Cuttage method of cyclobalanopsis glauca

Abstract

The invention discloses a cuttage method of cyclobalanopsis glauca, and belongs to the technical field of cuttage, the method comprises the following steps: (1) treatment of cutting slips: smearing a treating agent on cutting slips cuts, carrying out standing treatment for 30-40 min, and removing the treating agent on the cutting slips cuts after the treatment is completed to obtain treated cutting slips; (2) cutting: filling a hole tray with a matrix, disinfecting the hole tray with a 1% potassium permanganate solution, and then cutting the treated cutting slips into the hole tray; and (3) cuttage management: after cuttage is finished, thoroughly watering, spraying 1000-fold potassium fulvate once, then building a sunshade net above a hole tray, keeping the temperature and the humidity, and spraying 0.1% carbendazim solution once every week, so that the problem of difficult rooting of the cuttings caused by excessive tannin in the cuttings is solved.

Description

Technical Field

[0001] The invention relates to the technical field of Cyclobalanopsis xichouensis cuttings, in particular to a Cyclobalanopsis xichouensis cutting method. Background Art

[0002] Quercus sichourensis (Hu) CC Huang & Y.T. Chang is a species of the Fagaceae family and the genus Quercus. Quercus sichourensis is an evergreen tree with a beautiful tree shape, bright red young leaves, and large and beautiful fruits. It is an excellent ornamental tree. It is mainly distributed in Xichou County and Funing County in the southeast of Yunnan Province, and Ceheng County in Guizhou Province. There are only dozens of wild populations. The propagation of Quercus sichourensis by cuttings can not only maintain genetic traits, but also achieve rapid seedling cultivation. However, Quercus sichourensis contains a lot of tannins, which have a certain inhibitory effect on the rooting of cuttings of Quercus sichourensis. During the cutting process, it will be retained on the surface of the cutting cut, affecting the water absorption of the cutting, thereby reducing the rooting ability and affecting the survival rate of the cutting. At present, most of the cuttings are soaked in polyvinyl pyrrolidone solution and acetone solution to remove the tannins in the cuttings and promote the rooting of the cuttings. However, the tannin removal efficiency is low, the rooting rate of cuttings is still low, and improper handling may cause damage to the cuttings, further reducing their rooting ability.

[0003] Therefore, the present invention provides a cutting method for Cyclobalanopsis xichouensis, which reduces the content of tannin in the cuttings and promotes the rooting of the cuttings. Summary of the invention

[0004] In view of this, the object of the present invention is to provide a cutting method for Cyclobalanopsis xichouensis, so as to solve the problem that too much tannin in the cuttings causes difficulty in rooting the cuttings.

[0005] The present invention solves the above technical problems by the following technical means:

[0006] A cutting method for Cyclobalanopsis xichouensis, the method comprising the following steps:

[0007] (1) Treatment of cuttings: Select semi-lignified Cyclobalanopsis glauca branches with full leaf buds as cuttings, apply the treatment agent to the cutting cuts, and let them stand for 30-40 minutes. After the treatment is completed, remove the treatment agent from the cutting cuts to obtain treated cuttings;

[0008] (2) Cutting: Mix perlite and peat soil in a volume ratio of 1:2 to obtain a cutting medium, then fill the plug tray with the cutting medium and compact it slightly, and disinfect the plug tray with 1% potassium permanganate solution, then insert the treated cuttings into the plug tray, insert one cutting into each hole, and the cutting depth is 3-4 cm;

[0009] (3) Cutting management: After the cutting is completed, water thoroughly and spray 1000 times potassium humate once, then cover the hole tray with a shade net to maintain the temperature and humidity, spray 0.1% carbendazim solution once a week, and transplant after 70-80 days of cultivation.

[0010] Furthermore, the method for processing the cuttings in step (1) is as follows: the branches of Cyclobalanopsis glauca are cut to a length of 8-10 cm, 2-3 leaves at the top are retained, and 50-60% of the area of ​​each leaf is retained; the upper incision of the cuttings is flat, and the lower end is beveled.

[0011] Keeping less leaf area on the cuttings can effectively reduce the transpiration of the cuttings, thereby reducing water loss and avoiding excessive water loss in the cuttings, which leads to a reduction in rooting ability. The cuttings have a flat cut with a small surface area, which can further reduce water evaporation to keep the cuttings moist. The lower end bevel can increase the contact area between the cuttings and the treatment agent, thereby improving the treatment effect.

[0012] Furthermore, in step (3), the temperature is maintained at 20-25° C. and the humidity is maintained at 80-85%.

[0013] Further, the preparation method of the treatment agent is as follows:

[0014] S1: adding benzoic acid to water, heating and stirring until completely dissolved to obtain a 5wt% benzoic acid solution; mixing the benzoic acid solution, palm oil, rosin glycerol ester and volcanic mud evenly, stirring at 50-80°C for 40-50min, and cooling to obtain modified volcanic mud;

[0015] S2: adding gliadin to a 70 wt % ethanol solution and stirring until completely dissolved, then adding water to prepare a 2 wt % gliadin solution, then adding xylan and sodium laurate to the gliadin solution, and mixing well to obtain solution A;

[0016] S3: The modified volcanic mud and solution A are mixed and stirred evenly, and the water content is adjusted to 40-45% to obtain a treatment agent.

[0017] Furthermore, in step S1 of the method for preparing the treating agent, the mass ratio of benzoic acid solution, palm oil, rosin glycerol ester, and volcanic mud is (20-25): (3-4): (2-2.5): (20-25).

[0018] Furthermore, in step S2 of the method for preparing the treating agent, the mass ratio of gliadin, xylan and sodium laurate is (1-1.5): (1-1.2): (1.2-1.5).

[0019] Furthermore, in step S3 of the method for preparing the treatment agent, the mass ratio of the modified volcanic mud to the solution A is (20-25): (6-8).

[0020] Furthermore, the amount of the treatment agent used is 10-15 g / plant.

[0021] The treatment agent is applied to the cutting cut, the treatment agent is completely wrapped around the cutting cut, and then taken out for static treatment. During the static treatment process, the gliadin in the treatment agent can combine with the tannins retained on the cutting interface surface to form a tannin-protein complex precipitation, reducing the tannin content at the cutting cut; a certain amount of xylan is also added to the treatment agent, which can combine with the tannins on the cutting cut surface through hydrogen bonding to form a precipitation complex, thereby further reducing the tannin content at the cutting cut, reducing the inhibitory effect of tannins on the rooting of the cuttings, and improving the rooting rate of the cuttings.

[0022] However, the tannin at the cutting cut generates precipitation under the action of the treatment agent, which will cover the surface of the cutting cut, reduce the water absorption capacity of the cutting, thereby affecting the rooting ability and causing difficulty in rooting. Therefore, volcanic mud is added to the treatment agent. The volcanic mud has a strong adsorption capacity and can adsorb the tannin composite precipitate blocked on the surface of the cutting into the treatment agent. However, during the treatment process, the volcanic mud has a strong adsorption capacity and will absorb the moisture of the cutting, causing the cutting to lose water and reduce the rooting rate. In addition, since the volcanic mud easily loses its original viscosity during the process of water loss and solidification and falls off from the cutting, the treatment agent acts on the cutting for a short time. Therefore, it is necessary to treat the volcanic mud in the treatment agent to increase the hydrophobicity of the volcanic mud, thereby reducing the absorption of water in the cutting by the volcanic mud; at the same time, the adhesion of the treatment agent should be increased to prevent the treatment agent from falling off too quickly. Palm oil can combine with the hydroxyl groups on the surface of volcanic mud to reduce the hydrophilicity of volcanic mud, thereby reducing the absorption of water by volcanic mud to the cuttings; benzoic acid can also reduce the water absorption of volcanic mud, and benzoic acid can also promote the rooting of cuttings and improve the rooting rate of cuttings. In addition, using rosin glycerol ester to treat volcanic mud can increase the surface tension of volcanic mud, further increase the hydrophobicity of volcanic mud, and prevent volcanic mud from absorbing water from cuttings; at the same time, rosin glycerol ester can also increase the adhesion of the adsorbent to prevent the treatment agent from falling off too quickly after drying and reducing the treatment effect. In addition, sodium laurate is also added to the treatment agent, which adheres to the surface of the cuttings through hydrophobic action, and forms a sticky film on the surface of the cuttings during the gradual drying of the treatment agent, so that the treatment agent adheres to the cuttings to prevent the treatment agent from falling off during the treatment period. After the treatment is completed, the treatment agent at the cutting incision is removed, and the tannin complex is removed together with the treatment agent, thereby reducing the coverage and retention of the tannin complex at the cutting incision and promoting the rooting of the cuttings.

[0023] Beneficial effects:

[0024] The present invention provides a cutting method of Cyclobalanopsis xichouensis, which reduces the tannin retained on the cutting surface of the cutting by applying a treatment agent to the cutting incision, thereby promoting the rooting of the cutting and further improving the cutting survival rate of Cyclobalanopsis xichouensis. At the same time, the present invention is simple to operate and does not cause damage to the cutting. At the same time, the time from cutting of Cyclobalanopsis xichouensis to plant maturity is shortened, the breeding cycle is accelerated, and the development of the Cyclobalanopsis xichouensis breeding industry is promoted. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 :Xichou Cyclobalanopsis illustration DETAILED DESCRIPTION

[0026] The present invention will be described in detail below with reference to specific embodiments:

[0027] Embodiment 1:

[0028] Preparation of treatment agent:

[0029] S1: Add benzoic acid to water, heat and stir until completely dissolved to obtain a 5wt% benzoic acid solution; mix 20g of benzoic acid solution, 3g of palm oil, 2g of rosin glycerol ester and 20g of volcanic mud, stir at 50°C for 40min, and cool to obtain modified volcanic mud;

[0030] S2: 1.2 g of gliadin was added to a 70 wt % ethanol solution and stirred until completely dissolved, and then water was added to obtain a 2 wt % gliadin solution, and then 1 g of xylan and 1.2 g of sodium laurate were added to the gliadin solution and mixed to obtain a solution A;

[0031] S3: The modified volcanic mud and solution A are mixed evenly in a mass ratio of 20:6, and the water content is adjusted to 40% to obtain a treatment agent.

[0032] Cutting method of Cyclobalanopsis xichouensis:

[0033] (1) Treatment of cuttings: Select semi-lignified Cyclobalanopsis glauca branches with full leaf buds, cut the Cyclobalanopsis glauca branches to a length of 8 cm, retain the top two leaves, and retain 50% of the area of ​​each leaf. The cuttings are cut flat and the lower end is cut obliquely to obtain cuttings. Then, the treatment agent is applied to the cutting of the cuttings. The amount used is 10 g / plant, and then the cuttings are left to stand for 30 minutes. After the treatment is completed, the treatment agent at the cutting of the cuttings is gently tapped to remove the treatment agent, thereby obtaining treated cuttings;

[0034] (2) Cutting: Mix perlite and peat soil in a volume ratio of 1:2 to obtain a cutting medium, then fill the plug tray with the cutting medium and compact it slightly, and disinfect the plug tray with 1% potassium permanganate solution, then insert the treated cuttings into the plug tray, with one cutting per hole, and the cutting depth is 3 cm;

[0035] (3) Cutting management: After the cutting is completed, water thoroughly and spray 1000 times potassium humate once, then cover the hole tray with a shade net, maintain the temperature at 20℃ and the humidity at 80%, spray 0.1% carbendazim solution once a week, and transplant after 70 days of cultivation.

[0036] Embodiment 2:

[0037] S1: Add benzoic acid to water, heat and stir until completely dissolved to obtain a 5wt% benzoic acid solution; mix 22.5g of benzoic acid solution, 3.5g of palm oil, 2.25g of rosin glycerol ester and 22g of volcanic mud, stir at 65°C for 45min, and cool to obtain modified volcanic mud;

[0038] S2: 1.25 g of gliadin was added to a 70 wt % ethanol solution and stirred until completely dissolved, and then water was added to obtain a 2 wt % gliadin solution, and then 1.1 g of xylan and 1.35 g of sodium laurate were added to the gliadin solution and mixed to obtain a solution A;

[0039] S3: The modified volcanic mud and solution A are mixed evenly in a mass ratio of 22:7, and the water content is adjusted to 42% to obtain a treatment agent.

[0040] Cutting method of Cyclobalanopsis xichouensis:

[0041] (1) Treatment of cuttings: Select semi-lignified Cyclobalanopsis glauca branches with full leaf buds, cut the Cyclobalanopsis glauca branches to a length of 9 cm, retain the top two leaves, and retain 55% of the area of ​​each leaf. The cuttings are cut flat and the lower end is cut obliquely to obtain cuttings. Then, the treatment agent is applied to the cutting of the cuttings. The amount used is 12 g / plant, and then the cuttings are left to stand for 35 minutes. After the treatment is completed, the treatment agent at the cutting of the cuttings is gently tapped to remove the treatment agent, thereby obtaining treated cuttings;

[0042] (2) Cutting: Mix perlite and peat soil in a volume ratio of 1:2 to obtain a cutting medium, then fill the plug tray with the cutting medium and compact it slightly, and disinfect the plug tray with 1% potassium permanganate solution, then insert the treated cuttings into the plug tray, with one cutting per hole, and the cutting depth is 3 cm;

[0043] (3) Cutting management: After the cutting is completed, water thoroughly and spray 1000 times potassium humate once, then cover the hole tray with a shade net, maintain the temperature at 22°C and the humidity at 82%, spray 0.1% carbendazim solution once a week, and transplant after 75 days of cultivation.

[0044] Embodiment 3:

[0045] S1: Add benzoic acid to water, heat and stir until completely dissolved to obtain a 5wt% benzoic acid solution; evenly mix 25g of benzoic acid solution, 4g of palm oil, 2.5g of rosin glycerol ester and 20g of volcanic mud, stir at 80°C for 50min, and cool to obtain modified volcanic mud;

[0046] S2: 1.5 g of gliadin was added to a 70 wt % ethanol solution and stirred until completely dissolved, and then water was added to obtain a 2 wt % gliadin solution, and then 1.2 g of xylan and 1.5 g of sodium laurate were added to the gliadin solution and mixed to obtain a solution A;

[0047] S3: The modified volcanic mud and solution A are mixed evenly in a mass ratio of 25:8, and the water content is adjusted to 45% to obtain a treatment agent.

[0048] Cutting method of Cyclobalanopsis xichouensis:

[0049] (1) Treatment of cuttings: Select semi-lignified Cyclobalanopsis glauca branches with full leaf buds, cut the branches to a length of 10 cm, retain the top three leaves, and retain 60% of the area of ​​each leaf. The cuttings are cut flat and the lower end is cut obliquely to obtain cuttings. Then, the treatment agent is applied to the cuttings at an amount of 15 g / plant, and then left to stand for 40 minutes. After the treatment is completed, the treatment agent at the cuttings is gently tapped to remove the treatment agent, thereby obtaining treated cuttings;

[0050] (2) Cutting: Mix perlite and peat soil in a volume ratio of 1:2 to obtain a cutting medium, then fill the plug tray with the cutting medium and compact it slightly, and disinfect the plug tray with 1% potassium permanganate solution, then insert the treated cuttings into the plug tray, with one cutting per hole, and the cutting depth is 4 cm;

[0051] (3) Cutting management: After the cutting is completed, water thoroughly and spray 1000 times potassium humate once, then cover the hole tray with a shade net, maintain the temperature at 25°C and the humidity at 85%, spray 0.1% carbendazim solution once a week, and transplant after 80 days of cultivation.

[0052] Comparative Example 1:

[0053] This comparative example is in contrast to Example 1, the only difference being the method for preparing the treatment agent, that is, the volcanic mud is not treated, and the specific method is as follows:

[0054] S1: 1.2 g of gliadin was added to a 70 wt % ethanol solution and stirred until completely dissolved, and then water was added to obtain a 2 wt % gliadin solution, and then 1 g of xylan and 1.2 g of sodium laurate were added to the gliadin solution and mixed to obtain a solution A;

[0055] S2: The modified volcanic mud and solution A are mixed evenly in a mass ratio of 20:6, and the water content is adjusted to 40% to obtain a treatment agent.

[0056] The cutting method of Cyclobalanopsis glauca in this comparative example is the same as that in Example 1.

[0057] Comparative Example 2:

[0058] This comparative example is in contrast to Example 1, the only difference being the raw material of the treatment agent, specifically, no benzoic acid is added, and the specific method is as follows:

[0059] S1: 3 g palm oil, 2 g rosin glycerol ester and 20 g volcanic mud were mixed evenly, stirred at 50 ° C for 40 min, and cooled to obtain modified volcanic mud;

[0060] S2: 1.2 g of gliadin was added to a 70 wt % ethanol solution and stirred until completely dissolved, and then water was added to obtain a 2 wt % gliadin solution, and then 1 g of xylan and 1.2 g of sodium laurate were added to the gliadin solution and mixed to obtain a solution A;

[0061] S3: The modified volcanic mud and solution A are mixed evenly in a mass ratio of 20:6, and the water content is adjusted to 40% to obtain a treatment agent.

[0062] The cutting method of Cyclobalanopsis glauca in this comparative example is the same as that in Example 1.

[0063] Comparative Example 3:

[0064] This comparative example is in contrast to Example 1, the only difference being the raw material of the treatment agent, specifically, no palm oil is added, and the specific method is as follows:

[0065] S1: Add benzoic acid to water, heat and stir until completely dissolved to obtain a 5wt% benzoic acid solution; mix 20g of the benzoic acid solution, 2g of rosin glycerol ester and 20g of volcanic mud, stir at 50°C for 40min, and cool to obtain modified volcanic mud;

[0066] S2: 1.2 g of gliadin was added to a 70 wt % ethanol solution and stirred until completely dissolved, and then water was added to obtain a 2 wt % gliadin solution, and then 1 g of xylan and 1.2 g of sodium laurate were added to the gliadin solution and mixed to obtain a solution A;

[0067] S3: The modified volcanic mud and solution A are mixed evenly in a mass ratio of 20:6, and the water content is adjusted to 40% to obtain a treatment agent.

[0068] The cutting method of Cyclobalanopsis glauca in this comparative example is the same as that in Example 1.

[0069] Comparative Example 4:

[0070] This comparative example is in contrast to Example 1, the only difference being that the treating agent is prepared as a solution, that is, no rosin glycerol ester is added. The specific method is as follows:

[0071] S1: Add benzoic acid to water, heat and stir until completely dissolved to obtain a 5wt% benzoic acid solution; mix 20g of the benzoic acid solution, 3g of palm oil and 20g of volcanic mud, stir at 50°C for 40min, and cool to obtain modified volcanic mud;

[0072] S2: 1.2 g of gliadin was added to a 70 wt % ethanol solution and stirred until completely dissolved, and then water was added to obtain a 2 wt % gliadin solution, and then 1 g of xylan and 1.2 g of sodium laurate were added to the gliadin solution and mixed to obtain a solution A;

[0073] S3: The modified volcanic mud and solution A are mixed evenly in a mass ratio of 20:6, and the water content is adjusted to 40% to obtain a treatment agent.

[0074] The cutting method of Cyclobalanopsis glauca in this comparative example is the same as that in Example 1.

[0075] Comparative Example 5:

[0076] This comparative example is in contrast to Example 1, the only difference being the raw materials of the treatment agent, specifically, no gliadin is added, and the specific method is as follows:

[0077] S1: Add benzoic acid to water, heat and stir until completely dissolved to obtain a 5wt% benzoic acid solution; mix 20g of benzoic acid solution, 3g of palm oil, 2g of rosin glycerol ester and 20g of volcanic mud, stir at 50°C for 40min, and cool to obtain modified volcanic mud;

[0078] S2: Add 1 g of xylan and 1.2 g of sodium laurate to 60 ml of water and mix well to obtain solution A;

[0079] S3: The modified volcanic mud and solution A are mixed evenly in a mass ratio of 20:6, and the water content is adjusted to 40% to obtain a treatment agent.

[0080] The cutting method of Cyclobalanopsis glauca in this comparative example is the same as that in Example 1.

[0081] Comparative Example 6:

[0082] This comparative example is in contrast to Example 1, the only difference being the raw material of the treatment agent, specifically, no xylan is added, and the specific method is as follows:

[0083] S1: Add benzoic acid to water, heat and stir until completely dissolved to obtain a 5wt% benzoic acid solution; mix 20g of benzoic acid solution, 3g of palm oil, 2g of rosin glycerol ester and 20g of volcanic mud, stir at 50°C for 40min, and cool to obtain modified volcanic mud;

[0084] S2: adding 1.2 g of gliadin to a 70 wt % ethanol solution and stirring until completely dissolved, then adding water to obtain a 2 wt % gliadin solution, then adding 1.2 g of sodium laurate to the gliadin solution, and mixing well to obtain a solution A;

[0085] S3: The modified volcanic mud and solution A are mixed evenly in a mass ratio of 20:6, and the water content is adjusted to 40% to obtain a treatment agent.

[0086] The cutting method of Cyclobalanopsis glauca in this comparative example is the same as that in Example 1.

[0087] Comparative Example 7:

[0088] This comparative example is in contrast to Example 1, and the difference lies only in the different cutting process, specifically, using a treatment solution obtained by mixing gliadin and xylan to treat the cuttings, and the specific method is as follows:

[0089] 1.2 g of gliadin was added to a 70 wt % ethanol solution and stirred until completely dissolved, and then water was added to obtain a 2 wt % gliadin solution. Then 1 g of xylan was added to the gliadin solution and mixed evenly to obtain a treated solution.

[0090] (1) Treatment of cuttings: Select semi-lignified Cyclobalanopsis glauca branches with full leaf buds, cut the branches to a length of 8 cm, retain the top two leaves, and retain 50% of the area of ​​each leaf. The cuttings are cut flat on the top and beveled on the bottom to obtain cuttings, and then the cuttings are placed in the treatment solution and soaked for 1 hour to obtain treated cuttings;

[0091] (2)-(3) are the same as in Example 1.

[0092] experiment:

[0093] (1) Treatment of cuttings: In early May, semi-lignified Cyclobalanopsis glauca branches with full leaf buds were collected, and the branches were cut to a length of 8 cm, retaining the top two leaves and 50% area of ​​each leaf. The cuttings were cut flat and the lower end was cut obliquely to obtain cuttings, and then the treatment agent was applied to the cutting of the cuttings. The amount used was 10 g / plant, and the treatment was allowed to stand for 30 minutes. After the treatment was completed, the treatment agent at the cutting of the cuttings was gently tapped to remove the treatment agent, thereby obtaining treated cuttings;

[0094] (2) Cutting: Mix perlite and peat soil in a volume ratio of 1:2 to obtain a cutting medium, then fill the plug tray with the cutting medium and compact it slightly, and disinfect the plug tray with 1% potassium permanganate solution, then insert the treated cuttings into the plug tray, with one cutting per hole, and the cutting depth is 3 cm;

[0095] (3) Cutting management: After the cutting is completed, water it thoroughly and spray it with 1000 times potassium humate. Then, cover the hole tray with a shade net, maintain the temperature at 20°C and the humidity at 80%. Spray it with 0.1% carbendazim solution once a week. After 70 days of cultivation, transplant it into a substrate with a mass ratio of perlite to yellow mud of 1:3.

[0096] 1. Experimental group 1 used the treatment agent prepared in Example 1;

[0097] 2. Comparative groups 1-6 used the treatment agents prepared in comparative example groups 1-6 respectively;

[0098] 3. Comparative Group 7 used the treatment solution prepared in Comparative Example 7 and the method of Comparative Example 7 for cuttings;

[0099] 4. Comparative Group 8 used 30 wt % acetone solution to treat cuttings, and the specific steps (1) were as follows:

[0100] Treatment of cuttings: Select semi-lignified and full-leafed Cyclobalanopsis glauca branches, cut the Cyclobalanopsis glauca branches to a length of 8 cm, retain the top two leaves, and retain 50% of the area of ​​each leaf. The cuttings are flat on the cuttings and beveled on the lower ends to obtain the cuttings. Then, 30 wt% of the cuttings are immersed in an acetone solution for 1 hour to obtain the treated cuttings.

[0101] 5. The comparative group 9 used clean water to treat the cuttings. The specific steps (1) were as follows:

[0102] (1) Treatment of cuttings: Select semi-lignified Cyclobalanopsis glauca branches with full leaf buds, cut the branches to a length of 8 cm, retain the top two leaves, and retain 50% of the area of ​​each leaf. The cuttings are cut flat on the top and beveled on the bottom to obtain cuttings, and then soak the cuttings in clean water for 1 hour to obtain treated cuttings;

[0103] Each group was cut with 50 cuttings, repeated three times, and the rooting time of the cuttings was recorded. The results are shown in Table 1. Two months after the cuttings were cut, the rooting rate and survival rate of the cuttings were recorded, and the average values ​​were taken. The results are shown in Table 1.

[0104] Table 1

[0105]

[0106]

[0107] Analyzing Table 1, we can get:

[0108] 1. Compared with experimental group 1, control group 1 did not treat the volcanic mud. The volcanic mud had strong adsorption and absorbed the water of the cuttings during the treatment process, resulting in water loss of the cuttings, decreased rooting rate, and decreased survival rate. In addition, the volcanic mud gradually lost water and solidified and lost its original viscosity, causing the treatment agent to fall off from the cuttings, reducing the removal efficiency of tannins, resulting in a high tannin content at the cutting cut, affecting the rooting of the cuttings. Therefore, the rooting time of the cuttings in control group 1 was longer and the rooting rate was lower. Compared with experimental group 1, control group 2 did not add benzoic acid. The hydrophilicity of the volcanic mud was higher. During the treatment of the cuttings, the volcanic mud absorbed the water of the cuttings, resulting in water loss of the cuttings and reduced rooting ability. Therefore, the rooting time of control group 2 was longer and the rooting rate was lower. In addition, benzoic acid can also promote the rooting of the cuttings, so the further rooting rate of control group 2 without the addition of benzoic acid was reduced. Compared with experimental group 1, control group 3 did not add palm oil, and the rooting rate and survival rate of the cuttings were lower. This is because palm oil can combine with the hydroxyl groups on the surface of volcanic mud to reduce the water absorption capacity of volcanic mud, thereby preventing the cuttings from being absorbed by volcanic mud during the treatment process, resulting in a lower rooting rate. Comparison group 3 did not add palm oil, so the volcanic mud in its treatment agent would absorb the moisture of the cuttings, resulting in a lower rooting ability of the cuttings. Compared with experimental group 1, control group 4 did not add rosin glycerol ester, and the treatment agent had lower adhesion, resulting in the treatment agent easily falling off from the cutting cut after dehydration and drying, thereby reducing the treatment efficiency of tannins. The tannins at the cut affected the rooting of the cuttings, resulting in a longer rooting time and a lower rooting rate.

[0109] 2. Comparative Example 5 does not add gliadin, and Comparative Group 6 does not add xylan. The tannin removal rate of the cuttings treated by the comparative example is low, and the rooting rate and survival rate are also lower than those of Experimental Group 1. This is because the tannins retained on the surface of the cuttings in Comparative Groups 5 and 6 affect the ability of the cuttings to absorb water and nutrients, thereby reducing the rooting ability of the cuttings, and the root system develops relatively slowly, and the rooting time is long, which leads to a low survival rate of the cuttings. Compared with Experimental Group 1, Comparative Group 7 uses a treatment solution obtained by mixing gliadin and xylan to treat the cuttings. The precipitation generated by tannins, gliadin and xylan, respectively, will accumulate in the cuttings, affecting the ability of the cuttings to absorb water and nutrients, thereby leading to a long rooting time of the cuttings, a low rooting rate, and a low survival rate.

[0110] 3. Compared with experimental group 1, comparative group 8 uses acetone solution to treat cuttings. The acetone solution can dissolve the tannins on the surface of the cutting cut into the acetone solution, reducing the effect of tannins on the rooting of the cuttings. However, the tannin removal rate of the cuttings after treatment is lower than that of experimental group 1, and the rooting rate is also lower than that of experimental group 1, indicating that the treatment agent prepared in Example 1 can well remove the tannins at the cutting cut and promote the rooting of the cuttings. Compared with comparative group 8, comparative group 9 uses clean water to treat cuttings, and the dissolution rate of tannins is lower. The tannins in the cuttings cannot be effectively treated, which inhibits the rooting of the cuttings, resulting in low rooting ability of the cuttings and a long rooting time, thereby reducing the survival rate of the cuttings.

[0111] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the purpose and scope of the technical solutions of the present invention, which should be included in the scope of the claims of the present invention. The techniques, shapes, and structural parts not described in detail in the present invention are all known technologies.

Claims

1. A cutting method for Cyclobalanopsis xichouensis, characterized in that: The method comprises the following steps: (1) Treatment of cuttings: Apply a treatment agent to the cutting cut, and allow to stand for 30-40 minutes. After the treatment is completed, remove the treatment agent from the cutting cut to obtain treated cuttings; (2) Cutting: Fill the plug tray with the matrix, disinfect the plug tray with 1% potassium permanganate solution, and then insert the treated cuttings into the plug tray; (3) Cutting management: After the cutting is completed, water thoroughly and spray 1000 times potassium humate once, then cover the hole tray with a shade net to maintain the temperature and humidity, and spray 0.1% carbendazim solution once a week.

2. The cutting method of Cyclobalanopsis xichouensis according to claim 1, characterized in that: The method for processing cuttings in step (1) is as follows: cutting the Cyclobalanopsis glauca branches to a length of 8-10 cm, retaining 2-3 leaves at the top and 50-60% of the area of ​​each leaf, making a flat cut on the cuttings and an oblique cut at the bottom close to the leaf nodes.

3. The cutting method of Cyclobalanopsis xichouensis according to claim 2, characterized in that: In step (3), the temperature is maintained at 20-25° C. and the humidity is maintained at 80-85%.

4. The cutting method of Cyclobalanopsis xichouensis according to claim 3, characterized in that: The preparation method of the treatment agent is as follows: S1: adding benzoic acid to water, heating and stirring until completely dissolved to obtain a 5wt% benzoic acid solution; mixing the benzoic acid solution, palm oil, rosin glycerol ester and volcanic mud evenly, stirring at 50°C-80°C for 40-50min, and cooling to obtain modified volcanic mud; S2: adding gliadin to a 70 wt % ethanol solution, stirring evenly, adding water to prepare a 2 wt % gliadin solution, and then adding xylan and sodium laurate to the gliadin solution, mixing evenly to obtain solution A; S3: The modified volcanic mud and solution A are mixed and stirred evenly, and the water content is adjusted to 40-45% to obtain a treatment agent.

5. The cutting method of Cyclobalanopsis xichouensis according to claim 4, characterized in that: In step S1 of the method for preparing the treatment agent, the mass ratio of benzoic acid solution, palm oil, rosin glycerol ester and volcanic mud is (20-25): (3-4): (2-2.5): (20-25).

6. The cutting method of Cyclobalanopsis xichouensis according to claim 5, characterized in that: In step S2 of the method for preparing the treating agent, the mass ratio of gliadin, xylan and sodium laurate is (1-1.5): (1-1.2): (1.2-1.5).

7. The cutting method of Cyclobalanopsis xichouensis according to claim 6, characterized in that: In step S3 of the method for preparing the treatment agent, the mass ratio of modified volcanic mud to solution A is (20-25): (6-8).

8. The cutting method of Cyclobalanopsis xichouensis according to claim 7, characterized in that: The amount of the treatment agent used is 10-15 g / plant.

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