applying the above-mentioned attractant composition for codling moth larvae to the habitat of codling moth to attract codling moth.

[0012] Preferably, the dosage of the attractant composition for codling moth larvae is 1 to 10 mg / m2.

[0013] Compared with the prior art, the advantages of the present invention are:

[0014] (1) Simple and flexible usage. The attractant composition for codling moth larvae provided by the present invention has multiple components that are used together in a ratio to regulate the behavior of the larvae, or combined with pesticides / sticky boards for centralized killing for prevention and control, which has stronger autonomy.

[0015] (2) Compared with traditional sex attractants and other attractants for codling moth adults, the use of bait cores containing codling moth larvae attractants is more targeted and effective in the field, significantly reducing the manpower required for prevention and control.

[0016] (3) The attractant provided by the present invention has low dosage and low price, which can reduce the application cost of behavior regulation technology and is suitable for popularization and promotion. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is the test data of Example 1. DETAILED DESCRIPTION

[0018] The present invention is further described in detail below in conjunction with specific embodiments. The given examples are only for illustrating the present invention, but not for limiting the scope of the present invention.

[0019] Example 1

[0020] From January 5 to 8, 2025, an indoor feeding induction effect test of attractants for codling moth larvae was conducted at the Institute of Plant Protection, Chinese Academy of Agricultural Sciences.

[0021] The components of the attractant: isoquercetin, phloridzin, cyanidin-3-O-galactoside, delphinidin-3-O-galactose, all have a purity of more than 99%.

[0022] Using ultrapure water and dimethyl sulfoxide in a volume ratio of 1:1 as solvent, prepare isoquercetin, phloridzin, cyanidin-3-O-galactoside, and delphinidin-3-O-galactose solutions at a concentration of 1 mg / ml, and drop 10 μl on a 0.5 cm x 0.5 cm square cellulose acetate film, and after drying at room temperature, obtain the attractant single-component attractant core. The prepared attractant single-component attractant core is sealed in aluminum foil and stored at -20°C.

[0023] According to the composition in Table 1, each component was mixed and dissolved in ultrapure water and dimethyl sulfoxide 1:1 v / v solvent to prepare a multi-component synergist aqueous solution, the total concentration of the solute in the solution was 1 mg / ml, and 10 μl was dropped on a square cellulose acetate film of 0.5 cm x 0.5 cm, and the attractant core was prepared after drying at room temperature. The prepared multi-component attractant core was sealed in aluminum foil and stored at -20°C.

[0024] Prepare the solvent with ultrapure water and dimethyl sulfoxide in a ratio of 1:1, drop 10 μL on a 0.5 cm x 0.5 cm square cellulose acetate membrane, and dry it at room temperature to obtain the control lure. The control lure is sealed in aluminum foil and stored at -20°C.

[0025] Prepare a 24-well plate, place a 0.5cm x 0.5cm x 0.5cm feed (formula see Table 2) in the middle of a well, place an attractant lure, a single-component lure or a control lure on top, select a 5th-instar codling moth larva and place it on the edge of the well, set 15 larvae for each lure in this way, cover the lid, time for 3 hours, record the weight of the feed before and after the experiment, and use the difference in the weight of the feed measured twice to represent the food intake (mg) of a single larva. The test results are shown in Table 3.

[0026] In the test data of Table 3, groups S-1 to S-4 show the effects of using lures made with a single compound; group CK shows the results of solvent control lures; and group M shows the effects of self-prepared multi-component attractant lures for codling moth larvae. As can be seen from the table, after using the multi-component attractant lures containing codling moth larvae of this embodiment, the average food intake of codling moth larvae reached 20.13 mg of feed, the average food intake of larvae treated with the single-component attractant lures was 10.73-13.47 mg, and the average food intake of larvae in the solvent control group was 12.80 mg. The multi-component attractant in this embodiment induced larvae to eat 57.26% more food than the solvent control. After statistics, Figure 1 It can be seen that after the multi-component attractant formula bait core treatment in this embodiment, the feeding amount of the codling moth larvae on the feed was significantly higher than that of the solvent control and the single-component bait core (one-way analysis of variance, F 5,84 =5.63,P=0.0002).

[0027] Table 1 Composition of multi-component attractants

[0028] Element Molecular weight Molecular formula Standard sample concentration, % Weight Isoquercetin 464.4 <![CDATA[C 21 H 20 O 12 ]]> 100 0.056 Phlorizin 436.4 <![CDATA[C 21 H 24 O 10 ]]> 100 0.84 Cyanidin-3-O-galactoside 449.4 <![CDATA[C 21 H 21 ClO 11 ]]> 100 0.016 Delphinidin-3-O-galactose 465.4 <![CDATA[C 21 H 21 O 12 ]]> 100 0.084

[0029] Table 2 Feed formula used in the test

[0030]

[0031] Table 3 Test data

[0032]

[0033]

[0034] The above are only preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be regarded as limiting the present invention, and the protection scope of the present invention should be based on the scope defined by the claims. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the spirit and scope of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A feeding attractant composition for codling moth larvae, characterized in that: The invention comprises the following components in parts by weight: 0.02-0.07 parts of isoquercetin, 0.6-0.9 parts of phlorizin, 0.01-0.02 parts of cyanidin-3-O-galactoside and 0.05-0.12 parts of delphinidin-3-O-galactose.

2. The appetite attractant composition according to claim 1, characterized in that The attractant composition comprises the following components in parts by weight: 0.056 parts of isoquercetin, 0.84 parts of phloridzin, 0.016 parts of cyanidin-3-O-galactoside and 0.084 parts of delphinidin-3-O-galactose.

3. Use of the attractant composition for codling moth larvae according to claim 1 or 2 in attracting and / or controlling codling moth.

4. The use according to claim 3, characterized in that: The application is to apply the attractant composition for codling moth larvae to a blank bait core to lure codling moth larvae; or to apply the attractant composition for codling moth larvae to a blank bait core, and then use the bait core in combination with chemical pesticides / biological pesticides.

5. A method for controlling codling moth larvae, characterized in that: The method comprises applying the attractant composition for codling moth larvae according to claim 1 or 2 to the habitat of codling moth to attract codling moth.

6. The method according to claim 5, characterized in that The dosage of the attractant composition for codling moth larvae is 1 to 10 mg / m2.

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