Composition for improving yolk chromaticity and application thereof

Abstract

The invention provides a composition for improving yolk chromaticity and application thereof, the composition for improving yolk chromaticity comprises ethyl xanthophyll and dehydrated xanthophyll, and the mass ratio of ethyl xanthophyll to dehydrated xanthophyll is 1: (1-5). According to the method, ethyl xanthophyll and dehydrated xanthophyll are compounded according to a specific proportion, so that the chromaticity of yolk can be obviously improved, which cannot be achieved by using xanthophyll, capsanthin and astaxanthin independently or in a combined manner in the prior art. Furthermore, the ethyl xanthophyll, the dehydrated xanthophyll and the xanthophyll are compounded according to a specific proportion, so that the coloring effect is better.

Description

Technical Field

[0001] The invention relates to the field of colorants, in particular to a composition for improving egg yolk hue and application thereof. Background Art

[0002] With the development of social economy and the improvement of living standards, people not only have a greater demand for livestock and poultry products, but also pay more and more attention to the sensory, flavor and nutritional value of the products. For example, for eggs, consumers generally judge a good egg by its shelf life, yolk color, egg size, and whether it has a fishy smell.

[0003] Among them, egg yolk is the main carrier of egg flavor substances, an important factor affecting egg quality, and an indicator for consumers to subjectively evaluate the quality of eggs. Consumers pay more and more attention to the color of egg yolks, and believe that the brighter the color of the egg yolk, the better the egg quality. The color of egg yolks is mainly related to carotenoids. Since laying hens cannot synthesize carotenoids by themselves, they must be ingested from the diet. Corn is often used as an energy feed in the diet of traditional caged laying hens, so lutein in corn plays an important role in the deposition of egg yolk color. In order to seek more reasonable formula design (such as reducing costs, etc.), laying hen feed and breeding companies sometimes use raw materials such as rice and wheat to replace corn, but such raw materials contain almost no lutein. If used in large quantities in laying hen diets, the color of the egg yolk will become lighter or even white. In this case, the color of the egg yolk can be deepened by adding exogenous pigments to the laying hen diet.

[0004] In the prior art, lutein, capsanthin and other pigments are generally added to improve the coloring effect of egg yolks. However, these pigments can only increase the yolk color to about 12 at most and cannot be further improved, which cannot meet the needs of some consumers. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides a composition for improving the yolk color and application thereof. The composition of the present invention can further improve the yolk color to cater to consumer preferences.

[0006] In a first aspect, the present invention provides a composition for improving the yolk color of egg white, comprising ethyl lutein and dehydrated lutein, wherein the mass ratio of the ethyl lutein to the dehydrated lutein is 1:1-5.

[0007] Lutein, also known as plant lutein, is a carotenoid with a chemical formula of C 40 H 56O2, widely found in vegetables, fruits, flowers and other plants. Lutein has been widely used as a colorant in condiments, tobacco, cakes, candies and a variety of feed processing. Capsanthin is also a kind of carotenoids. It is a natural colorant extracted from peppers and has a good coloring effect. However, using them alone or in combination for egg yolk coloring has limited effect on improving the yolk color and cannot satisfy consumers well.

[0008] The present invention unexpectedly discovered that combining ethyl lutein and dehydrated lutein in a specific ratio can significantly improve the chromaticity of egg yolk, and the effect is better than the prior art.

[0009] Among them, ethyl lutein is 3'-ethyl lutein, English name 3'-ethyl lutein ether, chemical formula is C 42 H 60 O2, can be prepared by chemical reaction. Dehydrated lutein is dehydrated lutein I, the chemical formula is C 40 H 54 O. Dehydrated lutein I and lutein are both available in finished products on the market.

[0010] In some embodiments of the present invention, the mass ratio of the ethyl lutein to the dehydrated lutein is 1:1-4, preferably 1:1-3, and more preferably 1:1-2.

[0011] In some embodiments of the present invention, the composition for improving egg yolk color consists of ethyl lutein and dehydrated lutein.

[0012] In some embodiments of the present invention, the ethyl lutein and the dehydrated lutein exist in monomers, esters or other stable forms.

[0013] In some embodiments of the present invention, the composition further comprises lutein, and the lutein exists in a monomer, ester or other stable form.

[0014] On the basis of the composition of ethyl lutein and dehydrated lutein, the yolk coloring effect is better when lutein is combined with ethyl lutein and dehydrated lutein. However, the coloring effect is not ideal when lutein is combined with only one of ethyl lutein and dehydrated lutein. Among them, lutein has poor stability and is more sensitive to light, heat, etc. Therefore, it can be added in the form of a monomer, or in the form of lutein ester or other stable forms.

[0015] In some embodiments of the present invention, the mass ratio of the ethyl lutein to the lutein is 1:1-20, preferably 1:1-15, more preferably 1:1-10, and more preferably 1:1-5.

[0016] In some embodiments of the present invention, the composition for improving the yolk color is composed of ethyl lutein, dehydrated lutein and lutein. Preferably, the mass ratio of the three is 1:1-2:1-5.

[0017] In the present invention, there is no special requirement for the mass ratio of trans-lutein in the lutein.

[0018] In a second aspect, the present invention provides a nutritional composition comprising any one of the above-mentioned compositions for improving egg yolk color.

[0019] In some embodiments of the present invention, the nutritional composition further comprises one or more of oil, vitamins, proteins, carbohydrates, carriers and adjuvants.

[0020] In the present invention, the term "nutritional composition" means a composition that provides nutrition to a subject.

[0021] Among them, the carrier and the auxiliary agent can be selected according to the specific needs of the nutritional composition. For example, when the nutritional composition is made into tablets, magnesium stearate is usually added as a glidant. For another example, when the nutritional composition is made into a suspension, water is generally added as a carrier, and auxiliary agents such as surfactants and antioxidants may be appropriately added.

[0022] In a third aspect, the present invention provides a feed additive comprising any one of the above-mentioned compositions for improving egg yolk color.

[0023] The preparation form of the feed additive is not limited and can be common preparation forms such as powder, liquid or microcapsule.

[0024] In a fourth aspect, the present invention also provides an application of the feed additive in improving egg yolk color, comprising: adding the feed additive to the daily feed of laying fowls for feeding, wherein the mass proportion of the composition for improving egg yolk color in the feed is 0.01-1%, preferably 0.2%.

[0025] In some embodiments of the present invention, the composition for improving egg yolk color accounts for 0.2% by weight in the feed.

[0026] The composition provided by the present invention has the effect of improving the yolk color of egg yolks, and thus can be used as a feed additive and added to the daily feed of laying fowls for feeding, so that the color of the eggs produced by them is greatly improved to meet the preferences of consumers.

[0027] The egg-laying poultry of the present invention includes chickens, ducks, geese, etc., and is particularly suitable for chickens, wherein the type of chicken is not limited. Although only Hailan gray egg-laying chickens are listed in the embodiments of the present invention, the common breeds of breeding chickens such as Hailan series, Jingfen series, Dayou Jinfeng, Nongda series, and Roman series have the same good effect, and the inventors have done experimental verification.

[0028] The present invention provides a composition for improving the chromaticity of egg yolk and an application thereof. By compounding ethyl lutein and dehydrated lutein in a specific ratio, the chromaticity of egg yolk can be significantly improved, which is impossible to achieve by using lutein, capsanthin, and astaxanthin alone or in combination in the prior art.

[0029] Furthermore, ethyl lutein, dehydrated lutein and lutein are compounded in a specific ratio to achieve a better coloring effect.

[0030] It should be noted that the endpoints and any values ​​of the ranges disclosed in this article are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, and these numerical ranges should be regarded as specifically disclosed in this article.

[0031] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "specific implementations", or "some specific implementations" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the embodiment of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] If no specific techniques or conditions are specified in the examples, the techniques or conditions described in the literature in the field or the product instructions are used. If no manufacturer is specified for the reagents or instruments used, they are all conventional products that can be purchased through regular channels.

[0034] The ethyl lutein, anhydrous lutein and lutein in the colorant composition of the present invention can be detected by high performance liquid chromatography, and the detection conditions are as follows:

[0035] Mobile phase: n-hexane: acetone = 81:19, isocratic elution is used for analysis (elution conditions are shown in Table 1); flow rate is controlled during elution: 1.1 mL / min; column temperature: 28°C; chromatographic column: Kromasil 100-5SIL (150×4.6 mm, particle size 5 μm); UV detector wavelength: 473 nm; injection volume: 20 μL. The retention time of dehydrated lutein is about 3.6-3.9 min, and the retention time of ethyl lutein is about 3.9-4.3 min. The content of each component in the composition is calculated by area normalization method, and the percentage of the peak area of ​​each component to the total peak area is the content ratio of each component in the composition.

[0036] The preparation process of 3'-ethyl lutein of the present invention is as follows:

[0037] 1. Solution preparation: Add 100 μL of concentrated hydrochloric acid to 10 mL of anhydrous ethanol, mix thoroughly to obtain acidic ethanol, and place in a 4°C refrigerator for later use.

[0038] 2. Sample preparation. Weigh 0.1 mg of lutein crystals and slowly add acidic ethanol (about 5 mL) to it until the lutein crystals are completely dissolved. Place the mixture in a refrigerator overnight to react for 24 hours to ensure that the reaction is complete. Afterwards, add 10 mL of deionized water to the mixture to quench the reaction, and then add 10 mL of saturated sodium bicarbonate solution to neutralize the reaction product to obtain a mixture containing ethyl lutein.

[0039] 3. Separation and purification. Add 100 mL of dichloromethane to the mixed solution (3) for liquid-liquid extraction. After sufficient shaking, let stand to separate the layers and collect the dichloromethane layer. Repeat the extraction three times and combine the three dichloromethane layers. Concentrate and dry under vacuum in a cold water bath to obtain a powder that is 3'-ethyl lutein.

[0040] Example 1

[0041] This embodiment provides a composition for improving the yolk color of egg white, which is composed of ethyl lutein and dehydrated lutein in a mass ratio of 1:5.

[0042] The composition provided in this embodiment was added as a feed additive to a feed (ordinary diet without additional coloring agent) at an addition amount of 0.2wt%. A total of 60 35-week-old Hy-line gray laying hens were selected, and 6 replicates were treated in the experiment, with 10 chickens in each replicate, and the number of test days was 32 days, including a 4-day adaptation period and a 28-day test period. On the 0th day, 14th day, and 28th day of the test period, 5 eggs were randomly collected from each replicate and the color of the egg yolk was measured using the Roche colorimetric fan (the result was the average value of the yolk color of these 30 eggs).

[0043] Example 2

[0044] This embodiment provides a composition for improving the yolk color of egg white, which is composed of ethyl lutein and dehydrated lutein in a mass ratio of 1:3.5.

[0045] The composition of this example was used as a feed additive, and the egg yolk color was measured according to the same test settings as in Example 1.

[0046] Example 3

[0047] This embodiment provides a composition for improving the yolk color of egg white, which is composed of ethyl lutein and dehydrated lutein in a mass ratio of 1:2.5.

[0048] The composition of this example was used as a feed additive, and the egg yolk color was measured according to the same test settings as in Example 1.

[0049] Example 4

[0050] This embodiment provides a composition for improving the yolk color of egg white, which is composed of ethyl lutein and dehydrated lutein in a mass ratio of 1:1.

[0051] The composition of this example was used as a feed additive, and the egg yolk color was measured according to the same test settings as in Example 1.

[0052] Example 5

[0053] This embodiment provides a composition for improving the yolk color of egg white, which is composed of ethyl lutein, dehydrated lutein and lutein in a mass ratio of 1:2:20.

[0054] The composition of this example was used as a feed additive, and the egg yolk color was measured according to the same test settings as in Example 1.

[0055] Example 6

[0056] This embodiment provides a composition for improving the yolk color of egg white, which is composed of ethyl lutein, dehydrated lutein and lutein in a mass ratio of 1:2:13.

[0057] The composition of this example was used as a feed additive, and the egg yolk color was measured according to the same test settings as in Example 1.

[0058] Example 7

[0059] This embodiment provides a composition for improving the yolk color of egg white, which is composed of ethyl lutein, dehydrated lutein and lutein in a mass ratio of 1:2:8.

[0060] The composition of this example was used as a feed additive, and the egg yolk color was measured according to the same test settings as in Example 1.

[0061] Example 8

[0062] This embodiment provides a composition for improving the yolk color of egg white, which is composed of ethyl lutein, dehydrated lutein and lutein in a mass ratio of 1:2:4.

[0063] The composition of this example was used as a feed additive, and the egg yolk color was measured according to the same test settings as in Example 1.

[0064] Example 9

[0065] This embodiment provides a composition for improving the yolk color of egg white, which is composed of ethyl lutein, dehydrated lutein and lutein in a mass ratio of 1:4.5:5.

[0066] The composition of this example was used as a feed additive, and the egg yolk color was measured according to the same test settings as in Example 1.

[0067] Example 10

[0068] This embodiment provides a composition for improving the yolk color of egg white, which is composed of ethyl lutein, dehydrated lutein and lutein in a mass ratio of 1:4:5.

[0069] The composition of this example was used as a feed additive, and the egg yolk color was measured according to the same test settings as in Example 1.

[0070] Embodiment 11

[0071] This embodiment provides a composition for improving the yolk color of egg white, which is composed of ethyl lutein, dehydrated lutein and lutein in a mass ratio of 1:3:5.

[0072] The composition of this example was used as a feed additive, and the egg yolk color was measured according to the same test settings as in Example 1.

[0073] Blank Group

[0074] A total of 60 Hy-Line gray laying hens aged 35 weeks were selected. No colorant was added to the feed of the blank test group. Six replicates were treated in the blank test, with 10 chickens in each replicate. The test days were 32 days, including a 4-day adaptation period and a 28-day test period. Five eggs were randomly collected from each replicate on days 0, 14, and 28 of the test period, and the color of the egg yolk was determined using the Roche colorimetric fan.

[0075] Comparative Example 1

[0076] A total of 60 35-week-old Hy-Line gray laying hens were selected, and the experiment was set up according to the colorant composition ratio (mass ratio, the same below) of lutein: capsanthin: astaxanthin = 1:0:0. The composition was added in the feed at a rate of 0.2%. Six replicates were treated in the experiment, with 10 chickens in each replicate. The experimental days were 32 days, including an adaptation period of 4 days and a test period of 28 days. On days 0, 14, and 28 of the test period, 5 eggs were randomly collected from each replicate, and the color of the egg yolk was determined using the Roche colorimetric fan.

[0077] Comparative Example 2

[0078] The colorant composition ratio is lutein: capsanthin: astaxanthin = 0:1:0, and the test setting is performed, and other conditions are the same as those of Comparative Example 1.

[0079] Comparative Example 3

[0080] The colorant composition ratio is lutein: capsanthin: astaxanthin = 0:0:1, and the test setting is performed. Other conditions are the same as those in Comparative Example 1.

[0081] Comparative Example 4

[0082] The ratio of the colorant composition is lutein: capsanthin: astaxanthin = 1:1:0, and the test is set up, and other conditions are the same as those of Comparative Example 1.

[0083] Comparative Example 5

[0084] The ratio of the colorant composition is lutein: capsanthin: astaxanthin = 0:1:1, and the test is set up, and other conditions are the same as those of Comparative Example 1.

[0085] Comparative Example 6

[0086] The colorant composition ratio is lutein: capsanthin: astaxanthin = 1:0:1, and the test setting is performed, and other conditions are the same as those of Comparative Example 1.

[0087] Comparative Example 7

[0088] The ratio of the colorant composition is lutein: capsanthin: astaxanthin = 1:1:1, and the amount of the colorant composition added to the feed is 0.2%. The test settings are carried out, and other conditions are the same as those of Comparative Example 1.

[0089] Comparative Example 8

[0090] The ratio of the colorant composition is lutein: capsanthin: astaxanthin = 1:1:1, and the amount of the colorant composition added to the feed is 0.4%. The test settings are carried out, and other conditions are the same as those of Comparative Example 1.

[0091] Comparative Example 9

[0092] The colorant composition is ethyl lutein and dehydrated lutein in a mass ratio of 5:1. The test is set up, and other conditions are the same as those in Comparative Example 1.

[0093] Comparative Example 10

[0094] The colorant composition is ethyl lutein and lutein in a mass ratio of 1:1. The test is set up, and other conditions are the same as those in Comparative Example 1.

[0095] Comparative Example 11

[0096] The colorant composition is dehydrated lutein and lutein in a mass ratio of 1:1. The test is set up, and other conditions are the same as those in Comparative Example 1.

[0097] Results The egg yolk coloring of the embodiment, blank group and comparative example is shown in Table 1.

[0098] Table 1

[0099] Group Egg yolk color Example 1 12.0 Example 2 12.5 Example 3 13.0 Example 4 13.5 Example 5 13.5 Example 6 13.8 Example 7 14.5 Example 8 15.0 Example 9 13.0 Example 10 13.5 Embodiment 11 13.8 Blank Group 6.0 Comparative Example 1 10.0 Comparative Example 2 9.0 Comparative Example 3 9.5 Comparative Example 4 11.0 Comparative Example 5 10.5 Comparative Example 6 11.0 Comparative Example 7 11.5 Comparative Example 8 12.0 Comparative Example 9 11 Comparative Example 10 10.5 Comparative Example 11 10

[0100] It can be seen from Table 1 that the composition of the present invention has an excellent coloring effect on egg yolk compared with other colorants.

[0101] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A composition for improving egg yolk color, characterized in that It comprises ethyl lutein and dehydrated lutein, and the mass ratio of the ethyl lutein to the dehydrated lutein is 1:1-5.

2. The composition for improving egg yolk color according to claim 1, characterized in that The mass ratio of the ethyl lutein to the dehydrated lutein is 1:1-4, preferably 1:1-3, and more preferably 1:1-2.

3. The composition for improving egg yolk color according to claim 1 or 2, characterized in that: The ethyl lutein and the dehydrated lutein exist in the form of monomers, esters or other stable forms.

4. The composition for improving egg yolk color according to any one of claims 1 to 3, characterized in that: The composition also includes lutein, which is present in monomer, ester or other stable forms.

5. The composition for improving egg yolk color according to claim 4, characterized in that: The mass ratio of the ethyl lutein to the lutein is 1:1-20, preferably 1:1-15, more preferably 1:1-10, and even more preferably 1:1-5.

6. A nutritional composition, characterized in that The invention comprises a composition for improving egg yolk color as described in any one of claims 1 to 5.

7. The nutritional composition according to claim 6, characterized in that The nutritional composition further comprises one or more of oil, vitamins, proteins, carbohydrates, carriers and adjuvants.

8. A feed additive, characterized in that: The invention comprises a composition for improving egg yolk color as described in any one of claims 1 to 5.

9. The feed additive according to claim 8, characterized in that The feed additive is in the form of powder, liquid or microcapsule.

10. Use of the feed additive according to claim 8 or 9 in improving egg yolk color, characterized in that: The feed additive is added to the daily feed of laying fowls for feeding, and the composition for improving egg yolk color accounts for 0.01-1% by weight in the feed, preferably 0.2%.