Honeysuckle mouth wash with bacteriostatic effect and preparation method thereof

Abstract

The invention provides honeysuckle mouth wash with a bacteriostatic effect and a preparation method of the honeysuckle mouth wash. According to the mouthwash provided by the invention, various plant raw materials are adopted, and the treated honeysuckle recycled water is added, so that the reutilization of resources is realized, more effective components are provided, and the mouthwash has the advantages of energy conservation and environmental protection. Wherein the honeysuckle, the dandelion and the liquorice are traditional medicinal and edible traditional Chinese medicines with the effects of clearing away heat and toxic materials, inhibiting bacteria and diminishing inflammation and the like, and are safe and effective; the traditional Chinese medicine has a certain inhibition effect on staphylococcus aureus and escherichia coli. The preparation method is simple, the process is stable, large-scale production can be achieved, and the toothpaste can refresh breath, inhibit bacteria and resist inflammation and is convenient to carry and use. The prepared honeysuckle mouth wash has a certain plant fragrance, is clear, free of impurities and good in heat resistance, cold resistance and stability, has the pH value of 6.65-7.01, and meets the industrial standard product quality requirements.

Description

Technical Field

[0001] The invention belongs to the field of drug preparation, and in particular relates to a honeysuckle mouthwash with antibacterial effect and a preparation method thereof. Background Art

[0002] According to the Global Report on Oral Health, nearly 3.5 billion people in the world may have a variety of oral problems such as caries, loose teeth, and bad breath. With the intensification of urbanization and changes in people's living environment, the prevalence rate has shown an upward trend year by year. It can be seen that oral problems are imminent and the public's awareness of oral health needs to be improved. Among many oral care products, mouthwash has gradually stood out in the prevention and auxiliary treatment of oral diseases with its strong fluidity, convenience and effectiveness, and plays an important role in maintaining oral health. However, most of the mouthwashes on the market contain chemical bactericides such as antibiotics. Long-term use may cause oral flora imbalance and damage to oral mucosa. Honeysuckle, as a traditional Chinese medicine with the same origin of medicine and food, has the effects of clearing heat and detoxifying, evacuating wind and heat, and is widely used in clinical practice. Therefore, this project intends to use honeysuckle as the main ingredient, and add dandelion, licorice and other Chinese medicines with the same origin of medicine and food and the effect of clearing heat and detoxifying, to enhance its heat-clearing and detoxifying effect and improve its antibacterial ability.

[0003] Lonicera japonica Lonicera japonica The dried buds or flowers of Thunb. have the effects of clearing away heat and detoxifying, dispersing wind-heat, and are used to treat carbuncle, furuncle, throat paralysis, erysipelas, dysentery caused by heat toxins, wind-heat cold, fever caused by febrile diseases, etc. Modern research shows that honeysuckle contains a variety of chemical components such as volatile oils, organic acids, flavonoids, and triterpenoid saponins, among which chlorogenic acid, isochlorogenic acid and its flavonoid compounds are the main antibacterial components. The in vitro antibacterial activity study of honeysuckle extract showed that honeysuckle decoction showed a certain inhibitory effect on Staphylococcus aureus, Escherichia coli, Candida albicans, etc. Another study by Palíková et al. showed that honeysuckle extract can reduce the biofilm formation and adhesion of Candida, Staphylococcus epidermidis, Escherichia coli, Enterococcus faecalis and Streptococcus mutans.

[0004] As a byproduct produced during the drying process of fresh honeysuckle, honeysuckle recycled water is often directly discarded and fails to be effectively developed and reused. The present invention finds through research on honeysuckle recycled water that the recycled water has a fresh honeysuckle fragrance, contains a variety of effective chemical components with pharmacological activity, and the content of isochlorogenic acid B, a unique component of honeysuckle, is much higher than that of dry honeysuckle. Therefore, research on the reuse of honeysuckle recycled water is of great significance. It can be added to mouthwash to increase its effective ingredient content and honeysuckle fragrance. Summary of the invention

[0005] The invention aims to provide a honeysuckle mouthwash containing honeysuckle recycled water and having antibacterial effect and a preparation method thereof.

[0006] In order to achieve the purpose of the present invention, in a first aspect, the present invention provides a honeysuckle mouthwash with antibacterial effect, the mouthwash consisting of a traditional Chinese medicine extract, honeysuckle recovered water and a mouthwash base, wherein the weight percentage of the traditional Chinese medicine extract is 5-20%, the weight percentage of the honeysuckle dried recovered water is 5-40%, and the remainder is the mouthwash base.

[0007] The Chinese medicine formula is composed of three medicines: honeysuckle, dandelion and liquorice. Honeysuckle is used as the main medicine in the prescription. Modern studies have shown that honeysuckle is rich in active ingredients such as chlorogenic acid and flavonoids, has significant antibacterial effects, can effectively inhibit the growth of various bacteria, and honeysuckle also has the effects of clearing away heat and detoxicating, evacuating wind-heat, and laying the foundation for antibacterial and anti-inflammatory for the compound. At the same time, dandelion and mint are used as ministerial medicines, both of which have strong antibacterial ability, and match with honeysuckle, which can enhance its heat-clearing and detoxicating, antibacterial and anti-inflammatory effects. And liquorice is used as the guiding medicine, which not only has heat-clearing and detoxicating, antibacterial and anti-inflammatory effects, but also can reconcile various medicines and improve the overall efficacy. The four medicines are used together, which can not only effectively inhibit the harmful flora in the oral cavity, reduce oral inflammation, but also freshen the breath, and achieve a comprehensive oral care effect.

[0008] The Chinese medicine extract is prepared by mixing honeysuckle, dandelion and licorice in a weight ratio of (8-1:6-1:4-1) and extracting with water; preferably, the weight ratio of honeysuckle, dandelion and licorice is 2:1:1.

[0009] The honeysuckle recycled water is condensed water obtained by drying fresh honeysuckle in a sealed dryer, and is recovered after filtering and sterilization; preferably, the weight percentage of the honeysuckle recycled water in the honeysuckle mouthwash is 10-30%.

[0010] The mouthwash base is composed of the following ingredients in percentage by weight: 0.01-1% thymol, 0.01-0.3% plant essential oil, 0.01-1% cooling agent, 2-15% wetting agent, 0.04-1% sweetener, 0.1%-2% surfactant, 0.01-0.5% preservative, and the balance is water (total percentage by weight is 100%).

[0011] Furthermore, the wetting agent is selected from one or more of glycerol, propylene glycol, sorbitol or ethanol.

[0012] Furthermore, the solubilizer is selected from one or more of propylene glycol, ethanol, polysorbate 20, glycerol, polyethylene glycol, and the like.

[0013] Furthermore, the surfactant includes but is not limited to poloxamer 407, polysorbate 20, polyoxyethylene (20) castor oil, and Tween.

[0014] Furthermore, the sweetener is selected from steviol glycosides and / or mogrosides and / or erythritol and the like.

[0015] Furthermore, the cooling agent is selected from WS-3 and / or WS-23 and / or menthol and the like.

[0016] Furthermore, the plant essential oil is selected from one of honeysuckle essential oil, peppermint essential oil or sweet orange essential oil.

[0017] Furthermore, the preservative is selected from methyl parahydroxybenzoate and / or propyl parahydroxybenzoate and / or sodium benzoate, etc.

[0018] In a second aspect, the present invention provides a method for preparing the mouthwash, comprising the following steps: (1) Weigh the coarse powder of honeysuckle, dandelion and liquorice respectively, and heat and reflux the mixture with water 10 to 40 times of their weight; (2) Preliminarily filtering the extracted medicinal solution under reduced pressure until no impurities are precipitated, and then further adsorbing and filtering the pigments and impurities with an activated carbon filter to obtain a clarified medicinal solution, i.e., a Chinese medicine extract, as solution A; (3) Preliminary filtration and ultraviolet sterilization of the collected honeysuckle recycled water to obtain solution B; (4) According to the formula requirements of the mouthwash base, various water-insoluble ingredients (such as plant essential oils, cooling agents, thymol, etc.) are dissolved in a certain amount of wetting agent and mixed evenly to form solution C; (5) Then, the mixed solution A and solution C are added to the remaining drinking water under heating conditions, and solution B, preservatives, sweeteners and other ingredients are added and mixed evenly to prepare honeysuckle mouthwash.

[0019] Furthermore, in step (1), the temperature of the heating reflux extraction is 75-95° C., and the extraction time is 40-90 minutes.

[0020] Furthermore, in step (3), the honeysuckle recycled water is collected by drying fresh honeysuckle in a sealed dryer at 40-80° C. for 1-48 hours.

[0021] Furthermore, in step (5), the heating temperature is 50-70° C., and the solution is stirred while heating until the solution is evenly mixed.

[0022] The pharmacologically active chemical components contained in the honeysuckle recycled water of the present invention are shown in Table 1-1 and Table 1-2.

[0023] Table 1-1 Chemical composition of honeysuckle recycled water

[0024] Table 1-2 Chemical composition of honeysuckle recycled water

[0025] By means of the above technical solution, the present invention has at least the following advantages and beneficial effects: The mouthwash provided by the present invention uses a variety of plant raw materials, among which honeysuckle, dandelion and liquorice are all traditional Chinese medicines for both medicinal and edible use, which have the effects of clearing away heat and detoxifying, inhibiting bacteria and reducing inflammation, etc., and are safe and effective; and the Chinese medicines all have a certain inhibitory effect on Staphylococcus aureus and Escherichia coli. Figure 1 shown.

[0026] The honeysuckle recycled water provided by the present invention is the reuse of honeysuckle resources, and the recycled water has a variety of chemical components containing pharmacological activity and has certain antibacterial and anti-inflammatory activities, as shown in Table 1-1 and Table 1-2.

[0027] The auxiliary materials used have a simple preparation method, a stable process, can be mass-produced, can freshen breath, inhibit bacteria and fight inflammation, and are easy to carry around for use.

[0028] The prepared honeysuckle mouthwash has a certain plant aroma, is clear and free of impurities, has good heat and cold resistance and stability, has a pH value of 6.65-7.01, and meets the industry standard product quality requirements. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 The antibacterial zones of the Chinese medicine extracts in the preferred embodiments of the present invention are shown in Figure 1. a: liquorice extract, b: honeysuckle extract, c: (honeysuckle + liquorice + dandelion) extract, d: dandelion extract, e: ethanol (negative control), and f: distilled water (negative control).

[0030] Figure 2 These are the essential oil inhibition zones in the preferred embodiments of the present invention: a: honeysuckle essential oil, b: peppermint oil, c: thymol, d: sweet orange essential oil.

[0031] Figure 3This is the antibacterial activity test of honeysuckle mouthwash in the preferred embodiment of the present invention. a: Escherichia coli + mouthwash (parallel control 1), b: Escherichia coli + mouthwash (parallel control 2), c: positive control (Escherichia coli), d: negative control (0.9% saline), e: Staphylococcus aureus + mouthwash (parallel control 1), f: Staphylococcus aureus + mouthwash (parallel control 2), g: positive control (Staphylococcus aureus), h: negative control (0.9% saline), i: Candida albicans + mouthwash (parallel control 1), j: Candida albicans + mouthwash (parallel control 2), k: positive control (Candida albicans), l: negative control (0.9% saline). DETAILED DESCRIPTION

[0032] Aiming at the problems that long-term use of antibacterial mouthwash in the prior art easily causes oral flora imbalance and damages oral mucosa, the present invention provides a honeysuckle mouthwash with antibacterial effect and a preparation method thereof.

[0033] The present invention adopts the following technical solution: The invention provides a honeysuckle mouthwash with antibacterial effect and a preparation method thereof. The mouthwash is prepared from a compound Chinese medicine extract consisting of honeysuckle, dandelion and liquorice in a certain proportion, honeysuckle recovered water and a mouthwash matrix; the weight parts of each raw material of the Chinese medicine extract are: 200-25 parts of honeysuckle, 150-25 parts of dandelion and 100-25 parts of liquorice.

[0034] Preferably, the weight ratio of the honeysuckle mouthwash is: 50 parts of honeysuckle, 25 parts of dandelions, and 25 parts of liquorice.

[0035] Preferably, the weight percentage of the extract is 5-20%, the weight percentage of honeysuckle recovered water is 5-40%, and the remainder is mouthwash base.

[0036] Preferably, the honeysuckle recycled water is condensed water obtained by drying fresh honeysuckle in a sealed dryer, and recovered through filtration and sterilization.

[0037] Preferably, the weight percentage of the honeysuckle recovered water is 10-30%.

[0038] Preferably, the weight percentage of the mouthwash matrix composition is: 0.01-0.1% thymol, 0.01-0.3% plant essential oil, 0.01-0.1% cooling agent, 2-15% wetting agent, 0.04-1% sweetener, 0.1%-2% surfactant, 0.01-0.5% preservative, and the balance is water.

[0039] Preferably, the wetting agent is one or more of glycerol, propylene glycol or ethanol, the surfactant is poloxamer 407, the sweetener is steviol glycoside or mogroside, the cooling agent is WS-3 or WS-23, the plant essential oil is one of honeysuckle essential oil, peppermint essential oil or sweet orange essential oil, and the preservative is one or more of methylparaben or propylparaben.

[0040] The method for preparing a honeysuckle mouthwash with antibacterial effect of the present invention comprises the following steps: (1) Weigh the coarse powder of honeysuckle, dandelion and liquorice respectively, and heat and reflux the mixture with 10 to 40 times the weight of water.

[0041] (2) The extracted drug solution is initially filtered under reduced pressure until no impurities are precipitated, and then further adsorbed and filtered with an activated carbon filter to remove pigments and impurities, so as to obtain a clarified drug solution as solution A.

[0042] (3) The collected honeysuckle recycled water is subjected to preliminary filtration and ultraviolet sterilization to obtain solution B.

[0043] (4) According to the formula requirements, the plant essential oil, cooling agent, thymol and other water-insoluble ingredients are dissolved in a certain amount of wetting agent and mixed evenly to form solution C.

[0044] (5) Then, the mixed solution A and solution C are added to the remaining drinking water under heating conditions, and solution B, preservatives, sweeteners and other ingredients are added and mixed evenly to prepare honeysuckle mouthwash.

[0045] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0046] Example 1

[0047] A Chinese medicine compound mouthwash comprises the following raw materials in parts by weight: 10% Chinese herbal medicine extract, 0.03% peppermint essential oil, 0.01% WS-3, 8% glycerol, 0.2% stevioside, 0.3% poloxamer 407, 0.2% methylparaben, 20% honeysuckle recycled water, and the rest is water. Among them, the raw materials of Chinese herbal medicine extract include: 50 parts of honeysuckle, 25 parts of dandelion, and 25 parts of licorice.

[0048] The preparation method of the Chinese medicine compound mouthwash comprises the following steps: First, weigh honeysuckle, dandelion, and liquorice powder respectively, and heat the mixture of traditional Chinese medicine with 20 times the weight of water for extraction. Perform preliminary decompression filtration on the extracted liquid, filter until there is no impurity precipitation, and then use an activated carbon filter to further adsorb and filter the pigments and impurities to obtain a clear liquid. According to the formula requirements, dissolve the water-insoluble ingredients such as peppermint essential oil and WS-3 in a certain amount of propylene glycol, mix evenly, and add to the clear liquid, add glycerin, stevioside, methylparaben, honeysuckle recycled water and water and mix thoroughly. Dispense and obtain.

[0049] Example 2

[0050] A Chinese medicine compound mouthwash comprises the following raw materials in parts by weight: 10% Chinese herbal medicine extract, 0.02% thymol, 0.03% sweet orange essential oil, 0.01% WS-3, 8% glycerol, 0.2% stevioside, 0.3% poloxamer 407, 0.2% methylparaben, 25% honeysuckle recycled water, and the rest is water. Among them, the raw materials of Chinese herbal medicine extract include: 50 parts of honeysuckle, 25 parts of dandelion, and 25 parts of licorice.

[0051] The preparation method of the Chinese medicine compound mouthwash comprises the following steps: First, weigh honeysuckle, dandelion, and liquorice powder respectively, and heat the mixture of traditional Chinese medicine with 20 times the weight of water for reflux extraction. Perform preliminary decompression filtration on the extracted liquid, filter until there is no impurity precipitation, and then use an activated carbon filter to further adsorb and filter the pigments and impurities to obtain a clear liquid. According to the formula requirements, dissolve the water-insoluble ingredients such as sweet orange essential oil, WS-3, and thymol in a certain amount of propylene glycol, mix evenly, and add to the clear liquid, add glycerin, stevioside, methylparaben, and honeysuckle recycled water and water and mix thoroughly. Dispense and obtain.

[0052] Example 3

[0053] A Chinese medicine compound mouthwash comprises the following raw materials in parts by weight: 10% Chinese herbal medicine extract, 0.02% thymol, 0.03% honeysuckle essential oil, 0.01% WS-3, 8% glycerol, 0.2% stevioside, 0.3% poloxamer 407, 0.2% methylparaben, 20% honeysuckle recycled water, and the rest is water. Among them, the raw materials of Chinese herbal medicine extract include: 50 parts of honeysuckle, 25 parts of dandelion, and 25 parts of licorice.

[0054] The preparation method of the Chinese medicine compound mouthwash comprises the following steps: First, weigh honeysuckle, dandelion, and liquorice powder respectively, and heat the mixture of traditional Chinese medicine with 20 times the weight of water for extraction. Perform preliminary decompression filtration on the extracted liquid, filter until there is no impurity precipitation, and then use an activated carbon filter to further adsorb and filter the pigments and impurities to obtain a clear liquid. According to the formula requirements, the water-insoluble ingredients such as honeysuckle essential oil, WS-3, and thymol are dissolved in a certain amount of propylene glycol in turn, mixed evenly, and added to the clear liquid, and glycerin, stevioside, methylparaben, honeysuckle recycled water and water are added and mixed thoroughly. Dispense and obtain.

[0055] Example 4

[0056] A Chinese medicine compound mouthwash comprises the following raw materials in parts by weight: 10% Chinese herbal medicine extract, 0.05% thymol, 0.03% peppermint essential oil, 0.01% WS-3, 8% glycerol, 0.2% stevioside, 0.3% poloxamer 407, 0.2% methylparaben, 15% honeysuckle recycled water, and the rest is water. Among them, the raw materials of Chinese herbal medicine extract include: 50 parts of honeysuckle, 25 parts of dandelion, and 25 parts of licorice.

[0057] The preparation method of the Chinese medicine compound mouthwash comprises the following steps: First, weigh honeysuckle, dandelion, and liquorice powder respectively, and heat the mixture of traditional Chinese medicine with 20 times the weight of water for extraction. Perform preliminary decompression filtration on the extracted liquid, filter until there is no impurity precipitation, and then use an activated carbon filter to further adsorb and filter the pigments and impurities to obtain a clear liquid. According to the formula requirements, dissolve the water-insoluble ingredients such as peppermint essential oil, WS-3, and thymol in a certain amount of propylene glycol, mix evenly, and add to the clear liquid, add glycerin, stevioside, methylparaben, and honeysuckle recycled water and water and mix thoroughly. Dispense and obtain.

[0058] Example 5

[0059] A Chinese medicine compound mouthwash comprises the following raw materials in parts by weight: 5% Chinese herbal medicine extract, 0.03% peppermint essential oil, 0.03% WS-3, 8% glycerol, 0.2% stevioside, 0.3% poloxamer 407, 0.2% methylparaben, 5% honeysuckle recycled water, and the rest is water. Among them, the raw materials of Chinese herbal medicine extract include: 50 parts of honeysuckle, 25 parts of dandelion, and 25 parts of licorice.

[0060] The preparation method of the Chinese medicine compound mouthwash comprises the following steps: First, weigh honeysuckle, dandelion, and liquorice powder respectively, and heat the mixture of traditional Chinese medicine with 20 times the weight of water for extraction. Perform preliminary decompression filtration on the extracted liquid, filter until there is no impurity precipitation, and then use an activated carbon filter to further adsorb and filter the pigments and impurities to obtain a clear liquid. According to the formula requirements, dissolve the water-insoluble ingredients such as peppermint essential oil and WS-3 in a certain amount of propylene glycol, mix evenly, and add to the clear liquid, add glycerin, stevioside, methylparaben, honeysuckle recycled water and water and mix thoroughly. Dispense and obtain.

[0061] Example 6

[0062] A Chinese medicine compound mouthwash comprises the following raw materials in parts by weight: 5% Chinese herbal medicine extract, 0.05% thymol, 0.03% peppermint essential oil, 0.03% WS-3, 8% glycerol, 0.2% stevioside, 0.3% poloxamer 407, 0.2% methylparaben, 20% honeysuckle recycled water, and the rest is water. Among them, the raw materials of Chinese herbal medicine extract include: 50 parts of honeysuckle, 25 parts of dandelion, and 25 parts of licorice.

[0063] The preparation method of the Chinese medicine compound mouthwash comprises the following steps: First, weigh honeysuckle, dandelion, and liquorice powder respectively, and heat the mixture of traditional Chinese medicine with 20 times the weight of water for extraction. Perform preliminary decompression filtration on the extracted liquid, filter until there is no impurity precipitation, and then use an activated carbon filter to further adsorb and filter the pigments and impurities to obtain a clear liquid. According to the formula requirements, dissolve the water-insoluble ingredients such as peppermint essential oil, WS-3, and thymol in a certain amount of propylene glycol, mix evenly, and add to the clear liquid, add glycerin, stevioside, methylparaben, and honeysuckle recycled water and water and mix thoroughly. Dispense and obtain.

[0064] Example 7

[0065] A Chinese medicine compound mouthwash comprises the following raw materials in parts by weight: 20% Chinese herbal medicine extract, 0.05% thymol, 0.03% peppermint essential oil, 0.03% WS-3, 8% glycerol, 0.2% stevioside, 0.3% poloxamer 407, 0.2% methylparaben, 20% honeysuckle recycled water, and the rest is water. Among them, the raw materials of Chinese herbal medicine extract include: 50 parts of honeysuckle, 25 parts of dandelion, and 25 parts of licorice.

[0066] The preparation method of the Chinese medicine compound mouthwash comprises the following steps: First, weigh honeysuckle, dandelion, and liquorice powder respectively, and heat the mixture of traditional Chinese medicine with 20 times the weight of water for extraction. Perform preliminary decompression filtration on the extracted liquid, filter until there is no impurity precipitation, and then use an activated carbon filter to further adsorb and filter the pigments and impurities to obtain a clear liquid. According to the formula requirements, dissolve the water-insoluble ingredients such as peppermint essential oil, WS-3, and thymol in a certain amount of propylene glycol, mix evenly, and add to the clear liquid, add glycerin, stevioside, methylparaben, and honeysuckle recycled water and water and mix thoroughly. Dispense and obtain.

[0067] Example 8

[0068] A Chinese medicine compound mouthwash comprises the following raw materials in parts by weight: 10% Chinese herbal medicine extract, 0.1% thymol, 0.03% peppermint essential oil, 0.03% WS-3, 8% glycerol, 0.2% stevioside, 0.3% poloxamer 407, 0.2% methylparaben, 30% honeysuckle recycled water, and the rest is water. Among them, the raw materials of Chinese herbal medicine extract include: 50 parts of honeysuckle, 25 parts of dandelion, and 25 parts of licorice.

[0069] The preparation method of the Chinese medicine compound mouthwash comprises the following steps: First, weigh honeysuckle, dandelion, and liquorice powder respectively, and heat the mixture of traditional Chinese medicine with 20 times the weight of water for extraction. Perform preliminary decompression filtration on the extracted liquid, filter until there is no impurity precipitation, and then use an activated carbon filter to further adsorb and filter the pigments and impurities to obtain a clear liquid. According to the formula requirements, dissolve the water-insoluble ingredients such as peppermint essential oil, WS-3, and thymol in a certain amount of propylene glycol, mix evenly, and add to the clear liquid, add glycerin, stevioside, methylparaben, and honeysuckle recycled water and water and mix thoroughly. Dispense and obtain.

[0070] Example 9

[0071] A Chinese medicine compound mouthwash comprises the following raw materials in parts by weight: 20% Chinese herbal medicine extract, 0.03% peppermint essential oil, 0.03% WS-3, 8% glycerol, 0.2% stevioside, 0.3% poloxamer 407, 0.2% methylparaben, 10% honeysuckle recycled water, and the rest is water. Among them, the raw materials of Chinese herbal medicine extract include: 50 parts of honeysuckle, 25 parts of dandelion, and 25 parts of licorice.

[0072] The preparation method of the Chinese medicine compound mouthwash comprises the following steps: First, weigh honeysuckle, dandelion, and liquorice powder respectively, and heat the mixture of traditional Chinese medicine with 20 times the weight of water for extraction. Perform preliminary decompression filtration on the extracted liquid, filter until there is no impurity precipitation, and then use an activated carbon filter to further adsorb and filter the pigments and impurities to obtain a clear liquid. According to the formula requirements, dissolve the water-insoluble ingredients such as peppermint essential oil and WS-3 in a certain amount of propylene glycol, mix evenly, and add to the clear liquid, add glycerin, stevioside, methylparaben, honeysuckle recycled water and water and mix thoroughly. Dispense and obtain.

[0073] Example 10

[0074] A Chinese medicine compound mouthwash comprises the following raw materials in parts by weight: 20% Chinese herbal medicine extract, 0.02% thymol, 0.03% peppermint essential oil, 0.03% WS-3, 8% glycerol, 0.2% stevioside, 0.3% poloxamer 407, 0.2% methylparaben, 10% honeysuckle recycled water, and the rest is water. Among them, the raw materials of Chinese herbal medicine extract include: 50 parts of honeysuckle, 25 parts of dandelion, and 25 parts of licorice.

[0075] The preparation method of the Chinese medicine compound mouthwash comprises the following steps: First, weigh honeysuckle, dandelion, and liquorice powder respectively, and heat the mixture of traditional Chinese medicine with 20 times the weight of water for extraction. Perform preliminary decompression filtration on the extracted liquid, filter until there is no impurity precipitation, and then use an activated carbon filter to further adsorb and filter the pigments and impurities to obtain a clear liquid. According to the formula requirements, dissolve the water-insoluble ingredients such as peppermint essential oil, WS-3, and thymol in a certain amount of propylene glycol, mix evenly, and add to the clear liquid, add glycerin, stevioside, methylparaben, and honeysuckle recycled water and water and mix thoroughly. Dispense and obtain.

[0076] Quality testing of mouthwash: 1. For reference of sensory, physical and chemical indicators of mouthwash, please see Table 2.

[0077] Table 2 Sensory and physical and chemical index reference of mouthwash

[0078] 2. Fragrance

[0079] Olfactory Identification

[0080] 3. Clarity

[0081] At room temperature and natural light, pour 10 mL of sample into a colorimetric tube and observe visually at a distance of 30 cm.

[0082] 4. Stability

[0083] (1) Heat resistance and stability

[0084] Pour 10 mL of the sample into a stoppered colorimetric tube and place it in a constant temperature incubator at (40±1)℃ for 24 hours.

[0085] Take out, return to room temperature, and observe visually.

[0086] (2) Cold resistance and stability

[0087] Pour 10 mL of the sample into a stoppered colorimetric tube, place it in a refrigerator at 0℃-4℃ for 24 hours, then take it out, return it to room temperature, and observe it visually.

[0088] 5. pH value

[0089] 20 mL of the sample was directly placed in a 50 mL beaker to immerse the electrode, and measured at 25°C using a pH meter.

[0090] 6. Detection of total bacterial colony count

[0091] Under sterile conditions, sample 100 μL of mouthwash and place it in a centrifuge tube containing 900 μL of ultrapure water to make a 1:10 uniform dilution. Use a sterile cotton swab to apply the dilution to each agar plate. After the sample is cultured at 37°C for 48 hours, the colony count is calculated. The total colony count per milliliter (CFU / mL) is less than or equal to 500 (CFU / mL) to be qualified.

[0092] Table 3 Test results of sensory and physical and chemical indicators of mouthwash

[0093] According to Table 3, all parameters of the mouthwashes prepared in Examples 1-9 meet the standard requirements of mouthwashes in the light industry.

[0094] 7. Antibacterial test

[0095] The antibacterial effects of honeysuckle essential oil, peppermint oil, thymol and sweet orange essential oil were evaluated by the punch method using Staphylococcus aureus as the test strain. First, 1.0×10 8 CFU / mL Staphylococcus aureus suspension, use a hole puncher to punch holes on the plate, add honeysuckle essential oil, peppermint oil, thymol and sweet orange essential oil respectively. Incubate the plate at 37℃ to observe the antibacterial effect of each component. Figure 2 .

[0096] Evaluation methods for antibacterial and antibacterial effects: Test strains: Staphylococcus aureus (ATCC 6538), Escherichia coli (8099), Candida albicans (ATCC10231)

[0097] Dilution: 0.03 mol / L phosphate buffer (PBS) (pH 7.2-7.4), culture medium: nutrient agar medium is used for the culture of Staphylococcus aureus and Escherichia coli, and Sabouraud agar medium is used for the culture of Candida albicans.

[0098] Take the 24h fresh slant culture of the test bacteria, wash it with PBS, and dilute it with PBS to about 5.0×10 5 CFU / mL~4.5×10 6 CFU / mL bacterial suspension for later use. Take a sterile test tube, add 5.0 mL of sample (use stock solution or diluent according to the instructions), place it in a 20℃±1℃ water bath for 5 minutes, then add 0.1 mL of test bacterial suspension, mix quickly and start timing immediately. Wait for the test bacteria and sample to interact for the specified time in the instructions, then take 1.0 mL of the test bacteria and sample mixture to inoculate 2 plates respectively, and pour the culture medium. At the same time, use PBS instead of the sample to perform a parallel test as a positive control. The number of colonies recovered in the positive control is 1.0×10 4 CFU / mL~9.0×10 4 CFU / mL. Take PBS and culture medium from the same batch as negative control.

[0099] All test samples and control samples were cultured at 36℃±1℃, bacterial propagules were cultured for 48h and the final results were observed; Candida albicans was cultured for 72h and the final results were observed. The test was repeated 3 times to observe the inhibitory effect. Figure 3 shown.

[0100] Table 4 Antibacterial test results of mouthwash

[0101] Note: “-” means the inhibition rate is ≤50%, which means no significant antibacterial effect; “+” means the inhibition rate is ≥50%~90%, which means there is an antibacterial effect; “++” means the inhibition rate is ≥90%, which means there is a strong antibacterial effect.

[0102] As shown in Table 4, the formula of 20% Chinese herbal extract, 0.05-0.1% thymol, 0.03% peppermint essential oil, 0.03% WS-3, 8% glycerol, 0.2% stevioside, 0.3% poloxamer 407, 0.2% methylparaben, and the balance of water has the most obvious antibacterial effect. It has a good inhibitory effect on Staphylococcus aureus, Escherichia coli, and Candida albicans.

[0103] The present invention uses honeysuckle, dandelion, licorice and mint, which are both medicinal and edible drugs, as raw materials to prepare a traditional Chinese medicine mouthwash with antibacterial effect. The prepared product has good taste, clarity, stability and pH value, meets the requirements of industry standards, and has a certain significant antibacterial effect. Compared with mainstream products with added chemical antibacterial agents, this product has the characteristics of plant extraction, safety and small side effects, and has certain application value.

[0104] Although the present invention has been described in detail above with general descriptions and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements can be made based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection claimed by the present invention.

Claims

1. A honeysuckle mouthwash with antibacterial effect, characterized in that: The mouthwash is composed of a Chinese medicine extract, honeysuckle recycled water and a mouthwash base, wherein the weight percentage of the Chinese medicine extract is 5-20%, the weight percentage of the honeysuckle dried recycled water is 5-40%, and the remainder is the mouthwash base; The Chinese medicine extract is prepared by mixing honeysuckle, dandelion and liquorice in a certain weight ratio and then extracting with water; The honeysuckle recycled water is the recycled condensed water collected after drying and processing the fresh honeysuckle, obtained by filtering and sterilizing; The mouthwash base is composed of the following ingredients in percentage by weight: 0.01-1% thymol, 0.01-0.3% plant essential oil, 0.01-1% cooling agent, 2-15% wetting agent, 0.04-1% sweetener, 0.1%-2% surfactant, 0.01-0.5% preservative, and the balance is water.

2. The honeysuckle mouthwash according to claim 1, characterized in that The traditional Chinese medicine extract is prepared by mixing honeysuckle, dandelion and liquorice in a weight ratio of (8-1:6-1:4-1) and then extracting with water.

3. The honeysuckle mouthwash according to claim 1 or 2, characterized in that The wetting agent is selected from one or more of glycerol, propylene glycol or ethanol; The surfactant includes poloxamer 407 and / or polysorbate 20 and / or 20 polyoxyethylene (20) castor oil and / or Tween; The sweetener is selected from steviol glycosides and / or mogrosides and / or erythritol; The cooling agent is selected from WS-3 and / or WS-23 and / or menthol; The plant essential oil is selected from one of honeysuckle essential oil, peppermint essential oil or sweet orange essential oil; The preservative is selected from methylparaben and / or propylparaben and / or sodium benzoate.

4. The honeysuckle mouthwash according to claims 1-3, characterized in that The honeysuckle recycled water is collected by drying fresh honeysuckle in a sealed dryer at 40-80° C. for 1-48 hours.

5. The method for preparing the mouthwash according to any one of claims 1 to 4, characterized in that: The following steps are involved: (1) Weigh the coarse powders of honeysuckle, dandelion and liquorice respectively, and heat and reflux the mixture with water 10 to 40 times the weight of the mixture; (2) Preliminarily filtering the extracted medicinal solution under reduced pressure until no impurities are precipitated, and then further adsorbing and filtering the pigments and impurities with an activated carbon filter to obtain a clarified medicinal solution, i.e., a Chinese medicine extract, as solution A; (3) Preliminary filtration and ultraviolet sterilization of the collected honeysuckle recycled water to obtain solution B; (4) Dissolve various water-insoluble ingredients in a certain amount of wetting agent in accordance with the formula requirements of the mouthwash base and mix them evenly to form solution C; (5) Then, the mixed solution A and solution C are added to the remaining drinking water under heating conditions, and solution B, preservatives, and sweeteners are added and mixed evenly to prepare honeysuckle mouthwash.

6. The method according to claim 4, characterized in that In step (1), the temperature of the heating reflux extraction is 75-95° C., and the extraction time is 40-90 minutes.

7. The method according to claim 4 or 5, characterized in that: In step (4), the heating temperature is 50-70° C., and the solution is stirred while heating until the solution is uniformly mixed.

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