Lavender essential oil liposome prepared by taking silica gel as carrier as well as preparation method and application of lavender essential oil liposome

Abstract

The invention belongs to the technical field of flavors and fragrances, and particularly relates to a lavender essential oil liposome prepared by taking silica gel as a carrier as well as a preparation method and application of the lavender essential oil liposome. According to the invention, solid-phase particle silica gel and a wrapping material are fused together for the first time to prepare a no-load liposome, and the no-load liposome is coated with lavender essential oil to prepare a lavender essential oil liposome suspension. As a carrier, the silica gel has the advantages of small particle size, high and uniform specific surface area, mild action condition, high targeting property, no influence on the biological activity of the medicine and wide action range, and effectively solves the problems of complicated preparation process, large solvent dosage, low encapsulation efficiency, difficulty in large-scale preparation and the like of the existing liposome.

Description

Technical Field

[0001] The invention belongs to the technical field of flavors and fragrances, and particularly relates to lavender essential oil liposomes prepared with silica gel as a carrier, a preparation method and application thereof. Background Art

[0002] Lavender is a precious and important natural spice plant with a refreshing, rich and pleasant fragrance. The use of lavender to treat related diseases can be traced back to the ancient Roman and ancient Greek eras. It is mainly used in the clinical and cosmetic industries in the form of dry products or essential oils. Lavender essential oil is generally extracted from freshly picked lavender flower spikes through steam distillation. Lavender essential oil is now included in many national pharmacopoeias, including the European Pharmacopoeia, and is mainly used in aromatherapy or added to products as a pleasant fragrance and antibacterial agent. Lavender essential oil has a complex chemical composition and has multiple pharmacological effects such as bactericidal, antifungal, smooth muscle relaxation, sedative, antidepressant, etc., and is also effective for burns and insect bites. Linalool is one of its main components and plays an important role in the efficacy of lavender essential oil.

[0003] Liposomes are microvesicles, also known as lipid globules or liquid crystal microcapsules, which can encapsulate drugs in lipid bilayers. Nanoliposomes are not only used to deliver drugs, but also have broad application prospects in fields such as gene therapy and bioimaging. The appearance of liposomes is similar to micelles, but micelles are monolayer and are just a surfactant. Liposomes are bilayer structures composed of phospholipids and cholesterol. There are general liposomes and special performance liposomes. Common preparation methods for liposomes include ethanol injection method, thin film dispersion method, ultrasonic dispersion method, freeze drying method, reverse evaporation method, etc. However, in the prior art, the preparation process of liposomes has problems such as complicated preparation process, high production cost, large amount of organic solvent, and difficulty in large-scale preparation. Summary of the invention

[0004] In view of the above problems, the present invention firstly fuses solid phase particle silica gel with encapsulating material lecithin to prepare empty liposomes, and encapsulates medicinal lavender essential oil to prepare lavender essential oil liposomes. Silica gel as a carrier has the advantages of small particle size, high and uniform specific surface area, mild action conditions, high targeting, no effect on the biological activity of the drug, and wide action range, which effectively solves the problems of complicated existing liposome preparation process, large solvent dosage, low encapsulation rate, and difficulty in large-scale preparation.

[0005] In order to overcome the above-mentioned defects in the prior art, the present invention provides a method for preparing lavender essential oil liposomes, comprising the following steps:

[0006] (a) adding a wrapping material into anhydrous ethanol to prepare a wrapping material solution;

[0007] (b) immersing the solid phase particles in a coating material solution, removing the anhydrous ethanol, and combining the solid phase particles with the coating material to obtain empty liposomes;

[0008] (c) mixing the empty liposomes with the lavender essential oil solution to obtain a lavender essential oil liposome suspension.

[0009] The encapsulating material is one of lecithin and lecithin-cholesterol;

[0010] The mass ratio of lecithin to cholesterol is (4-8):1, preferably 6:1;

[0011] The solid phase particles are silica gel. The mechanism of action of the solid phase particles is to utilize the excellent specific surface area of ​​silica gel particles to make the coating material solution volatilize on the surface of silica gel particles to form a film;

[0012] The average particle size of the silica gel solid phase particles is 48 to 75 μm;

[0013] Preferably, in step (b), the anhydrous ethanol is removed by evaporation in a high-temperature water bath, freeze drying, or standing at room temperature;

[0014] Preferably, in step (c), the empty liposomes and the lavender essential oil solution are mixed in a manner comprising: vortexing, shaking, and ultrasound;

[0015] The mass concentration of the coating material in the coating solution is 0.02363 to 0.2 g / mL; preferably 0.03544 to 0.2 g / mL;

[0016] The mass ratio of the silica gel solid phase particles to the encapsulating material is (1.56-2.50):1; preferably (1.56-1.79):1;

[0017] The mass ratio of the lavender essential oil to lecithin is (6.2-8.4):100; preferably (6.2-6.7):100.

[0018] The solvent of the lavender essential oil solution is water, and the volume fraction of the lavender essential oil solution is 0.025% to 0.1%;

[0019] The present invention also provides a lavender essential oil liposome prepared by the above-mentioned preparation method, wherein the lavender essential oil liposome has a 24h permeability of not less than 40% in a transdermal diffusion experiment;

[0020] The encapsulation rate of the lavender essential oil liposome is 48.5-99.9%;

[0021] Furthermore, the encapsulation efficiency of the lavender essential oil liposomes was measured by HPLC-centrifugation method;

[0022] The centrifugal speed is 6000-12000 rpm, and the centrifugal time is 10-20 min.

[0023] The present invention also provides an application of the lavender essential oil liposomes prepared by the above-mentioned preparation method in preparing skin care products for removing spots and whitening, resisting bacteria and inflammation, relieving pain and itching, and regulating skin oil secretion and balancing.

[0024] The dosage form of the lavender essential oil liposome is a semisolid preparation, preferably an ointment.

[0025] Beneficial Effects

[0026] 1. The present invention uses silica gel solid phase particles as carriers for the first time, combines them with encapsulating materials to prepare empty liposomes, and then adds lavender essential oil to prepare lavender essential oil liposome suspension. The silica gel solid phase particles used in the present invention have a large specific surface area, so it is easier to form a thin and uniform film on the surface.

[0027] 2. The present invention adopts high-density silica gel solid phase particles as carriers. During the drying process, only a short-time, low-speed centrifugal operation is required to completely separate the lavender essential oil liposomes from the dispersed system, which greatly reduces the risk of instability of the lavender essential oil liposomes.

[0028] 3. The present invention adds an aqueous solution of lavender essential oil to empty liposomes to prepare lavender essential oil liposomes, without dissolving the lavender essential oil in an organic solvent, thereby avoiding the influence of heating on the lavender essential oil when removing the organic solvent.

[0029] 4. The present invention uses silica gel solid phase particles with a large specific surface area as a carrier, which greatly improves the utilization rate of the packaging material, does not require a large amount of packaging material, and further does not require a large amount of organic solvent for dissolution, thereby reducing costs and shortening preparation time. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 HPLC spectrum of the lavender essential oil liposome suspension of Example 1-3;

[0031] Figure 2 is the HPLC spectrum of the lavender essential oil liposome suspension of Example 4-5;

[0032] Figure 3 This is a microscope image (40X) of the silica gel aqueous dispersion in Example 1;

[0033] Figure 4 is a microscopic image (40X) of the empty liposome aqueous dispersion in Example 1;

[0034] Figure 5This is a microscope image (40X) of the lavender essential oil liposome suspension in Example 1. DETAILED DESCRIPTION

[0035] The present invention will be further described in detail below through specific implementation examples, but it should not be understood that the present invention is limited to the following examples, and all technologies realized based on the above content of the present invention belong to the scope of the present invention.

[0036] Lecithin was purchased from Beijing Aoboxing Biological Co., Ltd.; lavender essential oil was provided by Shandong Freda Biological Co., Ltd.; anhydrous ethanol was purchased from Tianjin Fuyu Fine Chemical Co., Ltd.; silica gel was manufactured by Qingdao Shibei District Haihua Desiccant Factory, model HG / T2354-92; artificial skin was Strat-MTM Membrane purchased from Merck Millipore Ltd; the remaining raw materials were commercially available unless otherwise specified.

[0037] Embodiment 1:

[0038] Weigh lecithin and add anhydrous ethanol solution to prepare 0.2g / mL lecithin solution, ultrasonicate for 10 minutes, and store under light-proof conditions. Weigh silica gel (average particle size of 62.5μm, particle size between 48-75μm) according to the mass ratio of silica gel to lecithin 2.50:1 and add it to the lecithin solution, stir while adding, evaporate in a 50°C water bath to obtain empty liposomes. Take the empty liposomes, add a lavender essential oil solution with a volume fraction of 0.025% according to the mass ratio of lavender essential oil to lecithin 8.4:100, shake for 10 minutes, and obtain a lavender essential oil liposome suspension.

[0039] Microscope images (40X) of silica gel aqueous dispersion, empty liposome aqueous dispersion and lavender essential oil liposome suspension are shown in Figure 3 to Figure 5 The concentration of silica gel in the silica gel aqueous dispersion was 14.53 g / mL; the concentration of empty liposomes in the empty liposome aqueous dispersion was 24.22 g / mL.

[0040] Embodiment 2:

[0041] Weigh lecithin and add anhydrous ethanol solution to prepare 0.2g / mL lecithin solution, ultrasonicate for 10 minutes, and store under light-proof conditions. Weigh silica gel (average particle size of 62.5μm, particle size between 48-75μm) according to the mass ratio of silica gel to lecithin 1.56:1 and add it to the lecithin solution, stir while adding, evaporate in a 50°C water bath to obtain empty liposomes. Take empty liposomes, add lavender essential oil solution with a volume fraction of 0.025% according to the mass ratio of lavender essential oil to lecithin 6.2:100, shake for 10 minutes, and obtain a lavender essential oil liposome suspension.

[0042] Embodiment 3:

[0043] Weigh lecithin and add anhydrous ethanol solution to prepare 0.2g / mL lecithin solution, ultrasonicate for 10 minutes, and store under light-proof conditions. Weigh silica gel (average particle size of 62.5μm, particle size between 48-75μm) according to the mass ratio of silica gel to lecithin 1.79:1 and add it to the lecithin solution, stir while adding, evaporate in a 50°C water bath to obtain empty liposomes. Take the empty liposomes, add a lavender essential oil solution with a volume fraction of 0.1% according to the mass ratio of lavender essential oil to lecithin 6.7:100, shake for 10 minutes, and obtain a lavender essential oil liposome suspension.

[0044] Embodiment 4:

[0045] The preparation conditions of this example are the same as those of Example 2, except that the lecithin in Example 2 is replaced by lecithin-cholesterol.

[0046] The mass ratio of lecithin to cholesterol is 6:1.

[0047] Embodiment 5:

[0048] The preparation conditions of this embodiment are the same as those of embodiment 3, except that the volume fraction of the lavender essential oil is 0.025%.

[0049] Comparative Example 1:

[0050] The preparation conditions of this comparative example are the same as those of Example 2, except that no silica gel solid phase particles are added.

[0051] Weigh lecithin and add anhydrous ethanol solution to prepare 0.2 g / mL lecithin solution, ultrasonicate for 10 minutes, and store in dark conditions. Put the lecithin solution in a rotary evaporator, evaporate at 50° C., add 0.025% lavender essential oil solution by volume according to the mass ratio of lavender essential oil to lecithin of 6.2:100, and ultrasonicate for 10 minutes to obtain a lavender essential oil liposome suspension.

[0052] Comparative Example 2:

[0053] Weigh lecithin and lavender essential oil, add them to an anhydrous ethanol solution, and ultrasonicate for 10 min to obtain a lavender essential oil organic solution, wherein the mass ratio of lavender essential oil to lecithin is 6.2:100, and the concentration of lecithin in the lavender essential oil organic solution is 0.2 g / mL.

[0054] The lavender essential oil organic solution was placed in an evaporating dish, and silica gel was added in a mass ratio of silica gel to lecithin of 1.56:1 under the condition of heating in a water bath at 50°C, stirring was performed while adding, and the solution was evaporated in a water bath, and then deionized water was added to obtain a lavender essential oil liposome suspension (wherein the volume ratio of deionized water to lavender essential oil was 1000:0.25).

[0055] Comparative Example 3:

[0056] Weigh lecithin and add anhydrous ethanol solution to prepare 0.2g / mL lecithin solution, ultrasonicate for 10 minutes, and store in dark. Take silica gel and place it in a rotary evaporator, then spray the lecithin solution into the rotary evaporator according to the mass ratio of silica gel to lecithin 1.56:1, the rotary evaporator temperature is 50°C, the vacuum degree is -0.098MPa, and empty liposomes are obtained. Take empty liposomes, add lavender essential oil aqueous solution with a volume fraction of 0.025% according to the mass ratio of lavender essential oil to lecithin of 6.2:100, shake for 10 minutes, and obtain lavender essential oil liposome emulsion.

[0057] Comparative Example 4:

[0058] The preparation conditions of this comparative example are the same as those of Example 3, except that the silica gel solid phase particles are replaced by montmorillonite of the same particle size.

[0059] Comparative Example 5:

[0060] The preparation conditions of this comparative example are the same as those of Example 3, except that the silica gel solid phase particles are replaced with TiO2 of the same particle size.

[0061] Test example 1: Encapsulation efficiency test

[0062] The detection method of the liposome encapsulation rate of lavender essential oil is as follows: after centrifuging 0.1 mL of lavender essential oil liposome suspension at 8000 rpm for 10 min, the supernatant is taken out and microfiltered through a 0.45 μm filter membrane to obtain a test solution for free essential oil detection (defined as a permeate). Anhydrous ethanol is added to the centrifugal sediment, ultrasonically dissolved, centrifuged at 8000 rpm for 10 min, the supernatant is taken out, and microfiltered through a 0.45 μm filter membrane to obtain a test solution for encapsulated essential oil detection (defined as a concentrate). Liquid chromatography analysis is performed to detect the amount of linalool in the free essential oil and the test solution of the encapsulated essential oil, and the encapsulation rate is calculated according to the following formula.

[0063] Encapsulation rate (100%) = amount of essential oil encapsulated / (amount of free essential oil + amount of essential oil encapsulated) × 100%

[0064] Among them, since silica gel solid phase particles are not used as carriers in Comparative Example 1, the density of lavender essential oil liposomes is relatively small, and it is impossible to obtain permeate and concentrate by the above-mentioned centrifugal method. Therefore, the detection method of Comparative Example 1 needs to be replaced as follows: 0.1 mL of lavender essential oil emulsion is placed in a centrifuge tube (CF), centrifuged at 8000 rpm for 10 min, the supernatant is taken out and added to an ultrafiltration centrifuge tube (UF-CF, the ultrafiltration membrane pore size is 30 KD), 0.3 mL of pure water is added, and the centrifugation time at 8000 rpm is 10 min, and the permeate solution is taken as the test solution for free essential oil detection. Continue to add 0.4 mL of anhydrous ethanol to the above-mentioned CF, weigh, ultrasonically dissolve, add to the above-mentioned UF-CF, ultrafiltration centrifuge at 8000 rpm for 10 min, take the permeate solution to make up the weight lost with anhydrous ethanol, as the test solution for encapsulated essential oil detection.

[0065] HPLC conditions: bonded silica gel C 18 (4.6mm*250mm, 5μm), with acetonitrile (A)-0.2% phosphoric acid aqueous solution (B) as the mobile phase, gradient elution (0-30min, A% (35%~80%); 31-40min, A% 100%), flow rate 1.0mL·min, column temperature 30℃, detection wavelength: 203nm, injection volume: 10μL.

[0066] Table 1 Encapsulation efficiency of lavender essential oil liposomes (%)

[0067]

[0068]

[0069] As shown in Table 1, the encapsulation efficiency of the lavender essential oil liposomes prepared in each embodiment and comparative example is as follows:

[0070] Compared with Example 2, Example 1 has a lower encapsulation rate. This is because lecithin is the raw material for forming the liposome shell. Within a certain dosage range of lecithin and other conditions remain unchanged, the amount of lecithin is small, the number of liposomes formed is small, and the encapsulation rate is poor; compared with Example 5, Example 2 has a lower encapsulation rate. This is because the amount of lecithin is large, which will cause the lecithins to adhere to each other and cannot form a spherical shape with a good shape, resulting in a small drug holding capacity or a large liposome shell thickness, making it difficult for a small amount of drug to enter.

[0071] Compared with Example 3, the encapsulation efficiency of Example 5 is significantly improved, indicating that the concentration of lavender essential oil has a great influence on the encapsulation efficiency.

[0072] Figure 3 to Figure 5The results showed that there were obvious differences between the empty liposomes and lavender essential oil liposomes in the aqueous dispersion and the surface of the silica gel particles. Dense particles were attached to the surface of the empty liposomes and lavender essential oil liposome particles, and there was no obvious difference in the size and shape of the particles. The surface of the silica gel particles was smooth, indicating that liposome particles were formed on the silica gel surface after the encapsulating material was hydrated.

[0073] Compared with the prior art, the present application has significant advantages in that a higher embedding rate can be obtained within a wider parameter range, and the preparation process is simple, the amount of organic solvent used is small, the preparation time is short, and large-scale preparation is possible.

[0074] According to Table 1, the encapsulation efficiency of Comparative Example 1 is much lower than that of Example 2. This is because in the process of preparing lavender essential oil liposomes by thin film dispersion method, the thickness of the film formed by the encapsulating material on the wall of the rotary evaporator bottle is uneven, resulting in a low encapsulation efficiency of the lavender essential oil liposomes.

[0075] According to Table 1, the encapsulation efficiency of Comparative Example 2 is much lower than that of Example 2. This is because during the evaporation of the solvent of the lavender essential oil and lecithin solution on the surface of the solid particles, the volatile lavender essential oil component also evaporates and causes loss, which also affects the encapsulation effect.

[0076] According to Table 1, the encapsulation rate of Comparative Example 3 is much smaller than that of Example 2. This is because during the spraying process of the membrane material solution, since the system is in a vacuum state, it is difficult for the membrane material solution to be effectively sprayed onto the surface of the solid phase particles to form a film. More tiny spots are formed on the surface of the solid phase particles, and some membrane material particles are formed. Therefore, for the same amount of membrane material solution, the film-forming area of ​​Comparative Example 3 is much smaller than that of Example 2, and therefore the encapsulation rate of Comparative Example 3 is much smaller than that of Example 2.

[0077] In Comparative Examples 4 and 5, due to the rough surface of montmorillonite and its strong adsorption capacity, the encapsulating material agglomerates on the surface of montmorillonite and cannot form a well-shaped sphere; since the surface of TiO2 is too smooth, the surface of the carrier is difficult to wet, resulting in poor adsorption of the TiO2 carrier on the encapsulating material, making it difficult to form a film on its surface.

[0078] like Figure 1 and Figure 2 The HPLC spectra of the lavender essential oil liposome free essential oil test solution (permeate) and the encapsulated essential oil test solution (concentrate) prepared in Examples 1 to 5 are shown. It can be seen from the figure that both the permeate and the concentrate have peaks at the linalool chromatographic peak (chromatographic peak retention time is about 15 min), but the peak of the permeate is very small, while the concentrate has a strong peak, indicating that the preparation method provided by the present invention can effectively encapsulate lavender essential oil.

[0079] Test Example 2: Transdermal Diffusion Experiment

[0080] Utilize transdermal diffusion test device, add mass concentration in the diffusion cell and be 40% PEG400 solution, get each 0.3mL of the prepared lavender essential oil liposome suspension and lavender essential oil aqueous solution (volume fraction is 0.1%) of Example 3, add Tween-20 respectively and make it to reach 5% (mass fraction), be evenly dispersed on the smooth surface of artificial skin, see through at room temperature, take out 0.2mL sample solution (i.e. lavender liposome suspension sees through solution, lavender essential oil aqueous solution sees through solution) from the diffusion cell after an interval of 24h and carry out liquid chromatography determination, simultaneously with mass concentration be 40% PEG400 solution to make up volume, and high performance liquid chromatography calculates transmittance and sees Table 2.

[0081] Table 2 Penetration of lavender essential oil liposome suspension and lavender essential oil aqueous solution

[0082] Volume fraction (%) 24h transmittance (%) Lavender essential oil liposomal suspension 0.1 41.6 Lavender essential oil in water 0.1 18.35

[0083] Test Example 3: Acetylcholinesterase Inhibitory Activity

[0084] 1. Solution preparation

[0085] Phosphate buffered saline (PBS): Take 0.68 g of potassium dihydrogen phosphate, add 29.1 mL of 0.1 mol / L sodium hydroxide solution, and dilute with water to 100 mL.

[0086] Lavender essential oil sample solution: Take lavender essential oil and dissolve it in a 10 mg / ml Tween 20 aqueous solution to prepare a lavender essential oil sample solution with a volume fraction of 0.025%.

[0087] DTNB solution (0.01 M): Dissolve 3.9 mg DTNB in ​​1 mL DMSO and store at 4°C in the dark.

[0088] ATCI solution: 108.35 mg dissolved in 4 mL of water and stored at 4°C.

[0089] AChE solution: 3 mg dissolved in 3.5 mL PBS and stored at 4°C.

[0090] Huperzine A solution: 2 mg dissolved in 1 mL of water, diluted to 0.01 mg / mL.

[0091] 2 Experimental steps

[0092] The experiment set up a blank reference group (BR), a blank control group (BC), a sample reference group (SR), a sample group (S), and a positive control group (C).

[0093] Group S: 5 μL of sample solution, 170 μL of phosphate buffer (0.1 mol / L, pH 7.0), 15 μL of AChE solution and 10 μL of DTNB solution were added to the wells in sequence, shaken thoroughly, and placed at 4°C for 20 minutes. After 20 minutes, 10 μL of ATCI solution was added dropwise and allowed to react in a 37°C water bath for 20 minutes. After the reaction, the absorbance A was measured in an ELISA reader. S .

[0094] The sample solutions are: lavender essential oil sample solution, lavender essential oil liposome suspension permeate solution prepared in Detection Example 3, and lavender essential oil aqueous solution permeate solution prepared in Detection Example 3.

[0095] SR group: 15 μL of PBS solution replaced 15 μL of AChE solution. Other steps were the same as those in S group. The absorbance A was measured. SR .

[0096] BR group: 175 μL of PBS solution replaced 5 μL of sample solution and 170 μL of phosphate buffer. Other steps were the same as the sample group. The absorbance A was measured. BR .

[0097] BC group: 20 μL of dimethyl sulfoxide (DMSO) and 140 μL of PBS solution replaced 5 μL of sample solution and 170 μL of phosphate buffer. Other steps were the same as the sample group. The absorbance A was measured. BC .

[0098] Group C: The lavender essential oil sample solution was replaced with 5 μL of Huperzine A solution, and the other steps remained unchanged. The absorbance A was measured. C .

[0099] The inhibition rate of nuciferine samples was calculated using the formula:

[0100] The inhibition rate of Huperzine A solution was calculated using the formula:

[0101] Table 3 Lavender essential oil acetylcholinesterase inhibition rate

[0102]

[0103]

[0104] As shown in Table 3, the inhibition rates of acetylcholinesterase of lavender essential oil sample solution and Huperzine A reference substance, and the inhibition rates of acetylcholinesterase of lavender essential oil liposome suspension permeation solution and lavender essential oil aqueous solution permeation solution in Test Example 3 are shown. It can be concluded from Table 3 that the lavender oil sample and the lavender essential oil liposome suspension permeation solution in Test Example 3 have obvious acetylcholinesterase inhibition, indicating that lavender oil has a strong transdermal ability after being prepared into liposomes, which confirms the reliability of the permeability data. Although the lavender essential oil aqueous solution in Test Example 3 also has a certain permeability, it is significantly lower than the permeability of lavender oil liposomes, so no acetylcholinesterase inhibition activity is detected in its permeation solution.

Claims

1. A method for preparing lavender essential oil liposomes, characterized in that: The steps include: (a) adding a wrapping material into anhydrous ethanol to prepare a wrapping material solution; (b) immersing the solid phase particles in a coating material solution, removing the anhydrous ethanol, and combining the solid phase particles with the coating material to obtain empty liposomes; (c) mixing the empty liposome with the lavender essential oil solution to obtain a lavender essential oil liposome suspension; The solid phase particles are silica gel.

2. The method for preparing lavender essential oil liposome according to claim 1, characterized in that: The packaging material is one of lecithin and lecithin-cholesterol.

3. The method for preparing lavender essential oil liposome according to claim 2, characterized in that: The mass ratio of lecithin to cholesterol is (4-8):1, preferably 6:

1.

4. The method for preparing lavender essential oil liposome according to claim 1, characterized in that: The average particle size of the silica gel solid phase particles is 48 to 75 μm.

5. The method for preparing lavender essential oil liposome according to claim 1, characterized in that: The mass concentration of the coating material in the coating solution is 0.02363 to 0.2 g / mL, preferably 0.03544 to 0.2 g / mL; The solvent of the lavender essential oil solution is water. Preferably, the volume fraction of the lavender essential oil solution is 0.025% to 0.1%.

6. The method for preparing lavender essential oil liposome according to claim 1, characterized in that: The mass ratio of the silica gel solid phase particles to the encapsulating material is (1.56-2.50):1; preferably (1.56-1.79):

1.

7. The method for preparing lavender essential oil liposome according to claim 2, characterized in that: The mass ratio of the lavender essential oil to lecithin is (6.2-8.4):100; preferably (6.2-6.7):

100.

8. A lavender essential oil liposome prepared by the preparation method according to any one of claims 1 to 7, characterized in that: The 24h permeability of the lavender essential oil liposome is not less than 40%; The encapsulation rate of the lavender essential oil liposome is 48.5-99.9%.

9. Use of lavender essential oil liposomes prepared by the preparation method of any one of claims 1 to 7 or the lavender essential oil liposomes described in claim 8 in preparing skin care products for removing spots and whitening, antibacterial and anti-inflammatory, relieving pain and itching, and regulating skin oil secretion and balancing.

10. The use of the lavender essential oil liposome according to claim 9 in preparing a skin care product for removing spots and whitening, antibacterial and anti-inflammatory, relieving pain and itching, and regulating skin oil secretion and balancing, characterized in that: The dosage form of the lavender essential oil liposome is a semisolid preparation, preferably an ointment.

Images