Aminophilic aminobacter capable of efficiently degrading glyphosate and preparation method of microbial inoculum of aminophilic aminobacter

Abstract

The invention discloses a preparation method of an aminophilic aminobacter capable of efficiently degrading glyphosate and a microbial inoculum of the aminophilic aminobacter, the aminophilic aminobacter is classified and named as aminophilic aminobacter K2, the aminophilic aminobacter is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC32419. The invention further discloses a preparation method of the microbial inoculum of the aminophilic aminobacter capable of efficiently degrading glyphosate and a preparation method of the microbial inoculum of the aminophilic aminobacter capable of efficiently degrading glyphosate. The Aminobacter sp. Gram-negative bacterium provided by the invention can grow and breed by taking glyphosate as a unique carbon source; the glyphosate can be degraded in a complex matrix environment, can continuously and stably play a role in a complex matrix environment, not only shows high glyphosate degradation efficiency, but also has certain inorganic phosphorus degradation capacity. The strain K2 disclosed by the invention has a wide application prospect in repairing an environment polluted by glyphosate.

Description

Technical Field

[0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to a method for preparing an aminophilic bacillus capable of efficiently degrading glyphosate and a bacterial agent thereof. Background Art

[0002] Glyphosate (N-(phosphonomethyl)glycine) is a herbicide with lethality, broad spectrum, systemicity and conductivity. With the widespread planting of genetically modified crops, the prevalence of plant lethality during the harvest period of cash crops, pasture improvement, and no-till agriculture, glyphosate has become one of the most widely used herbicides in the world with the highest production volume and annual sales, accounting for about 25% of the global herbicide market. However, due to the potential biological toxicity of glyphosate, excessive or long-term intake of glyphosate-contaminated food can harm animal or human health. Due to excessive use and bioaccumulation, the presence of glyphosate has been detected in some environmental samples, with concentrations ranging from 1μg / L to 10mg / kg. In addition, residues of glyphosate and its main metabolite aminomethylphosphonic acid can also have certain negative effects on soil animals, non-target plants and microorganisms. Therefore, it is particularly necessary to take necessary measures to reduce the residues of glyphosate in the environment.

[0003] Microbial remediation technology is an efficient and green method to reduce glyphosate in soil. Researchers have isolated glyphosate-degrading microorganisms with different characteristics from a variety of environmental samples. However, some of these glyphosate-degrading microorganisms have poor survival and retention capabilities in complex natural environments, greatly reducing their effectiveness. Therefore, the development of efficient degradation bacteria is the key to eliminating the environmental hazards caused by glyphosate, and has important application value in the treatment of glyphosate-polluted environments. Summary of the invention

[0004] In view of the deficiencies pointed out in the above background technology, the present invention provides a method for preparing an aminophilic aminobacterium and a bacterial agent thereof for efficiently degrading glyphosate, aiming to solve the problems existing in the prior art in the above background technology.

[0005] To achieve the above object, the technical solution adopted by the present invention is:

[0006] Aminobacter aminovorans K2 is deposited in the General Microbiological Center of China Microorganism Culture Collection Administration Committee, with a deposit number of CGMCC No. 32419, a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and a deposit date of October 30, 2024. The Aminobacter aminovorans K2 comprises a gene sequence of SEQ ID NO: 1.

[0007] The present invention further provides an Aminobacter aminovorans bacterial agent, the active ingredient of which is the Aminobacter aminovorans K2.

[0008] The present invention further provides a method for preparing an aminobacterium aminophilum bacterial agent, comprising the following steps:

[0009] (1) Culture medium preparation

[0010] (a) Components of solid slant preservation medium and their final concentrations: peptone 5 g / L, beef extract 10 g / L, NaCl 5 g / L, yeast extract 5 g / L, glucose 5 g / L, agar 20 g / L, volume to 1 L, pH 7.0, autoclaved at 121°C for 20 min;

[0011] (b) Liquid culture medium components and their final concentrations: (NH4)2SO4 2 g / L, MgSO4·7H2O 0.2 g / L, FeSO4·7H2O 0.001 g / L, Na2HPO4 1.5 g / L, KH2PO4 1.5 g / L, CaCl2 0.01 g / L, sucrose 5 g / L, yeast extract 5 g / L, peptone 5 g / L, volume adjusted to 1 L, pH 7.0-7.2, sterilized by high pressure steam at 121°C for 20 min;

[0012] (2) Preparation of seed solution and bacterial agent: The above-mentioned Aminobacter aminovorans K2 was inoculated from the solid slant preservation culture medium into the liquid culture medium, and placed in an incubator at 30°C and 150 rpm for culturing for 24 h; Bacillus cereus seed solution was prepared and inoculated into the liquid culture medium, and placed in an incubator at 30°C and 150 rpm for expansion culture for 24 to 36 h to obtain the bacterial agent.

[0013] Preferably, the inoculation amount of the seed solution is 5%.

[0014] Preferably, the Aminobacter aminovorans K2 can grow in an inorganic salt culture medium with glyphosate as the sole carbon source, and the composition of the inorganic salt culture medium is: (NH4)2SO4 2.0g / L, MgSO4·7H2O0.2g / L, FeSO4·7H2O 0.001g / L, Na2HPO4 1.5g / L, KH2PO4 1.5g / L, CaCl2 0.01g / L, diluted to 1L with distilled water, pH 7.0-7.2, and sterilized by high-pressure steam at 121°C for 20min. In an inorganic salt culture medium, after 43 hours of cultivation, the growth of strain K2 in different glyphosate concentrations (50-1000 mg / L) can reach a stable period, and the glyphosate degradation rate can reach more than 97%; when the culture temperature is in the range of 20-35°C, strain K2 can completely degrade glyphosate within 48 hours; and when the pH is in the range of 5.5-8.5, it can completely degrade 600 mg / L glyphosate.

[0015] Preferably, the Aminobacter aminovorans K2 can grow in an inorganic salt medium in which glyphosate, sucrose, glucose, peptone and yeast extract co-exist, and the concentration of the sucrose, glucose, peptone and yeast extract is 5 g / L. In an inorganic salt medium containing 600 mg / L glyphosate, when 5 g / L of sucrose, glucose, peptone and yeast extract are added at the same time (i.e., sufficient exogenous nutrients are provided), the strain K2 can completely degrade glyphosate within 66 hours.

[0016] The above-mentioned Aminobacter aminovorans K2 can grow and reproduce with glyphosate as the only carbon source; when faced with complex substrates in the natural environment, strain K2 can also function continuously and stably. Strain K2 is a Gram-negative bacterium and contains enzyme genes related to resistance / degradation of glyphosate, such as aro, gat, and phn gene clusters. It not only shows high glyphosate degradation efficiency, but also has a certain ability to degrade inorganic phosphorus.

[0017] The invention discloses an application of an aminovorous bacterium Aminobacter in the degradation of glyphosate, wherein the aminovorous bacterium Aminobacter aminovorans K2 has high-efficiency glyphosate degradation characteristics and good environmental adaptability, and can achieve high-efficiency degradation of glyphosate under the condition of complex substrates. The strain K2 has a wide application prospect in repairing the environment polluted by glyphosate.

[0018] Compared with the shortcomings and deficiencies of the prior art, the present invention has the following beneficial effects:

[0019] The present invention provides an aminophilic aminobacillus, which has efficient glyphosate degradation characteristics and good environmental adaptability, and can achieve efficient degradation of glyphosate under the condition of complex substrates. The strain K2 has a wide application prospect for repairing the environment polluted by glyphosate. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The colony morphology of Aminobacterium aminophilum K2 provided in the embodiment of the present invention, wherein: Figure 1 (a) is the plate colony morphology of strain K2. Figure 1 (b) is a scanning electron microscopy image of strain K2.

[0021] Figure 2 This is a Gram staining diagram of Aminobacterium aminophilum K2 provided in an embodiment of the present invention.

[0022] Figure 3 The complete genome map of Aminobacterium aminophilum K2 provided in the embodiments of the present invention.

[0023] Figure 4 A phylogenetic tree of Aminobacterium aminophilum K2 provided in an embodiment of the present invention.

[0024] Figure 5 A diagram of inorganic phosphorus dissolution by Aminobacterium aminophilum K2 provided in an embodiment of the present invention.

[0025] Figure 6 The growth of Aminophilus aminobacterium K2 under different glyphosate concentrations provided in the embodiment of the present invention ( Figure 6 (a)) and degradation of glyphosate ( Figure 6 (b)).

[0026] Figure 7 The growth of Aminophilus aminobacterium K2 under different pH conditions provided in the embodiment of the present invention ( Figure 7 (a)) and degradation of glyphosate ( Figure 7 (b)).

[0027] Figure 8 The growth of Aminophilus aminobacterium K2 under different temperature conditions provided by the embodiment of the present invention ( Figure 8 (a)) and degradation of glyphosate ( Figure 8 (b)).

[0028] Fig. 9 The growth of Aminophilus aminobacterium K2 under different substrate conditions provided in the embodiments of the present invention ( Fig. 9 (a)) and degradation of glyphosate ( Fig. 9 (b)). DETAILED DESCRIPTION

[0029] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0030] Example 1

[0031] Screening, separation and purification of glyphosate-degrading bacteria - Aminobacter aminovorans K2: About 10g of vegetable soil samples containing glyphosate residues (glyphosate residue concentration 18.421μg / kg) were selected and added to 100mL of inorganic salt culture solution containing 50mg / L glyphosate, and cultured at 30℃ and 150rpm for 3 days. Then, they were gradually transferred to inorganic salt culture solutions containing 100, 200, 300, 400, 600, 800, and 1000mg / L glyphosate for 3-day cultivation. Each gradient of culture solution was spread on an inorganic salt solid plate containing 400mg / L glyphosate, incubated in a constant temperature incubator (30℃) for 3 days, and a single colony was picked up. The single colony was separated and purified by multiple plate streaking method. Figure 1 The colony morphology of strain K2 is shown, in which: Figure 1 (a) is the plate colony morphology of strain K2. Figure 1 (b) is a scanning electron microscope image of strain K2. The components and final concentrations of the inorganic salt culture medium are (NH4)2SO4 2g / L, MgSO4·7H2O 0.2g / L, FeSO4·7H2O 0.001g / L, Na2HPO4 1.5g / L, KH2PO4 1.5g / L, CaCl2 0.01g / L, pH value is 7.0-7.2, and a certain concentration of glyphosate is the only carbon source. Each purified strain was tested for glyphosate degradation, and finally strain K2 was obtained to have the best glyphosate degradation effect (refer to Figure 2 ). The whole genome was identified (the genome circos diagram of strain K2 is shown in Figure 3 The strain K2 was identified as the Gram-negative bacterium Aminobacter aminovorans, and also contained the enzyme gene aro, gat, phn gene clusters related to resistance / degradation of glyphosate. The genome information of the strain K2 is shown in Table 1, and the plasmid statistical information of the strain K2 is shown in Table 2.

[0032] Table 1 Genome information of glyphosate-degrading bacteria K2

[0033]

[0034] Table 2 Plasmid statistics of glyphosate-degrading bacteria K2

[0035]

[0036]

[0037] Aminobacter (AlphaProteobacteria) is a bacterium with the potential to degrade soil organic pollutants and good environmental adaptability. For example, it has been documented that Aminobacter aminovorans strian DXTK-010 can grow in an environment with 1,4-dioxane as the only carbon source and efficiently degrade 1,4-dioxane; Aminobacter aminovorans ZL-1006 can be used to treat sewage, feces and other organic pollutants and waste; Aminobacter aminovorans MDW-2 can degrade the carbamate insecticide methomyl; Aminobacter aminovorans KCTC 2477 can degrade nitrilotriacetic acid (NTA) and its metal ion complexes. The sequences of Aminobacteraminovorans K2 and the four strains described in the literature were compared by BLAST search function, and compared with the model strain Aminobacteraminovorans strain DSM 7048 and the exogenous strain Escherichia coli ATCC 11775. The phylogenetic tree was drawn using the neighbor-joining method of evolutionary tree construction on MEGA software. Figure 4 ) showed that the gene sequence of strain K2 was different from that of Aminobacter aminovorans strian DXTK, Aminobacteraminovorans ZL-1006, Aminobacter aminovorans MDW-2, and Aminobacter aminovorans KCTC 2477. Therefore, the glyphosate-degrading bacteria Aminobacter aminovorans K2 of the present invention belong to different species from the above strains.

[0038] The glyphosate-degrading bacteria obtained by the present invention are Aminobacter aminovorans, which is deposited in the General Microbiological Center of China Microorganism Culture Collection Committee with a deposit number of CGMCC No.32419, a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, and a deposit date of October 30, 2024. The classification name is Aminobacter aminovorans K2, and the gene sequence table of Aminobacter aminovorans K2 is shown in SEQ ID NO: 1.

[0039] Example 2

[0040] When glyphosate-degrading bacteria K2 were inoculated into inorganic phosphorus solid culture medium, a phosphorus-dissolving ring (such as Figure 5 The results show that strain K2 has certain inorganic phosphorus solubility characteristics. The genetic stability test of strain K2 was carried out by streaking the strain K2 on solid culture medium for 25 times, and the bacterial morphology, growth rate and glyphosate degradation ability were measured. It was found that there was no significant difference with the original strain, indicating that strain K2 had good genetic stability.

[0041] Example 3

[0042] Preparation of Bacillus aminophilus inoculum:

[0043] (1) Culture medium preparation

[0044] (a) Components of solid slant preservation medium and their final concentrations: peptone 5 g / L, beef extract 10 g / L, NaCl 5 g / L, yeast extract 5 g / L, glucose 5 g / L, agar 20 g / L, volume to 1 L, pH 7.0, autoclaved at 121°C for 20 min;

[0045] (b) Liquid culture medium components and their final concentrations: (NH4)2SO4 2 g / L, MgSO4·7H2O 0.2 g / L, FeSO4·7H2O 0.001 g / L, Na2HPO4 1.5 g / L, KH2PO4 1.5 g / L, CaCl2 0.01 g / L, sucrose 5 g / L, yeast extract 5 g / L, peptone 5 g / L, volume adjusted to 1 L, pH 7.0-7.2, sterilized by high pressure steam at 121°C for 20 min;

[0046] (2) Preparation of seed solution and bacterial agent: Aminobacter aminovorans K2 was inoculated from a solid slant preservation medium into a liquid culture medium, and the culture was placed in an incubator at 30°C and 150 rpm for 24 h. Bacillus cereus seed solution was prepared and inoculated into a liquid culture medium, and the culture was placed in an incubator at 30°C and 150 rpm for 24 to 36 h to obtain a bacterial agent.

[0047] Example 4

[0048] Growth of strain K2 under different glyphosate concentrations and glyphosate degradation ability test: strain K2 was inoculated with 5% inoculum into 20mL inorganic salt medium / 50mL Erlenmeyer flask containing different glyphosate concentrations (50, 200, 400, 600, 800, 1000 mg / L), and cultured at 30°C and 150rpm. Each treatment consisted of 45 parallel samples. Starting from 0h, 3 parallel samples were taken from each treatment at intervals for OD 600 The glyphosate content was determined by spectrophotometry according to the national standard GB / T20684-2017, and the OD value of the culture medium was measured by spectrophotometry. 600 Among them, the components and final concentrations of the inorganic salt culture medium are (NH4)2SO4 2g / L, MgSO4·7H2O 0.2g / L, FeSO4·7H2O0.001g / L, Na2HPO4 1.5g / L, KH2PO4 1.5g / L, CaCl2 0.01g / L, pH value is equal to 7.0-7.2, and a certain concentration of glyphosate is the only carbon source.

[0049] The results are as follows Figure 6 As shown, Figure 6 (a) is the growth of strain K2 under different glyphosate concentrations. Figure 6 (b) shows the degradation of glyphosate by strain K2 at different glyphosate concentrations. It shows that the growth of strain K2 cells exhibits three different phases: lag phase, proliferation phase, and decline phase. When the initial glyphosate concentration was 1079.24 mg / L (measured value) and cultured for 37 h, the cell growth OD 600The value reached a maximum value of 0.29. In addition, after 43 hours of cultivation, the growth of strain K2 in different glyphosate concentrations can reach a stable period. As the initial glyphosate concentration increased from 67.33 mg / L (measured value) to 1079.24 mg / L (measured value), the lag period increased, and the degradation rate of glyphosate reached more than 97% after 43 hours of cultivation. Under the optimal culture conditions in the laboratory, some degradation bacteria can degrade about 90% of glyphosate within 5 days, such as Ochrobactrum sp.GDOS (initial glyphosate concentration in the culture medium is 504 mg / L, degradation time is 2.5 days), Ochrobactrum intermedium Sq20 (initial glyphosate concentration in the culture medium is 500 mg / L, degradation time is 4 days), Comamonas odontotermitis P2 (initial glyphosate concentration in the culture medium is 1.5 g / L, degradation time is 5 days), Bacillus cereus CB4 (initial glyphosate concentration in the soil is 6 g / L, degradation time is 5 days) and Stenotrophomonasacidaminiphila Y4B (initial glyphosate concentration in the soil is 400 mg / L, degradation time is 5 days), etc. Compared with these strains, strain K2 can degrade 97% glyphosate within 43 hours (initial glyphosate concentration in the culture medium is 1079.24 mg / L), indicating that strain K2 can be used to degrade glyphosate more efficiently.

[0050] Example 5

[0051] Growth of glyphosate-degrading bacteria K2 under different pH conditions and glyphosate degradation ability test: strain K2 was inoculated with 5% inoculum into 20mL inorganic salt medium / 50mL Erlenmeyer flasks with different pH values ​​(pH values ​​were 4.5, 5.5, 6.5, 7.5, 8.5, 9.5, pH value adjustment was made with 1mol / L HCl solution and 1mol / L NaOH solution), and the initial glyphosate concentration was set to 600mg / L, and cultured at 30℃ and 150rpm. Each treatment consisted of 54 parallel samples. Starting from 0h, 3 parallel samples were taken from each treatment at intervals for OD 600 The glyphosate content was determined by spectrophotometry according to the national standard GB / T20684-2017, and the OD value of the culture medium was measured by spectrophotometry. 600 Among them, the components and final concentrations of the inorganic salt culture medium are (NH4)2SO4 2g / L, MgSO4·7H2O 0.2g / L, FeSO4·7H2O0.001g / L, Na2HPO4 1.5g / L, KH2PO4 1.5g / L, CaCl2 0.01g / L, and a certain concentration of glyphosate is the only carbon source.

[0052] The results are as follows Figure 7 As shown, Figure 7 (a) is the growth of strain K2 under different pH conditions. Figure 7 (b) shows the degradation of glyphosate by strain K2 under different pH conditions. It shows that 600 mg / L glyphosate can be completely degraded in the pH range of 5.5 to 8.5. In the initial pH range of 5.5 to 8.5, strain K2 showed a rapid degradation rate and growth; after 42 hours of cultivation, glyphosate was completely degraded; under the condition of pH 5.5, OD 600 The value reached a maximum value of 0.16. Under the initial pH values ​​of 4.5 and 9.5, the pH of the culture medium had an adverse effect on the growth of the strain and the degradation of glyphosate. However, it is worth noting that under the initial pH value of 9.5, strain K2 still showed stubborn degradation resistance. After a lag period of about 60 hours, strain K2 cells proliferated rapidly and degraded glyphosate by 95.54% within 144 hours. As glyphosate was degraded, from 60h to 144h, OD 600 These results fully demonstrate that strain K2 has the potential to be used in remediating glyphosate-contaminated environments, especially in alkaline and oligotrophic environments.

[0053] Example 6

[0054] Growth and glyphosate degradation ability test of glyphosate-degrading bacteria K2 under different temperature conditions: Inoculate strain K2 with 5% inoculum into 20mL inorganic salt medium / 50mL Erlenmeyer flask containing an initial glyphosate concentration of 600mg / L, and culture at different temperatures of 15, 20, 25, 30, 35, 40℃ and 150rpm. Each treatment has 45 parallel samples. Starting from 0h, 3 parallel samples are taken from each treatment at intervals for OD 600 The glyphosate content was determined by spectrophotometry according to the national standard GB / T20684-2017, and the OD value of the culture medium was measured by spectrophotometry. 600 Among them, the components and final concentrations of the inorganic salt culture medium are (NH4)2SO4 2g / L, MgSO4·7H2O 0.2g / L, FeSO4·7H2O0.001g / L, Na2HPO4 1.5g / L, KH2PO4 1.5g / L, CaCl2 0.01g / L, pH value is equal to 7.0-7.2, and a certain concentration of glyphosate is the only carbon source.

[0055] The results are as follows Figure 8 As shown, Figure 8 (a) is the growth of strain K2 under different temperature conditions. Figure 8(b) shows the degradation of glyphosate by strain K2 under different temperature conditions. It shows that under the optimum culture temperature of 30°C, the time required for complete degradation of glyphosate is the shortest (36 h). After 12 h, OD 600 The value reached a maximum of 0.16. When the culture temperature ranged from 20℃ to 35℃, strain K2 was able to completely degrade glyphosate within 66h. 600 The value reached a maximum value of 0.20, but its degradation rate was lower than the degradation rate at 25℃ and 30℃. When the culture temperature was reduced to 15℃, the lag phase increased and the glyphosate degradation time was extended to 114h. However, at 40℃, the growth of strain K2 cells was very slow and the glyphosate degradation rate was also very low. Therefore, the culture temperature of 20℃ to 35℃ is the suitable temperature for strain K2 to degrade glyphosate, and the optimum temperature is 30℃.

[0056] Example 7

[0057] Growth of glyphosate-degrading bacteria K2 under different substrate addition conditions and glyphosate degradation ability test: strain K2 can grow with glyphosate as the sole carbon source, but there may be some additional or naturally existing available substrates in the polluted environment, which will have an uncertain impact on the bioremediation of strain K2. Therefore, this embodiment studies the effect of adding four exogenous energy substances on the degradation of glyphosate by strain K2. Strain K2 was inoculated with an inoculum of 5% into a 20mL inorganic salt medium / 50mL triangular flask containing an initial glyphosate concentration of 600mg / L, and 5g / L glucose, 5g / L sucrose, 5g / L yeast extract, 5g / L peptone, and full nutrition (5g / L glucose + 5g / L sucrose + 5g / L yeast extract + 5g / L peptone) were added respectively. No exogenous energy substances were added as the control (CK), and the culture was shaken at 30°C and 150rpm. Each treatment consisted of 33 parallel samples. Starting from 0 h, 3 parallel samples were taken from each treatment at intervals for OD 600 The glyphosate content was determined by spectrophotometry according to the national standard GB / T20684-2017, and the OD value of the culture medium was measured by spectrophotometry. 600 Among them, the components and final concentrations of the inorganic salt culture medium are (NH4)2SO4 2g / L, MgSO4·7H2O0.2g / L, FeSO4·7H2O 0.001g / L, Na2HPO4 1.5g / L, KH2PO4 1.5g / L, CaCl2 0.01g / L, pH value is equal to 7.0-7.2, and a certain concentration of glyphosate is the only carbon source.

[0058] The results are as follows Fig. 9 As shown, Fig. 9 (a) is the growth of strain K2 under different substrate conditions. Fig. 9 (b) shows the degradation of glyphosate by strain K2 under different substrate conditions, indicating that the addition of these four exogenous substances promotes the growth of strain K2 to varying degrees. When sucrose, glucose, peptone, and yeast extract are added at the same time (i.e., sufficient exogenous nutrients are given), strain K2 can completely degrade glyphosate within 66 hours. This shows that strain K2 can grow and reproduce with glyphosate as the only carbon source; when faced with complex substrates in the natural environment, strain K2 can also continuously and stably perform degradation.

[0059] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A bacterium aminophilum, characterized in that The aminobacterium aminovorans is classified and named as Aminobacter aminovorans K2, and is deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, with a deposit number of CGMCC32419 and a deposit date of October 30, 2024. The aminobacterium aminovorans K2 comprises the gene sequence of SEQ ID NO:

1.

2. A bacterial agent of Aminophilus aminophilus, characterized in that The active ingredient of the bacterial agent is the Aminobacter aminovorans K2 described in claim 1.

3. A method for preparing the aminobacterium aminophilum bacterial agent according to claim 2, characterized in that: The following steps are involved: (1) Culture medium preparation (a) Components of solid preservation medium and their final concentrations: peptone 5 g / L, beef extract 10 g / L, NaCl 5 g / L, yeast extract 5 g / L, glucose 5 g / L, agar 20 g / L, volume to 1 L, pH 7.0, autoclaved at 121°C for 20 min; (b) Liquid culture medium components and their final concentrations: (NH4)2SO4 2 g / L, MgSO4·7H2O 0.2 g / L, FeSO4·7H2O0.001 g / L, Na2HPO4 1.5 g / L, KH2PO4 1.5 g / L, CaCl2 0.01 g / L, sucrose 5 g / L, yeast extract 5 g / L, peptone 5 g / L, volume adjusted to 1 L, pH 7.0-7.2, sterilized by high pressure steam at 121°C for 20 min; (2) Preparation of seed solution and inoculant The Aminobacter aminovorans K2 is inoculated into a liquid culture medium from a solid slant, and is placed in a 30°C incubator at 150 rpm for 24 hours; a Bacillus cereus seed solution is prepared and inoculated into a liquid culture medium, and is placed in a 30°C incubator at 150 rpm for 24-36 hours of expansion culture to obtain a bacterial agent.

4. The method for preparing the aminobacterium aminophilum bacterial agent according to claim 3, characterized in that: The inoculation amount of the seed liquid is 5%.

5. The method for preparing the aminobacterium aminophilum bacterial agent according to claim 3, characterized in that: The Aminobacter aminovorans K2 can grow in an inorganic salt culture medium with glyphosate as the sole carbon source. The inorganic salt culture medium comprises: (NH4)2SO4 2.0 g / L, MgSO4·7H2O 0.2 g / L, FeSO4·7H2O 0.001 g / L, Na2HPO4 1.5 g / L, KH2PO4 1.5 g / L, CaCl2 0.01 g / L, the volume is adjusted to 1 L with distilled water, the pH value is 7.0-7.2, and the culture medium is sterilized by high-pressure steam at 121°C for 20 min.

6. The method for preparing the aminobacterium aminophilum bacterial agent according to claim 5, characterized in that: In the inorganic salt culture medium, the glyphosate concentration is 50-1000 mg / L.

7. The method for preparing the aminobacterium aminophilum bacterial agent according to claim 5, characterized in that: In the inorganic salt culture medium, the culture temperature is 20-35°C.

8. The method for preparing the aminobacterium aminophilum bacterial agent according to claim 5, characterized in that: The pH of the inorganic salt culture medium is 5.5-8.

5.

9. The method for preparing the aminobacterium aminophilum bacterial agent according to claim 3, characterized in that: The Aminobacter aminovorans K2 can grow in an inorganic salt culture medium in the presence of glyphosate, sucrose, glucose, peptone and yeast extract, and has the function of degrading glyphosate. The concentration of the sucrose, glucose, peptone and yeast extract is 5 g / L.

10. Use of the aminobacterium aminophilum bacterial agent as claimed in claim 2 in degrading glyphosate.

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