Application of lime sulfur in prevention and treatment of ampullaria gigas
By using lithosulfur mixture, the effective concentration is ≥0.45%, which acts on the eggs of Fushou snails, which solves the problem of difficult to prevent and control the incubation of Fushou snails in the existing technology, and effectively protects aquatic crops.
Patent Information
- Application Number
- CN202411940397.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-26
- Publication Date
- 2025-04-29
AI Technical Summary
The prior art is difficult to effectively and safely prevent and control the hatching of Fushou snails, which makes it difficult to control the harm to aquatic crops.
Lithium sulfur mixture is used as the active ingredient and prepared into a water agent by mixing it with water. The effective concentration is ≥0.45%, and it acts on the Fushou snail eggs to prevent them from hatching.
Lithium sulfur mixture significantly reduces the hatching rate of Fushou snail eggs, reduces the harm to aquatic crops, and has low toxicity, no chemical residues, and is cheap.
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of field pest control, and in particular relates to the application of lime sulfur in controlling golden apple snails. Background Art
[0002] Pomacea canaliculata, also known as apple snail and big bottle snail, belongs to the phylum Mollusca, class Gastropoda, order Mesogastropoda, family Pomacea, genus Pomacea. It is an amphibious freshwater mollusk native to the Amazon River Basin in South America. Pomacea canaliculata was introduced for breeding in large quantities in the 1980s. However, due to the market benefits not meeting expectations and improper breeding management, coupled with the characteristics of strong adaptability, high reproduction rate and varied diet of Pomacea canaliculata, it has caused huge losses to the planting, fishery and breeding industries. Among them, rice (Oryza sativa L.) is the main crop harmed by Pomacea canaliculata, mainly manifested in the gnawing of seedlings and tillering seedlings, resulting in a decrease in the number of tillers and insufficient effective panicles, which can cause a 90% reduction in rice production.
[0003] At present, the field control of golden apple snails is mainly carried out through physical control, chemical control and biological control. Among them, physical methods include physical direct eradication, crushing or high-pressure water gun flushing. This method can directly eliminate golden apple snail eggs and solve the problem of golden apple snails harming crops from the source, but it is time-consuming and inefficient, and is not suitable for practical application in agricultural production. Chemical control often uses products such as snail killing amine and tetraacetaldehyde. This method has fast results, good effects, simple methods of use, and little seasonal restrictions. However, long-term and large-scale use not only has high economic costs, but also affects the growth of other non-target organisms, reduces biodiversity, causes environmental pollution and agricultural product safety problems, and also makes golden apple snails resistant to drugs, resulting in reduced killing effects. Biological control often uses methods such as raising ducks in rice fields, raising fish and turtles in rice fields; this type of method can avoid damaging the ecological environment and agricultural product pollution, but it has not been widely promoted due to its lack of specificity, high breeding costs and large manpower investment.
[0004] Pomfret usually lives in water, but prefers to lay eggs above the water surface. When the young snails hatch, they fall into the water and continue to grow and develop. Pomfret eggs are bright red. Newly laid Pomfret eggs are soft. The gelatinous mucus on the surface can form a layer of film after drying and solidification, which is essential for maintaining the normal shape of the egg, preventing water loss and ensuring the normal development of the embryo. Therefore, how to find low-toxic and safe compounds to destroy Pomfret eggs and prevent Pomfret hatching has become an urgent problem to be solved based on the composition of Pomfret eggs or egg shells. Summary of the invention
[0005] In view of the above problems existing in the prior art, the present invention has found through experimental screening that lime sulfur mixture can prevent the hatching of Pomacea canaliculata eggs, thereby reducing its harm to crops. The present invention is achieved through the following technical solutions.
[0006] Firstly, the present invention provides the application of lime sulfur mixture in preventing and controlling golden apple snails.
[0007] Furthermore, in the application, lime sulfur mixture is used as an active ingredient to act on the eggs of Pomacea canaliculata.
[0008] Furthermore, in the application, lime sulfur is mixed with water to prepare an aqueous solution.
[0009] Furthermore, in the aqueous solution, the effective concentration of lime sulfur is ≥0.45%.
[0010] Based on the above application, the present invention provides a method for controlling Pomacea canaliculata, which comprises: using lime sulfur mixture as an active ingredient to act on Pomacea canaliculata eggs.
[0011] Furthermore, in the method, lime sulfur is mixed with water to prepare an aqueous solution.
[0012] Furthermore, in the aqueous solution, the effective concentration of lime sulfur is ≥0.45%.
[0013] In the present invention, the mechanism by which lime sulfur kills golden apple snail eggs may include:
[0014] 1. Lime sulfur is an alkaline compound that may react with the protein in the eggshell, causing the protein to denature and inactivate, resulting in the inability of the golden apple snail eggs to hatch normally.
[0015] 2. Lime sulfur may react with carotenoids (astaxanthin) in the eggs of the golden apple snail, destroying the carotenoids and causing them to lose their protective effect on the contents of the eggs, thereby reducing the eggs' resistance to stress and causing them to fail to hatch normally.
[0016] 3. Lime sulfur may also react with hexagonal calcium carbonate in the eggshell, causing the eggs to no longer be supported and protected by the eggshell, making it impossible for the golden apple snail eggs to hatch normally and leading to death.
[0017] 4. Lime sulfur mixture may directly cause dehydration and death of golden apple snail eggs.
[0018] Compared with the prior art, the application of lime sulfur mixture in preventing and controlling golden apple snails has the following beneficial effects:
[0019] Lime sulfur itself has low toxicity, produces no chemical residue, is easy to obtain, and has low cost. It inactivates the eggs of Pomacea canaliculata through penetration, corrosion, etc., and has significant effects. It is a safe and efficient Pomacea canaliculata control agent. The present invention targets the components of Pomacea canaliculata eggs and uses lime sulfur as an effective ingredient to destroy the eggs of Pomacea canaliculata, so that the eggs of Pomacea canaliculata cannot be hatched normally, thereby playing a role in controlling Pomacea canaliculata.
[0020] The invention is of great significance for reducing the bite damage of golden apple snails to aquatic crops such as rice, lotus, wild rice stem, taro, water chestnut, water caltrop, gorgonian, water spinach, etc., and avoiding the reduction of aquatic crops due to the damage of golden apple snails.
[0021] The feces of the golden apple snail can cause water pollution and reduce the diversity of freshwater organisms. The present invention kills the golden apple snail eggs to achieve effective control of the golden apple snail, and also plays an important role in reducing water pollution caused by the golden apple snail.
[0022] The golden apple snail has many parasites and is a carrier of many parasitic diseases. Killing the golden apple snail eggs can effectively control the snail at the source, thereby greatly reducing the threat posed by the golden apple snail to human health.
[0023] Pomacea apple snails are mobile and can hide in waters. They are widely distributed and difficult to find, which requires the use of large amounts of chemical pesticides, which is costly and pollutes the environment. However, Pomacea apple snail eggs often gather together in piles. The technical solution of directly killing Pomacea apple snail eggs uses a small amount of pesticides, is low in cost, and avoids secondary pesticide application in the later stage, which can play a preventive role at the source. DETAILED DESCRIPTION
[0024] The present invention is explained below in conjunction with the embodiments, and the technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0025] The experimental methods in the following examples, unless otherwise specified, are all conventional methods, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The materials, reagents, etc. used in the following examples, unless otherwise specified, can all be obtained from commercial channels.
[0026] Firstly, the present invention provides the application of lime sulfur mixture in preventing and controlling golden apple snails.
[0027] Furthermore, in the application, lime sulfur mixture is used as an active ingredient to act on the eggs of Pomacea canaliculata.
[0028] Furthermore, in the application, lime sulfur is mixed with water to prepare an aqueous solution.
[0029] Furthermore, in the aqueous solution, the effective concentration of lime sulfur is ≥0.45%.
[0030] Based on the above application, the present invention provides a method for controlling Pomacea canaliculata, which comprises: using lime sulfur mixture as an active ingredient to act on Pomacea canaliculata eggs.
[0031] Furthermore, in the method, lime sulfur is mixed with water to prepare an aqueous solution.
[0032] Furthermore, in the aqueous solution, the effective concentration of lime sulfur is ≥0.45%.
[0033] In the following examples, the lime sulfur mixture was purchased from Henan Sanchuan Ruihe Crop Protection Co., Ltd., and the calcium polysulfide content in the lime sulfur mixture was 45%.
[0034] Example 1
[0035] This example describes the preparation method of Pomacea canaliculata ovicidal agent A.
[0036] Preparation steps: Prepare 1g of lime sulfur and 99g of water;
[0037] Mixing step: mixing the prepared lime sulfur mixture with water to dilute the lime sulfur mixture into a 1.00% aqueous solution, wherein the concentration of the active ingredient of the aqueous solution is 0.45%, thereby obtaining the Pomacea canaliculata ovicidal agent A.
[0038] Example 2
[0039] This example describes the preparation method of Pomacea canaliculata ovicidal agent B.
[0040] Preparation steps: Prepare 2g of lime sulfur and 98g of water;
[0041] Mixing step: mixing the prepared lime sulfur mixture with water to dilute the lime sulfur mixture into a 2.00% aqueous solution, wherein the concentration of the active ingredient of the aqueous solution is 0.90%, thereby obtaining the Pomacea canaliculata ovicidal agent B.
[0042] Example 3
[0043] This example describes the preparation method of Pomacea canaliculata ovicidal agent C.
[0044] Preparation steps: Prepare 3g of lime sulfur and 97g of water;
[0045] Mixing step: mixing the prepared lime sulfur mixture with water to dilute the lime sulfur mixture into a 3.00% aqueous solution, wherein the concentration of the active ingredient of the aqueous solution is 1.35%, thereby obtaining the Pomacea canaliculata egg-killing agent C.
[0046] Example 4
[0047] This example describes the preparation method of Pomacea canaliculata ovicidal agent D.
[0048] Preparation steps: Prepare 4g of lime sulfur and 96g of water;
[0049] Mixing step: mixing the prepared lime sulfur mixture with water to dilute the lime sulfur mixture into a 4.00% aqueous solution, wherein the concentration of the active ingredient of the aqueous solution is 1.80%, thereby obtaining the Pomacea canaliculata ovicidal agent D.
[0050] Example 5
[0051] This example describes the preparation method of Pomacea canaliculata ovicidal agent E.
[0052] Preparation steps: Prepare 5g of lime sulfur and 95g of water;
[0053] Mixing step: mixing the prepared lime sulfur mixture with water to dilute the lime sulfur mixture into a 5.00% aqueous solution, wherein the concentration of the active ingredient of the aqueous solution is 2.25%, thereby obtaining the Pomacea canaliculata ovicidal agent E.
[0054] Example 6
[0055] This example describes the indoor killing effect of the Pomacea canaliculata ovicide prepared by the method described in Examples 1 to 5.
[0056] The Pomacea canaliculata ovicides prepared in Examples 1 to 5 were respectively placed in sprayers for use. Several small storage boxes were prepared, each of which was connected to about half of the volume of tap water, and the box opening was covered with a nylon net. Ten nests of Pomacea canaliculata eggs were placed on the nylon net to simulate the field hatching environment of Pomacea canaliculata eggs.
[0057] The prepared Pomacea canaliculata ovicide was sprayed on the surface of the Pomacea canaliculata eggs until they were completely wet, and the storage box was placed in a constant temperature environment (30°C) to observe the hatching results of the eggs. Each group was repeated 3 times, and each repeated group contained 10 nests of Pomacea canaliculata eggs. The Pomacea canaliculata eggs were sprayed with clean water as a blank control.
[0058] The experiment was carried out for 45 days. If the hatching volume of the egg mass did not reach 1 / 5 or turned black and rotted after 45 days, it was considered dead. The mortality rate and corrected mortality rate were calculated. The experimental results are shown in Table 1.
[0059] Mortality rate = cumulative number of deaths / total number of experiments × 100%;
[0060] Adjusted mortality rate = [(mortality rate of treatment group - mortality rate of control group) / (1 - mortality rate of control group)] × 100%.
[0061] Table 1. Egg mass mortality of each group under indoor conditions
[0062] Ovicide Ovicide A Ovicide B Ovicide C Ovicide D Ovicide E Blank control mortality rate 13.33％ 16.67％ 43.33％ 100％ 100％ 6.67％ Adjusted mortality rate 7.14％ 10.71％ 39.28％ 100％ 100％ /
[0063] Results analysis: As shown in Table 1, after 45 days of incubation, the egg-killing effect of Pomacea canaliculata ovicides D and E was significantly better than that of Pomacea canaliculata ovicides A, B and C, and the corrected mortality rate reached 100%, with obvious results. Compared with the blank control, Pomacea canaliculata ovicides A, B and C all had an inhibitory effect on the hatching of Pomacea canaliculata eggs.
[0064] Based on the test results of this embodiment, it can be seen that when the active ingredient concentration of lime sulfur is not less than 1.80%, the killing effect on Pomacea canaliculata eggs can reach 100%. In practical applications, the concentration of lime sulfur can also be appropriately adjusted for considerations such as protecting the ecological environment and reducing costs.
[0065] Example 7
[0066] This example describes the field killing effect of the Pomacea canaliculata ovicide prepared by the method described in Examples 1 to 5.
[0067] Experimental location: Rice experimental field in Huihe Village, Wenjiang District, Chengdu City, Sichuan Province.
[0068] Trial time: May 2024.
[0069] Test method: A rice test field with the same rice growth was selected, no pesticides were applied to the rice test field, and there were unhatched Pomacea canaliculata egg masses on the rice stalks. Pomacea canaliculata egg-killing agent A, Pomacea canaliculata egg-killing agent B, Pomacea canaliculata egg-killing agent C, Pomacea canaliculata egg-killing agent D, and Pomacea canaliculata egg-killing agent E prepared by the methods described in Examples 1 to 5 were sprayed on the surface of the Pomacea canaliculata egg masses on the rice stalks until they were completely wetted. Each egg-killing agent was sprayed on 20 nests of egg masses, and each group was marked to observe the hatching results.
[0070] The test was carried out for 14 days (under natural environmental conditions, the hatching time of Pomacea canaliculata eggs is generally 7 to 14 days). If the hatching volume of the egg mass did not reach 1 / 5 or turned black and rotted after 14 days, it was considered dead. The mortality rate and the corrected mortality rate were calculated using the same calculation method as in Example 6. The test results are shown in Table 2.
[0071] Table 2. Egg mass mortality of each group under field conditions
[0072] Ovicide Ovicide A Ovicide B Ovicide C Ovicide D Ovicide E Blank control mortality rate 15.00％ 30.00％ 40.00％ 90.00％ 90.00％ 5.00％ Adjusted mortality rate 10.53％ 26.32％ 36.84％ 89.47％ 89.47％ /
[0073] Results analysis: As shown in Table 2, after 14 days of field trials, the killing effects of Pomacea canaliculata ovicides D and Pomacea canaliculata ovicides E were the best, reaching about 90%, significantly higher than Pomacea canaliculata ovicides A, B and C. Compared with the blank control, Pomacea canaliculata ovicides A, B and C all had an inhibitory effect on the hatching of Pomacea canaliculata eggs.
[0074] In summary, the present invention dilutes lime sulfur mixture with water to prepare an aqueous solution, and the aqueous solution shows a good killing effect of Pomacea canaliculata eggs both indoors and in the field. The technical solution provided by the present invention can prevent and control Pomacea canaliculata from the root, avoid its harm to crops such as rice, and has a good prospect of wide application.
[0075] The embodiments described above are only some embodiments of the present invention, not all embodiments. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but only represents selected embodiments of the present invention. All other embodiments obtained without creative work and related deductions and substitutions made by ordinary technicians in the field under the conditions of the concept of the present invention belong to the scope of protection of the present invention.
Claims
1. Application of lime sulfur mixture in controlling golden apple snails.
2. The use according to claim 1, characterized in that: Lime sulfur mixture is used as an active ingredient to act on the eggs of Pomacea canaliculata.
3. The use according to claim 2, characterized in that: Mix lime sulfur with water to prepare an aqueous solution.
4. The use according to claim 3, characterized in that: In the aqueous solution, the effective concentration of lime sulfur is ≥0.45%.
5. A method for controlling golden apple snails, characterized in that: The method comprises: using lime sulfur mixture as an active ingredient to act on the eggs of Pomacea canaliculata.
6. The method for controlling Pomacea canaliculata according to claim 5, characterized in that: Mix lime sulfur with water to prepare an aqueous solution.
7. The method for controlling Pomacea canaliculata according to claim 6, characterized in that: In the aqueous solution, the effective concentration of lime sulfur is ≥0.45%.