Extract solution comprising nymphaea coerulea flower extract and Chinese lotus flower extract, composition and method thereof

Abstract

Various aspects of the disclosure relate to a cosmetic composition and an associated method, the cosmetic composition comprising an extract solution comprising a flower extract of Nymphaea coerulea (Nymphaea coerulea) and a flower extract of Nelumbo nucifera (Nelumbo nucifera), the cosmetic composition comprising an extract solution comprising a flower extract of Nymphaea coerulea (Nymphaea coerulea (Nymphaea coerulea) and a flower extract of Nelumbo nucifera (Nelumbo nucifera).

Description

[0001] Cross-reference to related applications

[0002] This application claims the benefit of priority under 35 U.S.C. §119(e) to U.S. Provisional Patent Application Serial No. 63 / 368,855 filed on July 19, 2022, U.S. Provisional Patent Application Serial No. 63 / 500,382 filed on May 5, 2023, and U.S. Provisional Patent Application Serial No. 63 / 500,388 filed on May 5, 2023, the disclosures of which are incorporated herein by reference in their entirety. Background Art

[0003] For conventional cosmetics, users usually have to choose between products that can be easily removed and products that remain on the skin for a long time (e.g., all day). Although products that can be easily removed can be quickly removed by, for example, washing with soap and water, users may find themselves repeatedly reapplying products throughout the day because the products may also be accidentally removed due to sweat and contact with clothes, etc. On the other hand, conventional long-lasting cosmetics are less accidentally removed, but may be difficult to wash off, and may require the use of organic solvents or other irritating cosmetic removers. Therefore, there is a continuous need in the art for cosmetic compositions that can be easily removed by washing with soap and water while remaining on the skin for a long period of time. Transfer-resistant cosmetics usually use film-forming polymers to provide a long-lasting film for the skin, lips, hair or eyelashes, and help to spread and adhere the formulation to the surface. Such film-forming polymers can include polymers, such as organosiloxanes, including polydimethylsiloxane (PDMS or dimethicone), which are used due to their excellent diffusion characteristics and biological inertness. Recently, the properties of silicone polymers have been changed by copolymerization with other polymers such as polyurethanes, ethylenically unsaturated monomers or polymers thereof. For example, in U.S. Patent Publication No. 2008 / 0019932, the disclosure of which is hereby incorporated by reference, Revlon describes a color cosmetic composition comprising "at least one silicone film-forming polymer, at least one pigment, and at least one dispersant that aids in dispersing the pigment and the silicone film-forming polymer in the composition."

[0004] Aesthetic conditions of the skin are often associated with natural or environmental causes, such as the aging process (i.e., intrinsic aging), exposure to sunlight or UV radiation therefrom (i.e., extrinsic aging), etc. Other factors, such as improper care and / or diet, stress, nutritional deficiencies, repeated facial movements, and genetic predisposition, also contribute to the development of adverse skin conditions, such as fine lines, frown lines, folds, furrows, and wrinkles.

[0005] Skin aging and wrinkling are multifactorial processes. The formation of fine lines and wrinkles can be attributed to both extrinsic and intrinsic causes. Extrinsic causes include exposure to UV radiation and unquenched reactive oxygen species, smog and other pollutants. Intrinsic factors include degradation of the skin matrix, volume loss (fat and bone) and muscle tone.

[0006] With every smile, squint, laugh or frown, the contraction of facial muscles causes dynamic wrinkles to appear on the face. In chronologically younger skin, these fine lines quickly fade and are not prominent. However, with age, fine lines become deeper and appear more obvious. Most commonly, they appear as deep furrows between the eyebrows ("frown lines"), on the sides of the eyes ("crow's feet") and on the forehead ("glabellar lines").

[0007] The biochemistry and biomechanics of skeletal muscle contraction are well characterized. Contraction is initiated by an electrical signal (action potential) transmitted by a motor neuron. At the neuromuscular junction, the action potential causes vesicles to release the neurotransmitter acetylcholine ("Ach"), which then diffuses across the synapse between the nerve ending and the sarcolemma, i.e., the membrane of the muscle fiber. ACh binds to the ACh receptor, triggering a diffuse action potential and causing the release of calcium ions.

[0008] Muscle groups differ in their sensitivity to muscle relaxants. The muscles in the periorbital and glabellar regions are particularly sensitive to ACh and are therefore susceptible to effective treatment by agents that block ACh release. See, for example, a review article published by L. Vimal and O. Oladapo, "Pharmacology of Non-Depolarising Muscle Relaxants," Issue 5, Article 7, Update in Anaesthesia, World Journal of Anesthesiology Electronic Education and World Federation of Societies of Anaesthesiologists. http: / / www.nda.ox.ac.uk / wfsa / html / u05 / u05_012.htm, accessed on June 20, 2007.

[0009] Botulinum toxin (also known as Allergen, Irvine, Calif., is currently popular for the treatment of wrinkles and fine lines, but was originally used to treat spasticity. This toxin acts on muscle spasticity by specifically inhibiting neurotransmission in nerve cells, thereby causing relaxation of contracted muscles (e.g., A. Blitzer et al., 1993, Arch. Otolaryngol. Head Neck Surg., 119: 118-122; U.S. Pat. No. 6,344,461 B1 to L. Breton et al.). When injected subcutaneously, botulinum toxin can also act on glabellar wrinkles (wrinkles between the eyebrows). (See JD Carruthers, 1992, J. Dermatol. Surg. Oncol., 18: 17-21; U.S. Pat. No. 6,344,461 B1 to L. Breton et al.). However, the full extent of adverse effects associated with long-term use of botulinum toxins and products or treatments containing such materials remains incompletely determined. Botulinum toxin treatment is associated with a number of side effects, including transient fatigue, difficulty swallowing, neck weakness, hoarseness, and localized pain. In addition, many patients who initially respond to botulinum toxin subsequently do not respond to treatment. (See, e.g., published U.S. Patent Application No. US2002 / 00812914 to Hawrot).

[0010] New ingredients for use as effective active agents in compositions and formulations for application, preferably topically, to the skin are of benefit to the industry and consumers for a variety of uses in the fields of skin care and therapy as well as personal use products. Nelupure (INCI: Propylene Glycol (and) Glycerin (and) Egyptian Blue Nymphaea Extract (and) Chinese Lotus Extract, used interchangeably throughout) is a blended extract of two lotus flowers: Egyptian Lotus (Egyptian Blue Nymphaea) and Holy Lotus (Chinese Lotus). It has been postulated that Nelupure (INCI: Propylene Glycol (and) Glycerin (and) Egyptian Blue Nymphaea Extract (and) Chinese Lotus Extract) regulates the sebaceous glands through leptin-receptor inhibition, preventing excess sebum production and inflammatory processes that cause skin blemishes.

[0011] Unwind Sacred Lotus, sold by the Naolys company, is a material made up of active cells from the Chinese lotus plant, and its effects are described as "regulating the skin's nervous system. The Sacred Lotus cells have a relaxing effect that helps restore the skin's original balance." https: / / www.unifect.com / products / unwind-sacred-lotus / BRIEF DESCRIPTION OF THE DRAWINGS

[0012] The drawings generally illustrate various aspects of the disclosure by way of example and not limitation.

[0013] Figure 1 is a flow chart of a scheme used in conjunction with Example 3. Summary of the invention

[0014] The various aspects disclosed relate to a cosmetic composition comprising any of the components disclosed herein. The present disclosure provides compositions and methods comprising newly discovered compositions derived from the lotus plant that are effective in treating (including preventing, reducing, improving and / or eliminating) signs and consequences of dermatological aging of the skin, particularly wrinkles and fine lines, and / or improving the aesthetic appearance of the skin. In addition, these compositions are provided for use in compositions and methods to treat, including preventing, improving and / or reducing hyperhidrosis (or sweating or perspiration) and its related effects, such as malodor. More specifically, the active composition (also referred to herein as an active agent, component, ingredient, agent or compound) is an extract of lotus and / or a blend of extracts of various species of the lotus family, such as, but not limited to, Nelupure (INCI: Propylene Glycol (and) Glycerin (and) Egyptian Blue Water Lily Extract (and) Chinese Lotus Extract) obtained from plants of the Nelumbo family, etc. These botanical materials have recently been found to be useful for treating wrinkles, fine lines and other signs of dermatological aging (i.e., intrinsic aging) or skin exposure to sunlight (i.e., extrinsic aging), and for treating hyperhidrosis because the botanical materials act on muscle cells and nerve cells and tissues at neuromuscular junctions (NMJs) and sweat gland fibers to inhibit acetylcholine release, for example at the NMJs, thereby relaxing or decontracting muscles, such as the muscles of facial expression.

[0015] It has been found that incorporating active ingredients that can reduce epidermal sebum production can result in a cosmetic composition that can remain on the skin for a long period of time. The present disclosure provides a long-acting composition comprising an active ingredient. The active ingredient may include a material that inhibits epidermal sebum production. On the one hand, the active ingredient downregulates epidermal sebum production by leptin receptor inhibition. On the other hand, the active ingredient downregulates epidermal sebum production by competitively inhibiting cell surface leptin receptor activity. On the other hand, the active ingredient downregulates epidermal sebum production by downregulating nicotinic acetylcholine receptors (nAchR). On the other hand, the active ingredient downregulates epidermal sebum production by competitively inhibiting nAchR activity. On the other hand, the active ingredient inhibits acetylcholinesterase activity; generation; and / or stability. On the other hand, the active ingredient inhibits acetylcholine release.

[0016] In certain aspects, the skin in need of the disclosed composition can be skin with excess sebum. In further aspects of the method for treating skin with excess sebum, the skin benefit can be selected from the group consisting of: restoring matte texture of the skin; evening skin type; reducing oiliness / greasy feel of skin and / or hair; reducing the occurrence of dandruff; reducing the occurrence of clogged / blocked pores; reducing the occurrence of acne; reducing the occurrence of acne lesions; reducing areas of lipid overexpression or overproduction; reducing areas of lipid overexpression or overproduction; reducing the thickness of skin affected by lipid overproduction; and / or combinations thereof.

[0017] Another aspect of the disclosure provides compositions, formulations and methods containing materials newly identified as useful for treating wrinkles, fine lines, folds, furrows and other signs of aging skin, in addition to treating, preventing or reducing hyperhidrosis. According to the disclosure, these materials exert their effectiveness by acting at the neuromuscular junction (NMJ), such as in the muscle cells and nerve cells or tissue layers at the application site, such as the skin of the face, neck, arms, feet or hands, or in the dermis layer of the skin where sweat glands are located. More specifically, extracts from flowers of one or more species of the lotus composition block or inhibit the release of acetylcholine at the NMJ of skeletal muscle, which in turn causes contracted muscles to relax or decontract. Contracted muscles are associated with the formation of fine lines, wrinkling, etc.

[0018] It should be understood that, as used herein, the terms treating and treatment include and encompass preventing, reducing, ameliorating, improving, alleviating and / or eliminating the dermatological effects of aging and sun exposure, particularly with respect to wrinkles, fine lines, folds, furrows, folds, etc. of the skin. The present disclosure further encompasses treating "marionette" fine lines extending on either side of the mouth as defined above, as well as fine lines on the forehead and vertical fine lines between the eyebrows. The compositions and methods of the present invention are also suitable for treating dermatological conditions in many areas of the body as defined above, including but not limited to the face, forehead, neck, arms, hands, legs, knees, feet, chest, back, groin, buttocks, etc. DETAILED DESCRIPTION

[0019] Reference will now be made in detail to certain aspects of the disclosed subject matter.While the disclosed subject matter will be described in conjunction with the enumerated claims, it will be understood that the illustrated subject matter is not intended to limit the claims to the disclosed subject matter.

[0020] Throughout this document, values ​​expressed in range format should be understood in a flexible manner to include not only the values ​​explicitly stated as the limits of the range but also all individual values ​​or sub-ranges encompassed within the range, as if each value and sub-range were explicitly stated. For example, a range of "about 0.1% to about 5%" or "about 0.1% to 5%" should be understood to include not only about 0.1% to about 5%, but also individual values ​​(e.g., 1%, 2%, 3% and 4%) and sub-ranges (e.g., 0.1% to 0.5%, 1.1% to 2.2%, 3.3% to 4.4%) within the indicated range. Unless otherwise indicated, the statement "about X to Y" has the same meaning as "about X to about Y". Similarly, unless otherwise indicated, the statement "about X, Y or about Z" has the same meaning as "about X, about Y or about Z".

[0021] In this document, unless the context clearly indicates otherwise, the terms "a", "an" or "the" are used to include one or more than one or one or more than one. Unless otherwise indicated, the term "or" is used to refer to a non-exclusive "or". The statement "at least one of A and B" has the same meaning as "A, B, or A and B". In addition, it should be understood that the words or terms used herein and not otherwise defined are for descriptive purposes only and are not limiting. Any use of section headings is to assist in the reading of this document and should not be construed as limiting; information related to the section heading may appear within or outside the particular section being described.

[0022] As used herein, the term "composition" includes compositions intended for application to body surfaces (e.g., skin or hair). The term "composition" may also include cleaning compositions, such as fabric care compositions or home care compositions, including air care compositions (e.g., air fresheners), for use on clothing or other substrates such as hard surfaces (e.g., dishes, floors, countertops). Additional non-limiting examples of "compositions" may also include face or body powders, deodorants, foundations, body / facial oils, mousses, creams (e.g., cold creams), waxes, sunscreens and sunscreens, bath and shower gels, lipsticks, self-tanning compositions, masks, and patches.

[0023] As used herein, the term "consumer" refers to the user of the composition and observers near or around the user.

[0024] It is to be understood that the test methods disclosed in the Test Methods section of this application may be used to determine corresponding values ​​for the parameters of Applicants' disclosure as described and claimed herein.

[0025] In all aspects of the present disclosure, unless the context clearly states otherwise, all percentages are based on the weight of the total composition, which is obvious from the context. Unless otherwise clearly stated, all ratios are weight ratios, and unless otherwise clearly stated, all measurements are made at 25°C.

[0026] The pH of the composition can range from about 4 to about 8, from about 5 to about 7, less than, equal to, or greater than about 4, 5, 6, 7, or about 8. The ethanol concentration in any of the compositions described herein can be less than about 78 wt % ethanol, less than about 70 wt % ethanol, less than about 60 wt % ethanol, less than about 50 wt % ethanol, less than about 40 wt % ethanol, less than about 30 wt % ethanol, less than about 20 wt % ethanol, less than about 10 wt % ethanol, or free of ethanol.

[0027] Epidermis and Dermis

[0028] Human skin contains two main compartments: a superficial outer compartment, i.e., the epidermis, and a deeper compartment, i.e., the dermis. The outermost epidermal skin layer generally provides a certain degree of protection for the body, but fine lines and wrinkles can easily appear in this part of the skin. The epidermis and dermis bear the harmful effects of photodamage. The function of the natural human epidermis is to provide the basic protective role of the skin in the human body. The dermis, which provides solid and nutritional support for the epidermis, mainly contains fibroblasts and an extracellular matrix, which is mainly composed of substances including collagen, elastin and glycosaminoglycans ("GAGs"). In addition, the dermis contains leukocytes, mast cells, tissue macrophages, blood vessels, muscle cells and nerve fibers. The dermal muscles of the face are controlled by motor nerve interference of the facial nerve. The interlobular septa of the hypodermis contain fibers constituting striated muscle tissue, i.e., carrageenan, within it. It is known that a subpopulation of dermal fibroblasts called myofibroblasts have contractile properties similar to the contractile properties of muscle cells and tissues.

[0029] Facial fine lines

[0030] Fine lines between the eyebrows are produced by the activity of the corrugator and orbicularis oculi muscles. These muscles move the eyebrows inward, and the procerus and depressor supercilii pull the eyebrows downward. This produces a frown or "furrowed brow". The location, size and use of the muscles vary significantly between individuals. Fine lines caused by facial expressions occur perpendicular to the direction of action of the contracting facial muscles. The effective dose for facial fine lines is determined by gross observation of the patient's ability to activate the injected superficial muscles.

[0031] Lateral canthal wrinkles originate primarily from the activity of the orbicularis oculi muscle around the eye, which is responsible for blinking and eyelid closure. Forceful contraction of the orbicularis oculi muscle causes laterally and radially oriented wrinkles (crow's feet) originating from the lateral canthus. The distribution of these radial wrinkles varies between patients. Forehead wrinkles are primarily caused by the activity of the frontalis muscle. This muscle moves the eyebrows upward, interacting with the procerus, orbicularis, corrugator, and procerus supercilii muscles. Contraction of the frontalis muscle causes the eyebrows to rise. The location, size, and use of the muscles vary significantly between individuals.

[0032] Hyperseborrheic skin

[0033] Some people exhibit hyperseborrheic skin, characterized by excessive sebum secretion and excretion. Typically, individuals with hyperseborrheic skin exhibit greater than 200 μg / cm2 measured on the forehead. 2 The sebum level of the skin can be increased. This excessive production of sebum can cause aesthetic problems, such as oily / glossy skin or hair, acne-prone skin, enlarged pores, thickened skin, or poor makeup retention. In addition, excess sebum can act as a catalyst for acne by clogging pores, thereby forming comedones ("blackheads" or "whiteheads"), which, when not treated, may become inflamed and progress to acne vulgaris. In addition, odors may be emitted due to the accumulation, production, or excretion of excess sebum. The compositions described herein can provide skin benefits because they can prevent these undesirable effects caused by excessive sebum production.

[0034] In a further aspect, the skin benefit may be selected from the group consisting of: reducing lipid production by sebaceous glands; reducing lipid synthesis in subcutaneous adipose tissue; reducing triglyceride synthesis; reducing fatty acid synthesis; preventing and / or improving skin-related conditions associated with inhibition of lipolysis; preventing and / or improving skin-related conditions associated with non-selective or partially selective PPAR-γ stimulators / up-regulators; preventing and / or improving skin-related conditions associated with non-selective or partially selective up-regulation of lipogenic genes (typically up-regulated by PPAR-γ activation); preventing and / or improving skin-related conditions associated with non-selective or partially selective FAS stimulation. preventing, improving or treating skin conditions associated with non-selective or partially selective SCD-1 receptor stimulators / upregulators; preventing and / or ameliorating skin conditions associated with non-selective or partially selective SCD-1 receptor stimulators / upregulators; preventing, improving or treating oily skin; preventing, improving or treating oily hair; preventing, improving or treating oily scalp; preventing, improving or treating enlarged pores; preventing, improving or treating acne-prone skin; preventing, improving or treating body odor associated with excessive sebum production; preventing, improving or treating cellulite in adults; preventing, improving or treating excessive accumulation or production of subcutaneous fat in adults; improving skin texture associated with cellulite; and / or combinations thereof.

[0035] In certain aspects, the skin in need of the disclosed composition can be skin with excess sebum. In further aspects of the method for treating skin with excess sebum, the skin benefit can be selected from the group consisting of: restoring matte texture of the skin; evening skin type; reducing oiliness / greasy feel of skin and / or hair; reducing the occurrence of dandruff; reducing the occurrence of clogged / blocked pores; reducing the occurrence of acne; reducing the occurrence of acne lesions; reducing areas of lipid overexpression or overproduction; reducing areas of lipid overexpression or overproduction; reducing the thickness of skin affected by lipid overproduction; and / or combinations thereof.

[0036] According to various aspects of the present disclosure, the cosmetic composition may include an active component.

[0037] Acetylcholine and acetylcholine release inhibitors

[0038] The neurotransmitter acetylcholine is secreted by neurons in many areas of the brain, but specifically by the large pyramidal cells of the motor cortex, by several different neurons in the basal ganglia, by the motor neurons that control skeletal muscles, by the preganglionic neurons of the autonomic nervous system (both sympathetic and parasympathetic), by the bag 1 fibers of the muscle spindle fibers, by the postganglionic neurons of the parasympathetic nervous system, and by some postganglionic neurons of the sympathetic nervous system. In essence, only the postganglionic sympathetic nerve fibers to the sweat glands, the erector pili muscles, and several blood vessels are cholinergic, because most of the postganglionic neurons of the sympathetic nervous system secrete the neurotransmitter norepinephrine. In most cases, acetylcholine has an excitatory effect. However, it is known that acetylcholine has an inhibitory effect on some peripheral parasympathetic nerve endings, such as suppressing heart rate by the vagus nerve.

[0039] The outgoing signals of the autonomic nervous system are transmitted to the body through the sympathetic nervous system or the parasympathetic nervous system. The preganglionic neurons of the sympathetic nervous system extend from the preganglionic sympathetic neuron cell bodies located in the intermediolateral horn of the spinal cord. The preganglionic sympathetic nerve fibers extending from the cell bodies synapse with postganglionic neurons located in the paravertebral sympathetic ganglia or prevertebral ganglia. Since the preganglionic neurons of both the sympathetic and parasympathetic nervous systems are cholinergic, applying acetylcholine to the ganglia will excite the sympathetic and parasympathetic postganglionic neurons.

[0040] Acetylcholine activates two types of receptors: muscarinic receptors and nicotinic receptors. Muscarinic receptors are present in all effector cells stimulated by postganglionic neurons of the parasympathetic nervous system and in effector cells stimulated by postganglionic cholinergic neurons of the sympathetic nervous system. Nicotinic receptors are present in the adrenal medulla and in the autonomic ganglia, that is, on the cell surface of postganglionic neurons at the synapses between preganglionic neurons and postganglionic neurons of both the sympathetic and parasympathetic nervous systems. Nicotinic receptors are also present in many non-autonomic nerve endings, such as in the membranes of skeletal muscle fibers at the neuromuscular junction.

[0041] Acetylcholine is released from cholinergic neurons when small, transparent intracellular vesicles fuse with the presynaptic neuron cell membrane. Various non-neuronal secretory cells, such as the adrenal medulla (and PC12 cell line) and pancreatic islet cells, release catecholamines and parathyroid hormone, respectively, from large, dense-core vesicles. The PC12 cell line is a clone of rat pheochromocytoma cells that is widely used as a tissue culture model for studying sympathetic adrenal development.

[0042] The neuromuscular junction is formed in skeletal muscle by the proximity of the axon to the muscle cell. Signals transmitted through the nervous system generate action potentials at the terminal axons, where ion channels activate and cause the neurotransmitter acetylcholine to be released from synaptic vesicles within the neuron, such as at the motor endplate of the neuromuscular junction. Acetylcholine travels through the extracellular space to bind to acetylcholine receptor proteins on the surface of the muscle endplate. Once sufficient binding occurs, the action potential of the muscle cell causes specific membrane ion channel changes, causing the muscle cell to contract. Acetylcholine is then released from the muscle cell and metabolized by cholinesterase in the extracellular space. The metabolites are recycled back into the terminal axons to be reprocessed into additional acetylcholine.

[0043] Sebum and sweat reduction and proposed mechanism of action (leptin receptor downregulation / inhibition of acetylcholine release)

[0044] It has been found that incorporating active ingredients that can reduce epidermal sebum production can result in a cosmetic composition that can remain on the skin for a long period of time. The present disclosure provides a cosmetic composition comprising an active ingredient. The active ingredient can include a material that inhibits epidermal sebum production. In one aspect, it has been proposed that the active ingredient downregulates epidermal sebum production by downregulating leptin receptors. In another aspect, the active ingredient downregulates epidermal sebum production by competitively inhibiting cell surface leptin receptor activity. In another aspect, the active ingredient inhibits acetylcholine release. The active substance does not downregulate sebum via ach receptors. It was found that the active substance inhibits acetylcholine release.

[0045] Mechanism of action

[0046] In one aspect, the active ingredient is a leptin receptor down-regulator and / or an acetylcholine release inhibitor. In one aspect, the leptin receptor down-regulator and / or the acetylcholine release inhibitor is an extract solution that may include an Egyptian blue water lily extract and a Chinese lotus extract. In one aspect, the solution is available as a product under the trade name NELUPURE (INCI: Propylene glycol (and) glycerol (and) Egyptian blue water lily extract (and) Chinese lotus extract). Without intending to be bound by any theory, it is believed that the extract solution can be used in a cosmetic composition in part because the cosmetic composition reduces the expression of leptin cell receptors in sebaceous gland cells, thereby preventing the inflammatory process that triggers sebum production and causes skin spots; and / or inhibits the release of acetylcholine at the neuromuscular junction. It has been shown that leptin, a protein synthesized and secreted by different types of cells in the body (including skin cells), plays a role in the cellular mechanism that triggers sebum synthesis. The benefit of including an extract solution is at least to some extent unexpected because the extract solution can be an inhibitor of acetylcholine release. According to some aspects, the ability of the extract solution to serve as a component of a cosmetic composition is determined by assays for determining sebum levels; leptin cell surface receptors in sebocytes; and / or acetylcholine release inhibition in a sample that has been contacted with the extract or a composition comprising the extract.

[0047] The ability of extract solutions of Egyptian blue water lily extract and Chinese lotus extract to provide beneficial properties as acetylcholine release inhibitors suggests that other natural extracts having acetylcholine release inhibitory properties may also be used in the manner described herein.

[0048] According to some aspects, the ability of the extract solution to serve as a component of a cosmetic composition is determined by an assay (eg, an ELISA assay) for determining acetylcholine levels in a sample that has been contacted with the extract or a composition comprising the extract.

[0049] The present disclosure provides novel compositions and methods comprising newly discovered components that are effective in treating; and / or improving the aesthetic appearance of the skin, including preventing, reducing, ameliorating, inhibiting, alleviating and / or eliminating signs and consequences associated with dermatological aging of the skin, particularly wrinkles, folds, furrows, folds and fine lines, due to chronological and / or hormonal aging or due to light exposure of the skin (e.g., sunlight or ultraviolet radiation). Further, these components are provided for use in compositions and methods to treat, including preventing, ameliorating, inhibiting and / or reducing hyperhidrosis (sweating or perspiration) and its related effects, such as malodor and non-aesthetic appearance.

[0050] More specifically, the components of the present disclosure (also referred to herein as active agents, constituents, ingredients, agents, substances or compounds) are derived from flower extracts of one or more species of lotus, obtained from plants and the like, preferably the Nelumbo family. These plant materials have been newly identified as effective components in compositions and methods for treating, preventing, reducing, inhibiting, ameliorating and / or eliminating wrinkles, fine lines, folds, folds, furrows and other signs of dermatological aging due to chronological and / or hormonal aging or due to sun exposure of the skin, and for treating hyperhidrosis.

[0051] According to the present disclosure, but not wishing to be bound by theory, flower extracts of one or more species of lotus composition exert their effects through their ability to inhibit, block, reduce or prevent the release of acetylcholine at the neuromuscular junction (NMJ) of skeletal muscle cells and tissues, thereby relaxing muscles, such as those associated with facial movements or expressions. Both nerve cells and muscle cells (skeletal muscle cells) are electrically excitable, and the junctions between these types of cells are referred to as NMJs. It has been known for many years that stimulation of the motor nerves that innervate skeletal muscle also causes the release of acetylcholine, and acetylcholine in turn stimulates skeletal muscle contraction. Therefore, acetylcholine has been identified as a neurotransmitter at the neuromuscular junction. (See, e.g., B. Alberts et al., 1989, Molecular Biology of the Cell, 2nd edition, Chapter 19, The Nervous System, Garland Publishing, Inc., NY., p. 1075, etc.). The present disclosure provides flowers of one or more species of the Lotus composition that can inhibit, reduce, block or prevent the release of acetylcholine at the NMJ of skeletal muscle, and thus relax the muscles, preferably muscles associated with facial expression or movement, particularly when used in the compositions and methods of the present invention. Compositions and methods comprising flower extracts from one or more species of the Lotus composition as described herein can reduce, treat and / or prevent excessive sweating or perspiration by inhibiting acetylcholine release.

[0052] The flower extracts of one or more species of the lotus composition cause the modulation of motor contraction through the ability of muscle cells to inhibit the release of acetylcholine so as to relax the muscle fibers in the skin or subcutaneous muscle and / or nerve tissue, thereby reducing wrinkles and fine lines, folds, furrows, etc. By relaxing or preventing the contraction or excessive contraction of the skin or subcutaneous muscle cells in areas such as the face or hands, feet, etc., the flower extracts of one or more species of the lotus and the compositions containing the flower extracts of one or more species of the lotus can effectively smooth the appearance of the skin in these areas, where muscle contraction can lead to the formation of wrinkles, etc. Therefore, if muscle cell contraction is associated with the formation of wrinkles, fine lines, etc., the relaxation or decontraction of the contraction of the skin or subcutaneous muscle tissue by the flower extracts of one or more species of the lotus actives in the compositions of the present disclosure can loosen or relax the contracted muscle tissue and alleviate, reduce, improve, inhibit or eradicate wrinkles, fine lines, etc. The contraction of the skin or subcutaneous muscle cells or skin tissue can cause wrinkling, fine lines, etc., which constitute at least some of the visible dermatological effects of aging due to time and / or hormone aging and / or due to light exposure, as described herein.

[0053] Thus, in one of its aspects, the present disclosure encompasses compositions, formulations and methods containing components newly identified as useful for treating wrinkles, fine lines, folds, furrows and other signs of aging and / or skin photoexposure, in addition to treating, preventing or reducing hyperhidrosis, preferably flower extracts of one or more species of the Lotus composition. Skin in various areas of the body is suitable for treatment and / or receiving the compositions of the present disclosure, face, forehead, neck, arms, legs, hands, feet, torso (chest), back, etc.

[0054] Plant-derived materials from the lotus family according to the present disclosure exert their effects by acting preferentially at the neuromuscular junction (NMJ), such as in and around muscle cells and nerve cells and tissues at the site of application, such as the skin of the face, neck, arms, feet, hands or chest, or in the dermis of the skin where sweat glands are located. Heretofore, it was not previously known or recognized that a combination of materials could inhibit the release of acetylcholine at the NMJ of skeletal muscle, thereby relaxing muscles associated with aesthetically unpleasant wrinkles, frown lines, fine lines, folds, furrows, folds, neck bands, etc. (which may be due to chronological and / or hormonal aging, prolonged or excessive exposure of the skin to sunlight, ultraviolet radiation associated with light exposure, or aging caused by over-exercising facial muscles).

[0055] Generally, for the purposes of this disclosure, a substance is considered a muscle relaxant when it causes a relaxation effect on contracted muscle cells or tissues, such as skin or subcutaneous muscle tissue, such as the flower extracts of one or more species of the lotus composition of the described composition, and / or exhibits an inhibitory effect on acetylcholine release at the NMJ. Contracted muscle cells or tissues are associated with the formation of wrinkles, fine lines, etc. Contracted muscle relaxation or deconstriction, such as by the action of the flower extracts of one or more species of the lotus to inhibit or block acetylcholine release at the NMJ, is used to smooth the appearance of the skin or micro-relief, thereby preventing, ameliorating, reducing and / or eradicating the formation of wrinkles and fine lines, etc., caused by contracted muscle tissue of the skin.

[0056] For use in the compositions of the present disclosure, flower extracts of one or more species of lotus extracts can be chemically synthesized. Alternatively, these plant alkaloids can be extracted from plants. When extracted, the plant alkaloids can be in pure form, semi-purified form, or can be components of unpurified plant extracts.

[0057] Flower extracts of one or more species of lotus plant materials contained in the compositions of the present disclosure can be chemically synthesized in large quantities on an industrial scale. Alternatively, materials can be extracted from natural raw materials of plants. Any and all preparation methods can be used, including the use of cultured plant seed cells, as disclosed in co-owned patent application U.S. Serial No. 10 / 040,242, which is hereby incorporated by reference herein. For example, unpurified plant extracts can be used in the present disclosure with respect to the solubility of specific plant extracts in water or organic solvents, and the extraction process can be different. Two methods for extracting materials from raw plant materials include organic solvent extraction and aqueous-organic solvent extraction, as described in U.S. Patent No. 6,372,239 to Wu et al. Organic extraction methods involve the steps of continuously washing plant materials and extracting plant materials for organic solvent streams. Examples of organic solvents include, but are not limited to, methanol, ethanol, dichloromethane, chloroform, xylene and petroleum ether. Alternatively, alkaloids can be partially purified or completely purified. Chemical synthesis of alkaloids avoids the need for extraction and purification.

[0058] According to the present disclosure, the flower extracts of one or more species of lotus composition contain compositions, including but not limited to formulations for topical application, antioxidants, anti-inflammatory agents, sunscreens, cosmetics, including makeup, anti-aging formulations, such as creams for fine lines and / or wrinkles, topicals, skin penetration enhancers, antiperspirants, deodorants, etc. According to the present disclosure, the flowers of one or more species of lotus composition, and other ingredients comprising such compositions can be formulated in various product forms. Preferably, the composition is prepared in a targeted delivery system, such as transdermal, such as a patch, etc., particularly for topical application.

[0059] The present disclosure encompasses compositions comprising flower extracts of one or more species of the Lotus composition, preferably in a pharmaceutically acceptable cosmetic or dermatological formulation suitable for contact with living animal tissue, including human tissue, and suitable for topical application with little adverse physiological effect, such as irritation, to the user. Thus, the compositions of the present invention are particularly suitable for sensitive skin.

[0060] Compositions encompassed by the present disclosure can provide in any cosmetic and / or dermatologically suitable form, preferably as lotion, gel, emulsifiable paste, or be incorporated in a transdermal patch, and also with ointment or oil base, and sprayable liquid form is provided. For other suitable cosmetic forms of compositions disclosed herein, for example emulsion, mousse, lip balm, lip gloss, lotion, facial mask, ointment, hair cream, solution, essence, spray or tissue. In addition, the compositions considered by the present invention can include conventional and known more compatible cosmetics acceptable adjuvants of skilled practitioners, such as coloring agents, aromatics, emollients, wetting agents, preservatives, vitamins, chelating agents, thickening agents, etc., and other botanicals, such as aloe, chamomile, etc., and as further described below. Compositions are preferably topically applied once or twice every day. Applying daily can continue up to two weeks, four weeks or longer time periods.

[0061] In one aspect of covering sunscreen formulations, the composition comprising flower extracts of one or more species of the lotus composition can further include, but is not limited to, ingredients such as avobenzone, cinnamic acid derivatives (e.g., octyl methoxycinnamate), octyl salicylate, oxybenzone, titanium oxide, zinc oxide, and mixtures or combinations thereof. Such formulations may also preferably include alpha hydroxy acids, oxa acids, oxadiacids, and mixtures or combinations thereof.

[0062] The compositions of the present disclosure may also be formulated into liposomes, which may contain other additives or substances, and / or may be modified to more specifically reach a certain site or remain at a certain site after administration. Alternatively, compositions containing flower extracts of one or more species of lotus may be injected subcutaneously (sc) or intradermally (id) (e.g., by microneedle therapy, optionally in combination with a transdermal patch) at sites where it is desired to reduce, improve, prevent and / or eliminate wrinkles, fine lines, etc., due to the inhibition of the release of acetylcholine at the NMJ and the accompanying relaxation of the contracted muscles.

[0063] Pharmaceutical or physiologically acceptable compositions suitable for use in the present disclosure include compositions in which active ingredients, components or materials are contained in an amount effective to achieve the intended purpose. For example, in the composition of the present invention, flower extracts of one or more species of lotus composition are present in an amount of about 0.0001wt% to about 10wt% based on the total weight of the composition. More preferably, the composition of the present invention includes one or more flower extracts of one or more species of lotus composition in an amount of about 0.0005wt% to about 5wt%. Most preferably, the composition of the present invention includes one or more flower extracts of one or more species of lotus composition in an amount of about 0.001wt% to about 2wt% of the total composition.

[0064] The determination of an effective dose or amount is well within the capabilities of those skilled in the art. A therapeutically effective dose refers to the amount of active ingredient, such as a flower extract of one or more species of lotus composition identified according to the present disclosure, which, for example, treats, prevents, improves, reduces or eliminates a condition, more specifically, wrinkles, fine lines, folds, etc. The exact dose will be determined by a practitioner who will consider factors related to the individual in need of treatment. Dosage and administration are adjusted to provide sufficient levels of active ingredients or to maintain the desired effect. Factors typically considered include the severity of the individual's specific needs, the patient's general health, the individual's age, weight and sex, diet, administration time and frequency, drug combination, reaction sensitivity and tolerance / response to treatment. As a general guide, a long-lasting pharmaceutical composition can be administered daily, every 2 to 4 days, weekly, or biweekly, depending on the half-life and clearance rate of the particular formulation. Variations in these dosage levels can be adjusted using standard empirical methods well known in the art for optimization. Guidance on specific doses and delivery methods is provided in the literature and is generally available to practitioners in the art. Those skilled in the art will adopt different formulations according to the properties, such as structure and composition, of the active extracts of the flowers of one or more species constituting the lotus.

[0065] The compositions of the present disclosure improve the aesthetic appearance of the skin by treating at least one of: dermatological aging, particularly signs of chronological, actinic or hormonal aging, or signs of extrinsic aging, such as sun exposure. In particular, improving the aesthetic appearance of the skin includes at least one of: making facial fine lines appear less noticeable; making facial fine lines and / or wrinkles feel filled; improving the appearance of infraorbital fine lines and / or periorbital fine lines; improving the appearance of crow's feet; reducing and / or attenuating the appearance of wrinkles, particularly wrinkles on the cheeks, forehead (e.g., vertical wrinkles between the eyes, horizontal wrinkles above the eyes) and / or around the mouth (e.g., marionette lines), and particularly deep wrinkles, folds or creases; improving skin flexibility; reducing and / or eliminating fine lines and / or deep fine lines, folds and creases; and smoothing the skin, for example to the extent of reduced wrinkling / fine lines.

[0066] Contemplated by the present disclosure are transdermal delivery modes, such as patches, etc., with or without suitable skin penetration enhancers. The methods and compositions embodied in the present disclosure include such means: one or more flower extracts of one or more species of lotus composition can be effectively applied in a transdermal system. Typically, transdermal delivery is poorly absorbed locally or the desired compound at high dose levels. Therefore, the transdermal means of delivering the composition or formulation (usually with a skin penetration enhancer composition) to the skin is a transdermal means of a transdermal patch or similar device known and described in the art. Examples of such devices are disclosed in U.S. Patents Nos. 5,146,846, 5,223,262, 4,820,724, 4,379,454 and 4,956,171; such descriptions are not intended to be limiting. The transdermal mode of storing and delivering the composition to the skin and forming an active composition is convenient and very suitable for the purpose of one aspect of the present disclosure, using a topical patch, applying the patch to the desired area for a long period of time. Preferably, the long period of time is greater than one hour; more preferably, the long period of time is overnight, for example, while the user is sleeping.

[0067] Particular aspects of the present disclosure relate to delivering the described compositions by using targeted delivery systems, such as liposomes, microspheres (see, e.g., U.S. Pat. No. 5,770,222 to Unger et al.), transdermal patches, etc., so that the flower extracts of one or more species of the Lotus active can more easily reach and affect the muscle layer of the application area, such as the face or neck, or the dermis of the skin where sweat glands are located, such as the underarms, feet, hands, etc. Compositions comprising flower extracts of one or more species of the Lotus composition, including liposomal formulations, can also be administered by direct subcutaneous or intradermal injection to more precisely deposit the active agent at the site where inhibition of muscle relaxation or deconstriction through acetylcholine release is desired.

[0068] Liposomes and delivery systems and vehicles involving liposomes are well known in the art. In short, liposomes are unilamellar or multilamellar lipid vesicles that capture a considerable portion of an aqueous solution. The vesicle microreservoirs of liposomes can contain various water-soluble materials suspended in an emulsion (e.g., reviewed in G. Gregorius (ed.), 1991, Liposome Technology, Vol. I, II, III, CRC Press, Boca Raton, Fla.; Davis SS and Walker IM, 1987, Methods in Enzymology, 149: 51-64; Mayhew E. et al., 1987, Methods in Enzymology, 149: 64-77; and Shafer-Korting M. et al., 1989, J. Am. Acad. Dermatol., 21: 1271-1275). The preparation of liposomes and various uses of liposomes in biological systems have been described (e.g., in U.S. Pat. No. 4,708,861 to MC Popescu et al., U.S. Pat. No. 4,224,179 to M. Schneider et al., and U.S. Pat. No. 4,235,871 to D. P Papahadjopoulos et al.) Thus, such liposomes can be formulated into any dermatological or cosmetic composition as described herein.

[0069] In addition to the flower extract of one or more species of the lotus constituents as active agents, as described herein, the physiologically acceptable and pharmaceutical compositions may also contain suitable pharmaceutically acceptable carriers, diluents or excipients, which include adjuvants that facilitate processing of the active compounds into pharmaceutically usable preparations. Additional details on the techniques of formulation and administration are provided in the latest edition of Remington's Pharmaceutical Sciences (Mack Publishing Co.; Easton, Pa.). Pharmaceutical compositions containing flower extracts of one or more species of the lotus constituents disclosed herein may be manufactured in a manner known in the art, for example, by conventional mixing, dissolving, granulating, dragee-making, grinding, emulsifying, encapsulating, embedding or lyophilizing processes.

[0070] If applicable, the pharmaceutical composition can be provided as a salt and can be formed with many acids, including but not limited to hydrochloric acid, sulfuric acid, acetic acid, lactic acid, tartaric acid, malic acid, succinic acid, etc. Salts are often more soluble in aqueous solvents or other protic solvents than the corresponding free base forms. In other cases, the formulation can be a lyophilized powder.

[0071] Preferred aspects of the topical compositions of the present disclosure further include at least one of: a surface smoothing agent, a dermal filler, an optical diffuser, a sunscreen, an exfoliation enhancer, or an antioxidant.

[0072] Surface smoothing agents provide functional benefits of enhancing skin smoothness and reducing the appearance of fine lines and coarse wrinkles. Examples include, but are not limited to, isopropyl myristate, petrolatum, isopropyl lanolinate, silicones (e.g., methicone, dimethicone), or any mixture thereof. Surface smoothing agents preferably comprise from about 0.1 wt% to about 50 wt% of the total weight of the composition.

[0073] Dermal fillers act as collagen enhancers for the skin. An example of a suitable and preferred dermal filler is palmitoyl oligopeptide. Other non-limiting examples of dermal fillers include collagen and / or glycosaminoglycan (GAG) enhancers. The dermal filler preferably comprises from about 0.1 wt% to about 20 wt% of the total weight of the composition.

[0074] Optical diffusers are particles that change the surface luminosity of the skin, causing, for example, visual blurring and softening of fine lines and wrinkles. Examples of optical diffusers that can be used in the present disclosure include, but are not limited to, boron nitride, mica, nylon, polymethyl methacrylate (PMMA), polyurethane powder, sericite, silica, silicone powder, talc, Teflon, titanium dioxide, zinc oxide, or any mixture thereof. The optical diffuser preferably accounts for about 0.01 wt% to about 20 wt% of the total weight of the composition.

[0075] In another aspect, the present disclosure includes sunscreens that protect the skin from damaging UV rays. Illustratively, the sunscreen provides UVA and UVB protection by using a single sunscreen or a combination of sunscreens. Among the sunscreens that can be used in the compositions of the present invention are avobenzone, cinnamic acid derivatives (such as octyl methoxycinnamate), octyl salicylate, oxybenzone, titanium dioxide, zinc oxide, or any mixture or combination thereof. Preferably, the sunscreen is present in about 1 wt % to about 30 wt % of the total weight of the composition. In particular, the sunscreen is preferably added to prevent / reduce photodegradation of the composition and / or the ingredients therein in the packaging and / or on the skin after application.

[0076] It will be appreciated that the compositions of the present disclosure containing a sunscreen provide additional improvements to the aesthetic appearance of the skin, including at least one of minimizing sunburn, minimizing tanning, reducing redness, and reducing future wrinkle development. It will be appreciated that when the topical composition is intended to be applied before retiring at night, the addition of a sunscreen may not be preferred.

[0077] The composition of the present invention may also have one or more exfoliation promoters. Suitable examples of exfoliation promoters that can be used in the composition of the present invention include keratolytic agents, i.e., active agents having peeling, peeling or scrubbing properties, or active agents that can soften the epidermal stratum corneum of the skin; alpha (alpha) and / or beta (beta) hydroxy acids; benzoyl peroxide; keto acids, such as pyruvic acid, 2-oxopropionic acid, 2-oxobutyric acid and 2-oxopentanoic acid; oxacids, such as those disclosed in U.S. Pat. Nos. 5,847,003 and 5,834,513, the disclosures of which are incorporated herein by reference; urea; retinoids or mixtures thereof. These anti-wrinkle or anti-fine line active agents can be formulated, for example, in an amount of about 0.0001% to 5% by weight relative to the total weight of the composition.

[0078] More specifically, the example of hydroxy acid includes but is not limited to straight chain, branched chain, cyclic, saturated or unsaturated α-hydroxy acid or β-hydroxy acid. The hydrogen atom in the main chain based on carbon of these materials can be replaced by halogen, halogen-containing alkyl, acyl, acyloxy, alkoxycarbonyl or alkoxy with 2 to 18 carbon atoms. Suitable hydroxy acid includes, for example, glycolic acid, lactic acid, malic acid, tartaric acid, citric acid, 2-hydroxyalkanoic acid, mandelic acid, salicylic acid and alkyl derivatives thereof, including 5-n-octanoyl salicylic acid, 5-n-dodecanoyl salicylic acid, 5-n-decanoyl salicylic acid, 5-n-octyl salicylic acid, 5-n-heptyl salicylic acid, 4-n-heptyl salicylic acid and 2-hydroxy-3-methyl benzoic acid or its alkoxy derivatives, such as 2-hydroxy-3-methoxy benzoic acid. Exemplary retinoids include, but are not limited to, retinoic acid (e.g., all-trans or 13-cis) and its derivatives, retinol (vitamin A) and its esters, such as retinyl palmitate, retinyl acetate and retinyl propionate and its salts. Preferred exfoliating promoters are 3,6,9-trioxa undecane dioic acid, glycolic acid, lactic acid or any mixture thereof. Other acids, such as oxa acids (e.g., U.S. Pat. No. 6,069,169) and oxa dioic acids (e.g., U.S. Pat. No. 5,932,229) may be included in the compositions of the present disclosure.

[0079] When the present disclosure includes an exfoliation accelerator, the composition generally includes about 0.5 wt % to 30 wt %, preferably about 1 wt % to about 15 wt %, more preferably about 2 wt % to about 10 wt %, and most preferably about 4 wt % of the exfoliation accelerator based on the total weight of the composition.

[0080] Antioxidants particularly act to scavenge free radicals from the skin to protect the skin from environmental damage. Examples of antioxidants that can be used in the compositions of the present invention include compounds with phenolic hydroxyl functions, such as ascorbic acid and its derivatives / esters; beta-carotene; catechins; curcumin; ferulic acid derivatives (e.g., ethyl ferulate or sodium ferulate); gallic acid derivatives (e.g., propyl gallate); lycopene; reducing acids; rosmarinic acid; tannic acid; tetrahydrocurcumin; tocopherol and its derivatives; uric acid; or any mixture thereof. Other suitable antioxidants are antioxidants with one or more thiol (-SH) functions, in reduced or non-reduced form, such as glutathione, lipoic acid, thioglycolic acid and other sulfhydryl compounds. Antioxidants can be inorganic, such as bisulfites, metabisulfites, sulfites or other inorganic salts and acids containing sulfur. The compositions of the present disclosure may include an antioxidant, preferably in an amount of about 0.001 wt % to about 10 wt %, and more preferably in an amount of about 0.001 wt % to about 5 wt %, based on the total weight of the composition.

[0081] Composition of the present disclosure can also include one or more of the following ingredients, additives or adjuvants: anesthesia, antiallergic, antifungal, antibacterial, chelating agent, coloring agent, dye, mitigation agent, emollient, emulsifier, fragrance, filler, wetting agent, lubricant, moisturizer, pH adjusting agent, pigment modifier, preservative, skin penetration enhancer, stabilizer, surfactant, thickening agent, viscosity modifier, hydrophilic or lipophilic gelling agent, vitamin or any mixture thereof. The amount of these various ingredients, additives, adjuvants or activating agents is the amount routinely used in cosmetics or pharmaceutical fields, and for example they can account for about 0.01% to 20% of the gross weight of the composition. In addition, adjuvant, ingredient, additive or activating agent can be introduced into the fat phase, the liquid phase and / or the lipid vesicle according to its property.

[0082] The components of the present disclosure are preferably included in a cosmetically acceptable medium (i.e., vehicle, diluent or carrier). In terms of covering topical application, the compositions of the present disclosure are media compatible with human skin. The compositions can be formulated as aqueous, alcohol or aqueous / alcohol-based solutions, ointments, lotions, gels, water-in-oil, oil-in-water or water-oil-water triple emulsions, which have the outward appearance of an emulsion or gel, microemulsion or aerosol. In addition, as described above, the compositions can be in the form of vesicle dispersions containing ions and / or nonionic lipids. The dosage unit suitable for such pharmaceutical compositions is formulated according to the conventional knowledge and technology used in the art.

[0083] More specifically, the composition for topical application may be in the form of a protective care composition for the skin, preferably for the face, neck, hands, feet or other areas of the body. Non-limiting examples include day creams or lotions, night creams or lotions, sunscreens, lotions or oils, body milks, makeup (foundations), artificial tanning compositions and depilatories.

[0084] If the composition of the present disclosure is an emulsion, then relative to the gross weight of the composition, the ratio of the fatty substance contained therein can be in the range of about 5 wt % to 80 wt %, preferably about 5 wt % to 50 wt %. The fatty substance and emulsifier used in the composition of the emulsion form are one or more of those conventionally adopted in cosmetics or pharmaceutical fields. The non-limiting examples of fatty substances include mineral oil (for example, petroleum jelly), vegetable oil and hydrogenated derivatives thereof, animal oil, synthetic oil (for example, perhydrosqualene), silicone oil (for example, polydimethylsiloxane) and fluoro oil. Other exemplary fatty substances include fatty alcohol (for example, stearyl alcohol or cetyl alcohol), fatty acid (for example, stearic acid) and wax.

[0085] The emulsifier is generally present in the composition of the present disclosure in an amount of about 0.3 wt % to 30 wt %, and preferably about 0.5 wt % to 30 wt %, relative to the total weight of the composition.

[0086] In one aspect, the present disclosure relates to administering an effective amount of at least one flower extract of one or more species of lotus composition or a composition comprising the same to inhibit or block the release of acetylcholine at NMJ through nerve cells, thereby relaxing and / or decontracting subcutaneous muscles, especially facial expression muscles, thereby smoothing the skin or eliminating skin wrinkles, wherein the inhibition of acetylcholine release at NMJ through nerve cells corresponds to relaxation or decontraction of muscles, preferably facial expression muscles.

[0087] In yet another aspect, the present disclosure encompasses a method of treating hyperhidrosis or perspiration, the method comprising applying to the skin a composition containing a flower extract of one or more species of Lotus in a cosmetically and / or dermatologically acceptable medium, and in an amount effective to treat, reduce, prevent and / or ameliorate hyperhidrosis or perspiration. The application of the composition containing a flower extract of one or more species of Lotus is preferably topical.

[0088] Another aspect of the present disclosure relates to a method of improving the aesthetic appearance of the skin and comprises applying to the skin or introducing by a targeted delivery mode a composition of one or more flower extracts of one or more species of the lotus composition in an amount effective to improve the aesthetic appearance of the skin. According to this aspect, the aesthetic appearance relates to treating at least one condition, such as signs of dermatological aging. Dermatological aging can include chronological aging, actinic aging, hormonal aging, or any combination thereof.

[0089] As will be appreciated by practitioners, cosmetic treatments comprising compositions containing flower extracts of one or more species of the lotus composition of the present disclosure can be carried out, for example, by topical application of a cosmetic composition as described herein, according to conventional techniques for applying such compositions. Conventional and commonly used techniques encompass application of creams, lotions, gels, serums, ointments, antiperspirants or deodorants to the skin; spraying as a form of application is also contemplated.

[0090] In addition to the active ingredients, the disclosed cosmetic compositions may contain any number of additional ingredients, such as, but not limited to: additional active ingredients (e.g., cosmetic, dermatological and / or pharmaceutical), alcohols, allergy suppressants, amino acids, anti-acne agents (e.g., salicylic acid), anti-aging agents, antibacterial agents, antifungal agents, analgesics, anti-hair loss agents, anti-wrinkle agents, antibacterial agents, antimicrobial agents, antioxidants, anti-inflammatory agents, burn healing agents, colorants (e.g., lakes, pigments), depigmenting agents, deodorants, dyes, emollients (e.g., glycerin, butylene glycol), excipients, fatty substances, fillers, film formers (e.g., dimethicone copolymer, ethylhexyl acrylate copolymer), fragrances , free radical scavengers, glycerin, glyceryl monostearate, glyceryl distearate, hair growth agents, conditioners, hair softeners, hair moisturizers, herbal extracts, humectants (e.g., hyaluronic acid, orotic acid, lipoproteins), insect repellents, drugs, moisturizers, inactive carrier oils (e.g., triglycerides, silicone oils, mineral oils), oils, peptides, polypeptides, proteins, fragrances, pigments, preservatives, plasticizers, reflective agents, sebum absorbers, skin lighteners, sunscreens, surfactants, tanning agents, thickeners (hydroxyethylcellulose, xanthan gum, carbomer), petrolatum, vasoconstrictors, vasodilators, vitamins (e.g., vitamin A, vitamin E), water, waxes and / or combinations thereof.

[0091] In addition to the active ingredients, the disclosed cosmetic compositions may contain any number of additional ingredients, such as, but not limited to: additional active ingredients (e.g., cosmetic, dermatological and / or pharmaceutical), alcohols, allergy suppressants, amino acids, anti-acne agents (e.g., salicylic acid), anti-aging agents, antibacterial agents, antifungal agents, analgesics, anti-hair loss agents, anti-wrinkle agents, antibacterial agents, antimicrobial agents, antioxidants, anti-inflammatory agents, burn healing agents, colorants (e.g., lakes, pigments), depigmenting agents, deodorants, dyes, emollients (e.g., glycerin, butylene glycol), excipients, fatty substances, fillers, film formers (e.g., dimethicone copolymer, ethylhexyl acrylate copolymer), fragrances , free radical scavengers, glycerin, glyceryl monostearate, glyceryl distearate, hair growth agents, conditioners, hair softeners, hair moisturizers, herbal extracts, humectants (e.g., hyaluronic acid, orotic acid, lipoproteins), insect repellents, drugs, moisturizers, inactive carrier oils (e.g., triglycerides, silicone oils, mineral oils), oils, peptides, polypeptides, proteins, fragrances, pigments, preservatives, plasticizers, reflective agents, sebum absorbers, skin lighteners, sunscreens, surfactants, tanning agents, thickeners (hydroxyethylcellulose, xanthan gum, carbomer), petrolatum, vasoconstrictors, vasodilators, vitamins (e.g., vitamin A, vitamin E), water, waxes and / or combinations thereof.

[0092] Sunscreen components can include zinc oxide, titanium dioxide, octinoxate, ensulizole, ethylhexyl triazone, octylsalicylate, butyl methoxydibenzoylmethane, bis-ethylhexyloxyphenol methoxyphenyl triazine, isoamyl p-methoxycinnamate, diethylhexyl butyramido triazone or a mixture thereof. In some aspects, the sunscreen component may not contain octocrylene. In addition, according to some aspects, the sunscreen formulations described herein are eco-compatible compositions. For example, the sunscreen formulations are compatible with biological life and are harmless to marine ecosystems (particularly for tropical environments with coral reefs). Specifically, the sunscreen formulations do not contain components that inhibit cell growth or cell replication in marine environments. In some examples, this can be achieved by the following sunscreen composition: free of bis-ethylhexyloxyphenol methoxyphenyl triazine (BEMT), cinnamate, benzophenone, camphor derivatives, titanium oxide, tea tree essential oil, ylang-ylang flower extract, Bambousa Arundinacea extract, shea butter (Karitè), animal-derived fats, ali root oil, tocopheryl acetate, tocopherol, retinol, polyphenolic compounds extracted from green tea, or mixtures thereof.

[0093] The compositions of the present disclosure may further include other cosmetic ingredients, such as, but not limited to, humectants, emollients, moisturizers, anti-wrinkle ingredients, concealers, matte polishes, pigments, colorants, proteins, antioxidants, contour powders, chelating agents, emulsifiers, ultraviolet (UV) absorbers, oil absorbers, anti-foaming agents, anti-tack agents, thickeners, fragrances, preservatives, antimicrobial agents, antifungal agents, neutralizers, vitamins, plasticizers, cohesive agents, alkalizing and acidifying agents, fillers, solvents, and mixtures thereof.

[0094] The composition may contain additional ingredients such as alkalizing agents, emulsifiers, emollients, plasticizers, preservatives, humectants, moisturizers, solvents and tonicity agents or active ingredients suitable for providing anti-aging benefits. Examples of preferred additional ingredients include glycerol.

[0095] Additional ingredients may optionally be added to the disclosed compositions, as described in detail below.

[0096] Colorants or Pigments: The composition may include one or more cosmetic powders, such as calcium aluminum borosilicate, PMMA, polyethylene, polystyrene, methyl methacrylate crosspolymer, nylon-12, ethylene / acrylic acid copolymer, boron nitride, Teflon, silica, and the like. Typically, the composition will include a colorant or pigment to impart a desired color or effect, examples being inorganic pigments, organic pigments, and / or lakes. Exemplary inorganic pigments include, but are not limited to, metal oxides and metal hydroxides, such as magnesium oxide, magnesium hydroxide, calcium oxide, calcium hydroxide, aluminum oxide, aluminum hydroxide, iron oxide (ot-Fe 2 O 3 ,γ-Fe 2 O 3 , Fe 3 O 4 , FeO), red iron oxide, yellow iron oxide, black iron oxide, iron hydroxide, titanium dioxide, titanium lower oxide, zirconium oxide, chromium oxide, chromium hydroxide, manganese oxide, cobalt oxide, cerium oxide, nickel oxide and zinc oxide and composite oxides and composite hydroxides such as iron titanate, cobalt titanate and cobalt aluminate. Non-metallic oxides such as aluminum oxide and silicon dioxide, ultramarine blue (i.e., sodium aluminum silicate containing sulfur), Prussian blue, manganese violet, bismuth oxychloride, talc, mica, sericite, magnesium carbonate, calcium carbonate, magnesium silicate, magnesium aluminum silicate, silicon dioxide, titanated mica, iron oxide titanated mica, bismuth oxychloride, etc. are also considered as suitable inorganic pigments. Organic pigments may include, but are not limited to, at least one of carbon black, carmine, phthalocyanine blue and green pigments, diarylide yellow and orange pigments and azo red and yellow pigments such as toluidine red, lithol red, naphthol red and brown pigments and combinations thereof.

[0097] Lakes generally refer to colorants prepared from water-soluble organic dyes (e.g., D&C or FD&C) that have been precipitated onto an insoluble reactive or adsorptive matrix or diluent. As used herein, the term "D&C" means drug and cosmetic colorants approved by the FDA for use in drugs and cosmetics. As used herein, the term "FD&C" means food, drug and cosmetic colorants approved by the FDA for use in foods, drugs and cosmetics. Certified D&C and FD&C colorants are listed at 21 CFR §74.101 et seq. and include FD&C colors Blue 1, Blue 2, Green 3, Orange B, Tangerine 2, Red 3, Red 4, Red 40, Yellow 5, Yellow 6, Blue 1, Blue 2, Orange B, Tangerine 2 and D&C colors Blue 4, Blue 9, Green 5, Green 6, Green 8, Orange 4, Orange 5, Orange 10, Orange 11, Red 6, Red 7, Red 17, Red 21, Red 22, Red 27, Red 28, Red 30, Red 31, Red 33, Red 34, Red 36, Red 39, Violet 2, Yellow 7, Yellow 8, Yellow 10, Yellow 11, Blue 4, Blue 6, Green 5, Green 6, Green 8, Orange 4, Orange 5, Orange 10, Orange 11 et seq. Suitable substrates for forming the lake include, but are not limited to, mica, bismuth oxychloride, sericite, alumina, aluminum, copper, bronze, silver, calcium, zirconium, barium and strontium, titanated mica, filmed silica, spherical silica, polymethyl methacrylate (PMMA), micronized Teflon, boron nitride, acrylate copolymers, aluminum silicate, aluminum starch octenylsuccinate, bentonite, calcium silicate, cellulose, chalk, corn starch, diatomaceous earth, bleaching earth, glycerol, Oil starch, hectorite, hydrated silica, kaolin, magnesium aluminum silicate, magnesium trisilicate, maltodextrin, montmorillonite, microcrystalline cellulose, rice starch, silicon dioxide, talc, mica, titanium dioxide, zinc laurate, zinc myristate, zinc rosinate, alumina, ahapulgite, calcium carbonate, calcium silicate, dextran, nylon, silica silylate, silk powder, sericite, soy flour, tin oxide, titanium hydroxide, trimagnesium phosphate, walnut shell powder and mixtures thereof. Suitable lakes include, but are not limited to, lakes of red dyes from the monoazo, disazo, Norm, xanthene, or indigo families, such as Red 4, 6, 7, 17, 21, 22, 27, 28, 30, 31, 33, 34, 36, and Red 40; lakes of yellow pyrazole, monoazo, fluorescein, xanthene, quinoline, dyes or their salts, such as Yellow 5, 6, 7, 8, 10, and 11; lakes of violet dyes, including violet dyes from the anthraquinone family, such as Violet 2, and lakes of orange dyes, including Orange 4, 5, 10, 11, etc. Suitable lakes for D&C and FD&C dyes are defined in 21 CFR §82.51.

[0098] Coloring agent can be optionally surface treated, for example, to make the particle more hydrophobic or more dispersible in the medium. The surface of the particle can be, for example, covalently or ionically bonded to an organic molecule or a silicon-based molecule, or can be adsorbed to an organic molecule or a silicon-based molecule, or the particle can be physically coated with a material layer. The surface treatment compound can be attached to the particle by any suitable coupling agent, linker group or functional group (such as silane, ester, ether, etc.). The compound can include a hydrophobic portion, which can be selected from, for example, alkyl, aryl, allyl, vinyl, alkyl-aryl, aryl-alkyl, organosilicone, diorganosilicone, dimethicone, methylsilicone, polyurethane, silicone-polyurethane and its fluorine derivatives or perfluorinated derivatives. Other hydrophobic modifiers include lauroyl lysine, isopropyl titanium triisostearate (ITT), ITT and dimethicone (ITT / dimethicone) crosspolymer, ITT and amino acids, ITT / triethoxycaprylylsilane crosspolymer, waxes (e.g., carnauba), fatty acids (e.g., stearate), HDI / trimethylol caprolactone crosspolymer, PEG-8 methyl. For example, triethoxysilane, aloe, jojoba esters, lecithin, perfluoroalcohol phosphates, and magnesium myristate (MM).

[0099] The optional pigment component includes a colorant surface treated with an alkylsilane, the colorant consisting essentially of or comprising an alumina substrate (e.g., platelet-shaped) and a pigment, dye, or lake bonded to the alumina substrate by an alkylsilane surface treatment. Typically, the alkylsilane will be a octylsilane and can be formed by treatment with triethoxyoctanoylsilane. Non-limiting examples of such colorants include, but are not limited to, alumina / titanium dioxide / triethoxycaprylyl silane 1% (COVALUMINE Atlas White AS), alumina / D&C Red Aluminum Lake CTD / triethoxycaprylyl silane 1% (COVALUMINE Red Rose AS), alumina / D&C Red Aluminum Lake CTD / triethoxycaprylyl silane 1% (COVALUMINE Sonoma Red AS), alumina / black iron oxide CTD / triethoxycaprylyl silane 1% (COVALUMINE Sonoma Black AS), alumina / D&C Red #6 Aluminum Lake CTD / triethoxycaprylyl silane 1% (COVALUMINE Fire Red AS), alumina / yellow 1 iron oxide CTD / triethoxycaprylyl silane 1% (COVALUMINE Sonoma Yellow AS), alumina / D&C Blue #1 Aluminum Lake CTD / triethoxycaprylyl silane 1% (COVALUMINE Astral Blue AS), alumina / carmine red CTD / triethoxycaprylyl silane 1% (COVALUMINECampari AS), alumina / yellow #5 CTD / triethoxycaprylyl silane 1% (COVALUMINE Sunburst AS), alumina / triethoxycaprylyl silane 1% and combinations thereof, each of which is available from SENSIENT Cosmetic Technologies LCW.

[0100] Interference or pearlescent pigments may also be included. These typically contain TiO 2 , Fe 2 O 3 or Cr 2 O 3Micas layered with films of about 50 to 300 nm, such as those of titanium oxide or bismuth oxychloride, etc. These include white nacreous materials such as mica covered with titanium oxide or covered with bismuth oxychloride; and colored nacreous materials such as titanium mica covered with iron oxide, titanium mica covered with iron blue or chromium oxide, titanium mica covered with organic pigments of the aforementioned types. If these materials are used, it is preferred that these materials are used together in an amount of less than 1.0 wt%. Preferably, the pearlescent component has a pearlescent component based on bismuth oxychloride or reflective pearls. Bismuth oxychloride matches the natural pearlescence of the skin better than compounds such as titanium oxide that provide a more artificial appearance. Bismuth oxychloride better mimics the natural reflection of the skin. However, other pearlescent components may be used. The preferred pearlescent component is called CHROMA-LITE, which is a combination of colored pigments bonded to BI-LITE 20 (bismuth oxychloride and mica) using calcium stearate. CHROMA-LITE components are available from Englehard Corporation (Iselin, NJ) in various shades / colors.

[0101] Cosmetically acceptable vehicle: The disclosed compositions will typically include a cosmetically acceptable vehicle. As used herein, "cosmetically acceptable" means that it is safe when in contact with the human integument. The vehicle may include a liquid, including a single phase, a two-phase system, or an emulsion. Emulsions include oil-in-water, silicone-in-water, water-in-oil, water-in-silicone, and the like. When formulated into an emulsion, an emulsifier is typically included. It is specifically mentioned that it can be made from volatile silicones (e.g., cyclopentasiloxane), hydrocarbons, ester oils, lower alcohols (e.g., ethanol, isopropanol, etc.) and water.

[0102] Emollients: Emollients may be used in the present disclosure and include, but are not limited to, ester oils, which will typically be in the form R 4 (COOH) 1-2 The acid and form R 5 (OH) 1-3 The etherification product of alcohol, wherein R 4 and R 5 Each is independently a linear, branched or cyclic hydrocarbon group, optionally containing unsaturated bonds, and having 1 to 30 carbon atoms, preferably 2 to 30 carbon atoms, and more preferably 3 to 30 carbon atoms, optionally substituted with one or more functional groups including hydroxyl, oxa, oxo, etc. Preferably, R 4 and R 5 At least one of the emollients comprises at least one fatty chain. The esters defined above will include, but are not limited to, esters of monoacids with monools, monoacids with diols and triols, diacids with monools, and triacids with monools. Other emollients include polydimethicone. Wetting agents such as glycerol and other C1-10 polyols or diols may also be included.

[0103] Adsorbent powder: Adsorbent powders that absorb oil to improve comfort may be added. Examples of suitable adsorbent powders may include silica, mica, elastomers, kaolin, starch, or mixtures thereof.

[0104] Fillers: Fillers may also be optionally added in an amount of about 1 wt % to about 20 wt %, preferably about 1 wt % to about 10 wt % of the final composition. Examples of fillers include, but are not limited to, silica, PMMA, nylon, alumina, barium sulfate, or any other filler commonly used in such compositions.

[0105] Film formers: polymeric film formers include cellulose, polyolefins, polyethylene, polyacrylates, polyurethanes, silicones, silicone acrylates, polyamides, polyesters, fluoropolymers, polyethers, polyacetates, polycarbonates, polyimides, rubbers, epoxy resins, formaldehyde resins, and homopolymers and copolymers of any of the foregoing. In particular, when present, the film former may range from about 2 wt% to about 20 wt% of the active component, may range from about 7 wt% to about 13 wt% of the active component, less than, equal to or greater than about 2 wt%, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 or about 20 wt%. As described herein above, the film former may be used to help produce a uniform mixture of the extract solution and to help evenly distribute the composition on the user's skin. According to various aspects, the film former may include acrylate copolymers, acrylate copolymers (and) acrylate / polytrimethylsiloxymethacrylate copolymers, acrylate / octylacrylamide copolymers, acrylate / C1-2 succinate / hydroxyacrylate copolymers, acrylate / C12-22 alkyl methacrylate copolymers, acrylate / dimethicone copolymers, acrylate / ethylhexyl acrylate copolymers, acrylate / hydroxyacrylate copolymers, acrylate / lauryl acrylate / stearyl acrylate / ethylene oxide methacrylate copolymers, acrylate / octylacrylamide copolymers, acrylate / polytrimethylsiloxymethacrylate copolymers, acrylate / polytrimethylsiloxymethacrylate copolymers (and) laureth-1 phosphate, acrylate / tert-butylacrylamide copolymers, adipic acid / diethylene glycol crosspolymers, adipic acid / neopentyl glycol / trimellitic anhydride copolymers, alginate starch octenylsuccinate aluminum AMP-acrylate copolymers, behenyl methacrylate / methyl methacrylate copolymers T-Butyl Acrylate Copolymer, Rapeseed / Tung Oil Copolymer, Butyl Acrylate / Hydroxypropyl Dimethicone Acrylate Copolymer, Butyl PVM / MA Copolymer, C24-28 Alkyl Dimethylsiloxy Trimethylsiloxysilicate, Caprylyl Glycerin / Sebacic Acid Copolymer, Diisostearyl Polyglyceryl-3 Dimer Dilinoleate, Ethyl PVM / MA Copolymer, Isobutylene / Ethyl Maleimide / Hydroxyethyl Maleimide Copolymer, Isopropyl PVM / MA Copolymer, Ethyl Methacryloyl Betaine / Acrylates Copolymer, Octyl Acrylamide / Acrylates / Butylaminoethyl Methacrylate Copolymer, Phenylpropyl Dimethylsiloxysilicate, Polyamide-3, Polyamide-8, Polyester-10, Polyester-7, Polyethylene, Polymethylsilsesquioxane, Polyurethane-1, Polyurethane-14 (and) AMP-acrylate Copolymer, Polyurethane-18, Polyurethane-34, Polyurethane-35, Polyurethane-48, Polyurethane-64, Polyurethane-93, Polyethylene Stearyl Ether, PPG-17 / IPDI / DMPA Copolymer,PPG-26 / dimer dilinoleate copolymer, pullulan, PVP, PVP crosspolymer, sodium polyacrylate, sodium polystyrene sulfonate, styrene / acrylates copolymer, styrene / VP copolymer, triacontyl PVP, trifluoropropyl dimethylsiloxy / trimethylsiloxy polysilsesquioxane, trimethylpentanediol / adipic acid / glycerin crosspolymer, trimethylsiloxysilicate, VA / butyl, maleate / isobornyl acrylate copolymer, VA / crotonate copolymer, VA / crotonate / vinyl neodecanoate copolymer, vinyl caprolactam / VP / dimethylaminoethyl methacrylate copolymer, VP / hexadecene copolymer, VP / acrylates / lauryl methacrylate copolymer, VP / dimethylaminoethyl methacrylate copolymer, VP / DMAPA acrylates copolymer, VP / eicosene copolymer, VP / hexadecene copolymer, VP / methacrylamide / vinylimidazole copolymer, VP / VA copolymer, VP / vinyl caprolactam / DMAPA acrylates copolymer, copolymers thereof or mixtures thereof. In a specific aspect, the film former may include sodium polyacrylate.

[0106] Oil: Suitable non-limiting examples of oils for the oil phase (e.g., in an emulsion) include natural and synthetic oils, including animal oils, vegetable oils, and petroleum oils; fatty acid triglycerides; fatty acid esters, such as octyl palmitate, isopropyl myristate, and isopropyl palmitate; ethers, such as dicaprylyl ether; fatty alcohols, such as cetyl alcohol, stearyl alcohol, and behenyl alcohol; sterols; hydrocarbons, such as isooctane, isododecane, isohexadecane, decane, dodecane, tetradecane, tridecane, C 8-20 Isoparaffin, mineral oil, petrolatum, isoeicosane and polyisobutylene; C 10-30 Cholesterol / lanosterol esters; lanolin, etc. Representative hydrocarbons include paraffins available from Exxon under the ISOPARS trademark and from Permethyl Corporation. In addition, C 8-20 Paraffins, such as C manufactured by Permethyl Corporation under the trade name PERMETHYL 99ATM. 12 Isoparaffin (isododecane) is suitable. Commercially available C 16 Isoparaffins, such as isohexadecane (with the trade name PERMETHYL.RTM.) are also suitable. Silicone oils such as dimethicone, cyclic silicones and polysiloxanes can also be included in the oil phase. In one aspect, the silicone oil is present in an amount of less than about 5% by weight of the oil phase.

[0107] Solvent: The cosmetically acceptable vehicle may include a volatile solvent. Typically, a volatile solvent may have a vapor pressure greater than about 0.01 mmHg at 20 degrees Celsius. Volatile solvents may include volatile C5-12 Hydrocarbons (e.g., isododecane), aromatic hydrocarbons (e.g., xylene, toluene, etc.), ketone acetone, methyl ethyl ketone, etc.), ethers (e.g., diethyl ether, methyl ethyl ether, etc.), perfluorocarbons, hydrofluoroethers, freons, acetates (e.g., ethyl acetate, butyl acetate, etc.), etc. Preferred volatile solvents will be cosmetically acceptable. The solvent may be an organic solvent. When present, the solvent accounts for about 10 wt % to about 50 wt % of the active ingredient, about 30 wt % to about 40 wt % of the active ingredient, and less than, equal to or greater than about 30 wt %, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49 or about 50 wt % of the composition. Examples of organic solvents may include an isododecane component, an alkyl benzoate component, and a neopentyl glycol diheptanoate component. Isododecane (an example of which is represented by CAS 31807-55-3) is generally understood to be a transparent, colorless and odorless volatile liquid, which makes it suitable for use in makeup such as mascara, eyeliner, lip products or any product that requires improved wear characteristics and does not require residue. It does not leave an oily residue. Isododecane is a volatile lipophilic component for deodorant spray and hair care applications. It is a hydrocarbon component used as a solvent. The alkyl benzoate component can be a C12-15 alkyl benzoate (an example of which is represented by CAS 68411-27-8), which is generally understood to be a low molecular weight ester of benzoic acid and C12-15 alcohol. C12-15 alkyl benzoate is an almost odorless transparent liquid. In some instances, the alkyl benzoate component includes C12 alkyl benzoate, C13 alkyl benzoate, C14 alkyl benzoate, C15 alkyl benzoate or a mixture thereof. Neopentyl glycol diheptanoate (an example of which is represented by CAS 68855-18-5) is generally understood to be a mixture of the texture enhancing ingredient neopentyl glycol and the fatty acid heptanoate of grape origin.

[0108] According to various aspects of the present disclosure, the composition is substantially free of cyclopentasiloxane. For example, the composition may include less than about 10wt% cyclopentasiloxane, less than about 9wt%, less than about 8wt%, less than about 7wt%, less than about 6wt%, less than about 5wt%, less than about 4wt%, less than about 3wt%, less than about 2wt%, less than about 1wt%, less than about 0.5wt%, less than about 0.1wt%, less than about 0.01wt% or less than about 0.001wt%. In some aspects, the composition is completely free of (e.g., including 0wt%) cyclopentasiloxane. The disclosed isododecane component, alkyl benzoate component and neopentyl glycol diheptanoate component together play an effective role in replacing cyclopentasiloxane while having similar, equal or excellent performance. It may be desirable to replace cyclopentasiloxane because despite its performance in the composition, the regulation scheme seeks to limit the use of cyclopentasiloxane in cosmetic products. Therefore, it is important to find an effective substitute for cyclopentasiloxane. Suitable substitutes may include oleochemical components such as dicaprylyl carbonate, dicaprylyl ether or mixtures thereof.

[0109] The surface of all or part of the individual pigments of the pigment component can be at least partially coated. Examples of suitable coatings include lauroyl lysine coatings, silane coatings, methicone coatings, dimethicone coatings, stearoyl glutamic acid or combinations thereof. As is generally understood, lauroyl lysine is an amino acid derivative derived from the natural product lysine, i.e., protein and lauric acid, another natural coconut fatty acid. The lauroyl lysine treatment produces a hydrophobic system with enhanced dispersibility, increased abrasion properties and a moist feel for the cosmetic on the skin. Compared with the silane treatment, the pigment surface is smooth, flat and has less fluff, so it is easier to press. The lauroyl lysine coating is lubricating, thereby giving the substrate a smooth, moist feel. As is generally understood, the silane coating is the deposition of a coating achieved by the reaction of silanols with the pigment. By hydrolyzing the "Si-OR" group to silanols (Si-OH), alkyl silanes can self-associate or hydrogen bond to the pigment surface, so that a stable bond is formed between the pigment and the treatment compound after curing. The silicone-treated pigment is well dispersed in cyclomethicone. The surface tension of silicone is very low, so it has excellent hydrophobicity and better lipophilicity because it is easily dispersed in mineral oil, ester and silicone fluid. The pigment treated with alkyl silane is more hydrophobic than the pigment treated with methicone, is more wet in commonly used cosmetic oils and has a lower oil absorption rate. In the hydrated dense formulation, the excellent wettability imparted by the alkyl silane treatment allows the incorporation of high pigment loads to achieve a "powdery" feel when applied to the skin while maintaining a low melt viscosity for hot filling. As generally understood, the methicone coating is a polymethylhydrogensiloxane, which absorbs trace amounts of water from the pigment surface and converts Si-H bonds to Si-OH under the condition of releasing a small amount of hydrogen, and forms a covalent Si-O pigment chemical bond when solidified. The coating formed is highly hydrophobic and is not dissolved by ordinary solvents. The pigment is fully wetted in oil, specifically in silicone oil. The skin feels a little dry, with enhanced slip and diffusibility. As is generally understood, a dimethicone coating (also known as polymethylsiloxane) is a coating that is bonded to the pigment or filler surface by a mechanism of hydrolysis, condensation, and curing to Si-O pigment bonds. Surfaces treated with dimethicone are quite hydrophobic and have good slip and a more lubricated feel. It can be used in oil-based systems that can be used in water-free products.

[0110] With respect to individual pigments, the coating can substantially coat 100% of the total surface area of ​​the individual pigments. In some additional aspects, the coating can coat about 5% to about 95% of the total surface area of ​​the individual pigments, about 10% of the total surface area to about 90% of the total surface area, about 15% of the total surface area to about 10% of the total surface area, about 20% of the total surface area to about 80% of the total surface area, about 25% of the total surface area to about 75% of the total surface area, about 30% of the total surface area to about 70% of the total surface area, about 35% of the total surface area to about 10% of the total surface area, about 1 ... about 65% of the total surface area, about 40% to about 60% of the total surface area, about 45% to about 50% of the total surface area, less than, equal to, or greater than about 5% of the total surface area, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24% of the total surface area , 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60 %, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, or about 95%. In some aspects, at least a portion of the total number of pigments present can be substantially free of any coating.For example, when less than 100% of the total number of pigments comprises a coating, about 5% to about 95% of the total number of pigments may comprise a coating, about 10% to about 90% of the total number of pigments may comprise a coating, about 15% to about % of the total number of pigments may comprise a coating, about 20% to about 80% of the total number of pigments may comprise a coating, about 25% to about 75% of the total number of pigments may comprise a coating, about 30% to about 70% of the total number of pigments may comprise a coating, about 35% to about 65% of the total number of pigments may comprise a coating, about 40% to about 60% of the total number of pigments may comprise a coating, about 45% to about 50% of the total number of pigments may comprise a coating, less than, equal to, or greater than about 5% of the total number of pigments may comprise a coating, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59 %. The pigment may be present in an amount of about 60%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94% or about 95%. Each pigment may be coated with the same material, or different parts of the pigment may be at least partially coated with different coatings. An example of a suitable coating is stearoyl glutamic acid.

[0111] In some examples where the coating is stearoyl glutamate, the composition includes triglycerol diisostearate, glyceryl stearate, or a combination thereof.

[0112] Thickeners: Suspending agents and thickeners generally include silica gels, gums, clays, fumed silica, fatty acid soaps, and various hydrocarbon gels, and other ingredients that remain on the surface of keratinous tissue when incorporated into the formulation, as disclosed in the International Cosmetic Dictionary and Handbook (12th Edition), which is hereby incorporated by reference. Thickeners, such as gelling agents, may also be used. Examples include bentone, triglycerides, aluminum stearate, C18-C36 acid glycol esters, tribehenin, glyceryl monostearate, alginates, carbomers, cellulose, gums, carrageenan, starch, or silicates.

[0113] Waxes: Waxes that may be used in the present disclosure include, but are not limited to, linear polyethylene, microcrystalline petroleum wax, carnauba wax, montan wax, carnauba wax, rice bran wax, castor wax, ash wax, stearone, synthetic fatty acid esters, bayberry wax, castor wax, Japan wax, ozokerite, beeswax, candelilla wax, petrolatum, microcrystalline wax, cocoa butter, terminalia butter, thatch wax, shellac wax, glycol diesters or triesters of C18-C36 fatty acids, spermaceti, paraffin, hard animal tallow oil, lanolin, lanolin alcohol, cetyl alcohol, glyceryl monostearate, sugarcane wax, jojoba wax, stearyl alcohol, silicone wax, and combinations thereof.

[0114] In some aspects of the present disclosure, the composition may include a preservative. Where present, the preservative may range from about 0.0001 wt % to about 0.5 wt %, from about 0.0003 wt % to about 0.1 wt %, less than, equal to, or greater than about 0.0001 wt %, 0.0003, 0.0005, 0.001, 0.005, 0.01, 0.05, 0.1, or about 0.5 of the composition.

[0115] When included, the preservative is included in an amount sufficient to prevent spoilage or prevent the growth of inadvertently added microorganisms for a specific period of time, but insufficient to contribute to the odor neutralization properties of the composition. In other words, the preservative does not act as an antimicrobial compound for killing microorganisms on the surface on which the composition is deposited to eliminate odors produced by microorganisms. Instead, it is used to prevent spoilage of the composition to increase shelf life.

[0116] The preservative can be any organic preservative material that does not cause damage to the appearance of the fabric, such as discoloration, staining, bleaching. Suitable water-soluble preservatives include organic sulfur compounds, halogenated compounds, cyclic organic nitrogen compounds, low molecular weight aldehydes, parabens, propylene glycol materials, isothiazolinones, quaternary compounds, benzoates, low molecular weight alcohols, dehydroacetic acid, phenyl and phenoxy compounds or mixtures thereof.

[0117] Non-limiting examples of commercially available water-soluble preservatives include a mixture of about 77% 5-chloro-2-methyl-4-isothiazolin-3-one and about 23% 2-methyl-4-isothiazolin-3-one; a broad spectrum preservative available under the trade name of Rohm and Haas Co. CG was obtained as a 1.5% aqueous solution; 5-bromo-5-nitro-1,3-dioxane, which is available from Henkel under the trade name Bronidox Obtain; 2-bromo-2-nitropropane-1,3-diol, which is available from Inolex Corporation (Inolex) under the trade name 1,1′-hexamethylenebis(5-(p-chlorophenyl)biguanide) (commonly known as chlorhexidine) and its salts, for example, with acetic acid and diglucuronic acid; a 95:5 mixture of 1,3-bis(hydroxymethyl)-5,5-dimethyl-2,4-imidazolidinedione and 3-butyl-2-iodopropionylcarbamate, available from Lonza under the trade name Glydant N-[1,3-bis(hydroxymethyl)2,5-dioxo-4-imidazolidinyl]-N,N'-bis(hydroxy-methyl)urea, commonly known as diazolinyl urea, is available from Sutton Laboratories, Inc. under the trade name II; N,N″-methylenebis{N′-[1-(hydroxymethyl)-2,5-dioxo-4-imidazolidinyl]urea}, which is commonly known as imidazolidinyl urea, is available from 3V-Sigma under the trade name Unicide from Induchem from Sutton Laboratories, Inc. under the trade name Germall Obtain; polymethoxy bicyclic oxazolidine, which can be obtained from Hüls America under the trade name C; formaldehyde; glutaraldehyde; polyaminopropyl biguanide, available from ICI Americas under the trade name Cosmocil or from Brooks, Inc. under the trade name dehydroacetic acid; and benzothiazolinone, available from Rohm and Haas Company under the trade name Koralone TM B-119 acquired.

[0118] The composition can further include inactive components. The inactive components can account for a range of about 50 wt % to about 90 wt % of the composition, a range of about 75 wt % to about 85 wt % of the composition, less than, equal to or greater than about 50 wt %, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89 or about 90 wt % of the composition. When an inactive ingredient is present, the active ingredient comprises from about 10 wt % to about 50 wt % of the composition, comprises from about 15 wt % to about 25 wt % of the composition, less than, equal to, or greater than about 10 wt %, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or about 50 wt % of the composition.

[0119] On the one hand, the composition is a pretreatment composition, which is composed of a non-colored formulation containing an active component, for application to the epidermis to reduce sebum before applying a colored cosmetic composition. Any part of the above-mentioned composition can be combined in any desired manner to produce many different types of cosmetic compositions. The cosmetic composition can be applied to the epithelial layer of the user's skin. For example, the cosmetic composition can be a pretreatment formulation, a makeup formulation, a foundation, a sunscreen, or any other cosmetic applied to the user's epithelial tissue. In addition, any part of the aforementioned composition can be combined to produce a cosmetic formulation that can be applied to the user's keratinous tissue. For example, the cosmetic formulation can be a mascara, a hair dye, a nail polish, a lip gloss, a lipstick or other coloring / colored cosmetic formulation intended to be a long-lasting composition.

[0120] Examples

[0121] The following examples are provided as prophetic examples of potential use and result from testing of formulations using sebum / sweat reducing agents, such as NELUPURE (INCI: Propylene Glycol (and) Glycerin (and) Egyptian Blue Water Lily Extract (and) Chinese Lotus Extract, compared to a control formulation not using such a sebum / sweat reducing agent. Various aspects of the present disclosure may be better understood by reference to the following examples, which are provided as illustrations. The present disclosure is not limited to the examples given herein.

[0122] Example 1: Consumer Comparison of Long-Wear Foundation Compositions

[0123] In a consumer testing survey, consumers will be asked to compare a foundation composition containing a NELUPURE (INCI: Propylene Glycol (and) Glycerin (and) Egyptian Blue Nymphaea Extract (and) Chinese Lotus Extract) extract solution with a commercially available transfer-resistant foundation composition (standard) that does not contain a NELUPURE (INCI: Propylene Glycol (and) Glycerin (and) Egyptian Blue Nymphaea Extract (and) Chinese Lotus Extract) extract solution.

[0124] In a double-blind test, each of the consumer testers applied a layer of each foundation composition to a different half of the cleansed (i.e., not wearing any facial product) face of the consumer tester (e.g., left versus right). The consumers will be asked to compare the feel, comfort, appearance / coverage, and ease of removal of each at different times: after initial application, at 8 hours, and up to 9.9 hours after application, and to answer questions in a questionnaire comparing the two foundation compositions. The data will be tabulated using the Compusense 5 program and analyzed using standard statistical processing procedures.

[0125] It is expected that comments from the above consumer tests indicate that, overall, the foundation composition comprising NELUPURE (INCI: Propylene Glycol (and) Glycerin (and) Egyptian Blue Water Lily Extract (and) Chinese Lotus Extract) extract solution will provide a significantly more natural appearance after 8 hours of wear compared to other standard foundations. It is expected that consumers find that the feel of the new foundation is significantly lighter and more comfortable 8 hours after application when compared to the standard. It is expected that consumers find the disclosed foundation to be breathable and comfortable. As for appearance or coverage, it is expected that consumers agree that the disclosed foundation evens out skin tone, reduces the appearance of fine lines and wrinkles, reduces redness, and reduces the appearance of age spots or sun spots. Overall, it is expected that the disclosed foundation provides a natural and flawless appearance.

[0126] The technical evaluators who oversaw the consumer testing are expected to determine that the new foundation is more visible several hours after application and provides more coverage than the standard foundation. On average, the new foundation is expected to last at least 8 hours. The technical evaluators are expected to deem the new foundation composition "long-wearing".

[0127] Example 2: Consumer Testing of Long-Lasting Foundation Compositions

[0128] In a consumer testing survey, consumer testers will be asked to evaluate the foundation composition of the present invention comprising a solution of NELUPURE (INCI: Propylene Glycol (and) Glycerin (and) Egyptian Blue Water Lily Extract (and) Chinese Lotus Extract) extract. Each consumer tester will apply a layer of the foundation composition to their clean face (i.e., not wearing any facial products). Consumers will be asked to evaluate the feel, comfort, application, appearance, and coverage at different times: upon initial application and 8 hours after application, and answer questions. The questionnaire will contain a series of questions about the aesthetics and performance of the foundation based on a seven-point attribute scale. The expert assessors will also rate the appearance and wear characteristics at the initial time point and after 8 hours of wear compared to the standard.

[0129] In general, the following generally described results are expected: Consumers will like the composition both initially and after 8 hours of wear. The consumer will find that the foundation is easy to apply and blend, providing even coverage and a smooth feel when applied. The consumer will find that the composition is lightweight initially; evens out skin tone; reduces: the appearance of fine lines and wrinkles, the appearance of redness, dark circles under the eyes, age spots / sun spots, and shine; improves the appearance of skin texture; and provides a flawless and natural look. The foundation is expected to be breathable and comfortable, even after 8 hours of wear. The finish, coverage, and color are expected to be maintained after 8 hours of wear. The foundation composition is expected to still control shine and oil invasion while looking freshly applied. After 8 hours, the consumer is expected to find that the foundation has not settled into fine lines, wrinkles, or pores, nor does it look caked or mask-like. Even though the foundation is not expected to transfer after 8 hours, it is expected that most consumers will find the foundation composition easy to remove.

[0130] Example 3 : Inhibits acetylcholine release at the neuromuscular junction

[0131] The aim of the study is to evaluate the effect of 0.2% Nelupure (INCI: Propylene glycol (and) glycerol (and) Egyptian blue water lily extract (and) Chinese lotus extract) on the inhibition of acetylcholine release. The study will be performed using a specific neuromuscular co-culture model. In this model, human fibroblasts will be reprogrammed into pluripotent cells and then differentiated into motor neurons. These motor neurons will be co-cultured with human muscle cells to generate functional neuro-muscular junctions. After incubation with 0.2% Nelupure (INCI: Propylene glycol (and) glycerol (and) Egyptian blue water lily extract (and) Chinese lotus extract), the culture will be activated by glutamate to induce the release of acetylcholine from the motor neurons. After activation, acetylcholine release will be quantified in the cell culture medium by an appropriate ELISA test. Under these experimental conditions, it is expected to be observed that 0.2% Nelupure (INCI: Propylene glycol (and) glycerol (and) Egyptian blue water lily extract (and) Chinese lotus extract) highly significantly inhibits (p<0.0001) acetylcholine release.

[0132] The terms and expressions that have been employed are used as terms of description rather than limitation, and in the use of such terms and expressions, there is no intention to exclude any equivalents of the features shown and described or portions thereof, but it is recognized that various modifications are possible within the scope of the aspects of the present disclosure. Therefore, it should be understood that although the present disclosure is specifically disclosed through specific features, modifications and variations of the concepts disclosed herein may be adopted by those of ordinary skill in the art, and such modifications and variations are considered to be within the scope of the embodiments of the present invention.

[0133] Exemplary aspects.

[0134] The following exemplary aspects are provided, the numbering of which should not be construed as designating a level of importance:

[0135] In one aspect, a method of increasing the duration of use of a topical cosmetic composition comprises applying the cosmetic composition to a user and subsequently applying the topical cosmetic composition to the cosmetic composition.

[0136] In one aspect, the extract solution was evaluated for its effectiveness as an acetylcholine inhibitor.

[0137] In one aspect, silica particles preloaded with an extract solution are used to absorb sebum and release the extract solution as the sebum is absorbed.

[0138] In one aspect, Egyptian blue water lily extract and Chinese lotus extract are used in combination with a clinically effective amount of quercetin to produce antimicrobial and acetylcholine inhibitory effects.

[0139] In one aspect, increased color stability is seen compared to equivalent colorant loaded compositions lacking the extract.

[0140] On the one hand, the composition has good color stability even when wetted with sweat and sebum. It is appropriate to quantitatively measure the color stability by the lack of hue change, which can be measured by a color computer (SpectraFlash 600Plus; Datacolor International Company) as the ΔE value of the colorimetric data before and after silicone oil is applied to the composition and wetted until the point of maximum oil absorption. The Hunter Lab color system is used for such colorimetric measurements.

[0141] In one aspect, the extract is used in makeup or skincare setting sprays.

[0142] In one aspect, the extract is used for a time and in a concentration sufficient to produce an anti-inflammatory effect when applied repeatedly to the skin.

[0143] In one aspect, the extract is used in a topical composition that reduces pore size when applied for a sufficient time and concentration.

[0144] In one aspect, the extract is used in a topical composition that results in better subsequent adhesion of the cosmetic formulation prior to its application by reducing the presence of skin sebum.

[0145] aspect

[0146] In one aspect, the extract is incorporated into a powder form.

[0147] In one aspect, a cosmetic primer composition comprises an effective amount of an acetylcholine release inhibitor for reducing skin inflammation and / or pore size prior to application of a makeup composition.

Claims

1. A method for treating skin with an extract solution, the extract solution comprising: Egyptian blue water lily (nymphaea coerulea) flower extract; and Chinese lotus (nelumbo nucifera) flower extract, wherein the effectiveness of the component is determined by an assay for determining the level of sebum production, sebocyte leptin cell surface downregulation and / or acetylcholine release inhibition in a sample that has been contacted with the extract or a composition comprising the extract.

2. A method comprising: (1) applying a sebum reducing composition comprising a topically acceptable vehicle and a sufficient amount of a sebum reducing agent to the skin, followed by; (2) applying a cosmetic colorant composition comprising a colorant to the same skin, wherein sequential application of the sebum reducing composition and the cosmetic colorant composition results in increased wear of the cosmetic colorant composition on the skin. 3 . The method according to claim 2 , wherein the sebum reducing agent is an extract solution comprising an Egyptian blue water lily extract and a Chinese lotus extract.

4. A composition comprising silica particles loaded with an extract solution comprising an Egyptian blue water lily extract and a Chinese lotus extract; in, When the silica particles are applied to a substrate containing sebum, the silica particles are able to release the extract and preferably absorb the sebum.

5. The composition of claim 4, wherein the released extract reduces the amount of sebum produced by the substrate by at least 10% after 8 hours compared to the same substrate not contacted with the extract.

6. A composition according to any preceding claim, further comprising quercetin or another suitable antimicrobial material.

7. A cosmetic composition comprising an extract solution, wherein the extract solution comprises an Egyptian blue water lily extract and a Chinese lotus extract; wherein the makeup composition exhibits improved color stability when in contact with a sebum solution compared to an otherwise identical makeup composition lacking the extract solution.

8. The method of claim 2, wherein the sebum reducing composition is in the form of a spray.

9. The method of claim 1, wherein the skin is treated with the extract for a duration, frequency, and / or with a sufficient concentration to result in a decrease in expression of inflammatory biomarkers in the treated skin.

10. The method of claim 1, wherein the skin is treated with the extract for a duration, frequency, and / or with a sufficient concentration to result in a reduction in median pore size of the treated skin.

11. The method according to any one of the preceding claims, wherein the composition comprising the extract is in powder form.

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