Borsella strain, fungicide, application and method

Abstract

The invention discloses a Borsella strain, a fungicide, application and a method. The Borsella strain provided by the invention is a Borsella QY2 (Bosea sp.QY2) strain, and the preservation number of the Borsella strain is CCTCC (China Center for Type Culture Collection) NO: M 20242096. The Borsella QY2 strain disclosed by the invention can grow by taking polyethylene as a unique carbon source, and can tolerate polyethylene plastic with the concentration of 1-1000mg / L. The strain can significantly reduce infrared spectrum characteristic functional groups of polyethylene plastics and characteristic products identified by pyrolysis chromatography-mass spectrometry, and can change the thermodynamic properties of polyethylene, which shows that the Borsella QY2 strain can utilize polyethylene plastics. The discovery of the strain provides a new thought and method for degrading polyethylene plastics in various environments, and is beneficial to the treatment of environmental pollution and the health of human beings.

Description

Technical Field

[0001] The present invention generally relates to microbial technology, and in particular to a Bosnerella QY2 strain, a bacterial agent containing the same, a use thereof, and a method for degrading polyethylene. Background Art

[0002] With the development of the chemical industry, plastic products are widely used in various packaging materials (polyethylene PE), beverage bottles (polyester plastics, PET) and foam pads and fast food lunch boxes (polystyrene plastics PS) due to their lightness, durability, cheapness and convenience. Plastic pollution is one of the most pressing ecological and health issues in the world today. Since most plastics are composed of stable long-chain polymers, they are not easily degraded in the environment, and the degradation process is extremely slow.

[0003] The chemical structure of polyethylene consists of repeating -[CH2-CH2] n -unit composition, this material is stable due to its large molecular weight and strong hydrophobicity. In particular, its strong CC chain skeleton and the lack of enzymes in nature to decompose these chains make it difficult to biodegrade under natural conditions, and thus easily accumulate in the environment. At present, the methods for treating waste plastics mainly include landfill, burning and reprocessing, which not only cause serious pollution to the environment, but also have high treatment costs and may cause secondary pollution problems. In view of this, it has become particularly important to explore safe and green treatment technologies for polyethylene plastic waste. At present, there are relatively few studies on the biological treatment of polyethylene plastic waste, the resources of efficient strains are limited, and the treatment efficiency is low. The development of strains that can degrade polyethylene plastics is an urgent problem to be solved. Summary of the invention

[0004] Based on this, the present application provides a Bosea sp. strain capable of degrading polyethylene, wherein the Bosea sp. strain is Bosea sp. QY2 strain, and the deposit number is CCTCC NO: M20242096.

[0005] In another aspect, the present application also provides a bacterial agent comprising the Bosteria QY2 strain described herein.

[0006] In another aspect, the present application also provides use of the Bosnerella QY2 strain described herein or the bacterial agent described herein in degrading polyethylene.

[0007] In another aspect, the present application also provides a method for degrading polyethylene, wherein the Bosnerella QY2 strain described herein or the bacterial agent described herein is co-cultured with polyethylene.

[0008] The present application provides a strain of Bosea sp., which is named as Bosea sp. QY2 strain. It is deposited in China Center for Type Culture Collection, address: No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, and the deposit date is September 26, 2024. The deposit number is CCTCC NO: M 20242096.

[0009] The Bosnerella QY2 strain of the present application can grow with polyethylene as the sole carbon source and can tolerate polyethylene plastics at a concentration of 1-1000 mg / L. The strain can significantly reduce the characteristic functional groups of the infrared spectrum of polyethylene plastics and the characteristic products identified by pyrolysis chromatography mass spectrometry, and the strain can change the thermodynamic properties of polyethylene, indicating that the Bosnerella QY2 strain can utilize polyethylene plastics. The discovery of the strain provides new ideas and methods for degrading polyethylene plastics in various environments, which is beneficial to the control of environmental pollution and human health.

[0010] Other features and advantages of the present application will be described in the following description, and partly become apparent from the description, or be understood by implementing the present application. Other advantages of the present application can be realized and obtained by the schemes described in the description and the drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] The accompanying drawings are used to provide an understanding of the technical solution of the present application and constitute a part of the specification. Together with the embodiments of the present application, they are used to explain the technical solution of the present application and do not constitute a limitation on the technical solution of the present application.

[0012] Figure 1 The growth of the Bosnerella QY2 strain isolated and purified in the present application in a solid culture medium with or without the addition of high-density polyethylene (HDPE) aging solution is shown. The two pictures in the first row are the front side of the culture dish, and the two pictures in the second row are the back side of the culture dish.

[0013] Figure 2 The colony status of the Bosella QY2 strain of the present application at different growth times in a solid culture medium with or without the addition of HDPE powder is shown.

[0014] Figure 3 This is an infrared spectrum of the treatment with Bosella QY2 strain added and HDPE control in Example 2 of the present application.

[0015] Figure 4 This is the pyrolysis chromatogram mass spectrum of the treatment with Bosella QY2 strain added and HDPE control in Example 2 of the present application.

[0016] Figure 5This is a differential scanning calorimetry (DSC) spectrum of the treatment with Bosella QY2 strain added and HDPE control in Example 2 of the present application. DETAILED DESCRIPTION

[0017] Unless otherwise stated, the technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art to which the application belongs. When a certain amount, concentration or other value or parameter is expressed in the form of a range, a preferred range, or a preferred upper numerical limit and a preferred lower numerical limit, it should be understood that it is equivalent to specifically revealing any range by combining any pair of upper range limits or preferred numerical values ​​with any lower range limit or preferred numerical value, regardless of whether the range is specifically disclosed. Unless otherwise stated, the numerical ranges listed herein are intended to include the endpoints of the range and all integers and fractions (decimals) within the range.

[0018] The terms "about" and "approximately" when used with a numerical variable generally refer to the value of that variable and all values ​​of that variable are within experimental error (e.g., within a 95% confidence interval of the mean) or within ±10% of the specified value, or a wider range.

[0019] The expression "comprising" or its synonymous similar expressions "including", "containing" and "having" etc. are open-ended and do not exclude additional unrecited elements, steps or ingredients. The expression "consisting of excludes any element, step or ingredient not specified. The expression "consisting essentially of means that the scope is limited to the specified elements, steps or ingredients, plus the optional elements, steps or ingredients that do not materially affect the basic and novel characteristics of the claimed subject matter. It should be understood that the expression "comprising" encompasses the expressions "consisting essentially of" and "consisting of".

[0020] The expression "at least one" or "one or more" means 1, 2, 3, 4, 5, 6, 7, 8, 9 or more.

[0021] The present application provides a Bosea sp. strain, wherein the Bosea sp. strain is a Bosea sp. QY2 strain, and the deposit number is CCTCC NO: M 20242096.

[0022] In another aspect, the present application also provides a bacterial agent comprising the Bosteria QY2 strain described herein.

[0023] In some embodiments, the bacterial agents described herein comprise live bacteria of the Bosnerella strain QY2.

[0024] In some embodiments, the bacterial agents described herein contain the Bosnerella QY2 strain as the sole active ingredient.

[0025] In some embodiments, the bacterial agent described herein further comprises other active ingredients. In some embodiments, the bacterial agent described herein further comprises one or more other active ingredients that can act synergistically with the Bosnerella QY2 strain. In some embodiments, the other active ingredients are one or more other compounds or strains that can act synergistically with the Bosnerella QY2 strain. In some embodiments, the other active ingredients are one or more compounds that can react with plastics (e.g., polyethylene plastics, polystyrene plastics, or polyester plastics). In some embodiments, the other active ingredients are one or more other strains that can degrade plastics (e.g., polyethylene plastics, polystyrene plastics, or polyester plastics). Synergistic effects include promoting the growth and reproduction of the Bosnerella QY2 strain, enhancing the plastic degradation ability of the Bosnerella QY2 strain, and having a wider range of uses or functions after compounding with the Bosnerella QY2 strain.

[0026] In some embodiments, the bacterial agent described herein further comprises other substances that provide conditions for the degradation of plastic by the Bosnerella QY2 strain. In some embodiments, the bacterial agent described herein further comprises a culture medium that provides nutrients other than a carbon source for the growth of the Bosnerella QY2 strain. In some embodiments, the bacterial agent described herein further comprises a culture medium that promotes the growth of the Bosnerella QY2 strain. In some embodiments, the bacterial agent described herein further comprises a PPM culture medium.

[0027] In some embodiments, the formula of the solid plastic degrading bacteria purification medium (Plastic Degrading Bacteria Purification Medium, PPM medium) with plastic aging liquid added as the sole carbon source is 1g KH2PO4, 3g K2HPO4·3H2O, 0.2g MgSO4, 1g NaCl, 0.01g CaCl2, 0.006g Na2SeO35H2O, 0.008g Na2WO42H2O, 0.5g EDTA, 0.2g 7H2O·Fe2SO4, 0.01g 7H2O·ZnSO4, 0.003g 4H2O·MnCl2, 0.03g H3BO3, 0.02g 6H2O·CoCl2, 0.001g 2H2O·CuCl2, 0.002g 6H2O·NiCl2, 0.003g 2H2O·NaMoO4, 15g agar and 1L ultrapure water, and the plastic aging liquid added after high temperature and high pressure sterilization and cooling.

[0028] In another aspect, the present application also provides use of the Bosnerella QY2 strain described herein or the bacterial agent described herein in degrading polyethylene.

[0029] In some embodiments, the Bosnerella QY2 strain described herein can degrade polyethylene alone in the environment. In some embodiments, the Bosnerella QY2 strain described herein can degrade polyethylene in the environment together with other microorganisms. In some embodiments, the Bosnerella QY2 strain described herein can degrade polyethylene together with other active ingredients in the bacterial agent described herein. In some embodiments, the Bosnerella QY2 strain described herein can be used to treat plastic pollutants in the environment together with one or more other strains capable of degrading plastics (e.g., polyethylene plastics, polystyrene plastics, or polyester plastics). In some embodiments, the Bosnerella QY2 strain described herein can be used to treat various pollutants in the environment together with one or more other strains capable of degrading other pollutants.

[0030] In some embodiments, bacteria that can cooperate with the Bosella QY2 strain of the present application include Pseudomonas sp., Chryseobacterium sp., Alternaria sp., and Bacillus sp., etc.

[0031] In some embodiments, the degradation is that the Bosnerella QY2 strain grows on polyethylene as the sole carbon source and gradually degrades polyethylene.

[0032] In some embodiments, the polyethylene is selected from one or more of low density polyethylene, medium density polyethylene, and high density polyethylene.

[0033] In some embodiments, the polyethylene is high density polyethylene.

[0034] In some embodiments, the polyethylene is a granule, film or powder of polyethylene. In some embodiments, the polyethylene includes at least one of various particle sizes and specifications. In some embodiments, the plastic includes plastics of various types and sources. In some embodiments, the polyethylene plastic is aged plastic.

[0035] In some embodiments, the Bosnerella QY2 strain can degrade polyethylene in different water bodies (e.g., river water, lake water, ocean or sewage). In some embodiments, the Bosnerella QY2 strain can degrade plastics in the form of micro-nano particles in different water bodies (e.g., river water, lake water, ocean or sewage). In some embodiments, the Bosnerella QY2 strain can degrade polyethylene in the soil of a contaminated site. In some embodiments, the Bosnerella QY2 strain can degrade polyethylene in a landfill.

[0036] In some embodiments, the Bosurella QY2 strain can be used together with other strains to degrade sediments in different water bodies (e.g., river water, lake water, ocean or sewage). In some embodiments, the Bosurella QY2 strain can be used together with other strains to degrade plastics in the form of micro-nano particles in different water bodies (e.g., river water, lake water, ocean or sewage). In some embodiments, the Bosurella QY2 strain can be used together with other strains to degrade various garbage pollutants in landfills. In some embodiments, the Bosurella QY2 strain can produce a synergistic effect with other strains and be used together to treat sewage and / or contaminated soil. In some embodiments, the Bosurella QY2 strain can produce a synergistic effect with other strains and be used together for the degradation of garbage in landfills and the material energy cycle.

[0037] In another aspect, the present application also provides a method for degrading polyethylene, wherein the Bosnerella QY2 strain described herein or the bacterial agent described herein is co-cultured with polyethylene.

[0038] The present application describes multiple embodiments, but the description is exemplary rather than restrictive, and it is obvious to those skilled in the art that there may be more embodiments and implementations within the scope of the embodiments described in the present application. Although many possible feature combinations are shown in the drawings and discussed in the specific embodiments, many other combinations of the disclosed features are also possible. Unless specifically limited, any feature or element of any embodiment may be used in combination with any other feature or element in any other embodiment, or may replace any other feature or element in any other embodiment.

[0039] The present application includes and contemplates combinations of features and elements known to those of ordinary skill in the art. The embodiments, features and elements disclosed in the present application may also be combined with any conventional features or elements to form a unique invention scheme. Any features or elements of any embodiment may also be combined with features or elements from other invention schemes to form another unique invention scheme. Therefore, it should be understood that any feature shown and / or discussed in the present application may be implemented individually or in any appropriate combination. Therefore, except for the limitations made according to the attached claims and their equivalents, the embodiments are not subject to other restrictions. In addition, various modifications and changes may be made within the scope of protection of the attached claims.

[0040] In addition, when describing representative embodiments, the specification may have presented the method and / or process as a specific sequence of steps. However, to the extent that the method or process does not rely on the specific order of the steps described herein, the method or process should not be limited to the steps of the specific order described. As will be understood by those of ordinary skill in the art, other sequences of steps are also possible. Therefore, the specific sequence of the steps set forth in the specification should not be interpreted as a limitation to the claims. In addition, the claims for the method and / or process should not be limited to the steps of performing them in the order written, and those skilled in the art can easily understand that these sequences can be changed and still remain within the spirit and scope of the embodiments of the present application.

[0041] The experimental methods in the following examples without specifying specific conditions are usually measured according to national standards. The experimental materials in the following examples without specifying the source are all commercially available raw materials. The equipment used in each step in the following examples is conventional equipment. If there is no corresponding national standard, it is carried out according to the general international standards, conventional conditions, or according to the conditions recommended by the manufacturer. Unless otherwise defined or specified, all professional and scientific terms used in this application have the same meaning as those familiar to those skilled in the art. In addition, any method and material similar or equal to the recorded content can be applied to the method of this application.

[0042] Example

[0043] The relevant materials used in the examples are as follows:

[0044] 1. Reagents and Materials

[0045] (NH4)2SO4, KNO3, NaCl, C5H 14 ClNO, C 18 H 32 CaN2O 10 , C 19 H 19 N7O6, C6H6N2O, C8H9NO3, C 17 H 20 N4O6、C 12 H 17 ClN4OS·HCl and C6H 12O6, KH2PO4, K2HPO4·3H2O, MgSO4, NaCl, CaCl2, Na2SeO35H2O, Na2WO42H2O, EDTA, 7H2O·Fe2SO4, 7H2O·ZnSO4, 4H2O·MnCl2, H3BO3, 6H2O·CoCl2, 2H2O·CuCl2, 6H2O·NiCl2, 2H2O·NaMoO4 and other culture medium formula reagents are all analytically pure, and BG11 culture medium is purchased from Sinopharm Group, Qingdao Haibo Biotechnology, Maclean, Aladdin, Sigma, etc. Agar is purified agar. Microplastics are 80 mesh high-density polyethylene plastics (Sinopec).

[0046] 2. Instruments

[0047] Routine operation culture instruments include high temperature and high pressure sterilizer, clean bench, shaker, constant temperature incubator, etc. LED ultraviolet lamp with a wavelength of 395nm is used to irradiate the aged microplastics. Detection instruments include infrared spectrometer-microscope (Thermoelectric (Shanghai) Instrument Co., Ltd., Nicolet 6700FTIR), pyrolysis chromatography mass spectrometer (Shimadzu, GCMS-QP2010SE), differential scanning calorimeter (TA INSTRUMENTS, Q5000IR), etc.

[0048] Example 1. High-throughput screening method for plastic-degrading bacteria to isolate polyethylene-degrading bacteria

[0049] This application uses a high-throughput plastic-degrading bacteria enrichment method, which first adds mineral water to the soil of the contaminated site and oscillates for 24 hours to make a bacterial suspension; then adds it to a liquid plastic-degrading bacteria enrichment medium (BG11 medium) to activate the bacterial solution, adds a pretreated polyethylene solution and oscillates for 48 hours; finally, in a solid plastic-degrading bacteria purification medium (PPM medium) with polyethylene aging liquid as the only carbon source, further separates and purifies polyethylene-degrading bacteria. The relevant specific processing is as follows.

[0050] During polyethylene pretreatment, polyethylene micro-nano plastics were added to 100 mL of water to prepare a 10 g / L plastic mother liquor; under an LED lamp (395 nm wavelength) simulating natural ultraviolet radiation, the beaker was placed in a magnetic stirrer and stirred continuously to simulate environmental aging, and incubated at room temperature of 25°C for 7 days.

[0051] Commercially available BG11 culture medium powder was used to prepare liquid plastic-degrading bacteria enrichment culture medium. The formula of the purified culture medium of solid plastic-degrading bacteria (PPM culture medium) with plastic aging liquid added as the sole carbon source is: 1g KH2PO4, 3g K2HPO4·3H2O, 0.2g MgSO4, 1g NaCl, 0.01g CaCl2, 0.006g Na2SeO35H2O, 0.008g Na2WO42H2O, 0.5g EDTA, 0.2g 7H2O·Fe2SO4, 0.01g 7H2O·ZnSO4, 0.003g 4H2O·MnCl2, 0.03g H3BO3, 0.02g 6H2O·CoCl2, 0.001g 2H2O·CuCl2, 0.002g 6H2O·NiCl2, 0.003g 2H2O·NaMoO4, 15g agar, and 1L ultrapure water. When preparing BG11 and PPM culture media, mix the culture medium powder thoroughly and dissolve it in ultrapure water. After high temperature and high pressure sterilization, BG11 culture medium can be used directly after cooling. PPM culture medium needs to be cooled to about 60°C and mixed with plastic mother liquor, then poured into the microplate wells to cool and solidify for use.

[0052] Taking high-density polyethylene (HDPE) as an example, the process of separating plastic-degrading bacteria by the well plate plastic enrichment method of the present application is explained. First, in a conical flask, add 10g of soil from a plastic-contaminated site (isolated from a garbage dump in Beijing) and 100mL of Nongfu Spring Changbai Mountain mineral water, and culture at 30°C, 180rpm / min on a shaker for 24 hours to prepare a soil bacterial suspension; then add 100μL of soil bacterial suspension and 800μL of low-nutrient liquid plastic-degrading bacteria enrichment medium (BG11 medium) to each well of a 48-well plate and shake for activation for 24 hours; continue to add 100μL of aged 80-mesh polyethylene mother liquor (final polyethylene concentration is 1000mg / L), shake at 30°C, 80rpm / min on a shaker. After 48 hours of cultivation, the degradation bacteria were further domesticated. Finally, polyethylene mother liquor was added to the PPM medium to make the final concentration of polyethylene 1000 mg / L. No polyethylene was added as a carbon-free and nitrogen-free control. The degradation bacteria domesticated in the previous step were inoculated and streaked onto the solid PPM medium. Cultured at 30°C for 7-20 days, the growth of bacterial colonies was observed and photographed. Bacteria that can form colonies on PPM plates with polyethylene as the only carbon source but cannot grow in solid culture media without plastic are judged to have polyethylene degradation potential. Single colonies with plastic degradation potential are selected and isolated and purified multiple times to finally obtain pure polyethylene-degrading bacteria. The growth of the strain in the culture medium with or without the addition of HDPE aging liquid as the carbon source is shown in the figure. Figure 1The strain was identified as Bosea sp. by 16S rDNA sequencing (its 16S rDNA sequence is shown in SEQ ID NO: 1), and was named Bosea sp. QY2 strain.

[0053] It was deposited in China Center for Type Culture Collection, address: No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, and the deposit date is September 26, 2024. The deposit number is CCTCC NO: M 20242096.

[0054] Example 2. Growth of Bosella QY2 strain in plastic degradation medium

[0055] The growth of Bosnerella QY2 strain in 80 mesh high-density polyethylene was verified in a solid plastic degradation medium (Plastic Degrading Medium, PDM medium) with plastic powder as the only carbon source. Single colonies of Bosnerella QY2 strain were picked and streaked on plates with or without 1000 mg / L HDPE plastic added, and cultured in a 30°C constant temperature shaker for 2-10 days. The growth of the colonies was observed and photographed to determine the concentration range of polyethylene that can grow colonies. The growth results of Bosnerella QY2 strain are shown in Figure 2. Figure 2 As shown, the results show that the Bosnerella QY2 strain cannot grow in a carbon-free and nitrogen-free medium without polyethylene plastic, but grows well on 1000 mg / L polyethylene plastic and can form obvious colonies, indicating that the Bosnerella QY2 strain can grow with polyethylene as the only carbon source. In addition, Bosnerella QY2 cultured in PDM medium was used as a treatment, and only PDM medium was used as a HDPE control. Both were used in Examples 3-5 to further verify the strain's potential to degrade plastics.

[0056] The preparation of PDM medium is as follows: weigh 1g KH2PO4, 3g K2HPO4·3H2O, 0.2g MgSO4, 1g NaCl, 0.01g CaCl2, 0.006g Na2SeO35H2O, 0.008g Na2WO42H2O, 0.5g EDTA, 0.2g 7H2O·Fe2SO4, 0.01g 7H2O·ZnSO4, 0.003g 4H2O·MnCl2, 0.03g H3BO3, 0.02g 6H2O·CoCl2, 0.001g 2H2O·CuCl2, 0.002g 6H2O·NiCl2, 0.003g 2H2O·NaMoO4, 15g agar, mix well, and make up to 1L volume with ultrapure water. After high temperature and high pressure sterilization, add HDPE microplastic particles and mix well, cool and solidify for later use.

[0057] Example 3. Infrared spectroscopy identification of polyethylene degradation by Bosnerella QY2 strain

[0058] The infrared spectrum results are as follows Figure 3 As shown, the HDPE control is at about 2918 cm -1 and 2853cm -1 There are strong double peaks at 1468 cm-1, which belong to the asymmetric and symmetric stretching vibrations of –CH2. The bending or rocking deformation band occurs at about 1468 cm-1. -1 and 718cm -1 These characteristic functional groups of HDPE disappeared after being treated with plastic-degrading bacteria Bosnia sp. QY2, indicating that there was no HDPE plastic in the degradation system and that plastic-degrading bacteria Bosnia sp. QY2 could effectively reduce the content of HDPE.

[0059] Example 4. Pyrolysis chromatography-mass spectrometry identification of polyethylene degradation by Bosnerella strain QY2

[0060] The results of pyrolysis chromatography mass spectrometry are as follows Figure 4 As shown, the HDPE control produced a series of triplet peaks consisting of n-alkadienes (dienes), n-alkenes (olefins) and n-alkanes (alkanes). Among them, n-alkenes is the largest of the three peaks. These peaks are almost undetectable after treatment with Bosnia QY2, indicating that HDPE is almost non-existent in the treatment system with added degrading bacteria, and the degrading bacteria Bosnia QY2 strain is able to degrade polyethylene plastics.

[0061] Example 5. Differential Scanning Calorimetry Identification of Bosnerella QY2 Strain

[0062] Figure 5 The peak changes during the heating and cooling scans of the HDPE control and the treatments with added plastic and degrading bacteria are shown, characterizing the effects of plastic degrading bacteria on thermal properties such as crystallization and melting of the HDPE matrix. The DSC curve of the HDPE control crystallizes at ~100°C and melts at ~125°C. The crystallization and melting temperatures of the treatment with the addition of the Bosnerella QY2 strain changed significantly, indicating that the Bosnerella QY2 strain will change the thermodynamic properties of HDPE plastics, and the Bosnerella QY2 strain has a certain ability to utilize HDPE plastics.

[0063] In summary, this application obtained a strain capable of degrading polyethylene by screening the soil of the contaminated site. After 16SrDNA sequencing and identification, it was found that the strain belonged to the genus Bosea (Bosea sp.) strain, and it was named Bosea QY2 strain. Through functional assays, it was found that the Bosea QY2 strain could tolerate 1000 mg / L polyethylene plastic, reduce or eliminate the characteristic functional groups and cracking characteristic substances of polyethylene plastic, and change the thermodynamic properties, indicating that the Bosea QY2 strain has a certain ability to utilize plastic. The discovery of this strain provides new ideas and methods for degrading polyethylene plastics in various environments, which is beneficial to the control of polyethylene plastic pollution and human health.

[0064] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations on the present application. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present application.

Claims

1. A strain of the genus Bosella, characterized in that The Bostonia strain is the Bostonia QY2 (Boseasp.QY2) strain, and its deposit number is CCTCC NO: M 20242096.

2. A bacterial agent comprising the Bosidiolum QY2 strain according to claim 1.

3. The bacterial agent according to claim 2, characterized in that The bacterial agent comprises live bacteria of the Bosnerella QY2 strain.

4. The bacterial agent according to claim 2 or 3, characterized in that The bacterial agent further comprises one or more other active ingredients that can act synergistically with the Bosnerella QY2 strain.

5. Use of the Bosnerella QY2 strain according to claim 1 or the bacterial agent according to any one of claims 2 to 4 in degrading polyethylene.

6. The use according to claim 5, characterized in that The degradation is that the Bosnerella QY2 strain grows with polyethylene as the only carbon source and gradually degrades polyethylene.

7. The use according to claim 5 or 6, characterized in that The polyethylene is high-density polyethylene.

8. The use according to any one of claims 5 to 7, characterized in that: The polyethylene is polyethylene particles, films or powders.

9. The use according to any one of claims 5 to 8, characterized in that The polyethylene was degraded by the Bosnerella QY2 strain in different water bodies.

10. A method for degrading polyethylene, characterized in that: The Bosnerella QY2 strain according to claim 1 or the bacterial agent according to any one of claims 2 to 4 is co-cultured with polyethylene.

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