Preparation and use method of biological agent for purifying heavy metals in aquatic products

Abstract

The invention relates to an optimal preparation of a lactic acid bacteria combined biological agent capable of adsorbing toxic heavy metals such as lead, cadmium and mercury in aquatic products such as shellfish and sea cucumbers, and a preparation process and a use method of the lactic acid bacteria combined biological agent. The method comprises the following steps: optimizing and constructing lactic acid bacteria capable of enriching harmful metals, fermenting and proliferating by using a special culture medium, preparing a biological combined stock solution, and preparing a medium diluent at the same time. Before an aquatic product leaves a factory, a proper amount of water is injected into a heavy metal purification pool (or a transfer cart or a container). And mixing the biological combined stock solution with the medium diluent to prepare a purification solution, injecting the purification solution into a purification tank, uniformly mixing, putting the aquatic product, and carrying out heavy metal adsorption purification.

Description

Technical Field

[0001] The present invention relates to an optimized combination of lactic acid bacteria biological preparations capable of absorbing toxic heavy metals such as lead, cadmium and mercury in aquatic products such as shellfish and sea cucumbers, as well as a preparation process and a method of use thereof. That is, lactic acid bacteria capable of enriching harmful metals are selected and constructed, and after fermentation and proliferation with a special culture medium, a biological combination stock solution is prepared, and a medium dilution liquid is prepared at the same time. Before the aquatic products leave the factory, a heavy metal purification tank (or a transport vehicle, a container) is injected with an appropriate amount of water. The biological combination stock solution is mixed with the medium dilution liquid to prepare a purified liquid, which is then injected into the purification tank, mixed evenly, and then put into the aquatic product for heavy metal adsorption and purification. Background Art

[0002] Heavy metals refer to metals with a density greater than 4.5 g / cm 3 Metals mainly refer to elements with significant biological toxicity such as lead, cadmium, and mercury. With the rapid development of industry, heavy metal pollution is becoming increasingly serious. They enter the human body through air, diet, and skin, and accumulate continuously, causing chronic latent poisoning. Harming health and shortening life span. Heavy metals can replace essential metal elements such as calcium, magnesium, iron, and zinc in the human body, destroying the structure and function of proteins and enzymes. For example, lead in the blood replaces hemoglobin iron, which reduces the oxygen-carrying capacity and makes the human body prone to fatigue; lead occupies the sulfhydryl group on the protein, which reduces its antioxidant function, accelerates aging, reduces the glucose-to-protein function, and causes blood sugar metabolism disorders, etc. More than 90% of the lead accumulated in the body accumulates in the bone marrow, causing chronic cumulative poisoning of stem cells, resulting in low functions such as hematopoiesis, bone formation, regeneration, differentiation, self-healing, and immunity, premature aging of the human body, frequent chronic diseases, and shortened life span.

[0003] The "lead-loving bacteria (Genebank KM233626)" we isolated has strong lead adsorption properties. Xu Ying et al. found that Bifidobacterium ( Bifidobacteria , BB) and Lactobacillus rhamnosus ( Lactobacillusrhamnosus, The adsorption rates of lead by Lactobacillus plantarum (LR) were 97.44% and 96.87% respectively. Lactobacillus plantarum CCFM8610) has a strong tolerance to cadmium and can tolerate 1000mg / L of cadmium. Jadán-Piedra et al. showed that under the premise of simulating gastrointestinal digestion, Lactobacillus casei BL23 has excellent adsorption capacity for mercury in food and water, with adsorption rates reaching 72% to 98% and 74% to 97% respectively. We optimized the formula and enriched the bacteria to prepare the biological combination stock solution.

[0004] Lactic acid bacteria enrich heavy metals and have high requirements for environmental conditions such as water temperature and pH value. Only under the best conditions can its adsorption rate reach a high level. Therefore, preparing a suitable medium is one of the key technologies to ensure the adsorption rate of lactic acid bacteria. Summary of the invention

[0005] The present invention relates to an optimized combination of lactic acid bacteria biological preparations capable of absorbing toxic heavy metals such as lead, cadmium and mercury in aquatic products such as shellfish and sea cucumbers, as well as a preparation process and a method of use thereof. That is, lactic acid bacteria capable of enriching harmful metals are selected and constructed, and after fermentation and proliferation with a special culture medium, a biological combination stock solution is prepared, and a medium dilution liquid is prepared at the same time. Before the aquatic products leave the factory, a heavy metal purification tank (or a transport vehicle, a container) is injected with an appropriate amount of water. The biological combination stock solution is mixed with the medium dilution liquid to prepare a purified liquid, which is then injected into the purification tank, mixed evenly, and then put into the aquatic product for heavy metal adsorption and purification.

Claims

1. Claim 1: Lactobacillus ferrugineus (Genebank KM233626) is inoculated into liquid MRS medium and cultured at 37°C for 36-48h; Bifidobacterium ( Bifidobacteria , BB) and Lactobacillus rhamnosus ( Lactobacillus rhamnosus, LR) inoculated with liquid MRS medium supplemented with 5% tomato juice and cultured anaerobically at 37°C for 36-48h; Lactobacillus plantarum ( Lactobacillus plantarum CCFM8610) and Lactobacillus casei BL23 were inoculated into liquid MRS medium and cultured at 37°C for 36-48 hours. After the culture was completed, the mixture was mixed in proportion to prepare a high-concentration bacterial fermentation liquid.

2. According to claim 2, add an appropriate amount of purified water according to the ratio of 0.3-0.6% citric acid, 1-2% chitosan oligosaccharide, 0.5-1.5% sodium alginate, and 0.5-1% disodium EDTA, and continue stirring for 30-60 minutes to fully dissolve and disperse them to prepare a biological synergist mixed solution.

3. According to claim 3, the bacterial fermentation liquid and the biological enhancement auxiliary agent are mixed evenly in a ratio of 2:1 to prepare a biological combination stock solution.

4. Claim 4: Dipotassium hydrogen phosphate and potassium dihydrogen phosphate are added in an amount of 0.5%-1% to prepare a medium dilution solution.

5. According to claim 5, the biological combination stock solution is mixed with the medium dilution solution, and the mixture is evenly sprinkled into the water body at a ratio of 1:1000, and the aerator is turned on to fully disperse the biological agent and act for 1-2 days; for aquatic products that have been contaminated by heavy metals, the aquatic products can be immersed in the medium dilution solution at a ratio of 1:100 for 30-60 minutes and then rinsed with clean water.