Chinese herbal medicine formula and preparation method thereof

Abstract

The invention discloses a Chinese herbal medicine formula and a preparation method thereof, and relates to the technical field of disinfection, the Chinese herbal medicine formula is prepared from the following raw materials by weight: 8-12 parts of rose hydrolat, 1-3 parts of mint hydrolat, 28-42 parts of a Chinese herbal medicine extract, 1-3 parts of chelated platinum ions, 1-3 parts of chelated selenium ions, and 4-10 parts of a composite bacteriostatic agent; the preparation method comprises the following steps: adding the rose hydrolat and the mint hydrolat into the Chinese herbal medicine extract to obtain a stock solution, diluting the stock solution, adding the chelated platinum ions, the chelated selenium ions and the composite bacteriostatic agent, and uniformly stirring to obtain the Chinese herbal medicine formula. The prepared solution can effectively kill bacteria, viruses and fungi, has an excellent killing effect, and does not cause harm to a human body.

Description

A Chinese herbal medicine formula and preparation method thereof Technical Field

[0001] The present invention relates to the technical field of disinfection, and in particular to a Chinese herbal medicine formula and a preparation method thereof. Background Art

[0002] With the development of society and the improvement of people's living standards, people are paying more and more attention to the harm of harmful microorganisms such as bacteria to human health and living environment. Therefore, it is becoming increasingly important to actively explore long-term effective antibacterial materials.

[0003] Chinese herbal disinfectants are less likely to develop drug resistance when used, exhibit antibacterial and anti-inflammatory properties, are low-toxic, and have low or no residual effects. They can also effectively inhibit and kill pathogens. This is because Chinese herbal medicines contain multiple bioactive ingredients that can interfere with multiple aspects of pathogen metabolism, such as energy conversion, DNA, and RNA. Chinese herbal disinfectants are relatively simple to prepare, require simple equipment, are widely available, and are relatively inexpensive, making them easy to use and promote.

[0004] Polymeric antimicrobial agents have a high density of active functional groups, excellent antimicrobial properties, and can kill bacteria through direct contact without releasing active substances. Their antimicrobial properties are long-lasting and stable, with low residual toxicity and a high safety profile. Based on the active functional groups, polymeric antimicrobial agents are primarily classified into quaternary ammonium salts, quaternary phosphonium salts, pyridinium salts, halogenated amines, guanidine salts, and chitosan. Quaternary ammonium salts are a typical representative of polymeric antimicrobial agents, characterized by a positively charged quaternary ammonium nitrogen ion at the center of their structure, surrounded by four substituents and a counteranion. The antimicrobial mechanism of quaternary ammonium salt polymeric antimicrobial agents is that the long carbon chain groups attached to the quaternary ammonium nitrogen ion can penetrate the bacterial lipid cell membrane, achieving a bacterial killing effect.

[0005] Chinese patent document 201810634700.4 discloses a Chinese herbal disinfectant, which is compounded from Chinese herbal medicines such as rhubarb, wormwood leaf, moutan bark, cnidium monnieri, isatis indigotica leaf, sophora flavescens, and gallnut. The disinfectant is prepared by adding water to the uniformly mixed Chinese herbal medicine, boiling it and steaming it with the assistance of ultrasound; then concentrating and precipitating it. The disinfectant has a good inhibitory effect on Escherichia coli, Staphylococcus aureus, and subtilis spores, its stability is less affected by temperature, and it has a significant disinfection effect in clinical field. However, in actual application, it was found that the antibacterial effect of the disinfectant was not ideal and its scope of application was not wide. Summary of the Invention

[0006] In order to address the deficiencies in the prior art, the present invention aims to provide a Chinese herbal medicine formula and a preparation method thereof, which has good product quality stability, readily available materials, low cost, excellent bactericidal effect, and does not cause harm to the human body.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions:

[0008] A Chinese herbal medicine formula, comprising the following raw materials, by weight: 8-12 parts of rose hydrosol, 1-3 parts of mint hydrosol, 28-42 parts of Chinese herbal medicine extract, 1-3 parts of chelated platinum ions, 1-3 parts of chelated selenium ions, and 4-10 parts of a composite antibacterial agent;

[0009] The preparation method of the composite antibacterial agent comprises the following steps:

[0010] (1) calcining bentonite and then cooling it to room temperature, crushing it and dispersing it in an ethanol aqueous solution, adjusting the pH with hydrochloric acid, then adding 3-mercaptopropyltriethoxysilane, stirring the reaction, centrifuging the product, washing it, and drying it to obtain mercapto-bentonite;

[0011] (2) Dodecafluoroheptyl methacrylate and methacryloyloxyethyl trimethylammonium chloride are added to N,N-dimethylformamide and stirred to obtain a mixed solution. Then, thiol-bentonite is dispersed in the mixed solution, ultrasonically dispersed, a photoinitiator is added, and the mixture is reacted under ultraviolet light. The product is centrifuged, washed with isopropyl alcohol, and dried to obtain a composite antibacterial agent.

[0012] Preferably, in step (1), the concentration of the ethanol aqueous solution is 50-75 wt %, and the pH is adjusted to 3-5.

[0013] Preferably, in step (1), the weight ratio of bentonite, ethanol aqueous solution, and 3-mercaptopropyltriethoxysilane is 10:30-50:2-5.

[0014] Preferably, in step (1), the calcination condition is 150-300° C. for 1-2 h; and the stirring reaction condition is 30-45° C. in a N 2 atmosphere for 5-8 h.

[0015] Preferably, in step (2), the weight ratio of dodecafluoroheptyl methacrylate, methacryloyloxyethyltrimethylammonium chloride, N,N-dimethylformamide, and mercapto-bentonite is 4-7:1-3:30-50:10.

[0016] Preferably, in step (2), the photoinitiator is one or more of benzoin dimethyl ether, benzoin ethyl ether, and benzoin butyl ether.

[0017] Preferably, in step (2), the ultrasonic dispersion time is 15-30 min, the main ultraviolet wavelength is 360-365 nm, and the ultraviolet light intensity is 45-75 mW / cm 2 , irradiation time is 60~90min, and reaction temperature is 35~45℃.

[0018] Preferably, the preparation method of the Chinese herbal medicine extract comprises the following steps: weighing 1-2 parts of safflower, 8-12 parts of peach kernel, 8-12 parts of forsythia, 8-13 parts of chrysanthemum, 7-13 parts of perilla, 18-25 parts of platycodon, 8-11 parts of star anise, 9-14 parts of costus root, 7-12 parts of podocarp, 10-12 parts of atractylodes, 10-12 parts of white atractylodes, 9-13 parts of green peel, 10-12 parts of cloves, 4-6 parts of zanthoxylum bungeanum, and 13-17 parts of tea leaves, adding the mixture to a reaction vessel, then adding 500 parts of deionized water, and distilling and extracting at 94-97°C for 1.5-3h to obtain the Chinese herbal medicine extract.

[0019] The present invention also claims protection for a preparation method of the Chinese herbal formula, comprising the following steps: adding rose hydrosol and mint hydrosol to a Chinese herbal extract to obtain a stock solution, diluting the stock solution, adding chelated platinum ions, chelated selenium ions and a composite antibacterial agent, and stirring evenly to obtain the Chinese herbal formula.

[0020] Preferably, the stock solution is diluted 10 to 20 times.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] Safflower: It promotes blood circulation and menstruation, dissipates blood stasis and relieves pain. It helps to treat amenorrhea, dysmenorrhea, lochia retention, chest pain, heart pain due to blood stasis, chest and flank pain, traumatic injuries, and sores and swelling.

[0023] Peach kernel: It has the effects of promoting blood circulation and removing blood stasis, moistening the intestines and promoting bowel movements, and relieving cough and asthma. It is used to treat amenorrhea, dysmenorrhea, abdominal masses, lung and intestinal abscesses, traumatic injuries, dry intestines and constipation, and cough and asthma.

[0024] Forsythia: Clears away heat, detoxifies, disperses stagnation, and reduces swelling. Treats febrile diseases, erysipelas, rashes, carbuncles, ulcers, scrofula, and stranguria.

[0025] Chrysanthemum: dispels wind, clears heat, improves eyesight, and detoxifies. Treats headaches, dizziness, red eyes, chest heat, furuncles, and swelling.

[0026] Perilla: The leaves are diaphoretic, antitussive, aromatic stomachic and diuretic, with analgesic, sedative and detoxifying effects, and can treat colds; the stems have the function of calming qi and stabilizing pregnancy; the seeds can relieve cough, eliminate phlegm, relieve asthma, and disperse mental dullness.

[0027] Platycodon grandiflorum: clears the lungs, relieves sore throat, eliminates phlegm, and discharges pus. It is used for cough with excessive phlegm, chest tightness, sore throat, hoarseness, lung abscess with vomiting pus, and sores with pus that do not break.

[0028] Star anise: It is warm in nature and pungent in flavor. It has the effects of warming yang, dispelling cold, regulating qi and relieving pain. It is used to treat cold vomiting, abdominal pain caused by cold hernia, low back pain caused by kidney deficiency, and dry and wet athlete's foot.

[0029] Costus root: Promotes qi circulation and relieves pain, strengthens the spleen and aids digestion. Used for chest and abdominal distension and pain, heaviness after diarrhea, indigestion, and loss of appetite.

[0030] Patchouli: Invigorates Qi, harmonizes the stomach, dispels filth and dampness. Treats colds, summer heat, fever, headaches, chest and abdominal distension, vomiting and diarrhea, malaria, dysentery, and bad breath.

[0031] Cangshu: Strengthens the spleen, dries dampness, relieves depression, and dispels filth. Treats dampness that blocks the spleen, fatigue and sleepiness, abdominal distension, loss of appetite, vomiting, diarrhea, dysentery, malaria, phlegm and fluid retention, edema, seasonal colds, wind-cold-dampness arthritis, foot paralysis, and night blindness.

[0032] Atractylodes macrocephala: tonifies the spleen, benefits the stomach, eliminates dampness, harmonizes the middle, and stabilizes the fetus. Treats spleen and stomach qi deficiency, loss of appetite, fatigue and shortness of breath, bloating, diarrhea, phlegm and fluid retention, edema, jaundice, dampness and arthritis, dysuria, dizziness, spontaneous sweating, and fetal restlessness.

[0033] Qingpi: Soothes the liver and dispels qi, eliminates stagnation and resolves stagnation. Used for chest and flank pain, hernia, breast nodules, mastitis, and abdominal pain caused by food stagnation.

[0034] Clove: warms the middle, warms the kidneys, and reduces adverse reactions. It is used to treat hiccups, vomiting, nausea, diarrhea, cold pain in the heart and abdomen, abdominal distension, hernia, and ringworm.

[0035] Zanthoxylum bungeanum: warms the middle and dispels cold, removes dampness, relieves pain, kills insects, and detoxifies fishy smell. It is used to treat indigestion, cold pain in the heart and abdomen, vomiting, belching, cough and shortness of breath, wind-cold-dampness arthritis, diarrhea, dysentery, hernia pain, toothache, ascariasis, pinworm disease, vaginal itching, and scabies.

[0036] Tea: Clears the head and eyes, relieves thirst, resolves phlegm, helps digestion, promotes urination, and detoxifies. Treats headaches, blurred vision, excessive sleep, restlessness and thirst, food stagnation and phlegm, malaria, and dysentery.

[0037] 1) The present invention discloses a Chinese herbal medicine formula, which uses plant components with anti-inflammatory, antioxidant, anti-allergic, infection-reducing, and immune-enhancing effects, composite antibacterial components, and chelated platinum ions and chelated selenium ions that catalyze and enhance the performance of the herbal medicine as raw materials. The synthesized care solution has excellent bactericidal and antibacterial effects and can effectively moisturize and maintain human skin. It can be sprayed in a convenient and quick manner.

[0038] 2) The present invention provides a composite antibacterial agent. First, bentonite is organically modified with 3-mercaptopropyltriethoxysilane to obtain mercapto-bentonite, which can effectively improve the dispersibility of bentonite in Chinese herbal medicine extracts and prevent bentonite from aggregating and settling in the medicine. Then, dodecafluoroheptyl methacrylate and methacryloyloxyethyltrimethylammonium chloride are grafted onto the mercapto-bentonite through a mercapto-ene reaction under ultraviolet light to obtain a composite antibacterial agent. Methacryloyloxyethyltrimethylammonium chloride can change the permeability of bacterial membranes. It kills bacteria and has good surface activity, gathering on the surface of bacteria to affect bacterial metabolism. In addition, it can denature proteins and inactivate bacterial enzyme systems. It is safe and non-toxic to the human body, non-irritating to the skin, and will not cause bacteria to develop drug resistance, so it can bring excellent antibacterial activity; dodecafluoroheptyl methacrylate has excellent hydrophobic properties, which can prevent the initial adhesion of bacteria and proteins and inhibit the formation of biofilms, thereby synergistically inhibiting and killing bacteria with methacryloyloxyethyltrimethylammonium chloride. Implementation Method

[0039] In order to make the purpose, technical solutions and advantages of the present invention clearer, the present invention is further described in detail below in conjunction with the embodiments. Of course, the specific embodiments described here are only used to explain the present invention and are not used to limit the present invention.

[0040] Unless otherwise specified, the chemical reagents and materials in the present invention are purchased from commercial sources or synthesized from commercially purchased raw materials.

[0041] Bentonite was purchased from Haoyuan Bentonite Factory in Fangzi District, Weifang City, with a particle size of 4000-5000 mesh;

[0042] Rose hydrosol and mint hydrosol were purchased from Guangzhou Zhonghe International Biotechnology Co., Ltd.

[0043] Chelated platinum ions and chelated selenium ions were purchased from Asia-Pacific Technology Group.

[0044] The present invention will be further described below with reference to specific examples. Example

[0045] A method for preparing a Chinese herbal medicine formula comprises the following steps:

[0046] (1) 10 g of bentonite was calcined at 300 °C for 2 h and then cooled to room temperature. The mixture was crushed and dispersed into 50 g of a 70 wt% ethanol aqueous solution. The pH was adjusted to 5 with hydrochloric acid. Then, 5 g of 3-mercaptopropyltriethoxysilane was added. The mixture was stirred at 45 °C in a nitrogen atmosphere for 8 h. The product was centrifuged, washed, and dried to obtain mercapto-bentonite.

[0047] (2) Add 7g dodecafluoroheptyl methacrylate and 3g methacryloyloxyethyl trimethylammonium chloride to 50g N,N-dimethylformamide and stir to obtain a mixed solution. Then disperse 10g of thiolated bentonite into the mixed solution and ultrasonically disperse for 30min. Add benzoin butyl ether and conduct ultrasonication at a main wavelength of 365nm and an intensity of 60mW / cm 2 The reaction was carried out under ultraviolet light at 45°C for 90 minutes, and the product was centrifuged, washed with isopropanol, and dried to obtain a composite antibacterial agent;

[0048] (3) Weigh 2 g of safflower, 10 g of peach kernel, 10 g of forsythia, 11 g of chrysanthemum, 10 g of perilla, 22 g of platycodon, 9 g of star anise, 12 g of costus root, 8 g of agave root, 11 g of atractylodes, 11 g of white atractylodes, 11 g of green peel, 11 g of clove, 5 g of Zanthoxylum bungeanum, and 16 g of tea leaves, add them to a reaction vessel, then add 500 g of deionized water, and distill and extract at 96 ° C for 2.5 h to obtain the Chinese herbal medicine extract;

[0049] (4) Add 12g of rose hydrosol and 3g of mint hydrosol to 42g of Chinese herbal medicine extract to obtain a stock solution. Dilute the stock solution 20 times and then add 3g of chelated platinum ions, 3g of chelated selenium ions and 10g of compound antibacterial agent. Stir well to obtain the Chinese herbal medicine formula. Example

[0050] A method for preparing a Chinese herbal medicine formula comprises the following steps:

[0051] (1) 10 g of bentonite was calcined at 200 °C for 1.5 h and then cooled to room temperature. The mixture was crushed and dispersed in 40 g of a 70 wt% ethanol aqueous solution. The pH was adjusted to 4 with hydrochloric acid. Then, 4 g of 3-mercaptopropyltriethoxysilane was added. The mixture was stirred at 35 °C in a nitrogen atmosphere for 7 h. The product was centrifuged, washed, and dried to obtain mercapto-bentonite.

[0052] (2) Add 5 g of dodecafluoroheptyl methacrylate and 2 g of methacryloyloxyethyl trimethylammonium chloride to 40 g of N,N-dimethylformamide and stir to obtain a mixed solution. Then disperse 10 g of thiolated bentonite into the mixed solution and ultrasonically disperse for 20 min. Add benzoin butyl ether and conduct ultrasonication at a main wavelength of 365 nm and an intensity of 60 mW / cm 2 The reaction was carried out under ultraviolet light at 40°C for 75 minutes, and the product was centrifuged, washed with isopropanol, and dried to obtain a composite antibacterial agent;

[0053] (3) Weigh 2 g of safflower, 10 g of peach kernel, 10 g of forsythia, 11 g of chrysanthemum, 10 g of perilla, 22 g of platycodon, 9 g of star anise, 12 g of costus root, 8 g of agave root, 11 g of atractylodes, 11 g of white atractylodes, 11 g of green peel, 11 g of clove, 5 g of Zanthoxylum bungeanum, and 16 g of tea leaves, add them to a reaction vessel, then add 500 g of deionized water, and distill and extract at 96 ° C for 2.5 h to obtain the Chinese herbal medicine extract;

[0054] (4) Add 10g of rose hydrosol and 2g of mint hydrosol to 35g of Chinese herbal medicine extract to obtain the stock solution. Dilute the stock solution 20 times and add 2g of chelated platinum ions, 2g of chelated selenium ions and 7g of compound antibacterial agent. Stir well to obtain the Chinese herbal medicine formula. Example

[0055] A method for preparing a Chinese herbal medicine formula comprises the following steps:

[0056] (1) 10 g of bentonite was calcined at 150 °C for 1 h and then cooled to room temperature. The mixture was crushed and dispersed into 30 g of a 70 wt% ethanol aqueous solution. The pH was adjusted to 3 with hydrochloric acid. Then, 2 g of 3-mercaptopropyltriethoxysilane was added. The mixture was stirred at 30 °C in a nitrogen atmosphere for 5 h. The product was centrifuged, washed, and dried to obtain mercapto-bentonite.

[0057] (2) Add 4 g of dodecafluoroheptyl methacrylate and 1 g of methacryloyloxyethyl trimethylammonium chloride to 30 g of N,N-dimethylformamide and stir to obtain a mixed solution. Then disperse 10 g of thiolated bentonite into the mixed solution and ultrasonically disperse for 15 min. Add benzoin butyl ether and conduct ultrasonic dispersion at a main wavelength of 365 nm and an intensity of 60 mW / cm 2 The reaction was carried out under ultraviolet light at 35°C for 60 minutes, and the product was centrifuged, washed with isopropanol, and dried to obtain a composite antibacterial agent;

[0058] (3) Weigh 2 g of safflower, 10 g of peach kernel, 10 g of forsythia, 11 g of chrysanthemum, 10 g of perilla, 22 g of platycodon, 9 g of star anise, 12 g of costus root, 8 g of agave root, 11 g of atractylodes, 11 g of white atractylodes, 11 g of green peel, 11 g of clove, 5 g of Zanthoxylum bungeanum, and 16 g of tea leaves, add them to a reaction vessel, then add 500 g of deionized water, and distill and extract at 96 ° C for 2.5 h to obtain the Chinese herbal medicine extract;

[0059] (4) Add 8g of rose hydrosol and 1g of mint hydrosol to 28 Chinese herbal medicine extracts to obtain a stock solution. Dilute the stock solution 20 times and then add 1g of chelated platinum ions, 1g of chelated selenium ions and 4g of compound antibacterial agent. Stir well to obtain the Chinese herbal medicine formula.

[0060] A method for preparing a Chinese herbal medicine formula comprises the following steps:

[0061] (1) 10 g of bentonite was calcined at 300 °C for 2 h and then cooled to room temperature. The mixture was crushed and dispersed into 50 g of a 70 wt% ethanol aqueous solution. The pH was adjusted to 5 with hydrochloric acid. Then, 5 g of 3-mercaptopropyltriethoxysilane was added. The mixture was stirred at 45 °C in a nitrogen atmosphere for 8 h. The product was centrifuged, washed, and dried to obtain mercapto-bentonite.

[0062] (2) Add 7g dodecafluoroheptyl methacrylate to 50g N,N-dimethylformamide and stir to obtain a mixed solution. Then disperse 10g of thiolated bentonite into the mixed solution and ultrasonically disperse for 30min. Add benzoin butyl ether and conduct ultrasonication at a main wavelength of 365nm and an intensity of 60mW / cm 2 The reaction was carried out under ultraviolet light at 45°C for 90 minutes, and the product was centrifuged, washed with isopropanol, and dried to obtain an antibacterial agent;

[0063] (3) Weigh 2 g of safflower, 10 g of peach kernel, 10 g of forsythia, 11 g of chrysanthemum, 10 g of perilla, 22 g of platycodon, 9 g of star anise, 12 g of costus root, 8 g of agave root, 11 g of atractylodes, 11 g of white atractylodes, 11 g of green peel, 11 g of clove, 5 g of Zanthoxylum bungeanum, and 16 g of tea leaves, add them to a reaction vessel, then add 500 g of deionized water, and distill and extract at 96 ° C for 2.5 h to obtain the Chinese herbal medicine extract;

[0064] (4) Add 12g of rose hydrosol and 3g of mint hydrosol to 42g of Chinese herbal medicine extract to obtain a stock solution. Dilute the stock solution 20 times, add 3g of chelated platinum ions, 3g of chelated selenium ions and 10g of antibacterial agent, and stir evenly to obtain the Chinese herbal medicine formula.

[0065] A method for preparing a Chinese herbal medicine formula comprises the following steps:

[0066] (1) 10 g of bentonite was calcined at 300 °C for 2 h and then cooled to room temperature. The mixture was crushed and dispersed into 50 g of a 70 wt% ethanol aqueous solution. The pH was adjusted to 5 with hydrochloric acid. Then, 5 g of 3-mercaptopropyltriethoxysilane was added. The mixture was stirred at 45 °C in a nitrogen atmosphere for 8 h. The product was centrifuged, washed, and dried to obtain mercapto-bentonite.

[0067] (2) Add 3 g of methacryloyloxyethyl trimethylammonium chloride to 50 g of N,N-dimethylformamide and stir to obtain a mixed solution. Then disperse 10 g of thiolated bentonite into the mixed solution and ultrasonically disperse for 30 min. Add benzoin butyl ether and conduct ultrasonication at a main wavelength of 365 nm and an intensity of 60 mW / cm 2 The reaction was carried out under ultraviolet light at 45°C for 90 minutes, and the product was centrifuged, washed with isopropanol, and dried to obtain an antibacterial agent;

[0068] (3) Weigh 2 g of safflower, 10 g of peach kernel, 10 g of forsythia, 11 g of chrysanthemum, 10 g of perilla, 22 g of platycodon, 9 g of star anise, 12 g of costus root, 8 g of agave root, 11 g of atractylodes, 11 g of white atractylodes, 11 g of green peel, 11 g of clove, 5 g of Zanthoxylum bungeanum, and 16 g of tea leaves, add them to a reaction vessel, then add 500 g of deionized water, and distill and extract at 96 ° C for 2.5 h to obtain the Chinese herbal medicine extract;

[0069] (4) Add 12g of rose hydrosol and 3g of mint hydrosol to 42g of Chinese herbal medicine extract to obtain a stock solution. Dilute the stock solution 20 times, add 3g of chelated platinum ions, 3g of chelated selenium ions and 10g of antibacterial agent, and stir evenly to obtain the Chinese herbal medicine formula.

[0070] A method for preparing a Chinese herbal medicine formula comprises the following steps:

[0071] (1) 10 g of bentonite was calcined at 300 °C for 2 h and then cooled to room temperature. The mixture was crushed and dispersed into 50 g of a 70 wt% ethanol aqueous solution. The pH was adjusted to 5 with hydrochloric acid. Then, 5 g of 3-mercaptopropyltriethoxysilane was added. The mixture was stirred at 45 °C in a nitrogen atmosphere for 8 h. The product was centrifuged, washed, and dried to obtain mercapto-bentonite.

[0072] (2) Weigh 2 g of safflower, 10 g of peach kernel, 10 g of forsythia, 11 g of chrysanthemum, 10 g of perilla, 22 g of platycodon, 9 g of star anise, 12 g of costus root, 8 g of agave root, 11 g of atractylodes, 11 g of white atractylodes, 11 g of green peel, 11 g of clove, 5 g of Zanthoxylum bungeanum, and 16 g of tea leaves, add them to a reaction vessel, then add 500 g of deionized water, and distill and extract at 96 ° C for 2.5 h to obtain the Chinese herbal medicine extract;

[0073] (3) Add 12g of rose hydrosol and 3g of mint hydrosol to 42g of Chinese herbal medicine extract to obtain a stock solution. Dilute the stock solution 20 times and then add 3g of chelated platinum ions, 3g of chelated selenium ions and 10g of thiolated bentonite. Stir well to obtain the Chinese herbal medicine formula.

[0074] Escherichia coli and Staphylococcus aureus were selected as experimental strains and activated in broth culture at 37°C on a shaker at 150 rpm for 8 hours before use. The herbal products prepared in Examples 1-3 and Comparative Examples 1-3, sterilized at 121°C for 20 minutes, were added to sterile broth culture at a concentration of 0.05% (w / v). Under aseptic conditions, 1 mL of the activated bacterial solution (bacterial concentration of 109 cfu / mL) was added to 49 mL of the above-prepared culture medium and incubated at 37°C on a shaker at 150 rpm for 6 hours. The solution was then diluted and characterized by plate count according to GB 4789.2-2022, "National Food Safety Standard for Food Microbiological Examination - Determination of Total Colony Counts." The sterilization rate was calculated according to the following formula: sterilization rate = (bacterial count in blank group - bacterial count in experimental group) / bacterial count in blank group, where bacterial count is expressed in cfu / mL. The test results are shown in Table 1.

[0075] Table 1 Bactericidal rate of Chinese herbal medicine formula (%)

[0076] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.

Claims

1. A Chinese herbal medicine formula, characterized in that: The preparation is made from the following raw materials, in parts by weight: 8-12 parts of rose hydrosol, 1-3 parts of mint hydrosol, 28-42 parts of Chinese herbal medicine extract, 1-3 parts of chelated platinum ions, 1-3 parts of chelated selenium ions, and 4-10 parts of a composite antibacterial agent. The preparation method of the composite antibacterial agent comprises the following steps: (1) calcining bentonite and then cooling it to room temperature, crushing it and dispersing it in an ethanol aqueous solution, adjusting the pH with hydrochloric acid, then adding 3-mercaptopropyltriethoxysilane, stirring the reaction, centrifuging the product, washing it, and drying it to obtain mercapto-bentonite; (2) Dodecafluoroheptyl methacrylate and methacryloyloxyethyl trimethylammonium chloride are added to N,N-dimethylformamide and stirred to obtain a mixed solution. Then, thiol-bentonite is dispersed in the mixed solution, ultrasonically dispersed, a photoinitiator is added, and the mixture is reacted under ultraviolet light. The product is centrifuged, washed with isopropyl alcohol, and dried to obtain a composite antibacterial agent.

2. The Chinese herbal medicine formula according to claim 1, characterized in that: In step (1), the concentration of the ethanol aqueous solution is 50-75 wt %, and the pH is adjusted to 3-5.

3. The Chinese herbal medicine formula according to claim 1, characterized in that In step (1), the weight ratio of bentonite, ethanol aqueous solution and 3-mercaptopropyltriethoxysilane is 10:30-50:2-5.

4. The Chinese herbal medicine formula according to claim 1, characterized in that: In step (1), the calcination condition is 150-300° C. for 1-2 h; and the stirring reaction condition is 30-45° C. in a N 2 atmosphere for 5-8 h.

5. The Chinese herbal medicine formula according to claim 1, characterized in that: In step (2), the weight ratio of dodecafluoroheptyl methacrylate, methacryloyloxyethyl trimethylammonium chloride, N,N-dimethylformamide, and mercapto-bentonite is 4-7:1-3:30-50:

10.

6. The Chinese herbal medicine formula according to claim 1, characterized in that: In step (2), the photoinitiator is one or more of benzoin dimethyl ether, benzoin ethyl ether, and benzoin butyl ether.

7. The Chinese herbal medicine formula according to claim 1, characterized in that: In step (2), the ultrasonic dispersion time is 15-30 min, the main UV wavelength is 360-365 nm, and the UV intensity is 45-75 mW / cm 2 , irradiation time is 60~90min, and reaction temperature is 35~45℃.

8. The Chinese herbal medicine formula according to claim 1, characterized in that: The preparation method of the Chinese herbal medicine extract comprises the following steps: weighing 1-2 parts of safflower, 8-12 parts of peach kernel, 8-12 parts of forsythia, 8-13 parts of chrysanthemum, 7-13 parts of perilla, 18-25 parts of platycodon, 8-11 parts of star anise, 9-14 parts of costus root, 7-12 parts of agave root, 10-12 parts of atractylodes, 10-12 parts of white atractylodes, 9-13 parts of green peel, 10-12 parts of clove, 4-6 parts of zanthoxylum bungeanum, and 13-17 parts of tea leaves, adding the mixture into a reaction container, then adding 500 parts of deionized water, and performing distillation extraction at 94-97°C for 1.5-3 hours to obtain the Chinese herbal medicine extract.

9. A method for preparing the Chinese herbal medicine formula according to any one of claims 1 to 8, characterized in that: The method comprises the following steps: adding rose hydrosol and mint hydrosol into Chinese herbal medicine extract to obtain stock solution, diluting the stock solution, adding chelated platinum ions, chelated selenium ions and a composite antibacterial agent, and stirring evenly to obtain a Chinese herbal medicine formula.

10. The preparation method according to claim 9, characterized in that The dilution ratio of the stock solution is 10~20 times.