Plant stimulant and preparation method thereof

Abstract

The invention relates to the technical field of plant stimulants, in particular to a plant stimulant and a preparation method thereof. According to the technical scheme, the organic fertilizer is prepared from the following raw materials in parts by weight: 8 to 12 parts of glycine, 6 to 10 parts of proline, 6 to 10 parts of glutamic acid, 5 to 8 parts of protease, 4 to 6 parts of amylase, 3 to 5 parts of cellulase, 1.2 to 2.2 parts of glycerol, 1.2 to 2.2 parts of sorbitol, 8 to 12 parts of humic acid, 3 to 6 parts of fulvic acid, 4 to 8 parts of modified chitosan, 3 to 5 parts of bacillus subtilis, 2 to 4 parts of bacillus licheniformis and 2 to 4 parts of photosynthetic bacteria. According to the plant stimulant, various functional components such as amino acids, enzymes and microbial agents are reasonably matched, key components are modified, the defects that an existing plant stimulant is single in formula and insufficient in stability and compatibility are overcome, plant growth can be comprehensively promoted, the stress resistance can be comprehensively improved, and a high-quality, efficient and environment-friendly product is provided for agricultural production.

Description

Technical Field

[0001] The present invention relates to the technical field of plant stimulants, and in particular to a plant stimulant and a preparation method thereof. Background Art

[0002] In agricultural production, the healthy growth of plants is closely related to yield and quality. However, plants face many challenges during their growth, such as unbalanced nutrition, pests and diseases, and adverse environmental stress.

[0003] Traditional agricultural production often relies too much on chemical fertilizers and pesticides. Although the long-term and large-scale use of chemical fertilizers can meet the nutrient needs of plants to a certain extent, it can easily lead to soil compaction, reduced fertility, damage the ecological balance of the soil, and pollute the environment. At the same time, the abuse of pesticides can also lead to problems such as increased resistance of pests and excessive pesticide residues in agricultural products, threatening human health and ecological security.

[0004] As a new type of agricultural input, plant stimulants have unique advantages in promoting plant growth, improving plant stress resistance, and improving the quality of agricultural products, and have gradually attracted widespread attention. There are many types of existing plant stimulants, but there are still some limitations. For example, the formula of plant stimulants is single and can only play a role in one or several aspects of plant growth, and cannot fully meet the needs of plants at different growth stages and under different environmental conditions.

[0005] Some plant stimulants based on a single ingredient, such as products containing only plant growth regulators, can regulate plant growth in the short term, but long-term use may lead to imbalanced plant growth and have limited effect on improving plant resistance. For stimulants based on microbial agents, when the soil environment is not suitable, the activity of microorganisms will be inhibited and they cannot fully play their role in promoting plant growth.

[0006] In addition, most plant stimulants are deficient in stability and compatibility. During storage, the active ingredients are easily decomposed or their activity is reduced due to environmental factors. Moreover, when mixed with other agricultural inputs (such as fertilizers and pesticides), chemical reactions may occur, reducing the effects of each other and limiting their scope of application in actual production.

[0007] In order to overcome these defects of existing plant stimulants, it is of great practical significance to develop a new type of plant stimulant with multiple ingredients, stable performance, good compatibility and comprehensive plant growth promotion. This program aims to improve the stability and effectiveness of the product by rationally matching multiple functional ingredients, such as amino acids, enzymes, microbial agents, plant growth regulators, etc., and modifying key ingredients, so as to provide better quality and more efficient plant stimulant products for agricultural production.

[0008] In summary, the present application proposes a plant stimulant and a preparation method thereof. Summary of the invention

[0009] The purpose of the present invention is to address the problem in the background technology that plant stimulants cannot fully meet the needs of plants in different growth stages and under different environmental conditions, and to provide a plant stimulant and a preparation method thereof.

[0010] On the one hand, the present application proposes a plant stimulant, comprising the following raw materials in parts by weight: glycine: 8-12 parts, proline: 6-10 parts, glutamic acid: 6-10 parts, protease: 5-8 parts, amylase: 4-6 parts, cellulase: 3-5 parts, glycerol: 1.2-2.2 parts, sorbitol: 1.2-2.2 parts, humic acid: 8-12 parts, fulvic acid: 3-6 parts, modified chitosan: 4-8 parts, Bacillus subtilis: 3-5 parts, Bacillus licheniformis: 2-4 parts, photosynthetic bacteria: 2-4 parts, auxin: 1-2 parts, cytokinin: 1-2 parts, gibberellin: 0.5-1 parts, trace elements: 2-4 parts, EDTA: 0.2-0.4 parts, and active peptides: 1-2 parts.

[0011] Optionally, the specific preparation steps of the modified chitosan are as follows:

[0012] Dissolve chitosan in 1%-2% acetic acid solution to prepare a 2%-5% chitosan solution, and stir to completely dissolve it to obtain a uniform solution;

[0013] Under stirring, adding monochloroacetic acid and sodium hydroxide solution, the mass ratio of monochloroacetic acid to chitosan is between 1:1 and 3:1, and the sodium hydroxide is used to provide an alkaline environment to promote the reaction;

[0014] The reaction system is placed in a constant temperature water bath, the temperature is controlled at 50-70°C, the reaction time is about 3-6 hours, and continuous stirring is performed during the reaction to ensure uniform reaction;

[0015] The pH is adjusted to neutral with hydrochloric acid, and the product is placed in a dialysis bag and dialyzed in deionized water for 2-3 days to remove unreacted reagents and impurities. The dialyzed solution is then freeze-dried to obtain carboxymethyl chitosan, i.e., modified chitosan.

[0016] Optionally, 1-3 ml of 50% sodium hydroxide solution and 1-3 g of monochloroacetic acid are added per gram of chitosan.

[0017] Optionally, the trace elements are ferrous sulfate, zinc sulfate heptahydrate, manganese sulfate and copper sulfate in a weight ratio of 2:2:1:1.

[0018] Optionally, the active peptide is composed of glutathione and soybean peptide in a weight ratio of 1:1.

[0019] On the other hand, the present application provides a method for preparing the plant stimulant as described above, comprising the following steps:

[0020] S1. Amino acids and plant growth regulators: Weigh corresponding weight portions of glycine, proline, glutamic acid, auxin, cytokinin and gibberellin, place them in a clean and dry container, stir them evenly and set aside;

[0021] S2. Enzymes: Weigh protease, amylase and cellulase respectively, add glycerol and sorbitol and mix well;

[0022] S3. Microbial agents: Bacillus subtilis, Bacillus licheniformis and photosynthetic bacteria should be stored at 4-10°C and avoid prolonged exposure to high temperatures or direct sunlight;

[0023] S4, trace elements: ferrous sulfate, zinc sulfate heptahydrate, manganese sulfate, and copper sulfate were weighed in a weight ratio of 2:2:1:1, and dissolved in water to prepare a trace element solution. During the dissolution process, EDTA was added;

[0024] S5. Active peptides: weigh glutathione and soybean peptide in a ratio of 1:1, mix well and set aside;

[0025] S6, modified chitosan: using the carboxymethyl chitosan prepared as above;

[0026] S7, preliminary mixing: pour the pretreated amino acid and plant growth regulator solution into the reactor and stir evenly;

[0027] S8, adding humic acid and fulvic acid: weighing humic acid and fulvic acid according to weight, adding them into the reaction kettle, and stirring evenly;

[0028] S9, adding enzymes and microbial agents: slowly adding the enzymes and microbial agents treated with stabilizers into the reactor, and continue stirring. The stirring speed is adjusted to 30-50 rpm, and the stirring time is 1-2 hours;

[0029] S10, adding trace element solution: slowly dripping the trace element solution containing chelating agent into the reaction kettle, stirring while dripping, so that the trace elements are evenly dispersed in the system, and the stirring speed is maintained at 30-50 rpm;

[0030] S11, adding active peptides and modified chitosan: finally, adding active peptides and carboxymethyl chitosan into the reactor, and continuing stirring for 30-60 minutes to fully mix all the raw materials, and then dispensing the mixed plant stimulant product into containers.

[0031] Optionally, in S2, the enzymes are stored at 4-10°C for future use.

[0032] Optionally, in S4, the concentration of the trace element solution is 0.3-0.7 mol / L.

[0033] Optionally, in S8, the stirring speed is controlled at 50-100 rpm, and the stirring time is about 30-60 minutes.

[0034] Optionally, in S11, the container is an opaque plastic or glass bottle.

[0035] Compared with the prior art, the present invention has at least one of the following beneficial technical effects:

[0036] The formula of the present invention contains multiple ingredients such as amino acids, enzymes, microbial agents, plant growth regulators, etc. Amino acids can improve crop malnutrition and yellowing, and provide basic nutrition for plant growth; enzymes promote nutrient absorption and conversion, and accelerate plant physiological reactions; microbial agents regulate the soil environment and enhance plant disease resistance; plant growth regulators accurately regulate the plant growth process, and each ingredient performs its own duties and cooperates with each other to fully meet the needs of plants at different growth stages.

[0037] By modifying chitosan, its stability and compatibility in the formula are improved. At the same time, adding stabilizers such as glycerol and sorbitol to enzymes reduces the sensitivity of enzymes to environmental factors such as temperature and humidity, reduces the decomposition and activity reduction of active ingredients during storage and transportation, and ensures that the product maintains good performance during its shelf life.

[0038] Microbial agents and ingredients such as humic acid and fulvic acid can improve soil structure, increase soil fertility, and reduce the use of chemical fertilizers; while ingredients such as chitosan have certain antibacterial effects, which can reduce the occurrence of diseases and pests to a certain extent, reduce the frequency of pesticide use, and are beneficial to environmental protection and sustainable agricultural development.

[0039] The present invention overcomes the defects of the existing plant stimulants, such as single formula, insufficient stability and compatibility, by rationally combining multiple functional ingredients such as amino acids, enzymes, and microbial agents and modifying key ingredients. It can comprehensively promote plant growth and enhance stress resistance, and provide high-quality, efficient and environmentally friendly products for agricultural production. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 The present invention is a flow chart of a method for preparing a plant stimulant. DETAILED DESCRIPTION

[0041] The technical solution of the present invention is further described below in conjunction with the accompanying drawings and specific embodiments.

[0042] Example 1

[0043] The plant stimulant proposed in this embodiment includes the following raw materials in parts by weight: glycine: 8 parts, proline: 6 parts, glutamic acid: 6 parts, protease: 5 parts, amylase: 4 parts, cellulase: 3 parts, glycerol: 1.2 parts, sorbitol: 1.2 parts, humic acid: 8 parts, fulvic acid: 3 parts, modified chitosan: 4 parts, Bacillus subtilis: 3 parts, Bacillus licheniformis: 2 parts, photosynthetic bacteria: 2 parts, auxin: 1 part, cytokinin: 1 part, gibberellin: 0.5 parts, trace elements: 2 parts, EDTA: 0.2 parts, and active peptides: 1 part.

[0044] The trace elements are ferrous sulfate, zinc sulfate heptahydrate, manganese sulfate, and copper sulfate in a weight ratio of 2:2:1:1;

[0045] The active peptides are composed of glutathione and soybean peptides in a weight ratio of 1:1.

[0046] like Figure 1 , a method for preparing a phytostimulant comprises the following steps:

[0047] S1. Amino acids and plant growth regulators: Weigh corresponding weight portions of glycine, proline, glutamic acid, auxin, cytokinin and gibberellin, place them in a clean and dry container, stir them evenly and set aside;

[0048] S2. Enzymes: Weigh protease, amylase and cellulase respectively, add glycerol and sorbitol to mix evenly, and store the enzymes at 4-10°C for later use;

[0049] S3. Microbial agents: Bacillus subtilis, Bacillus licheniformis and photosynthetic bacteria should be stored at 4°C and avoid prolonged exposure to high temperatures or direct sunlight;

[0050] S4. Trace elements: Weigh ferrous sulfate, zinc sulfate heptahydrate, manganese sulfate, and copper sulfate, and dissolve them in water to prepare a trace element solution. The concentration of the trace element solution is 0.3 mol / L. During the dissolution process, add EDTA.

[0051] S5. Active peptides: weigh glutathione and soybean peptide in a ratio of 1:1, mix well and set aside;

[0052] S6, preparation of modified chitosan, the steps are as follows:

[0053] Dissolve chitosan in 1% acetic acid solution to prepare a 2% chitosan solution, and stir to completely dissolve it to obtain a uniform solution;

[0054] Under stirring, add monochloroacetic acid and sodium hydroxide solution, the mass ratio of monochloroacetic acid to chitosan is 1:1, sodium hydroxide is used to provide an alkaline environment, and 1 ml of 50% sodium hydroxide solution and 1 gram of monochloroacetic acid are added per gram of chitosan to promote the reaction;

[0055] The reaction system was placed in a constant temperature water bath, the temperature was controlled at 50°C, the reaction time was about 3 hours, and continuous stirring was performed during the reaction to ensure uniform reaction;

[0056] The pH is adjusted to neutral with hydrochloric acid, and the product is placed in a dialysis bag and dialyzed in deionized water for 2 days to remove unreacted reagents and impurities, and then the dialyzed solution is freeze-dried to obtain carboxymethyl chitosan, which is modified chitosan;

[0057] S7, preliminary mixing: pour the pretreated amino acid and plant growth regulator solution into the reactor and stir evenly;

[0058] S8, adding humic acid and fulvic acid: weighing humic acid and fulvic acid according to weight, adding them into the reaction kettle, stirring evenly, controlling the stirring speed at 50 rpm, and stirring time for about 30 minutes;

[0059] S9, adding enzymes and microbial agents: slowly adding the enzymes and microbial agents treated with stabilizers into the reactor, and continuing to stir. The stirring speed is adjusted to 30 rpm and the stirring time is 1 hour.

[0060] S10, adding trace element solution: slowly dripping the trace element solution containing chelating agent into the reaction kettle, stirring while dripping, so that the trace elements are evenly dispersed in the system, and the stirring speed is maintained at 30 rpm;

[0061] S11, adding active peptides and modified chitosan: finally, adding active peptides and carboxymethyl chitosan into the reactor, continuing stirring for 30 minutes to fully mix all the raw materials, and then packaging the mixed plant stimulant product into opaque plastic bottles.

[0062] Example 2

[0063] The plant stimulant proposed in this embodiment includes the following raw materials in parts by weight: glycine: 9 parts, proline: 7 parts, glutamic acid: 7 parts, protease: 6 parts, amylase: 4.5 parts, cellulase: 3.5 parts, glycerol: 1.5 parts, sorbitol: 1.5 parts, humic acid: 9 parts, fulvic acid: 4 parts, modified chitosan: 5 parts, Bacillus subtilis: 3.5 parts, Bacillus licheniformis: 2.5 parts, photosynthetic bacteria: 2.5 parts, auxin: 1.25 parts, cytokinin: 1.25 parts, gibberellin: 0.625 parts, trace elements: 2.5 parts, EDTA: 0.25 parts, and active peptides: 1.25 parts.

[0064] The trace elements are ferrous sulfate, zinc sulfate heptahydrate, manganese sulfate, and copper sulfate in a weight ratio of 2:2:1:1;

[0065] The active peptides are composed of glutathione and soybean peptides in a weight ratio of 1:1.

[0066] The preparation method of plant stimulant comprises the following steps:

[0067] S1. Amino acids and plant growth regulators: Weigh corresponding weight portions of glycine, proline, glutamic acid, auxin, cytokinin and gibberellin, place them in a clean and dry container, stir them evenly and set aside;

[0068] S2. Enzymes: Weigh protease, amylase and cellulase respectively, add glycerol and sorbitol to mix evenly, and store the enzymes at 5°C for later use;

[0069] S3. Microbial agents: Bacillus subtilis, Bacillus licheniformis and photosynthetic bacteria should be stored at 5°C and avoid prolonged exposure to high temperatures or direct sunlight;

[0070] S4. Trace elements: Weigh ferrous sulfate, zinc sulfate heptahydrate, manganese sulfate, and copper sulfate, and dissolve them in water to prepare a trace element solution. The concentration of the trace element solution is 0.4 mol / L. During the dissolution process, add EDTA.

[0071] S5. Active peptides: weigh glutathione and soybean peptide in a ratio of 1:1, mix well and set aside;

[0072] S6, preparation of modified chitosan, the steps are as follows:

[0073] Dissolve chitosan in 1.2% acetic acid solution to prepare a 3% chitosan solution, and stir to completely dissolve it to obtain a uniform solution;

[0074] Under stirring, add monochloroacetic acid and sodium hydroxide solution, the mass ratio of monochloroacetic acid to chitosan is between 1.5:1, sodium hydroxide is used to provide an alkaline environment, and 1.5 ml of 50% sodium hydroxide solution and 1.5 g of monochloroacetic acid are added per gram of chitosan to promote the reaction;

[0075] The reaction system was placed in a constant temperature water bath, the temperature was controlled at 55°C, the reaction time was about 4 hours, and continuous stirring was performed during the reaction to ensure uniform reaction;

[0076] The pH is adjusted to neutral with hydrochloric acid, and the product is placed in a dialysis bag and dialyzed in deionized water for 2.5 days to remove unreacted reagents and impurities, and then the dialyzed solution is freeze-dried to obtain carboxymethyl chitosan, which is modified chitosan;

[0077] S7, preliminary mixing: pour the pretreated amino acid and plant growth regulator solution into the reactor and stir evenly;

[0078] S8, adding humic acid and fulvic acid: weighing humic acid and fulvic acid according to weight, adding them into the reaction kettle, stirring evenly, controlling the stirring speed at 60 rpm, and stirring time for about 40 minutes;

[0079] S9, adding enzymes and microbial agents: slowly adding the enzymes and microbial agents treated with stabilizers into the reactor, and continuing to stir. The stirring speed is adjusted to 35 rpm and the stirring time is 1.5 hours.

[0080] S10, adding trace element solution: slowly dripping the trace element solution containing chelating agent into the reaction kettle, stirring while dripping, so that the trace elements are evenly dispersed in the system, and the stirring speed is maintained at 35 rpm;

[0081] S11, adding active peptides and modified chitosan: finally, adding active peptides and carboxymethyl chitosan into the reactor, continuing stirring for 40 minutes to fully mix all the raw materials, and then packaging the mixed plant stimulant product into opaque glass bottles.

[0082] Example 3

[0083] The plant stimulant proposed in this embodiment includes the following raw materials in parts by weight: glycine: 10 parts, proline: 8 parts, glutamic acid: 8 parts, protease: 6.5 parts, amylase: 5 parts, cellulase: 4 parts, glycerol: 1.7 parts, sorbitol: 1.7 parts, humic acid: 10 parts, fulvic acid: 4.5 parts, modified chitosan: 6 parts, Bacillus subtilis: 4 parts, Bacillus licheniformis: 3 parts, photosynthetic bacteria: 3 parts, auxin: 1.5 parts, cytokinin: 1.5 parts, gibberellin: 0.75 parts, trace elements: 3 parts, EDTA: 0.3 parts, and active peptides: 1.5 parts.

[0084] Trace elements include ferrous sulfate: 1.2 parts, zinc sulfate heptahydrate: 1.2 parts, manganese sulfate: 0.3 parts, copper sulfate: 0.3 parts; active peptides include glutathione: 0.75 parts, soy peptide: 0.75 parts;

[0085] The trace elements are ferrous sulfate, zinc sulfate heptahydrate, manganese sulfate, and copper sulfate in a weight ratio of 2:2:1:1;

[0086] The active peptides are composed of glutathione and soybean peptides in a weight ratio of 1:1.

[0087] The preparation method of plant stimulant comprises the following steps:

[0088] S1. Amino acids and plant growth regulators: Weigh corresponding weight portions of glycine, proline, glutamic acid, auxin, cytokinin and gibberellin, place them in a clean and dry container, stir them evenly and set aside;

[0089] S2. Enzymes: Weigh protease, amylase and cellulase respectively, add glycerol and sorbitol to mix evenly, and store the enzymes at 7°C for later use;

[0090] S3. Microbial agents: Bacillus subtilis, Bacillus licheniformis and photosynthetic bacteria should be stored at 7°C and avoid prolonged exposure to high temperatures or direct sunlight;

[0091] S4. Trace elements: Weigh ferrous sulfate, zinc sulfate heptahydrate, manganese sulfate, and copper sulfate, and dissolve them in water to prepare a trace element solution. The concentration of the trace element solution is 0.5 mol / L. During the dissolution process, add EDTA.

[0092] S5. Active peptides: weigh glutathione and soybean peptide in a ratio of 1:1, mix well and set aside;

[0093] S6, preparation of modified chitosan, the steps are as follows:

[0094] Dissolve chitosan in 1.5% acetic acid solution to prepare a 4% chitosan solution, and stir to completely dissolve it to obtain a uniform solution;

[0095] Under stirring, add monochloroacetic acid and sodium hydroxide solution, the mass ratio of monochloroacetic acid to chitosan is 2:1, sodium hydroxide is used to provide an alkaline environment, and 2 ml of 50% sodium hydroxide solution and 2 grams of monochloroacetic acid are added per gram of chitosan to promote the reaction;

[0096] The reaction system was placed in a constant temperature water bath, the temperature was controlled at 60°C, the reaction time was about 4.5 hours, and continuous stirring was performed during the reaction to ensure uniform reaction;

[0097] The pH is adjusted to neutral with hydrochloric acid, and the product is placed in a dialysis bag and dialyzed in deionized water for 2.5 days to remove unreacted reagents and impurities, and then the dialyzed solution is freeze-dried to obtain carboxymethyl chitosan, which is modified chitosan;

[0098] S7, preliminary mixing: pour the pretreated amino acid and plant growth regulator solution into the reactor and stir evenly;

[0099] S8, adding humic acid and fulvic acid: weighing humic acid and fulvic acid according to weight, adding them into the reaction kettle, stirring evenly, controlling the stirring speed at 75 rpm, and stirring time for about 45 minutes;

[0100] S9, adding enzymes and microbial agents: slowly adding the enzymes and microbial agents treated with stabilizers into the reactor, and continuing to stir. The stirring speed is adjusted to 40 rpm and the stirring time is 1.5 hours.

[0101] S10, adding trace element solution: slowly dripping the trace element solution containing chelating agent into the reaction kettle, stirring while dripping, so that the trace elements are evenly dispersed in the system, and the stirring speed is maintained at 40 rpm;

[0102] S11, adding active peptides and modified chitosan: finally, adding active peptides and carboxymethyl chitosan into the reactor, and continuing stirring for 45 minutes to fully mix all the raw materials, and then packaging the mixed plant stimulant product into opaque plastic.

[0103] Example 4

[0104] The plant stimulant proposed in this embodiment includes the following raw materials in parts by weight: glycine: 11 parts, proline: 9 parts, glutamic acid: 9 parts, protease: 7 parts, amylase: 5.5 parts, cellulase: 4.5 parts, glycerol: 2 parts, sorbitol: 2 parts, humic acid: 11 parts, fulvic acid: 5 parts, modified chitosan: 7 parts, Bacillus subtilis: 4.5 parts, Bacillus licheniformis: 3.5 parts, photosynthetic bacteria: 3.5 parts, auxin: 1.75 parts, cytokinin: 1.75 parts, gibberellin: 0.875 parts, trace elements: 3.5 parts, EDTA: 0.35 parts, and active peptides: 1.75 parts.

[0105] The trace elements are ferrous sulfate, zinc sulfate heptahydrate, manganese sulfate, and copper sulfate in a weight ratio of 2:2:1:1;

[0106] The active peptides are composed of glutathione and soybean peptides in a weight ratio of 1:1.

[0107] The preparation method of plant stimulant comprises the following steps:

[0108] S1. Amino acids and plant growth regulators: Weigh corresponding weight portions of glycine, proline, glutamic acid, auxin, cytokinin and gibberellin, place them in a clean and dry container, stir them evenly and set aside;

[0109] S2. Enzymes: Weigh protease, amylase and cellulase respectively, add glycerol and sorbitol to mix evenly, and store the enzymes at 8°C for later use;

[0110] S3. Microbial agents: Bacillus subtilis, Bacillus licheniformis and photosynthetic bacteria should be stored at 9°C and avoid prolonged exposure to high temperatures or direct sunlight;

[0111] S4. Trace elements: Weigh ferrous sulfate, zinc sulfate heptahydrate, manganese sulfate, and copper sulfate, and dissolve them in water to prepare a trace element solution. The concentration of the trace element solution is 0.6 mol / L. During the dissolution process, add EDTA.

[0112] S5. Active peptides: weigh glutathione and soybean peptide in a ratio of 1:1, mix well and set aside;

[0113] S6, preparation of modified chitosan, the steps are as follows:

[0114] Dissolve chitosan in 1.8% acetic acid solution to prepare a 4% chitosan solution, and stir to completely dissolve it to obtain a uniform solution;

[0115] Under stirring, add monochloroacetic acid and sodium hydroxide solution, the mass ratio of monochloroacetic acid to chitosan is 2.5:1, sodium hydroxide is used to provide an alkaline environment, and 2.5 ml of 50% sodium hydroxide solution and 2.5 g of monochloroacetic acid are added per gram of chitosan to promote the reaction;

[0116] The reaction system was placed in a constant temperature water bath, the temperature was controlled at 65°C, the reaction time was about 5 hours, and continuous stirring was performed during the reaction to ensure uniform reaction;

[0117] The pH is adjusted to neutral with hydrochloric acid, and the product is placed in a dialysis bag and dialyzed in deionized water for 2.5 days to remove unreacted reagents and impurities, and then the dialyzed solution is freeze-dried to obtain carboxymethyl chitosan, which is modified chitosan;

[0118] S7, preliminary mixing: pour the pretreated amino acid and plant growth regulator solution into the reactor and stir evenly;

[0119] S8, adding humic acid and fulvic acid: weighing humic acid and fulvic acid according to weight, adding them into the reaction kettle, stirring evenly, controlling the stirring speed at 80 rpm, and stirring time for about 55 minutes;

[0120] S9, adding enzymes and microbial agents: slowly adding the enzymes and microbial agents treated with stabilizers into the reactor, and continuing to stir. The stirring speed is adjusted to 48 rpm, and the stirring time is 1.8 hours;

[0121] S10, adding trace element solution: slowly dripping the trace element solution containing chelating agent into the reaction kettle, stirring while dripping, so that the trace elements are evenly dispersed in the system, and the stirring speed is maintained at 45 rpm;

[0122] S11, adding active peptides and modified chitosan: Finally, add active peptides and carboxymethyl chitosan into the reactor, continue stirring for 55 minutes to fully mix all the raw materials, and then pack the mixed plant stimulant product into opaque plastic or glass bottles.

[0123] Example 5

[0124] The plant stimulant proposed in this embodiment includes the following raw materials in parts by weight: glycine: 12 parts, proline: 10 parts, glutamic acid: 10 parts, protease: 8 parts, amylase: 6 parts, cellulase: 5 parts, glycerol: 2.2 parts, sorbitol: 2.2 parts, humic acid: 12 parts, fulvic acid: 6 parts, modified chitosan: 8 parts, Bacillus subtilis: 5 parts, Bacillus licheniformis: 4 parts, photosynthetic bacteria: 4 parts, auxin: 2 parts, cytokinin: 2 parts, gibberellin: 1 part, trace elements: 4 parts, EDTA: 0.4 parts, and active peptides: 2 parts.

[0125] The trace elements are ferrous sulfate, zinc sulfate heptahydrate, manganese sulfate, and copper sulfate in a weight ratio of 2:2:1:1;

[0126] The active peptides are composed of glutathione and soybean peptides in a weight ratio of 1:1.

[0127] The preparation method of plant stimulant comprises the following steps:

[0128] S1. Amino acids and plant growth regulators: Weigh corresponding weight portions of glycine, proline, glutamic acid, auxin, cytokinin and gibberellin, place them in a clean and dry container, stir them evenly and set aside;

[0129] S2. Enzymes: Weigh protease, amylase and cellulase respectively, add glycerol and sorbitol to mix evenly, and store the enzymes at 10°C for later use;

[0130] S3. Microbial agents: Bacillus subtilis, Bacillus licheniformis and photosynthetic bacteria should be stored at 10°C and avoid prolonged exposure to high temperatures or direct sunlight;

[0131] S4. Trace elements: Weigh ferrous sulfate, zinc sulfate heptahydrate, manganese sulfate, and copper sulfate, and dissolve them in water to prepare a trace element solution. The concentration of the trace element solution is 0.7 mol / L. During the dissolution process, add EDTA.

[0132] S5. Active peptides: weigh glutathione and soybean peptide in a ratio of 1:1, mix well and set aside;

[0133] S6, preparation of modified chitosan, the steps are as follows:

[0134] Dissolve chitosan in 2% acetic acid solution to prepare a 5% chitosan solution, and stir to completely dissolve it to obtain a uniform solution;

[0135] Under stirring, add monochloroacetic acid and sodium hydroxide solution, the mass ratio of monochloroacetic acid to chitosan is 3:1, sodium hydroxide is used to provide an alkaline environment, 3 ml of 50% sodium hydroxide solution and 3 grams of monochloroacetic acid are added per gram of chitosan to promote the reaction;

[0136] The reaction system was placed in a constant temperature water bath, the temperature was controlled at 70°C, the reaction time was about 6 hours, and continuous stirring was performed during the reaction to ensure uniform reaction;

[0137] The pH is adjusted to neutral with hydrochloric acid, and the product is placed in a dialysis bag and dialyzed in deionized water for 3 days to remove unreacted reagents and impurities, and then the dialyzed solution is freeze-dried to obtain carboxymethyl chitosan, which is modified chitosan;

[0138] S7, preliminary mixing: pour the pretreated amino acid and plant growth regulator solution into the reactor and stir evenly;

[0139] S8, adding humic acid and fulvic acid: weighing humic acid and fulvic acid according to weight, adding them into the reaction kettle, stirring evenly, controlling the stirring speed at 100 rpm, and stirring time for about 60 minutes;

[0140] S9, adding enzymes and microbial agents: slowly adding the enzymes and microbial agents treated with stabilizers into the reactor, and continuing to stir. The stirring speed is adjusted to 50 rpm and the stirring time is 2 hours.

[0141] S10, adding trace element solution: slowly dripping the trace element solution containing chelating agent into the reaction kettle, stirring while dripping, so that the trace elements are evenly dispersed in the system, and the stirring speed is maintained at 50 rpm;

[0142] S11, adding active peptides and modified chitosan: Finally, add active peptides and carboxymethyl chitosan into the reactor, continue stirring for 60 minutes to fully mix all the raw materials, and then pack the mixed plant stimulant product into opaque plastic or glass bottles.

[0143] The formula of the present invention contains multiple ingredients such as amino acids, enzymes, microbial agents, plant growth regulators, etc. Amino acids can improve crop malnutrition and yellowing, and provide basic nutrition for plant growth; enzymes promote nutrient absorption and conversion, and accelerate plant physiological reactions; microbial agents regulate the soil environment and enhance plant disease resistance; plant growth regulators accurately regulate the plant growth process, and each ingredient performs its own duties and cooperates with each other to fully meet the needs of plants at different growth stages.

[0144] By modifying chitosan, its stability and compatibility in the formula are improved. At the same time, adding stabilizers such as glycerol and sorbitol to enzymes reduces the sensitivity of enzymes to environmental factors such as temperature and humidity, reduces the decomposition and activity reduction of active ingredients during storage and transportation, and ensures that the product maintains good performance during its shelf life.

[0145] Reduced over-reliance on chemical fertilizers and pesticides. Microbial agents and ingredients such as humic acid and fulvic acid can improve soil structure, increase soil fertility, and reduce the use of chemical fertilizers; while ingredients such as chitosan have certain antibacterial effects, which can reduce the occurrence of pests and diseases to a certain extent, reduce the frequency of pesticide use, and are beneficial to environmental protection and sustainable agricultural development.

[0146] In the formula of the present invention, EDTA combines with trace elements to form chelates, which not only reduces the influence of trace elements on enzyme activity, but also makes the trace elements in chelated form easier to be absorbed and utilized in plants, and can protect the activity of enzymes to a certain extent and prolong the action time of enzymes, which is an effect that is difficult to achieve when a single ingredient acts alone.

[0147] The unique effect of modified chitosan on microbial agents: The modified carboxymethyl chitosan has better water solubility and biocompatibility. It can not only play an antibacterial role and regulate plant growth, but also provide a more suitable living environment for microbial agents. It can promote the colonization and reproduction of microorganisms in the soil and enhance the adaptability of microbial agents to the soil environment.

[0148] It is worth mentioning that through the synergy of amino acids and trace elements, amino acids can form chelates with trace elements to improve the stability and absorbability of trace elements; enzymes accelerate the absorption and conversion of nutrients by plants, while humic acid and fulvic acid can improve soil structure, increase soil's ability to retain fertilizer and water, and promote root absorption of nutrients. The synergistic effect of various components greatly improves the absorption and utilization efficiency of nutrients by plants.

[0149] Among them, the joint action of amino acids such as proline, microbial agents, chitosan and plant growth regulators can enhance plant stress resistance. When facing adversities such as high temperature, drought, salinity and alkali, amino acids can regulate the osmotic pressure of plant cells, microbial agents can improve the soil environment, chitosan can induce plant resistance, and plant growth regulators can regulate plant physiological metabolism. These multiple approaches can significantly improve the plant's stress resistance.

[0150] In addition, through the reasonable proportion of auxin, cytokinin and gibberellin, the stems are promoted to be strong, leaves to grow, and lateral buds to sprout, while also stimulating the development of the root system, making the overall growth of the plant more coordinated and increasing crop yield and quality. Active peptides, trace elements and humic acid and other ingredients can help improve the quality of agricultural products. For example, it can promote fruit enlargement and color change, make the seeds fuller and taste better. Moreover, by reducing the use of fertilizers and pesticides, the harmful substances in agricultural products can be reduced, and greener and healthier agricultural products can be produced.

[0151] In the preparation process of the present invention, amino acids and plant growth regulators are preliminarily mixed, and then other ingredients are added in sequence, and different stirring speeds and times are set at different stages. This orderly mixing method, combined with appropriate stirring parameters, allows various raw materials to be fully contacted and evenly dispersed, improves the uniformity of the product, avoids the problem of excessively high or low concentration of local components caused by uneven mixing, and thus ensures stable product quality.

[0152] Chitosan is modified, and the detailed preparation steps ensure the quality of the modified chitosan. The modified chitosan is better compatible with other ingredients, reducing the instability of the product that may be caused by the interaction between the ingredients. At the same time, EDTA is added when the trace elements are dissolved to form chelates, which reduces the adverse effects of trace elements on other ingredients and further enhances the stability of the product during storage and use. The reaction temperature is controlled at 50-70°C, the reaction time is 3-6 hours, and the mass ratio of monochloroacetic acid and chitosan and the amount of sodium hydroxide solution added have clear ranges. This allows the modification reaction to proceed as expected, accurately changing the chemical structure of chitosan, thereby obtaining carboxymethyl chitosan with better performance, laying the foundation for improving the overall performance of plant stimulants.

[0153] The entire preparation method optimizes each link to make the final product more compatible. It can not only remain stable during storage, but also reduce the probability of adverse reactions when mixed with other agricultural inputs. This means that the plant stimulant can be more conveniently used in combination with fertilizers, pesticides, etc. in actual agricultural production, expanding its application scenarios and improving its efficiency.

[0154] The above specific embodiments are only several optional embodiments of the present invention. Based on the technical solutions of the present invention and the relevant inspirations of the above embodiments, those skilled in the art can make various alternative improvements and combinations to the above specific embodiments.

Claims

1. A plant stimulant, characterized in that The invention comprises the following raw materials in parts by weight: glycine: 8-12 parts, proline: 6-10 parts, glutamic acid: 6-10 parts, protease: 5-8 parts, amylase: 4-6 parts, cellulase: 3-5 parts, glycerol: 1.2-2.2 parts, sorbitol: 1.2-2.2 parts, humic acid: 8-12 parts, fulvic acid: 3-6 parts, modified chitosan: 4-8 parts, Bacillus subtilis: 3-5 parts, Bacillus licheniformis: 2-4 parts, photosynthetic bacteria: 2-4 parts, auxin: 1-2 parts, cytokinin: 1-2 parts, gibberellin: 0.5-1 parts, trace elements: 2-4 parts, EDTA: 0.2-0.4 parts, and active peptides: 1-2 parts.

2. A plant stimulant according to claim 1, characterized in that The specific preparation steps of the modified chitosan are as follows: Dissolve chitosan in 1%-2% acetic acid solution to prepare a 2%-5% chitosan solution, and stir to completely dissolve it to obtain a uniform solution; Under stirring, adding monochloroacetic acid and sodium hydroxide solution, the mass ratio of monochloroacetic acid to chitosan is between 1:1 and 3:1, and the sodium hydroxide is used to provide an alkaline environment to promote the reaction; The reaction system is placed in a constant temperature water bath, the temperature is controlled at 50-70°C, the reaction time is about 3-6 hours, and continuous stirring is performed during the reaction to ensure uniform reaction; The pH is adjusted to neutral with hydrochloric acid, and the product is placed in a dialysis bag and dialyzed in deionized water for 2-3 days to remove unreacted reagents and impurities. The dialyzed solution is then freeze-dried to obtain carboxymethyl chitosan, i.e., modified chitosan.

3. A plant stimulant according to claim 2, characterized in that: For each gram of chitosan, add 1-3 ml of 50% sodium hydroxide solution and 1-3 g of monochloroacetic acid.

4. A plant stimulant according to claim 1, characterized in that: The trace elements are ferrous sulfate, zinc sulfate heptahydrate, manganese sulfate and copper sulfate in a weight ratio of 2:2:1:

1.

5. A plant stimulant according to claim 1, characterized in that: The active peptides are composed of glutathione and soybean peptides in a weight ratio of 1:

1.

6. A method for preparing a plant stimulant according to any one of claims 1 to 5, characterized in that: The following steps are involved: S1. Amino acids and plant growth regulators: Weigh corresponding weight portions of glycine, proline, glutamic acid, auxin, cytokinin and gibberellin respectively, place in a clean and dry container, stir evenly and set aside; S2. Enzymes: Weigh protease, amylase and cellulase respectively, add glycerol and sorbitol and mix well; S3. Microbial agents: Bacillus subtilis, Bacillus licheniformis and photosynthetic bacteria should be stored at 4-10°C and avoid prolonged exposure to high temperatures or direct sunlight; S4, trace elements: ferrous sulfate, zinc sulfate heptahydrate, manganese sulfate, and copper sulfate were weighed in a weight ratio of 2:2:1:1, and dissolved in water to prepare a trace element solution. During the dissolution process, EDTA was added; S5. Active peptides: weigh glutathione and soybean peptide in a ratio of 1:1, mix well and set aside; S6, modified chitosan: using the carboxymethyl chitosan prepared as above; S7, preliminary mixing: pour the pretreated amino acid and plant growth regulator solution into the reactor and stir evenly; S8, adding humic acid and fulvic acid: weighing humic acid and fulvic acid according to weight, adding them into the reaction kettle, and stirring evenly; S9, adding enzymes and microbial agents: slowly adding the enzymes and microbial agents treated with stabilizers into the reactor, and continue stirring. The stirring speed is adjusted to 30-50 rpm, and the stirring time is 1-2 hours; S10, adding trace element solution: slowly dripping the trace element solution containing chelating agent into the reaction kettle, stirring while dripping, so that the trace elements are evenly dispersed in the system, and the stirring speed is maintained at 30-50 rpm; S11, adding active peptides and modified chitosan: finally, adding active peptides and carboxymethyl chitosan into the reactor, and continuing stirring for 30-60 minutes to fully mix all the raw materials, and then dispensing the mixed plant stimulant product into containers.

7. The method for preparing a plant stimulant according to claim 6, characterized in that: In the S2, the enzymes are stored at 4-10°C for future use.

8. The method for preparing a plant stimulant according to claim 6, characterized in that: In the S4, the concentration of the trace element solution is 0.3-0.7 mol / L.

9. The method for preparing a plant stimulant according to claim 6, characterized in that: In S8, the stirring speed is controlled at 50-100 rpm, and the stirring time is about 30-60 minutes.

10. The method for preparing a plant stimulant according to claim 6, characterized in that: In S11, the container is a light-proof plastic or glass bottle.

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