Composition for coloring animal tissues, feed additive, feed and application thereof

Abstract

The invention relates to the technical field of feed additives, in particular to a composition for coloring animal tissues, a feed additive, feed and application thereof. The composition for coloring the animal tissues, provided by the invention, comprises ethyl lutein and dehydrated lutein in a mass ratio of (1-12): 1. The composition provided by the invention can significantly improve the chromaticity of animal tissues, and has a good coloring effect on the animal tissues. Compared with common colorants such as lutein and zeaxanthin, the composition can generate a better coloring effect when being used for feeding animals, especially has a very excellent coloring effect on subwing skin, and can be used for improving the color quality of animal products.

Description

Technical Field

[0001] The present invention relates to the technical field of feed additives, in particular to a composition for animal tissue coloring, a feed additive, a feed and applications thereof. Background Art

[0002] With socioeconomic development and rising living standards, demand for livestock, poultry, and aquatic products is increasing, and consumers are placing increasing emphasis on their sensory qualities, flavor, and nutritional value. When purchasing livestock, poultry, and aquatic products, color is their primary consideration. Therefore, the color of livestock carcasses, the color of aquatic products, and the color of egg yolks are crucial factors influencing sales and prices. Adding colorants to livestock, poultry, and aquatic feed can improve their color, aroma, flavor, and nutritional qualities. Take, for example, the highly sought-after "Three Yellow" (yellow feathers, yellow skin, and yellow beak) broilers. The coloration of these "Three Yellow" broilers is a key sensory indicator of product quality, directly influencing market demand and prices.

[0003] In recent years, due to the widespread increase in intensive farming practices, the skin color of yellow-feathered broiler chickens often fails to meet commercial requirements at market entry. To improve the color of yellow-feathered broiler chickens' beaks, tarsus, underwing skin, and abdominal fat, existing technologies primarily use colorants such as lutein and zeaxanthin, as well as canthaxanthin, to improve the coloring. However, these colorants still fail to achieve the desired coloring effect. Therefore, the development of colorants that can effectively enhance the coloring of poultry and other animal tissues is of great significance. Summary of the Invention

[0004] The invention provides a composition for animal tissue coloring, a feed additive, a feed and applications thereof.

[0005] During the process of developing a new colorant for animal tissue coloring, the present invention unexpectedly discovered that dehydrolutein, dehydrated lutein, and ethyl lutein can be used alone or as a combination thereof to color animal tissues. Furthermore, it was unexpectedly discovered that ethyl lutein has a good coloring effect on the skin under the wings of three-yellow chickens. In particular, when used in combination, ethyl lutein and dehydrated lutein are compounded in a specific ratio, or ethyl lutein, dehydrated lutein, and dehydrolutein are compounded in a specific ratio, which produces a synergistic effect on the coloring effect of the skin under the wings of three-yellow chickens. The coloring effect is significantly better than the coloring effect of the above components used alone at the same dosage, and is also significantly better than commonly used colorants such as lutein and zeaxanthin.

[0006] Specifically, the present invention provides the following technical solutions:

[0007] In a first aspect, the present invention provides a composition for animal tissue coloring, wherein the composition comprises ethyl lutein and dehydrated lutein in a mass ratio of (1-12):1.

[0008] In the present invention, the ethyl lutein is 3'-ethyl lutein, whose English name is 3'-ethyl lutein ether and whose chemical formula is C 42 H 60 O2, the dehydrated xanthophyll is dehydrated xanthophyll I, the English name is: Anhydrolutein I, the chemical formula is: C 40 H 54 O.

[0009] Preferably, the composition comprises ethyl lutein and anhydro lutein in a mass ratio of (1-10):1.

[0010] Animal experiments have shown that when ethyl lutein and dehydrated lutein are mixed in the above-mentioned mass ratio and fed to animals, the animal tissues can produce a better coloring effect, and can also produce a synergistic effect in the coloring of tissues such as the underwing skin. That is, at the same feeding amount, the coloring effect of ethyl lutein and dehydrated lutein in the above-mentioned ratio is significantly better than the coloring effect of either one fed separately, especially in the coloring of the underwing skin.

[0011] In some embodiments of the present invention, the composition consists of ethyl lutein and dehydrated lutein in a mass ratio of (1-10):1.

[0012] In some other embodiments of the present invention, the composition further comprises dehydrolutein; in the composition, the mass ratio of ethyl lutein to dehydrolutein is (1-5):1.

[0013] Preferably, the dehydrogenated xanthophyll is 3'-dehydrogenated xanthophyll, the English name is: 3'-oxolutein, the chemical formula is C 40 H 54 O2.

[0014] In the present invention, both dehydrated lutein I and 3'-dehydrolutein can be purchased from commercial sources. 3'-Ethyl lutein has been disclosed in the literature "Chi J. Oxidation of Zeaxanthin and characterization of 3'-Alkyl Lutein ethers [J]. 2001" and can be prepared using known methods or purchased from commercial sources.

[0015] In some embodiments of the present invention, the composition comprises ethyl lutein, dehydrated lutein and dehydrogenated lutein, wherein the mass ratio of ethyl lutein to dehydrated lutein is (1-10):1, and the mass ratio of ethyl lutein to dehydrogenated lutein is (1-5):1.

[0016] Preferably, the composition comprises ethyl lutein, dehydrated lutein and dehydrogenated lutein, wherein the mass ratio of ethyl lutein to dehydrated lutein is (1-3):1, and the mass ratio of ethyl lutein to dehydrogenated lutein is (1-5):1.

[0017] Animal experiments have shown that when ethyl lutein, dehydrated lutein and dehydrogenated lutein are mixed in the above-mentioned mass ratio and fed to animals, the animal tissues can produce better coloring effects, and can also produce a synergistic effect in the coloring of tissues such as the underwing skin. That is, at the same feeding amount, the coloring effect of ethyl lutein, dehydrated lutein and dehydrogenated lutein in the above-mentioned ratio is significantly better than the coloring effect of feeding the three alone, especially in the coloring of the underwing skin.

[0018] Compared with commonly used colorants (such as lutein, zeaxanthin, etc.), the colorant composition described above can achieve better coloring effect at a significantly lower feeding amount, which is of great significance in reducing the amount and cost of colorant addition and improving coloring effect.

[0019] The colorant composition may consist solely of the above components, or may be further combined with other colorants.

[0020] In a second aspect, the present invention provides any of the following uses of the above-mentioned composition:

[0021] (1) Application in animal tissue coloring;

[0022] (2) Application in the preparation of products with animal tissue coloring function;

[0023] (3) Application in the preparation of feed or feed additives;

[0024] (4) Application in improving the colorimetry of animal tissues.

[0025] In the above application, the animal is poultry (such as chicken, duck, goose, quail, pigeon, etc.) or aquatic animal (such as fish, shrimp, crab or shellfish, etc.), preferably poultry, more preferably chicken, duck, goose, quail or pigeon, most preferably chicken.

[0026] In the above application, the tissue is preferably skin or a skin derivative, more preferably beak, tarsus or underwing skin, and most preferably underwing skin. The colorant composition of the present invention is particularly suitable for improving the coloring effect of poultry underwing skin.

[0027] In the applications of (1) and (4) above, the composition is fed to animals to color the animal tissues or to increase the color of the animal tissues.

[0028] In the applications of (2) and (3) above, the composition is used as a colorant for the product, feed or feed additive.

[0029] In a third aspect, the present invention provides a feed or a feed additive, wherein the feed or the feed additive comprises the composition described above.

[0030] Preferably, the feed can be compound feed, oil feed, vitamin feed, protein feed, energy (carbohydrate) feed, etc.

[0031] In addition to the above composition, the feed may also contain one or more selected from energy substances, proteins, oils, vitamins, and minerals.

[0032] In a fourth aspect, the present invention provides the use of a combination of one or more selected from dehydrolutein, dehydrated lutein, and ethyl lutein in coloring animal tissue or improving the color of animal tissue.

[0033] Preferably, the dehydrated lutein is dehydrated lutein I, the dehydrolutein is 3'-dehydrolutein, and the ethyl lutein is 3'-ethyl lutein.

[0034] Preferably, the use comprises feeding an animal a combination of one or more selected from dehydrolutein, anhydrolutein, and ethyllutein. Feeding an animal a combination of one or more selected from dehydrolutein, anhydrolutein, and ethyllutein can pigment the animal's skin or skin derivative.

[0035] In the above application, the animal is poultry or aquatic animal. Preferably, it is poultry. More preferably, it is chicken, duck, goose, quail, or pigeon. Most preferably, it is chicken.

[0036] In the above applications, the tissues include skin or skin derivatives, muscle, fat, eggs, etc. Among them, the skin derivatives include beaks, feathers, claws, scales, hair, hooves, nails, etc.

[0037] Preferably, the tissue is skin or a skin derivative, more preferably beak, tarsus or underwing skin, most preferably underwing skin.

[0038] Preferably, the combination of multiple types of the above-mentioned dehydrolutein, anhydrolutein and ethyllutein includes: ethyllutein and dehydrolutein, or ethyllutein, anhydrolutein and dehydrolutein.

[0039] Preferably, the application comprises feeding ethyl lutein and dehydrated lutein to animals, wherein the mass ratio of ethyl lutein to dehydrated lutein is (1-10):1;

[0040] Alternatively, the application comprises feeding ethyl lutein, dehydrated lutein and dehydrogenated lutein to animals, wherein the mass ratio of ethyl lutein to dehydrated lutein is (1-10):1, and the mass ratio of ethyl lutein to dehydrogenated lutein is (1-5):1.

[0041] In a fifth aspect, the present invention provides a method for coloring animal tissues, comprising: feeding an animal with the composition, feed, or feed additive described above.

[0042] In a sixth aspect, the present invention provides a method for improving the colorimetry of animal tissues, the method comprising: feeding the animal with the composition, feed or feed additive described above.

[0043] In a seventh aspect, the present invention provides a method for producing poultry with yellow skin or skin derivatives, the method comprising: feeding the animal with the composition, feed or feed additive described above.

[0044] Preferably, in the above method, the poultry with yellow skin or skin derivatives is a three-yellow chicken.

[0045] The beneficial effects of the present invention include at least: the composition for coloring animal tissues provided by the present invention has a good coloring effect on animal tissues, significantly improving the chroma of animal tissues. Compared with commonly used colorants (such as lutein and zeaxanthin), feeding animals with this composition can produce a better coloring effect with a smaller feeding amount, especially for the skin under the wings. Therefore, it has promising application prospects in livestock and poultry farming. DETAILED DESCRIPTION

[0046] To make the objectives, technical solutions, and advantages of the present invention more clear, the technical solutions of the present invention are described clearly and completely below. Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

[0047] The dehydrolutein, anhydrolutein and ethyllutein in the composition for animal tissue coloring of the present invention can be detected by high performance liquid chromatography under the following conditions:

[0048] The analysis was performed using isocratic elution with a mobile phase of n-hexane:acetone (81:19). The flow rate during elution was controlled at 1.1 mL / min. The column temperature was 28°C. The chromatographic column was Kromasil 100-5SIL (150×4.6 mm, particle size 5 μm). The UV detector wavelength was 473 nm. The injection volume was 20 μL. The retention time of dehydrolutein was approximately 3.2-3.6 min, the retention time of anhydrolutein was approximately 3.6-3.9 min, and the retention time of ethyllutein was approximately 3.9-4.3 min. The content of each component in the composition was calculated using the area normalization method. The percentage of each component's peak area to the total peak area represents the proportion of each component in the composition.

[0049] The dehydrolutein used in the following examples is 3'-dehydrolutein, and the dehydrated lutein is dehydrated lutein I, both of which are commercially available. The ethyl lutein used is 3'-ethyl lutein, which can be prepared by the following method:

[0050] 1. Solution preparation: Add 100 μL of concentrated hydrochloric acid to 10 mL of anhydrous ethanol, mix thoroughly to obtain acidic ethanol, and place in a 4°C refrigerator for later use.

[0051] 2. Sample preparation: Weigh 0.1 mg of lutein crystals and slowly add acidic ethanol (about 5 mL) to it until the lutein crystals are completely dissolved; place the mixture in a refrigerator overnight to react for 24 hours. After the reaction is complete, add 10 mL of deionized water to the mixture to quench the reaction, and then add 10 mL of saturated sodium bicarbonate solution to neutralize the reaction product to obtain a mixture containing ethyl lutein.

[0052] 3. Separation and purification: Add 100 mL of dichloromethane to the mixed solution obtained in step 2 for liquid-liquid extraction. After sufficient shaking, let it stand to separate the layers and collect the dichloromethane layer; repeat the extraction three times and combine the three dichloromethane layers; vacuum concentrate and dry in a cold water bath to obtain a powder that is 3'-ethyl lutein.

[0053] Example 1

[0054] This embodiment provides a composition for animal tissue coloring, which includes ethyl lutein and dehydrated lutein in a mass ratio of 3:2.

[0055] Example 2

[0056] This embodiment provides a composition for animal tissue coloring, which comprises ethyl lutein and dehydrated lutein in a mass ratio of 10:1.

[0057] Example 3

[0058] This embodiment provides a composition for animal tissue coloring, which comprises ethyl lutein and dehydrated lutein in a mass ratio of 6:1.

[0059] Example 4

[0060] This embodiment provides a composition for animal tissue coloring, which includes ethyl lutein and dehydrated lutein in a mass ratio of 3:1.

[0061] Example 5

[0062] This embodiment provides a composition for animal tissue coloring, which includes ethyl lutein and dehydrated lutein in a mass ratio of 1:1.

[0063] Example 6

[0064] This embodiment provides a composition for animal tissue coloring, which includes ethyl lutein, dehydrated lutein, and dehydrolutein in a mass ratio of 5:5:1.

[0065] Example 7

[0066] This embodiment provides a composition for animal tissue coloring, which includes ethyl lutein, dehydrated lutein, and dehydrolutein in a mass ratio of 3:2:1.

[0067] Example 8

[0068] This embodiment provides a composition for animal tissue coloring, which includes ethyl lutein, dehydrated lutein, and dehydrolutein in a mass ratio of 1:1:1.

[0069] Example 9

[0070] This embodiment provides a colorant, which is ethyl lutein.

[0071] Example 10

[0072] This embodiment provides a colorant, which is dehydrated lutein.

[0073] Example 11

[0074] This embodiment provides a colorant, which is dehydrolutein.

[0075] Comparative Example 1

[0076] This comparative example provides a colorant, which is lutein.

[0077] Comparative Example 2

[0078] This comparative example provides a colorant, which is zeaxanthin.

[0079] Comparative Example 3

[0080] This comparative example provides a colorant, which is epoxy lutein.

[0081] Comparative Example 4

[0082] This comparative example provides a composition for animal tissue coloring, which comprises lutein and zeaxanthin in a mass ratio of 1:1.

[0083] Experimental example: Animal experiment to verify the coloring effect

[0084] Yellow-feathered broilers were used as experimental animals to test the coloring effects of the compositions or colorants of the Examples and Comparative Examples on animal tissues, as follows:

[0085] Blank group

[0086] Forty-eight 60-day-old female yellow-feathered broiler chickens (Sanhuang chickens, initial weight 1279 ± 81 g) were used. No colorant was added to the blank control diet. Six replicates of eight birds were included in each replicate. The experiment lasted 32 days, including a 4-day acclimatization period and a 28-day test period. After the 28-day test period, three birds were randomly selected from each replicate. The yellowness of the tarsus and beak was measured using a colorimetric fan, and the yellowness of the skin under the wing area on the flank was measured using a colorimeter.

[0087] experimental group

[0088] 60-day-old female yellow-feathered broilers (three yellow chickens, initial body weight 1279 ± 81g) were used to set test groups 1-16, wherein test groups 1-11 were fed the compositions or colorants of Examples 1-11, respectively, and the composition or colorant was added in an amount of 0.2% in the feed; test groups 12-15 were fed the compositions or colorants of Comparative Examples 1-4, respectively, and the composition or colorant was added in an amount of 0.2% in the feed; and test group 16 was fed the composition of Comparative Example 4, and the composition or colorant was added in an amount of 0.4% in the feed. Each test group was repeated 6 times, with 8 chickens in each repeat, and the test days were 32 days, with an adaptation period of 4 days and a test period of 28 days. After the end of the 28-day test period, 3 chickens were randomly selected from each repeat of each group, and the yellowness values ​​of the tarsus and beak of the chicken were measured using a colorimetric fan, and the yellowness value of the skin under the wing of the chicken's flank was measured using a colorimeter.

[0089] The results of each group of chickens were averaged, and the results are shown in Table 1. The results show that the compositions of Examples 1-8 were significantly superior to the commonly used colorants used at the same dosage (Comparative Examples 1, 2, and 4) in coloring the tarsus, beak, and underwing skin of yellow-feathered broilers. The coloring effect on the beak and underwing skin of yellow-feathered broilers was significantly superior to the colorant composition of Comparative Example 4 used at twice the dosage. Compared with dehydrated lutein, dehydrolutein, and ethyl lutein, another lutein derivative, epoxy lutein, had a poorer coloring effect (Comparative Example 3). When ethyl lutein and dehydrated lutein were used in combination in a certain ratio (Examples 1-5), the two produced a synergistic effect in coloring the beak and underwing skin. The coloring effect of the combination was significantly superior to that of either used alone, with the coloring effect on underwing skin being particularly outstanding. When ethyl lutein, dehydrated lutein and dehydrogenated lutein are used in combination (Examples 6-8), the three can produce a synergistic effect in the coloring of the beak, underwing skin and tarsus plantar. The coloring effect of the combination is significantly better than the coloring effect of the three used alone, among which the coloring effect on the underwing skin is particularly outstanding.

[0090] Table 1 Coloration of the beak, tarsus and underwing skin of chickens in the blank group and the experimental group

[0091]

[0092]

[0093] Note: The a value indicates the degree of shift from green to red in the color. The higher the a value, the redder the color.

[0094] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A composition for animal tissue coloring, characterized in that: The composition comprises ethyl lutein and dehydrated lutein in a mass ratio of (1-12):

1.

2. The composition according to claim 1, characterized in that The dehydrated lutein is dehydrated lutein I, and the ethyl lutein is 3'-ethyl lutein; Preferably, the composition comprises ethyl lutein and anhydrolutein in a mass ratio of (1-10):

1.

3. The composition according to claim 1 or 2, characterized in that The composition further comprises dehydrolutein; In the composition, the mass ratio of ethyl lutein to dehydrolutein is (1-5):1; Preferably, the dehydrolutein is 3'-dehydrolutein.

4. Any of the following uses of the composition according to any one of claims 1 to 3: (1) Application in animal tissue staining; (2) Application in the preparation of products with animal tissue coloring function; (3) Application in the preparation of feed or feed additives; (4) Application in improving the colorimetry of animal tissues.

5. The use according to claim 4, characterized in that: The animal is poultry or aquatic animal, preferably chicken, duck, goose, quail or pigeon; And / or, the tissue is skin or a skin derivative, preferably beak, tarsus or underwing skin.

6. The use according to claim 4 or 5, characterized in that: By feeding the composition to an animal, the animal tissue is colored or the color of the animal tissue is improved.

7. A feed or feed additive, characterized in that: The feed or feed additive comprises the composition according to any one of claims 1 to 3.

8. Use of one or more combinations selected from dehydrogenated lutein, dehydrated lutein and ethyl lutein in coloring animal tissues or improving the chromaticity of animal tissues; Preferably, the dehydrated lutein is dehydrated lutein I, the dehydrolutein is 3'-dehydrolutein, and the ethyl lutein is 3'-ethyl lutein.

9. The use according to claim 8, characterized in that: The application comprises feeding the animal with a combination of one or more selected from dehydro-lutein, anhydro-lutein and ethyl-lutein.

10. The use according to claim 8 or 9, characterized in that: The application comprises feeding ethyl lutein and dehydrated lutein to animals, wherein the mass ratio of ethyl lutein to dehydrated lutein is (1-10):1; Alternatively, ethyl lutein, anhydrolutein and dehydrolutein are fed to animals, wherein the mass ratio of ethyl lutein to anhydrolutein is (1-10):1, and the mass ratio of ethyl lutein to dehydrolutein is (1-5):1.

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