An oil-controlling and acne-removing composition

Abstract

This invention discloses an oil-controlling and acne-reducing composition, belonging to the field of cosmetics. The composition comprises Pueraria lobata extract, Artemisia annua extract, sarcosine, and Asparagus officinalis extract. The composition provided by this invention achieves excellent oil-controlling and acne-reducing effects through the synergistic effect of the combined use of these ingredients.

Description

Technical Field

[0001] This application relates to the field of cosmetics, specifically to an oil-controlling and acne-removing composition. Background Technology

[0002] Acne is a common skin problem, mainly caused by excessive sebum secretion, abnormal follicular keratinization, Propionibacterium acnes infection, and inflammatory reactions. Although there are many types of acne treatment products available, they often have problems such as strong irritation, insignificant effects, or dependence on hormones, making it difficult to meet consumers' needs for gentle and effective acne treatment products.

[0003] In recent years, natural plant extracts have gradually become important ingredients in acne-fighting cosmetics due to their gentleness, safety, and diverse bioactivities. For example, green tea extract, white willow bark extract, and phellodendron bark extract are widely used in acne products, exhibiting significant antibacterial, anti-inflammatory, and oil-controlling effects. Furthermore, some plant extracts can regulate the skin's microecological balance and reduce acne recurrence.

[0004] The formulation design of acne-fighting cosmetics should follow these principles: fast onset of action and high effectiveness; avoid the use of prohibited ingredients such as antibiotics and hormones, and maximize the use of natural ingredients; at the same time, focus on repairing the skin and improving its immunity. The formulation design approach includes removing surface dead skin cells, unblocking sebum secretion pathways, adding oil-controlling ingredients, inhibiting excessive sebum secretion, and providing anti-inflammatory and antioxidant effects.

[0005] Although various plant extracts have been used in acne treatment products, existing products still have certain limitations. For example, some products are not effective enough in treating acne, or are not gentle enough for sensitive skin. Therefore, there is a significant market demand for developing a natural plant composition that can effectively treat acne while gently caring for the skin. Summary of the Invention

[0006] The purpose of this invention is to provide an oil-controlling and acne-removing composition, which includes Pueraria lobata extract, Artemisia annua extract, sarcosine, and Asparagus officinalis extract, and has high safety and excellent oil-controlling and acne-removing effects.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] In a first aspect, the present invention provides an oil-controlling and acne-removing composition comprising the following raw materials: Pueraria lobata extract, Artemisia annua extract, sarcosine, and Asparagus officinalis extract.

[0009] Preferably, the oil-controlling and acne-removing composition comprises the following raw materials in parts by weight:

[0010] Pueraria lobata extract: 0.5-2 parts;

[0011] Artemisia annua extract: 0.1-2 parts;

[0012] Creatine: 1-10 parts;

[0013] Asparagus spicata extract: 0.2-5 parts.

[0014] Furthermore, the oil-controlling and acne-removing composition comprises the following raw materials in parts by weight:

[0015] Pueraria lobata extract: 1.3-2 parts;

[0016] Artemisia annua extract: 1.2-2 parts;

[0017] Creatine: 5-10 parts;

[0018] Asparagus spicata extract: 2.5-5 parts.

[0019] Most preferably, the oil-controlling and acne-removing composition comprises the following raw materials in parts by weight:

[0020] Pueraria lobata extract: 1 part;

[0021] Artemisia annua extract: 1 part;

[0022] Creatine: 5 parts;

[0023] Asparagus spicata extract: 2.7 parts.

[0024] Secondly, the present invention provides a serum, the serum comprising the following components by weight percentage:

[0025] The oil-controlling and acne-removing composition described in the first aspect: 1-10 wt%;

[0026] Emulsifier: 1-5 wt%;

[0027] Moisturizer: 10-12 wt%;

[0028] Emollient: 1-5 wt%;

[0029] Add deionized water to bring the total to 100 wt%.

[0030] Preferably, the emulsifier is one or more of polysorbate-20, polysorbate-80, PEG-40 hydrogenated castor oil, and lecithin; the moisturizer is one or more of glycerin, hyaluronic acid, propylene glycol, butylene glycol, and panthenol; and the emollient is one or more of squalane, olive oil, polydimethylsiloxane, and grape seed oil.

[0031] Thirdly, the present invention provides a method for preparing an essence, the specific steps of which are as follows:

[0032] S1: Mix 1 / 3 of the deionized water with the humectant until homogeneous to obtain mixture A;

[0033] S2: Mix 1 / 3 of the deionized water with the emulsifier and emollient to obtain mixture B;

[0034] S3: Mix 1 / 3 of the deionized water, the oil-controlling and acne-removing composition described in the first aspect, and mixture A evenly, and homogenize for 0.5-1 h to obtain mixture C;

[0035] S4: Mix mixture B and mixture C evenly, homogenize for 0.5-1 hour, fill, sterilize, seal, and store in the dark at room temperature.

[0036] Compared with existing technologies, the beneficial effects of this invention are as follows: This invention provides an oil-controlling and acne-removing composition, which is formulated from Pueraria lobata extract, Artemisia annua extract, sarcosine, and Asparagus officinalis extract. Pueraria lobata extract has anti-inflammatory, antioxidant, and skin metabolism-regulating effects. It can reduce acne and inflammation around pimples by inhibiting the production of inflammatory factors, and can also promote skin metabolism and help repair damaged skin barriers. Artemisia annua extract can reduce sebum accumulation on the skin surface by inhibiting sebum secretion from sebaceous gland cells, and also has a certain antibacterial effect, inhibiting the proliferation of Propionibacterium acnes. Sarcosine can reduce excessive sebum secretion. Asparagus officinalis can reduce skin inflammation, reduce redness and swelling around acne, regulate the skin microenvironment, and reduce sebum secretion. Among the above-mentioned ingredients, Pueraria lobata extract and Asparagus officinalis extract reduce sebum secretion by regulating skin metabolism and water-oil balance, while Artemisia annua extract can directly inhibit sebum secretion from sebaceous gland cells, further synergistically controlling oil production; Artemisia annua extract and Asparagus officinalis extract play a synergistic role in antibacterial and anti-inflammatory effects by inhibiting the growth of Propionibacterium acnes and reducing the production of inflammatory factors, thereby reducing potential inflammatory responses.

[0037] The research results show that the combined use of Pueraria lobata extract, Artemisia annua extract, sarcosine, and Asparagus officinalis extract has a significant synergistic effect and can significantly control oil and remove acne. Detailed Implementation

[0038] To better understand the present invention, the present invention will be further described below with reference to specific application examples. The terminology used in the application examples is for describing specific specific implementations and does not constitute a limitation on the scope of protection of the present invention.

[0039] Unless otherwise specified, experimental methods in the following examples are generally performed under standard conditions or as recommended by the manufacturer. Unless otherwise stated, calculations are based on percentages and parts by mass.

[0040] Some of the raw materials and their sources are as follows:

[0041] Pueraria lobata extract: purchased from Xi'an Youshuo Biotechnology Co., Ltd. and Beijing Dongfang Miaosen Biotechnology Co., Ltd.

[0042] Artemisia annua extract: purchased from Yunnan Yingge Biotechnology Co., Ltd.

[0043] Creatine: Purchased from Jiahe Biotechnology Co., Ltd.

[0044] Seaweed extract: purchased from Guangzhou Baihaobo Co., Ltd.

[0045] All other raw materials and reagents are commercially available.

[0046] The specific mass proportions of each ingredient in the oil-controlling and acne-reducing composition are as follows:

[0047] Composition 1:

[0048] Wild kudzu root extract: 1 part, Artemisia annua extract: 1 part, sarcosine: 5 parts, Asparagus officinalis extract: 2.7 parts.

[0049] Composition 2:

[0050] Wild kudzu root extract: 0.5 parts, Artemisia annua extract: 2 parts, sarcosine: 1 part, Asparagus officinalis extract: 5 parts.

[0051] Composition 3:

[0052] Wild kudzu root extract: 2 parts, Artemisia annua extract: 0.1 parts, sarcosine: 10 parts, Asparagus officinalis extract: 0.2 parts.

[0053] Composition ①:

[0054] Unlike composition 1, it lacks Pueraria lobata extract, and the missing mass is made up by Artemisia annua extract, sarcosine, and Asparagus officinalis extract in a mass ratio of 1:5:2.7.

[0055] Composition ②:

[0056] Unlike composition 1, it lacks Artemisia annua extract, and the missing mass is made up by Pueraria lobata extract, sarcosine, and Asparagus officinalis extract in a mass ratio of 1:5:2.7.

[0057] Composition ③:

[0058] Unlike composition 1, it lacks creatine, and the missing mass is made up by extracts of Pueraria lobata, Artemisia annua, and Asparagus cochinchinensis in a mass ratio of 1:1:2.7.

[0059] Composition ④:

[0060] Unlike composition 1, it lacks Asparagus officinalis extract, and the missing parts are made up by the mass ratio of Pueraria lobata extract, Artemisia annua extract and sarcosine in a ratio of 1:1:5.

[0061] Composition ⑤:

[0062] Wild kudzu root extract: 5 parts, Artemisia annua extract: 3.8 parts, sarcosine: 0.8 parts, and Asparagus officinalis extract: 0.1 parts.

[0063] The mass percentages of each component in the serum are as follows:

[0064] Table 1. Serum components and their mass percentage

[0065] Components Application Example 1 Application Example 2 Application Example 3 Application Example 4 Application Example 5 Blank application example Oil-controlling and acne-fighting composition / wt% 5 5 5 1 10 - Composition serial number Composition 1 Composition 2 Composition 3 Composition 1 Composition 1 - Emulsifier / wt% 3 3 3 1 5 3 Moisturizer / wt% 11 11 11 12 10 11 Emollients / wt% 3 3 3 1 5 3 Deionized water / wt% Add to 100 Add to 100 Add to 100 Add to 100 Add to 100 Add to 100

[0066] The emulsifier is polysorbate-20, polysorbate-80, and PEG-40 hydrogenated castor oil, and the mass ratio of polysorbate-20, polysorbate-80, and PEG-40 hydrogenated castor oil is 1:0.3:0.4.

[0067] The moisturizer is glycerin and panthenol, and the mass ratio of glycerin to panthenol is 1:0.5;

[0068] The moisturizer is olive oil and grape seed oil, and the mass ratio of olive oil to grape seed oil is 1:1.

[0069] The preparation methods for the above application examples 1-5 are as follows:

[0070] S1: Mix 1 / 3 of the deionized water with the humectant until homogeneous to obtain mixture A;

[0071] S2: Mix 1 / 3 of the deionized water with the emulsifier and emollient to obtain mixture B;

[0072] S3: Mix 1 / 3 of the deionized water, the oil-controlling and acne-removing composition and mixture A evenly, and homogenize for 1 hour to obtain mixture C;

[0073] S4: Mix mixture B and mixture C evenly, homogenize for 1 hour, fill, sterilize, seal, and store in the dark at room temperature.

[0074] The method for preparing blank application examples is as follows:

[0075] S1: Mix 1 / 3 of the deionized water with the humectant until homogeneous to obtain mixture A;

[0076] S2: Mix 1 / 3 of the deionized water with the emulsifier and emollient to obtain mixture B;

[0077] S3: Mix 1 / 3 of the deionized water and mixture A evenly, and homogenize for 1 hour to obtain mixture C;

[0078] S4: Mix mixture B and mixture C evenly, homogenize for 1 hour, fill, sterilize, seal, and store in the dark at room temperature.

[0079] Experiment 1: Patch Test

[0080] The present invention experimentally verifies the biosafety of application examples 1-5 and blank application examples.

[0081] The irritation of cosmetics was evaluated using the 2015 Cosmetic Safety Technical Specifications as a reference standard. The test method was a skin patch test, and 60 people aged 16-65 years were randomly distributed to participate in the test.

[0082] Test method: Place the test substance into a patch applicator at a dosage of 0.020-0.025 g. Cover the patch applicator containing the test substance onto the back or flexor side of the forearm of the subject with non-irritating adhesive tape. Gently press with the palm of your hand to ensure even adhesion to the skin surface. Leave on for 24 hours. Remove the patch applicator and observe the skin reaction 30 minutes after the pressure mark disappears. If the result is negative, observe again at 24 hours and 48 hours after the patch test.

[0083] Evaluation criteria:

[0084] Grade 0: Negative reaction;

[0085] Grade 1: Suspicious reaction, with only slight erythema;

[0086] Grade 2: Weak positive reaction, erythema, infiltration, edema, and possible papules;

[0087] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules may be present, and the reaction may extend beyond the test area;

[0088] Grade 4: Extremely positive reaction, with obvious erythema, severe infiltration, edema, confluent herpes, and reaction extending beyond the test area.

[0089] Test results: All subjects had negative skin reactions.

[0090] Experiment 2: Evaluation of oil control efficacy

[0091] Test samples: Compositions 1-3, Compositions ①-⑤.

[0092] Sample preparation: Each sample was prepared into a 5wt% solution using Tris-HCl (pH=7.0) buffer.

[0093] Experimental Principle: 5α-Reductase is a membrane protease located on microsomes and the cell nucleus. Using the reduced coenzyme NADPH as a hydrogen donor, it catalyzes the conversion of testosterone to dihydrotestosterone (DHT), which induces excessive sebum secretion from the sebaceous glands. By inhibiting 5α-reductase activity, the production of DHT is reduced, thereby lowering the level of sebum secretion from the sebaceous glands. Using 5α-reductase inhibition activity as an indicator, this experimental method was used to explore the product's inhibitory effect on 5α-reductase, thus evaluating the product's oil-controlling efficacy.

[0094] Experimental Method: 1 mL of the reaction system contained 100 μmol / L NADPH, 5 μmol / L testosterone, 0.6 mg / L 5α-reductase, and 200 μL of prepared sample solution, with the remainder made up with Tris-HCl buffer. The blank group used Tris-HCl (pH=7.0) buffer instead of the prepared sample solution. The reaction system was incubated at 37℃ for 10 min. The absorbance of the sample group and the blank group at OD340 nm was measured at 0 min and 10 min, respectively. The inhibition rate of the experimental sample against 5α-reductase was calculated according to the following formula. The reaction was repeated three times and the average value was taken to evaluate the inhibitory effect of the experimental sample on 5α-reductase. The results are shown in Table 2. The experimental results are expressed as mean ± SD.

[0095] 5α-reductase inhibition rate = [(B0-B 10 )-(A0-A 10 )] / (B0-B 10 )×100%

[0096] In the above formula: A0 - absorbance of the sample group at 0 min of reaction;

[0097] A 10 -Absorbance of the sample group after 10 minutes of reaction;

[0098] B0 - Absorbance of the blank group at 0 min of reaction;

[0099] B 10 -Absorbance of the blank group after 10 minutes of reaction.

[0100] Experiment 3: Evaluation of Acne-Clearing Efficacy

[0101] Test samples: Compositions 1-3, Compositions ①-⑤.

[0102] Test sample preparation: Prepare a 5wt% sample solution using deionized sterile water.

[0103] Experimental method: Propionibacterium acnes strain was activated and then diluted to 1.0 x 10⁻⁶. 6CFU / mL, 100 μL of diluted bacterial suspension and 20 μL of sample solution were added to each well. The blank control group used an equal volume of deionized sterile water instead of the sample solution. The 96-well plate containing the sample solution and bacterial suspension was incubated in an anaerobic incubator for 48 h. The OD600 nm of each well was measured using a microplate reader. The bacterial content in each well was determined based on the absorbance, and the inhibition rate of *Propionibacterium acnes* was calculated using the formula below. The results are shown in Table 2. The experimental results are expressed as mean ± SD.

[0104] The culture medium required for the activation and culture of Propionibacterium acnes is Propionibacterium acnes liquid culture medium, which was purchased from Shifeng Biotechnology.

[0105] Inhibition rate % = (OD blank - OD sample) / OD blank

[0106] OD blank refers to the OD value of the blank group;

[0107] OD sample refers to the OD value of the sample group.

[0108] Table 2 Evaluation results of the oil-controlling and acne-removing effects of the composition

[0109] experimental group 5α-Reductase Inhibition Rate / % Inhibition rate of Propionibacterium acnes / % Composition 1 48.18±0.91 44.17±0.88 Composition 2 47.74±1.07 43.56±0.67 Composition 3 47.98±0.88 43.28±0.49 Composition ① 26.58±8.04* 36.34±0.77* Composition ② 30.73±8.35* 37.81±0.41* Composition ③ 23.51±3.10* 38.40±0.21* Composition ④ 32.04±4.36* 38.20±0.29* Composition ⑤ 43.31±2.48* 40.68±0.19*

[0110] Note: "*" indicates that p < 0.05 compared to composition 1.

[0111] As shown in Table 2, compositions 1-3 have significant oil-controlling and acne-removing effects. Comparing the results of composition 1 with those of compositions ①-④, it can be seen that there is a significant synergistic effect among the Pueraria lobata extract, Artemisia annua extract, sarcosine, and Asparagus officinalis extract as defined in this invention. Comparing the results of composition 1 with those of composition ⑤, it can be seen that when the proportions of Pueraria lobata extract, Artemisia annua extract, sarcosine, and Asparagus officinalis extract are within the range defined in this invention, the oil-controlling and acne-removing effects of this composition are more significant.

[0112] Experiment 4: Human body oil control efficacy test

[0113] Thirty volunteers with oily facial skin (aged 18-26) were recruited and divided into 6 groups of 5. Each group was assigned to apply the serums from Application Examples 1-5 and the blank application example, respectively. The serums were applied twice daily, morning and evening, after cleansing, using 2mL each time, for 30 days. The results are shown in Table 3.

[0114] 30-day oil price reduction rate = (measurement value on day 0 - measurement value on day 30) / measurement value on day 0 × 100%.

[0115] The measured values ​​are obtained by measuring a fixed area of ​​skin using a skin oil analyzer (SebumScale).

[0116] Table 3 Results of Human Body Oil Control Efficacy Test

[0117] experimental group 30-day fat loss rate (mean) / % Application Example 1 27.42 Application Example 2 26.57 Application Example 3 26.94 Application Example 4 12.63 Application Example 5 30.34 Blank application example 1.31

[0118] As shown in Table 3 above, Application Examples 1-5 have a significant oil control effect compared to the blank application examples.

[0119] The embodiments described above are some, but not all, of the embodiments of this application. The detailed description of the embodiments of this application is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

Claims

1. An oil-controlling and acne-removing composition, characterized in that, Includes the following quantities of raw materials: Pueraria lobata extract: 0.5-2 parts; Artemisia annua extract: 0.1-2 parts; Creatine: 1-10 parts; Asparagus spicata extract: 0.2-5 parts.

2. The composition according to claim 1, characterized in that, Includes the following quantities of raw materials: Pueraria lobata extract: 1.3-2 parts; Artemisia annua extract: 1.2-2 parts; Creatine: 5-10 parts; Asparagus spicata extract: 2.5-5 parts.

3. The composition according to claim 1, characterized in that, Includes the following quantities of raw materials: Pueraria lobata extract: 1 part; Artemisia annua extract: 1 part; Creatine: 5 parts; Asparagus spicata extract: 2.7 parts.

4. The use of the oil-controlling and acne-removing composition as described in any one of claims 1-3 in the preparation of skin care products having oil-controlling and / or acne-removing effects.

5. The application as described in claim 4, characterized in that, The skincare product can be any one of serum, lotion, or cream.

6. An essence, characterized in that, It contains the following components by weight percentage: The oil-controlling and acne-removing composition according to any one of claims 1-3: 1-10 wt% Emulsifier: 1-5 wt%; Moisturizer: 10-12 wt% Emollient: 1-5 wt%; Add deionized water to bring the total to 100 wt%.

7. The essence according to claim 6, characterized in that, The emulsifier is one or more of polysorbate-20, polysorbate-80, PEG-40 hydrogenated castor oil, and lecithin.

8. The essence according to claim 6, characterized in that, The moisturizer is one or more of glycerin, hyaluronic acid, propylene glycol, butylene glycol, and panthenol.

9. The essence according to claim 6, characterized in that, The emollient is one or more of squalane, olive oil, polydimethylsiloxane, and grape seed oil.

10. A method for preparing an essence as described in any one of claims 6-9, characterized in that, The preparation steps include the following: S1: Mix 1 / 3 of the deionized water with the humectant until homogeneous to obtain mixture A; S2: Mix 1 / 3 of the deionized water with the emulsifier and emollient to obtain mixture B; S3: Mix 1 / 3 of the deionized water, the oil-controlling and acne-removing composition and mixture A evenly, and homogenize for 0.5-1 h to obtain mixture C; S4: Mix mixture B and mixture C evenly, homogenize for 0.5-1 hour, fill, sterilize, seal, and store in the dark at room temperature.