Color-changing antibacterial mouthwash as well as preparation method and application thereof

Abstract

The invention relates to the field of oral care products, and provides color-changing antibacterial mouthwash and a preparation method thereof, the color-changing antibacterial mouthwash comprises the following raw materials by mass: 1-30% of a bacteriostatic agent, 1-20% of a synergistic agent, 0.5-5% of a surfactant, 20-30% of a humectant, 0.5-3% of a preservative, 0.2-1.2% of a pH regulator, and 1-20% of a detection agent; the bacteriostatic agent comprises a composite extract and a nano composite material, and the composite extract comprises a dandelion extract, a thunbergia odorifera bark extract, a gentian extract, a bighead atractylodes rhizome extract, a licorice extract, a codonopsis pilosula extract and a coptis chinensis extract; the detection agent is hydrolyzed procyanidine. The prepared mouthwash not only can visually reflect the pH value of the oral cavity through color change, but also can effectively resist bacteria and relieve problems caused by abnormal pH value of the oral cavity.

Description

Technical Field

[0001] The invention relates to the field of oral care products, and in particular to a color-changing antibacterial mouthwash and a preparation method thereof. Background Art

[0002] In the field of oral care, mouthwash has been widely used as an important auxiliary tool. It can not only effectively clean the mouth and cover up the bad breath caused by bacteria or yeast decomposing food residues, but also bring a fresh and comfortable feeling to users. In recent years, with the intensification of market competition and the increase in consumer demand, mouthwash products are no longer limited to simple breath freshening and bad breath elimination, but pay more attention to its function of preventing and treating dental and oral diseases, aiming to comprehensively improve users' oral health.

[0003] Oral pH, as an important indicator to measure oral health, has a significant impact on oral health. Oral pH is usually expressed as pH value, and its ideal range should be between 6.6 and 7.1. When the oral pH value is low, that is, too acidic, it is easy to cause a series of oral problems, such as gingivitis, bleeding gums, caries, and bad breath. What's more serious is that excessive oral acidity may also lead to the dissolution of tooth enamel, which is a permanent damage that makes teeth brittle and easy to break. On the contrary, an abnormal increase in oral pH may also have an adverse effect on oral health.

[0004] However, the current method for detecting oral pH mainly relies on the test paper colorimetry, which is not only cumbersome to operate, but also easily interfered by various factors, resulting in inaccurate results. Given that mouthwash is a necessity for daily oral care and can fully contact the oral cavity every time it is used, it is particularly important to develop a convenient method that can use mouthwash to detect oral pH.

[0005] In addition, although there are many types of mouthwash products on the current market, most of them lack effective solutions for the problem of abnormal oral pH. In particular, for oral problems caused by oral pH imbalance, such as oral ulcers and gingivitis, the treatment effect of existing mouthwash products is limited. Therefore, the development of a mouthwash product that can not only detect oral pH, but also effectively treat and prevent abnormal oral pH has important practical significance and market value. Summary of the invention

[0006] In summary, the present invention is aimed at the above problems existing in current mouthwash products, and provides a color-changing antibacterial mouthwash and a preparation method thereof. The prepared mouthwash can not only directly reflect the pH value of the oral cavity through color changes, but also effectively resist bacteria and alleviate the problems caused by abnormal pH value of the oral cavity, providing users with a more comprehensive and convenient oral care solution.

[0007] The present invention is achieved through the following technical solutions: A color-changing antibacterial mouthwash is composed of the following raw materials in percentage by mass: 1-30% of an antibacterial agent, 1-20% of a synergistic enhancer, 0.5-5% of a surfactant, 20-30% of a moisturizing agent, 0.5-3% of a preservative, 0.2-1.2% of a pH regulator, and 1-20% of a detection agent; the antibacterial agent comprises a composite extract and a nano-composite material, the composite extract being a dandelion extract, a thick sedge bark extract, a gentian extract, a white atractylodes extract, a liquorice extract, a codonopsis extract, and a coptis extract; the detection agent is a hydrolyzed proanthocyanidin.

[0008] Furthermore, the invention is composed of the following raw materials in percentage by weight: 15-30% antibacterial agent, 10-20% synergistic enhancer, 2-5% surfactant, 25-30% moisturizing agent, 1.5-3% preservative, 0.5-1.0% pH regulator and 10-20% detection agent.

[0009] Furthermore, the invention is composed of the following raw materials in percentage by weight: 22-28% antibacterial agent, 15-18% synergistic enhancer, 2-4% surfactant, 28-30% moisturizing agent, 1.5-2.5% preservative, 0.8-1.0% pH regulator and 15-20% detection agent.

[0010] Furthermore, the invention is composed of the following raw materials in percentage by weight: 20% of antibacterial agent, 15% of synergistic enhancer, 3% of surfactant, 28% of moisturizing agent, 2.1% of preservative, 1.0% of pH adjusting agent and 15% of detection agent.

[0011] Furthermore, the composition is composed of the following raw materials in percentage by mass: 30% of antibacterial agent, 20% of synergistic enhancer, 5% of surfactant, 25% of moisturizing agent, 1.5% of preservative, 0.8% of pH regulator and 10% of detection agent.

[0012] Furthermore, the composite extract is dandelion extract, paulownia crassifolia bark extract, gentiana extract, atractylodes macrocephala extract, licorice extract, codonopsis pilosula extract, and coptis chinensis extract.

[0013] Furthermore, the extraction method of dandelion extract is: dissolving the dandelion raw material in ethanol and ether organic solvents, and extracting at 75°C, ethanol concentration of 65%, and a solid-liquid ratio of 1:30.

[0014] Dandelion is bitter, sweet and cold in nature. It has the effects of clearing away swelling, clearing away heat and detoxifying, inhibiting bacteria, and clearing away dampness and heat. It can inhibit a variety of bacteria in the mouth, including pathogenic streptococci. Dandelion extract also has anti-inflammatory properties and can reduce gingivitis and inflammatory reactions of the oral mucosa. This makes dandelion extract an effective auxiliary ingredient for improving oral inflammation caused by infection. Dandelion extract has the effect of clearing heat and detoxifying, which can relieve bad breath caused by heat and toxins in the mouth. This is also supported in traditional Chinese medicine theory, which believes that dandelion can clear heat and detoxify, thereby improving bad breath.

[0015] Furthermore, the extraction method of the Psoralea corylifolia bark extract is: using 96% ethanol as a solvent, under the action of ultrasound, the extraction is carried out with the mass ratio of Psoralea corylifolia bark powder to solvent being 1:10.

[0016] The compounds with antioxidant activity in Magnolia officinalis bark extract mainly include magnolol and honokiol. These compounds belong to the class of biphenyl lignans and have a variety of biological activities, including antioxidant, antibacterial, antidiabetic, anti-inflammatory, anti-aggregation, analgesic, anti-tumor, antiviral and neuroprotective. Magnolia officinalis bark extract can inhibit the growth of pathogenic bacteria such as oral streptococci, thereby reducing the formation of dental plaque and the occurrence of bad breath. The role of Magnolia officinalis bark extract in mouthwash is mainly to improve oral hygiene and reduce bacterial infections through its antibacterial properties, thereby achieving the effect of preventing gum disease, dental plaque and bad breath.

[0017] Furthermore, the extraction method of the gentian extract is: using methanol as an extraction solvent, extracting under the action of ultrasound, with a solid-liquid ratio of 1:40, an extraction time of 10 minutes, and an extraction temperature of 60°C.

[0018] Gentian extract has significant antibacterial properties and can inhibit the growth of many bacteria. Gentian extract has an inhibitory effect on bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. Its use in mouthwash can effectively reduce the number of bacteria in the mouth, thereby preventing oral infections and periodontal disease. Gentian extract also has anti-inflammatory effects and can reduce oral inflammation. Its active ingredients such as gentiopicroside can exert anti-inflammatory effects by regulating the levels of inflammatory factors. This property makes the use of gentian extract in mouthwash helpful in relieving oral inflammation and discomfort.

[0019] Furthermore, the extraction method of the Atractylodes macrocephala extract is: using methanol as the extraction solvent, extracting under the action of ultrasound, with a solid-liquid ratio of 1:10, an ultrasonic power of 15.5 W, and an ultrasonic time of 2.6 hours.

[0020] Atractylodes macrocephala extract has a significant inhibitory effect on the main dental caries bacteria in the oral cavity. Atractylodes macrocephala extract can effectively inhibit the growth, adhesion, and acid and sugar production of these bacteria, thereby reducing the formation of dental plaque and demineralization of tooth enamel. In addition, Atractylodes macrocephala extract has been shown to reduce the biofilm formation ability of bacteria in the oral cavity, further preventing the occurrence of dental caries. Atractylodes macrocephala extract has the effect of increasing the pH value of saliva, which helps to reduce the acidic environment in the oral cavity and thus reduce the risk of dental caries. Atractylodes macrocephala extract is rich in polysaccharides and flavonoids, which have strong antioxidant and anti-inflammatory effects. These properties enable the use of Atractylodes macrocephala extract in mouthwash to help reduce oral inflammation and protect oral health.

[0021] Furthermore, the extraction method of the licorice extract is: using 70% ethanol as a solvent, subjecting the licorice powder to ultrasonic treatment for 2 hours at 25° C., and then concentrating by vacuum evaporation to obtain a filtrate.

[0022] Licorice extract has significant antibacterial activity and can effectively inhibit the growth of bacteria in the mouth. Licorice extract has an inhibitory effect on a variety of oral bacteria such as Helicobacter pylori, Candida albicans, and Staphylococcus aureus. Its antibacterial effect is even better than some traditional mouthwash ingredients such as chlorhexidine. In addition, the active ingredients in licorice extract, such as glycyrrhetinic acid and liquiritin, can inhibit the growth and metabolism of bacteria. Licorice extract also has anti-inflammatory properties and can reduce oral inflammation. When treating oral ulcers and periodontal disease, licorice extract can relieve symptoms by inhibiting the release of inflammatory factors.

[0023] Furthermore, the extraction method of the Codonopsis pilosula extract is as follows: washing, drying, crushing and sieving the Codonopsis pilosula, mixing the Codonopsis pilosula powder with a 60% ethanol solution using an ultrasonic-assisted extraction method, and performing ultrasonic extraction at 30°C with a power of 300W for 30 minutes.

[0024] Codonopsis extract contains rich active ingredients, such as codonopsis polysaccharide and polyanthocyanin, which have the effects of improving immunity, anti-oxidation, and anti-inflammatory. Its application in mouthwash can help enhance the immunity of oral mucosa and reduce the occurrence of oral inflammation. Codonopsis extract has the function of regulating the immune system and inhibiting the proliferation of inflammatory cells, which makes its use in mouthwash effective in preventing and relieving common oral problems such as oral ulcers and gingivitis.

[0025] Furthermore, the extraction method of the Coptis chinensis extract is as follows: the Coptis chinensis powder is dried at 50°C, and the Coptis chinensis powder is extracted at an ultrasonic power of 150W for 20 minutes by an ultrasonic-assisted extraction method, followed by cooling, centrifugation and filtration.

[0026] Coptis chinensis extract has significant antibacterial effects and can inhibit the growth of a variety of oral pathogens. Coptis chinensis extract has a strong inhibitory effect on periodontal bacteria such as Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans. In addition, Coptis chinensis extract also shows certain antibacterial activity against Staphylococcus aureus. Coptis chinensis extract also has anti-inflammatory properties and can reduce oral mucosal inflammation. This anti-inflammatory effect is related to the alkaloids and flavonoids it contains, which can inhibit the activity of inflammation-related enzymes, thereby reducing inflammatory responses.

[0027] Furthermore, the nanocomposite material is Ag / TiO2 nanomaterial, and the particle size of the Ag / TiO2 nanomaterial is 55-200nm.

[0028] Silver nanoparticles are widely used in mouthwashes due to their significant antibacterial activity. Mouthwash containing silver nanoparticles can effectively inhibit the growth of bacteria in the mouth, thereby preventing the formation of dental plaque. In addition, the inhibitory effect of silver nanoparticle mouthwash on certain bacteria is even better than that of traditional mouthwash ingredients. Titanium dioxide nanoparticles have excellent photocatalytic properties and can produce strong oxidants under ultraviolet irradiation, destroying the cell membrane and protein structure of bacteria, thereby playing a bactericidal role. This property makes Ag / TiO2 nanomaterials have potential application value in oral care products, especially when enhanced antibacterial effects are needed.

[0029] Furthermore, the synergist is one or more of riboflavin, sodium chloride, peppermint essential oil, eucalyptus essential oil, ginger essential oil, methyl salicylate and lemon essential oil.

[0030] By utilizing the better penetration effect of plant essential oils, the secretion of oral saliva is stimulated, the formation of plaque biofilm is inhibited, the effect of cleaning the oral cavity and refreshing the breath is achieved, and through key factor analysis, an essential oil mouthwash with better comfort is obtained.

[0031] Furthermore, the preparation method of Ag / TiO2 nanomaterials includes the following steps: adding silver nitrate solution to TiO2 ethanol mixed solution, adding polyvinyl pyrrolidone ethanol solution, stirring at 70-80°C, centrifugally washing, and drying to obtain Ag / TiO2 nanomaterials.

[0032] Furthermore, the surfactant is one or more of PEG-40 hydrogenated castor oil, poloxamer, PEG-60 hydrogenated castor oil, polyethylene glycol, polypropylene glycol, polyoxyethylene oleate, and polyoxyethylene octylphenol ether.

[0033] Furthermore, the moisturizing agent is one or more of glycerol, xylitol, propylene glycol, 1,3-butylene glycol, polyethylene glycol, and sorbitol.

[0034] Furthermore, the preservative is one of methylparaben, sodium benzoate, methylparaben, and methylparaben.

[0035] Furthermore, the pH adjuster is benzoic acid or citric acid.

[0036] The method for preparing the above-mentioned color-changing antibacterial mouthwash comprises the following steps: (1) According to the formula, take surfactant, moisturizer, antibacterial agent, and synergist, heat to 60-90°C, stir and mix, add part of deionized water, keep at 70-90°C, stir for 1-1.5 hours, cool to room temperature, filter until the filtrate is clear, and prepare mixed solution A; (2) adding a preservative and a pH adjuster to the mixed solution A, stirring and mixing, and obtaining a mixed solution B; (3) Add the detection agent to the mixed solution B, stir and mix, and prepare a color-changing antibacterial mouthwash.

[0037] An application of a color-changing antibacterial mouthwash, wherein the color-changing antibacterial mouthwash is used to detect the pH value of the oral cavity. The specific detection method is: determining the pH value in the oral cavity based on the comparison between the final color of the mouthwash after use and the color of the mouthwash at different pH values.

[0038] Compared with the prior art, it has the following beneficial effects: 1. The above technical solution of the present invention monitors the pH of the oral cavity every time a mouthwash is used, so that the user can grasp the changes in the pH of the oral cavity in real time, and thus take corresponding measures in time to adjust the oral environment and maintain oral health.

[0039] 2. The various natural plant extracts (such as dandelion extract, schisandra chinensis bark extract, gentian extract, etc.) in the mouthwash prepared by the present invention have antibacterial, anti-inflammatory, heat-clearing and detoxifying effects, and can effectively inhibit the growth of bacteria in the oral cavity and reduce the occurrence of oral diseases.

[0040] 3. The present invention provides mouthwashes with different formulas to address the problems caused by abnormal oral pH (acidic or alkaline), which can alleviate problems such as gingivitis, bleeding gums, tooth decay, bad breath, etc. caused by oral pH imbalance, as well as permanent damage such as enamel dissolution caused by excessive oral acidity. The ingredients in the mouthwash are mainly natural plant extracts and common oral care ingredients, without chemical additives, safe to use, and suitable for long-term use. In addition to detecting oral pH and antibacterial, mouthwash also has multiple functions such as cleaning the mouth, covering up bad breath, and leaving the mouth with a comfortable and refreshing feeling. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] In the accompanying drawings, Figure 1 The corresponding relationship between the color of the antibacterial mouthwash after rinsing and the pH value of the oral cavity is as follows: 0—normal color (pH 6.6-7.1), 1—very unhealthy alkaline color (pH ≥ 8.2), 2—unhealthy alkaline color (pH 7.7-8.1), 3—relatively unhealthy alkaline color (pH 7.2-7.6), 4—very unhealthy acidic color (pH ≤ 5.5), 5—unhealthy acidic color (pH 5.6-6.0), 6—relatively unhealthy acidic color (pH 6.1-6.5). DETAILED DESCRIPTION

[0042] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the accompanying drawings and corresponding embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0043] Oral pH is also expressed as pH. Under normal circumstances, the pH value of the mouth should be between 6.6 and 7.1. This range of values ​​is ideal for maintaining oral health. The lower the pH value, the more acidic it is, and the higher the pH value, the more alkaline it is. Changes in oral pH will have different effects on oral health. When the oral pH value is low, that is, too acidic, it is easy to cause gingivitis, bleeding gums, tooth decay, bad breath and other problems. Excessive oral acidity may cause the dissolution of tooth enamel, which is a permanent damage to the teeth, causing the teeth to become brittle and easy to break.

[0044] The change of oral pH is affected by many factors. Generally speaking, bacteria in the mouth are the main factor in the change of acidity. These bacteria will use food residues to produce acidic substances, causing the pH value of the mouth to decrease. In addition, the acidity and alkalinity of food and beverages will also affect the pH value of the mouth. More acidic foods such as yogurt and lemon juice will increase the acidity of the mouth, while more alkaline foods such as fruits and vegetables are beneficial to oral health and can reduce oral acidity.

[0045] To control the changes in oral pH, you need to maintain good oral hygiene, including brushing, rinsing, and flossing. Reduce or avoid eating sweets, carbonated drinks, and other foods that easily produce acidic substances. Increase the proportion of alkaline foods such as fruits and vegetables in your diet, and go to the dentist for regular checkups and tooth cleanings to prevent the occurrence of oral diseases.

[0046] Therefore, the present invention innovatively combines color change detection technology with the antibacterial and anti-inflammatory effects of natural plant extracts to develop a mouthwash product that can not only detect oral pH but also effectively treat and prevent abnormal oral pH, providing users with a more comprehensive and convenient oral care solution.

[0047] In the present invention, multiple functions can be achieved by adding multiple pure plant extracts. According to the data and experimental data made by the inventor, for example, when the content of dandelion extract increases from 1% to 30%, its detumescence and detoxification effects are significantly improved, and it has certain relief and conditioning effects on patients with pharyngitis, acute tonsillitis, mumps, gastroenteritis, cholecystitis and other diseases. When the content of Houpu extract increases from 1% to 20%, it has significant functions of drying dampness and eliminating phlegm, lowering qi and eliminating accumulation, and improves the lungs and digestive tract, especially the digestive tract. The improvement effect will also be greatly improved with the increase of concentration. When the content of Atractylodes macrocephala extract reaches 0.1%, its functions of enhancing the body's immune function, anti-oxidation, and antibacterial are obviously reflected. When the content is further increased to 30%, its effect will increase accordingly and reach a limit value. When the content of licorice extract reaches 0.1%, it has obvious heat-clearing and detoxification effects. When the content exceeds 30%, the increase in the efficacy of licorice extract and the adverse effects of licorice extract on the product will be much more obvious. However, the plant extract ingredients will have an adverse effect on the texture of mouthwash. Therefore, the present invention can also neutralize the adverse effect of the extract ingredients of high embankment on the texture of mouthwash by adding glycerol as a moisturizing ingredient. At the same time, the sodium benzoate added by the present invention not only has the effect of antibacterial, antiseptic and moisturizing oral mucosa, but also forms a buffer system with benzoic acid. In the construction of the buffer system, benzoic acid and sodium benzoate play a core role and are the key elements to maintain the acid-base balance of the solution. As an important member in the chemical field, they can effectively deal with the pH fluctuations caused by external factors and ensure the acid-base stability of the solution. As an organic acid, benzoic acid can generate sodium benzoate with good water solubility after reacting with alkali, and this salt has laid a solid foundation for the preparation of buffer solution. The present invention is just by adding sodium benzoate and forming a buffer system with benzoic acid, protecting the activity of herbal plant extracts, and can avoid the pH value caused by the adjustment of ingredients. Changes greatly, thereby affecting the activity reduction caused by herbal plant extracts under acidic or alkaline conditions. The content of glycerol, menthol, and xylitol has played a better effect through scientific proportioning.

[0048] For example, glycerin is a common moisturizer, and its content in the product will significantly affect its moisturizing effect. Glycerin can moisturize the skin for a long time, and at the same time form a protective film on the surface of the skin to lock in moisture and reduce moisture loss from the skin. Therefore, when the glycerin content is higher, the moisturizing ability of the product will be stronger. However, glycerin may be sticky to use, so its content also needs to be appropriately adjusted according to the overall formula of the product and the consumer's experience. Menthol mainly plays a cooling and soothing role in the product. Its content will affect the cooling feeling and user experience of the product. The right amount of menthol can bring users a cool and comfortable feeling, especially in oral care products, where a moderate content of menthol can bring a fresh breath and a comfortable experience. However, if the menthol content is too high, it may cause excessive irritation to the user and affect the user experience of the product. The content of xylitol in the product is equally important. It can not only improve the taste of the product as a sweetener, but also has anti-caries and antibacterial effects. In oral care products, a proper amount of xylitol can effectively prevent caries and oral diseases, but if the content is too high, it will cause inappropriate and rapid growth of oral microorganisms.

[0049] Example 1 A method for preparing a color-changing antibacterial mouthwash comprises the following steps: Weigh 5g Ag / TiO2 nanomaterial, 10g glycerol, 2.1g sodium benzoate, and 1g benzoic acid respectively, put into 20g purified water, stir thoroughly to dissolve, keep warm at 70°C, stir for 1h, cool to room temperature, then add 10g hydrolyzed proanthocyanidins, 5g sodium chloride, 1.8g PEG-40 hydrogenated castor oil, 0.5g citric acid, 0.5g menthol, 2g xylitol, 5g riboflavin, and 1.5g edible flavor respectively, stir thoroughly, then weigh 2g dandelion extract, 2g thick pummelo bark extract, 3g gentian extract, 3g Atractylodes macrocephala extract, 1g licorice extract, 1g Codonopsis pilosula extract, and 5g Coptis chinensis extract, stir thoroughly, and after passing the test, package to obtain a color-changing antibacterial mouthwash that can alleviate alkaline oral problems.

[0050] The color-changing antibacterial mouthwash produced by the scheme described in this embodiment not only has pure natural herbal properties, but also can alleviate many troubles caused by the alkaline problem of the oral cavity, and is suitable for people with alkaline oral pH.

[0051] Example 2 A method for preparing a color-changing antibacterial mouthwash comprises the following steps: Weigh 8g Ag / TiO2 nanomaterial, 10g glycerol, 2.4g sodium benzoate, and 1.2g benzoic acid respectively, put into 25g purified water, stir thoroughly to dissolve, keep warm at 70°C, stir for 1.5h, cool to room temperature, add 10g hydrolyzed proanthocyanidins, 5g sodium chloride, 1.5g PEG-40 hydrogenated castor oil, 0.5g citric acid, 0.5g menthol, 5g xylitol, 5g riboflavin, and 1.5g edible flavor respectively, stir thoroughly, weigh 3g dandelion extract, 2g thick pummelo bark extract, 1g gentian extract, 1g Atractylodes macrocephala extract, 2g licorice extract, 2g Codonopsis pilosula extract, and 5g Coptis chinensis extract, stir thoroughly, and after passing the test, package to obtain a color-changing antibacterial mouthwash that can alleviate acidic oral problems.

[0052] The color-changing antibacterial mouthwash produced by the scheme described in this embodiment not only has pure natural herbal properties, but also can alleviate many problems caused by the acidic oral cavity problem, and is suitable for people with acidic oral pH.

[0053] Example 3 A method for preparing a color-changing antibacterial mouthwash comprises the following steps: Weigh 10g Ag / TiO2 nanomaterial, 10g glycerol, 1.8g sodium benzoate, and 1g benzoic acid respectively, put them into 28g purified water, stir them thoroughly to dissolve, keep them at 80℃, stir them for 1h, cool them to room temperature, then add 10g hydrolyzed proanthocyanidins, 5g sodium chloride, 2.5g PEG-40 hydrogenated castor oil, 0.5g citric acid, 0.5g menthol, 2.2g xylitol, 5g riboflavin, and 1.2g edible flavor respectively, stir them thoroughly, then weigh 2g dandelion extract, 3g thick pummelo bark extract, 1g gentian extract, 2g atractylodes extract, 2g licorice extract, 5g codonopsis extract, and 3g coptis extract, stir them thoroughly, and after passing the test, package them to obtain a color-changing antibacterial mouthwash that can more sensitively indicate the pH value of the oral cavity.

[0054] The color-changing antibacterial mouthwash produced by the scheme described in this embodiment not only has pure natural herbal properties, but is also more sensitive in detecting oral pH and is more suitable for use in detecting oral pH during regular mouthwash.

[0055] Example 4 A method for preparing a color-changing antibacterial mouthwash comprises the following steps: Weigh 4g Ag / TiO2 nanomaterial, 10g glycerol, 2.1g sodium benzoate, and 1g benzoic acid respectively, put into 30g purified water, stir thoroughly to dissolve, keep warm at 80°C, stir for 1.5h, cool to room temperature, add 2g hydrolyzed proanthocyanidins, 1g sodium chloride, 1.5g PEG-40 hydrogenated castor oil, 0.5g citric acid, 0.5g menthol, 1.6g xylitol, 5g riboflavin, and 1.5g edible flavor respectively, stir thoroughly, weigh 3g dandelion extract, 2g thick pummelo bark extract, 3g gentian extract, 5g Atractylodes macrocephala extract, 2g licorice extract, 2g Codonopsis pilosula extract, and 1g Coptis chinensis extract, stir thoroughly, and package after passing the test to obtain a color-changing antibacterial mouthwash that can relieve oral ulcers.

[0056] The color-changing antibacterial mouthwash produced by the scheme described in this embodiment not only has pure natural herbal properties, but also can relieve some inflammatory oral problems, such as oral ulcers, and is suitable for people who often suffer from oral ulcers.

[0057] According to the above-mentioned embodiments 1-4 of the present invention, natural plant extracts such as dandelion, thick pu bark, gentian, atractylodes, licorice, codonopsis, and coptis are used to exert their antibacterial, anti-inflammatory, heat-clearing and detoxifying effects, effectively inhibit the growth of oral bacteria, and maintain oral health. The combined use of natural plant extracts such as dandelion, thick pu bark, and gentian in mouthwash can exert their respective antibacterial, anti-inflammatory, heat-clearing and detoxifying effects, and there is also a synergistic effect between these ingredients, which can enhance the overall antibacterial effect of the mouthwash and improve the efficiency of oral care.

[0058] Magnolia bark extract can dry out dampness and eliminate phlegm, relieve gas and eliminate accumulation, and adjust the lungs and digestive tract; tea polyphenols in tea extract can prevent caries and strengthen teeth; citric acid has the effect of freshening breath and cleaning tartar. The synergistic effect of these ingredients makes the mouthwash provide comprehensive oral health protection while being antibacterial. By precisely controlling the proportion and dosage of each ingredient, the formula of the mouthwash is optimized, so that it has both color change detection function and good oral care effect, and the mouthwash has stable performance and significant effect. By adding color-changing ingredients such as riboflavin, the mouthwash can change color according to the pH change of the oral cavity after contact with the oral cavity, thereby realizing real-time monitoring of the pH of the oral cavity and self-detection of the pH of the oral cavity. The combination of natural plant extracts with oral care ingredients such as nanomaterials, glycerin, xylitol, sodium benzoate, etc., not only enhances the antibacterial effect of the mouthwash, but also improves its ability to clean the oral cavity, cover up bad breath, and leave a comfortable and refreshing feeling in the oral cavity. This synergistic effect enables the mouthwash to play an excellent role in many aspects and meet the diverse needs of users for oral care.

[0059] Application Example 1 This application example is the application of the color-changing antibacterial mouthwash prepared in Example 1.

[0060] Pour about 15 ml of the color-changing antibacterial mouthwash prepared in Example 1 of the present invention into a transparent or white container, gargle in the mouth for 40 to 60 seconds to allow the mouthwash to fully contact the mouth, and then spit it out into the container, observe the final color of the mouthwash after use, and determine the pH in the mouth by comparing the final color of the mouthwash with the final color of the mouthwash under different pH values, as shown in the attached figure. Figure 1 shown.

[0061] Application Example 2 This application example is the application of the color-changing antibacterial mouthwash prepared in Example 2.

[0062] Pour about 15 ml of the color-changing antibacterial mouthwash prepared in Example 2 of the present invention into a transparent or white container, gargle in the mouth for 40 to 60 seconds to allow the mouthwash to fully contact the mouth, then spit it out into the container, observe the final color of the mouthwash after use, and determine the pH in the mouth based on the comparison between the final color of the mouthwash and the final color of the mouthwash at different pH values.

[0063] Application Example 3 This application example is the application of the color-changing antibacterial mouthwash prepared in Example 3.

[0064] Pour about 15 ml of the color-changing antibacterial mouthwash prepared in Example 3 of the present invention into a transparent or white container, gargle in the mouth for 40 to 60 seconds to allow the mouthwash to fully contact the mouth, then spit it out into the container, observe the final color of the mouthwash after use, and determine the pH in the mouth based on the comparison between the final color of the mouthwash and the final color of the mouthwash at different pH values.

[0065] Application Example 4 This application example is the application of the color-changing antibacterial mouthwash prepared in Example 3.

[0066] Pour about 15 ml of the color-changing antibacterial mouthwash prepared in Example 3 of the present invention into a transparent or white container, gargle in the mouth for 40 to 60 seconds to allow the mouthwash to fully contact the mouth, then spit it out into the container, observe the final color of the mouthwash after use, and determine the pH in the mouth based on the comparison between the final color of the mouthwash and the final color of the mouthwash at different pH values.

[0067] If the oral pH is normal, it will show a normal color 0, and the oral pH is: pH6.6-7.1. If the oral pH is alkaline, depending on the severity of the abnormality, when pH≥8.2, it will show a very unhealthy alkaline color 1, when pH7.7-8.1, it will show an unhealthy alkaline color 2, and when pH7.2-7.6, it will show a relatively unhealthy alkaline color 3; if the oral pH is acidic, depending on the severity of the abnormality, when pH≤5.5, it will show a very unhealthy acidic color 4, when pH5.6-6.0, it will show an unhealthy acidic color 5, and when pH6.1-6.5, it will show a relatively unhealthy acidic color 6.

[0068] Comparative experiment: Select New Zealand rabbits, ordinary grade, weight: 2.0Kg ~ 3.0Kg, a total of 6 males as test animals. Provided by Jiashan County Golden Rabbit Rabbit Industry Professional Cooperative, production license number: SCXK (Zhe) 2020-0006, quality certificate number: 20221201Ceez0600000951.

[0069] Animal feeding and management conditions: temperature 20℃~26℃, relative humidity 40%~70%, light 12h alternating light and dark. Free drinking water, feed with standard block maintenance feed, animal feed purchased from Jiangsu Collaborative Pharmaceutical Bioengineering Co., Ltd. (production license number: Su Feed Certificate (2019) 01008), feed batch number: 22100221.

[0070] Establishing oral ulcer animal model by chemical cauterization: Take an adult healthy rabbit, fix it vertically on the surface of the rabbit's oral mucosa with a glass tube with an inner diameter of 0.6 cm and a length of 3 cm, inject 0.2 mL of glacial acetic acid with a volume fraction of 50% into the tube, and dip out the glacial acetic acid with a cotton swab after 30 seconds. After 24 hours, the area of ​​mucosal ulcers was measured and the inflammation and congestion were observed to confirm that the modeling was successful. After the modeling was successful, the experimental groups were divided into the following groups: the blank group was numbered A and the ulcer was not treated, the control group was numbered B and the ulcer was treated with purified water, and the ulcers in the experimental group were treated with samples produced according to Examples 1 to 4 of the present invention, respectively, and the rabbits treated with the samples produced in Example 1 were numbered C, and the rabbits treated with the samples produced in 2 were numbered D, and the rabbits treated with the samples produced in Example 3 were numbered E, and the rabbits treated with the samples produced in Example 4 were numbered F.

[0071] The model was successfully established on day 0. From day 0, feed 260g of feed every morning. The remaining amount was weighed on the second day to calculate the food intake. Use a medical cotton swab to dip the sample to be tested or purified water to wipe the affected area of ​​the rabbit's oral ulcer. Do it once before eating in the morning, noon, and evening, and eat 30 minutes later. Observe the rabbits every day, record: weight, eating conditions, take photos of the ulcer surface, and make a comprehensive score. The specific scores are shown in Table 1 below: Table 1 Comprehensive score (scored according to the reduction in ulcer area) In the table, 5 points represent complete recovery. From the experimental results, it can be seen that the ulcer surface of the blank group did not recover naturally during the entire experimental period, indicating that oral ulcers are difficult to heal naturally. The ulcer surface of the control group also did not recover significantly, and the score was similar to that of the blank group, indicating that purified water has little effect on the treatment of oral ulcers.

[0072] Except for the blank group and the control group, the ulcer surfaces of the remaining rabbits recovered within the thirteenth day. The ulcer surfaces of the rabbits treated with the samples produced in Example 4 recovered the fastest. From the second day, it was obvious that the ulcer surface became smaller, and it was completely recovered on the ninth day, indicating that the mouthwash produced in Example 4 has significant advantages in the treatment of oral ulcers. The ulcer surfaces of the rabbits treated with the samples produced in Example 1, Example 2, and Example 3 recovered slightly slower, starting to recover from the fifth day, the fifth day, and the fourth day, respectively, and fully recovered on the thirteenth day, the thirteenth day, and the twelfth day, respectively.

[0073] Through comparative experiments, it can be seen that the color-changing antibacterial mouthwashes produced in Examples 1 to 4 of the present invention all have certain therapeutic effects on oral ulcers. Among them, the mouthwash produced in Example 4 has the most outstanding performance in treating oral ulcers, with the fastest recovery speed and the best therapeutic effect. This may be related to the proportion and formula of the specific ingredients in Example 4, which makes it have stronger efficacy in antibacterial, anti-inflammatory, and promoting the healing of oral ulcers.

[0074] Performance Testing: This example is a quantitative antibacterial test of the color-changing antibacterial mouthwash prepared in Examples 1 to 4 on a suspension of Helicobacter pylori.

[0075] Take a 24-hour fresh slant culture of Helicobacter pylori and wash it with PBS, dilute it with PBS to about 5.0X10 5 CFU / mL~4.5x10 6CFU / mL bacterial suspension is ready for use. Take four sterile test tubes, numbered 1 to 4, and add 5.0mL sample stock solution of the color-changing antibacterial mouthwash prepared in Examples 1 to 4 respectively. After placing in a 20℃±1℃ water bath for 5 minutes, add 0.1mL of the test bacterial suspension, mix quickly and start timing immediately. After the test bacteria interact with the sample for 10 minutes, take 1.0mL of the test bacteria and sample mixture to inoculate 2 plates respectively, and pour the culture medium. When the amount of bacteria cannot be counted, make a 10-fold serial dilution with PBS, select the appropriate dilution and take 1.0mL to inoculate 2 plates respectively, and count the live bacteria culture. At the same time, use PBS instead of the sample to conduct a parallel test as a positive control. The number of colonies recovered in the positive control is 1.0x10 4 CFU / mL~9.0x10 4 CFU / mL. Take the same batch of PBS and culture medium as negative control. All test samples and control samples are cultured at 36℃±1℃, and the bacterial propagules are cultured for 48h to observe the final results. The test is repeated 3 times, and the inhibition rate is calculated according to the following formula: ×100% Where: X—inhibition rate, %; —The amount of bacteria recovered in the positive control group, in CFU / mL; —The amount of bacteria recovered in the test group, in CFU / mL.

[0076] When the antibacterial rate is ≥50% to 90%, it is judged to have antibacterial effect; when the antibacterial rate is ≥90%, it is judged to have strong antibacterial effect. The test results are shown in the following table: Table 2 Suspension quantitative antibacterial test data (recovered bacteria, unit is CFU / mL) It can be seen from the above table that the antibacterial rate of the color-changing antibacterial mouthwashes prepared in Examples 1 to 4 of the present invention against Helicobacter pylori reaches 99.99%.

[0077] In summary, the color-changing antibacterial mouthwash of the present invention not only has the function of color-changing detection of oral pH, but also can effectively treat oral problems such as oral ulcers, and has broad market application prospects and important practical significance.

[0078] It should be understood that the various forms of processes shown above can be used to reorder, add or delete steps. For example, the steps described in the present invention can be executed in parallel, sequentially or in different orders, as long as the desired results of the technical solution disclosed in the present invention can be achieved, and this document does not limit this.

[0079] The above specific implementations do not constitute a limitation on the protection scope of the present invention. It should be understood by those skilled in the art that various modifications, combinations, sub-combinations and substitutions can be made according to design requirements and other factors. Any modification, equivalent substitution and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A color-changing antibacterial mouthwash, characterized in that: The invention is composed of the following raw materials in percentage by mass: 1-30% antibacterial agent, 1-20% synergist, 0.5-5% surfactant, 20-30% moisturizer, 0.5-3% preservative, 0.2-1.2% pH regulator and 1-20% detection agent; the antibacterial agent comprises a composite extract and a nano-composite material, the composite extract is a dandelion extract, a thick mulberry bark extract, a gentian extract, a white atractylodes extract, a licorice extract, a codonopsis extract and a coptis extract; the detection agent is a hydrolyzed proanthocyanidin.

2. A color-changing antibacterial mouthwash according to claim 1, characterized in that: The nano-composite material is Ag / TiO2 nano-material, and the particle size of the Ag / TiO2 nano-material is 55-200nm.

3. A color-changing antibacterial mouthwash according to claim 1, characterized in that: The synergistic enhancer is one or more of riboflavin, sodium chloride, peppermint essential oil, eucalyptus essential oil, ginger essential oil, methyl salicylate and lemon essential oil.

4. A color-changing antibacterial mouthwash according to claim 2, characterized in that: The preparation method of Ag / TiO2 nano material comprises the following steps: adding silver nitrate solution into TiO2 ethanol mixed solution, adding polyvinyl pyrrolidone ethanol solution, stirring at 70-80 DEG C, centrifugally washing, and drying to obtain Ag / TiO2 nano material.

5. A color-changing antibacterial mouthwash according to claim 1, characterized in that: The surfactant is one or more of PEG-40 hydrogenated castor oil, poloxamer, PEG-60 hydrogenated castor oil, polyethylene glycol, polypropylene glycol, polyoxyethylene oleate, and octylphenol polyoxyethylene ether.

6. A color-changing antibacterial mouthwash according to claim 1, characterized in that: The moisturizer is one or more of glycerin, xylitol, propylene glycol, 1,3-butylene glycol, polyethylene glycol, and sorbitol.

7. A color-changing antibacterial mouthwash according to claim 1, characterized in that: The preservative is one of methylparaben, sodium benzoate, methylparaben, and methylparaben.

8. A color-changing antibacterial mouthwash according to claim 1, characterized in that: The pH adjusters are benzoic acid and citric acid.

9. The method for preparing the color-changing antibacterial mouthwash according to any one of claims 1 to 8, characterized in that: The following steps are involved: (1) According to the formula, take surfactant, moisturizer, antibacterial agent, and synergist, heat to 60-90°C, stir and mix, add part of deionized water, keep at 70-90°C, stir for 1-1.5 hours, cool to room temperature, filter until the filtrate is clear, and prepare mixed solution A; (2) adding a preservative and a pH adjuster to the mixed solution A, stirring and mixing, and obtaining a mixed solution B; (3) Add the detection agent to the mixed solution B, stir and mix, and prepare a color-changing antibacterial mouthwash.

10. The use of a color-changing antibacterial mouthwash according to claim 1, characterized in that: The color-changing antibacterial mouthwash is used to detect the pH value of the oral cavity. The specific detection method is: the pH value in the oral cavity is determined by comparing the final color of the mouthwash after use with the color of the mouthwash under different pH values.

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