Method for producing natural pearls of pinctada fucata

Abstract

The invention discloses a method for producing natural pearls of pinctada fucata martensii, which comprises the following steps of: S1, preparing pearl growth promoting liquid: dissolving hyaluronic acid with the molecular weight of 1500000-2500000 into phosphate buffer solution with the pH value of 7.2-7.6 to obtain the pearl growth promoting liquid with the concentration of 1000mu g / mL; s2, injecting pearl growth promoting liquid: injecting (100 + / -10) mu L of the obtained pearl growth promoting liquid into pinctada fucata adductor muscle tissues through a 1mL microsyringe; and S3, culturing pearls: putting the injected pinctada fucata bells in a seawater environment with the water temperature of 25 + / -2 DEG C and the salinity of 28 + / -2 per thousand for culturing for 30-60 days. According to the method, hyaluronic acid is creatively used as the pearl growth promoting liquid to be applied to the seawater pearl culture production process, a brand new solution is provided for efficient output of natural pearls, and development of the seawater pearl culture industry is powerfully promoted.

Description

Technical Field

[0001] The invention belongs to the technical field of seawater pearl culture, relates to a method for producing natural pearls of Pinctada fucata, and specifically to a method for promoting the formation of natural pearls of Pinctada fucata by injecting a hyaluronic acid solution. Background Art

[0002] Pearls are divided into natural pearls and cultured pearls. The birth of natural pearls is accidental. When tiny foreign objects such as sand and parasites from the outside world accidentally invade the soft tissues of shellfish, the stimulated mantle epidermal cells respond quickly. They gradually sink into the deep connective tissue of the mantle with the foreign objects as the center. The mantle epidermal cells that are trapped in it continue to divide themselves, wrap around the foreign objects layer by layer, and gradually form a pearl sac with the foreign objects as the core. The production of cultured pearls requires the help of human intervention to accurately implant the mantle tissue and the bead nucleus into the shellfish. The mantle tissue of the shellfish continues to proliferate around the artificial bead nucleus, gradually forming a pearl sac. Subsequently, the pearl sac secretes nacre, undergoes a series of complex biological processes, and finally realizes the artificial cultivation of pearls.

[0003] However, whether it is the natural formation of natural pearls or the artificial creation of artificial pearl cultivation, the bead nucleus is always a vital and indispensable core element. The formation of natural pearls depends on the accidental intrusion of foreign objects. This uncertainty leads to extremely limited production of natural pearls. How to transform this elusive "accident" into controllable cultivation conditions has become a core problem that needs to be overcome in the field of natural pearl cultivation. In the process of artificial pearl cultivation, the artificially implanted bead nucleus has a strong stimulation on the physiological functions of shellfish, which not only threatens the healthy survival of shellfish, but also becomes one of the key constraints hindering the large-scale development of the artificial pearl cultivation industry. Therefore, the search for an ideal material that can efficiently stimulate pearl formation and have little effect on the physiological functions of shellfish has become the current research focus in the field of pearl cultivation.

[0004] At present, there are related studies, such as CN112655615A, which discloses a method of using calcium carbonate powder to stimulate the cultivation of natural seawater pearls. This method is to load calcium carbonate powder into a dry powder sprayer, and then spray calcium carbonate powder into the pearl oyster, so as to promote the pearl oyster to form a pearl sac and then form a pearl. However, this solution has obvious disadvantages, the operation process is complicated and cumbersome, and professional dry powder spraying equipment is required. It also requires operators to have rich experience and professional skills, which makes it difficult to promote and apply in actual seawater pearl farming production.

[0005] Another example is CN114287368A, which discloses a method for cultivating natural seawater pearls. In actual operation, the recipient shellfish needs to be drilled to implant shell powder. This operation process is extremely complicated, requiring not only professional drilling equipment but also experienced operators. Moreover, the entire operation process is time-consuming and greatly increases the cultivation cost. What is more serious is that the drilling operation will cause unignorable damage to the physiological structure of the recipient shellfish, which not only disrupts the normal physiological function of the shellfish, but also weakens its immunity, significantly increases the risk of disease infection, and seriously affects the cultivation success rate and pearl quality.

[0006] In summary, looking at the current technical status of pearl cultivation, although the artificial nucleus insertion pearl cultivation technology is relatively mature and various improvement methods are emerging, mature and efficient natural seawater pearl cultivation methods are still missing. The existing technical solutions, whether it is by spraying calcium carbonate powder for stimulation or by drilling and implanting shell powder, have many disadvantages such as complex operation, high cost, and damage to the physiological functions of shellfish, which are difficult to meet the needs of large-scale, high-quality cultivation of natural seawater pearls. Therefore, it is urgent to develop a safe, efficient, and green method for cultivating natural seawater pearls. Summary of the invention

[0007] In view of the problems in the prior art of using calcium carbonate powder for stimulation or by drilling to implant shell powder, which are complicated in operation, high in cost, and harmful to the physiological functions of shellfish, the present invention provides a method for producing natural pearls from Hepu pearl oysters. The core goal of the present invention is to solve the difficulties in the process of natural pearl production, especially to improve its controllability and efficiency. To this end, the present invention proposes an innovative method, which aims to promote the production of natural seawater pearls by applying pearl growth-promoting liquid to seawater pearl culture production. The implementation of this method is expected to transform the production of natural pearls into an operational process, thereby significantly improving the efficiency of pearl culture and the quality of pearls.

[0008] In order to achieve the above-mentioned invention object, the technical solution adopted by the present invention is:

[0009] A method for producing natural pearls from Hepu mother-of-pearl, comprising the following steps:

[0010] S1. Prepare pearl growth-promoting solution: dissolve hyaluronic acid with a molecular weight of 1.5 million to 2.5 million in phosphate buffered saline (PBS) at pH 7.2 to 7.6 to obtain a pearl growth-promoting solution with a concentration of 1000 μg / mL;

[0011] S2, injection of pearl growth-promoting solution: inject (100±10) μL of the solution obtained in S1 into the adductor muscle tissue of the Hepu Pinctada fucata through a 1 mL microsyringe;

[0012] S3. Pearl cultivation: The injected Pinctada fucata oysters are placed in a seawater environment with a water temperature of (25±2)°C and a salinity of (28±2)‰ for 30 to 60 days.

[0013] Furthermore, in S1, hyaluronic acid with a molecular weight of 1.5 million to 2.5 million is dissolved in a phosphate buffer at pH 7.5.

[0014] Furthermore, in S3, the culture period was 59 days.

[0015] Compared with the prior art, the present invention has the following advantages:

[0016] 1. The method for producing natural pearls from Pinctada pupu described in the present invention innovatively adopts a unique method of injecting hyaluronic acid solution to successfully induce the formation of natural pearls. This breakthrough effectively overcomes the technical bottleneck that has long been faced by artificially culturing natural seawater pearls and fills the key technical gap in this field.

[0017] 2. The method for producing natural pearls from Hepu pearl oysters described in the present invention has significant practical advantages. The operation process is simple and easy, and does not require complicated equipment and professional skills. It has achieved remarkable results in actual applications. The cultivated pearls are of excellent quality and stable yield, showing extremely high application value and promotion potential. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a distribution diagram of natural pearls formed in the Pinctada pupuensis after hyaluronic acid is injected in the method for producing natural pearls in the Pinctada pupuensis according to Example 1 of the present invention;

[0019] Figure 2 This is a distribution diagram of natural pearls formed in the Pinctada pupuensis after hyaluronic acid is injected in the method for producing natural pearls in the Pinctada pupuensis according to Example 1 of the present invention;

[0020] Figure 3 This is a distribution diagram of natural pearls formed in the Pinctada pupuensis after hyaluronic acid is injected in the method for producing natural pearls in the Pinctada pupuensis according to Example 1 of the present invention;

[0021] Figure 4 This is a distribution diagram of natural pearls formed in the Pinctada pupuensis after hyaluronic acid is injected in the method for producing natural pearls in the Pinctada pupuensis according to Example 1 of the present invention;

[0022] Figure 5 This is a surface structure diagram of a natural pearl collected after adding hyaluronic acid in the method for producing a natural pearl from a Hepu mother-of-pearl according to Example 1 of the present invention;

[0023] Figure 6This is a surface structure diagram of a natural pearl collected after adding hyaluronic acid in the method for producing natural pearls from Hepu mother-of-pearl as described in Example 1 of the present invention. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments and drawings. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0025] Embodiment 1:

[0026] Date: August 16, 2024

[0027] Location: Yishun Pearl Farming Factory, Fangchenggang City, Guangxi Zhuang Autonomous Region

[0028] A method for producing natural pearls from the Hepu pearl oyster, using the Hepu pearl oyster as a receptor oyster, comprising the following steps:

[0029] S1. Prepare pearl growth-promoting solution: dissolve hyaluronic acid with a molecular weight of 1.5-2.5 million in phosphate buffered saline (PBS) at pH 7.5 to obtain a pearl growth-promoting solution with a concentration of 1000 μg / mL;

[0030] S2, injecting pearl growth-promoting solution: inject 100±10 μL of the solution obtained in step S1 into the adductor muscle tissue of the Hepu pearl oyster through a 1 mL microsyringe;

[0031] S3. Pearl cultivation: The injected shellfish were placed in a seawater environment with a water temperature of 25±2°C and a salinity of 28±2‰ for 59 days.

[0032] Figure 1 This is a distribution diagram of natural pearls formed in the Hepu mother-of-pearl after hyaluronic acid is injected in the Hepu mother-of-pearl natural pearl production method described in Example 1;

[0033] Figure 2 This is a distribution diagram of natural pearls formed in the Hepu mother-of-pearl after hyaluronic acid is injected in the Hepu mother-of-pearl natural pearl production method described in Example 1;

[0034] Figure 3 This is a distribution diagram of natural pearls formed in the Hepu mother-of-pearl after hyaluronic acid is injected in the Hepu mother-of-pearl natural pearl production method described in Example 1;

[0035] Figure 4 This is a distribution diagram of natural pearls formed in the Hepu mother-of-pearl after hyaluronic acid is injected in the Hepu mother-of-pearl natural pearl production method described in Example 1;

[0036] Figure 5 This is a surface structure diagram of a natural pearl collected after adding hyaluronic acid in the method for producing a natural pearl from a Hepu mother-of-pearl described in Example 1;

[0037] Figure 6 This is a surface structure diagram of a natural pearl collected after adding hyaluronic acid in the production method of a natural pearl from a Hepu mother-of-pearl as described in Example 1.

[0038] Embodiment 2:

[0039] Date: August 25, 2024

[0040] Location: Yishun Pearl Farming Factory, Fangchenggang City, Guangxi Zhuang Autonomous Region

[0041] A method for producing natural pearls from the Hepu pearl oyster, using the Hepu pearl oyster as a receptor oyster, comprising the following steps:

[0042] S1. Prepare pearl growth-promoting solution: dissolve hyaluronic acid with a molecular weight of 1.5-2.5 million in phosphate buffered saline (PBS) at pH 7.2 to obtain a pearl growth-promoting solution with a concentration of 1000 μg / mL;

[0043] S2, injecting pearl growth-promoting solution: inject 100±10 μL of the solution obtained in step S1 into the adductor muscle tissue of the Hepu pearl oyster through a 1 mL microsyringe;

[0044] S3. Pearl cultivation: The injected shellfish are placed in a seawater environment with a water temperature of 25±2°C and a salinity of 28±2‰ for 30 days.

[0045] Comparative Example:

[0046] The difference from Example 1 is that only PBS is injected in S1, and the other steps are the same as Example 1.

[0047] Experimental Design:

[0048] 150 pearls were implanted in the hyaluronic acid injection group (Example 1, hyaluronic acid dissolved in PBS) and the control group (comparative example, PBS injection), and the small piece preparation and implantation were performed by the same group of personnel. The pearl cultivation period lasted for 59 days.

[0049] result:

[0050] The results of the analysis of the samples of Hepu pearl oysters 59 days after the injection of hyaluronic acid showed that no natural pearl formation was observed in the control group (PBS injection) during sampling; while the experimental group (hyaluronic acid injection group) successfully induced natural pearls ( Figure 1-Figure 4 ).

[0051] The above description is a detailed description of the preferred feasible embodiments of the present invention, but the embodiments are not intended to limit the scope of the patent application of the present invention. All equivalent changes or modified changes completed under the technical spirit suggested by the present invention should fall within the patent scope covered by the present invention.

Claims

1. A method for producing natural pearls from Hepu mother-of-pearl, characterized in that: The steps include: S1. Prepare pearl growth-promoting solution: dissolve hyaluronic acid with a molecular weight of 1.5 million to 2.5 million in a phosphate buffer with a pH of 7.2 to 7.6 to obtain a pearl growth-promoting solution with a concentration of 1000 μg / mL; S2, injection of pearl growth-promoting solution: inject (100±10) μL of the solution obtained in S1 into the adductor muscle tissue of the Hepu Pinctada fucata through a 1 mL microsyringe; S3. Pearl cultivation: The injected Pinctada fucata oysters are placed in a seawater environment with a water temperature of (25±2)°C and a salinity of (28±2)‰ for 30 to 60 days.

2. The method for producing natural pearls from Pinctada puerta according to claim 1, characterized in that: In S1, hyaluronic acid with a molecular weight of 1.5 million to 2.5 million is dissolved in a phosphate buffer at pH 7.

5.

3. The method for producing natural pearls from Pinctada pupuensis according to claim 1, characterized in that: In S3, the culture period is 59 days.

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