Composition containing ceramide and collagen tripeptide and preparation method thereof

Abstract

The invention relates to a composition containing ceramide and collagen tripeptide and a preparation method thereof, and belongs to the technical field of health care products. The invention discloses a composition containing ceramide and collagen tripeptide. The composition is prepared from the following raw materials: collagen tripeptide, rice bran oil powder, a herba houttuyniae extract, pineapple powder, konjaku flour, a grape seed extract, olive fruit powder, carnosine, pomegranate concentrated powder and a whey fermentation product. The grape seed extract, the olive fruit powder, the carnosine and the pomegranate concentrated powder inhibit the Maillard reaction of the collagen peptide and improve the utilization rate of collagen tripeptide; ceramide rich in the rice bran oil powder is a natural skin moisturizing factor, and is matched with the pineapple powder and the konjaku flour which have a keratin metabolism effect to maintain the health of cuticles; the herba houttuyniae extract and the whey fermentation product are matched to have an excellent anti-inflammatory effect, so that the components enhance the skin barrier in multiple dimensions through collagen synthesis, cuticle maintenance, anti-inflammatory and anti-oxidation.

Description

Technical Field

[0001] The invention belongs to the technical field of health care products and relates to a composition containing ceramide and collagen tripeptide and a preparation method thereof. Background Art

[0002] In recent years, with the increasing prominence of skin barrier function damage (such as dryness, sensitivity, aging, etc.), repair products with ceramide and collagen peptide as core ingredients have received widespread attention. Ceramide, also known as sphingolipids, is a lipid present in the skin and plays an important role in the formation of the epidermal stratum corneum. Recent studies have shown that when the skin becomes dry, flaky, and cracked, and its barrier function is significantly reduced, skin supplementation with ceramide can quickly restore moisturizing and barrier functions; collagen peptides can improve skin symptoms such as dullness, sagging, and wrinkles caused by excessive loss of collagen, and collagen tripeptide is the smallest and most stable structural unit of collagen. It is a tripeptide containing glycine, proline (or hydroxyproline) plus one other amino acid. Due to the small molecular weight of collagen tripeptide, after enzymatic hydrolysis, the human body absorption rate can reach 95%, and it can then effectively penetrate into the stratum corneum, dermis, and hair root cells.

[0003] Whey fermentation products are mainly made from whey powder, yeast extract, etc. They have the ability to scavenge harmful free radicals and inhibit lipid peroxidation. They can play an important role in the prevention and treatment of diseases, delaying the aging of the body, etc. At the same time, they can also obtain additional nutritional value and other functional properties. The development of whey fermentation products and related functional health foods has become one of the most promising industries in the food industry.

[0004] Chinese patent CN118489894A discloses an oral composition containing ceramide that can repair the skin barrier and its application. The invention includes rice bran oil powder, collagen peptide, vitamin E, double rose pollen and konjac powder. The rice bran oil powder containing ceramide is compounded with other raw materials to significantly increase the water content of the tester's skin and significantly reduce the water loss of the skin. It has a good repair effect on the damaged skin barrier, but due to the single type of ceramide, it is difficult to simulate the natural lipid diversity of the skin, resulting in an incomplete repair effect. Chinese patent CN109105684A discloses a composition for reducing the glycosylation of collagen peptides. The composition is applied to the field of food and health products. The invention has the effect of synergistically reducing the glycosylation of collagen peptides through Haematococcus pluvialis, cherry concentrate and pomegranate concentrate, and improves the utilization rate of collagen to enhance the effect of delaying skin aging. Therefore, the present invention provides a composition containing ceramide and collagen tripeptide and its preparation method, which is further optimized for the singleness of ceramide and improving the utilization rate of collagen. Summary of the invention

[0005] The present invention relates to a composition containing ceramide and collagen tripeptide and a preparation method thereof, and belongs to the technical field of health care products. The present invention discloses a composition containing ceramide and collagen tripeptide, which comprises the following raw materials: collagen tripeptide, rice bran oil powder, houttuynia cordata extract, pineapple powder, konjac powder, grape seed extract, olive fruit powder, carnosine, pomegranate concentrated powder and whey fermentation. Grape seed extract, olive fruit powder, carnosine and pomegranate concentrated powder inhibit the Maillard reaction of collagen peptides and improve the utilization rate of collagen tripeptide; ceramide rich in rice bran oil powder is a natural moisturizing factor for the skin, and is matched with pineapple powder and konjac powder having keratin metabolism to maintain the health of the stratum corneum; houttuynia cordata extract and whey fermentation have excellent anti-inflammatory effects, so the components enhance the skin barrier in multiple dimensions through collagen synthesis, stratum corneum maintenance, anti-inflammatory and anti-oxidation.

[0006] The purpose of the present invention can be achieved through the following technical solutions: A composition containing ceramide and collagen tripeptide comprises the following raw materials: collagen tripeptide, rice bran oil powder, houttuynia cordata extract, pineapple powder, konjac powder, grape seed extract, olive fruit powder, carnosine, pomegranate concentrated powder and whey fermentation product.

[0007] Furthermore, the composition containing ceramide and collagen tripeptide comprises the following raw materials in parts by weight: 150-200 parts of collagen tripeptide, 80-120 parts of rice bran oil powder, 3-5 parts of houttuynia cordata extract, 5-8 parts of pineapple powder, 4-6 parts of konjac flour, 2-4 parts of grape seed extract, 2-3 parts of olive fruit powder, 0.5-1 part of carnosine, 1-3 parts of pomegranate concentrated powder and 1-3 parts of whey fermentation.

[0008] Furthermore, the carnosine is one or more of anserine, bovine cardiomyocytes and deer cardiomyocytes.

[0009] Furthermore, the preparation method of the whey fermentation product comprises the following steps: (1) whey powder is mixed with distilled water, sterilized by heating, and then cooled to room temperature to obtain a mixed solution; (2) The mixed solution is inoculated with composite bacteria for fermentation under anaerobic conditions, and then heated and sterilized after fermentation to obtain whey fermentation product.

[0010] Furthermore, in the step (1), the mass ratio of whey powder to distilled water is 1-2:10, and the temperature and time of the heating sterilization are 80-100° C. and 30 min, respectively.

[0011] Furthermore, in step (2), the composite bacteria accounts for 2-3% of the mass of the mixed solution, wherein the composite bacteria is composed of Lactobacillus delbrueckii subspecies bulgaricus and Bifidobacterium longum subspecies longum in a mass ratio of 1:1-2.

[0012] Furthermore, in step (2), the fermentation temperature, pH and time are 38-40°C, 7-7.1 and 24h respectively, and the heating sterilization temperature and time are 60-65°C and 20-30min respectively.

[0013] Furthermore, the preparation method of the composition containing ceramide and collagen tripeptide is: compounding all raw materials according to weight parts.

[0014] Beneficial effects of the present invention: 1. The polyphenols and anthocyanins in the grape seed extract, olive fruit powder and pomegranate concentrated powder of the present invention have a synergistic antioxidant effect, jointly scavenging free radicals and reducing the Maillard reaction of collagen tripeptide, while the addition of carnosine enhances the capture of carbonyl groups and further inhibits the occurrence of Maillard reaction, thereby improving the utilization rate of collagen; 2. Lactobacillus delbrueckii subspecies bulgaricus and Bifidobacterium longum subspecies longum are used as composite bacteria to ferment whey protein. The active substances produced by the two strains increase the release of amino acids in whey protein, thereby obtaining highly nutritious whey fermentation products. At the same time, Houttuynia cordata extract is added to enhance the anti-inflammatory properties. 3. Collagen tripeptide has the function of synthesizing collagen, plays a supporting role in the dermis, gives the skin a certain thickness and elasticity, and protects skin lipids; rice bran oil powder and pineapple powder are rich in ceramide, and pineapple powder and konjac powder have the function of regulating keratin withering, maintaining the health of the stratum corneum; Houttuynia cordata extract and whey protein fermentation have excellent anti-inflammatory properties, and when combined with other antioxidant components, the composition prepared by the present invention enhances the skin barrier from multiple dimensions of collagen synthesis, stratum corneum maintenance, anti-inflammatory and anti-oxidation. DETAILED DESCRIPTION

[0015] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with embodiments.

[0016] The collagen tripeptide involved in the present invention was purchased from Shanxi Zhongnuo Biotechnology Co., Ltd.; rice bran oil powder was purchased from Leshengyuan Biotechnology (Nanjing) Co., Ltd.; houttuynia cordata extract was purchased from Shanyang Lianfeng Biotechnology Co., Ltd.; the mesh size of pineapple powder, konjac powder and olive fruit powder was 100 mesh; grape seed extract was purchased from Shanghai Zhongfeng Biotechnology Co., Ltd., with CAS No. 84929-27-1; pomegranate concentrated powder was purchased from Lanzhou Waters Biotechnology Co., Ltd.; whey powder was purchased from Shanghai Zhongfeng Biotechnology Co., Ltd.; anserine was purchased from Shandong Aicai Biotechnology Co., Ltd.; bovine myocardin was purchased from Xi'an Jiatian Biotechnology Co., Ltd.; deer myocardin was purchased from Zhengzhou Yuhe Food Additive Co., Ltd.; the preservation number of Lactobacillus delbrueckii subspecies bulgaricus was CGMCC1.16075; the preservation number of Bifidobacterium longum subspecies longum was CGMCC1.5052; the effective live bacterial counts of Lactobacillus delbrueckii subspecies bulgaricus and Bifidobacterium longum subspecies longum were both 1*10 6 CFU / mL.

[0017] Example 1 A composition containing ceramide and collagen tripeptide comprises the following raw materials: collagen tripeptide, rice bran oil powder, houttuynia cordata extract, pineapple powder, konjac powder, grape seed extract, olive fruit powder, carnosine, pomegranate concentrated powder and whey fermentation product.

[0018] The composition containing ceramide and collagen tripeptide comprises the following raw materials in parts by weight: 150 parts of collagen tripeptide, 80 parts of rice bran oil powder, 3 parts of houttuynia cordata extract, 5 parts of pineapple powder, 4 parts of konjac powder, 2 parts of grape seed extract, 2 parts of olive fruit powder, 0.5 parts of carnosine, 1 part of pomegranate concentrated powder and 1 part of whey fermentation product.

[0019] The carnosine is anserine.

[0020] The preparation method of the whey fermentation product comprises the following steps: (1) whey powder is mixed with distilled water, sterilized by heating, and then cooled to room temperature to obtain a mixed solution; (2) The mixed solution is inoculated with composite bacteria for fermentation under anaerobic conditions, and then heated and sterilized after fermentation to obtain whey fermentation product.

[0021] In the step (1), the mass ratio of whey powder to distilled water is 1:10, and the temperature and time of the heating sterilization are 80° C. and 30 min, respectively.

[0022] In the step (2), the composite bacteria accounts for 2% of the mass of the mixed solution, wherein the composite bacteria is composed of Lactobacillus delbrueckii subspecies bulgaricus and Bifidobacterium longum subspecies longum in a mass ratio of 1:1.

[0023] The fermentation temperature, pH and time in step (2) are 38° C., 7 and 24 h respectively, and the heating sterilization temperature and time are 60° C., 20 min respectively.

[0024] The preparation method of the composition containing ceramide and collagen tripeptide is as follows: all raw materials are compounded according to weight parts.

[0025] Example 2 A composition containing ceramide and collagen tripeptide comprises the following raw materials: collagen tripeptide, rice bran oil powder, houttuynia cordata extract, pineapple powder, konjac powder, grape seed extract, olive fruit powder, carnosine, pomegranate concentrated powder and whey fermentation product.

[0026] The composition containing ceramide and collagen tripeptide comprises the following raw materials in parts by weight: 180 parts of collagen tripeptide, 100 parts of rice bran oil powder, 4 parts of houttuynia cordata extract, 6.5 parts of pineapple powder, 5 parts of konjac powder, 3 parts of grape seed extract, 2.5 parts of olive fruit powder, 0.8 parts of carnosine, 2 parts of pomegranate concentrated powder and 2 parts of whey fermentation product.

[0027] The carnosine is composed of anserine and bovine myocardin in a mass ratio of 1:1.

[0028] The preparation method of the whey fermentation product comprises the following steps: (1) whey powder is mixed with distilled water, sterilized by heating, and then cooled to room temperature to obtain a mixed solution; (2) The mixed solution is inoculated with composite bacteria for fermentation under anaerobic conditions, and then heated and sterilized after fermentation to obtain whey fermentation product.

[0029] In the step (1), the mass ratio of whey powder to distilled water is 1.5:10, and the temperature and time of the heating sterilization are 90° C. and 30 min, respectively.

[0030] In the step (2), the composite bacteria accounts for 2.5% of the mass of the mixed solution, wherein the composite bacteria is composed of Lactobacillus delbrueckii subspecies bulgaricus and Bifidobacterium longum subspecies longum in a mass ratio of 1:1.5.

[0031] The temperature, pH and time of the fermentation in step (2) are 39° C., 7 and 24 h respectively, and the temperature and time of the heating sterilization are 63° C. and 25 min respectively.

[0032] The preparation method of the composition containing ceramide and collagen tripeptide is as follows: all raw materials are compounded according to weight parts.

[0033] Example 3 A composition containing ceramide and collagen tripeptide comprises the following raw materials: collagen tripeptide, rice bran oil powder, houttuynia cordata extract, pineapple powder, konjac powder, grape seed extract, olive fruit powder, carnosine, pomegranate concentrated powder and whey fermentation product.

[0034] The composition containing ceramide and collagen tripeptide comprises the following raw materials in parts by weight: 200 parts of collagen tripeptide, 120 parts of rice bran oil powder, 5 parts of houttuynia cordata extract, 8 parts of pineapple powder, 6 parts of konjac powder, 4 parts of grape seed extract, 3 parts of olive fruit powder, 1 part of carnosine, 3 parts of pomegranate concentrated powder and 3 parts of whey fermentation product.

[0035] The carnosine is composed of anserine, bovine cardiomyopeptide and deer cardiomyopeptide in a mass ratio of 1:1:1.

[0036] The preparation method of the whey fermentation product comprises the following steps: (1) whey powder is mixed with distilled water, sterilized by heating, and then cooled to room temperature to obtain a mixed solution; (2) The mixed solution is inoculated with composite bacteria for fermentation under anaerobic conditions, and then heated and sterilized after fermentation to obtain whey fermentation product.

[0037] In the step (1), the mass ratio of whey powder to distilled water is 2:10, and the temperature and time for the heating sterilization are 100° C. and 30 min, respectively.

[0038] In the step (2), the composite bacteria accounts for 3% of the mass of the mixed solution, wherein the composite bacteria is composed of Lactobacillus delbrueckii subspecies bulgaricus and Bifidobacterium longum subspecies longum in a mass ratio of 1:2.

[0039] The temperature, pH and time of the fermentation in step (2) are 40° C., 7.1 and 24 h respectively, and the temperature and time of the heating sterilization are 65° C. and 30 min respectively.

[0040] The preparation method of the composition containing ceramide and collagen tripeptide is as follows: all raw materials are compounded according to weight parts.

[0041] Comparative Example 1 On the basis of Example 2, the grape seed extract in the raw material was removed, and the reduced mass was distributed to the mass of carnosine, olive fruit powder and pomegranate concentrated powder in proportion. Other conditions were consistent with Example 2.

[0042] Comparative Example 2 On the basis of Example 2, the olive fruit powder in the raw material was removed, and the reduced mass was distributed to the masses of grape seed extract, carnosine and pomegranate concentrated powder in proportion. Other conditions were consistent with Example 2.

[0043] Comparative Example 3 On the basis of Example 2, the pomegranate concentrated powder was removed from the raw material, and the reduced mass was distributed to the masses of grape seed extract, olive fruit powder and carnosine in proportion. Other conditions were consistent with Example 2.

[0044] Comparative Example 4 On the basis of Example 2, carnosine was removed from the raw material, and the reduced mass was distributed to the masses of grape seed extract, olive fruit powder and pomegranate concentrated powder in proportion, and other conditions were consistent with Example 2.

[0045] Comparative Example 5 On the basis of Example 2, Lactobacillus delbrueckii subsp. bulgaricus in the composite bacteria was removed and replaced with Bifidobacterium longum subsp. longum of equal mass, and other conditions were consistent with Example 2.

[0046] Comparative Example 6 On the basis of Example 2, Bifidobacterium longum subsp. longum in the composite bacteria was removed and replaced with Lactobacillus delbrueckii subsp. bulgaricus of equal mass, and other conditions were consistent with Example 2.

[0047] Comparative Example 7 The only difference from Example 2 is that the mass ratio of Lactobacillus delbrueckii subsp. bulgaricus to Bifidobacterium longum subsp. longum in Comparative Example 7 is 1:3, and other conditions are consistent with Example 2.

[0048] Comparative Example 8 The only difference from Example 2 is that the mass ratio of Lactobacillus delbrueckii subsp. bulgaricus to Bifidobacterium longum subsp. longum in Comparative Example 8 is 2:1, and other conditions are consistent with Example 2.

[0049] Comparative Example 9 On the basis of Example 2, the Houttuynia cordata extract in the raw material was removed and replaced with an equal weight portion of whey protein fermentation product. Other conditions were consistent with Example 2.

[0050] Comparative Example 10 On the basis of Example 2, the whey protein fermentation product in the raw material was removed and replaced with an equal weight portion of Houttuynia cordata extract. Other conditions were the same as in Example 2.

[0051] Comparative Example 11 On the basis of Example 2, the pineapple powder in the raw material was removed and replaced with an equal weight portion of rice bran oil powder, and other conditions were consistent with Example 2.

[0052] Performance Testing The compositions containing ceramide and collagen tripeptide prepared in Examples 1-3 and Comparative Examples 1-11 were respectively prepared with purified water at a mass ratio of 1:80 to prepare samples; Animal toxicity test: 140 rats weighing 200±10g were selected and divided into 14 groups, with 10 rats in each group. The rats were fed normally and gavaged with the test samples for 7 consecutive days, with 2mL gavage per rat every day. The rats were observed every day to see if there was any difference in food intake and whether the rats had any adverse reactions. The test results are shown in Table 1.

[0053] Table 1 Test results

[0054] As can be seen from Table 1, the embodiments and comparative examples had no effect on the food intake of rats and no adverse reactions occurred.

[0055] Collagen tripeptide determination: The content of collagen tripeptide was determined by measuring the content of glycine and proline. According to the test method for glycine and proline provided in patent CN109105684A, the content of glycine and proline was tested for Examples 1-3 and Comparative Examples 1-4. In addition, the beverage with reduced glycosylation of collagen peptides prepared according to Example 2 in patent CN109105684A was used as control group 2, and the test of collagen tripeptide was used as control group 1. The mass of the test samples in each group was equal; Table 2 Test results

[0056] Note: Compared with the control group 1, the examples, comparative examples 1-4, and control group 2: "*" means P < 0.05 with significant difference, "**" means P < 0.01 with very significant difference; compared with the examples, comparative examples 1-4 and control group 2: "◊" means P > 0.05 with no significant difference, "▷" means P < 0.05 with significant difference.

[0057] From the analysis of Table 2, it can be concluded that the contents of glycine and proline in Example and Control Group 2 were significantly different from those in Control Group 1 (P < 0.01), and the contents of glycine and proline in Comparative Examples 1-4 were significantly different from those in Control Group 1 (P < 0.05); there were no significant differences in the contents of glycine and proline between Example and Control Group 2 (P > 0.05), there were no significant differences in the contents of glycine between Comparative Examples 1-3 and Control Group 2 (P > 0.05), there were significant differences in the contents of glycine between Comparative Examples 4 and Control Group 2 (P < 0.05), and there were significant differences in the contents of proline between Comparative Examples 1-4 and Control Group 2 (P < 0.05). Through data analysis, it can be concluded that if any one of the grape seed extract, carnosine, olive fruit powder and pomegranate concentrated powder is removed from the raw materials, the collagen tripeptide will be consumed by the Maillard reaction, and the effect is greatest when carnosine is removed. In addition, it can be concluded that the Example is more effective in inhibiting the Maillard reaction of collagen peptides than the control group 2, because carnosine in Example 2 enhances the effect of capturing carbonyl groups, thereby improving the utilization of collagen tripeptide.

[0058] Anti-inflammatory test: Inflammation in the skin is mainly caused by the irritation of dust in the air. The compositions containing ceramide and collagen tripeptide prepared in Examples 1-3 and Comparative Examples 5-10 were respectively prepared into samples with a mass ratio of 1:80 with purified water. The anti-inflammatory effect was obtained by testing the effectiveness of the samples in inhibiting the secretion of inflammatory factors IL-8 and TNF-α. Specifically, 9 groups were inoculated, and normal human epidermal keratinocytes were inoculated into a 96-well plate at a density of 1E4 cells / well in each group, and incubated overnight in an incubator (37°C, 5% CO2, 95% RH); When the cell plating rate in the 96-well plate reaches 50%, each group is respectively added with a 3% volume ratio of the sample, and group stimulation is performed, and 0.1 mg / mL of air dust is added to each well (an additional group of samples in Example 2 is set as a blank control group, and the control wells are not added with air dust), and each group is set with 3 duplicate wells. Continue to culture in an incubator (37°C, 5% CO2, 95% RH), and after culturing for 24 hours, collect the cell culture supernatant in an EP tube, and place it in a -80°C refrigerator for freezing and storage after collection, and then analyze the inhibition of IL-8 and TNF-α of each group by ELISA detection, and the results are shown in Table 3.

[0059] Table 3

[0060] It can be concluded from the data in Table 3 that the anti-inflammatory effects of Examples 1-3 are better than those of Comparative Examples 5-10, among which Comparative Example 9 has the worst anti-inflammatory effect. Due to the lack of Houttuynia cordata extract in Comparative Example 9, the composition lacks the main anti-inflammatory components, and the inhibition rate of inflammatory factors is greatly reduced; in Comparative Example 10, no whey fermentation product was added, and the inhibition rate of inflammatory factors decreased accordingly, because whey fermentation is rich in various amino acids, which enhances the anti-inflammatory effect of Houttuynia cordata, while Comparative Examples 5-6 are fermented by a single strain, which reduces the activity of active ingredients and substances in the fermentation process. The ratio between the composite bacteria in Comparative Examples 6-8 exceeds the scope of the embodiments. Therefore, Comparative Examples 5-8 affect the full release of the nutrients of the whey fermentation product, and thus the inhibition rate of inflammatory factors is reduced.

[0061] Skin stratum corneum moisture content test: The compositions containing ceramide and collagen tripeptide prepared in Examples 1-3, Comparative Examples 1-4 and Comparative Example 11 were respectively prepared into samples with pure water at a mass ratio of 1:80. The samples were tested according to the determination of moisture content in the stratum corneum of the skin in patent CN118489894A. Each volunteer took 5 mL of the sample per day. The test results are shown in Table 4.

[0062] Table 4

[0063] As can be seen from Table 4, the skin moisture content of Examples 1-3 after use is much greater than that of Comparative Examples 1-4 and Comparative Example 11, among which the skin moisture content of Comparative Example 11 is the lowest. Since pineapple powder is removed in Comparative Example 11, the single ceramide component is difficult to simulate the natural lipid diversity of the skin, resulting in poor skin water-locking effect; Comparative Examples 1-4 lack any one of grape seed extract, carnosine, olive fruit powder and pomegranate concentrated powder, which reduces the synthesis of collagen, thereby affecting the moisturizing effect of the skin and thus affecting the barrier function of the skin.

[0064] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A composition containing ceramide and collagen tripeptide, characterized in that: The composition containing ceramide and collagen tripeptide comprises the following raw materials: collagen tripeptide, rice bran oil powder, houttuynia cordata extract, pineapple powder, konjac flour, grape seed extract, olive fruit powder, carnosine, pomegranate concentrated powder and whey fermentation product. The raw materials of the composition are as follows in weight: 150-200 parts of collagen tripeptide, 80-120 parts of rice bran oil powder, 3-5 parts of houttuynia cordata extract, 5-8 parts of pineapple powder, 4-6 parts of konjac flour, 2-4 parts of grape seed extract, 2-3 parts of olive fruit powder, 0.5-1 part of carnosine, 1-3 parts of pomegranate concentrated powder and 1-3 parts of whey fermentation product.

2. A composition containing ceramide and collagen tripeptide according to claim 1, characterized in that: The carnosine is one or more of anserine, bovine cardiomyopeptide and deer cardiomyopeptide.

3. A composition containing ceramide and collagen tripeptide according to claim 1, characterized in that: The preparation method of the whey fermentation product comprises the following steps: (1) whey powder is mixed with distilled water, sterilized by heating, and then cooled to room temperature to obtain a mixed solution; (2) The mixed solution is inoculated with composite bacteria for fermentation under anaerobic conditions, and then heated and sterilized after fermentation to obtain whey fermentation product.

4. A composition containing ceramide and collagen tripeptide according to claim 3, characterized in that: In the step (1), the mass ratio of whey powder to distilled water is 1-2:10, and the temperature and time of the heating sterilization are 80-100° C. and 30 min, respectively.

5. A composition containing ceramide and collagen tripeptide according to claim 3, characterized in that: In the step (2), the composite bacteria accounts for 2-3% of the mass of the mixed solution, wherein the composite bacteria is composed of Lactobacillus delbrueckii subspecies bulgaricus and Bifidobacterium longum subspecies longum in a mass ratio of 1:1-2.

6. A composition containing ceramide and collagen tripeptide according to claim 3, characterized in that: The temperature, pH and time of the fermentation in step (2) are 38-40°C, 7-7.1 and 24h respectively, and the temperature and time of the heating sterilization are 60-65°C and 20-30min respectively.

7. A composition containing ceramide and collagen tripeptide according to claim 1, characterized in that: The preparation method of the composition containing ceramide and collagen tripeptide is as follows: all raw materials are compounded according to weight parts.

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