Composite agent for preventing and controlling pine tree trunk borers and use method of composite agent

Abstract

The invention relates to the technical field of tree protection, and particularly discloses a composite agent for preventing and controlling pine trunk borers and a use method of the composite agent. The invention discloses a composite medicament for preventing and controlling pine trunk borers. The composite medicament comprises a component A and a component B, the component A is prepared from the following raw materials: emamectin benzoate and an emulsifier; the component B is prepared from the following raw materials: an emulsifying agent, an adhesive, cypermethrin and emamectin benzoate. According to the application, the component A consisting of the emamectin benzoate and the emulsifier and the component B consisting of the emulsifier, the adhesive, the cypermethrin and the emamectin benzoate are matched for use, so that the prevention and control effect on the monochamus alternatus and the monochamus alternatus is improved, and the healthy growth of pine wood is promoted.

Description

Technical Field

[0001] The present application relates to the technical field of tree protection, and in particular to a composite agent for preventing and controlling pine trunk-boring pests and a method of using the composite agent. Background Art

[0002] Pine knot weevils and pine brown alternator are the main trunk pests of pine trees, belonging to the order Coleoptera. Pine knot weevils are distributed in Jiangsu, Fujian, Jiangxi, Hunan and other places in my country. They mainly harm large-diameter Masson pine timber and concentrate their damage in Masson pine forests. The affected trees may die in 1-2 months, with up to 15 larvae in the trees, causing the trees to weaken or even die. Pine brown alternator is widely distributed in pine forests and is the main vector of pine wilt disease. Severe outbreaks of pine brown alternator cause the disease to spread rapidly. These two trunk pests can continue to bore during the storage period of logs, so we must be highly vigilant and take active prevention and control measures. Commonly used pesticides have poor control effects on pine knot weevils and pine brown alternator, which will seriously affect the healthy growth of pine timber. Summary of the invention

[0003] In order to improve the control effect on pine gall weevils and pine brown alternator beetles, the present application provides a composite agent for controlling pine trunk borers and a method of using the same.

[0004] In the first aspect, the present application provides a composite agent for controlling pine trunk borers, which adopts the following technical scheme: a composite agent for controlling pine trunk borers, comprising component A and component B; the raw materials of component A include avermectin benzoate and an emulsifier; the raw materials of component B include an emulsifier, an adhesive, cypermethrin, and avermectin benzoate.

[0005] By adopting the above technical scheme, component A consisting of avermectin benzoate and emulsifier, and component B consisting of emulsifier, adhesive, cypermethrin and avermectin benzoate are used in combination, thereby improving the prevention and control effect on pine gall weevils and pine brown alternator, thereby promoting the healthy growth of pine wood.

[0006] In a specific embodiment, the volume ratio of avermectin benzoate to emulsifier in component A is (8-10):0.2.

[0007] In a specific embodiment, the emulsifier includes emulsifier OP-10.

[0008] By adopting the above technical scheme, the types of emulsifiers and the ratio with avermectin benzoate are further limited, thereby improving the control effect of component A on pine gall weevils and pine brown alternator.

[0009] In a specific embodiment, the volume ratio of the emulsifier, adhesive, cypermethrin and emamectin benzoate in component B is 0.4:(0.4-0.6):(7.5-8.5):(2-5).

[0010] By adopting the above technical scheme, the ratio of emulsifier, adhesive, cypermethrin and avermectin benzoate in component B is further limited, thereby further improving the control effect of component B on pine gall weevils and pine brown alternator.

[0011] In a specific embodiment, the cypermethrin is pretreated by the following steps: Dissolve cypermethrin in dichloromethane, then add a sustained-release agent and a stabilizer, stir and dissolve, and obtain a mixed solution A; dissolving poly(lactic acid-co-glycolic acid) copolymer in dichloromethane to form a mixed solution B; The mixed solution A and the mixed solution B are mixed, and a polysorbate-20 solution is added to obtain a mixed solution C. The mixed solution C is stirred and sheared to obtain an emulsion, and dichloromethane is removed by rotary evaporation at 40° C. to obtain a suspension, which is filtered, and the filter residue is washed and dried to obtain cypermethrin microcapsules.

[0012] By adopting the above technical solution, cypermethrin microcapsules can sustain release and prolong drug efficacy, improve bioavailability, reduce environmental pollution and enhance safety.

[0013] In a specific embodiment, the weight ratio of cypermethrin, dichloromethane, sustained-release agent and stabilizer in the mixed solution A is (0.3-0.32):9:(0.45-0.47):(0.4-0.6); the weight ratio of polylactic acid-glycolic acid copolymer and dichloromethane in the mixed solution B is (0.92-0.94):10; the weight ratio of mixed solution A, mixed solution B and polysorbate-20 solution in the mixed solution C is 1:(1.06-1.07):(1.92-1.94).

[0014] By adopting the above technical scheme, the ratio of each raw material in the mixed solution A and the mixed solution B, as well as the ratio of the mixed solution A, the mixed solution B and the polysorbate-20 solution are further limited, so that cypermethrin microcapsules can be better prepared.

[0015] In a specific embodiment, the adhesive comprises polyethylene glycol.

[0016] In the second aspect, the present application provides a method for using a composite agent for controlling pine trunk borers, which adopts the following technical solution: A method for using a composite agent for preventing and controlling pine trunk borers comprises the following steps: Use of component A: Add emamectin benzoate and emulsifier into water, stir and mix evenly to obtain component A; in November and December, drill a hole at a depth of 5 cm to 8 cm at a downward angle of 45 degrees with an electric drill of about 1 cm at the base of the tree trunk 5 cm below the ground, and then inject component A; Use of component B: add emulsifier, adhesive, cypermethrin and avermectin benzoate into water, stir and mix evenly to obtain component B; in mid-May and mid-to-late June of the following year, use a drone to spray component B once in the forest area where component A has been injected on sunny evenings or early mornings.

[0017] By adopting the above technical solution, component A is first injected into a hole near the base of the tree trunk, and then component B is sprayed the following year, so that pine knot weevils and pine brown alternator beetles can be effectively prevented and controlled.

[0018] In a specific feasible implementation plan, when component A is used, the number of holes is determined according to the tree's diameter at breast height. When the tree's diameter at breast height is less than 20 cm, the number of holes is 2. When the tree's diameter at breast height is greater than 20 cm, the number of holes increases by 1 for every increase of 10 cm, and also increases by 1 for less than 10 cm. The deep holes are symmetrically distributed, and the injection volume is 20 ml. When component B is used, the application rate is 5 L / mu.

[0019] By adopting the above technical scheme, the usage of component A and component B is further limited, thereby further improving the prevention and control effect on pine knot weevils and pine brown alternating beetles.

[0020] In summary, the present application includes at least one of the following beneficial technical effects: 1. In the present application, component A consisting of avermectin benzoate and an emulsifier, and component B consisting of an emulsifier, an adhesive, cypermethrin, and avermectin benzoate are used in combination to improve the control effect of pine gall weevils and pine brown beetles, thereby promoting the healthy growth of pine wood; 2. In the present application, cypermethrin is pretreated to obtain cypermethrin microcapsules, which can slow release and prolong the efficacy, improve bioavailability, reduce environmental pollution and enhance safety; 3. The method of use in this application is to first drill a hole near the base of the tree trunk and inject component A, and then spray component B the following year, so as to effectively prevent and control pine knot weevils and pine brown alternator beetles. DETAILED DESCRIPTION

[0021] The present application is further described in detail below with reference to the embodiments.

[0022] All raw materials in the examples are commercially available.

[0023] Preparation Example Preparation Example 1 Preparation Example 1 provides a method for pretreatment of cypermethrin, comprising the following steps: Dissolve cypermethrin in dichloromethane, then add a sustained-release agent and a stabilizer, stir and dissolve, and obtain a mixed solution A; wherein the weight ratio of cypermethrin, dichloromethane, sustained-release agent, and stabilizer in the mixed solution A is 0.3:9:0.45:0.4; the sustained-release agent is ethyl cellulose; and the stabilizer is vitamin E; Dissolving polylactic acid-glycolic acid copolymer in dichloromethane to form a mixed solution B; wherein the weight ratio of polylactic acid-glycolic acid copolymer to dichloromethane in the mixed solution B is 0.92:10; Mixed solution A and mixed solution B are mixed, polysorbate-20 solution is added to obtain mixed solution C, mixed solution C is stirred and sheared at a rate of 12000 r / min to obtain an emulsion, dichloromethane is removed by rotary evaporation at 40°C to obtain a suspension, filtered, the filter residue is washed, and dried to obtain cypermethrin microcapsules; wherein the weight ratio of mixed solution A, mixed solution B, and polysorbate-20 solution in mixed solution C is 1:1.06:1.92; the polysorbate-20 solution is a mixed solution of polysorbate-20 and deionized water, and the weight fraction of polysorbate-20 is 10%.

[0024] Preparation Example 2 Preparation Example 2 provides a method for pretreatment of cypermethrin, comprising the following steps: Dissolve cypermethrin in dichloromethane, then add a sustained-release agent and a stabilizer, stir and dissolve, and obtain a mixed solution A; wherein the weight ratio of cypermethrin, dichloromethane, sustained-release agent, and stabilizer in the mixed solution A is 0.31:9:0.46:0.5; the sustained-release agent is ethyl cellulose; and the stabilizer is vitamin E; Dissolving polylactic acid-glycolic acid copolymer in dichloromethane to form a mixed solution B; wherein the weight ratio of polylactic acid-glycolic acid copolymer to dichloromethane in the mixed solution B is 0.93:10; Mixed solution A and mixed solution B are mixed, polysorbate-20 solution is added to obtain mixed solution C, mixed solution C is stirred and sheared at a rate of 12000 r / min to obtain an emulsion, dichloromethane is removed by rotary evaporation at 40°C to obtain a suspension, filtered, the filter residue is washed, and dried to obtain cypermethrin microcapsules; wherein the weight ratio of mixed solution A, mixed solution B, and polysorbate-20 solution in mixed solution C is 1:1.065:1.93; the polysorbate-20 solution is a mixed solution of polysorbate-20 and deionized water, and the weight fraction of polysorbate-20 is 10%.

[0025] Preparation Example 3 Preparation Example 3 provides a method for pretreatment of cypermethrin, comprising the following steps: Dissolve cypermethrin in dichloromethane, then add a sustained-release agent and a stabilizer, stir and dissolve, and obtain a mixed solution A; wherein the weight ratio of cypermethrin, dichloromethane, sustained-release agent, and stabilizer in the mixed solution A is 0.32:9:0.47:0.6; the sustained-release agent is ethyl cellulose; and the stabilizer is vitamin E; Dissolving polylactic acid-glycolic acid copolymer in dichloromethane to form a mixed solution B; wherein the weight ratio of polylactic acid-glycolic acid copolymer to dichloromethane in the mixed solution B is 0.94:10; Mixed solution A and mixed solution B are mixed, polysorbate-20 solution is added to obtain mixed solution C, mixed solution C is stirred and sheared at a rate of 12000 r / min to obtain an emulsion, dichloromethane is removed by rotary evaporation at 40°C to obtain a suspension, filtered, the filter residue is washed, and dried to obtain cypermethrin microcapsules; wherein the weight ratio of mixed solution A, mixed solution B, and polysorbate-20 solution in mixed solution C is 1:1.07:1.94; the polysorbate-20 solution is a mixed solution of polysorbate-20 and deionized water, and the weight fraction of polysorbate-20 is 10%. Example

[0026] Example 1 Example 1 provides a method for preparing a composite agent for controlling pine trunk borers, comprising the following steps: Add emamectin benzoate and emulsifier into water, stir and mix evenly to obtain component A; wherein the volume ratio of emamectin benzoate, emulsifier and water is 8:0.2:91.8; the emulsifier is emulsifier OP-10; Add an emulsifier, an adhesive, cypermethrin and emamectin benzoate into water, stir and mix evenly to obtain component B; wherein the volume ratio of the emulsifier, the adhesive, cypermethrin, emamectin benzoate and water is 0.4:0.4:7.5:2:89.7; wherein the emulsifier is emulsifier OP-10; the adhesive is polyethylene glycol; and the cypermethrin is the cypermethrin microcapsule in Preparation Example 1.

[0027] Example 2 Embodiment 2 provides a method for preparing a composite agent for controlling pine trunk borers, comprising the following steps: Add emamectin benzoate and emulsifier into water, stir and mix evenly to obtain component A; wherein the volume ratio of emamectin benzoate, emulsifier and water is 9:0.2:90.8; the emulsifier is emulsifier OP-10; Add an emulsifier, an adhesive, cypermethrin and emamectin benzoate into water, stir and mix evenly to obtain component B; wherein the volume ratio of the emulsifier, the adhesive, cypermethrin, emamectin benzoate and water is 0.4:0.5:8:3.5:87.6; wherein the emulsifier is emulsifier OP-10; the adhesive is polyethylene glycol; and the cypermethrin is the cypermethrin microcapsule in Preparation Example 1.

[0028] Example 3 Example 3 provides a method for preparing a composite agent for controlling pine trunk borers, comprising the following steps: Add emamectin benzoate and emulsifier into water, stir and mix evenly to obtain component A; wherein the volume ratio of emamectin benzoate, emulsifier and water is 10:0.2:89.8; the emulsifier is emulsifier OP-10; Add an emulsifier, an adhesive, cypermethrin and emamectin benzoate into water, stir and mix evenly to obtain component B; wherein the volume ratio of the emulsifier, the adhesive, cypermethrin, emamectin benzoate and water is 0.4:0.6:8.5:5:85.5; wherein the emulsifier is emulsifier OP-10; the adhesive is polyethylene glycol; and the cypermethrin is the cypermethrin microcapsule in Preparation Example 1.

[0029] Example 4 The difference between Example 4 and Example 2 is that the cypermethrin is the cypermethrin microcapsule in Preparation Example 2; and the remaining steps are consistent with Example 2.

[0030] Example 5 The difference between Example 5 and Example 2 is that the cypermethrin is the cypermethrin microcapsule in Preparation Example 3; and the remaining steps are consistent with Example 2.

[0031] Application Examples Application Example 1 Application Example 1 provides a method for using a composite agent for controlling pine trunk borers, comprising the following steps: Use of component A: In November and December, a hole is drilled at a downward angle of 45° at a depth of 5 cm from the base of a Masson pine tree using an electric drill with a diameter of about 1 cm. The number of holes is determined according to the tree's diameter at breast height. When the tree's diameter at breast height is less than 20 cm, the number of holes is 2. When the tree's diameter at breast height is greater than 20 cm, the number of holes is increased by 1 for every 10 cm increase, and the number of holes is increased by 1 for a diameter less than 10 cm. The deep holes are symmetrically distributed to obtain a 6.5 cm deep hole, and then 20 ml of component A in Example 1 is injected; Use of component B: In mid-May and mid-to-late June of the following year, use a drone to spray component B in Example 1 once in the forest area where component A has been injected, at a rate of 5 L / mu.

[0032] Application Example 2 Application Example 2 provides a method for using a composite agent for controlling pine trunk borers, comprising the following steps: Use of component A: In November and December, a hole is drilled at a downward angle of 45° at a depth of 5 cm from the base of a Masson pine tree with an electric drill of about 1 cm, and the number of holes is determined according to the tree's diameter at breast height. When the tree's diameter at breast height is less than 20 cm, the number of holes is 2. When the tree's diameter at breast height is greater than 20 cm, the number of holes is increased by 1 for every increase of 10 cm, and the number of holes is increased by 1 for a diameter less than 10 cm. The deep holes are symmetrically distributed to obtain a 6.5 cm deep hole, and then 20 ml of component A in Example 2 is injected; Use of component B: In mid-May and mid-to-late June of the following year, use a drone to spray component B in Example 2 once in the forest area where component A has been injected, at a rate of 5 L / mu.

[0033] Application Example 3 Application Example 3 provides a method for using a composite agent for controlling pine trunk borers, comprising the following steps: Use of component A: In November and December, a hole is drilled at a downward angle of 45° at a depth of 5 cm from the base of a Masson pine tree with an electric drill of about 1 cm, the number of holes being determined according to the tree's diameter at breast height. When the tree's diameter at breast height is less than 20 cm, the number of holes is 2. When the tree's diameter at breast height is greater than 20 cm, the number of holes is increased by 1 for every increase of 10 cm, and the number of holes is increased by 1 for a diameter less than 10 cm. The deep holes are symmetrically distributed to obtain a 6.5 cm deep hole, and then 20 ml of component A in Example 3 is injected; Use of component B: In mid-May and mid-to-late June of the following year, use a drone to spray component B in Example 3 once in the forest area where component A has been injected, at a rate of 5 L / mu.

[0034] Application Example 4 Application Example 4 provides a method for using a composite agent for controlling pine trunk borers, comprising the following steps: Use of component A: In November and December, a hole is drilled at a downward angle of 45° at a depth of 5 cm from the base of a Masson pine tree with an electric drill of about 1 cm, the number of holes being determined according to the tree's diameter at breast height. When the tree's diameter at breast height is less than 20 cm, the number of holes is 2. When the tree's diameter at breast height is greater than 20 cm, the number of holes is increased by 1 for every 10 cm increase, and 1 is also increased for less than 10 cm, and the deep holes are symmetrically distributed to obtain a 6.5 cm deep hole, and then 20 ml of component A in Example 4 is injected; Use of component B: In mid-May and mid-to-late June of the following year, use a drone to spray component B in Example 4 once in the forest area where component A has been injected, at a rate of 5 L / mu.

[0035] Application Example 5 Application Example 5 provides a method for using a composite agent for controlling pine trunk borers, comprising the following steps: Use of component A: In November and December, a hole is drilled at a downward angle of 45° at a depth of 5 cm from the base of a Masson pine tree with an electric drill of about 1 cm, the number of holes being determined according to the tree's diameter at breast height. When the tree's diameter at breast height is less than 20 cm, the number of holes is 2. When the tree's diameter at breast height is greater than 20 cm, the number of holes is increased by 1 for every 10 cm increase, and the number of holes is increased by 1 for a diameter less than 10 cm. The deep holes are symmetrically distributed to obtain a 6.5 cm deep hole, and then 20 ml of component A in Example 5 is injected; Use of component B: In mid-May and mid-to-late June of the following year, use a drone to spray component B in Example 5 once in the forest area where component A has been injected, at a rate of 5 L / mu.

[0036] Comparative Application Examples Comparative application example 1 Comparative Application Example 1 provides a method for using emamectin benzoate as an insecticide for controlling pests and diseases, comprising the following steps: In November and December, holes were drilled at a downward angle of 45° at a depth of 5 cm from the base of the Masson pine tree using an electric drill with a depth of about 1 cm. The number of holes was determined according to the tree's diameter at breast height. When the tree's diameter at breast height was less than 20 cm, the number of holes was 2. When the tree's diameter at breast height was greater than 20 cm, the number of holes increased by 1 for every increase of 10 cm, and also increased by 1 for a diameter less than 10 cm. The deep holes were symmetrically distributed to obtain a 6.5 cm deep hole, and then 20 ml of component A in Example 1 was injected.

[0037] Comparative Application Example 2 Comparative Application Example 2 provides a method for using emamectin benzoate as an insecticide for preventing and controlling insect diseases, comprising the following steps: In mid-May and mid-to-late June, component B in Example 1 was sprayed once in the Masson pine forest area using a drone in the evening or early morning on sunny days, with an application rate of 5 L / mu.

[0038] Performance test insect control effect: The forest areas of each application example and comparative application example were tested. After application of the pesticide, the percentage decrease in the base number of pine knot weevils and the percentage decrease in the density of pine brown alternating beetles in the forest area were found. The greater the percentage decrease, the better the insect control effect of the pesticide.

[0039] Table 1 Performance test results of the reagents Combining Application Example 1 and Comparative Application Examples 1-2, the insecticide in Example 1 has the best insecticidal effect. It can be seen that the use of component A composed of avermectin benzoate and an emulsifier, and component B composed of an emulsifier, an adhesive, cypermethrin, and avermectin benzoate improves the control effect on pine gall weevils and pine brown alternator.

[0040] Combined with Application Examples 1-3, the insecticide effect of the agent in Example 2 is the best. It can be seen that when preparing component A and component B, the ratio of the raw materials in Example 2 is optimal, so that the agent has the best insecticide effect.

[0041] Combining Application Example 2, Application Example 4 and Application Example 5, the insecticide effect of the agent in Example 4 is the best. It can be seen that when preparing cypermethrin microcapsules, the ratio of the raw materials in the mixed solution A and the mixed solution B in Preparation Example 2, as well as the mixed solution A, the mixed solution B, and the polysorbate-20 solution is optimal, so that the insecticide effect of the cypermethrin microcapsules is better.

[0042] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A composite agent for preventing and controlling pine trunk borers, characterized in that: The invention comprises component A and component B; the raw materials of component A comprise emamectin benzoate and an emulsifier; the raw materials of component B comprise an emulsifier, an adhesive, cypermethrin and emamectin benzoate.

2. A composite agent for controlling pine trunk borers according to claim 1, characterized in that: The volume ratio of avermectin benzoate to emulsifier in component A is (8-10):0.

2.

3. A composite agent for controlling pine trunk borers according to claim 2, characterized in that: The emulsifier includes emulsifier OP-10.

4. A composite agent for controlling pine trunk borers according to claim 1, characterized in that: The volume ratio of the emulsifier, the adhesive, cypermethrin and emamectin benzoate in the B component is 0.4: (0.4-0.6): (7.5-8.5): (2-5).

5. A composite agent for controlling pine trunk borers according to claim 4, characterized in that: Described cypermethrin is pre-treated by following steps: Dissolve cypermethrin in dichloromethane, then add a sustained-release agent and a stabilizer, stir and dissolve, and obtain a mixed solution A; dissolving poly(lactic acid-co-glycolic acid) copolymer in dichloromethane to form a mixed solution B; The mixed solution A and the mixed solution B are mixed, and a polysorbate-20 solution is added to obtain a mixed solution C. The mixed solution C is stirred and sheared to obtain an emulsion, and dichloromethane is removed by rotary evaporation at 40° C. to obtain a suspension, which is filtered, and the filter residue is washed and dried to obtain cypermethrin microcapsules.

6. A composite agent for controlling pine trunk borers according to claim 5, characterized in that: The weight ratio of cypermethrin, dichloromethane, sustained-release agent and stabilizer in the mixed solution A is (0.3-0.32):9:(0.45-0.47):(0.4-0.6); the weight ratio of polylactic acid-glycolic acid copolymer and dichloromethane in the mixed solution B is (0.92-0.94):10; the weight ratio of mixed solution A, mixed solution B and polysorbate-20 solution in the mixed solution C is 1:(1.06-1.07):(1.92-1.94).

7. A composite agent for controlling pine trunk borers according to claim 4, characterized in that: The adhesive includes polyethylene glycol.

8. A method for using the composite agent for controlling pine trunk borers according to any one of claims 1 to 7, characterized in that: The following steps are involved: Use of component A: Add emamectin benzoate and emulsifier into water, stir and mix evenly to obtain component A; in November and December, drill a hole at a depth of 5 cm to 8 cm at a downward angle of 45 degrees with an electric drill of about 1 cm at the base of the tree trunk 5 cm below the ground, and then inject component A; Use of component B: add emulsifier, adhesive, cypermethrin and avermectin benzoate into water, stir and mix evenly to obtain component B; in mid-May and mid-to-late June of the following year, use a drone to spray component B once in the forest area where component A has been injected on sunny evenings or early mornings.

9. The method for using the composite agent for controlling pine trunk borers according to claim 8, characterized in that: When using component A, the number of holes is determined according to the tree's diameter at breast height. When the tree's diameter at breast height is less than 20cm, the number of holes is 2. When the tree's diameter at breast height is greater than 20cm, the number of holes increases by 1 for every increase of 10cm, and also increases by 1 for less than 10cm. The deep holes are symmetrically distributed, and the injection volume is 20ml. When using component B, the application volume is 5L / mu.

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