Plant growth regulator for improving stress resistance of crops

Abstract

The invention discloses a plant growth regulator for improving crop stress resistance, and belongs to the technical field of plant growth regulators. The black fungus polysaccharide wine is prepared from the following raw materials in parts by weight: 10-20 parts of modified black fungus polysaccharide, 30-50 parts of wine processing solid byproduct fermentation liquor, 0.05-0.15 part of compound sodium nitrophenolate, 1-3 parts of trisodium citrate, 1-3 parts of gamma-aminobutyric acid and 0.3-0.6 part of an accelerant. The accelerant is composed of nano chitosan and disodium cocoyl glutamate according to the mass ratio of 1: 5. After the prepared plant growth regulator is used, the stress resistance of crops can be remarkably improved, and the saline-alkali resistance, drought resistance and low-temperature stress resistance of the crops are improved.

Description

Technical Field

[0001] The invention belongs to the technical field of plant growth regulators, and in particular relates to a plant growth regulator capable of improving the stress resistance of crops. Background Art

[0002] With the intensification of global climate change and environmental degradation, crop stress resistance has become an important issue for sustainable agricultural development. Plants are often affected by various adversities during their growth, including biotic stress (pests and diseases, weeds, etc.) and abiotic stress (drought, high and low temperatures, high salinity, low light, heavy metals, etc.), which can cause damage to plants and even death in severe cases. The damage caused by adversity to plants is mainly manifested in membrane system damage, reactive oxygen damage, destruction of protective enzyme systems, dehydration of plant cells, decreased activity of photosynthetic enzymes, unstable respiration, and enhanced catabolism. Plants also develop adaptability and resistance to adverse environments during their growth and evolution, namely, plant stress resistance.

[0003] Salt-alkali stress usually includes two aspects: salt stress and alkali stress. Salt stress mainly refers to the stress caused to plants by neutral salts, mainly NaCl and Na2SO4; while alkali stress refers to the stress caused to plants by alkaline salts, mainly Na2CO3 and NaHCO3. Under salt-alkali stress, crop root cells will be harmed by osmotic stress and high pH, ​​leading to the deficiency of mineral elements such as phosphorus and magnesium in crops. The specific harm to crops is manifested in inhibiting the normal growth of crops, affecting the absorption of water and nutrients, directly destroying the plant cell structure, and destroying the soil structure, which seriously affects the yield and quality of crops.

[0004] The damage caused by low temperature stress includes damage to plants caused by low temperatures above 0℃ (i.e., chilling damage) and damage caused by low temperatures below 0℃ (i.e., freezing damage). Chilling damage slows down plant growth, destroys the water balance in the body, causes the plant to lose water and wilt; cell membrane permeability increases, organic matter leaks out, causing plant metabolic disorders; chlorophyll synthesis is inhibited, leaves turn yellow and lose green, photosynthetic enzyme activity is inhibited, photosynthetic rate decreases; protein synthesis decreases, and endogenous hormone content increases. Freezing damage includes extracellular freezing and intracellular freezing, which mainly causes damage to protoplasm through ice crystals, causing damage to the cell membrane system, loss of membrane selective permeability, organic matter leaks out, enzyme activity changes, and protein structure destruction; low temperature stress seriously affects the growth and development of crops.

[0005] Stressful environments seriously affect the yield and quality of crops, and the method of improving crop stress resistance by applying plant growth regulators is widely used in agricultural production. Plant growth regulators can regulate different stages in the growth and development of plants, such as promoting seed germination, rooting, flowering, fruiting, ripening, dormancy, etc., and can also improve crop quality and enhance the ability of plants to resist cold, drought, salinity and pests and diseases. Plant growth regulators are widely used in crop stress resistance cultivation due to their low cost, small dosage and significant effect. Currently, the commonly used plant growth regulators are mainly chemical synthetic substances, such as auxin, gibberellic acid, abscisic acid, sodium nitrophenolate, etc., but a single plant growth regulator often has a poor effect on regulating plant growth, especially the effect of enhancing crop stress resistance. Therefore, there is an urgent need for a plant growth regulator that can improve crop stress resistance in order to achieve the best regulation effect. Summary of the invention

[0006] The purpose of the present invention is to provide a plant growth regulator for improving the stress resistance of crops. Black fungus polysaccharide extracted by enzyme method is acidified and modified and then reacted with sodium selenite to obtain selenized black fungus polysaccharide. The introduction of selenium element improves the biological activity of black fungus polysaccharide. After being used in combination with other multiple components, the stress resistance of crops can be significantly improved, and the ability of crops to resist salt and alkali, drought and low temperature stress can be improved.

[0007] In order to achieve the above technical objectives, the technical solution adopted by the present invention is: A plant growth regulator for improving the stress resistance of crops is prepared from the following raw materials in parts by weight: 5-15 parts of modified black fungus polysaccharide, 20-30 parts of fermentation liquid of solid byproduct of wine processing, 0.05-0.15 parts of sodium nitrophenolate, 1-3 parts of trisodium citrate, 1-3 parts of gamma-aminobutyric acid, and 0.3-0.6 parts of an accelerator; the accelerator is composed of nano-chitosan and disodium cocoyl glutamate in a mass ratio of 1:5.

[0008] Preferably, the modified black fungus polysaccharide is prepared by the following method: (1) After drying black fungus, crush it and pass it through a 100-mesh sieve, add deionized water and black fungus powder according to the solid-liquid ratio, homogenize, adjust the pH to 4.0-4.5, add 5wt% complex enzyme solution, and hydrolyze for 2 hours. Then, increase the temperature to 55°C and adjust the pH to 6.0-6.5, then add 2% saccharifying enzyme, hydrolyze for 1 hour, and then adjust the pH to 6.5-7.0, add mannanase and neutral protease, and hydrolyze for 3-4 hours at 40-45°C. Inactivate the enzyme at high temperature to obtain an enzymatic solution; centrifuge the enzymatic solution, take the supernatant, add 3 times the amount of 95% ethanol, let it stand for 24 hours, and then centrifuge it at high speed, and dry the obtained precipitate to obtain black fungus polysaccharide; (2) 5 g of black fungus polysaccharide was dissolved in 200 mL of 2 mol / L sodium hydroxide solution, stirred until completely dissolved, then 1.5 wt% of chloroacetic acid was added, heated to 50 °C and stirred for 4 h, then cooled to room temperature to obtain a mixed solution, and the mixed solution was freeze-dried into powder to obtain acidified black fungus polysaccharide; (3) Acidified black fungus polysaccharide and sodium selenite are mixed in proportion to obtain a mixture, a 1% by volume nitric acid solution is added to the mixture to dissolve it completely, and then BaCl2 is added. The mixture is stirred evenly and reacted in a water bath at 70°C for 4-5 hours. After the reaction is completed, an appropriate amount of saturated sodium sulfate solution is added to remove barium chloride, and the supernatant is obtained by centrifugation. The pH value is adjusted to neutral, and 3 times the amount of 95% ethanol is added. The mixture is allowed to stand for 24 hours and then centrifuged at high speed. The obtained precipitate is dried to obtain modified black fungus polysaccharide.

[0009] Preferably, in step (1), the material-liquid ratio of black fungus powder to deionized water is 1 g:30 ml; the complex enzyme solution contains 10-15 g / L of cellulase and 15-20 g / L of pectinase; the added amount of mannanase is 0.5-1 g / L, and the added amount of neutral protease is 1.5-2 g / L.

[0010] Preferably, the enzyme activity of the cellulase is 5000 U / g, the enzyme activity of the pectinase is 40000 U / g; the enzyme activity of the saccharifying enzyme is 10000 U / g, the enzyme activity of the mannanase is 50000 U / g, and the enzyme activity of the neutral protease is 60000 U / g.

[0011] Preferably, in step (3), the mass ratio of acidified black fungus polysaccharide to sodium selenite is 3:1, and the dosage ratio of the 1% nitric acid solution, the mixture, BaCl2, and the saturated sodium sulfate solution is 50ml:1g:0.2g:3-5ml.

[0012] Preferably, the wine processing solid by-product fermentation liquid is prepared by the following method: a. Dry the solid byproducts of wine processing and crush them through an 80-mesh sieve to obtain a solid powder, add a composite hydrolase solution thereto, and hydrolyze at 35-40°C for 6-8h, inactivate at high temperature, and obtain an enzymatic solution; b. Add Bacillus licheniformis and Lactobacillus plantarum to the enzymatic hydrolysate and adjust the temperature to 30-35°C for fermentation for 24 hours. Filter and take the filtrate.

[0013] Preferably, in step a, the dosage ratio of the solid powder to the composite hydrolase solution is 1 g: 1.5-2 ml.

[0014] Preferably, the composite hydrolase solution contains 10-15 g / L of cellulase and 20-25 g / L of bromelain; the enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the bromelain is 100,000 U / g.

[0015] Preferably, in step b, the amount of Bacillus licheniformis added is 10-15 g / L, and the amount of Lactobacillus plantarum added is 20-30 g / L.

[0016] Preferably, the viable bacterial count of Bacillus licheniformis is 100 billion / g; the viable bacterial count of Lactobacillus plantarum is 10 billion / g.

[0017] Unless otherwise specified, the raw materials used in the present invention are all existing commercially available products, among which nano-chitosan was purchased from Xi'an Qiyue Biotechnology Co., Ltd. with a particle size of 100-150 nm; Bacillus licheniformis was purchased from Guangxi Nongbao Bioengineering Co., Ltd.; and Lactobacillus plantarum was purchased from Shandong Pingao Pharmaceutical Co., Ltd.

[0018] The plant growth regulator for improving the stress resistance of crops obtained by the present invention is a liquid agent, which can be mixed with fertilizer or used alone. When mixed, the dosage is 2-3% of the fertilizer mass; when used alone, it can be diluted and sprayed or root-applied to crops, and the dosage is 10kg / mu.

[0019] Solid byproducts of wine processing mainly include discarded grape skins, seeds, pectin and tartar, etc., as well as dead yeast cells, peel fragments, pulp particles and other solid substances. Traditionally, these solid waste residues are discharged into landfills or used as organic fertilizers. However, these methods not only burden the environment, but may also spread pathogens and chemical residues. The present invention performs enzymatic hydrolysis and fermentation treatment. First, the enzymatic hydrolysis process decomposes complex organic matter into simpler small molecules such as sugars and amino acids. On the one hand, these substances are more easily absorbed and utilized by microorganisms for fermentation, thereby producing richer and more biologically active metabolites, which are effective in enhancing the stress resistance of crops; on the other hand, these substances can be directly absorbed and utilized by crops, promoting the growth and development of crops and improving the stress resistance of crops.

[0020] The modified black fungus polysaccharide used in the present invention is selenized black fungus polysaccharide. The introduction of selenium improves the biological activity of the black fungus polysaccharide and has a more significant effect on promoting plant growth. When it is used in combination with the fermentation liquid of the solid byproduct of wine processing, the two act synergistically and significantly improve the ability of crops to resist saline-alkali, drought and low temperature stress.

[0021] The promoter of the invention contains nano-chitosan and disodium cocoyl glutamate in a specific ratio, and plays a role in promoting the penetration and absorption of multiple active substances. The nano-chitosan can also be used as a carrier to load a small amount of incompletely decomposed anthocyanidins contained in the fermentation liquid of the solid byproduct of wine processing onto the surface of plants, and the anthocyanidins can be better absorbed and utilized by the plants through the penetration-promoting performance of the disodium cocoyl glutamate, thereby optimizing the nutrient distribution and physiological metabolic process in the plant body, thereby improving the stress resistance and yield of the plant.

[0022] The beneficial effects of the present invention are as follows: the present invention utilizes solid byproducts of wine processing for fermentation and utilization, realizes the recycling of resources, can not only reduce the discharge of waste, reduce environmental pollution, and improve the economic benefits of enterprises; after the black fungus polysaccharide is modified and used in combination with the fermentation liquid of solid byproducts of wine processing, the two act synergistically, and significantly improve the ability of crops to resist saline-alkali, drought, and low temperature stress. The plant growth regulator prepared by the present invention has the advantages of being safer and having fewer side effects. It can act on crops as a plant growth regulator or foliar fertilizer, effectively activate the plant's defense mechanism, alleviate the damage of crops caused by abiotic stress, improve stress resistance, and improve crop quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The SOD enzyme activity of rapeseed seedlings in Test Example 3 of the present invention; Figure 2 The CAT enzyme activity of rapeseed seedlings in Test Example 3 of the present invention; Figure 3 This is the POD enzyme activity of rapeseed seedlings in Test Example 3 of the present invention. DETAILED DESCRIPTION

[0024] The technical solution of the present invention is further described below in conjunction with specific embodiments, but is not limited thereto.

[0025] Example 1 A plant growth regulator for improving the stress resistance of crops is prepared from the following raw materials in parts by weight: 5kg of modified black fungus polysaccharide, 20kg of fermentation liquid of solid byproduct of wine processing, 0.05kg of sodium nitrophenolate, 1kg of trisodium citrate, 1kg of gamma-aminobutyric acid, and 0.3kg of an accelerator; the accelerator is composed of nano-chitosan and disodium cocoyl glutamate in a mass ratio of 1:5.

[0026] The modified black fungus polysaccharide is prepared by the following method: (1) Take black fungus, dry it, grind it through a 100-mesh sieve, add deionized water and black fungus powder according to a material-liquid ratio of 30 ml:1 g, homogenize, adjust the pH to 4.0, add 5 wt% complex enzyme solution, hydrolyze for 2 hours, increase the temperature to 55°C and adjust the pH to 6.0, then add 2% saccharifying enzyme, hydrolyze for 1 hour, then adjust the pH to 6.5, add mannanase and neutral protease, hydrolyze at 40°C for 3-4 hours, and kill the enzyme at high temperature to obtain an enzymatic solution; centrifuge the enzymatic solution, take the supernatant, add 3 times the amount of 95% ethanol, let it stand for 24 hours, and then centrifuge it at high speed, and dry the obtained precipitate to obtain black fungus polysaccharide; The complex enzyme solution contains 10 g / L of cellulase and 15 g / L of pectinase; the added amount of mannanase is 0.5 g / L, and the added amount of neutral protease is 1.5 g / L; the enzyme activity of the cellulase is 5000 U / g, and the enzyme activity of the pectinase is 40000 U / g; the enzyme activity of the saccharifying enzyme is 10000 U / g, the enzyme activity of the mannanase is 50000 U / g, and the enzyme activity of the neutral protease is 60000 U / g; (2) 5 g of black fungus polysaccharide was dissolved in 200 mL of 2 mol / L sodium hydroxide solution, stirred until completely dissolved, then 1.5 wt% of chloroacetic acid was added, heated to 50 °C and stirred for 4 h, then cooled to room temperature to obtain a mixed solution, and the mixed solution was freeze-dried into powder to obtain acidified black fungus polysaccharide; (3) Acidified black fungus polysaccharide and sodium selenite are mixed in a mass ratio of 3:1 to obtain a mixture, a 1% by volume nitric acid solution is added to the mixture to dissolve it completely, and then BaCl2 is added. The mixture is stirred evenly and reacted in a water bath at 70°C for 4-5 hours. After the reaction is completed, an appropriate amount of saturated sodium sulfate solution is added to remove barium chloride, and the supernatant is obtained by centrifugation. The pH value is adjusted to neutral, and 3 times the amount of 95% ethanol is added. After standing for 24 hours, the mixture is centrifuged at high speed, and the obtained precipitate is dried to obtain modified black fungus polysaccharide. The amount ratio of the 1% by volume nitric acid solution, the mixture, BaCl2, and the saturated sodium sulfate solution is 50ml:1g:0.2g:3ml.

[0027] The wine processing solid byproduct fermentation liquid is prepared by the following method: a. Dry the solid byproducts of wine processing and crush them through an 80-mesh sieve to obtain a solid powder, add a composite hydrolase solution thereto, and hydrolyze at 35°C for 8h, inactivate at high temperature, and obtain an enzymatic solution; b. Add Bacillus licheniformis and Lactobacillus plantarum to the enzymatic hydrolysate, adjust the temperature to 30°C, ferment for 24 hours, then filter and obtain the filtrate.

[0028] The dosage ratio of the solid powder to the composite hydrolase solution in step a is 1 g:1.5 ml.

[0029] The composite hydrolase solution contains 10 g / L of cellulase and 20 g / L of bromelain; the enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the bromelain is 100,000 U / g.

[0030] In the step b, the amount of Bacillus licheniformis added is 10 g / L, and the amount of Lactobacillus plantarum added is 20 g / L; the number of live bacteria of Bacillus licheniformis is 100 billion / g; the number of live bacteria of Lactobacillus plantarum is 10 billion / g.

[0031] Example 2 A plant growth regulator for improving the stress resistance of crops is prepared from the following raw materials in parts by weight: 15 kg of modified black fungus polysaccharide, 30 kg of fermentation liquid of solid byproduct of wine processing, 0.15 kg of sodium nitrophenolate, 3 kg of trisodium citrate, 3 kg of gamma-aminobutyric acid, and 0.6 kg of an accelerator; the accelerator is composed of nano-chitosan and disodium cocoyl glutamate in a mass ratio of 1:5.

[0032] The modified black fungus polysaccharide is prepared by the following method: (1) Take black fungus, dry it, grind it through a 100-mesh sieve, add deionized water and black fungus powder according to a material-liquid ratio of 30 ml:1 g, homogenize, adjust the pH to 4.5, add 5 wt% complex enzyme solution, hydrolyze for 2 hours, increase the temperature to 55°C and adjust the pH to 6.5, then add 2% saccharifying enzyme, hydrolyze for 1 hour, then adjust the pH to 7.0, add mannanase and neutral protease, hydrolyze at 45°C for 3-4 hours, and kill the enzyme at high temperature to obtain an enzymatic solution; centrifuge the enzymatic solution, take the supernatant, add 3 times the amount of 95% ethanol, let it stand for 24 hours, and then centrifuge it at high speed, and dry the obtained precipitate to obtain black fungus polysaccharide; The complex enzyme solution contains 15 g / L of cellulase and 20 g / L of pectinase; the added amount of mannanase is 1 g / L, and the added amount of neutral protease is 2 g / L; the enzyme activity of the cellulase is 5000 U / g, and the enzyme activity of the pectinase is 40000 U / g; the enzyme activity of the saccharifying enzyme is 10000 U / g, the enzyme activity of the mannanase is 50000 U / g, and the enzyme activity of the neutral protease is 60000 U / g; (2) 5 g of black fungus polysaccharide was dissolved in 200 mL of 2 mol / L sodium hydroxide solution, stirred until completely dissolved, then 1.5 wt% of chloroacetic acid was added, heated to 50 °C and stirred for 4 h, then cooled to room temperature to obtain a mixed solution, and the mixed solution was freeze-dried into powder to obtain acidified black fungus polysaccharide; (3) Acidified black fungus polysaccharide and sodium selenite are mixed in a mass ratio of 3:1 to obtain a mixture, a 1% by volume nitric acid solution is added to the mixture to dissolve it completely, and then BaCl2 is added. The mixture is stirred evenly and reacted in a water bath at 70°C for 4-5 hours. After the reaction, an appropriate amount of saturated sodium sulfate solution is added to remove barium chloride, and the supernatant is obtained by centrifugation. The pH value is adjusted to neutral, and 3 times the amount of 95% ethanol is added. After standing for 24 hours, the mixture is centrifuged at high speed, and the obtained precipitate is dried to obtain modified black fungus polysaccharide. The amount ratio of the 1% by volume nitric acid solution, the mixture, BaCl2, and the saturated sodium sulfate solution is 50ml:1g:0.2g:5ml.

[0033] The wine processing solid byproduct fermentation liquid is prepared by the following method: a. Dry the solid byproducts of wine processing and crush them through an 80-mesh sieve to obtain a solid powder, add a composite hydrolase solution thereto, enzymolyze at 40°C for 6h, and inactivate at high temperature to obtain an enzymolysis solution; b. Add Bacillus licheniformis and Lactobacillus plantarum to the enzymatic hydrolysate, adjust the temperature to 35°C, ferment for 24 hours, then filter and obtain the filtrate.

[0034] In the step a, the dosage ratio of the solid powder to the composite hydrolase solution is 1 g: 2 ml.

[0035] The composite hydrolase solution contains 15 g / L of cellulase and 25 g / L of bromelain; the enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the bromelain is 100,000 U / g.

[0036] In the step b, the amount of Bacillus licheniformis added is 15 g / L, and the amount of Lactobacillus plantarum added is 30 g / L; the number of live bacteria of Bacillus licheniformis is 100 billion / g; the number of live bacteria of Lactobacillus plantarum is 10 billion / g.

[0037] Example 3 A plant growth regulator for improving the stress resistance of crops is prepared from the following raw materials in parts by weight: 10 kg of modified black fungus polysaccharide, 25 kg of fermentation liquid of solid byproduct of wine processing, 0.1 kg of sodium nitrophenolate, 2 kg of trisodium citrate, 2 kg of gamma-aminobutyric acid, and 0.5 kg of an accelerator; the accelerator is composed of nano-chitosan and disodium cocoyl glutamate in a mass ratio of 1:5.

[0038] The modified black fungus polysaccharide is prepared by the following method: (1) Take black fungus, dry it, grind it through a 100-mesh sieve, add deionized water and black fungus powder according to a material-liquid ratio of 30 ml:1 g, homogenize, adjust the pH to 4.5, add 5 wt% complex enzyme solution, hydrolyze for 2 hours, increase the temperature to 55°C and adjust the pH to 6.0, then add 2% saccharifying enzyme, hydrolyze for 1 hour, then adjust the pH to 7.0, add mannanase and neutral protease, hydrolyze at 45°C for 3-4 hours, and kill the enzyme at high temperature to obtain an enzymatic solution; centrifuge the enzymatic solution, take the supernatant, add 3 times the amount of 95% ethanol, let it stand for 24 hours, and then centrifuge it at high speed, and dry the obtained precipitate to obtain black fungus polysaccharide; The complex enzyme solution contains 13 g / L of cellulase and 18 g / L of pectinase; the added amount of mannanase is 0.8 g / L, and the added amount of neutral protease is 1.8 g / L; the enzyme activity of the cellulase is 5000 U / g, and the enzyme activity of the pectinase is 40000 U / g; the enzyme activity of the saccharifying enzyme is 10000 U / g, the enzyme activity of the mannanase is 50000 U / g, and the enzyme activity of the neutral protease is 60000 U / g; (2) 5 g of black fungus polysaccharide was dissolved in 200 mL of 2 mol / L sodium hydroxide solution, stirred until completely dissolved, then 1.5 wt% of chloroacetic acid was added, heated to 50 °C and stirred for 4 h, then cooled to room temperature to obtain a mixed solution, and the mixed solution was freeze-dried into powder to obtain acidified black fungus polysaccharide; (3) Acidified black fungus polysaccharide and sodium selenite are mixed in a mass ratio of 3:1 to obtain a mixture, a nitric acid solution with a volume fraction of 1% is added to the mixture to dissolve it completely, and then BaCl2 is added. The mixture is stirred evenly and reacted in a water bath at 70°C for 4-5 hours. After the reaction, an appropriate amount of saturated sodium sulfate solution is added to remove barium chloride, and the supernatant is obtained by centrifugation. The pH value is adjusted to neutral, and 3 times the amount of 95% ethanol is added. After standing for 24 hours, the mixture is centrifuged at high speed. The obtained precipitate is dried to obtain modified black fungus polysaccharide. The usage ratio of the nitric acid solution with a volume fraction of 1%, the mixture, BaCl2, and the saturated sodium sulfate solution is 50ml:1g:0.2g:4ml.

[0039] The wine processing solid byproduct fermentation liquid is prepared by the following method: a. Dry the solid byproducts of wine processing and crush them through an 80-mesh sieve to obtain a solid powder, add a composite hydrolase solution thereto, enzymolyze at 40°C for 6h, and inactivate at high temperature to obtain an enzymolysis solution; b. Add Bacillus licheniformis and Lactobacillus plantarum to the enzymatic hydrolysate, adjust the temperature to 35°C, ferment for 24 hours, then filter and obtain the filtrate.

[0040] In the step a, the dosage ratio of the solid powder to the composite hydrolase solution is 1 g: 2 ml.

[0041] The composite hydrolase solution contains 12 g / L of cellulase and 23 g / L of bromelain; the enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the bromelain is 100,000 U / g.

[0042] In the step b, the amount of Bacillus licheniformis added is 12 g / L, and the amount of Lactobacillus plantarum added is 25 g / L; the number of live bacteria of Bacillus licheniformis is 100 billion / g; the number of live bacteria of Lactobacillus plantarum is 10 billion / g.

[0043] Example 4 A plant growth regulator for improving the stress resistance of crops is prepared from the following raw materials in parts by weight: 12 kg of modified black fungus polysaccharide, 25 kg of fermentation liquid of solid byproduct of wine processing, 0.08 kg of sodium nitrophenolate, 1.5 kg of trisodium citrate, 1.5 kg of gamma-aminobutyric acid, and 0.4 kg of an accelerator; the accelerator is composed of nano-chitosan and disodium cocoyl glutamate in a mass ratio of 1:5.

[0044] The modified black fungus polysaccharide is prepared by the following method: (1) Take black fungus, dry it, grind it through a 100-mesh sieve, add deionized water and black fungus powder according to a material-liquid ratio of 30 ml:1 g, homogenize, adjust the pH to 4.0, add 5 wt% complex enzyme solution, hydrolyze for 2 hours, increase the temperature to 55°C and adjust the pH to 6.0, then add 2% saccharifying enzyme, hydrolyze for 1 hour, then adjust the pH to 7.0, add mannanase and neutral protease, hydrolyze at 45°C for 3-4 hours, and kill the enzyme at high temperature to obtain an enzymatic solution; centrifuge the enzymatic solution, take the supernatant, add 3 times the amount of 95% ethanol, let it stand for 24 hours, and then centrifuge it at high speed, and dry the obtained precipitate to obtain black fungus polysaccharide; The complex enzyme solution contains 15 g / L of cellulase and 20 g / L of pectinase; the added amount of mannanase is 1 g / L, and the added amount of neutral protease is 1.5 g / L; the enzyme activity of the cellulase is 5000 U / g, and the enzyme activity of the pectinase is 40000 U / g; the enzyme activity of the saccharifying enzyme is 10000 U / g, the enzyme activity of the mannanase is 50000 U / g, and the enzyme activity of the neutral protease is 60000 U / g; (2) 5 g of black fungus polysaccharide was dissolved in 200 mL of 2 mol / L sodium hydroxide solution, stirred until completely dissolved, then 1.5 wt% of chloroacetic acid was added, heated to 50 °C and stirred for 4 h, then cooled to room temperature to obtain a mixed solution, and the mixed solution was freeze-dried into powder to obtain acidified black fungus polysaccharide; (3) Acidified black fungus polysaccharide and sodium selenite are mixed in a mass ratio of 3:1 to obtain a mixture, a nitric acid solution with a volume fraction of 1% is added to the mixture to dissolve it completely, and then BaCl2 is added. The mixture is stirred evenly and reacted in a water bath at 70°C for 4-5 hours. After the reaction, an appropriate amount of saturated sodium sulfate solution is added to remove barium chloride, and the supernatant is obtained by centrifugation. The pH value is adjusted to neutral, and 3 times the amount of 95% ethanol is added. After standing for 24 hours, the mixture is centrifuged at high speed. The obtained precipitate is dried to obtain modified black fungus polysaccharide. The usage ratio of the nitric acid solution with a volume fraction of 1%, the mixture, BaCl2, and the saturated sodium sulfate solution is 50ml:1g:0.2g:4ml.

[0045] The wine processing solid byproduct fermentation liquid is prepared by the following method: a. Dry the solid byproducts of wine processing and crush them through an 80-mesh sieve to obtain a solid powder, add a composite hydrolase solution thereto, enzymolyze at 40°C for 8h, and inactivate at high temperature to obtain an enzymolysis solution; b. Add Bacillus licheniformis and Lactobacillus plantarum to the enzymatic hydrolysate, adjust the temperature to 35°C, ferment for 24 hours, then filter and obtain the filtrate.

[0046] The dosage ratio of the solid powder to the composite hydrolase solution in step a is 1 g:1.5 ml.

[0047] The composite hydrolase solution contains 15 g / L of cellulase and 20 g / L of bromelain; the enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the bromelain is 100,000 U / g.

[0048] In the step b, the amount of Bacillus licheniformis added is 15 g / L, and the amount of Lactobacillus plantarum added is 20 g / L; the number of viable bacteria of the Bacillus licheniformis is 100 billion / g; the number of viable bacteria of the Lactobacillus plantarum is 10 billion / g.

[0049] Comparative Example 1 A plant growth regulator for improving the stress resistance of crops is prepared from the following raw materials in parts by weight: 12 kg of black fungus polysaccharide, 25 kg of fermentation liquid of solid byproduct of wine processing, 0.08 kg of sodium nitrophenolate, 1.5 kg of trisodium citrate, 1.5 kg of gamma-aminobutyric acid, and 0.4 kg of an accelerator; the accelerator is composed of nano-chitosan and disodium cocoyl glutamate in a mass ratio of 1:5.

[0050] The black fungus polysaccharide is prepared by the following method: taking black fungus, drying it, crushing it through a 100-mesh sieve, adding deionized water and black fungus powder according to a material-liquid ratio of 30 ml: 1 g, homogenizing, adjusting the pH to 4.0, adding 5wt% of a composite enzyme solution, performing enzymolysis for 2 hours, raising the temperature to 55°C and adjusting the pH to 6.0, adding 2% of a saccharifying enzyme, performing enzymolysis for 1 hour, and then adjusting the pH to 7.0, adding mannanase and neutral protease, performing enzymolysis at 45°C for 3-4 hours, and killing the enzymes at high temperature to obtain an enzymolysis solution; centrifuging the enzymolysis solution, taking the supernatant, adding 3 times the amount of 95% ethanol, standing for 24 hours, and then centrifuging at high speed, and drying the obtained precipitate to obtain the black fungus polysaccharide; The complex enzyme solution contains 15 g / L of cellulase and 20 g / L of pectinase; the added amount of the mannanase is 1 g / L, and the added amount of the neutral protease is 1.5 g / L; the enzyme activity of the cellulase is 5000 U / g, and the enzyme activity of the pectinase is 40000 U / g; the enzyme activity of the saccharifying enzyme is 10000 U / g, the enzyme activity of the mannanase is 50000 U / g, and the enzyme activity of the neutral protease is 60000 U / g.

[0051] The wine processing solid byproduct fermentation liquid is prepared by the following method: a. Dry the solid byproducts of wine processing and crush them through an 80-mesh sieve to obtain a solid powder, add a composite hydrolase solution thereto, enzymolyze at 40°C for 8h, and inactivate at high temperature to obtain an enzymolysis solution; b. Add Bacillus licheniformis and Lactobacillus plantarum to the enzymatic hydrolysate, adjust the temperature to 35°C, ferment for 24 hours, then filter and obtain the filtrate.

[0052] The dosage ratio of the solid powder to the composite hydrolase solution in step a is 1 g:1.5 ml.

[0053] The composite hydrolase solution contains 15 g / L of cellulase and 20 g / L of bromelain; the enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the bromelain is 100,000 U / g.

[0054] In the step b, the amount of Bacillus licheniformis added is 15 g / L, and the amount of Lactobacillus plantarum added is 20 g / L; the number of viable bacteria of the Bacillus licheniformis is 100 billion / g; the number of viable bacteria of the Lactobacillus plantarum is 10 billion / g.

[0055] The raw material composition and preparation method in the above comparative example are basically the same as those in Example 4, with the only difference being that black fungus polysaccharide is used instead of modified black fungus polysaccharide.

[0056] Comparative Example 2 A plant growth regulator for improving the stress resistance of crops is prepared from the following raw materials in parts by weight: 25 kg of fermentation liquid of solid byproduct of wine processing, 0.08 kg of sodium nitrophenolate, 1.5 kg of trisodium citrate, 1.5 kg of gamma-aminobutyric acid, and 0.4 kg of an accelerator; the accelerator is composed of nano-chitosan and disodium cocoyl glutamate in a mass ratio of 1:5.

[0057] The wine processing solid byproduct fermentation liquid is prepared by the following method: a. Dry the solid byproducts of wine processing and crush them through an 80-mesh sieve to obtain a solid powder, add a composite hydrolase solution thereto, enzymolyze at 40°C for 8h, and inactivate at high temperature to obtain an enzymolysis solution; b. Add Bacillus licheniformis and Lactobacillus plantarum to the enzymatic hydrolysate, adjust the temperature to 35°C, ferment for 24 hours, then filter and obtain the filtrate.

[0058] The dosage ratio of the solid powder to the composite hydrolase solution in step a is 1 g:1.5 ml.

[0059] The composite hydrolase solution contains 15 g / L of cellulase and 20 g / L of bromelain; the enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the bromelain is 100,000 U / g.

[0060] In the step b, the amount of Bacillus licheniformis added is 15 g / L, and the amount of Lactobacillus plantarum added is 20 g / L; the number of viable bacteria of the Bacillus licheniformis is 100 billion / g; the number of viable bacteria of the Lactobacillus plantarum is 10 billion / g.

[0061] The raw material composition and preparation method in the above comparative example are basically the same as those in Example 4, the only difference being that modified black fungus polysaccharide is not contained.

[0062] Comparative Example 3 A plant growth regulator for improving the stress resistance of crops is prepared from the following raw materials in parts by weight: 12 kg of modified black fungus polysaccharide, 0.08 kg of sodium nitrophenolate, 1.5 kg of trisodium citrate, 1.5 kg of gamma-aminobutyric acid, and 0.4 kg of an accelerator; the accelerator is composed of nano-chitosan and disodium cocoyl glutamate in a mass ratio of 1:5.

[0063] The modified black fungus polysaccharide is prepared by the following method: (1) Take black fungus, dry it, grind it through a 100-mesh sieve, add deionized water and black fungus powder according to a material-liquid ratio of 30 ml:1 g, homogenize, adjust the pH to 4.0, add 5 wt% complex enzyme solution, hydrolyze for 2 hours, increase the temperature to 55°C and adjust the pH to 6.0, then add 2% saccharifying enzyme, hydrolyze for 1 hour, then adjust the pH to 7.0, add mannanase and neutral protease, hydrolyze at 45°C for 3-4 hours, and kill the enzyme at high temperature to obtain an enzymatic solution; centrifuge the enzymatic solution, take the supernatant, add 3 times the amount of 95% ethanol, let it stand for 24 hours, and then centrifuge it at high speed, and dry the obtained precipitate to obtain black fungus polysaccharide; The complex enzyme solution contains 15 g / L of cellulase and 20 g / L of pectinase; the added amount of mannanase is 1 g / L, and the added amount of neutral protease is 1.5 g / L; the enzyme activity of the cellulase is 5000 U / g, and the enzyme activity of the pectinase is 40000 U / g; the enzyme activity of the saccharifying enzyme is 10000 U / g, the enzyme activity of the mannanase is 50000 U / g, and the enzyme activity of the neutral protease is 60000 U / g; (2) 5 g of black fungus polysaccharide was dissolved in 200 mL of 2 mol / L sodium hydroxide solution, stirred until completely dissolved, then 1.5 wt% of chloroacetic acid was added, heated to 50 °C and stirred for 4 h, then cooled to room temperature to obtain a mixed solution, and the mixed solution was freeze-dried into powder to obtain acidified black fungus polysaccharide; (3) Acidified black fungus polysaccharide and sodium selenite are mixed in a mass ratio of 3:1 to obtain a mixture, a nitric acid solution with a volume fraction of 1% is added to the mixture to dissolve it completely, and then BaCl2 is added. The mixture is stirred evenly and reacted in a water bath at 70°C for 4-5 hours. After the reaction, an appropriate amount of saturated sodium sulfate solution is added to remove barium chloride, and the supernatant is obtained by centrifugation. The pH value is adjusted to neutral, and 3 times the amount of 95% ethanol is added. After standing for 24 hours, the mixture is centrifuged at high speed. The obtained precipitate is dried to obtain modified black fungus polysaccharide. The usage ratio of the nitric acid solution with a volume fraction of 1%, the mixture, BaCl2, and the saturated sodium sulfate solution is 50ml:1g:0.2g:4ml.

[0064] The raw material composition and preparation method in the above comparative example are basically the same as those in Example 4, except that the fermentation liquid of the solid by-product of wine processing is not contained.

[0065] Comparative Example 4 A plant growth regulator for improving the stress resistance of crops is prepared from the following raw materials in parts by weight: 12 kg of modified black fungus polysaccharide, 25 kg of fermentation liquid of solid byproduct of wine processing, 0.08 kg of sodium nitrophenolate, 1.5 kg of trisodium citrate and 1.5 kg of gamma-aminobutyric acid.

[0066] The modified black fungus polysaccharide is prepared by the following method: (1) Take black fungus, dry it, grind it through a 100-mesh sieve, add deionized water and black fungus powder according to a material-liquid ratio of 30 ml:1 g, homogenize, adjust the pH to 4.0, add 5 wt% complex enzyme solution, hydrolyze for 2 hours, increase the temperature to 55°C and adjust the pH to 6.0, then add 2% saccharifying enzyme, hydrolyze for 1 hour, then adjust the pH to 7.0, add mannanase and neutral protease, hydrolyze at 45°C for 3-4 hours, and kill the enzyme at high temperature to obtain an enzymatic solution; centrifuge the enzymatic solution, take the supernatant, add 3 times the amount of 95% ethanol, let it stand for 24 hours, and then centrifuge it at high speed, and dry the obtained precipitate to obtain black fungus polysaccharide; The complex enzyme solution contains 15 g / L of cellulase and 20 g / L of pectinase; the added amount of mannanase is 1 g / L, and the added amount of neutral protease is 1.5 g / L; the enzyme activity of the cellulase is 5000 U / g, and the enzyme activity of the pectinase is 40000 U / g; the enzyme activity of the saccharifying enzyme is 10000 U / g, the enzyme activity of the mannanase is 50000 U / g, and the enzyme activity of the neutral protease is 60000 U / g; (2) 5 g of black fungus polysaccharide was dissolved in 200 mL of 2 mol / L sodium hydroxide solution, stirred until completely dissolved, then 1.5 wt% of chloroacetic acid was added, heated to 50 °C and stirred for 4 h, then cooled to room temperature to obtain a mixed solution, and the mixed solution was freeze-dried into powder to obtain acidified black fungus polysaccharide; (3) Acidified black fungus polysaccharide and sodium selenite are mixed in a mass ratio of 3:1 to obtain a mixture, a nitric acid solution with a volume fraction of 1% is added to the mixture to dissolve it completely, and then BaCl2 is added. The mixture is stirred evenly and reacted in a water bath at 70°C for 4-5 hours. After the reaction, an appropriate amount of saturated sodium sulfate solution is added to remove barium chloride, and the supernatant is obtained by centrifugation. The pH value is adjusted to neutral, and 3 times the amount of 95% ethanol is added. After standing for 24 hours, the mixture is centrifuged at high speed. The obtained precipitate is dried to obtain modified black fungus polysaccharide. The usage ratio of the nitric acid solution with a volume fraction of 1%, the mixture, BaCl2, and the saturated sodium sulfate solution is 50ml:1g:0.2g:4ml.

[0067] The wine processing solid byproduct fermentation liquid is prepared by the following method: a. Dry the solid byproducts of wine processing and crush them through an 80-mesh sieve to obtain a solid powder, add a composite hydrolase solution thereto, enzymolyze at 40°C for 8h, and inactivate at high temperature to obtain an enzymolysis solution; b. Add Bacillus licheniformis and Lactobacillus plantarum to the enzymatic hydrolysate, adjust the temperature to 35°C, ferment for 24 hours, then filter and obtain the filtrate.

[0068] The dosage ratio of the solid powder to the composite hydrolase solution in step a is 1 g:1.5 ml.

[0069] The composite hydrolase solution contains 15 g / L of cellulase and 20 g / L of bromelain; the enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the bromelain is 100,000 U / g.

[0070] In the step b, the amount of Bacillus licheniformis added is 15 g / L, and the amount of Lactobacillus plantarum added is 20 g / L; the number of viable bacteria of the Bacillus licheniformis is 100 billion / g; the number of viable bacteria of the Lactobacillus plantarum is 10 billion / g.

[0071] The raw material composition and preparation method in the above comparative example are basically the same as those in Example 4, except that no accelerant is contained.

[0072] Test Example 1 Effect of the present invention on improving the ability of rapeseed seedlings to resist salt-alkali stress The experiment was conducted in a greenhouse potted method, and the test site was an agricultural planting base in Liaocheng City. Rapeseed seeds were sown in a hole pot mixed with nutrient soil and vermiculite in a ratio of 1:1, and cultured in a culture room at a temperature of 25°C for two weeks to obtain rapeseed seedlings. Select rapeseed seedlings with consistent growth and transplant them into pots (10 cm long × 10 cm × 12 cm high) containing 500 g of test soil (alkaline nitrogen content 88.55 mg / kg, available phosphorus content 37.23 mg / kg, available potassium content 107.62 mg / kg, organic matter content 20.19 g / kg, pH 6.55). The fertilization level of the potted soil (N-P2O5-K2O) was 20-10-20, and one plant was transplanted per pot. After transplanting, each pot was watered with 200 mL of water, and then each pot was watered with 100 mL of water every other day. Treatment was started 20 days after seedling transplantation. A total of 9 treatment groups were set up in the experiment, of which groups 1-4 were respectively treatment groups of embodiments 1-4, groups 5-8 were treatment groups of comparative examples 1-4, and group 9 was a blank control group; the specific treatment method of each treatment group was: on the 20th day after seedling transplantation, 100ml saline alkali solution + 0.15g of plant growth regulator obtained from different treatment groups was used for root irrigation treatment, wherein 100ml saline alkali solution was composed of 50ml 0.1mol / LNaCl+50ml 0.15mol / L NaHCO3 solution, and 9 groups were blank control groups replaced with 0.15g deionized water. After 10 days of root irrigation, the plant height, fresh weight, dry weight, and chlorophyll content of the plants in each treatment group were measured and statistically analyzed, and the test was repeated 4 times to take the average value. The test results are shown in Table 1.

[0073] Table 1 Results of the test on salt-alkali stress resistance of rapeseed in different treatment groups From the results in Table 1 above, it can be seen that compared with the blank control group, the salt-alkali stress alleviation effect of rapeseed seedlings treated with the plant growth regulators for improving crop stress resistance prepared in Examples 1-4 of the present invention is significantly better than that of other comparative examples 1-4, which indicates that the modified black fungus polysaccharide used in the raw material composition of the present invention is selenized black fungus polysaccharide, and the introduction of selenium improves the biological activity of black fungus polysaccharide, and has a significant effect on promoting plant growth. After being used in combination with the fermentation liquid of the solid by-product of wine processing, the two act synergistically, and the salt-alkali resistance of crops is significantly improved.

[0074] Test Example 2 Effect of the present invention on improving strawberry's ability to resist low temperature and chilling damage The test site is located in Liaocheng, Shandong Province. The potted experiment was carried out. The test crop was 2-leaf strawberry. The test soil for the potted plant was collected from the 0-20cm tillage layer of local representative dryland. The pot size was 10cm×12cm, with 500g of soil, and the fertilization level of the potted soil (N-P2O5-K2O) was 20-10-15. The 2-leaf stage red strawberry seedlings with consistent growth were transplanted into the above-mentioned pots, one plant was transplanted into each pot, and different treatments were started 15 days after transplanting. A total of 9 treatment groups were set up in the experiment and numbered 1-9. The pots of groups 1-9 were placed in a 4°C incubator for culture, and different test group solutions were sprayed / root-applied at the same time, wherein groups 1-4 respectively applied the plant growth regulators obtained in Examples 1-4, and groups 5-8 respectively applied the plant growth regulators obtained in Comparative Examples 1-4, with a usage and dosage of 0.15 g / pot, which could be sprayed directly or after adding an appropriate amount of water, and the spraying frequency was 5 days / time, wherein group 9 was a blank control group, and an equal amount of clean water was sprayed each time. The strawberry growth test conditions were the same as those of other treatment groups. Fifteen days after spraying, the growth of strawberries was observed and the height of plants in each treatment group was measured. Then, the strawberry plants were withered in an oven at 105°C for 30 min and then dried at 75°C to constant weight. The aboveground biomass and root biomass were measured. The test was repeated four times and the average value was taken. The specific test results are shown in Table 2.

[0075] Table 2 Results of strawberry low temperature stress test in different treatment groups It can be seen from the data in Table 2 that the plant height, aboveground biomass and underground biomass of groups 1-4 are significantly higher than those of the blank control group 9, and are also better than the comparative example 1-4 treatment groups. This shows that the plant growth regulator for improving crop stress resistance of the present invention can also significantly promote the growth and development of strawberries under low temperature conditions, and significantly improves the ability of strawberries to resist low temperature damage.

[0076] Test Example 3 Effects of different accelerator compositions of the present invention on the ability of rapeseed seedlings to resist drought stress The experiment was conducted in a greenhouse potted method at an agricultural planting base in Liaocheng City. Rapeseed seeds were sown in a hole pot mixed with nutrient soil and vermiculite in a ratio of 1:1. After two weeks of cultivation in a culture room at a temperature of 25°C, rapeseed seedlings were obtained. Selected rapeseed seedlings with consistent growth were transplanted into pots (10 cm long × 10 cm × 12 cm high) containing 500 g of test soil (alkaline nitrogen content 88.55 mg / kg, available phosphorus content 37.23 mg / kg, available potassium content 107.62 mg / kg, organic matter content 20.19 g / kg, pH 6.55). The fertilization level of the potted soil (N-P2O5-K2O) was 20-10-20, and one plant was transplanted per pot. Moderate drought treatment was started 10 days after seedling transplanting. A total of 6 treatment groups were set up in the experiment, of which Group 1 was a blank control group, i.e., a moderate drought treatment group. The soil moisture content was 45%-55% of the soil mass. The water consumption was calculated by weighing every other day and the corresponding amount of clean water was supplemented to maintain the soil moisture content; Groups 2-6 were treated with plant growth regulators 1-5, i.e., after moderate drought treatment (same as the treatment method for Group 1), plant growth regulators 1-5 solution were sprayed / root-applied, and the usage and dosage was 0.15g / pot. The plant growth regulator 1 is the product obtained in Example 4; the plant growth regulator 2 is basically the same as Example 4, except that the promoter is composed of nano-chitosan and disodium cocoyl glutamate in a mass ratio of 1:1; the plant growth regulator 3 is basically the same as Example 4, except that the promoter is composed of nano-chitosan and disodium cocoyl glutamate in a mass ratio of 1:3; the plant growth regulator 4 is basically the same as Example 4, except that the promoter is composed of nano-chitosan and disodium cocoyl glutamate in a mass ratio of 1:7; the plant growth regulator 5 is basically the same as Example 4, except that the promoter is composed of nano-chitosan and disodium cocoyl glutamate in a mass ratio of 1:9. After 10 days of sprinkler irrigation, the plant height, fresh weight, dry weight, and chlorophyll content of the plants in each treatment group were measured and statistically analyzed, and the test was repeated 4 times to take the average value. The test results are shown in Table 3.

[0077] Table 3 Effects of different accelerator compositions on the ability of rapeseed seedlings to resist drought stress The present invention also measured the activity of defense enzymes in the leaves of rapeseed seedlings in the above-mentioned different treatment groups. The specific operation method is: weigh 0.1 g of rapeseed leaves in each treatment group, first add 3 ml PBS (pH 7.8), grind and homogenize in an ice bath, then add 2 mL PBS to rinse the mortar, mix well, pour into a centrifuge tube and centrifuge at 10000r / min at 4°C for 20 min. The supernatant is the crude enzyme solution, which can be used for enzyme activity determination. The contents of SOD, CAT and POD in the crude enzyme extract were determined respectively. The experiment was repeated 4 times and the average value was taken. The superoxide dismutase (SOD) was determined by the nitroblue tetrazolium photoreduction method; the catalase (CAT) and peroxidase (POD) were determined by ultraviolet spectrophotometry. The specific results are as follows Figure 1-3 As shown above. Figure 1-3 It can be seen from the results that the composition ratio of the promoter of the present invention has a significant effect on the activities of SOD, CAT and POD enzymes in rapeseed seedlings, and only the promoter composed of the proportion of the present invention has the best effect on improving the activity of defense enzymes in rapeseed seedlings. This is because the promoter of the present invention promotes the penetration and absorption of various active substances, optimizes the nutrient distribution and physiological metabolic process in the plant, thereby improving the plant's stress resistance and yield.

[0078] It should be noted that the above embodiments are only partial embodiments of the preferred methods of implementing the present invention, rather than all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

Claims

1. A plant growth regulator for improving the stress resistance of crops, characterized in that: The invention is prepared from the following raw materials in parts by weight: 10-20 parts of modified black fungus polysaccharide, 30-50 parts of fermentation liquid of solid byproduct of wine processing, 0.05-0.15 parts of sodium nitrophenolate, 1-3 parts of trisodium citrate, 1-3 parts of gamma-aminobutyric acid and 0.3-0.6 parts of an accelerator; the accelerator is composed of nano-chitosan and disodium cocoyl glutamate in a mass ratio of 1:

5.

2. The plant growth regulator for improving crop stress resistance according to claim 1, characterized in that: The modified black fungus polysaccharide is prepared by the following method: (1) After drying black fungus, crush it and pass it through a 100-mesh sieve, add deionized water and black fungus powder according to the solid-liquid ratio, homogenize, adjust the pH to 4.0-4.5, add 5wt% complex enzyme solution, and hydrolyze for 2 hours. Then, increase the temperature to 55°C and adjust the pH to 6.0-6.5, then add 2% saccharifying enzyme, hydrolyze for 1 hour, and then adjust the pH to 6.5-7.0, add mannanase and neutral protease, and hydrolyze for 3-4 hours at 40-45°C. Inactivate the enzyme at high temperature to obtain an enzymatic solution; centrifuge the enzymatic solution, take the supernatant, add 3 times the amount of 95% ethanol, let it stand for 24 hours, and then centrifuge it at high speed, and dry the obtained precipitate to obtain black fungus polysaccharide; (2) 5 g of black fungus polysaccharide was dissolved in 200 mL of 2 mol / L sodium hydroxide solution, stirred until completely dissolved, then 1.5 wt% of chloroacetic acid was added, heated to 50 °C and stirred for 4 h, then cooled to room temperature to obtain a mixed solution, and the mixed solution was freeze-dried into powder to obtain acidified black fungus polysaccharide; (3) Acidified black fungus polysaccharide and sodium selenite are mixed in proportion to obtain a mixture, a 1% by volume nitric acid solution is added to the mixture to dissolve it completely, and then BaCl2 is added. The mixture is stirred evenly and reacted in a water bath at 70°C for 4-5 hours. After the reaction is completed, an appropriate amount of saturated sodium sulfate solution is added to remove barium chloride, and the supernatant is obtained by centrifugation. The pH value is adjusted to neutral, and 3 times the amount of 95% ethanol is added. The mixture is allowed to stand for 24 hours and then centrifuged at high speed. The obtained precipitate is dried to obtain modified black fungus polysaccharide.

3. The plant growth regulator for improving crop stress resistance according to claim 2, characterized in that: In the step (1), the material-liquid ratio of black fungus powder to deionized water is 1g:30ml; the complex enzyme solution contains 10-15g / L of cellulase and 15-20g / L of pectinase; the added amount of mannanase is 0.5-1g / L, and the added amount of neutral protease is 1.5-2g / L.

4. The plant growth regulator for improving crop stress resistance according to claim 3, characterized in that: The enzyme activity of the cellulase is 5000 U / g, the enzyme activity of the pectinase is 40000 U / g; the enzyme activity of the saccharifying enzyme is 10000 U / g, the enzyme activity of the mannanase is 50000 U / g, and the enzyme activity of the neutral protease is 60000 U / g.

5. The plant growth regulator for improving crop stress resistance according to claim 1, characterized in that: In the step (3), the mass ratio of acidified black fungus polysaccharide to sodium selenite is 3:1, and the dosage ratio of the 1% nitric acid solution, the mixture, BaCl2, and the saturated sodium sulfate solution is 50ml:1g:0.2g:3-5ml.

6. The plant growth regulator for improving crop stress resistance according to claim 1, characterized in that: The wine processing solid byproduct fermentation liquid is prepared by the following method: a. Dry the solid byproducts of wine processing and crush them through an 80-mesh sieve to obtain a solid powder, add a composite hydrolase solution thereto, and hydrolyze at 35-40°C for 6-8h, inactivate at high temperature, and obtain an enzymatic solution; b. Add Bacillus licheniformis and Lactobacillus plantarum to the enzymatic hydrolysate and adjust the temperature to 30-35°C for fermentation for 24 hours. Filter and take the filtrate.

7. The plant growth regulator for improving crop stress resistance according to claim 6, characterized in that: In the step a, the dosage ratio of the solid powder to the composite hydrolase solution is 1 g: 1.5-2 ml.

8. The plant growth regulator for improving crop stress resistance according to claim 6, characterized in that: The composite hydrolase solution contains 10-15 g / L of cellulase and 20-25 g / L of bromelain; the enzymatic activity of the cellulase is 5000 U / g, and the enzymatic activity of the bromelain is 100,000 U / g.

9. The plant growth regulator for improving crop stress resistance according to claim 6, characterized in that: In the step b, the amount of Bacillus licheniformis added is 10-15 g / L, and the amount of Lactobacillus plantarum added is 20-30 g / L.

10. The plant growth regulator for improving crop stress resistance according to claim 6, characterized in that: The number of live bacteria of Bacillus licheniformis is 100 billion / g; the number of live bacteria of Lactobacillus plantarum is 10 billion / g.

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