Eriocheir sinensis origin identification method based on electronic tongue

Abstract

The invention relates to an Eriocheir sinensis origin identification method based on an electronic tongue. The method comprises the following steps: acquiring a preset number of Eriocheir sinensis from at least two preset origin; obtaining a crab meat sample based on a preset gram weight, and preparing a sample solution corresponding to each producing area; obtaining analysis solutions based on the preset capacity, and detecting the analysis solutions one by one by using the electronic tongue to obtain taste feature values of the analysis solutions corresponding to the preset taste features; based on the taste feature values corresponding to the analysis solutions, SPSS is used for discriminant analysis, and discriminant functions corresponding to the producing areas are constructed respectively; and identifying the eriocheir sinensis of an unknown producing area in combination with the discrimination function. The method has the characteristics of convenience, objectiveness and rapidness.

Description

Technical Field

[0001] The present invention relates to the technical field of aquatic product quality evaluation and origin tracing, and in particular to a method for identifying the origin of Chinese mitten crab based on an electronic tongue. Background Art

[0002] The origin protection of Chinese mitten crab mainly relies on the anti-counterfeiting system with coding as the carrier. However, in the actual production and application process, many subjective and objective factors such as artificial imitation of labels and easy loss of labels have caused great troubles to the origin protection. With the development of technology in recent years, methods based on geometric morphology, mineral elements and stable isotopes have been widely used in the origin traceability of aquatic products. However, the indicators detected by these methods are not the intuitive taste characteristics of Chinese mitten crabs from various origins. In addition, the sensory evaluation based on taste characteristics is simply subject to the influence of sensory evaluators and the detection time is long.

[0003] Therefore, in response to the above problems, it is necessary to establish a method for identifying the origin of Chinese mitten crab based on electronic tongue from the perspective of objective and accurate analysis and quantitative taste indicators to meet the needs of actual use. Summary of the invention

[0004] In order to overcome the deficiencies of the prior art, the present invention proposes a method for identifying the origin of Chinese mitten crab based on electronic tongue.

[0005] In order to achieve the above object, the present invention adopts the following technical solution:

[0006] A method for identifying the origin of Chinese mitten crab based on electronic tongue, characterized in that: a discriminant function is constructed according to steps S1 to S5 to identify the origin of Chinese mitten crab:

[0007] Step S1, obtaining a preset number of Chinese mitten crabs from at least two preset production areas, and soaking, washing, and steaming the Chinese mitten crabs from each production area with purified water, and then executing step S2;

[0008] Step S2, based on the preset gram weight, crab meat samples are obtained from the steamed Chinese mitten crabs of each origin, and deionized water is added to each crab meat sample according to a preset ratio to obtain a sample homogenate corresponding to each origin, and impurities and particles in each sample homogenate are removed by a centrifuge and filter paper to obtain a sample solution corresponding to each origin, and then step S3 is performed;

[0009] Step S3, based on the preset capacity, extract part of the sample solution from the sample solution corresponding to each origin as the analysis solution, and put it in a special container for electronic tongue detection, use the electronic tongue to detect the analysis solution one by one, obtain each taste characteristic value corresponding to each analysis solution and the preset taste characteristic, and then execute step S4;

[0010] Step S4, based on the taste characteristic values ​​corresponding to the analyzed solutions, discriminant analysis is performed using SPSS to construct discriminant functions corresponding to the respective origins, and then step S5 is executed;

[0011] Step S5, prepare the Chinese mitten crab to be tested into a sample solution through step S2, obtain the analysis solution and the taste characteristic values ​​corresponding to the preset taste characteristics of the analysis solution through step S3, and respectively bring the taste characteristic values ​​into the discriminant functions constructed in step S4 to determine the origin of the Chinese mitten crab to be tested.

[0012] Furthermore, the Chinese mitten crabs from various origins were soaked in purified water for 1 hour and steamed for 30 minutes.

[0013] Furthermore, in step S2, the crab meat sample obtained is the whole muscle with obvious muscle tissue after removing the gonad part.

[0014] Furthermore, in step S2, deionized water is added to the crab meat sample at a ratio of 1:10.

[0015] Furthermore, in step S3, the test solution is 80 ml of supernatant, the capacity of the electronic tongue detection dedicated container is 100 ml, and each detection time is 100 s and the cleaning time is 30 s.

[0016] Furthermore, when using the electronic tongue for testing, the test temperature should be maintained at 20°C using circulating water.

[0017] Furthermore, in step S5, the function values ​​of the taste characteristic values ​​corresponding to the analysis solution and the preset taste characteristics in each discriminant function are calculated respectively, and the origin corresponding to the discriminant function with the maximum function value is determined as the origin of the Chinese mitten crab to be tested.

[0018] Compared with the prior art, the beneficial effects of the present invention are: based on the electronic tongue, the taste characteristics of the muscle part of the Chinese mitten crab can be objectively and accurately quantified, overcoming the shortcomings of the existing sensory evaluation technology, such as the large subjective influence of sensory evaluators and the high professional requirements for evaluators, and the origin identification of the Chinese mitten crab can be completed based on the accurately measured taste characteristics. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a flow chart of the present invention.

[0020] Figure 2 It is a schematic diagram of the identification result of the embodiment of the present invention. DETAILED DESCRIPTION

[0021] The technical solution of the present invention will be described in detail below with reference to the accompanying drawings.

[0022] refer to Figure 1 , a method for identifying the origin of Chinese mitten crab based on electronic tongue, characterized in that: a discriminant function is constructed according to steps S1 to S5 to identify the origin of Chinese mitten crab:

[0023] Step S1, obtaining 90 Chinese mitten crabs from Yangcheng Lake, Bosten Lake and Zhenlai Lake, and soaking, washing and steaming the Chinese mitten crabs from each origin using purified water, and then executing step S2;

[0024] Step S2, setting the sample weight to 25 g, obtaining all muscles with obvious muscle tissue and gonads removed from steamed Chinese mitten crabs from each origin as crab meat samples, adding deionized water to each crab meat sample at a ratio of 1:10 to obtain sample homogenates corresponding to each origin, and using a centrifuge to centrifuge at 3600 r / min for 15 min and filter paper to remove impurities and particles in each sample homogenate to obtain sample solutions corresponding to each origin, wherein r / min is a unit of rotation speed, which means that the centrifuge rotates 3600 times per minute in this embodiment, and then executing step S3;

[0025] Step S3, extract 80 ml of supernatant from the sample solution corresponding to each origin as the analysis solution, and put it in a 100 ml electronic tongue detection special container, keep the test temperature at 20°C by circulating water, use the electronic tongue to detect the analysis solutions one by one, and each detection time is 100 seconds, and the cleaning time is 30 seconds, and obtain each analysis solution and each taste characteristic value corresponding to the preset taste characteristic. In this embodiment, the taste characteristics are set to 6 basic tastes of fresh, salty, sour, bitter, astringent, and sweet and their aftertaste and richness, and then execute step S4;

[0026] Step S4, based on the taste characteristic values ​​corresponding to the analyzed solutions, discriminant analysis is performed using SPSS to construct discriminant functions corresponding to the respective origins, and then step S5 is executed;

[0027] Specifically, in this embodiment, the discriminant letters of the Yangcheng Lake area, the Bosten Lake area, and the Zhenlai Lake area are as follows:

[0028] Yangcheng Lake: Y YC =59.514X Sourness -499.192X Aftertaste-A +97.399X Umami +14.392X Saltiness -11.008X Sweetness -1065.797

[0029] Bosten Lake: Y BST =65.302X Sourness-545.763X Aftertaste-A +105.706X Umami +16.690X Saltiness -13.393X Sweetness -1254.069

[0030] Zhenlai: Y ZL =99.033X Sourness -772.817X Aftertaste-A +132.889X Umami +16.585X Saltiness -48.858X Sweetness -1788.734

[0031] Among them, Y YC For Yangcheng Lake, Y BST For Bosten Lake, Y ZL For Zhenlai, X Sourness For sour taste, X Aftertaste-A For bitterness, Umami For umami, X Saltiness For salty taste, X Sweetness For sweet taste;

[0032] Step S5, prepare the sample solution of the Chinese mitten crab to be tested by step S2, obtain the analysis solution and the taste characteristic values ​​corresponding to the preset taste characteristics of the analysis solution by step S3, and bring the taste characteristic values ​​into the discriminant functions constructed by step S4, so as to determine the origin of the Chinese mitten crab to be tested, see Figure 2 In this embodiment, the discriminant function constructed is used to identify the origin of the Chinese mitten crab, and the discrimination accuracy is 100%.

[0033] Specifically, in step S5, the function values ​​of the taste characteristic values ​​corresponding to the analysis solution and the preset taste characteristics in each discriminant function are calculated respectively, and the origin corresponding to the discriminant function with the maximum function value is determined as the origin of the Chinese mitten crab to be tested.

[0034] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any way. Any simple modification, change and equivalent structural change made to the above embodiment based on the technical essence of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A method for identifying the origin of Chinese mitten crab based on electronic tongue, characterized in that: According to steps S1 to S5, a discriminant function is constructed to identify the origin of Chinese mitten crab: Step S1, obtaining a preset number of Chinese mitten crabs from at least two preset production areas, and soaking, washing, and steaming the Chinese mitten crabs from each production area with purified water, and then executing step S2; Step S2, based on the preset gram weight, crab meat samples are obtained from the steamed Chinese mitten crabs of each origin, and deionized water is added to each crab meat sample according to a preset ratio to obtain a sample homogenate corresponding to each origin, and impurities and particles in each sample homogenate are removed by a centrifuge and filter paper to obtain a sample solution corresponding to each origin, and then step S3 is performed; Step S3, based on the preset capacity, extract part of the sample solution from the sample solution corresponding to each origin as the analysis solution, and put it in a special container for electronic tongue detection, use the electronic tongue to detect the analysis solution one by one, obtain each taste characteristic value corresponding to each analysis solution and the preset taste characteristic, and then execute step S4; Step S4, based on the taste characteristic values ​​corresponding to the analyzed solutions, discriminant analysis is performed using SPSS to construct discriminant functions corresponding to the respective origins, and then step S5 is executed; Step S5, prepare the Chinese mitten crab to be tested into a sample solution through step S2, obtain the analysis solution and the taste characteristic values ​​corresponding to the preset taste characteristics of the analysis solution through step S3, and respectively bring the taste characteristic values ​​into the discriminant functions constructed in step S4 to determine the origin of the Chinese mitten crab to be tested.

2. The method for identifying the origin of Chinese mitten crab based on electronic tongue according to claim 1, characterized in that: In step S1, the Chinese mitten crabs from various production areas are soaked in purified water for 1 hour and steamed for 30 minutes.

3. The method for identifying the origin of Chinese mitten crab based on electronic tongue according to claim 1, characterized in that: In step S2, the crab meat sample obtained is the whole muscle with obvious muscle tissue after removing the gonad part.

4. The method for identifying the origin of Chinese mitten crab based on electronic tongue according to claim 1, characterized in that: In step S2, deionized water is added to the crab meat sample at a ratio of 1:

10.

5. The method for identifying the origin of Chinese mitten crab based on electronic tongue according to claim 1, characterized in that: In step S3, the test solution is 80 ml of supernatant, the capacity of the electronic tongue detection dedicated container is 100 ml, and each detection time is 100 s and the cleaning time is 30 s.

6. The method for identifying the origin of Chinese mitten crab based on electronic tongue according to claim 1, characterized in that: In step S3, when the electronic tongue is used for detection, the test temperature should be kept at 20° C. using circulating water.

7. The method for identifying the origin of Chinese mitten crab based on electronic tongue according to claim 1, characterized in that: In step S5, the function values ​​of the taste characteristic values ​​corresponding to the analysis solution and the preset taste characteristics in each discriminant function are calculated respectively, and the origin corresponding to the discriminant function with the maximum function value is determined as the origin of the Chinese mitten crab to be tested.

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