Preparation method of spirulina edible enzyme and filtering device thereof

Abstract

The invention discloses a preparation method of spirulina edible ferment in the technical field of spirulina, and the preparation method comprises the following steps: S1, putting fresh spirulina into a filtering device by adopting a floating spirulina filtering method, and draining until no water drops, so as to form spirulina mud; s2, preparing the following raw materials: 200 to 300g of algae mud, 500 to 800g of water, 30 to 50g of edible brown sugar and 1 to 3g of strain stock solution; the preparation method comprises the following steps: completely dissolving brown sugar in water, adding dry algae mud and a strain stock solution into the sugar water, loading into a barrel, and carrying out sealed fermentation; s3, opening the sealed plastic barrel every 24 hours, uniformly stirring, and timely sealing; s4, fermenting at the temperature of 15-30 DEG C for 3 months; and S5, performing outdoor fermentation for 3 months, and then performing sampling and observation. According to the scheme, the spirulina fermentation cost can be reduced, and the quality of the spirulina edible ferment can be improved.

Description

Technical Field

[0001] The invention relates to the technical field of spirulina, and in particular to a preparation method of spirulina edible enzyme and a filtering device thereof. Background Art

[0002] Spirulina, as a cyanobacteria, is well-known for its rich nutritional value. It contains a large amount of protein, vitamins (such as B vitamins), minerals, polysaccharides, and a variety of antioxidants. In addition, spirulina is also rich in various enzymes, which play an important role in promoting digestion and enhancing immunity. The enzymes in spirulina include but are not limited to superoxide dismutase (SOD), catalase (CAT), protease, lipase, etc., which are of great significance to maintaining human health.

[0003] Enzymes are a type of biological catalyst produced by living cells that has a high catalytic efficiency for its specific substrate. In the human body, enzymes are involved in almost all biochemical reactions and are essential for food digestion and absorption, energy conversion, immune regulation and other functions. As people pay more and more attention to health, the market demand for natural, safe and reliable edible enzymes is growing.

[0004] In the existing technical literature, there are indeed a lot of research and applications on the use of spirulina as a raw material to prepare fermentation enzymes. However, most existing research and industrial practices tend to mix spirulina with other ingredients for fermentation. Specifically, common practices include but are not limited to the following: 1. Fermentation of spirulina with grains, 2. Fermentation assisted by additives. The spirulina enzyme produced in this way not only affects the quality of the spirulina enzyme, but also has a high manufacturing cost. Summary of the invention

[0005] The present invention aims to provide a method for preparing spirulina edible enzyme and a filtering device thereof, so as to solve the problem of high cost in the prior art.

[0006] The method for preparing edible spirulina enzyme in the scheme comprises the following steps: S1: placing fresh spirulina in a 300-mesh filter screen by a floating algae filtration method, washing with water, filtering the washed spirulina in a filter device until no water is dripping, and forming algae mud;

[0007] S2: comprising the following raw materials: 200-300g of algae mud, 500-800g of water, 30-50g of edible brown sugar, and 1-3g of bacterial stock solution; firstly dissolve the brown sugar completely into the water, then add the dry algae mud and bacterial stock solution into the sugar water, and put it into a barrel for sealed fermentation;

[0008] S3: Starting from the second day after sealing, open the sealed plastic barrel every 24 hours to stir evenly and seal it in time. Continue to operate for 3 to 5 days. Then open the barrel to release air for 5 to 10 minutes every day. Continue to operate for 5 to 7 days. After the release is completed, stir once every 10 days, and continue to stir twice;

[0009] S4: Ferment for 3 months at an air temperature of 15-30°C. If the temperature is below 15°C, extend the fermentation time. If the temperature exceeds 30°C, shade treatment is used.

[0010] S5: Take samples for observation after 3 months of outdoor fermentation. If the enzyme is orange-red, emits a sour aroma, and has white colonies floating on the surface, transfer the enzyme to a ceramic jar for continued fermentation. The ceramic jar needs to be stored indoors away from light for at least 3 months.

[0011] Working principle and beneficial effects of this solution: 1. Using a filter can ensure that the spirulina is fully cleaned while retaining its intact cell structure. After cleaning, the spirulina needs to be drained as much as possible to form algae mud, which helps to control the proportion of raw materials in subsequent steps; algae mud, water, brown sugar and bacterial stock solution are the basic components of enzymes. 2. Brown sugar provides a carbon source for microorganisms to promote their growth and reproduction; while the bacterial stock solution introduces a specific microbial community to start the fermentation process. When mixing these raw materials, ensure that the brown sugar is completely dissolved in the water so that nutrients are evenly distributed to support microbial activity. 3. Frequent stirring at the beginning of fermentation helps oxygen enter the culture medium, which is essential for the rapid proliferation of aerobic microorganisms. Subsequently, reducing the stirring frequency and regularly venting is to gradually create an anaerobic environment, which is conducive to the production of specific metabolites, such as organic acids. 4. Appropriate temperature is very important to maintain an ideal fermentation rate. If the temperature is too low, the fermentation rate will slow down; if the temperature is too high, it may affect the activity of certain microorganisms or cause unnecessary side reactions. Therefore, control the temperature and ferment at 15-30℃ for 3 months. If the temperature is below 15℃, extend the fermentation time. If it exceeds 30℃, shade treatment is used. 5. After the initial fermentation, the color, smell and surface characteristics of the enzyme can be used to evaluate the fermentation status. Orange-red, sour aroma and white colonies usually indicate successful fermentation. Transferring the enzyme to a ceramic jar for further fermentation can improve its quality, and storing it away from light can prevent adverse changes caused by light.

[0012] Furthermore, the water content of the algae mud is 87-92%.

[0013] Furthermore, the filtering device comprises a filter screen with a mesh size of 300. The use of a filter screen with a mesh size of 300 can ensure that the spirulina is fully cleaned while retaining its complete cell structure.

[0014] Furthermore, the filtering device comprises a base, and two symmetrical filtering elements for reciprocating scraping are arranged on the base, a scraping plate is arranged between the two filtering elements, and the filter screen is located below the scraping plate.

[0015] Further, the filter element includes a fixed frame, the fixed frame is provided with a power member and a turbine, the power member includes a limit plate, the limit plate is provided with a through slot, a mounting block is horizontally slidably connected in the through slot, the scraper plate is fixedly connected between the two mounting blocks, the scraper plate contacts the filter screen, the mounting block is connected with a driving member that drives it to move horizontally, a displacement slot is provided on the mounting block, a limit block is rotatably connected in the mounting block, a worm is provided at the bottom of the limit block, the worm is meshed with the turbine, a positioning block is fixedly connected to the limit block, the mounting block is fixedly connected to the fixed block, a tension spring is provided between the fixed block and the positioning block, a supporting protrusion is provided in the displacement slot for limiting the limit block to move only in the direction where the positioning block is provided, driving gears are provided at both ends of the turbine, the driving gear is meshed with a driven gear, the driven gear is connected with a rotating shaft, the filter screen is wound around the rotating shaft, and the rotating shaft is rotatably connected to the fixed frame. When in use, place the spirulina on the filter screen and turn on the driving part. The driving part drives the installation block to move axially back and forth. The movement of the installation block drives the limit block to move. Therefore, the installation block and the limit block will reciprocate horizontally in the through groove in the limit plate. After the limit block moves horizontally, it will drive the worm connected to the limit block to move horizontally. When the worm moves forward reciprocatingly, the worm approaches the turbine. When passing through the turbine, the limit block will tilt under the action of the force. The positioning block on the limit block will tilt with it, and the tension spring will play a limiting role to prevent the limit block from tilting too much. The worm moves forward after passing the turbine and will not drive the turbine to rotate; when the worm moves back horizontally and approaches the turbine, it is held by the groove of the turbine. Under the action of the holding protrusion, the limit block cannot rotate, and the worm moves while driving the turbine. The rotation of the turbine will drive the driven gear to rotate through the active gear, thereby driving the shaft to rotate, and then driving the filter on the shaft to rotate, thereby tightening the filter, and the scraper plate will scrape the filter with the reciprocating movement of the mounting block, accelerating the rapid filtration of spirulina on the filter. After the filtration is completed, the algae mud can be scooped out with the scraper plate.

[0016] Since Spirulina is rich in protein, vitamins, minerals and other nutrients, if it is soaked in water for a long time, these nutrients may dissolve into the water, causing nutrient loss. Rapid water filtration can reduce this loss. In addition, if the water filtration process is too long, in a suitable water environment, microorganisms such as bacteria and mold can easily multiply in large numbers and contaminate Spirulina. Rapid water filtration can reduce the risk of microbial growth. In addition, long-term soaking will cause Spirulina to absorb too much water, resulting in a certain impact on the algae structure, which may cause it to break. Rapid water filtration helps maintain its morphological integrity and ensure product quality.

[0017] Furthermore, the driving member includes a screw rod, the mounting block is threadedly connected to the screw rod, the screw rod is connected to a stepper motor, and the stepper motor is fixedly connected to the fixing frame. The forward and reverse rotation of the stepper motor controls the forward and reverse rotation of the screw rod, thereby controlling the reciprocating horizontal displacement of the mounting block.

[0018] Furthermore, the driving member is a cylinder, which is more convenient.

[0019] Furthermore, the fixing frame includes two symmetrical fixing plates, a connecting shaft is rotatably connected between the fixing plates, the turbine is fixedly connected to the connecting shaft, a plurality of positioning grooves are arranged on one side of the connecting shaft, a connecting block is fixedly connected to the fixing plate, the connecting block penetrates and is slidably connected to a limiting shaft, one end of the limiting shaft is clamped in the positioning groove, a baffle is arranged at the other end of the limiting shaft, a limiting spring is arranged between the baffle and the connecting block, and the limiting spring is sleeved on the limiting shaft. When the turbine rotates, the positioning groove rotates, squeezing the limiting shaft, and the limiting shaft stretches the limiting spring, so that the limiting shaft is away from the positioning groove. When the turbine does not rotate, the limiting shaft is clamped in the positioning groove, which can limit the connecting shaft and prevent the connecting shaft from rotating. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a structural schematic diagram of a method for preparing edible spirulina enzyme and a filtering device thereof of the present invention;

[0021] Figure 2 for Figure 1 Left side view of the filter element

[0022] Figure 3 for Figure 1 The other side of the filter element is attempted;

[0023] Figure 4 for Figure 3 A magnified view of middle;

[0024] Figure 5 for Figure 2 Side view of the connecting block. DETAILED DESCRIPTION

[0025] The following is a further detailed description through specific implementation methods:

[0026] The figure marks in the drawings of the specification include: base 1, fixed plate 2, connecting block 3, baffle 4, driving gear 5, positioning groove 6, connecting shaft 7, turbine 8, worm 9, limit plate 10, mounting block 11, limit block 12, fixed block 13, scraper plate 14, reinforcement plate 15, tension spring 16, positioning block 17, screw 18, through groove 19, motor 20, filter screen 21, driven gear 22, rotating shaft 23, wastewater tank 24, limit shaft 25, limit spring 26, supporting protrusion 111, and missing groove 141.

[0027] Embodiment 1 is basically as attached Figures 1 to 5 As shown: A method for preparing edible spirulina enzyme, comprising the following steps: S1: placing fresh spirulina in a 300-mesh filter 21 by a floating algae filtration method, washing with water, filtering the washed spirulina in a filter device until no water is dripping, and forming algae mud;

[0028] The filtering device comprises a base 1, on which are two symmetrical filtering elements for reciprocating scraping;

[0029] The filter element comprises two symmetrical fixed plates 2, a connecting shaft 7 is rotatably connected between the two fixed plates 2, a turbine 8 is fixedly connected to the connecting shaft 7, six positioning grooves 6 are arranged on one side of the connecting shaft 7, a connecting block 3 is fixedly connected to the fixed plate 2 by bolts, a limiting shaft 25 is passed through and slidably connected to the connecting block 3, one end of the limiting shaft 25 is clamped in the positioning groove 6, a baffle 4 is arranged at the other end of the limiting shaft 25, a limiting spring 26 is arranged between the baffle 4 and the connecting block 3, and the limiting spring 26 is sleeved on the limiting shaft 25; a driving gear 5 is fixedly connected to both ends of the turbine 8, the driving gear 5 is meshed with a driven gear 22, the driven gear 22 is fixedly connected to a rotating shaft 23, a filter screen 21 is wound around the rotating shaft 23, and both ends of the rotating shaft 23 are rotatably connected between the two fixed plates 2,

[0030] A limit plate 10 is also fixedly connected between the two fixed plates 2. The limit plate 10 is located above the connecting shaft 7. A horizontally arranged through groove 19 is provided in the limit plate 10. A mounting block 11 is horizontally slidably connected in the through groove 19. A reinforcing plate 15 is fixedly connected between the two mounting blocks 11 respectively located on the two filter elements. A scraper plate 14 is fixedly connected to the bottom of the reinforcing plate 15. The scraper plate 14 is made of silicone material. The filter screen 21 is located below the scraper plate 14, and the scraper plate 14 contacts the surface of the filter screen 21. The mounting block 11 is connected to a driving member that drives it to move horizontally. The driving member includes a screw 18. The mounting block 11 is threadedly connected to the screw 18. The screw 1 8 is connected with a stepper motor 20, which is fixedly connected to the fixing frame, a displacement groove is provided on the mounting block 11, a limit block 12 is rotatably connected in the mounting block 11, a worm 9 is fixedly connected to the bottom of the limit block 12, the worm 9 is meshed with the turbine 8, a positioning block 17 is fixedly connected to the limit block 12, a fixed block 13 is fixedly connected to the mounting block 11, a tension spring 16 is provided between the fixed block 13 and the positioning block 17, a supporting protrusion 111 is provided in the displacement groove for limiting the limit block 12 to move only in the direction where the positioning block 17 is provided, and a missing groove 141 matching the supporting protrusion 111 is provided on one side of the limit block 12 close to the supporting protrusion 111;

[0031] A wastewater pool 24 may be provided below the filter screen 21 to prevent the filtered water from soiling the workshop floor.

[0032] S2: comprising the following raw materials: 200-300g of algae mud, the water content of which is 87-92%, 500-800g of water, 30-50g of edible brown sugar, and 1-3g of bacterial stock solution; firstly dissolve the brown sugar completely in water, then add the dry algae mud and bacterial stock solution into the sugar water, and then put them into a barrel and seal them for fermentation;

[0033] S3: Starting from the second day after sealing, open the sealed plastic barrel every 24 hours to stir evenly and seal it in time. Continue to operate for 3 to 5 days. Then open the barrel to release air for 5 to 10 minutes every day. Continue to operate for 5 to 7 days. After the release is completed, stir once every 10 days, and continue to stir twice;

[0034] S4: Ferment for 3 months at an air temperature of 15-30°C. If the temperature is below 15°C, extend the fermentation time. If the temperature exceeds 30°C, shade treatment is used.

[0035] S5: Take samples for observation after 3 months of outdoor fermentation. If the enzyme is orange-red, emits a sour aroma, and has white colonies floating on the surface, transfer the enzyme to a ceramic jar for continued fermentation. The ceramic jar needs to be stored indoors away from light for at least 3 months.

[0036] 1. Using the filter 21 can ensure that the spirulina is fully cleaned while retaining its complete cell structure. After cleaning, the spirulina needs to be drained as much as possible to form algae mud, which is helpful for controlling the proportion of raw materials in subsequent steps. Since spirulina is rich in protein, vitamins, minerals and other nutrients, these nutrients may dissolve into the water if soaked in water for a long time, causing nutrient loss. Rapid water filtration can reduce this loss. In addition, if the water filtration process takes too long, in a suitable water environment, microorganisms such as bacteria and molds are likely to multiply in large numbers and contaminate the spirulina. Rapid water filtration can reduce the risk of microbial growth. In addition, long-term soaking will cause the spirulina to absorb too much water, resulting in a certain impact on the algae structure, which may cause breakage. Rapid water filtration helps to maintain its morphology intact and ensure product quality.

[0037] Therefore, the present application adopts a self-developed filtering device for rapid filtration.

[0038] Before using the filtering device, first place the spirulina on the filter screen 21, and control the forward and reverse rotation of the screw 18 by the stepping motor 20, thereby controlling the reciprocating horizontal displacement of the mounting block 11. The reciprocating horizontal movement of the mounting block 11 will drive the limit block 12 to move, so the mounting block 11 and the limit block 12 will both reciprocate horizontally in the through groove 19 in the limit plate 10. After the limit block 12 moves horizontally, it will drive the worm 9 connected to the limit block 12 to move horizontally. When the worm 9 moves back and forth forward (that is, from left to right as shown in the figure), the worm 9 approaches the turbine 8. When the worm 9 passes through the turbine 8, the limit block 12 is blocked by the turbine 8 and tilted to the lower right. The positioning block 17 on the limit block 12 tilts with it, and the tension spring 16 plays a limiting role, so that the limit block 12 can return to its original state after the limit block 12 is no longer subjected to force, and also avoids The limit block 12 is prevented from tilting too much. After the worm 9 tilts, it will not abut the turbine 8, and will not drive the turbine 8 to rotate; and when the worm 9 moves back horizontally (that is, from right to left as shown in the figure), the worm 9 is close to the turbine 8 and is abutted by the groove of the turbine 8. Under the action of the abutting protrusion 111, the abutting protrusion 111 and the missing groove 141 cooperate to limit the limit block 12 from rotating. When the worm 9 moves horizontally, it will drive the turbine 8 to rotate. The rotation of the turbine 8 will drive the driven gear 22 to rotate through the driving gear 5, thereby driving the rotation of the rotating shaft 23, and then driving the filter screen 21 on the rotating shaft 23 to rotate, thereby tightening the filter screen 21, and the scraper plate 14 will scrape the filter screen 21 with the reciprocating movement of the mounting block 11, accelerate the rapid filtration of spirulina on the filter screen 21, and use the scraper plate 14 to scoop out the algae mud after the filtration is completed.

[0039] Algae mud, water, brown sugar and bacterial stock solution are the basic components of enzymes. 2. Brown sugar provides a carbon source for microorganisms to promote their growth and reproduction; while the bacterial stock solution introduces a specific microbial community to start the fermentation process. When mixing these raw materials, ensure that the brown sugar is completely dissolved in the water so that nutrients are evenly distributed to support microbial activity. 3. Frequent stirring at the beginning of fermentation helps oxygen enter the culture medium, which is essential for the rapid proliferation of aerobic microorganisms. Subsequently, reducing the stirring frequency and regularly venting is to gradually create an anaerobic environment, which is conducive to the production of specific metabolites, such as organic acids. 4. Appropriate temperature is very important for maintaining an ideal fermentation rate. If the temperature is too low, the fermentation rate will slow down; if the temperature is too high, it may affect the activity of certain microorganisms or cause unnecessary side reactions. Therefore, control the temperature and ferment at an air temperature of 15 to 30°C for 3 months. If the temperature is below 15°C, extend the fermentation time, and shade treatment is used if it exceeds 30°C. 5. After initial fermentation, the color, smell and surface characteristics of the enzyme can be used to evaluate the fermentation status. Orange-red, sour aroma and white colonies usually indicate successful fermentation. Transferring the enzyme to a ceramic jar for further fermentation can improve its quality, and storing it away from light can prevent adverse changes caused by light.

[0040] Embodiment 2 is different from Embodiment 1 in that the driving member may also be a cylinder, which is more convenient.

[0041] The above is only an embodiment of the present invention, and the common knowledge such as the known specific structure and characteristics in the scheme is not described in detail here. It should be pointed out that for those skilled in the art, several deformations and improvements can be made without departing from the structure of the present invention, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. A method for preparing edible spirulina enzyme, characterized in that: The following steps are involved: S1: Use floating algae filtration method to place fresh spirulina in a 300-mesh filter and wash it with water. After washing the spirulina, filter it in a filter device until no water drips, forming algae mud; S2: comprising the following raw materials: 200-300g of algae mud, 500-800g of water, 30-50g of edible brown sugar, and 1-3g of bacterial stock solution; firstly dissolve the brown sugar completely into the water, then add the dry algae mud and bacterial stock solution into the sugar water, and put it into a barrel for sealed fermentation; S3: Starting from the second day after sealing, open the sealed plastic barrel every 24 hours to stir evenly and seal it in time. Continue to operate for 3 to 5 days. Then open the barrel to release air for 5 to 10 minutes every day. Continue to operate for 5 to 7 days. After the release is completed, stir once every 10 days, and continue to stir twice; S4: Ferment for 3 months at an air temperature of 15-30°C. If the temperature is below 15°C, extend the fermentation time. If the temperature exceeds 30°C, shade treatment is used. S5: Take samples for observation after 3 months of outdoor fermentation. If the enzyme is orange-red, emits a sour aroma, and has white colonies floating on the surface, transfer the enzyme to a ceramic jar for continued fermentation. The ceramic jar needs to be stored indoors away from light for at least 3 months.

2. The method for preparing a Spirulina edible enzyme according to claim 1, characterized in that: The water content of the algae mud is 87-92%.

3. The method for preparing a Spirulina edible enzyme according to claim 2, characterized in that: The filtering device comprises a filter screen with a mesh size of 300.

4. The filtering device for edible spirulina enzyme according to claim 3, characterized in that: The filtering device comprises a base, two symmetrical filtering elements for reciprocating scraping are arranged on the base, a scraping plate is arranged between the two filtering elements, and the filter screen is located below the scraping plate.

5. The filtering device for edible spirulina enzyme according to claim 4, characterized in that: The filter element comprises a fixed frame, a power member and a turbine are provided on the fixed frame, and the power member comprises a limit plate, a through slot is provided in the limit plate, a mounting block is horizontally slidably connected in the through slot, a scraper plate is fixedly connected between the two mounting blocks, the scraper plate contacts the filter screen, the mounting block is connected with a driving member that drives it to move horizontally, a displacement slot is provided on the mounting block, a limit block is rotatably connected in the mounting block, a worm is provided at the bottom of the limit block, the worm is meshed with the turbine, a positioning block is fixedly connected to the limit block, the mounting block is fixedly connected to the fixed block, a tension spring is provided between the fixed block and the positioning block, a supporting protrusion is provided in the displacement slot for limiting the limit block to move only in the direction where the positioning block is provided, driving gears are provided at both ends of the turbine, the driving gear is meshed with a driven gear, the driven gear is connected with a rotating shaft, the filter screen is wound around the rotating shaft, and the rotating shaft is rotatably connected to the fixed frame.

6. The filtering device for edible spirulina enzyme according to claim 5, characterized in that: The driving member comprises a screw rod, the mounting block is threadedly connected to the screw rod, the screw rod is connected to a stepping motor, and the stepping motor is fixedly connected to a fixing frame.

7. The filtering device for edible spirulina enzyme according to claim 5, characterized in that: The driving member is a cylinder.

8. A filtering device for edible spirulina enzyme according to claim 6 or 7, characterized in that: The fixing frame includes two symmetrical fixing plates, a connecting shaft is rotatably connected between the fixing plates, the turbine is fixedly connected to the connecting shaft, a plurality of positioning grooves are arranged on one side of the connecting shaft, a connecting block is fixedly connected to the fixing plate, the connecting block passes through and is slidably connected to the limiting shaft, one end of the limiting shaft is clamped in the positioning groove, a baffle is arranged on the other end of the limiting shaft, a limiting spring is arranged between the baffle and the connecting block, and the limiting spring is sleeved on the limiting shaft.

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